[{"oa_version":"Published Version","status":"public","_id":"21860","acknowledgement":"The technical assistance by Tanja Wagner and Elena Lilliu is gratefully acknowledged. This research was funded in whole or in part by the Austrian Science Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access funding provided by Medical University of Vienna and the Austrian Science Fund (FWF). Deposited in PMC for immediate release.","citation":{"ama":"Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. 2026;139(8). doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>","ista":"Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H. 2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. 139(8), jcs264420.","ieee":"V. Goeschl <i>et al.</i>, “α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures,” <i>Journal of Cell Science</i>, vol. 139, no. 8. The Company of Biologists, 2026.","apa":"Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V., &#38; Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>","chicago":"Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>. The Company of Biologists, 2026. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>.","short":"V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H. Kubista, Journal of Cell Science 139 (2026).","mla":"Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>, vol. 139, no. 8, jcs264420, The Company of Biologists, 2026, doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>."},"year":"2026","publisher":"The Company of Biologists","day":"27","file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2026-05-12T06:27:54Z","relation":"main_file","checksum":"8db35c97588c2f6ef88c7e8d5924cf8c","access_level":"open_access","file_size":1957057,"date_created":"2026-05-12T06:27:54Z","file_name":"2026_JourCellScience_Goeschl.pdf","file_id":"21861","success":1}],"intvolume":"       139","external_id":{"pmid":["41834724"]},"issue":"8","date_updated":"2026-05-12T06:40:18Z","file_date_updated":"2026-05-12T06:27:54Z","abstract":[{"lang":"eng","text":"Glutamate excitotoxicity is a cell death mechanism triggered by accumulation of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex (αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point controlling glutamate formation and its consumption as a fuel. Hence, modulation of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic effects. To address this hypothesis, hippocampal neurons in primary co-culture with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover, NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA receptors involved mGluR1 activation and was accompanied by enhanced neuronal discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses evoked by glutamate application. These results suggest that mGluR1-mediated potentiation of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might exacerbate glutamate excitotoxicity."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","fulldoi":"https://doi.org/10.1242/jcs.264420","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"date_published":"2026-04-27T00:00:00Z","OA_type":"hybrid","has_accepted_license":"1","PlanS_conform":"1","OA_place":"publisher","department":[{"_id":"Bio"}],"volume":139,"language":[{"iso":"eng"}],"publication_status":"published","month":"04","date_created":"2026-05-11T10:52:27Z","article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","scopus_import":"1","author":[{"first_name":"Vanessa","full_name":"Goeschl, Vanessa","last_name":"Goeschl"},{"first_name":"Matej","last_name":"Hotka","full_name":"Hotka, Matej"},{"first_name":"Bernhard","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","last_name":"Hochreiter","full_name":"Hochreiter, Bernhard"},{"first_name":"Karlheinz","full_name":"Hilber, Karlheinz","last_name":"Hilber"},{"first_name":"Stefan","last_name":"Boehm","full_name":"Boehm, Stefan"},{"full_name":"Kozlov, Andrey V.","last_name":"Kozlov","first_name":"Andrey V."},{"first_name":"Helmut","last_name":"Kubista","full_name":"Kubista, Helmut"}],"publication":"Journal of Cell Science","doi":"10.1242/jcs.264420","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"title":"α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures","oa":1,"article_number":"jcs264420"},{"article_processing_charge":"Yes (via OA deal)","date_created":"2026-07-13T10:44:55Z","supplementarymaterial":"yes","quality_controlled":"1","project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"publication_status":"epub_ahead","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"month":"06","type":"journal_article","doi":"10.1111/jipb.70309","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/jipb.70309"}],"oa":1,"title":"Auxin response and PIN‐mediated transport in chlorophyte algae","pmid":1,"publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"article_number":"jipb.70309","scopus_import":"1","corr_author":"1","author":[{"full_name":"Smoljan, Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana"},{"last_name":"Koutnik‐Abele","full_name":"Koutnik‐Abele, Sarah","first_name":"Sarah"},{"first_name":"Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","full_name":"Vladimirtsev, Dmitrii"},{"first_name":"Petr","full_name":"Klíma, Petr","last_name":"Klíma"},{"first_name":"Anita","full_name":"Bírošíková, Anita","last_name":"Bírošíková"},{"full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","first_name":"Yuzhou","orcid":"0000-0003-2627-6956"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609"},{"first_name":"Maximilian","last_name":"Schuster","id":"37e65def-d415-11eb-ae59-a7b67be103db","full_name":"Schuster, Maximilian"},{"last_name":"Kurtović","full_name":"Kurtović, Katarina","first_name":"Katarina"},{"first_name":"Ulrich Z.","last_name":"Hammes","full_name":"Hammes, Ulrich Z."},{"last_name":"Petrášek","full_name":"Petrášek, Jan","first_name":"Jan"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"publication":"Journal of Integrative Plant Biology","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"external_id":{"pmid":["42271607"]},"oa_version":"Published Version","researchdata_availability":"no","_id":"22301","status":"public","publisher":"Wiley","citation":{"ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026.","chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>."},"acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","year":"2026","day":"10","das_tickbox":"0","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1111/jipb.70309","OA_type":"hybrid","PlanS_conform":"1","has_accepted_license":"1","date_published":"2026-06-10T00:00:00Z","ddc":["580"],"OA_place":"publisher","date_updated":"2026-07-13T14:26:31Z","abstract":[{"text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"file":[{"content_type":"application/pdf","date_updated":"2026-07-27T14:01:34Z","creator":"dernst","checksum":"06dfad92b1465ed614a1201b4129960a","access_level":"open_access","relation":"main_file","file_name":"2026_JourMicroscopy_Goudarzi.pdf","date_created":"2026-07-27T14:01:34Z","file_size":4625767,"success":1,"file_id":"22593"}],"intvolume":"       302","external_id":{"pmid":["42104760"]},"issue":"3","oa_version":"Published Version","researchdata_availability":"no","_id":"21883","status":"public","year":"2026","citation":{"ieee":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens, “3D printing in core facilities – Low pain, high gain,” <i>Journal of Microscopy</i>, vol. 302, no. 3. Wiley, pp. 382–395, 2026.","apa":"Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., &#38; Krens, G. (2026). 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. Wiley. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>","ama":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. 2026;302(3):382-395. doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>","ista":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3), 382–395.","short":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens, Journal of Microscopy 302 (2026) 382–395.","mla":"Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>, vol. 302, no. 3, Wiley, 2026, pp. 382–95, doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>.","chicago":"Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel, Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>."},"acknowledgement":"This work was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum University of Basel, Imaging Core Facility) for sharing the design of the adopted power meter.\r\nOpen Access funding provided by Institute of Science and Technology Austria.","publisher":"Wiley","day":"01","das_tickbox":"0","fulldoi":"https://doi.org/10.1111/jmi.70106","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["600"],"OA_type":"hybrid","has_accepted_license":"1","date_published":"2026-06-01T00:00:00Z","PlanS_conform":"1","OA_place":"publisher","date_updated":"2026-07-27T14:02:46Z","file_date_updated":"2026-07-27T14:01:34Z","abstract":[{"lang":"eng","text":"Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imaging core facilities, 3D printing enables creating customised solutions for sample holders, optical components and everyday laboratory tools that traditionally required specialised machining. By providing rapid prototyping, low-cost production and reproducibility, 3D printing facilitates innovation and efficiency in facility operations. This article provides a perspective on the possibilities, challenges, and practical aspects of implementing 3D printing within microscopy core facilities. Instead of providing technical review about 3D printing, we focus on service organisation, user engagement, resource management and community-driven repositories for design dissemination. Our aim is to share insights with those considering the implementation of 3D printing as a service for developing add-on components to ease the operation of different aspects of the machine-park driven services and those who are managing advanced instrumentation within research groups."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2026-05-17T22:02:11Z","supplementarymaterial":"no","article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","volume":302,"language":[{"iso":"eng"}],"department":[{"_id":"Bio"}],"publication_status":"published","page":"382-395","month":"06","doi":"10.1111/jmi.70106","type":"journal_article","pmid":1,"publication_identifier":{"issn":["0022-2720"],"eissn":["1365-2818"]},"oa":1,"title":"3D printing in core facilities – Low pain, high gain","scopus_import":"1","corr_author":"1","author":[{"full_name":"Goudarzi, Mohammad","last_name":"Goudarzi","id":"3384113A-F248-11E8-B48F-1D18A9856A87","first_name":"Mohammad"},{"full_name":"Schuster, Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db","last_name":"Schuster","first_name":"Maximilian"},{"last_name":"Milberger","full_name":"Milberger, Arthur","first_name":"Arthur"},{"first_name":"Manuel","full_name":"Gunkel, Manuel","last_name":"Gunkel"},{"first_name":"Stefan","full_name":"Terjung, Stefan","last_name":"Terjung"},{"full_name":"Krens, Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","orcid":"0000-0003-4761-5996"}],"publication":"Journal of Microscopy"},{"article_number":"6499","pmid":1,"publication_identifier":{"eissn":["2041-1723"]},"oa":1,"title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","doi":"10.1038/s41467-026-72366-z","type":"journal_article","publication":"Nature Communications","author":[{"full_name":"Naik, Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","last_name":"Naik","first_name":"Suyash","orcid":"0000-0001-8421-5508"},{"full_name":"Keta, Yann-Edwin","last_name":"Keta","first_name":"Yann-Edwin"},{"full_name":"Pranjic-Ferscha, Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Henkes, Silke","last_name":"Henkes","first_name":"Silke"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"corr_author":"1","scopus_import":"1","project":[{"grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46","name":"Keratins in epithelial tissue spreading"},{"grant_number":"W1250-B20","name":"Nano-Analytics of Cellular