---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21860'
abstract:
- lang: eng
text: Glutamate excitotoxicity is a cell death mechanism triggered by accumulation
of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex
(αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point
controlling glutamate formation and its consumption as a fuel. Hence, modulation
of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic
effects. To address this hypothesis, hippocampal neurons in primary co-culture
with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through
N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures
with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion
of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover,
NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity
and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA
receptors involved mGluR1 activation and was accompanied by enhanced neuronal
discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses
evoked by glutamate application. These results suggest that mGluR1-mediated potentiation
of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might
exacerbate glutamate excitotoxicity.
acknowledgement: The technical assistance by Tanja Wagner and Elena Lilliu is gratefully
acknowledged. This research was funded in whole or in part by the Austrian Science
Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access
funding provided by Medical University of Vienna and the Austrian Science Fund (FWF).
Deposited in PMC for immediate release.
article_number: jcs264420
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Vanessa
full_name: Goeschl, Vanessa
last_name: Goeschl
- first_name: Matej
full_name: Hotka, Matej
last_name: Hotka
- first_name: Bernhard
full_name: Hochreiter, Bernhard
id: e6cab3de-17f6-11ed-9210-c1e42e045e9d
last_name: Hochreiter
- first_name: Karlheinz
full_name: Hilber, Karlheinz
last_name: Hilber
- first_name: Stefan
full_name: Boehm, Stefan
last_name: Boehm
- first_name: Andrey V.
full_name: Kozlov, Andrey V.
last_name: Kozlov
- first_name: Helmut
full_name: Kubista, Helmut
last_name: Kubista
citation:
ama: Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex
activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA
receptors in primary cultures. Journal of Cell Science. 2026;139(8). doi:10.1242/jcs.264420
apa: Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V.,
& Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates
glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary
cultures. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.264420
chicago: Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan
Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex
Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA
Receptors in Primary Cultures.” Journal of Cell Science. The Company of
Biologists, 2026. https://doi.org/10.1242/jcs.264420.
ieee: V. Goeschl et al., “α-ketoglutarate dehydrogenase complex activity
modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors
in primary cultures,” Journal of Cell Science, vol. 139, no. 8. The Company
of Biologists, 2026.
ista: Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H.
2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures. Journal of
Cell Science. 139(8), jcs264420.
mla: Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates
Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary
Cultures.” Journal of Cell Science, vol. 139, no. 8, jcs264420, The Company
of Biologists, 2026, doi:10.1242/jcs.264420.
short: V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H.
Kubista, Journal of Cell Science 139 (2026).
date_created: 2026-05-11T10:52:27Z
date_published: 2026-04-27T00:00:00Z
date_updated: 2026-05-12T06:40:18Z
day: '27'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1242/jcs.264420
external_id:
pmid:
- '41834724'
file:
- access_level: open_access
checksum: 8db35c97588c2f6ef88c7e8d5924cf8c
content_type: application/pdf
creator: dernst
date_created: 2026-05-12T06:27:54Z
date_updated: 2026-05-12T06:27:54Z
file_id: '21861'
file_name: 2026_JourCellScience_Goeschl.pdf
file_size: 1957057
relation: main_file
success: 1
file_date_updated: 2026-05-12T06:27:54Z
fulldoi: https://doi.org/10.1242/jcs.264420
has_accepted_license: '1'
intvolume: ' 139'
issue: '8'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 139
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '22301'
abstract:
- lang: eng
text: Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth
and development in land plants, but its physiological role in chlorophyte algae
remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella
sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent
manner. Low IAA concentrations promoted growth by accelerating the onset of cell
division without affecting cell size, whereas higher concentrations inhibited
proliferation. Radiotracer assays showed that all three species take up and release
IAA across the plasma membrane through a combination of passive diffusion and
energy-dependent, saturable processes. Competition by excess unlabeled natural
and synthetic auxins further supported the presence of carrier-mediated transport
with broad substrate recognition. Phylogenetic analyses identified potential PIN-like
auxin exporters in chlorophytes and other non-plant eukaryotes, and structural
modeling supported conservation of the overall PIN fold and predicted auxin-binding
residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured
cells, and Arabidopsis thaliana did not support a role for these proteins in directional
auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic
reticulum and showed limited or no transport activity in heterologous systems.
Together, these findings indicate that auxin responsiveness and basic cellular
auxin transport predate canonical PIN-mediated directional auxin export, which
appears to be a later innovation of the streptophyte lineage.
acknowledged_ssus:
- _id: Bio
- _id: NanoFab
acknowledgement: Research in the Friml group was supported by the European Research
Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian
Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from
the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional
support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG,
German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics
Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support
with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with
microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB
CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided
by Institute of Science and Technology Austria.
article_number: jipb.70309
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Sarah
full_name: Koutnik‐Abele, Sarah
last_name: Koutnik‐Abele
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Anita
full_name: Bírošíková, Anita
last_name: Bírošíková
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Maximilian
full_name: Schuster, Maximilian
id: 37e65def-d415-11eb-ae59-a7b67be103db
last_name: Schuster
- first_name: Katarina
full_name: Kurtović, Katarina
last_name: Kurtović
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated
transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026.
doi:10.1111/jipb.70309
apa: Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A.,
Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte
algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309
chicago: Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma,
Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated
Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology.
Wiley, 2026. https://doi.org/10.1111/jipb.70309.
ieee: A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte
algae,” Journal of Integrative Plant Biology. Wiley, 2026.
ista: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y,
Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin
response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative
Plant Biology., jipb. 70309.
mla: Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte
Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026,
doi:10.1111/jipb.70309.
short: A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y.
Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml,
Journal of Integrative Plant Biology (2026).
corr_author: '1'
das_tickbox: '0'
date_created: 2026-07-13T10:44:55Z
date_published: 2026-06-10T00:00:00Z
date_updated: 2026-07-13T14:26:31Z
day: '10'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: NanoFab
- _id: Bio
doi: 10.1111/jipb.70309
external_id:
pmid:
- '42271607'
fulldoi: https://doi.org/10.1111/jipb.70309
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.70309
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Auxin response and PIN‐mediated transport in chlorophyte algae
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21883'
abstract:
- lang: eng
text: Three-dimensional (3D) printing has rapidly developed from a niche hobbyist
activity into a widely accessible and indispensable technology across multiple
scientific disciplines. Within microscopy, optical engineering laboratories and
imaging core facilities, 3D printing enables creating customised solutions for
sample holders, optical components and everyday laboratory tools that traditionally
required specialised machining. By providing rapid prototyping, low-cost production
and reproducibility, 3D printing facilitates innovation and efficiency in facility
operations. This article provides a perspective on the possibilities, challenges,
and practical aspects of implementing 3D printing within microscopy core facilities.
Instead of providing technical review about 3D printing, we focus on service organisation,
user engagement, resource management and community-driven repositories for design
dissemination. Our aim is to share insights with those considering the implementation
of 3D printing as a service for developing add-on components to ease the operation
of different aspects of the machine-park driven services and those who are managing
advanced instrumentation within research groups.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
acknowledgement: "This work was supported by the Scientific Service Units (SSU) of
Institute of Science and Technology Austria (ISTA) through resources provided by
the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert
Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities
at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing
of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum
University of Basel, Imaging Core Facility) for sharing the design of the adopted
power meter.\r\nOpen Access funding provided by Institute of Science and Technology
Austria."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mohammad
full_name: Goudarzi, Mohammad
id: 3384113A-F248-11E8-B48F-1D18A9856A87
last_name: Goudarzi
- first_name: Maximilian
full_name: Schuster, Maximilian
id: 37e65def-d415-11eb-ae59-a7b67be103db
last_name: Schuster
- first_name: Arthur
full_name: Milberger, Arthur
last_name: Milberger
- first_name: Manuel
full_name: Gunkel, Manuel
last_name: Gunkel
- first_name: Stefan
full_name: Terjung, Stefan
last_name: Terjung
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
citation:
ama: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing
in core facilities – Low pain, high gain. Journal of Microscopy. 2026;302(3):382-395.
doi:10.1111/jmi.70106
apa: Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., & Krens,
G. (2026). 3D printing in core facilities – Low pain, high gain. Journal of
Microscopy. Wiley. https://doi.org/10.1111/jmi.70106
chicago: Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel,
Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain,
High Gain.” Journal of Microscopy. Wiley, 2026. https://doi.org/10.1111/jmi.70106.
ieee: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens,
“3D printing in core facilities – Low pain, high gain,” Journal of Microscopy,
vol. 302, no. 3. Wiley, pp. 382–395, 2026.
ista: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D
printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3),
382–395.
mla: Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High
Gain.” Journal of Microscopy, vol. 302, no. 3, Wiley, 2026, pp. 382–95,
doi:10.1111/jmi.70106.
short: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens,
Journal of Microscopy 302 (2026) 382–395.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-05-17T22:02:11Z
date_published: 2026-06-01T00:00:00Z
date_updated: 2026-07-27T14:02:46Z
day: '01'
ddc:
- '600'
department:
- _id: Bio
doi: 10.1111/jmi.70106
external_id:
pmid:
- '42104760'
file:
- access_level: open_access
checksum: 06dfad92b1465ed614a1201b4129960a
content_type: application/pdf
creator: dernst
date_created: 2026-07-27T14:01:34Z
date_updated: 2026-07-27T14:01:34Z
file_id: '22593'
file_name: 2026_JourMicroscopy_Goudarzi.pdf
file_size: 4625767
relation: main_file
success: 1
file_date_updated: 2026-07-27T14:01:34Z
fulldoi: https://doi.org/10.1111/jmi.70106
has_accepted_license: '1'
intvolume: ' 302'
issue: '3'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 382-395
pmid: 1
publication: Journal of Microscopy
publication_identifier:
eissn:
- 1365-2818
issn:
- 0022-2720
publication_status: published
publisher: Wiley
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: 3D printing in core facilities – Low pain, high gain
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 302
year: '2026'
...
