---
OA_type: closed access
_id: '20972'
abstract:
- lang: eng
  text: Small amounts of stress are thought to have beneficial effects. A new study
    reports a mechanism by which the psychedelic drug, psilocybin, causes acute release
    of stress hormones, despite its known long-term anti-anxiety effects.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Hakan
  full_name: Kücükdereli, Hakan
  id: 5d5f6ea4-ef9e-11f0-a10a-85e12a3552af
  last_name: Kücükdereli
- first_name: Amelia May Barnett
  full_name: Douglass, Amelia May Barnett
  id: de5f6fda-80fb-11ef-996f-a8c4ecd8e289
  last_name: Douglass
  orcid: 0000-0001-5398-6473
citation:
  ama: 'Kücükdereli H, Douglass AM. Neuroscience: What doesn’t kill you makes you
    stronger. <i>Current Biology</i>. 2026;36(1):R27-R29. doi:<a href="https://doi.org/10.1016/j.cub.2025.11.056">10.1016/j.cub.2025.11.056</a>'
  apa: 'Kücükdereli, H., &#38; Douglass, A. M. (2026). Neuroscience: What doesn’t
    kill you makes you stronger. <i>Current Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.cub.2025.11.056">https://doi.org/10.1016/j.cub.2025.11.056</a>'
  chicago: 'Kücükdereli, Hakan, and Amelia M. Douglass. “Neuroscience: What Doesn’t
    Kill You Makes You Stronger.” <i>Current Biology</i>. Elsevier, 2026. <a href="https://doi.org/10.1016/j.cub.2025.11.056">https://doi.org/10.1016/j.cub.2025.11.056</a>.'
  ieee: 'H. Kücükdereli and A. M. Douglass, “Neuroscience: What doesn’t kill you makes
    you stronger,” <i>Current Biology</i>, vol. 36, no. 1. Elsevier, pp. R27–R29,
    2026.'
  ista: 'Kücükdereli H, Douglass AM. 2026. Neuroscience: What doesn’t kill you makes
    you stronger. Current Biology. 36(1), R27–R29.'
  mla: 'Kücükdereli, Hakan, and Amelia M. Douglass. “Neuroscience: What Doesn’t Kill
    You Makes You Stronger.” <i>Current Biology</i>, vol. 36, no. 1, Elsevier, 2026,
    pp. R27–29, doi:<a href="https://doi.org/10.1016/j.cub.2025.11.056">10.1016/j.cub.2025.11.056</a>.'
  short: H. Kücükdereli, A.M. Douglass, Current Biology 36 (2026) R27–R29.
corr_author: '1'
date_created: 2026-01-11T23:01:33Z
date_published: 2026-01-05T00:00:00Z
date_updated: 2026-01-12T10:09:13Z
day: '05'
department:
- _id: AmDo
- _id: SiHi
doi: 10.1016/j.cub.2025.11.056
external_id:
  pmid:
  - '41494523'
intvolume: '        36'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: R27-R29
pmid: 1
publication: Current Biology
publication_identifier:
  eissn:
  - 1879-0445
  issn:
  - 0960-9822
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Neuroscience: What doesn’t kill you makes you stronger'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '21744'
abstract:
- lang: eng
  text: The paraventricular hypothalamus (PVH) controls behavioral and physiologic
    processes, including appetite, social behavior, autonomic outflow, and pituitary
    hormone secretion. However, molecular markers for centrally projecting PVH neuron
    populations remain largely undefined, and a complete census of PVH cell types
    has not been established. Therefore, we performed extensive single-cell/nucleus
    RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence
    in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic
    atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding
    areas. Additionally, projection-based profiling identified neurons that project
    to the parabrachial region (PB) and spinal cord, helping to determine PVH populations
    that regulate satiety and sympathetic nervous system activity, respectively. Notably,
    activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and
    silencing them causes obesity. Together, this atlas contributes high-resolution
    PVH spatial and circuit-based gene expression profiles, representing a valuable
    resource for the field of homeostasis.