Systems","_id":"252C3B08-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"quality_controlled":"1","related_material":{"record":[{"status":"public","id":"20465","relation":"earlier_version"}]},"date_created":"2026-07-29T09:10:35Z","supplementarymaterial":"yes","article_processing_charge":"Yes","month":"07","volume":17,"language":[{"iso":"eng"}],"department":[{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EdHa"}],"publication_status":"published","OA_place":"publisher","ddc":["570"],"OA_type":"gold","has_accepted_license":"1","PlanS_conform":"1","date_published":"2026-07-17T00:00:00Z","article_type":"original","fulldoi":"https://doi.org/10.1038/s41467-026-72366-z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2026-07-29T10:27:25Z","abstract":[{"text":"For tissues to spread, they must deform while staying intact. How spreading tissues balance flexibility with integrity is not yet well understood. Here, we show that keratin intermediate filaments adapt tissue mechanical resilience to the stresses arising in epithelial tissues during spreading. By analyzing the expansion of the enveloping cell layer (EVL) over the yolk cell in zebrafish embryos in vivo, we find that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, to prevent tissue rupture. Further, keratins are required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the forces pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production and EVL mechanical resilience, ensuring uniform and robust tissue spreading.","lang":"eng"}],"date_updated":"2026-07-29T10:33:31Z","external_id":{"pmid":["42143048"]},"file":[{"file_id":"22609","success":1,"file_size":15363936,"date_created":"2026-07-29T10:27:25Z","file_name":"2026_NatureComm_Naik.pdf","relation":"main_file","checksum":"f26d96e180c1d034d9c9c8f57c3c258b","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2026-07-29T10:27:25Z"}],"intvolume":"        17","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"dataavailabilitystatement":"The authors declare that the minimum dataset that is necessary to\r\ninterpret, verify, and extend the research in this article is included in\r\nthe supplementary information, the source data, and the archived data\r\nrepository (https://doi.org/10.15479/AT-ISTA-21137). This is also available\r\non GitHub at https://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data. Source data are provided\r\nwith this paper. The framework to develop the vertex models used in this paper are\r\navailable online on GitHub and archived in the source data provided.\r\nCustom scripts used for analysis of imaging and simulation data are\r\nprovided along with data files for all panels in the source data for this\r\nmanuscript on GitHub and in data repo (https://doi.org/10.15479/ATISTA-\r\n21137). Framework for the vertex model is available at https://\r\ngithub.com/yketta/cells. Code for analysis is available on GitHub\r\nhttps://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data.","das_tickbox":"1","day":"17","acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","citation":{"ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (2026). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. 2026. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. 17, 6499.","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>, vol. 17, 6499, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>.","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, Nature Communications 17 (2026).","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>."},"year":"2026","publisher":"Springer Nature","status":"public","_id":"22608","researchdata_availability":"yes","oa_version":"Published Version"},{"type":"journal_article","doi":"10.1038/s41586-026-10679-1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-026-10679-1"}],"title":"Cortical development dynamics across autism spectrum disorder mouse models","oa":1,"pmid":1,"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"author":[{"last_name":"Schwarz","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Lena A","first_name":"Lena A"},{"orcid":"0000-0002-9033-9096","first_name":"Christoph","full_name":"Dotter, Christoph","last_name":"Dotter","id":"4C66542E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sergey","last_name":"Isaev","full_name":"Isaev, Sergey"},{"full_name":"Lisi, Michela","last_name":"Lisi","id":"39383c1b-d3eb-11ef-8d6c-c8cdf4e10c8c","first_name":"Michela"},{"first_name":"Daniel","last_name":"Malzl","full_name":"Malzl, Daniel"},{"first_name":"Christoph","full_name":"Büschl, Christoph","last_name":"Büschl","id":"2a8c054c-0913-11ee-9159-f8ef515809ed"},{"last_name":"Ladstätter","full_name":"Ladstätter, Sabrina","first_name":"Sabrina"},{"first_name":"Bárbara","last_name":"Oliveira","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","full_name":"Oliveira, Bárbara"},{"last_name":"Barel","id":"8959927b-2236-11ed-bd6e-ea83d94ade0e","full_name":"Barel, Matteo","first_name":"Matteo"},{"orcid":"0000-0003-1843-3173","first_name":"Bernadette","full_name":"Basilico, Bernadette","last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425"},{"orcid":"0000-0003-4252-1608","first_name":"Chaitanya","full_name":"Chintaluri, Chaitanya","id":"BA06AFEE-A4BA-11EA-AE5C-14673DDC885E","last_name":"Chintaluri"},{"first_name":"Sarah","last_name":"Gorkiewicz","id":"f141a35d-15a9-11ec-9fb2-fef6becc7b6f","full_name":"Gorkiewicz, Sarah"},{"id":"3384113A-F248-11E8-B48F-1D18A9856A87","last_name":"Goudarzi","full_name":"Goudarzi, Mohammad","first_name":"Mohammad"},{"first_name":"Tereza","last_name":"Belinova","id":"0bf89b6a-d28b-11eb-8bd6-f43768e4d368","full_name":"Belinova, Tereza"},{"first_name":"Stephan","last_name":"Reichl","full_name":"Reichl, Stephan"},{"id":"dd6d52f2-c50d-11eb-9548-bcf0ff82b344","last_name":"Sendžikaitė","full_name":"Sendžikaitė, Gintarė","first_name":"Gintarė"},{"orcid":"0000-0002-2479-2669","first_name":"Satish","last_name":"Arcot Jayaram","id":"b0bbee33-09f7-11eb-909c-8b358058d28a","full_name":"Arcot Jayaram, Satish"},{"first_name":"Peter","orcid":"0000-0002-3509-1948","last_name":"Koppensteiner","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","full_name":"Koppensteiner, Peter"},{"first_name":"Christoph M","orcid":"0000-0003-1216-9105","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"first_name":"Tim P","orcid":"0000-0003-3295-6181","full_name":"Vogels, Tim P","last_name":"Vogels","id":"CB6FF8D2-008F-11EA-8E08-2637E6697425"},{"last_name":"Menche","full_name":"Menche, Jörg","first_name":"Jörg"},{"first_name":"Igor","full_name":"Adameyko, Igor","last_name":"Adameyko"},{"id":"0095641e-7eb7-11f1-8665-aec51a2ab5e0","last_name":"Kharchenko","full_name":"Kharchenko, Peter Vasili","first_name":"Peter Vasili"},{"full_name":"Bock, Christoph","last_name":"Bock","first_name":"Christoph"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","full_name":"Novarino, Gaia","first_name":"Gaia","orcid":"0000-0002-7673-7178"}],"publication":"Nature","scopus_import":"1","corr_author":"1","quality_controlled":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/patterns-in-genetic-chaos/","description":"News on ISTA website","relation":"press_release"}]},"project":[{"_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders","grant_number":"101044865"},{"name":"Critical windows and reversibility of ASD associated with mutations in chromatin remodelers","_id":"9B91375C-BA93-11EA-9121-9846C619BF3A","grant_number":"707964"},{"call_identifier":"FWF","name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232"},{"name":"Neurobiology of anxiety in autism spectrum disorders","_id":"ebb38b5d-77a9-11ec-83b8-a42e08120a88","grant_number":"FG1803 49015"}],"article_processing_charge":"Yes (via OA deal)","date_created":"2026-07-13T09:47:21Z","supplementarymaterial":"yes","month":"06","publication_status":"epub_ahead","language":[{"iso":"eng"}],"department":[{"_id":"AnKi"},{"_id":"GaNo"},{"_id":"TiVo"},{"_id":"ScienComp"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"PreCl"}],"OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1038/s41586-026-10679-1","article_type":"original","has_accepted_license":"1","PlanS_conform":"1","OA_type":"hybrid","date_published":"2026-06-17T00:00:00Z","ddc":["570"],"abstract":[{"lang":"eng","text":"Despite the functional diversity of over 100 causal genes1,2,3, phenotypic convergence across models may reveal common neurobiological processes in autism spectrum disorder (ASD). Here we profiled 251 samples from 11 monogenic mouse models of ASD using single-nucleus multi-omic sequencing across three developmental stages, both sexes and two brain regions. Despite genetic heterogeneity, ASD-linked mutations converged on perturbations of the radial glial cell lineage. These alterations reflect a transient developmental delay rather than lasting lineage misspecification and resolve by postnatal stages. Molecularly, the largest transcriptional differences emerged in neurons at early postnatal stages. These changes included downregulation of synaptic and ion channel-related genes, consistent with homeostatic adaptation or delayed maturation. Network analysis showed molecular convergence across models within each developmental stage, suggesting that diverse mutations linked to ASD impinge on common, stage-specific processes. Convergence becomes less pronounced by postnatal day 14, highlighting the dynamic nature of ASD-associated changes. Cross-genotype heterogeneity is superimposed on stage-specific effects. Electrophysiology corroborated this pattern: mutants generally showed altered neuronal excitability and synaptic properties with model-specific nuances. Our study also highlighted sex-specific gene expression alterations, with female mice often displaying larger effect sizes than male mice. Together, our findings provide a comprehensive view of developmental cellular and molecular dynamics across models of ASD."}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-08-04T09:29:55Z","external_id":{"pmid":["42310454"]},"dataavailabilitystatement":"Single-nucleus multiomics data are available from the Gene Expression Omnibus (GSE328363). The mm10 reference genome was used for the alignment (refdata-cellranger-arc-mm10-2020-A-2.0.0, obtained from https://cf.10xgenomics.com/supp/cell-arc/refdata-cellranger-arc-mm10-2020-A-2.0.0.tar.gz). Single-cell data can be accessed and visualized through a CELLxGENE database (https://adameykolab.hifo.meduniwien.ac.at/cellxgene_public/filecrawl/.2026_Nature_Schwarz). Source data are provided with this paper. Scripts and analyses that support the main findings of this study are accessible in a GitHub repository (https://git.ista.ac.at/research-sofware/mouseome).","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publisher":"Springer Nature","year":"2026","acknowledgement":"We thank F. Freeman, V. Voronin and M. Ladron de Guevara for technical assistance; A. Stichelberger and S. Liegenfeld for the management of our animal colony; M. Schunn, C. Gold and the Preclinical Facility team for technical assistance; C. Jansen and the Scientific Computing Facility for bioinformatics support and technical assistance; the Biomedical Sequencing Facility at CeMM for assistance with next-generation sequencing; and J. Lin and T. Krausgruber in the laboratory of C. Bock for support with flow cytometry; J. Kirchner for illustrating the multi-omics approach depicted in Fig. 1; and all members of the laboratory of G.N. for their support and discussions. This study was supported by the Scientific Service Units of ISTA through resources provided by the Imaging & Optics Facility and the Laboratory Support Facility. Bulk RNA-seq was performed by the Next Generation Sequencing Facility at Vienna BioCenter Core Facilities, member of the Vienna BioCenter. This work was supported by a European Research Council Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by the Austrian Science Fund (PE1028W1232 and PR1028FG1803) to G.N. Open access funding provided by Institute of Science and Technology (IST Austria).","citation":{"short":"L.A. Schwarz, C. Dotter, S. Isaev, M. Lisi, D. Malzl, C. Büschl, S. Ladstätter, B. Oliveira, M. Barel, B. Basilico, C. Chintaluri, S. Gorkiewicz, M. Goudarzi, T. Belinova, S. Reichl, G. Sendžikaitė, S. Arcot Jayaram, P. Koppensteiner, C.M. Sommer, T.P. Vogels, J. Menche, I. Adameyko, P.V. Kharchenko, C. Bock, G. Novarino, Nature (2026).","mla":"Schwarz, Lena A., et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” <i>Nature</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41586-026-10679-1\">10.1038/s41586-026-10679-1</a>.","chicago":"Schwarz, Lena A, Christoph Dotter, Sergey Isaev, Michela Lisi, Daniel Malzl, Christoph Büschl, Sabrina Ladstätter, et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-026-10679-1\">https://doi.org/10.1038/s41586-026-10679-1</a>.","ieee":"L. A. Schwarz <i>et al.