---
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '22608'
abstract:
- lang: eng
text: For tissues to spread, they must deform while staying intact. How spreading
tissues balance flexibility with integrity is not yet well understood. Here, we
show that keratin intermediate filaments adapt tissue mechanical resilience to
the stresses arising in epithelial tissues during spreading. By analyzing the
expansion of the enveloping cell layer (EVL) over the yolk cell in zebrafish embryos
in vivo, we find that keratin network maturation in EVL cells is promoted by stresses
building up within the spreading tissue. Through genetic interference and tissue
rheology experiments, complemented by a vertex model with mechanochemical feedback,
we demonstrate that stress-induced keratin network maturation in the EVL increases
tissue viscosity, to prevent tissue rupture. Further, keratins are required in
the yolk cell for mechanosensitive actomyosin network contraction and flow, the
forces pulling the EVL. These dual mechanosensitive functions of keratins enable
a balance between pulling force production and EVL mechanical resilience, ensuring
uniform and robust tissue spreading.
acknowledged_ssus:
- _id: Bio
- _id: ScienComp
- _id: LifeSc
- _id: EM-Fac
acknowledgement: We thank all members of the Heisenberg, Henkes, and Hannezo groups
for their support. We are also grateful to the Imaging and Optics, Scientific Computing,
Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their
technical assistance and support. Numerical simulations were performed using the
computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary
Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided
by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020
research and innovation programme (grant agreement No. 665385). This work was supported
by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to
C.-P.H.
article_number: '6499'
article_processing_charge: Yes
article_type: original
author:
- first_name: Suyash
full_name: Naik, Suyash
id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
last_name: Naik
orcid: 0000-0001-8421-5508
- first_name: Yann-Edwin
full_name: Keta, Yann-Edwin
last_name: Keta
- first_name: Kornelija
full_name: Pranjic-Ferscha, Kornelija
id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
last_name: Pranjic-Ferscha
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Silke
full_name: Henkes, Silke
last_name: Henkes
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ.
Keratins coordinate tissue spreading by balancing spreading forces with tissue
material properties. Nature Communications. 2026;17. doi:10.1038/s41467-026-72366-z
apa: Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &
Heisenberg, C.-P. J. (2026). Keratins coordinate tissue spreading by balancing
spreading forces with tissue material properties. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-026-72366-z
chicago: Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo,
Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading
by Balancing Spreading Forces with Tissue Material Properties.” Nature Communications.
Springer Nature, 2026. https://doi.org/10.1038/s41467-026-72366-z.
ieee: S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P.
J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces
with tissue material properties,” Nature Communications, vol. 17. Springer
Nature, 2026.
ista: Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ.
2026. Keratins coordinate tissue spreading by balancing spreading forces with
tissue material properties. Nature Communications. 17, 6499.
mla: Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading
Forces with Tissue Material Properties.” Nature Communications, vol. 17,
6499, Springer Nature, 2026, doi:10.1038/s41467-026-72366-z.
short: S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J.
Heisenberg, Nature Communications 17 (2026).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: "The authors declare that the minimum dataset that is necessary
to\r\ninterpret, verify, and extend the research in this article is included in\r\nthe
supplementary information, the source data, and the archived data\r\nrepository
(https://doi.org/10.15479/AT-ISTA-21137). This is also available\r\non GitHub at
https://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data.
Source data are provided\r\nwith this paper. The framework to develop the vertex
models used in this paper are\r\navailable online on GitHub and archived in the
source data provided.\r\nCustom scripts used for analysis of imaging and simulation
data are\r\nprovided along with data files for all panels in the source data for
this\r\nmanuscript on GitHub and in data repo (https://doi.org/10.15479/ATISTA-\r\n21137).
Framework for the vertex model is available at https://\r\ngithub.com/yketta/cells.
Code for analysis is available on GitHub\r\nhttps://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data."
date_created: 2026-07-29T09:10:35Z
date_published: 2026-07-17T00:00:00Z
date_updated: 2026-07-29T10:33:31Z
day: '17'
ddc:
- '570'
department:
- _id: Bio
- _id: CaHe
- _id: EdHa
doi: 10.1038/s41467-026-72366-z
external_id:
pmid:
- '42143048'
file:
- access_level: open_access
checksum: f26d96e180c1d034d9c9c8f57c3c258b
content_type: application/pdf
creator: dernst
date_created: 2026-07-29T10:27:25Z
date_updated: 2026-07-29T10:27:25Z
file_id: '22609'
file_name: 2026_NatureComm_Naik.pdf
file_size: 15363936
relation: main_file
success: 1
file_date_updated: 2026-07-29T10:27:25Z
fulldoi: https://doi.org/10.1038/s41467-026-72366-z
has_accepted_license: '1'
intvolume: ' 17'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 8f060199-16d5-11f0-9cad-f3253b266c46
grant_number: PAT 5044023
name: Keratins in epithelial tissue spreading
- _id: 252C3B08-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1250-B20
name: Nano-Analytics of Cellular Systems
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '20465'
relation: earlier_version
status: public
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Keratins coordinate tissue spreading by balancing spreading forces with tissue
material properties
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
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_id: '22295'
abstract:
- lang: eng
text: 'Despite the functional diversity of over 100 causal genes1,2,3, phenotypic
convergence across models may reveal common neurobiological processes in autism
spectrum disorder (ASD). Here we profiled 251 samples from 11 monogenic mouse
models of ASD using single-nucleus multi-omic sequencing across three developmental
stages, both sexes and two brain regions. Despite genetic heterogeneity, ASD-linked
mutations converged on perturbations of the radial glial cell lineage. These alterations
reflect a transient developmental delay rather than lasting lineage misspecification
and resolve by postnatal stages. Molecularly, the largest transcriptional differences
emerged in neurons at early postnatal stages. These changes included downregulation
of synaptic and ion channel-related genes, consistent with homeostatic adaptation
or delayed maturation. Network analysis showed molecular convergence across models
within each developmental stage, suggesting that diverse mutations linked to ASD
impinge on common, stage-specific processes. Convergence becomes less pronounced
by postnatal day 14, highlighting the dynamic nature of ASD-associated changes.
Cross-genotype heterogeneity is superimposed on stage-specific effects. Electrophysiology
corroborated this pattern: mutants generally showed altered neuronal excitability
and synaptic properties with model-specific nuances. Our study also highlighted
sex-specific gene expression alterations, with female mice often displaying larger
effect sizes than male mice. Together, our findings provide a comprehensive view
of developmental cellular and molecular dynamics across models of ASD.'
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We thank F. Freeman, V. Voronin and M. Ladron de Guevara for technical
assistance; A. Stichelberger and S. Liegenfeld for the management of our animal
colony; M. Schunn, C. Gold and the Preclinical Facility team for technical assistance;
C. Jansen and the Scientific Computing Facility for bioinformatics support and technical
assistance; the Biomedical Sequencing Facility at CeMM for assistance with next-generation
sequencing; and J. Lin and T. Krausgruber in the laboratory of C. Bock for support
with flow cytometry; J. Kirchner for illustrating the multi-omics approach depicted
in Fig. 1; and all members of the laboratory of G.N. for their support and discussions.
This study was supported by the Scientific Service Units of ISTA through resources
provided by the Imaging & Optics Facility and the Laboratory Support Facility. Bulk
RNA-seq was performed by the Next Generation Sequencing Facility at Vienna BioCenter
Core Facilities, member of the Vienna BioCenter. This work was supported by a European
Research Council Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by
the Austrian Science Fund (PE1028W1232 and PR1028FG1803) to G.N. Open access funding
provided by Institute of Science and Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Lena A
full_name: Schwarz, Lena A
id: 29A8453C-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Christoph
full_name: Dotter, Christoph
id: 4C66542E-F248-11E8-B48F-1D18A9856A87
last_name: Dotter
orcid: 0000-0002-9033-9096
- first_name: Sergey
full_name: Isaev, Sergey
last_name: Isaev
- first_name: Michela
full_name: Lisi, Michela
id: 39383c1b-d3eb-11ef-8d6c-c8cdf4e10c8c
last_name: Lisi
- first_name: Daniel
full_name: Malzl, Daniel
last_name: Malzl
- first_name: Christoph
full_name: Büschl, Christoph
id: 2a8c054c-0913-11ee-9159-f8ef515809ed
last_name: Büschl
- first_name: Sabrina
full_name: Ladstätter, Sabrina
last_name: Ladstätter
- first_name: Bárbara
full_name: Oliveira, Bárbara
id: 3B03AA1A-F248-11E8-B48F-1D18A9856A87
last_name: Oliveira
- first_name: Matteo
full_name: Barel, Matteo
id: 8959927b-2236-11ed-bd6e-ea83d94ade0e
last_name: Barel
- first_name: Bernadette
full_name: Basilico, Bernadette
id: 36035796-5ACA-11E9-A75E-7AF2E5697425
last_name: Basilico
orcid: 0000-0003-1843-3173
- first_name: Chaitanya
full_name: Chintaluri, Chaitanya
id: BA06AFEE-A4BA-11EA-AE5C-14673DDC885E
last_name: Chintaluri
orcid: 0000-0003-4252-1608
- first_name: Sarah
full_name: Gorkiewicz, Sarah
id: f141a35d-15a9-11ec-9fb2-fef6becc7b6f
last_name: Gorkiewicz
- first_name: Mohammad
full_name: Goudarzi, Mohammad
id: 3384113A-F248-11E8-B48F-1D18A9856A87
last_name: Goudarzi
- first_name: Tereza
full_name: Belinova, Tereza
id: 0bf89b6a-d28b-11eb-8bd6-f43768e4d368
last_name: Belinova
- first_name: Stephan
full_name: Reichl, Stephan
last_name: Reichl
- first_name: Gintarė
full_name: Sendžikaitė, Gintarė
id: dd6d52f2-c50d-11eb-9548-bcf0ff82b344
last_name: Sendžikaitė
- first_name: Satish
full_name: Arcot Jayaram, Satish
id: b0bbee33-09f7-11eb-909c-8b358058d28a
last_name: Arcot Jayaram
orcid: 0000-0002-2479-2669
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Tim P
full_name: Vogels, Tim P
id: CB6FF8D2-008F-11EA-8E08-2637E6697425
last_name: Vogels
orcid: 0000-0003-3295-6181
- first_name: Jörg
full_name: Menche, Jörg
last_name: Menche
- first_name: Igor
full_name: Adameyko, Igor
last_name: Adameyko
- first_name: Peter Vasili
full_name: Kharchenko, Peter Vasili
id: 0095641e-7eb7-11f1-8665-aec51a2ab5e0
last_name: Kharchenko
- first_name: Christoph
full_name: Bock, Christoph
last_name: Bock
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
citation:
ama: Schwarz LA, Dotter C, Isaev S, et al. Cortical development dynamics across
autism spectrum disorder mouse models. Nature. 2026. doi:10.1038/s41586-026-10679-1
apa: Schwarz, L. A., Dotter, C., Isaev, S., Lisi, M., Malzl, D., Büschl, C., … Novarino,
G. (2026). Cortical development dynamics across autism spectrum disorder mouse
models. Nature. Springer Nature. https://doi.org/10.1038/s41586-026-10679-1
chicago: Schwarz, Lena A, Christoph Dotter, Sergey Isaev, Michela Lisi, Daniel Malzl,
Christoph Büschl, Sabrina Ladstätter, et al. “Cortical Development Dynamics across
Autism Spectrum Disorder Mouse Models.” Nature. Springer Nature, 2026.