acknowledgement: "We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford\r\nSaper,
  as well as the Lowell, Tsai, and Resch laboratories for helpful discussions;\r\nAlysia
  Berns, Jia Yu, and Yanfang Li for technical support; the BNORC\r\nFunctional Genomics
  and Bioinformatics Core (P30DK046200) and the Iowa\r\nInstitute for Human Genetics
  Genomics Division (IIHG, RRID: SCR_023422)\r\nfor helpful discussions and technical
  assistance with sc/snRNA-seq; Zachary\r\nNiziolek and the Bauer Core Facility at
  Harvard University, the BIDMC Flow Cytometry\r\nCore, and Heath Vignes, Michael
  Shey, and Thomas Kaufman of the\r\nFlow Cytometry Facility at the University of
  Iowa Carver College of Medicine\r\nfor helpful discussions and technical support;
  the ICCB-Longwood Screening\r\nFacility of Harvard Medical School for assistance
  with the snRNA-seq\r\nexperiments; Dr. Sayak Mitter and Vizgen support for technical
  assistance\r\nwith the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin\r\nfor
  their assistance with MERSCOPE experiments within the Iowa\r\nNeuroBank Core in
  the Iowa Neuroscience Institute at the University of Iowa\r\nCarver College of Medicine.
  This research was funded by the following NIH\r\ngrants to L.T.T.: R01DK128406;
  to B.B.L.: R01DK075632, R01DK134427,\r\nand R01DK096010; to J.M.R.: R00HL144923
  and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by
  a pharmacological\r\nsciences predoctoral training grant T32GM144636. Additional
  funding\r\nto J.M.R. came from the American Heart Association (AHA 935362), a University\r\nof
  Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and\r\nFeasibility
  Catalyst Grant, and an Iowa Neuroscience Institute Early Stage\r\nInvestigator award
  from the Carver Trust. Y.L. was supported by a predoctoral\r\nfellowship from the
  American Heart Association (AHA 25PRE1372983). A.M.D.\r\nwas supported by a postdoctoral
  fellowship from the Charles A. King Trust."
article_number: '116904'
article_processing_charge: Yes
article_type: original
author:
- first_name: Yuxi
  full_name: Li, Yuxi
  last_name: Li
- first_name: Trevor C.
  full_name: Butler, Trevor C.
  last_name: Butler
- first_name: Stefano
  full_name: Nardone, Stefano
  last_name: Nardone
- first_name: Christopher L.
  full_name: Jacobs, Christopher L.
  last_name: Jacobs
- first_name: Amelia May Barnett
  full_name: Douglass, Amelia May Barnett
  id: de5f6fda-80fb-11ef-996f-a8c4ecd8e289
  last_name: Douglass
  orcid: 0000-0001-5398-6473
- first_name: Joseph C.
  full_name: Madara, Joseph C.
  last_name: Madara
- first_name: Miriam C.
  full_name: McDonough, Miriam C.
  last_name: McDonough
- first_name: Jenkang
  full_name: Tao, Jenkang
  last_name: Tao
- first_name: Elijah D.
  full_name: Lowenstein, Elijah D.
  last_name: Lowenstein
- first_name: Luhong
  full_name: Wang, Luhong
  last_name: Wang
- first_name: Deepti
  full_name: Pant, Deepti
  last_name: Pant
- first_name: Samuel J.
  full_name: Walker, Samuel J.
  last_name: Walker
- first_name: Annette
  full_name: Wang, Annette
  last_name: Wang
- first_name: Harini
  full_name: Srinivasan, Harini
  last_name: Srinivasan
- first_name: Zongfang
  full_name: Yang, Zongfang
  last_name: Yang
- first_name: John N.
  full_name: Campbell, John N.
  last_name: Campbell
- first_name: Linus T.
  full_name: Tsai, Linus T.
  last_name: Tsai
- first_name: Bradford B.
  full_name: Lowell, Bradford B.
  last_name: Lowell
- first_name: Jon M.
  full_name: Resch, Jon M.
  last_name: Resch
citation:
  ama: Li Y, Butler TC, Nardone S, et al. A spatial and projection-based transcriptomic
    atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. 2026;45(2).
    doi:<a href="https://doi.org/10.1016/j.celrep.2025.116904">10.1016/j.celrep.2025.116904</a>
  apa: Li, Y., Butler, T. C., Nardone, S., Jacobs, C. L., Douglass, A. M., Madara,
    J. C., … Resch, J. M. (2026). A spatial and projection-based transcriptomic atlas
    of paraventricular hypothalamic cell types. <i>Cell Reports</i>. Elsevier. <a
    href="https://doi.org/10.1016/j.celrep.2025.116904">https://doi.org/10.1016/j.celrep.2025.116904</a>
  chicago: Li, Yuxi, Trevor C. Butler, Stefano Nardone, Christopher L. Jacobs, Amelia
    M. Douglass, Joseph C. Madara, Miriam C. McDonough, et al. “A Spatial and Projection-Based
    Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>.