</i>, “Cortical development dynamics across autism spectrum disorder mouse models,” <i>Nature</i>. Springer Nature, 2026.","apa":"Schwarz, L. A., Dotter, C., Isaev, S., Lisi, M., Malzl, D., Büschl, C., … Novarino, G. (2026). Cortical development dynamics across autism spectrum disorder mouse models. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-026-10679-1\">https://doi.org/10.1038/s41586-026-10679-1</a>","ista":"Schwarz LA, Dotter C, Isaev S, Lisi M, Malzl D, Büschl C, Ladstätter S, Oliveira B, Barel M, Basilico B, Chintaluri C, Gorkiewicz S, Goudarzi M, Belinova T, Reichl S, Sendžikaitė G, Arcot Jayaram S, Koppensteiner P, Sommer CM, Vogels TP, Menche J, Adameyko I, Kharchenko PV, Bock C, Novarino G. 2026. Cortical development dynamics across autism spectrum disorder mouse models. Nature.","ama":"Schwarz LA, Dotter C, Isaev S, et al. Cortical development dynamics across autism spectrum disorder mouse models. <i>Nature</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41586-026-10679-1\">10.1038/s41586-026-10679-1</a>"},"day":"17","oa_version":"Published Version","status":"public","_id":"22295","researchdata_availability":"yes"},{"external_id":{"pmid":["42681228"]},"file":[{"file_id":"22938","success":1,"file_name":"2026_MIMB_Vijatovic.pdf","file_size":1004024,"date_created":"2026-09-17T07:28:53Z","relation":"main_file","checksum":"e7a075a9ee1b91d5824f8f2b9b1f3c9b","access_level":"open_access","content_type":"application/pdf","date_updated":"2026-09-17T07:28:53Z","creator":"dernst"}],"intvolume":"      3049","day":"02","das_tickbox":"0","year":"2026","acknowledgement":"This work was supported by a Horizon Europe ERC Starting Grant\r\nNumber 101041551 (L.B.S., D.V., S.P.), Special Research Program\r\n(SFB) of the Austrian Science Fund (FWF) F7814-B (L.B.S., S.P.),\r\nAustrian Science Fund (FWF) 10.55776/COE16 (L.B.S.), FTI\r\nStrategy Lower Austria Dissertation Grant Number FT121-D-046\r\n(D.V.), and Austrian Academy of Sciences DOC Fellowship 27229\r\n(S.P.). We also thank the Imaging & Optics and Scientific Computing Facilities at ISTA for their support in developing the quantification pipeline.","citation":{"apa":"Vijatovic, D., Papadopoulos, S., Dalla Vecchia, M., &#38; Sweeney, L. B. (2026). Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In C. W. Beck (Ed.), <i>Xenopus</i> (Vol. 3049, pp. 245–259). Springer. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">https://doi.org/10.1007/978-1-0716-5360-9_11</a>","ieee":"D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, and L. B. Sweeney, “Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections,” in <i>Xenopus</i>, vol. 3049, C. W. Beck, Ed. Springer, 2026, pp. 245–259.","ista":"Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. 2026.Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In: Xenopus. Methods in Molecular Biology, vol. 3049, 245–259.","ama":"Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In: Beck CW, ed. <i>Xenopus</i>. Vol 3049. MIMB. Springer; 2026:245-259. doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">10.1007/978-1-0716-5360-9_11</a>","short":"D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, L.B. Sweeney, in:, C.W. Beck (Ed.), Xenopus, Springer, 2026, pp. 245–259.","mla":"Vijatovic, David, et al. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” <i>Xenopus</i>, edited by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 245–59, doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">10.1007/978-1-0716-5360-9_11</a>.","chicago":"Vijatovic, David, Stavros Papadopoulos, Marco Dalla Vecchia, and Lora B. Sweeney. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” In <i>Xenopus</i>, edited by Caroline W. Beck, 3049:245–59. MIMB. Springer, 2026. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">https://doi.org/10.1007/978-1-0716-5360-9_11</a>."},"publisher":"Springer","status":"public","_id":"22925","researchdata_availability":"no","oa_version":"Published Version","editor":[{"first_name":"Caroline W.","last_name":"Beck","full_name":"Beck, Caroline W."}],"OA_place":"publisher","ddc":["570"],"date_published":"2026-09-02T00:00:00Z","has_accepted_license":"1","OA_type":"hybrid","fulldoi":"https://doi.org/10.1007/978-1-0716-5360-9_11","keyword":["mRNA FISH","Hybridization chain reaction","In situ hybridization","Fluorescence microscopy"],"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2026-09-17T07:28:53Z","abstract":[{"lang":"eng","text":"Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated image analysis pipeline that enables per-cell quantification of probe expression and co-localization. This method provides a robust and quantitative approach for visualizing gene expression patterns in situ."}],"date_updated":"2026-09-17T07:31:16Z","alternative_title":["Methods in Molecular Biology"],"project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"},{"_id":"cf428362-b037-11f1-b015-8277a8a2f63d","name":"Neuronal circuits in health and disease (Sweeney)","grant_number":"COE16"},{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046"},{"name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis","_id":"907b765e-16d5-11f0-9cad-fef108a945b1","grant_number":"27229"}],"quality_controlled":"1","date_created":"2026-09-13T22:01:55Z","supplementarymaterial":"no","article_processing_charge":"No","month":"09","series_title":"MIMB","page":"245-259","volume":3049,"language":[{"iso":"eng"}],"department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"IAS"}],"publication_status":"published","pmid":1,"publication_identifier":{"eissn":["1940-6029"]},"oa":1,"title":"Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections","doi":"10.1007/978-1-0716-5360-9_11","type":"book_chapter","publication":"Xenopus","author":[{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic","full_name":"Vijatovic, David","first_name":"David","orcid":"0000-0002-5494-0941"},{"first_name":"Stavros","last_name":"Papadopoulos","id":"40606b92-f128-11eb-9611-bf66a98cfa5c","full_name":"Papadopoulos, Stavros"},{"last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","full_name":"Dalla Vecchia, Marco","first_name":"Marco"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"corr_author":"1","scopus_import":"1"},{"DOAJ_listed":"1","OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1038/s42003-026-10581-6","article_type":"original","date_published":"2026-10-08T00:00:00Z","OA_type":"gold","has_accepted_license":"1","PlanS_conform":"1","ddc":["570"],"file_date_updated":"2026-10-19T06:19:39Z","abstract":[{"text":"Presenilin-1 (PS1) is an endoplasmic reticulum protein, most known for its role in pathogenesis of familial Alzheimer’s Disease (AD). PS1 has been attributed roles in intracellular calcium homeostasis in the brain, as well as in the pancreatic beta cells, where it has been shown to be fundamental for the initial phase of glucose-induced insulin secretion. To gain mechanistic insight into beta cell autonomous function of PS1, we have examined various beta cell models and found that PS1 controls glycolytic flux of pancreatic beta cells by regulating sub-mitochondrial Ca2+ homeostasis. Transient knock down of PS1 yielded a glycolytic bottleneck at the step catalyzed by glyceraldehyde-3-phosphate dehydrogenase, resulting in defective glucose responsiveness. Moreover, we show that PS1 is needed for the preservation of beta cell identity, likely due to its role in glycolysis. Finally, we show that mouse islets from an in vivo model of AD mimic the defective glucose responsiveness seen in beta cells with transient PS1 knock down, while the identity loss and whole-body glucose homeostasis are not severely impacted, suggesting an adaptive response. Thus, the current work highlights beta cell autonomous role of PS1 and adds a layer of complexity in its impact on whole body metabolism.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-19T06:23:26Z","intvolume":"         9","file":[{"access_level":"open_access","checksum":"b90e60a625fcac8ef5d9ff968792d675","relation":"main_file","content_type":"application/pdf","date_updated":"2026-10-19T06:19:39Z","creator":"dernst","success":1,"file_id":"23161","file_name":"2026_CommunicationsBiology_Koshenov.pdf","date_created":"2026-10-19T06:19:39Z","file_size":1819866}],"external_id":{"pmid":["42387039"]},"dataavailabilitystatement":"The data supporting the findings of this research can be obtained from the authors upon a reasonable request; please refer to the author’s contributions for details regarding specific datasets. Numerical source data underlying graphs in this article can be found in Supplementary data file. Supplementary Figs. and figure legends are included in the Supplementary file. The code used in this study is available from the authors on reasonable request.","publisher":"Springer Nature","citation":{"ama":"Koshenov Z, Postic S, Schoiswohl G, et al. Presenilin-1 controls glycolysis and identity of pancreatic beta cells. <i>Communications Biology</i>. 2026;9. doi:<a href=\"https://doi.org/10.1038/s42003-026-10581-6\">10.1038/s42003-026-10581-6</a>","ista":"Koshenov Z, Postic S, Schoiswohl G, Van Amsterdam S, Hois-Zelinka V, Oflaz FE, Rost R, Tiapko O, Gottschalk B, Hirtl M, Bachkoenig OG, Koshenova A, Erdogan YC, Sagintayev A, Krnjic A, Pfabe JU, Sarikas S, Hochreiter B, Gindlhuber J, Schittmayer M, Tadic J, Ehall B, Madeo F, Madl T, Malli R, Birner-Gruenberger R, Pieber T, Eisenberg T, Slak Rupnik M, Graier WF. 2026. Presenilin-1 controls glycolysis and identity of pancreatic beta cells. Communications Biology. 9, 1315.","apa":"Koshenov, Z., Postic, S., Schoiswohl, G., Van Amsterdam, S., Hois-Zelinka, V., Oflaz, F. E., … Graier, W. F. (2026). Presenilin-1 controls glycolysis and identity of pancreatic beta cells. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-026-10581-6\">https://doi.org/10.1038/s42003-026-10581-6</a>","ieee":"Z. Koshenov <i>et al.</i>, “Presenilin-1 controls glycolysis and identity of pancreatic beta cells,” <i>Communications Biology</i>, vol. 9. Springer Nature, 2026.","chicago":"Koshenov, Zhanat, Sandra Postic, Gabriela Schoiswohl, Savina Van Amsterdam, Victoria Hois-Zelinka, Furkan E. Oflaz, Rene Rost, et al. “Presenilin-1 Controls Glycolysis and Identity of Pancreatic Beta Cells.” <i>Communications Biology</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s42003-026-10581-6\">https://doi.org/10.1038/s42003-026-10581-6</a>.","short":"Z. Koshenov, S. Postic, G. Schoiswohl, S. Van Amsterdam, V. Hois-Zelinka, F.E. Oflaz, R. Rost, O. Tiapko, B. Gottschalk, M. Hirtl, O.G. Bachkoenig, A. Koshenova, Y.C. Erdogan, A. Sagintayev, A. Krnjic, J.U. Pfabe, S. Sarikas, B. Hochreiter, J. Gindlhuber, M. Schittmayer, J. Tadic, B. Ehall, F. Madeo, T. Madl, R. Malli, R. Birner-Gruenberger, T. Pieber, T. Eisenberg, M. Slak Rupnik, W.F. Graier, Communications Biology 9 (2026).","mla":"Koshenov, Zhanat, et al. “Presenilin-1 Controls Glycolysis and Identity of Pancreatic Beta Cells.” <i>Communications Biology</i>, vol. 9, 1315, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s42003-026-10581-6\">10.1038/s42003-026-10581-6</a>."},"year":"2026","acknowledgement":"We appreciate the technical assistance from Anna Schreilechner. We are grateful for support by all members of the animal facility team at IMB, University of Graz, as well as K. Zierler for assistance with animal licenses.  