https://doi.org/10.1038/s41586-026-10679-1.
ieee: L. A. Schwarz et al., “Cortical development dynamics across autism
spectrum disorder mouse models,” Nature. Springer Nature, 2026.
ista: Schwarz LA, Dotter C, Isaev S, Lisi M, Malzl D, Büschl C, Ladstätter S, Oliveira
B, Barel M, Basilico B, Chintaluri C, Gorkiewicz S, Goudarzi M, Belinova T, Reichl
S, Sendžikaitė G, Arcot Jayaram S, Koppensteiner P, Sommer CM, Vogels TP, Menche
J, Adameyko I, Kharchenko PV, Bock C, Novarino G. 2026. Cortical development dynamics
across autism spectrum disorder mouse models. Nature.
mla: Schwarz, Lena A., et al. “Cortical Development Dynamics across Autism Spectrum
Disorder Mouse Models.” Nature, Springer Nature, 2026, doi:10.1038/s41586-026-10679-1.
short: L.A. Schwarz, C. Dotter, S. Isaev, M. Lisi, D. Malzl, C. Büschl, S. Ladstätter,
B. Oliveira, M. Barel, B. Basilico, C. Chintaluri, S. Gorkiewicz, M. Goudarzi,
T. Belinova, S. Reichl, G. Sendžikaitė, S. Arcot Jayaram, P. Koppensteiner, C.M.
Sommer, T.P. Vogels, J. Menche, I. Adameyko, P.V. Kharchenko, C. Bock, G. Novarino,
Nature (2026).
corr_author: '1'
dataavailabilitystatement: Single-nucleus multiomics data are available from the Gene
Expression Omnibus (GSE328363). The mm10 reference genome was used for the alignment
(refdata-cellranger-arc-mm10-2020-A-2.0.0, obtained from https://cf.10xgenomics.com/supp/cell-arc/refdata-cellranger-arc-mm10-2020-A-2.0.0.tar.gz).
Single-cell data can be accessed and visualized through a CELLxGENE database (https://adameykolab.hifo.meduniwien.ac.at/cellxgene_public/filecrawl/.2026_Nature_Schwarz).
Source data are provided with this paper. Scripts and analyses that support the
main findings of this study are accessible in a GitHub repository (https://git.ista.ac.at/research-sofware/mouseome).
date_created: 2026-07-13T09:47:21Z
date_published: 2026-06-17T00:00:00Z
date_updated: 2026-08-04T09:29:55Z
day: '17'
ddc:
- '570'
department:
- _id: AnKi
- _id: GaNo
- _id: TiVo
- _id: ScienComp
- _id: GradSch
- _id: Bio
- _id: PreCl
doi: 10.1038/s41586-026-10679-1
external_id:
pmid:
- '42310454'
fulldoi: https://doi.org/10.1038/s41586-026-10679-1
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-026-10679-1
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6
grant_number: '101044865'
name: Toward an understanding of the brain interstitial system and the extracellular
proteome in health and autism spectrum disorders
- _id: 9B91375C-BA93-11EA-9121-9846C619BF3A
grant_number: '707964'
name: Critical windows and reversibility of ASD associated with mutations in chromatin
remodelers
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232
name: Molecular Drug Targets
- _id: ebb38b5d-77a9-11ec-83b8-a42e08120a88
grant_number: FG1803 49015
name: Neurobiology of anxiety in autism spectrum disorders
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/patterns-in-genetic-chaos/
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Cortical development dynamics across autism spectrum disorder mouse models
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '22925'
abstract:
- lang: eng
text: Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed
detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged
DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal
without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence
in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated
image analysis pipeline that enables per-cell quantification of probe expression
and co-localization. This method provides a robust and quantitative approach for
visualizing gene expression patterns in situ.
acknowledgement: "This work was supported by a Horizon Europe ERC Starting Grant\r\nNumber
101041551 (L.B.S., D.V., S.P.), Special Research Program\r\n(SFB) of the Austrian
Science Fund (FWF) F7814-B (L.B.S., S.P.),\r\nAustrian Science Fund (FWF) 10.55776/COE16
(L.B.S.), FTI\r\nStrategy Lower Austria Dissertation Grant Number FT121-D-046\r\n(D.V.),
and Austrian Academy of Sciences DOC Fellowship 27229\r\n(S.P.). We also thank the
Imaging & Optics and Scientific Computing Facilities at ISTA for their support in
developing the quantification pipeline."
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
orcid: 0000-0002-5494-0941
- first_name: Stavros
full_name: Papadopoulos, Stavros
id: 40606b92-f128-11eb-9611-bf66a98cfa5c
last_name: Papadopoulos
- first_name: Marco
full_name: Dalla Vecchia, Marco
id: 02a7a869-ff06-11ed-a87f-86649d6077e5
last_name: Dalla Vecchia
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: 'Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. Fluorescent in situ
mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus
cryosections. In: Beck CW, ed. Xenopus. Vol 3049. MIMB. Springer; 2026:245-259.
doi:10.1007/978-1-0716-5360-9_11'
apa: Vijatovic, D., Papadopoulos, S., Dalla Vecchia, M., & Sweeney, L. B. (2026).
Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction
(HCR) in Xenopus cryosections. In C. W. Beck (Ed.), Xenopus (Vol. 3049,
pp. 245–259). Springer. https://doi.org/10.1007/978-1-0716-5360-9_11
chicago: Vijatovic, David, Stavros Papadopoulos, Marco Dalla Vecchia, and Lora B.
Sweeney. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain
Reaction (HCR) in Xenopus Cryosections.” In Xenopus, edited by Caroline
W. Beck, 3049:245–59. MIMB. Springer, 2026. https://doi.org/10.1007/978-1-0716-5360-9_11.
ieee: D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, and L. B. Sweeney, “Fluorescent
in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in
Xenopus cryosections,” in Xenopus, vol. 3049, C. W. Beck, Ed. Springer,
2026, pp. 245–259.
ista: 'Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. 2026.Fluorescent
in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in
Xenopus cryosections. In: Xenopus. Methods in Molecular Biology, vol. 3049, 245–259.'
mla: Vijatovic, David, et al. “Fluorescent in Situ MRNA Hybridization (FISH) Using
Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” Xenopus, edited
by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 245–59, doi:10.1007/978-1-0716-5360-9_11.
short: D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, L.B. Sweeney, in:, C.W.
Beck (Ed.), Xenopus, Springer, 2026, pp. 245–259.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-09-13T22:01:55Z
date_published: 2026-09-02T00:00:00Z
date_updated: 2026-09-17T07:31:16Z
day: '02'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: IAS
doi: 10.1007/978-1-0716-5360-9_11
editor:
- first_name: Caroline W.
full_name: Beck, Caroline W.
last_name: Beck
external_id:
pmid:
- '42681228'
file:
- access_level: open_access
checksum: e7a075a9ee1b91d5824f8f2b9b1f3c9b
content_type: application/pdf
creator: dernst
date_created: 2026-09-17T07:28:53Z
date_updated: 2026-09-17T07:28:53Z
file_id: '22938'
file_name: 2026_MIMB_Vijatovic.pdf
file_size: 1004024
relation: main_file
success: 1
file_date_updated: 2026-09-17T07:28:53Z
fulldoi: https://doi.org/10.1007/978-1-0716-5360-9_11
has_accepted_license: '1'
intvolume: ' 3049'
keyword:
- mRNA FISH
- Hybridization chain reaction
- In situ hybridization
- Fluorescence microscopy
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 245-259
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: cf428362-b037-11f1-b015-8277a8a2f63d
grant_number: COE16
name: Neuronal circuits in health and disease (Sweeney)
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: 907b765e-16d5-11f0-9cad-fef108a945b1
grant_number: '27229'
name: 'A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb
transition of Xenopus metamorphosis'
publication: Xenopus
publication_identifier:
eissn:
- 1940-6029
publication_status: published
publisher: Springer
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
series_title: MIMB
status: public
supplementarymaterial: no
title: Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction
(HCR) in Xenopus cryosections
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3049
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '23148'
abstract:
- lang: eng
text: Presenilin-1 (PS1) is an endoplasmic reticulum protein, most known for its
role in pathogenesis of familial Alzheimer’s Disease (AD). PS1 has been attributed
roles in intracellular calcium homeostasis in the brain, as well as in the pancreatic
beta cells, where it has been shown to be fundamental for the initial phase of
glucose-induced insulin secretion. To gain mechanistic insight into beta cell
autonomous function of PS1, we have examined various beta cell models and found
that PS1 controls glycolytic flux of pancreatic beta cells by regulating sub-mitochondrial
Ca2+ homeostasis. Transient knock down of PS1 yielded a glycolytic bottleneck
at the step catalyzed by glyceraldehyde-3-phosphate dehydrogenase, resulting in
defective glucose responsiveness. Moreover, we show that PS1 is needed for the
preservation of beta cell identity, likely due to its role in glycolysis. Finally,
we show that mouse islets from an in vivo model of AD mimic the defective glucose
responsiveness seen in beta cells with transient PS1 knock down, while the identity
loss and whole-body glucose homeostasis are not severely impacted, suggesting
an adaptive response. Thus, the current work highlights beta cell autonomous role
of PS1 and adds a layer of complexity in its impact on whole body metabolism.