    Elsevier, 2026. <a href="https://doi.org/10.1016/j.celrep.2025.116904">https://doi.org/10.1016/j.celrep.2025.116904</a>.
  ieee: Y. Li <i>et al.</i>, “A spatial and projection-based transcriptomic atlas
    of paraventricular hypothalamic cell types,” <i>Cell Reports</i>, vol. 45, no.
    2. Elsevier, 2026.
  ista: Li Y, Butler TC, Nardone S, Jacobs CL, Douglass AM, Madara JC, McDonough MC,
    Tao J, Lowenstein ED, Wang L, Pant D, Walker SJ, Wang A, Srinivasan H, Yang Z,
    Campbell JN, Tsai LT, Lowell BB, Resch JM. 2026. A spatial and projection-based
    transcriptomic atlas of paraventricular hypothalamic cell types. Cell Reports.
    45(2), 116904.
  mla: Li, Yuxi, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular
    Hypothalamic Cell Types.” <i>Cell Reports</i>, vol. 45, no. 2, 116904, Elsevier,
    2026, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116904">10.1016/j.celrep.2025.116904</a>.
  short: Y. Li, T.C. Butler, S. Nardone, C.L. Jacobs, A.M. Douglass, J.C. Madara,
    M.C. McDonough, J. Tao, E.D. Lowenstein, L. Wang, D. Pant, S.J. Walker, A. Wang,
    H. Srinivasan, Z. Yang, J.N. Campbell, L.T. Tsai, B.B. Lowell, J.M. Resch, Cell
    Reports 45 (2026).
date_created: 2026-04-16T13:51:29Z
date_published: 2026-02-24T00:00:00Z
date_updated: 2026-05-04T12:00:31Z
day: '24'
ddc:
- '570'
department:
- _id: AmDo
doi: 10.1016/j.celrep.2025.116904
external_id:
  pmid:
  - '41581146'
file:
- access_level: open_access
  checksum: 82098dd9d0ca609119f9f2c6beb4fc1e
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-04T11:58:51Z
  date_updated: 2026-05-04T11:58:51Z
  file_id: '21793'
  file_name: 2026_CellReports_Li.pdf
  file_size: 38532865
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T11:58:51Z
has_accepted_license: '1'
intvolume: '        45'
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic
  cell types
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
OA_place: repository
OA_type: green
_id: '21955'
abstract:
- lang: eng
  text: 'AgRP neurons cause hunger, the drive to seek and consume food. Their activation
    by fasting is key for survival and is thought to be triggered by feedback when
    energy stores are low. However, we know that environmental cues can also regulate
    AgRP neurons since cues that predict future food intake rapidly inhibit AgRP neurons,
    but is the converse true: can the prediction of future fasting rapidly activate
    AgRP neurons? Here, we show in mice that such rapid fasting activation of AgRP
    neurons does occur. This rapid activation is driven by excitatory input from paraventricular
    hypothalamic (PVH) neurons expressing Sim2, which are bidirectionally sensitive
    to predictions of future energy state. Thus, cognitively processed contextual
    information conveyed by PVHSim2 neurons strongly activates AgRP neurons. Lastly,
    chronic silencing of PVHSim2 neurons causes persistent hypophagia. This PVHSim2-to-AgRP-neuron
    circuit, by anticipating and preventing negative energy balance, provides an important
    new dimension of hunger regulation.'
acknowledgement: "We thank all members of the B.B.L. laboratory for helpful discussions.
  We\r\nthank the BADERC and BNORC transgenic cores (NIH P30DK057521 and\r\nP30DK046200)
  for performing embryo injections to generate knockin mouse\r\nlines. We also thank
  the BIDMC Energy Balance Core (supported by NIH\r\nS10OD028635 and the Boston Area
  Diabetes Endocrinology Research Centers, P30DK135043), where Marissa Cortopassi
  performed indirect calorimetry experiments and Alexander Banks assisted with data
  analysis and interpretation. Confocal imaging was performed at BIDMC’s Confocal
  Imaging\r\nCore. We thank Chen Wu for assistance in designing knockin mouse lines.\r\nThis
  work was supported by the NIH (R01DK134427, R01DK096010, and\r\nR01DK075632 to B.B.L.).