F.M, T.E., T.M., T.P., and W.F.G. are grateful to the Austrian Science Fund (FWF) for the excellence cluster 10.55776/COE14 (MetAGE), which was additionally supported by Land Styria, Stadt Graz, University of Graz and Medical University of Graz. This research was further supported by the FWF via the DKplus program 10.55776/W1226 (DK-MCD) to F.M., T.M., R.B.G., and W.F.G., 10.55776/P28529, 10.55776/I3716 (to R.M.), 10.55776/P28854, 10.55776/I3792, 10.55776/DOC130 (to T.M.), and 10.55776/I4319 (to. M.S.R.); the Austrian Research Promotion Agency (FFG) grants 864690 and 870454; the Integrative Metabolism Research Center Graz; the Austrian Infrastructure Program 2016/2017 (to T.M.), and 10.55776/COE7, 10.55776/COE17, 10.55776/FG12 and 10.55776/F73 (to R.B.G.); the Styrian Government; the City of Graz; and BioTechMed-Graz (flagship project) (to T.M.); the MEFO Graz (to W.F.G.). F.E.O., J.G. and Z.K. were doctoral fellows in the doctoral program Metabolic and Cardiovascular Disease (MCD) (FWF, 10.55776/W 1226) at the Medical University of Graz. S.v.A. and A.S. are thesis students in the excellence cluster 10.55776/COE14 (MetAGE, W.F.G.). M.H. was a fellow of the Molecular Medicine (MolMed) doctoral program at the Medical University of Graz supported by MEFO Graz. The SIM equipment is part of the Nikon Center of Excellence, Graz, and is supported by the Austrian infrastructure program 2013/ 2014, Nikon Austria Inc., and BioTechMed (to W.F.G.). Our thanks go to Shimadzu Austria for supporting this research through instrument access given in the Metabolomics and Bioprocess Analytics laboratory (TU Wien) (R.B.G.). For open access purposes, the authors have applied a CC BY public copyright license to any author-accepted manuscript version arising from this submission.” We thank the Medical University of Graz for financial support.","das_tickbox":"1","day":"08","oa_version":"Published Version","_id":"23148","researchdata_availability":"upon request","status":"public","article_number":"1315","type":"journal_article","doi":"10.1038/s42003-026-10581-6","oa":1,"title":"Presenilin-1 controls glycolysis and identity of pancreatic beta cells","publication_identifier":{"eissn":["2399-3642"]},"pmid":1,"author":[{"full_name":"Koshenov, Zhanat","last_name":"Koshenov","first_name":"Zhanat"},{"first_name":"Sandra","last_name":"Postic","full_name":"Postic, Sandra"},{"first_name":"Gabriela","last_name":"Schoiswohl","full_name":"Schoiswohl, Gabriela"},{"first_name":"Savina","full_name":"Van Amsterdam, Savina","last_name":"Van Amsterdam"},{"first_name":"Victoria","full_name":"Hois-Zelinka, Victoria","last_name":"Hois-Zelinka"},{"full_name":"Oflaz, Furkan E.","last_name":"Oflaz","first_name":"Furkan E."},{"last_name":"Rost","full_name":"Rost, Rene","first_name":"Rene"},{"first_name":"Oleksandra","last_name":"Tiapko","full_name":"Tiapko, Oleksandra"},{"first_name":"Benjamin","last_name":"Gottschalk","full_name":"Gottschalk, Benjamin"},{"full_name":"Hirtl, Martin","last_name":"Hirtl","first_name":"Martin"},{"first_name":"Olaf G.","full_name":"Bachkoenig, Olaf G.","last_name":"Bachkoenig"},{"last_name":"Koshenova","full_name":"Koshenova, Aigerim","first_name":"Aigerim"},{"last_name":"Erdogan","full_name":"Erdogan, Yusuf C.","first_name":"Yusuf C."},{"first_name":"Adlet","last_name":"Sagintayev","full_name":"Sagintayev, Adlet"},{"first_name":"Anita","full_name":"Krnjic, Anita","last_name":"Krnjic"},{"first_name":"Johannes U.","last_name":"Pfabe","full_name":"Pfabe, Johannes U."},{"full_name":"Sarikas, Srdjan","id":"35F0286E-F248-11E8-B48F-1D18A9856A87","last_name":"Sarikas","first_name":"Srdjan"},{"first_name":"Bernhard","last_name":"Hochreiter","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","full_name":"Hochreiter, Bernhard"},{"last_name":"Gindlhuber","full_name":"Gindlhuber, Juergen","first_name":"Juergen"},{"first_name":"Matthias","full_name":"Schittmayer, Matthias","last_name":"Schittmayer"},{"full_name":"Tadic, Jelena","last_name":"Tadic","first_name":"Jelena"},{"full_name":"Ehall, Barbara","last_name":"Ehall","first_name":"Barbara"},{"first_name":"Frank","last_name":"Madeo","full_name":"Madeo, Frank"},{"first_name":"Tobias","last_name":"Madl","full_name":"Madl, Tobias"},{"last_name":"Malli","full_name":"Malli, Roland","first_name":"Roland"},{"first_name":"Ruth","last_name":"Birner-Gruenberger","full_name":"Birner-Gruenberger, Ruth"},{"first_name":"Thomas","last_name":"Pieber","full_name":"Pieber, Thomas"},{"first_name":"Tobias","last_name":"Eisenberg","full_name":"Eisenberg, Tobias"},{"full_name":"Slak Rupnik, Marjan","last_name":"Slak Rupnik","first_name":"Marjan"},{"full_name":"Graier, Wolfgang F.","last_name":"Graier","first_name":"Wolfgang F."}],"publication":"Communications Biology","scopus_import":"1","quality_controlled":"1","article_processing_charge":"Yes","supplementarymaterial":"yes","date_created":"2026-10-18T22:01:50Z","month":"10","publication_status":"published","language":[{"iso":"eng"}],"volume":9,"department":[{"_id":"FISS"}]},{"type":"journal_article","doi":"10.1038/s41586-026-11063-9","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-026-11063-9"}],"oa":1,"title":"Dynamic protrusions mediate crawling motility in Asgard archaea","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"pmid":1,"author":[{"orcid":"0000-0001-9198-2182 ","first_name":"Philipp","full_name":"Radler, Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","last_name":"Radler"},{"first_name":"Tobias","last_name":"Viehboeck","full_name":"Viehboeck, Tobias"},{"first_name":"Zhen-Hao","full_name":"Luo, Zhen-Hao","last_name":"Luo"},{"first_name":"Nevena","full_name":"Maslać, Nevena","last_name":"Maslać"},{"last_name":"Schmidt","full_name":"Schmidt, Katharina","first_name":"Katharina"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"first_name":"Masaru K.","full_name":"Nobu, Masaru K.","last_name":"Nobu"},{"full_name":"Bulgheresi, Silvia","last_name":"Bulgheresi","first_name":"Silvia"},{"first_name":"Theresia E. B.","full_name":"Stradal, Theresia E. B.","last_name":"Stradal"},{"first_name":"Klemens","full_name":"Rottner, Klemens","last_name":"Rottner"},{"last_name":"Imachi","full_name":"Imachi, Hiroyuki","first_name":"Hiroyuki"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K"},{"last_name":"Schleper","full_name":"Schleper, Christa","first_name":"Christa"}],"publication":"Nature","scopus_import":"1","quality_controlled":"1","project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"article_processing_charge":"Yes (via OA deal)","date_created":"2026-10-19T09:26:53Z","supplementarymaterial":"yes","month":"09","publication_status":"epub_ahead","language":[{"iso":"eng"}],"department":[{"_id":"MiSi"},{"_id":"Bio"}],"OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1038/s41586-026-11063-9","article_type":"original","date_published":"2026-09-30T00:00:00Z","OA_type":"hybrid","has_accepted_license":"1","PlanS_conform":"1","ddc":["570"],"abstract":[{"text":"Crawling motility is a hallmark of eukaryotic cells and requires a dynamic actin cytoskeleton, regulated adhesion and spatially organized signalling pathways1,2. Asgard archaea, which are considered the closest known prokaryotic relatives of eukaryotes, potentially encode these functions within their large set of ‘eukaryotic signature proteins’3,4,5,6. The few cultivated members show a complex cell morphology, consisting of a central cell body from which several protrusions extend, filled with an actin-based cytoskeleton7,8. Here, live-cell microscopy of two organisms of the Lokiarchaea and Hodarchaea lineages7,9 showed that they dynamically and greatly change their cell shape on a minute time scale and grow and retract their extensive protrusions with a speed of 1.5–4.8 µm min−1, respectively. After adhering to a glass surface, cells use their protrusions to undergo active crawling motion. In the presence of selected actin inhibitors, however, the observed dynamics were arrested, suggesting a central role of actin in these processes. The observed cellular plasticity and motility are unique features among prokaryotes and might have been crucial for the emergence of the first eukaryotic cells that are thought to have formed through the association of a member of the Asgard archaea and an α-proteobacterium, the ancestor of mitochondria.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-19T09:40:50Z","external_id":{"pmid":["42816618"]},"dataavailabilitystatement":"The plots in this manuscript were created using Jupyter Notebook; the source data for all figures have been provided in data source tables. Microscopy raw data for supplementary videos, the Jupyter notebooks to generate the plots and the raw files for the structural phylogenetic analysis are available at https://doi.org/10.6084/m9.figshare.30676367. The remaining raw microscopy data generated for this manuscript are available from https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD3928. The mass spectrometry proteomics data from the Co-IP experiments have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository79 and are available at https://www.ebi.ac.uk/pride/archive/projects/PXD068820. The mass spectrometry proteomics data of the proteome analysis is available at https://www.ebi.ac.uk/pride/archive/projects/PXD074767. Source data are provided with this paper.","acknowledged_ssus":[{"_id":"Bio"}],"publisher":"Springer Nature","citation":{"ista":"Radler P, Viehboeck T, Luo Z-H, Maslać N, Schmidt K, Hauschild R, Nobu MK, Bulgheresi S, Stradal TEB, Rottner K, Imachi H, Sixt MK, Schleper C. 2026. Dynamic protrusions mediate crawling motility in Asgard archaea. Nature.","ama":"Radler P, Viehboeck T, Luo Z-H, et al. Dynamic protrusions mediate crawling motility in Asgard archaea. <i>Nature</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41586-026-11063-9\">10.1038/s41586-026-11063-9</a>","ieee":"P. Radler <i>et al.</i>, “Dynamic protrusions mediate crawling motility in Asgard archaea,” <i>Nature</i>. Springer Nature, 2026.","apa":"Radler, P., Viehboeck, T., Luo, Z.-H., Maslać, N., Schmidt, K., Hauschild, R., … Schleper, C. (2026). Dynamic protrusions mediate crawling motility in Asgard archaea. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-026-11063-9\">https://doi.org/10.1038/s41586-026-11063-9</a>","chicago":"Radler, Philipp, Tobias Viehboeck, Zhen-Hao Luo, Nevena Maslać, Katharina Schmidt, Robert Hauschild, Masaru K. Nobu, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-026-11063-9\">https://doi.org/10.1038/s41586-026-11063-9</a>.","mla":"Radler, Philipp, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” <i>Nature</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41586-026-11063-9\">10.1038/s41586-026-11063-9</a>.","short":"P. Radler, T. Viehboeck, Z.-H. Luo, N. Maslać, K. Schmidt, R. Hauschild, M.K. Nobu, S. Bulgheresi, T.E.B. Stradal, K. Rottner, H. Imachi, M.K. Sixt, C. Schleper, Nature (2026)."},"acknowledgement":"This work would not have been possible without the technical support in cultivation from K. Hager, F. Baraket, A. Malits and S. Harrer. We thank the Imaging and Optics Facility (IOF) at ISTA for their help in setting up the anaerobic environment during imaging and the Cell Imaging & Ultrastructure Research (CIUS) facility at the University of Vienna for their support. Proteomics analyses were performed by the Mass Spectrometry Facility at Max Perutz Labs using the VBCF instrument pool. We also thank P. Klahn (University of Gothenburg, Sweden), M. Stadler and members of his department (HZI Braunschweig) as well as R. Cox (LUH Hannover) for providing different cytochalasans and P. Bieling (King’s College London) for discussion. Most of this work was funded by the Austrian Science Fund (FWF) with grant DOI 10.55776/Z437 (available at https://doi.org/10.55776/Z437) and further supported by an emerging field grant with grant DOI 10.55776/EFP25 (available at https://doi.org/10.55776/EFP25) to C.S. For open access purposes, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission. This work was further supported by a European Research Council grant ERC-SyG (101071793) to M.S., by the German Research Council through the CytoLabs consortium (Research Unit 5170 to K.R. and T.E.B.S.), by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research 22H04985 to H.I. and M.N. Furthermore, R.H. was supported by CZI grant DAF2020-225401 and a grant (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF. Open access funding provided by University of Vienna.","year":"2026","das_tickbox":"1","day":"30","oa_version":"Published Version","_id":"23171","status":"public","researchdata_availability":"yes"},{"acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","citation":{"ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Cell Press, p. 356–371.e12, 2026.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Cell Press, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>."