acknowledgement: We appreciate the technical assistance from Anna Schreilechner. We
are grateful for support by all members of the animal facility team at IMB, University
of Graz, as well as K. Zierler for assistance with animal licenses. F.M, T.E.,
T.M., T.P., and W.F.G. are grateful to the Austrian Science Fund (FWF) for the excellence
cluster 10.55776/COE14 (MetAGE), which was additionally supported by Land Styria,
Stadt Graz, University of Graz and Medical University of Graz. This research was
further supported by the FWF via the DKplus program 10.55776/W1226 (DK-MCD) to F.M.,
T.M., R.B.G., and W.F.G., 10.55776/P28529, 10.55776/I3716 (to R.M.), 10.55776/P28854,
10.55776/I3792, 10.55776/DOC130 (to T.M.), and 10.55776/I4319 (to. M.S.R.); the
Austrian Research Promotion Agency (FFG) grants 864690 and 870454; the Integrative
Metabolism Research Center Graz; the Austrian Infrastructure Program 2016/2017 (to
T.M.), and 10.55776/COE7, 10.55776/COE17, 10.55776/FG12 and 10.55776/F73 (to R.B.G.);
the Styrian Government; the City of Graz; and BioTechMed-Graz (flagship project)
(to T.M.); the MEFO Graz (to W.F.G.). F.E.O., J.G. and Z.K. were doctoral fellows
in the doctoral program Metabolic and Cardiovascular Disease (MCD) (FWF, 10.55776/W
1226) at the Medical University of Graz. S.v.A. and A.S. are thesis students in
the excellence cluster 10.55776/COE14 (MetAGE, W.F.G.). M.H. was a fellow of the
Molecular Medicine (MolMed) doctoral program at the Medical University of Graz supported
by MEFO Graz. The SIM equipment is part of the Nikon Center of Excellence, Graz,
and is supported by the Austrian infrastructure program 2013/ 2014, Nikon Austria
Inc., and BioTechMed (to W.F.G.). Our thanks go to Shimadzu Austria for supporting
this research through instrument access given in the Metabolomics and Bioprocess
Analytics laboratory (TU Wien) (R.B.G.). For open access purposes, the authors have
applied a CC BY public copyright license to any author-accepted manuscript version
arising from this submission.” We thank the Medical University of Graz for financial
support.
article_number: '1315'
article_processing_charge: Yes
article_type: original
author:
- first_name: Zhanat
full_name: Koshenov, Zhanat
last_name: Koshenov
- first_name: Sandra
full_name: Postic, Sandra
last_name: Postic
- first_name: Gabriela
full_name: Schoiswohl, Gabriela
last_name: Schoiswohl
- first_name: Savina
full_name: Van Amsterdam, Savina
last_name: Van Amsterdam
- first_name: Victoria
full_name: Hois-Zelinka, Victoria
last_name: Hois-Zelinka
- first_name: Furkan E.
full_name: Oflaz, Furkan E.
last_name: Oflaz
- first_name: Rene
full_name: Rost, Rene
last_name: Rost
- first_name: Oleksandra
full_name: Tiapko, Oleksandra
last_name: Tiapko
- first_name: Benjamin
full_name: Gottschalk, Benjamin
last_name: Gottschalk
- first_name: Martin
full_name: Hirtl, Martin
last_name: Hirtl
- first_name: Olaf G.
full_name: Bachkoenig, Olaf G.
last_name: Bachkoenig
- first_name: Aigerim
full_name: Koshenova, Aigerim
last_name: Koshenova
- first_name: Yusuf C.
full_name: Erdogan, Yusuf C.
last_name: Erdogan
- first_name: Adlet
full_name: Sagintayev, Adlet
last_name: Sagintayev
- first_name: Anita
full_name: Krnjic, Anita
last_name: Krnjic
- first_name: Johannes U.
full_name: Pfabe, Johannes U.
last_name: Pfabe
- first_name: Srdjan
full_name: Sarikas, Srdjan
id: 35F0286E-F248-11E8-B48F-1D18A9856A87
last_name: Sarikas
- first_name: Bernhard
full_name: Hochreiter, Bernhard
id: e6cab3de-17f6-11ed-9210-c1e42e045e9d
last_name: Hochreiter
- first_name: Juergen
full_name: Gindlhuber, Juergen
last_name: Gindlhuber
- first_name: Matthias
full_name: Schittmayer, Matthias
last_name: Schittmayer
- first_name: Jelena
full_name: Tadic, Jelena
last_name: Tadic
- first_name: Barbara
full_name: Ehall, Barbara
last_name: Ehall
- first_name: Frank
full_name: Madeo, Frank
last_name: Madeo
- first_name: Tobias
full_name: Madl, Tobias
last_name: Madl
- first_name: Roland
full_name: Malli, Roland
last_name: Malli
- first_name: Ruth
full_name: Birner-Gruenberger, Ruth
last_name: Birner-Gruenberger
- first_name: Thomas
full_name: Pieber, Thomas
last_name: Pieber
- first_name: Tobias
full_name: Eisenberg, Tobias
last_name: Eisenberg
- first_name: Marjan
full_name: Slak Rupnik, Marjan
last_name: Slak Rupnik
- first_name: Wolfgang F.
full_name: Graier, Wolfgang F.
last_name: Graier
citation:
ama: Koshenov Z, Postic S, Schoiswohl G, et al. Presenilin-1 controls glycolysis
and identity of pancreatic beta cells. Communications Biology. 2026;9.
doi:10.1038/s42003-026-10581-6
apa: Koshenov, Z., Postic, S., Schoiswohl, G., Van Amsterdam, S., Hois-Zelinka,
V., Oflaz, F. E., … Graier, W. F. (2026). Presenilin-1 controls glycolysis and
identity of pancreatic beta cells. Communications Biology. Springer Nature.
https://doi.org/10.1038/s42003-026-10581-6
chicago: Koshenov, Zhanat, Sandra Postic, Gabriela Schoiswohl, Savina Van Amsterdam,
Victoria Hois-Zelinka, Furkan E. Oflaz, Rene Rost, et al. “Presenilin-1 Controls
Glycolysis and Identity of Pancreatic Beta Cells.” Communications Biology.
Springer Nature, 2026. https://doi.org/10.1038/s42003-026-10581-6.
ieee: Z. Koshenov et al., “Presenilin-1 controls glycolysis and identity
of pancreatic beta cells,” Communications Biology, vol. 9. Springer Nature,
2026.
ista: Koshenov Z, Postic S, Schoiswohl G, Van Amsterdam S, Hois-Zelinka V, Oflaz
FE, Rost R, Tiapko O, Gottschalk B, Hirtl M, Bachkoenig OG, Koshenova A, Erdogan
YC, Sagintayev A, Krnjic A, Pfabe JU, Sarikas S, Hochreiter B, Gindlhuber J, Schittmayer
M, Tadic J, Ehall B, Madeo F, Madl T, Malli R, Birner-Gruenberger R, Pieber T,
Eisenberg T, Slak Rupnik M, Graier WF. 2026. Presenilin-1 controls glycolysis
and identity of pancreatic beta cells. Communications Biology. 9, 1315.
mla: Koshenov, Zhanat, et al. “Presenilin-1 Controls Glycolysis and Identity of
Pancreatic Beta Cells.” Communications Biology, vol. 9, 1315, Springer
Nature, 2026, doi:10.1038/s42003-026-10581-6.
short: Z. Koshenov, S. Postic, G. Schoiswohl, S. Van Amsterdam, V. Hois-Zelinka,
F.E. Oflaz, R. Rost, O. Tiapko, B. Gottschalk, M. Hirtl, O.G. Bachkoenig, A. Koshenova,
Y.C. Erdogan, A. Sagintayev, A. Krnjic, J.U. Pfabe, S. Sarikas, B. Hochreiter,
J. Gindlhuber, M. Schittmayer, J. Tadic, B. Ehall, F. Madeo, T. Madl, R. Malli,
R. Birner-Gruenberger, T. Pieber, T. Eisenberg, M. Slak Rupnik, W.F. Graier, Communications
Biology 9 (2026).
das_tickbox: '1'
dataavailabilitystatement: The data supporting the findings of this research can be
obtained from the authors upon a reasonable request; please refer to the author’s
contributions for details regarding specific datasets. Numerical source data underlying
graphs in this article can be found in Supplementary data file. Supplementary Figs.
and figure legends are included in the Supplementary file. The code used in this
study is available from the authors on reasonable request.
date_created: 2026-10-18T22:01:50Z
date_published: 2026-10-08T00:00:00Z
date_updated: 2026-10-19T06:23:26Z
day: '08'
ddc:
- '570'
department:
- _id: FISS
doi: 10.1038/s42003-026-10581-6
external_id:
pmid:
- '42387039'
file:
- access_level: open_access
checksum: b90e60a625fcac8ef5d9ff968792d675
content_type: application/pdf
creator: dernst
date_created: 2026-10-19T06:19:39Z
date_updated: 2026-10-19T06:19:39Z
file_id: '23161'
file_name: 2026_CommunicationsBiology_Koshenov.pdf
file_size: 1819866
relation: main_file
success: 1
file_date_updated: 2026-10-19T06:19:39Z
fulldoi: https://doi.org/10.1038/s42003-026-10581-6
has_accepted_license: '1'
intvolume: ' 9'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Communications Biology
publication_identifier:
eissn:
- 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Presenilin-1 controls glycolysis and identity of pancreatic beta cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '23171'
abstract:
- lang: eng
text: Crawling motility is a hallmark of eukaryotic cells and requires a dynamic
actin cytoskeleton, regulated adhesion and spatially organized signalling pathways1,2.
Asgard archaea, which are considered the closest known prokaryotic relatives of
eukaryotes, potentially encode these functions within their large set of ‘eukaryotic
signature proteins’3,4,5,6. The few cultivated members show a complex cell morphology,
consisting of a central cell body from which several protrusions extend, filled
with an actin-based cytoskeleton7,8. Here, live-cell microscopy of two organisms
of the Lokiarchaea and Hodarchaea lineages7,9 showed that they dynamically and
greatly change their cell shape on a minute time scale and grow and retract their
extensive protrusions with a speed of 1.5–4.8 µm min−1, respectively. After adhering
to a glass surface, cells use their protrusions to undergo active crawling motion.
In the presence of selected actin inhibitors, however, the observed dynamics were
arrested, suggesting a central role of actin in these processes. The observed
cellular plasticity and motility are unique features among prokaryotes and might
have been crucial for the emergence of the first eukaryotic cells that are thought
to have formed through the association of a member of the Asgard archaea and an
α-proteobacterium, the ancestor of mitochondria.
acknowledged_ssus:
- _id: Bio
acknowledgement: This work would not have been possible without the technical support
in cultivation from K. Hager, F. Baraket, A. Malits and S. Harrer. We thank the
Imaging and Optics Facility (IOF) at ISTA for their help in setting up the anaerobic
environment during imaging and the Cell Imaging & Ultrastructure Research (CIUS)
facility at the University of Vienna for their support. Proteomics analyses were
performed by the Mass Spectrometry Facility at Max Perutz Labs using the VBCF instrument
pool. We also thank P. Klahn (University of Gothenburg, Sweden), M. Stadler and
members of his department (HZI Braunschweig) as well as R. Cox (LUH Hannover) for
providing different cytochalasans and P. Bieling (King’s College London) for discussion.
Most of this work was funded by the Austrian Science Fund (FWF) with grant DOI 10.55776/Z437
(available at https://doi.org/10.55776/Z437) and further supported by an emerging
field grant with grant DOI 10.55776/EFP25 (available at https://doi.org/10.55776/EFP25)
to C.S. For open access purposes, the author has applied a CC BY public copyright
license to any author accepted manuscript version arising from this submission.