  Authors were supported by an EMBO Long-Term\r\nFellowship (770-2018, S.J.W.), a
  T32 Postdoctoral Training Fellowship\r\n(5T32DK007516, E.D.L.), the Charles A. King
  Trust Postdoctoral Research\r\nFellowship program (A.M.D.), and a K99 Career Development
  Award\r\n(K99HL144923, J.M.R.)."
article_processing_charge: No
article_type: original
author:
- first_name: Samuel J.
  full_name: Walker, Samuel J.
  last_name: Walker
- first_name: Elijah D.
  full_name: Lowenstein, Elijah D.
  last_name: Lowenstein
- first_name: Amelia May Barnett
  full_name: Douglass, Amelia May Barnett
  id: de5f6fda-80fb-11ef-996f-a8c4ecd8e289
  last_name: Douglass
  orcid: 0000-0001-5398-6473
- first_name: Callum M.P.
  full_name: Thomas, Callum M.P.
  last_name: Thomas
- first_name: Joseph C.
  full_name: Madara, Joseph C.
  last_name: Madara
- first_name: Hakan
  full_name: Kucukdereli, Hakan
  last_name: Kucukdereli
- first_name: Eunice A.
  full_name: Barbosa-Meillon, Eunice A.
  last_name: Barbosa-Meillon
- first_name: Jenkang
  full_name: Tao, Jenkang
  last_name: Tao
- first_name: Jon M.
  full_name: Resch, Jon M.
  last_name: Resch
- first_name: Bradford B.
  full_name: Lowell, Bradford B.
  last_name: Lowell
citation:
  ama: Walker SJ, Lowenstein ED, Douglass AM, et al. A hypothalamic circuit for anticipating
    future changes in energy balance. <i>Neuron</i>. doi:<a href="https://doi.org/10.1016/j.neuron.2026.05.010">10.1016/j.neuron.2026.05.010</a>
  apa: Walker, S. J., Lowenstein, E. D., Douglass, A. M., Thomas, C. M. P., Madara,
    J. C., Kucukdereli, H., … Lowell, B. B. (n.d.). A hypothalamic circuit for anticipating
    future changes in energy balance. <i>Neuron</i>. Elsevier. <a href="https://doi.org/10.1016/j.neuron.2026.05.010">https://doi.org/10.1016/j.neuron.2026.05.010</a>
  chicago: Walker, Samuel J., Elijah D. Lowenstein, Amelia M. Douglass, Callum M.P.
    Thomas, Joseph C. Madara, Hakan Kucukdereli, Eunice A. Barbosa-Meillon, Jenkang
    Tao, Jon M. Resch, and Bradford B. Lowell. “A Hypothalamic Circuit for Anticipating
    Future Changes in Energy Balance.” <i>Neuron</i>. Elsevier, n.d. <a href="https://doi.org/10.1016/j.neuron.2026.05.010">https://doi.org/10.1016/j.neuron.2026.05.010</a>.
  ieee: S. J. Walker <i>et al.</i>, “A hypothalamic circuit for anticipating future
    changes in energy balance,” <i>Neuron</i>. Elsevier.
  ista: Walker SJ, Lowenstein ED, Douglass AM, Thomas CMP, Madara JC, Kucukdereli
    H, Barbosa-Meillon EA, Tao J, Resch JM, Lowell BB. A hypothalamic circuit for
    anticipating future changes in energy balance. Neuron.
  mla: Walker, Samuel J., et al. “A Hypothalamic Circuit for Anticipating Future Changes
    in Energy Balance.” <i>Neuron</i>, Elsevier, doi:<a href="https://doi.org/10.1016/j.neuron.2026.05.010">10.1016/j.neuron.2026.05.010</a>.
  short: S.J. Walker, E.D. Lowenstein, A.M. Douglass, C.M.P. Thomas, J.C. Madara,
    H. Kucukdereli, E.A. Barbosa-Meillon, J. Tao, J.M. Resch, B.B. Lowell, Neuron
    (n.d.).
date_created: 2026-06-08T09:24:25Z
date_published: 2026-06-03T00:00:00Z
date_updated: 2026-06-16T08:35:11Z
day: '03'
department:
- _id: AmDo
doi: 10.1016/j.neuron.2026.05.010
external_id:
  pmid:
  - '42235510'
keyword:
- hunger
- hypothalamus
- AGRP neurons
- neuroscience
- metabolism
- homeostasis
- feeding
- food intake
- energy balance
- appetite
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2025.09.27.678865
month: '06'
oa: 1
oa_version: Preprint
pmid: 1
publication: Neuron
publication_identifier:
  eissn:
  - ' 1097-4199'
  issn:
  - 0896-6273
publication_status: inpress
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A hypothalamic circuit for anticipating future changes in energy balance
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...