},"year":"2026","publisher":"Cell Press","day":"11","das_tickbox":"1","oa_version":"Published Version","_id":"20859","researchdata_availability":"upon request","status":"public","file":[{"file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","file_size":12342817,"date_created":"2026-07-23T06:26:25Z","file_id":"22388","success":1,"content_type":"application/pdf","date_updated":"2026-07-23T06:26:25Z","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"52fd52d2d19a4514f8fcc1b40f420ca2"}],"intvolume":"        61","external_id":{"pmid":["41192429"]},"issue":"2","acknowledged_ssus":[{"_id":"NanoFab"}],"dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","abstract":[{"lang":"eng","text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo."}],"file_date_updated":"2026-07-23T06:26:25Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T09:01:14Z","OA_place":"publisher","article_type":"original","fulldoi":"https://doi.org/10.1016/j.devcel.2025.10.006","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"ddc":["570"],"has_accepted_license":"1","OA_type":"hybrid","date_published":"2026-02-11T00:00:00Z","PlanS_conform":"1","month":"02","language":[{"iso":"eng"}],"volume":61,"department":[{"_id":"Bio"},{"_id":"NanoFab"}],"publication_status":"published","page":"356-371.e12","quality_controlled":"1","date_created":"2025-12-28T23:01:27Z","supplementarymaterial":"yes","article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"Iván","last_name":"Company-Garrido","full_name":"Company-Garrido, Iván"},{"last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto","first_name":"Alberto"},{"first_name":"Mariona","last_name":"Colomer-Rosell","full_name":"Colomer-Rosell, Mariona"},{"first_name":"Bernard","full_name":"Ciraulo, Bernard","last_name":"Ciraulo"},{"last_name":"Molkenbur","full_name":"Molkenbur, Ronja","first_name":"Ronja"},{"first_name":"Peter","full_name":"Lanzerstorfer, Peter","last_name":"Lanzerstorfer"},{"full_name":"Pezzano, Fabio","last_name":"Pezzano","first_name":"Fabio"},{"full_name":"Agazzi, Costanza","last_name":"Agazzi","first_name":"Costanza"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Jain","full_name":"Jain, Saumey","first_name":"Saumey"},{"last_name":"Jacques","full_name":"Jacques, Jeroen M.","first_name":"Jeroen M."},{"full_name":"Venturini, Valeria","last_name":"Venturini","first_name":"Valeria"},{"first_name":"Christian","last_name":"Knapp","full_name":"Knapp, Christian"},{"last_name":"Xie","full_name":"Xie, Yufei","first_name":"Yufei"},{"last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609"},{"first_name":"Julian","last_name":"Weghuber","full_name":"Weghuber, Julian"},{"first_name":"Marcel","full_name":"Schaaf, Marcel","last_name":"Schaaf"},{"last_name":"Quidant","full_name":"Quidant, Romain","first_name":"Romain"},{"last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva","first_name":"Eva","orcid":"0000-0001-6165-5738"},{"first_name":"Jaime","last_name":"Ortega Arroyo","full_name":"Ortega Arroyo, Jaime"},{"orcid":"0000-0003-4088-8633","first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena"},{"orcid":"0000-0002-2670-2217","first_name":"Stefan","last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan"}],"publication":"Developmental Cell","scopus_import":"1","doi":"10.1016/j.devcel.2025.10.006","type":"journal_article","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"pmid":1,"oa":1,"title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses"},{"DOAJ_listed":"1","OA_place":"publisher","date_published":"2026-04-28T00:00:00Z","PlanS_conform":"1","OA_type":"gold","has_accepted_license":"1","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.celrep.2026.117227","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2026-05-04T12:20:10Z","abstract":[{"text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution.","lang":"eng"}],"date_updated":"2026-10-20T09:04:23Z","issue":"4","external_id":{"pmid":["41964955 "]},"intvolume":"        45","file":[{"file_name":"2026_CellReports_Vijatovic.pdf","file_size":14925958,"date_created":"2026-05-04T12:20:10Z","file_id":"21795","success":1,"date_updated":"2026-05-04T12:20:10Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"day":"28","publisher":"Cell Press","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","year":"2026","citation":{"short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>.","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Cell Press, 2026.","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>"},"status":"public","_id":"21746","oa_version":"Published Version","article_number":"117227","oa":1,"title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"pmid":1,"type":"journal_article","doi":"10.1016/j.celrep.2026.117227","publication":"Cell Reports","author":[{"first_name":"David","orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Toma, Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","last_name":"Toma","first_name":"Florina Alexandra "},{"full_name":"Ignatyev, Y","last_name":"Ignatyev","first_name":"Y"},{"first_name":"Zoe P","orcid":"0009-0008-0158-4032","full_name":"Harrington, Zoe P","last_name":"Harrington","id":"a8144562-32c9-11ee-b5ce-d9800628bda2"},{"last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","first_name":"Christoph M","orcid":"0000-0003-1216-9105"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Matthijs Geert","last_name":"Smits","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","full_name":"Smits, Matthijs Geert"},{"first_name":"Marco","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","last_name":"Dalla Vecchia","full_name":"Dalla Vecchia, Marco"},{"first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J.","last_name":"Trevisan"},{"full_name":"Chapman, Phillip","last_name":"Chapman","first_name":"Phillip"},{"first_name":"Mara","full_name":"Julseth, Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"first_name":"Susan","last_name":"Brenner-Morton","full_name":"Brenner-Morton, Susan"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"first_name":"Jeremy S.","last_name":"Dasen","full_name":"Dasen, Jeremy S."},{"last_name":"Bikoff","full_name":"Bikoff, Jay B.","first_name":"Jay B."},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger"}],"corr_author":"1","scopus_import":"1","project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"},{"grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"_id":"cf428362-b037-11f1-b015-8277a8a2f63d","name":"Neuronal circuits in health and disease (Sweeney)","grant_number":"COE16"}],"quality_controlled":"1","related_material":{"record":[{"status":"public","id":"22667","relation":"dissertation_contains"}]},"article_processing_charge":"Yes","date_created":"2026-04-19T22:07:43Z","month":"04","publication_status":"published","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"language":[{"iso":"eng"}],"volume":45},{"date_updated":"2026-01-05T11:49:54Z","scopus_import":"1","publication":"Transcriptomics in Atherosclerosis","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"RNA sequencing (RNA-seq) methodologies have evolved rapidly, offering powerful tools to study gene expression, transcriptome dynamics, and molecular mechanisms in various biological contexts. However, the complexity of these approaches poses challenges in data interpretation, sensitivity, and applicability. This chapter provides a comprehensive overview of RNA-seq methodologies, highlighting their advantages, limitations, and applications, particularly in cardiovascular research. Bulk RNA sequencing enables high-throughput gene expression profiling but lacks the resolution to capture cellular heterogeneity and spatial context. Direct RNA sequencing preserves native RNA modifications, offering insights into post-transcriptional regulation, though it remains technically challenging. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) bridge these gaps by resolving transcriptomic complexity at the cellular level and within tissue architecture, providing crucial insights into disease mechanisms such as atherosclerosis. By summarizing the strengths and limitations of these methodologies, this chapter aims to guide researchers in selecting the most suitable transcriptomic approach for their studies, ultimately advancing precision medicine and biomarker discovery in cardiovascular disease."}],"author":[{"full_name":"Stopa, Victoria","last_name":"Stopa","first_name":"Victoria"},{"first_name":"Miron","full_name":"Sopić, Miron","last_name":"Sopić"},{"first_name":"Guanliang","last_name":"Li","full_name":"Li, Guanliang"},{"first_name":"Judith","last_name":"Sluimer","full_name":"Sluimer, Judith"},{"full_name":"Basílio, José","last_name":"Basílio","first_name":"José"},{"full_name":"van der Laan, Sander W.","last_name":"van der Laan","first_name":"Sander W."},{"first_name":"David P.","last_name":"Kreil","full_name":"Kreil, David P."},{"last_name":"Devaux","full_name":"Devaux, Yvan","first_name":"Yvan"},{"last_name":"Hochreiter","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","full_name":"Hochreiter, Bernhard","first_name":"Bernhard"}],"publication_identifier":{"isbn":["9780443330643"]},"date_published":"2025-10-24T00:00:00Z","title":"Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond","OA_type":"closed access","fulldoi":"https://doi.org/10.1016/b978-0-443-33064-3.00016-5","doi":"10.1016/b978-0-443-33064-3.00016-5","type":"book_chapter","page":"131-172","_id":"20870","status":"public","department":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"publication_status":"published","oa_version":"None","editor":[{"full_name":"Devaux, Yvan","last_name":"Devaux","first_name":"Yvan"},{"last_name":"Sopic","full_name":"Sopic, Miron","first_name":"Miron"}],"day":"24","month":"10","year":"2025","citation":{"apa":"Stopa, V., Sopić, M., Li, G., Sluimer, J., Basílio, J., van der Laan, S. W., … Hochreiter, B. (2025). Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In Y. Devaux &#38; M. Sopic (Eds.), <i>Transcriptomics in Atherosclerosis</i> (pp. 131–172). Elsevier. <a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">https://doi.org/10.1016/b978-0-443-33064-3.00016-5</a>","ieee":"V. Stopa <i>et al.</i>, “Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond,” in <i>Transcriptomics in Atherosclerosis</i>, Y. Devaux and M. Sopic, Eds. Elsevier, 2025, pp. 131–172.","ama":"Stopa V, Sopić M, Li G, et al. Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Devaux Y, Sopic M, eds. <i>Transcriptomics in Atherosclerosis</i>. Elsevier; 2025:131-172. doi:<a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">10.1016/b978-0-443-33064-3.00016-5</a>","ista":"Stopa V, Sopić M, Li G, Sluimer J, Basílio J, van der Laan SW, Kreil DP, Devaux Y, Hochreiter B. 2025.Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Transcriptomics in Atherosclerosis. , 131–172.","short":"V. Stopa, M. Sopić, G. Li, J. Sluimer, J. Basílio, S.W. van der Laan, D.P. Kreil, Y. Devaux, B. Hochreiter, in:, Y. Devaux, M. Sopic (Eds.), Transcriptomics in Atherosclerosis, Elsevier, 2025, pp. 131–172.","mla":"Stopa, Victoria, et al. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” <i>Transcriptomics in Atherosclerosis</i>, edited by Yvan Devaux and Miron Sopic, Elsevier, 2025, pp. 131–72, doi:<a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">10.1016/b978-0-443-33064-3.00016-5</a>.","chicago":"Stopa, Victoria, Miron Sopić, Guanliang Li, Judith Sluimer, José Basílio, Sander W. van der Laan, David P. Kreil, Yvan Devaux, and Bernhard Hochreiter. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” In <i>Transcriptomics in Atherosclerosis</i>, edited by Yvan Devaux and Miron Sopic, 131–72. Elsevier, 2025. <a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">https://doi.org/10.1016/b978-0-443-33064-3.00016-5</a>."