This work was further supported by a European Research Council grant ERC-SyG (101071793)
to M.S., by the German Research Council through the CytoLabs consortium (Research
Unit 5170 to K.R. and T.E.B.S.), by the Japan Society for the Promotion of Science
Grant-in-Aid for Scientific Research 22H04985 to H.I. and M.N. Furthermore, R.H.
was supported by CZI grant DAF2020-225401 and a grant (https://doi.org/10.37921/120055ratwvi)
from the Chan Zuckerberg Initiative DAF. Open access funding provided by University
of Vienna.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Philipp
full_name: Radler, Philipp
id: 40136C2A-F248-11E8-B48F-1D18A9856A87
last_name: Radler
orcid: '0000-0001-9198-2182 '
- first_name: Tobias
full_name: Viehboeck, Tobias
last_name: Viehboeck
- first_name: Zhen-Hao
full_name: Luo, Zhen-Hao
last_name: Luo
- first_name: Nevena
full_name: Maslać, Nevena
last_name: Maslać
- first_name: Katharina
full_name: Schmidt, Katharina
last_name: Schmidt
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Masaru K.
full_name: Nobu, Masaru K.
last_name: Nobu
- first_name: Silvia
full_name: Bulgheresi, Silvia
last_name: Bulgheresi
- first_name: Theresia E. B.
full_name: Stradal, Theresia E. B.
last_name: Stradal
- first_name: Klemens
full_name: Rottner, Klemens
last_name: Rottner
- first_name: Hiroyuki
full_name: Imachi, Hiroyuki
last_name: Imachi
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Christa
full_name: Schleper, Christa
last_name: Schleper
citation:
ama: Radler P, Viehboeck T, Luo Z-H, et al. Dynamic protrusions mediate crawling
motility in Asgard archaea. Nature. 2026. doi:10.1038/s41586-026-11063-9
apa: Radler, P., Viehboeck, T., Luo, Z.-H., Maslać, N., Schmidt, K., Hauschild,
R., … Schleper, C. (2026). Dynamic protrusions mediate crawling motility in Asgard
archaea. Nature. Springer Nature. https://doi.org/10.1038/s41586-026-11063-9
chicago: Radler, Philipp, Tobias Viehboeck, Zhen-Hao Luo, Nevena Maslać, Katharina
Schmidt, Robert Hauschild, Masaru K. Nobu, et al. “Dynamic Protrusions Mediate
Crawling Motility in Asgard Archaea.” Nature. Springer Nature, 2026. https://doi.org/10.1038/s41586-026-11063-9.
ieee: P. Radler et al., “Dynamic protrusions mediate crawling motility in
Asgard archaea,” Nature. Springer Nature, 2026.
ista: Radler P, Viehboeck T, Luo Z-H, Maslać N, Schmidt K, Hauschild R, Nobu MK,
Bulgheresi S, Stradal TEB, Rottner K, Imachi H, Sixt MK, Schleper C. 2026. Dynamic
protrusions mediate crawling motility in Asgard archaea. Nature.
mla: Radler, Philipp, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard
Archaea.” Nature, Springer Nature, 2026, doi:10.1038/s41586-026-11063-9.
short: P. Radler, T. Viehboeck, Z.-H. Luo, N. Maslać, K. Schmidt, R. Hauschild,
M.K. Nobu, S. Bulgheresi, T.E.B. Stradal, K. Rottner, H. Imachi, M.K. Sixt, C.
Schleper, Nature (2026).
das_tickbox: '1'
dataavailabilitystatement: The plots in this manuscript were created using Jupyter
Notebook; the source data for all figures have been provided in data source tables.
Microscopy raw data for supplementary videos, the Jupyter notebooks to generate
the plots and the raw files for the structural phylogenetic analysis are available
at https://doi.org/10.6084/m9.figshare.30676367. The remaining raw microscopy data
generated for this manuscript are available from https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD3928.
The mass spectrometry proteomics data from the Co-IP experiments have been deposited
to the ProteomeXchange Consortium via the PRIDE partner repository79 and are available
at https://www.ebi.ac.uk/pride/archive/projects/PXD068820. The mass spectrometry
proteomics data of the proteome analysis is available at https://www.ebi.ac.uk/pride/archive/projects/PXD074767.
Source data are provided with this paper.
date_created: 2026-10-19T09:26:53Z
date_published: 2026-09-30T00:00:00Z
date_updated: 2026-10-19T09:40:50Z
day: '30'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
doi: 10.1038/s41586-026-11063-9
external_id:
pmid:
- '42816618'
fulldoi: https://doi.org/10.1038/s41586-026-11063-9
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-026-11063-9
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd
grant_number: '101071793'
name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal
pushing forces'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Dynamic protrusions mediate crawling motility in Asgard archaea
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20859'
abstract:
- lang: eng
text: Effective immune responses rely on the efficient migration of leukocytes.
Yet, how temperature regulates migration dynamics at the single-cell level has
remained poorly understood. Using zebrafish embryos and mouse tissue explants,
we found that temperature positively regulates leukocyte migration speed, exploration,
and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures
revealed that this thermokinetic control of cell migration is conserved across
immune cell types, independently of the 3D tissue environment. By applying precise
(sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response
that depends on myosin II activity. Small physiological increases in temperature
(1°C –2°C), as present during fever-like conditions, profoundly increased immune
responses by accelerating arrival times at lymphatic vessels and tissue wounds.
These findings identify myosin-II-dependent actomyosin contractility as a critical
mechanical structure regulating single-cell thermo-adaptability, with physiological
implications for tuning the speed of immune responses in vivo.
acknowledged_ssus:
- _id: NanoFab
acknowledgement: 'The authors would like to acknowledge the Super Resolution Light
Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments,
Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of
molds for generating confinement coverslip, Merche Rivas for cell culture of immune
cells and further support from the CRG Core Facilities for Genomics and Advanced
Light Microscopy. We would like to thank Michael Sixt for discussions on this work
and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript.
This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges
the funding from the European Union’s Horizon 2020 research and innovation programme
under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone
– PhD Programme funded by the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges
support through the Spanish Ministry of Economy and Competitiveness via MINECO’s
Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s
HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and
European Union''s Horizon Europe program under the grant agreement no. 101072123.
E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science
and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche
Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence
Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn)
as part of the Excellence Strategy of the federal and state governments.'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Iván
full_name: Company-Garrido, Iván
last_name: Company-Garrido
- first_name: Alberto
full_name: Zurita Carpio, Alberto
last_name: Zurita Carpio
- first_name: Mariona
full_name: Colomer-Rosell, Mariona
last_name: Colomer-Rosell
- first_name: Bernard
full_name: Ciraulo, Bernard
last_name: Ciraulo
- first_name: Ronja
full_name: Molkenbur, Ronja
last_name: Molkenbur
- first_name: Peter
full_name: Lanzerstorfer, Peter
last_name: Lanzerstorfer
- first_name: Fabio
full_name: Pezzano, Fabio
last_name: Pezzano
- first_name: Costanza
full_name: Agazzi, Costanza
last_name: Agazzi
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Saumey
full_name: Jain, Saumey
last_name: Jain
- first_name: Jeroen M.
full_name: Jacques, Jeroen M.
last_name: Jacques
- first_name: Valeria
full_name: Venturini, Valeria
last_name: Venturini
- first_name: Christian
full_name: Knapp, Christian
last_name: Knapp
- first_name: Yufei
full_name: Xie, Yufei
last_name: Xie
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Julian
full_name: Weghuber, Julian
last_name: Weghuber
- first_name: Marcel
full_name: Schaaf, Marcel
last_name: Schaaf
- first_name: Romain
full_name: Quidant, Romain
last_name: Quidant
- first_name: Eva
full_name: Kiermaier, Eva
id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
last_name: Kiermaier
orcid: 0000-0001-6165-5738
- first_name: Jaime
full_name: Ortega Arroyo, Jaime
last_name: Ortega Arroyo
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
citation:
ama: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates
cellular thermo-adaptability and the efficiency of immune responses. Developmental
Cell. 2026;61(2):356-371.e12. doi:10.1016/j.devcel.2025.10.006
apa: Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur,
R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability
and the efficiency of immune responses. Developmental Cell. Cell Press.
https://doi.org/10.1016/j.devcel.2025.10.006
chicago: Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard
Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II
Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.”
Developmental Cell. Cell Press, 2026. https://doi.org/10.1016/j.devcel.2025.10.006.
ieee: I. Company-Garrido et al., “Myosin II regulates cellular thermo-adaptability
and the efficiency of immune responses,” Developmental Cell, vol. 61, no.
2. Cell Press, p. 356–371.e12, 2026.
ista: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur
R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini
V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega
Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability
and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.
mla: Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability
and the Efficiency of Immune Responses.” Developmental Cell, vol. 61, no.
2, Cell Press, 2026, p. 356–371.e12, doi:10.1016/j.devcel.2025.10.006.
short: I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur,
P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques,
V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant,
E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61
(2026) 356–371.e12.
das_tickbox: '1'
dataavailabilitystatement: "This study did not generate new unique reagents. Data
are available upon request.\r\n•The custom-made codes used in this study are available
at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n•
Any additional information required to reanalyze the data reported in this paper
is available from the lead contact upon request."
date_created: 2025-12-28T23:01:27Z
date_published: 2026-02-11T00:00:00Z
date_updated: 2026-10-20T09:01:14Z
day: '11'
ddc:
- '570'
department:
- _id: Bio
- _id: NanoFab
doi: 10.1016/j.devcel.2025.10.006
external_id:
pmid:
- '41192429'
file:
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checksum: 52fd52d2d19a4514f8fcc1b40f420ca2
content_type: application/pdf
creator: dernst
date_created: 2026-07-23T06:26:25Z
date_updated: 2026-07-23T06:26:25Z
file_id: '22388'
file_name: 2026_DevelopmentalCell_CompanyGarrido.pdf
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file_date_updated: 2026-07-23T06:26:25Z
fulldoi: https://doi.org/10.1016/j.devcel.2025.10.006
has_accepted_license: '1'
intvolume: ' 61'
issue: '2'
keyword:
- thermobiology
- cell migration
- thermo-adaptability of immune cells
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 356-371.e12
pmid: 1
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Cell Press
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune
responses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 61
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21746'
abstract:
- lang: eng
text: As vertebrates transitioned from water to land, locomotion shifted from undulatory
swimming to limb-based movement. How spinal circuits and their cell types evolved
to support this transition remains unclear. We leverage frog metamorphosis, which
recapitulates this transition within a single organism, to define how spinal circuits
generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture
is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons.
