Data related to the manuscript ÒImaging brain tissue architecture across millimeter to nanometer scalesÓ by Michalska et al., 2023. The repository includes imaging data and deep-learning models for automated synapse detection. Designation of datasets is according to the figure numbering in the manuscript, where also a detailed description of the experimental parameters and data processing can be found. Please cite the paper when re-using any of the material. The material provided here is provided under the CC BY-NC-SA (4.0) license.