},"publisher":"Elsevier","date_created":"2025-12-29T12:16:22Z","article_processing_charge":"No","quality_controlled":"1"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the non-canonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g. control of costly-to-induce multi-drug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multi-drug resistance is crucial for effective public health measures.","lang":"eng"}],"author":[{"first_name":"Kirti","orcid":"0000-0002-3809-0449","full_name":"Jain, Kirti","last_name":"Jain","id":"330F0278-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"full_name":"Bochkareva, Olga","id":"C4558D3C-6102-11E9-A62E-F418E6697425","last_name":"Bochkareva","first_name":"Olga","orcid":"0000-0003-1006-6639"},{"id":"68E56E44-62B0-11EA-B963-444F3DDC885E","last_name":"Römhild","full_name":"Römhild, Roderich","first_name":"Roderich","orcid":"0000-0001-9480-5261"},{"orcid":"0000-0002-6699-1455","first_name":"Gašper","full_name":"Tkačik, Gašper","last_name":"Tkačik","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","first_name":"Calin C","orcid":"0000-0001-6220-2052"}],"file_date_updated":"2025-03-05T07:39:38Z","corr_author":"1","date_updated":"2026-05-20T08:33:07Z","OA_place":"repository","ddc":["570"],"date_published":"2025-03-04T00:00:00Z","OA_type":"gold","title":"Data for \"Pulsatile basal gene expression as a fitness determinant in bacteria\"","has_accepted_license":"1","oa":1,"doi":"10.15479/AT:ISTA:19294","fulldoi":"https://doi.org/10.15479/AT:ISTA:19294","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"research_data","day":"04","month":"03","year":"2025","citation":{"short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).","mla":"Jain, Kirti, et al. <i>Data for “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.”</i> Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>.","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>.","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’” Institute of Science and Technology Austria, 2025.","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” 2025. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>."},"publisher":"Institute of Science and Technology Austria","status":"public","_id":"19294","department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"oa_version":"Published 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basal gene expression as a fitness determinant in bacteria","pmid":1,"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"type":"journal_article","doi":"10.1073/pnas.2413709122","publication":"Proceedings of the National Academy of Sciences","author":[{"orcid":"0000-0002-3809-0449","first_name":"Kirti","full_name":"Jain, Kirti","id":"330F0278-F248-11E8-B48F-1D18A9856A87","last_name":"Jain"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"first_name":"Olga","orcid":"0000-0003-1006-6639","full_name":"Bochkareva, Olga","last_name":"Bochkareva","id":"C4558D3C-6102-11E9-A62E-F418E6697425"},{"first_name":"Roderich","orcid":"0000-0001-9480-5261","full_name":"Römhild, Roderich","last_name":"Römhild","id":"68E56E44-62B0-11EA-B963-444F3DDC885E"},{"first_name":"Gašper","orcid":"0000-0002-6699-1455","last_name":"Tkačik","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkačik, Gašper"},{"last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","first_name":"Calin C"}],"corr_author":"1","scopus_import":"1","project":[{"name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","grant_number":"CZI01"},{"name":"Non-canonical antibiotic interactions","_id":"bd6f94d1-d553-11ed-ba76-ae9f07250f74","grant_number":"E219"},{"_id":"34e076d6-11ca-11ed-8bc3-aec76c41a181","name":"Evolutionary analysis of gene regulation","grant_number":"I05127"}],"related_material":{"link":[{"url":"https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/","relation":"press_release","description":"News on ISTA website"}],"record":[{"status":"public","id":"19294","relation":"research_data"}]},"quality_controlled":"1","article_processing_charge":"Yes (in subscription journal)","APC_amount":"5949 EUR","date_created":"2025-04-27T22:02:13Z","month":"04","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"volume":122,"OA_place":"publisher","has_accepted_license":"1","OA_type":"hybrid","date_published":"2025-04-15T00:00:00Z","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1073/pnas.2413709122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the noncanonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures."}],"file_date_updated":"2025-06-24T07:27:43Z","date_updated":"2026-05-20T08:33:08Z","issue":"15","external_id":{"pmid":["40193613"],"isi":["001471235200001"]},"intvolume":"       122","file":[{"file_id":"19888","success":1,"file_size":2949523,"date_created":"2025-06-24T07:27:43Z","file_name":"2025_PNAS_Jain.pdf","relation":"main_file","checksum":"115a687f40009660eb4b38b4f6559d41","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2025-06-24T07:27:43Z"}],"acknowledged_ssus":[{"_id":"Bio"}],"isi":1,"day":"15","publisher":"National Academy of Sciences","citation":{"chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>.","mla":"Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15, e2413709122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings of the National Academy of Sciences 122 (2025).","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(15). doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 122(15), e2413709122.","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Pulsatile basal gene expression as a fitness determinant in bacteria,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15. National Academy of Sciences, 2025.","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>"},"year":"2025","acknowledgement":"K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions on the manuscript, all other members from the Guet laboratory for valuable feedback, R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic Technology India for allowing time off to address the revisions. K.J. acknowledges Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi), O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.","status":"public","_id":"19626","oa_version":"Published Version"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules that grow radially and exert forces on cargos. At the same time, mechanical stresses from the microenvironment and cellular shape changes compress and bend microtubules. Yet, centrosomes are membraneless organelles, raising the question of how centrosomes withstand mechanical forces. Here, we discover that centrosomes can deform and even fracture. We reveal that centrosomes experience deformations during navigational pathfinding within motile cells. Coherence of the centrosome is maintained by Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes cellular function by generating coexisting microtubule organizing centers that compete during path navigation and thereby cause cellular entanglement in the microenvironment. Our findings show that cells actively maintain the integrity of the centrosome to withstand mechanical forces. These results suggest that centrosome stability preservation is fundamental, given that almost all cells in multicellular organisms experience forces."}],"file_date_updated":"2025-05-12T07:46:10Z","date_updated":"2025-09-30T12:26:21Z","DOAJ_listed":"1","OA_place":"publisher","has_accepted_license":"1","OA_type":"gold","date_published":"2025-04-25T00:00:00Z","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1126/sciadv.adx4047","article_type":"original","isi":1,"day":"25","publisher":"AAAS","year":"2025","acknowledgement":"We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids; M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids; M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts, Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support; M. Benjamin Braun for critical reading of the manuscript; and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.); German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (J.R.); Medical & Clinician Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048 (D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag 457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.); Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments (E.K.); and CZI grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (R.H.).","citation":{"chicago":"Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>. AAAS, 2025. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>.","mla":"Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>, vol. 11, no. 17, eadx4047, AAAS, 2025, doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>.","short":"M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P. Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N. Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub, F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).","ista":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P, Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 11(17), eadx4047.","ama":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. 2025;11(17). doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>","ieee":"M. T. Schmitt <i>et al.</i>, “Protecting centrosomes from fracturing enables efficient cell navigation,” <i>Science Advances</i>, vol. 11, no. 17. AAAS, 2025.","apa":"Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh, S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>"},"status":"public","_id":"19663","oa_version":"Published Version","issue":"17","external_id":{"pmid":["40279414"],"isi":["001476113400016"]},"intvolume":"        11","file":[{"file_name":"2025_ScienceAdvance_Schmitt.pdf","date_created":"2025-05-12T07:46:10Z","file_size":2707050,"file_id":"19679","success":1,"content_type":"application/pdf","date_updated":"2025-05-12T07:46:10Z","creator":"dernst","relation":"main_file","checksum":"e8ba22922fa5b23ccfcce8865f57226c","access_level":"open_access"}],"publication":"Science Advances","author":[{"full_name":"Schmitt, Madeleine T.","last_name":"Schmitt","first_name":"Madeleine T."},{"first_name":"Janina","full_name":"Kroll, Janina","last_name":"Kroll"},{"full_name":"Ruiz-Fernandez, Mauricio J.A.","last_name":"Ruiz-Fernandez","first_name":"Mauricio J.A."},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"first_name":"Shaunak","full_name":"Ghosh, Shaunak","last_name":"Ghosh"},{"full_name":"Kameritsch, Petra","last_name":"Kameritsch","first_name":"Petra"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609"},{"full_name":"Schmid, Johanna","last_name":"Schmid","first_name":"Johanna"},{"last_name":"Stefanowski","full_name":"Stefanowski, Kasia","first_name":"Kasia"},{"first_name":"Andreas W.","full_name":"Thomae, Andreas W.","last_name":"Thomae"},{"last_name":"Cheng","full_name":"Cheng, Jingyuan","first_name":"Jingyuan"},{"first_name":"Gamze Naz","full_name":"Öztan, Gamze Naz","last_name":"Öztan"},{"last_name":"Konopka","full_name":"Konopka, Peter","first_name":"Peter"},{"first_name":"Germán Camargo","last_name":"Ortega","full_name":"Ortega, Germán Camargo"},{"last_name":"Penz","full_name":"Penz, Thomas","first_name":"Thomas"},{"first_name":"Luisa","full_name":"Bach, Luisa","last_name":"Bach"},{"last_name":"Baumjohann","full_name":"Baumjohann, Dirk","first_name":"Dirk"},{"last_name":"Bock","full_name":"Bock, Christoph","first_name":"Christoph"},{"last_name":"Straub","full_name":"Straub, Tobias","first_name":"Tobias"},{"last_name":"Meissner","full_name":"Meissner, Felix","first_name":"Felix"},{"last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva","orcid":"0000-0001-6165-5738","first_name":"Eva"},{"full_name":"Renkawitz, Jörg","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2856-3369","first_name":"Jörg"}],"scopus_import":"1","article_number":"eadx4047","title":"Protecting centrosomes from fracturing enables efficient cell navigation","oa":1,"pmid":1,"publication_identifier":{"eissn":["2375-2548"]},"type":"journal_article","doi":"10.1126/sciadv.adx4047","month":"04","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"Bio"},{"_id":"NanoFab"}],"volume":11,"project":[{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"quality_controlled":"1","article_processing_charge":"Yes","date_created":"2025-05-11T22:02:38Z"},{"file":[{"file_name":"2025_NatureImmunology_ReisRodrigues.pdf","date_created":"2025-07-31T08:00:33Z","file_size":13514646,"file_id":"20096","success":1,"date_updated":"2025-07-31T08:00:33Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","checksum":"0c725123dca7797c682609bff2c4c5ac","access_level":"open_access"}],"intvolume":"        26","external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","year":"2025","citation":{"ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>."