As limbs develop and their movement complexifies, spinal circuits expand in neuron
number and subtype diversity. This expansion is most pronounced for V1 inhibitory
neurons, which increase ∼70-fold and diversify into transcriptionally distinct
subtypes. Disrupting transcription factors defining emerging motor and V1 populations
reveals molecular segregation between swim and limb circuits, highlighting the
role of subtype diversity in motor coordination. A multifold increase in inhibitory
neuron diversity thus underlies the tail-to-limb locomotor transition, providing
a framework for spinal circuit adaptation during vertebrate evolution.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de
Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript.
We are also grateful to Tom Jessell and Chris Kintner for their scientific insight
and mentorship during the conception of this project. It would also have not been
possible without the technical support of the Aquatics and Imaging and Optics Facility
support teams (ISTA). We thank Martin Estermann for preparing the initial draft
of the graphical abstract and Niki Barolini for the final version. In addition,
we thank our funding sources for providing the resources to do these experiments:
GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon
Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special
Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.),
Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858
(to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH
grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC)
(to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472
(to Z.H.), and Project A.L.S. (to S.B.-M.).'
article_number: '117227'
article_processing_charge: Yes
article_type: original
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
orcid: 0000-0002-5494-0941
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Y
full_name: Ignatyev, Y
last_name: Ignatyev
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matthijs Geert
full_name: Smits, Matthijs Geert
id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
last_name: Smits
- first_name: Marco
full_name: Dalla Vecchia, Marco
id: 02a7a869-ff06-11ed-a87f-86649d6077e5
last_name: Dalla Vecchia
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227
apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M.,
Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2026.117227
chicago: Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington,
Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold
Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell
Reports. Cell Press, 2026. https://doi.org/10.1016/j.celrep.2026.117227.
ieee: D. Vijatovic et al., “Multifold increase in spinal inhibitory cell
types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Cell
Press, 2026.
ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits
MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto
MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 45(4), 117227.
mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types
with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227,
Cell Press, 2026, doi:10.1016/j.celrep.2026.117227.
short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild,
M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton,
M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).
corr_author: '1'
date_created: 2026-04-19T22:07:43Z
date_published: 2026-04-28T00:00:00Z
date_updated: 2026-10-20T09:04:23Z
day: '28'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1016/j.celrep.2026.117227
external_id:
pmid:
- '41964955 '
file:
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checksum: 0d26cdb5b8d8dec3a911d8261a65cdef
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creator: dernst
date_created: 2026-05-04T12:20:10Z
date_updated: 2026-05-04T12:20:10Z
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file_size: 14925958
relation: main_file
success: 1
file_date_updated: 2026-05-04T12:20:10Z
fulldoi: https://doi.org/10.1016/j.celrep.2026.117227
has_accepted_license: '1'
intvolume: ' 45'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: cf428362-b037-11f1-b015-8277a8a2f63d
grant_number: COE16
name: Neuronal circuits in health and disease (Sweeney)
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '22667'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Multifold increase in spinal inhibitory cell types with emergence of limb movement
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
OA_type: closed access
_id: '20870'
abstract:
- lang: eng
text: RNA sequencing (RNA-seq) methodologies have evolved rapidly, offering powerful
tools to study gene expression, transcriptome dynamics, and molecular mechanisms
in various biological contexts. However, the complexity of these approaches poses
challenges in data interpretation, sensitivity, and applicability. This chapter
provides a comprehensive overview of RNA-seq methodologies, highlighting their
advantages, limitations, and applications, particularly in cardiovascular research.
Bulk RNA sequencing enables high-throughput gene expression profiling but lacks
the resolution to capture cellular heterogeneity and spatial context. Direct RNA
sequencing preserves native RNA modifications, offering insights into post-transcriptional
regulation, though it remains technically challenging. Single-cell RNA sequencing
(scRNA-seq) and spatial transcriptomics (ST) bridge these gaps by resolving transcriptomic
complexity at the cellular level and within tissue architecture, providing crucial
insights into disease mechanisms such as atherosclerosis. By summarizing the strengths
and limitations of these methodologies, this chapter aims to guide researchers
in selecting the most suitable transcriptomic approach for their studies, ultimately
advancing precision medicine and biomarker discovery in cardiovascular disease.
article_processing_charge: No
author:
- first_name: Victoria
full_name: Stopa, Victoria
last_name: Stopa
- first_name: Miron
full_name: Sopić, Miron
last_name: Sopić
- first_name: Guanliang
full_name: Li, Guanliang
last_name: Li
- first_name: Judith
full_name: Sluimer, Judith
last_name: Sluimer
- first_name: José
full_name: Basílio, José
last_name: Basílio
- first_name: Sander W.
full_name: van der Laan, Sander W.
last_name: van der Laan
- first_name: David P.
full_name: Kreil, David P.
last_name: Kreil
- first_name: Yvan
full_name: Devaux, Yvan
last_name: Devaux
- first_name: Bernhard
full_name: Hochreiter, Bernhard
id: e6cab3de-17f6-11ed-9210-c1e42e045e9d
last_name: Hochreiter
citation:
ama: 'Stopa V, Sopić M, Li G, et al. Essentials of transcriptomic methods: Navigating
through RNA sequencing and beyond. In: Devaux Y, Sopic M, eds. Transcriptomics
in Atherosclerosis. Elsevier; 2025:131-172. doi:10.1016/b978-0-443-33064-3.00016-5'
apa: 'Stopa, V., Sopić, M., Li, G., Sluimer, J., Basílio, J., van der Laan, S. W.,
… Hochreiter, B. (2025). Essentials of transcriptomic methods: Navigating through
RNA sequencing and beyond. In Y. Devaux & M. Sopic (Eds.), Transcriptomics
in Atherosclerosis (pp. 131–172). Elsevier. https://doi.org/10.1016/b978-0-443-33064-3.00016-5'
chicago: 'Stopa, Victoria, Miron Sopić, Guanliang Li, Judith Sluimer, José Basílio,
Sander W. van der Laan, David P. Kreil, Yvan Devaux, and Bernhard Hochreiter.
“Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.”
In Transcriptomics in Atherosclerosis, edited by Yvan Devaux and Miron
Sopic, 131–72. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-33064-3.00016-5.'
ieee: 'V. Stopa et al., “Essentials of transcriptomic methods: Navigating
through RNA sequencing and beyond,” in Transcriptomics in Atherosclerosis,
Y. Devaux and M. Sopic, Eds. Elsevier, 2025, pp. 131–172.'
ista: 'Stopa V, Sopić M, Li G, Sluimer J, Basílio J, van der Laan SW, Kreil DP,
Devaux Y, Hochreiter B. 2025.Essentials of transcriptomic methods: Navigating
through RNA sequencing and beyond. In: Transcriptomics in Atherosclerosis. , 131–172.'
mla: 'Stopa, Victoria, et al. “Essentials of Transcriptomic Methods: Navigating
through RNA Sequencing and Beyond.” Transcriptomics in Atherosclerosis,
edited by Yvan Devaux and Miron Sopic, Elsevier, 2025, pp. 131–72, doi:10.1016/b978-0-443-33064-3.00016-5.'
short: V. Stopa, M. Sopić, G. Li, J. Sluimer, J. Basílio, S.W. van der Laan, D.P.
Kreil, Y. Devaux, B. Hochreiter, in:, Y. Devaux, M. Sopic (Eds.), Transcriptomics
in Atherosclerosis, Elsevier, 2025, pp. 131–172.
date_created: 2025-12-29T12:16:22Z
date_published: 2025-10-24T00:00:00Z
date_updated: 2026-01-05T11:49:54Z
day: '24'
department:
- _id: Bio
doi: 10.1016/b978-0-443-33064-3.00016-5
editor:
- first_name: Yvan
full_name: Devaux, Yvan
last_name: Devaux
- first_name: Miron
full_name: Sopic, Miron
last_name: Sopic
fulldoi: https://doi.org/10.1016/b978-0-443-33064-3.00016-5
language:
- iso: eng
month: '10'
oa_version: None
page: 131-172
publication: Transcriptomics in Atherosclerosis
publication_identifier:
isbn:
- '9780443330643'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Essentials of transcriptomic methods: Navigating through RNA sequencing and
beyond'
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: repository
OA_type: gold
_id: '19294'
abstract:
- lang: eng
text: Active regulation of gene expression, orchestrated by complex interactions
of activators and repressors at promoters, controls the fate of organisms. In
contrast, basal expression at uninduced promoters is considered to be a dynamically
inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional
regulation. Here, we investigate the basal expression mode of the mar operon,
the main regulator of intrinsic multiple antibiotic resistance in Escherichia
coli, and link its dynamic properties to the non-canonical, yet highly conserved
start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements
across tens of generations reveal that basal expression consists of rare stochastic
gene expression pulses, which maximize variability in wildtype and, surprisingly,
transiently accelerate cellular elongation rates. Competition experiments show
that basal expression confers fitness advantages to wildtype across several transitions
between exponential and stationary growth by shortening lag times. The dynamically
rich basal expression of the mar operon has likely been evolutionarily maintained
for its role in growth homeostasis of Enterobacteria within the gut environment,
thereby allowing other ancillary gene regulatory roles to evolve, e.g. control
of costly-to-induce multi-drug efflux pumps. Understanding the complex selection
forces governing genetic systems involved in intrinsic multi-drug resistance is
crucial for effective public health measures.
article_processing_charge: No
author:
- first_name: Kirti
full_name: Jain, Kirti
id: 330F0278-F248-11E8-B48F-1D18A9856A87
last_name: Jain
orcid: 0000-0002-3809-0449
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Roderich
full_name: Römhild, Roderich
id: 68E56E44-62B0-11EA-B963-444F3DDC885E
last_name: Römhild
orcid: 0000-0001-9480-5261
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile
basal gene expression as a fitness determinant in bacteria.” 2025. doi:10.15479/AT:ISTA:19294
apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet,
C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant
in bacteria.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:19294
chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper
Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025.
https://doi.org/10.15479/AT:ISTA:19294.
ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet,
“Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’”
Institute of Science and Technology Austria, 2025.
ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data
for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:19294.
mla: Jain, Kirti, et al. Data for “Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.” Institute of Science and Technology Austria, 2025,
doi:10.15479/AT:ISTA:19294.
short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).
corr_author: '1'
date_created: 2025-03-04T13:27:21Z
date_published: 2025-03-04T00:00:00Z
date_updated: 2026-05-20T08:33:07Z
day: '04'
ddc:
- '570'
department:
- _id: CaGu
- _id: Bio
- _id: FyKo
- _id: GaTk
doi: 10.15479/AT:ISTA:19294
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oa: 1
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title: Data for "Pulsatile basal gene expression as a fitness determinant in bacteria"
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legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
APC_amount: 5949 EUR
OA_place: publisher
OA_type: hybrid
_id: '19626'
abstract:
- lang: eng
text: Active regulation of gene expression, orchestrated by complex interactions
of activators and repressors at promoters, controls the fate of organisms. In
contrast, basal expression at uninduced promoters is considered to be a dynamically
inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional
regulation. Here, we investigate the basal expression mode of the mar operon,
the main regulator of intrinsic multiple antibiotic resistance in Escherichia
coli, and link its dynamic properties to the noncanonical, yet highly conserved
start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements
across tens of generations reveal that basal expression consists of rare stochastic
gene expression pulses, which maximize variability in wildtype and, surprisingly,
transiently accelerate cellular elongation rates. Competition experiments show
that basal expression confers fitness advantages to wildtype across several transitions
between exponential and stationary growth by shortening lag times. The dynamically
rich basal expression of the mar operon has likely been evolutionarily maintained
for its role in growth homeostasis of Enterobacteria within the gut environment,
thereby allowing other ancillary gene regulatory roles to evolve, e.g., control
of costly-to-induce multidrug efflux pumps. Understanding the complex selection
forces governing genetic systems involved in intrinsic multidrug resistance is
crucial for effective public health measures.
acknowledged_ssus:
- _id: Bio
acknowledgement: K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions
on the manuscript, all other members from the Guet laboratory for valuable feedback,
R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria
for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic
Technology India for allowing time off to address the revisions. K.J. acknowledges
Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported
in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi),
O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant
ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.
article_number: e2413709122
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Kirti
full_name: Jain, Kirti
id: 330F0278-F248-11E8-B48F-1D18A9856A87
last_name: Jain
orcid: 0000-0002-3809-0449
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Roderich
full_name: Römhild, Roderich
id: 68E56E44-62B0-11EA-B963-444F3DDC885E
last_name: Römhild
orcid: 0000-0001-9480-5261
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile
basal gene expression as a fitness determinant in bacteria. Proceedings of
the National Academy of Sciences. 2025;122(15). doi:10.1073/pnas.2413709122
apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet,
C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria.
Proceedings of the National Academy of Sciences. National Academy of Sciences.
https://doi.org/10.1073/pnas.2413709122
chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper
Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant
in Bacteria.” Proceedings of the National Academy of Sciences. National
Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2413709122.
ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet,
“Pulsatile basal gene expression as a fitness determinant in bacteria,” Proceedings
of the National Academy of Sciences, vol. 122, no. 15. National Academy of
Sciences, 2025.
ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile
basal gene expression as a fitness determinant in bacteria. Proceedings of the
National Academy of Sciences. 122(15), e2413709122.
mla: Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant
in Bacteria.” Proceedings of the National Academy of Sciences, vol. 122,
no. 15, e2413709122, National Academy of Sciences, 2025, doi:10.1073/pnas.2413709122.
short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings
of the National Academy of Sciences 122 (2025).
corr_author: '1'
date_created: 2025-04-27T22:02:13Z
date_published: 2025-04-15T00:00:00Z
date_updated: 2026-05-20T08:33:08Z
day: '15'
ddc:
- '570'
department:
- _id: CaGu
- _id: Bio
- _id: FyKo
- _id: GaTk
doi: 10.1073/pnas.2413709122
external_id:
isi:
- '001471235200001'
pmid:
- '40193613'
file:
- access_level: open_access
checksum: 115a687f40009660eb4b38b4f6559d41
content_type: application/pdf
creator: dernst
date_created: 2025-06-24T07:27:43Z
date_updated: 2025-06-24T07:27:43Z
file_id: '19888'
file_name: 2025_PNAS_Jain.pdf
file_size: 2949523
relation: main_file
success: 1
file_date_updated: 2025-06-24T07:27:43Z
fulldoi: https://doi.org/10.1073/pnas.2413709122
has_accepted_license: '1'
intvolume: ' 122'
isi: 1
issue: '15'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
- _id: bd6f94d1-d553-11ed-ba76-ae9f07250f74
grant_number: E219
name: Non-canonical antibiotic interactions
- _id: 34e076d6-11ca-11ed-8bc3-aec76c41a181
grant_number: I05127
name: Evolutionary analysis of gene regulation
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/
record:
- id: '19294'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Pulsatile basal gene expression as a fitness determinant in bacteria
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19663'
abstract:
- lang: eng
text: The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules
that grow radially and exert forces on cargos. At the same time, mechanical stresses
from the microenvironment and cellular shape changes compress and bend microtubules.
Yet, centrosomes are membraneless organelles, raising the question of how centrosomes
withstand mechanical forces. Here, we discover that centrosomes can deform and
even fracture. We reveal that centrosomes experience deformations during navigational
pathfinding within motile cells. Coherence of the centrosome is maintained by
Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for
the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes
cellular function by generating coexisting microtubule organizing centers that
compete during path navigation and thereby cause cellular entanglement in the
microenvironment. Our findings show that cells actively maintain the integrity
of the centrosome to withstand mechanical forces. These results suggest that centrosome
stability preservation is fundamental, given that almost all cells in multicellular
organisms experience forces.
acknowledgement: "We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids;
M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP
plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang
for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids;
M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts,
Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating
DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for
providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and
F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support;
M. Benjamin Braun for critical reading of the manuscript; and the Core Facility
Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the
Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter
Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.);
German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation
grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent
within the framework of the German Excellence Initiative (J.R.); Medical & Clinician
Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG;
German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048
(D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag
457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.);
Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship
AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part
of the Excellence Strategy of the federal and state governments (E.K.); and CZI
grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from
the Chan Zuckerberg Initiative DAF (R.H.)."
article_number: eadx4047
article_processing_charge: Yes
article_type: original
author:
- first_name: Madeleine T.
full_name: Schmitt, Madeleine T.
last_name: Schmitt
- first_name: Janina
full_name: Kroll, Janina
last_name: Kroll
- first_name: Mauricio J.A.
full_name: Ruiz-Fernandez, Mauricio J.A.
last_name: Ruiz-Fernandez
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Shaunak
full_name: Ghosh, Shaunak
last_name: Ghosh
- first_name: Petra
full_name: Kameritsch, Petra
last_name: Kameritsch
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Johanna
full_name: Schmid, Johanna
last_name: Schmid
- first_name: Kasia
full_name: Stefanowski, Kasia
last_name: Stefanowski
- first_name: Andreas W.
full_name: Thomae, Andreas W.
last_name: Thomae
- first_name: Jingyuan
full_name: Cheng, Jingyuan
last_name: Cheng
- first_name: Gamze Naz
full_name: Öztan, Gamze Naz
last_name: Öztan
- first_name: Peter
full_name: Konopka, Peter
last_name: Konopka
- first_name: Germán Camargo
full_name: Ortega, Germán Camargo
last_name: Ortega
- first_name: Thomas
full_name: Penz, Thomas
last_name: Penz
- first_name: Luisa
full_name: Bach, Luisa
last_name: Bach
- first_name: Dirk
full_name: Baumjohann, Dirk
last_name: Baumjohann
- first_name: Christoph
full_name: Bock, Christoph
last_name: Bock
- first_name: Tobias
full_name: Straub, Tobias
last_name: Straub
- first_name: Felix
full_name: Meissner, Felix
last_name: Meissner
- first_name: Eva
full_name: Kiermaier, Eva
id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
last_name: Kiermaier
orcid: 0000-0001-6165-5738
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
citation:
ama: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from
fracturing enables efficient cell navigation. Science Advances. 2025;11(17).
doi:10.1126/sciadv.adx4047
apa: Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh,
S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing
enables efficient cell navigation. Science Advances. AAAS. https://doi.org/10.1126/sciadv.adx4047
chicago: Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert
Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes
from Fracturing Enables Efficient Cell Navigation.” Science Advances. AAAS,
2025. https://doi.org/10.1126/sciadv.adx4047.
ieee: M. T. Schmitt et al., “Protecting centrosomes from fracturing enables
efficient cell navigation,” Science Advances, vol. 11, no. 17. AAAS, 2025.
ista: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch
P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P,
Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier
E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient
cell navigation. Science Advances. 11(17), eadx4047.
mla: Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables
Efficient Cell Navigation.” Science Advances, vol. 11, no. 17, eadx4047,
AAAS, 2025, doi:10.1126/sciadv.adx4047.
short: M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P.
Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N.
Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub,
F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).
date_created: 2025-05-11T22:02:38Z
date_published: 2025-04-25T00:00:00Z
date_updated: 2025-09-30T12:26:21Z
day: '25'
ddc:
- '570'
department:
- _id: Bio
- _id: NanoFab
doi: 10.1126/sciadv.adx4047
external_id:
isi:
- '001476113400016'
pmid:
- '40279414'
file:
- access_level: open_access
checksum: e8ba22922fa5b23ccfcce8865f57226c
content_type: application/pdf
creator: dernst
date_created: 2025-05-12T07:46:10Z
date_updated: 2025-05-12T07:46:10Z
file_id: '19679'
file_name: 2025_ScienceAdvance_Schmitt.pdf
file_size: 2707050
relation: main_file
success: 1
file_date_updated: 2025-05-12T07:46:10Z
fulldoi: https://doi.org/10.1126/sciadv.adx4047
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '17'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protecting centrosomes from fracturing enables efficient cell navigation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 11
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20082'
abstract:
- lang: eng
text: Efficient immune responses rely on the capacity of leukocytes to traverse
diverse and complex tissues. To meet such changing environmental conditions, leukocytes
usually adopt an ameboid configuration, using their forward-positioned nucleus
as a probe to identify and follow the path of least resistance among pre-existing
pores. We show that, in dense environments where even the largest pores preclude
free passage, leukocytes position their nucleus behind the centrosome and organelles.
The local compression imposed on the cell body by its surroundings triggers assembly
of a central F-actin pool, located between cell front and nucleus. Central actin
pushes outward to transiently dilate a path for organelles and nucleus. Pools
of central and front actin are tightly coupled and experimental depletion of the
central pool enhances actin accumulation and protrusion formation at the cell
front. Although this shifted balance speeds up cells in permissive environments,
migration in restrictive environments is impaired, as the unleashed leading edge
dissociates from the trapped cell body. Our findings establish an actin regulatory
loop that balances path dilation with advancement of the leading edge to maintain
cellular coherence.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This research was supported by the Scientific Service Units of ISTA
through resources provided by the Imaging and Optics, Preclinical and Lab Support
Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus
Service Team, who helped generating the lentiviral particles used in this study.