},"publisher":"Springer Nature","day":"01","isi":1,"oa_version":"Published Version","_id":"20082","status":"public","OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s41590-025-02211-w","article_type":"letter_note","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"OA_type":"hybrid","has_accepted_license":"1","date_published":"2025-08-01T00:00:00Z","PlanS_conform":"1","file_date_updated":"2025-07-31T08:00:33Z","abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-04-28T13:26:50Z","quality_controlled":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/bench-pressing-cells/","description":"News on ISTA website","relation":"press_release"}],"record":[{"id":"20149","relation":"dissertation_contains","status":"public"}]},"project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"},{"_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration","grant_number":"944-2020"}],"date_created":"2025-07-27T22:01:26Z","article_processing_charge":"Yes (via OA deal)","month":"08","language":[{"iso":"eng"}],"volume":26,"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"publication_status":"published","page":"1258–1266","doi":"10.1038/s41590-025-02211-w","type":"journal_article","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"pmid":1,"oa":1,"title":"Migrating immune cells globally coordinate protrusive forces","author":[{"full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","orcid":"0000-0003-1681-508X","first_name":"Patricia"},{"orcid":"0000-0001-6406-524X","first_name":"Mario","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","full_name":"Avellaneda Sarrió, Mario"},{"last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87","full_name":"Canigova, Nikola","first_name":"Nikola","orcid":"0000-0002-8518-5926"},{"orcid":"0000-0001-6120-3723","first_name":"Florian R","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner"},{"orcid":"0000-0001-7829-3518","first_name":"Kari","full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","id":"368EE576-F248-11E8-B48F-1D18A9856A87"},{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","last_name":"Riedl","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","first_name":"Michael"},{"last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","first_name":"Ingrid"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert"},{"first_name":"Yoshinori","full_name":"Fukui, Yoshinori","last_name":"Fukui"},{"full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba","orcid":"0000-0002-1009-9652"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt"}],"publication":"Nature Immunology","scopus_import":"1","corr_author":"1"},{"status":"public","_id":"17468","oa_version":"Published Version","isi":1,"day":"16","publisher":"Springer Nature","acknowledgement":"S.A.F. thanks the Institute of Science and Technology Austria (ISTA) for the support. The Scientific Service Units of ISTA supported this research through resources provided by the Imaging and Optics Facility, the Lab Support Facility, the Miba Machine Shop and Scientific Computing. This research was partly funded by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open access purposes, the author has applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. R.H. acknowledges funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989). H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life and R. Jethwa for proofreading. Open access funding was provided by ISTA.","year":"2025","citation":{"mla":"Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>, vol. 646, no. 8085, Springer Nature, 2025, pp. 601–605, doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>.","short":"S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025) 601–605.","chicago":"Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>.","ieee":"S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics control singlet and triplet oxygen evolving from superoxide,” <i>Nature</i>, vol. 646, no. 8085. Springer Nature, pp. 601–605, 2025.","apa":"Mondal, S., Nguyen, H. T. K., Hauschild, R., &#38; Freunberger, S. A. (2025). Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>","ista":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605.","ama":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. 2025;646(8085):601–605. doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>"},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"issue":"8085","external_id":{"isi":["001586378900001"],"pmid":["41044415"]},"intvolume":"       646","file":[{"date_updated":"2025-10-20T10:26:13Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"b507ddd23df0388aa65d04dc9b00fe3d","file_name":"2025_Nature_Mondal.pdf","date_created":"2025-10-20T10:26:13Z","file_size":3809247,"file_id":"20500","success":1}],"date_updated":"2026-09-16T06:53:54Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Oxygen redox chemistry is central to life1 and many human-made technologies, such as in energy storage2,3,4. The large energy gain from oxygen redox reactions is often connected with the occurrence of harmful reactive oxygen species3,5,6. Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which may evolve from superoxide. However, the factors determining the formation of singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown. Here we report that the release of triplet or singlet oxygen is governed by individual Marcus normal and inverted region behaviour. We found that as the driving force for the reaction increases, the initially dominant evolution of triplet oxygen slows down, and singlet oxygen evolution becomes predominant with higher maximum kinetics. This behaviour also applies to the widely observed superoxide disproportionation, in which one superoxide is oxidized by another, in both non-aqueous and aqueous systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet oxygen yields governed by these conditions are relevant, for example, in batteries or cellular organelles in which superoxide forms. Our findings suggest ways to understand and control spin states and kinetics in oxygen redox chemistry, with implications for fields, including life sciences, pure chemistry and energy storage."}],"file_date_updated":"2025-10-20T10:26:13Z","PlanS_conform":"1","has_accepted_license":"1","date_published":"2025-10-16T00:00:00Z","OA_type":"hybrid","ddc":["540"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1038/s41586-025-09587-7","article_type":"original","OA_place":"publisher","page":"601–605","publication_status":"published","department":[{"_id":"StFr"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"volume":646,"month":"10","article_processing_charge":"Yes (via OA deal)","date_created":"2024-08-29T10:40:23Z","project":[{"grant_number":"P37169","name":"Singlet oxygen in non-aqueous oxygen redox chemistry","_id":"8df062be-16d5-11f0-9cad-f559b6612c7e"},{"grant_number":"CZI01","name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"grant_number":"COE05","name":"Materials for Energy Conversion and Storage (Freunberger)","_id":"5eaf4378-b033-11f1-b276-f928018a46c1"}],"related_material":{"link":[{"url":"https://ista.ac.at/en/news/taming-the-bad-oxygen/","description":"News on ISTA website","relation":"press_release"}]},"quality_controlled":"1","corr_author":"1","scopus_import":"1","publication":"Nature","author":[{"last_name":"Mondal","id":"d25d21ef-dc8d-11ea-abe3-ec4576307f48","full_name":"Mondal, Soumyadip","first_name":"Soumyadip"},{"first_name":"Huyen T.K.","full_name":"Nguyen, Huyen T.K.","last_name":"Nguyen"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"first_name":"Stefan Alexander","orcid":"0000-0003-2902-5319","full_name":"Freunberger, Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","last_name":"Freunberger"}],"title":"Marcus kinetics control singlet and triplet oxygen evolving from superoxide","oa":1,"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"pmid":1,"type":"journal_article","doi":"10.1038/s41586-025-09587-7"},{"month":"06","publication_status":"published","language":[{"iso":"eng"}],"volume":642,"department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"GaNo"}],"page":"398-410","quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"research_data","id":"18697"},{"status":"public","id":"18677","relation":"earlier_version"}],"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/"}]},"project":[{"grant_number":"26137","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"665385"},{"name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders","_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","grant_number":"101044865"},{"grant_number":"W1232-B24","name":"Molecular Drug Targets","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"article_processing_charge":"Yes (via OA deal)","date_created":"2025-05-18T22:02:51Z","author":[{"orcid":"0000-0002-7667-6854","first_name":"Mojtaba","full_name":"Tavakoli, Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","last_name":"Tavakoli"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","last_name":"Lyudchik","full_name":"Lyudchik, Julia","first_name":"Julia"},{"first_name":"Michał","full_name":"Januszewski, Michał","last_name":"Januszewski"},{"last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","full_name":"Vistunou, Vitali","first_name":"Vitali"},{"first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie"},{"first_name":"Jakob","orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","last_name":"Vorlaufer"},{"orcid":"0000-0003-1216-9105","first_name":"Christoph M","full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Caroline","full_name":"Kreuzinger, Caroline","last_name":"Kreuzinger","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Bárbara","last_name":"Oliveira","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","full_name":"Oliveira, Bárbara"},{"first_name":"Alban","last_name":"Cenameri","id":"9ac8f577-2357-11eb-997a-e566c5550886","full_name":"Cenameri, Alban"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","full_name":"Novarino, Gaia","first_name":"Gaia","orcid":"0000-0002-7673-7178"},{"first_name":"Viren","full_name":"Jain, Viren","last_name":"Jain"},{"last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","first_name":"Johann G"}],"publication":"Nature","scopus_import":"1","corr_author":"1","type":"journal_article","doi":"10.1038/s41586-025-08985-1","title":"Light-microscopy-based connectomic reconstruction of mammalian brain tissue","oa":1,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"pmid":1,"publisher":"Springer Nature","citation":{"chicago":"Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>.","mla":"Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>, vol. 642, Springer Nature, 2025, pp. 398–410, doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>.","short":"M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas, J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino, V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.","ama":"Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. 2025;642:398-410. doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>","ista":"Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 642, 398–410.","ieee":"M. Tavakoli <i>et al.</i>, “Light-microscopy-based connectomic reconstruction of mammalian brain tissue,” <i>Nature</i>, vol. 642. Springer Nature, pp. 398–410, 2025.","apa":"Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas, N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>"},"year":"2025","acknowledgement":"We thank S. Dorkenwald and P. Li for critical reading of the manuscript, S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open access funding provided by Institute of Science and Technology (IST Austria).","isi":1,"day":"12","oa_version":"Published Version","status":"public","_id":"19704","intvolume":"       642","file":[{"checksum":"ebc99d7108e728f46db0a009292675ef","access_level":"open_access","relation":"main_file","date_updated":"2025-07-03T06:55:20Z","content_type":"application/pdf","creator":"dernst","success":1,"file_id":"19959","file_name":"2025_Nature_Tavakoli.pdf","file_size":133201290,"date_created":"2025-07-03T06:55:20Z"}],"external_id":{"isi":["001483477000001"],"pmid":["40335689"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"PreCl"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"file_date_updated":"2025-07-03T06:55:20Z","ec_funded":1,"abstract":[{"lang":"eng","text":"The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction by light microscopy has been out of reach, owing to limitations in resolution, contrast and volumetric imaging capability. Here we describe light-microscopy-based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning-based segmentation and analysis of connectivity, thereby directly incorporating molecular information into synapse-level reconstructions of brain tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological experiments in a readily adoptable manner."}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-10-02T09:59:44Z","OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1038/s41586-025-08985-1","article_type":"original","PlanS_conform":"1","date_published":"2025-06-12T00:00:00Z","has_accepted_license":"1","OA_type":"hybrid","ddc":["570"]},{"quality_controlled":"1","project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"},{"_id":"269CD5C4-B435-11E9-9278-68D0E5697425","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","grant_number":"ALTF 1159-2018"}],"date_created":"2025-03-16T23:01:24Z","article_processing_charge":"Yes","month":"03","volume":44,"language":[{"iso":"eng"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"publication_status":"published","article_number":"115387","doi":"10.1016/j.celrep.2025.115387","type":"journal_article","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"pmid":1,"title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","oa":1,"author":[{"first_name":"Ste","orcid":"0000-0001-9970-7804","full_name":"Tavano, Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","last_name":"Tavano"},{"first_name":"David","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","last_name":"Brückner","full_name":"Brückner, David"},{"first_name":"Saren","orcid":"0000-0003-1671-393X","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","full_name":"Tasciyan, Saren"},{"first_name":"Xin","full_name":"Tong, Xin","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","last_name":"Tong"},{"full_name":"Kardos, Roland","last_name":"Kardos","id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland"},{"last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","full_name":"Schauer, Alexandra","first_name":"Alexandra","orcid":"0000-0001-7659-9142"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"publication":"Cell Reports","scopus_import":"1","corr_author":"1","file":[{"access_level":"open_access","checksum":"57e05dd1598c807af0afdb32cec039d3","relation":"main_file","content_type":"application/pdf","date_updated":"2025-03-17T10:26:54Z","creator":"dernst","success":1,"file_id":"19413","file_name":"2025_CellReports_Tavano.pdf","date_created":"2025-03-17T10:26:54Z","file_size":9067797}],"intvolume":"        44","external_id":{"isi":["001443652700001"],"pmid":["40057955"]},"issue":"3","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"citation":{"mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>.","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Cell Press, 2025.","ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387."},"acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","year":"2025","publisher":"Cell Press","day":"25","isi":1,"oa_version":"Published Version","status":"public","_id":"19404","OA_place":"publisher","DOAJ_listed":"1","fulldoi":"https://doi.org/10.1016/j.celrep.2025.115387","article_type":"original","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"ddc":["570"],"has_accepted_license":"1","date_published":"2025-03-25T00:00:00Z","OA_type":"gold","file_date_updated":"2025-03-17T10:26:54Z","abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:20:45Z"},{"doi":"10.1101/2024.09.20.614050","fulldoi":"https://doi.org/10.1101/2024.09.20.614050","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.09.20.614050"}],"type":"preprint","oa":1,"title":"Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis","OA_type":"green","date_published":"2024-09-27T00:00:00Z","OA_place":"repository","corr_author":"1","date_updated":"2025-05-14T11:40:13Z","abstract":[{"lang":"eng","text":"Vertebrates exhibit a wide range of motor behaviors, ranging from swimming to complex limb-based movements. Here we take advantage of frog metamorphosis, which captures a swim-to-limb-based movement transformation during the development of a single organism, to explore changes in the underlying spinal circuits. We find that the tadpole spinal cord contains small and largely homogeneous populations of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages. These neuronal populations only modestly increase in number and subtype heterogeneity with the emergence of free swimming. In contrast, during frog metamorphosis and the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron number and transcriptional heterogeneity, culminating in cohorts of neurons that exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively, results in severe but selective deficits in tail and limb function. Our work thus demonstrates that neural diversity scales exponentially with increasing behavioral complexity and illustrates striking evolutionary conservation in the molecular organization and function of motor circuits across species."}],"author":[{"last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58","full_name":"Vijatovic, David","first_name":"David"},{"first_name":"Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","last_name":"Toma","full_name":"Toma, Florina Alexandra "},{"first_name":"Zoe P","orcid":"0009-0008-0158-4032","last_name":"Harrington","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","full_name":"Harrington, Zoe P"},{"first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"full_name":"Trevisan, Alexandra J.","last_name":"Trevisan","first_name":"Alexandra J."},{"first_name":"Phillip","full_name":"Chapman, Phillip","last_name":"Chapman"},{"first_name":"Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","full_name":"Julseth, Mara"},{"full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton","first_name":"Susan"},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"first_name":"Jeremy S.","full_name":"Dasen, Jeremy S.","last_name":"Dasen"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B.","first_name":"Jay B."},{"full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}],"publication":"bioRxiv","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-04-07T08:48:28Z","acknowledged_ssus":[{"_id":"Bio"}],"article_processing_charge":"No","project":[{"grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"department":[{"_id":"LoSw"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"language":[{"iso":"eng"}],"publication_status":"submitted","oa_version":"Preprint","_id":"19520","status":"public","acknowledgement":"We would like to thank the members of the Sweeney Lab (especially Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion, and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their scientific insight and mentorship during the conception of this project. This\r\nproject would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA). In addition, we thank our funding sources for providing the resources to do these\r\nexperiments: FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS 5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.); NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities (ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472 (Z.H.); and Project A.L.S. (S.B-M.). ","citation":{"chicago":"Vijatovic, David, Florina Alexandra  Toma, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>.","mla":"Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","short":"D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).","ista":"Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv, <a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","ama":"Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>","ieee":"D. Vijatovic <i>et al.</i>, “Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis,” <i>bioRxiv</i>. .","apa":"Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R., Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>"},"year":"2024","day":"27","month":"09"},{"date_updated":"2026-10-01T08:32:34Z","abstract":[{"lang":"eng","text":"Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play key roles in germ layer specification and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants to study the coordination of Nodal and BMP signaling for embryo patterning and morphogenesis. We show that Nodal signaling triggers explant elongation by inducing mesendodermal progenitors but also suppressing BMP signaling activity at the site of mesendoderm induction. Consistent with this, ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm intercalations, key processes during explant elongation. Translating these ex vivo observations to the intact embryo showed that, similar to explants, Nodal signaling suppresses the effect of BMP signaling on cell intercalations in the dorsal domain, thus allowing robust embryonic axis elongation. These findings suggest a dual function of Nodal signaling in embryonic axis elongation by both inducing mesendoderm and suppressing BMP effects in the dorsal portion of the mesendoderm."}],"file_date_updated":"2024-03-04T07:24:43Z","ec_funded":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1242/dev.202316","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"has_accepted_license":"1","date_published":"2024-02-01T00:00:00Z","oa_version":"Published Version","researchdata_availability":"yes","_id":"15048","status":"public","acknowledgement":"We thank Patrick Müller for sharing the chordintt250 mutant zebrafish line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro and Katherine Rogers and members of the Heisenberg lab for discussions, technical advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo for discussions. We thank the Imaging and Optics Facility as well as the Life Science facility at IST Austria for support with microscopy and fish maintenance.\r\nThis work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573 to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience and Technology Austria. ","year":"2024","citation":{"ama":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18. doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>","ista":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4), 1–18.","ieee":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>, vol. 151, no. 4. Company of Biologists, pp. 1–18, 2024.","apa":"Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J. (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>","chicago":"Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>. Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>.","short":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development 151 (2024) 1–18.","mla":"Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, Company of Biologists, 2024, pp. 1–18, doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>."},"publisher":"Company of Biologists","das_tickbox":"1","day":"01","isi":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"dataavailabilitystatement":"A custom MATLAB script for analyzing cell dispersal has been deposited in the ISTA library (doi:10.15479/AT:ISTA:14926)","file":[{"checksum":"6961ea10012bf0d266681f9628bb8f13","access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_updated":"2024-03-04T07:24:43Z","creator":"dernst","success":1,"file_id":"15050","file_name":"2024_Development_Schauer.pdf","file_size":14839986,"date_created":"2024-03-04T07:24:43Z"}],"intvolume":"       151","external_id":{"pmid":["38372390"],"isi":["001170580200001"]},"issue":"4","scopus_import":"1","corr_author":"1","author":[{"orcid":"0000-0001-7659-9142","first_name":"Alexandra","full_name":"Schauer, Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer"},{"full_name":"Pranjic-Ferscha, Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","last_name":"Pranjic-Ferscha","first_name":"Kornelija"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"publication":"Development","doi":"10.1242/dev.202316","type":"journal_article","publication_identifier":{"eissn":["1477-9129"],"issn":["0950-1991"]},"pmid":1,"title":"Robust axis elongation by Nodal-dependent restriction of BMP signaling","oa":1,"language":[{"iso":"eng"}],"department":[{"_id":"CaHe"},{"_id":"Bio"}],"volume":151,"publication_status":"published","page":"1-18","month":"02","date_created":"2024-03-03T23:00:50Z","supplementarymaterial":"yes","article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","related_material":{"record":[{"id":"14926","relation":"research_data","status":"public"}]},"project":[{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239"}]}]