We thank all the members of the Sixt group for valuable discussions and feedback,
in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing
the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback
during the writing of this manuscript. This work was supported by a European Research
Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral
Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian
Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical
Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB,
Kyushu University. Open access funding provided by Institute of Science and Technology
(IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Patricia
full_name: Dos Reis Rodrigues, Patricia
id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F
last_name: Dos Reis Rodrigues
orcid: 0000-0003-1681-508X
- first_name: Mario
full_name: Avellaneda Sarrió, Mario
id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E
last_name: Avellaneda Sarrió
orcid: 0000-0001-6406-524X
- first_name: Nikola
full_name: Canigova, Nikola
id: 3795523E-F248-11E8-B48F-1D18A9856A87
last_name: Canigova
orcid: 0000-0002-8518-5926
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
- first_name: Kari
full_name: Vaahtomeri, Kari
id: 368EE576-F248-11E8-B48F-1D18A9856A87
last_name: Vaahtomeri
orcid: 0000-0001-7829-3518
- first_name: Michael
full_name: Riedl, Michael
id: 3BE60946-F248-11E8-B48F-1D18A9856A87
last_name: Riedl
orcid: 0000-0003-4844-6311
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Yoshinori
full_name: Fukui, Yoshinori
last_name: Fukui
- first_name: Alba
full_name: Juanes Garcia, Alba
id: 40F05888-F248-11E8-B48F-1D18A9856A87
last_name: Juanes Garcia
orcid: 0000-0002-1009-9652
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune
cells globally coordinate protrusive forces. Nature Immunology. 2025;26:1258–1266.
doi:10.1038/s41590-025-02211-w
apa: Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R.,
Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally
coordinate protrusive forces. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-025-02211-w
chicago: Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova,
Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating
Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology.
Springer Nature, 2025. https://doi.org/10.1038/s41590-025-02211-w.
ieee: P. Dos Reis Rodrigues et al., “Migrating immune cells globally coordinate
protrusive forces,” Nature Immunology, vol. 26. Springer Nature, pp. 1258–1266,
2025.
ista: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri
K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt
MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature
Immunology. 26, 1258–1266.
mla: Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate
Protrusive Forces.” Nature Immunology, vol. 26, Springer Nature, 2025,
pp. 1258–1266, doi:10.1038/s41590-025-02211-w.
short: P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K.
Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes
Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.
corr_author: '1'
date_created: 2025-07-27T22:01:26Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-04-28T13:26:50Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/s41590-025-02211-w
external_id:
isi:
- '001529134300001'
pmid:
- '40664976'
file:
- access_level: open_access
checksum: 0c725123dca7797c682609bff2c4c5ac
content_type: application/pdf
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date_created: 2025-07-31T08:00:33Z
date_updated: 2025-07-31T08:00:33Z
file_id: '20096'
file_name: 2025_NatureImmunology_ReisRodrigues.pdf
file_size: 13514646
relation: main_file
success: 1
file_date_updated: 2025-07-31T08:00:33Z
fulldoi: https://doi.org/10.1038/s41590-025-02211-w
has_accepted_license: '1'
intvolume: ' 26'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 1258–1266
pmid: 1
project:
- _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd
grant_number: '101071793'
name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal
pushing forces'
- _id: c092d618-5a5b-11eb-8a69-f92e1e843fc8
grant_number: 944-2020
name: 'Bioelectric patrolling: the role of the local membrane potential in immune
cell migration'
publication: Nature Immunology
publication_identifier:
eissn:
- 1529-2916
issn:
- 1529-2908
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/bench-pressing-cells/
record:
- id: '20149'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Migrating immune cells globally coordinate protrusive forces
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 26
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '17468'
abstract:
- lang: eng
text: Oxygen redox chemistry is central to life1 and many human-made technologies,
such as in energy storage2,3,4. The large energy gain from oxygen redox reactions
is often connected with the occurrence of harmful reactive oxygen species3,5,6.
Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which
may evolve from superoxide. However, the factors determining the formation of
singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown.
Here we report that the release of triplet or singlet oxygen is governed by individual
Marcus normal and inverted region behaviour. We found that as the driving force
for the reaction increases, the initially dominant evolution of triplet oxygen
slows down, and singlet oxygen evolution becomes predominant with higher maximum
kinetics. This behaviour also applies to the widely observed superoxide disproportionation,
in which one superoxide is oxidized by another, in both non-aqueous and aqueous
systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet
oxygen yields governed by these conditions are relevant, for example, in batteries
or cellular organelles in which superoxide forms. Our findings suggest ways to
understand and control spin states and kinetics in oxygen redox chemistry, with
implications for fields, including life sciences, pure chemistry and energy storage.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: M-Shop
- _id: ScienComp
acknowledgement: S.A.F. thanks the Institute of Science and Technology Austria (ISTA)
for the support. The Scientific Service Units of ISTA supported this research through
resources provided by the Imaging and Optics Facility, the Lab Support Facility,
the Miba Machine Shop and Scientific Computing. This research was partly funded
by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open
access purposes, the author has applied for a CC BY public copyright licence to
any author-accepted manuscript version arising from this submission. R.H. acknowledges
funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg
Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989).
H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters
programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life
and R. Jethwa for proofreading. Open access funding was provided by ISTA.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Soumyadip
full_name: Mondal, Soumyadip
id: d25d21ef-dc8d-11ea-abe3-ec4576307f48
last_name: Mondal
- first_name: Huyen T.K.
full_name: Nguyen, Huyen T.K.
last_name: Nguyen
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Stefan Alexander
full_name: Freunberger, Stefan Alexander
id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425
last_name: Freunberger
orcid: 0000-0003-2902-5319
citation:
ama: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control
singlet and triplet oxygen evolving from superoxide. Nature. 2025;646(8085):601–605.
doi:10.1038/s41586-025-09587-7
apa: Mondal, S., Nguyen, H. T. K., Hauschild, R., & Freunberger, S. A. (2025).
Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature.
Springer Nature. https://doi.org/10.1038/s41586-025-09587-7
chicago: Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander
Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from
Superoxide.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-09587-7.
ieee: S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics
control singlet and triplet oxygen evolving from superoxide,” Nature, vol.
646, no. 8085. Springer Nature, pp. 601–605, 2025.
ista: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control
singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605.
mla: Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen
Evolving from Superoxide.” Nature, vol. 646, no. 8085, Springer Nature,
2025, pp. 601–605, doi:10.1038/s41586-025-09587-7.
short: S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025)
601–605.
corr_author: '1'
date_created: 2024-08-29T10:40:23Z
date_published: 2025-10-16T00:00:00Z
date_updated: 2026-09-16T06:53:54Z
day: '16'
ddc:
- '540'
department:
- _id: StFr
- _id: Bio
doi: 10.1038/s41586-025-09587-7
external_id:
isi:
- '001586378900001'
pmid:
- '41044415'
file:
- access_level: open_access
checksum: b507ddd23df0388aa65d04dc9b00fe3d
content_type: application/pdf
creator: dernst
date_created: 2025-10-20T10:26:13Z
date_updated: 2025-10-20T10:26:13Z
file_id: '20500'
file_name: 2025_Nature_Mondal.pdf
file_size: 3809247
relation: main_file
success: 1
file_date_updated: 2025-10-20T10:26:13Z
fulldoi: https://doi.org/10.1038/s41586-025-09587-7
has_accepted_license: '1'
intvolume: ' 646'
isi: 1
issue: '8085'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 601–605
pmid: 1
project:
- _id: 8df062be-16d5-11f0-9cad-f559b6612c7e
grant_number: P37169
name: Singlet oxygen in non-aqueous oxygen redox chemistry
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
- _id: 5eaf4378-b033-11f1-b276-f928018a46c1
grant_number: COE05
name: Materials for Energy Conversion and Storage (Freunberger)
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/taming-the-bad-oxygen/
scopus_import: '1'
status: public
title: Marcus kinetics control singlet and triplet oxygen evolving from superoxide
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 646
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19704'
abstract:
- lang: eng
text: The information-processing capability of the brain’s cellular network depends
on the physical wiring pattern between neurons and their molecular and functional
characteristics. Mapping neurons and resolving their individual synaptic connections
can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular
labelling. Light microscopy is uniquely positioned to visualize specific molecules,
but dense, synapse-level circuit reconstruction by light microscopy has been out
of reach, owing to limitations in resolution, contrast and volumetric imaging
capability. Here we describe light-microscopy-based connectomics (LICONN). We
integrated specifically engineered hydrogel embedding and expansion with comprehensive
deep-learning-based segmentation and analysis of connectivity, thereby directly
incorporating molecular information into synapse-level reconstructions of brain
tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological
experiments in a readily adoptable manner.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
- _id: PreCl
- _id: M-Shop
- _id: E-Lib
acknowledgement: 'We thank S. Dorkenwald and P. Li for critical reading of the manuscript,
S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge
support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific
Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge
funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D.
and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft
für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s
Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions
Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation
programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open
access funding provided by Institute of Science and Technology (IST Austria).'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mojtaba
full_name: Tavakoli, Mojtaba
id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87
last_name: Tavakoli
orcid: 0000-0002-7667-6854
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Michał
full_name: Januszewski, Michał
last_name: Januszewski
- first_name: Vitali
full_name: Vistunou, Vitali
id: 7e146587-8972-11ed-ae7b-d7a32ea86a81
last_name: Vistunou
- first_name: Nathalie
full_name: Agudelo Duenas, Nathalie
id: 40E7F008-F248-11E8-B48F-1D18A9856A87
last_name: Agudelo Duenas
- first_name: Jakob
full_name: Vorlaufer, Jakob
id: 937696FA-C996-11E9-8C7C-CF13E6697425
last_name: Vorlaufer
orcid: 0009-0000-7590-3501
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Bárbara
full_name: Oliveira, Bárbara
id: 3B03AA1A-F248-11E8-B48F-1D18A9856A87
last_name: Oliveira
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Viren
full_name: Jain, Viren
last_name: Jain
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic
reconstruction of mammalian brain tissue. Nature. 2025;642:398-410. doi:10.1038/s41586-025-08985-1
apa: Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas,
N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction
of mammalian brain tissue. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08985-1
chicago: Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou,
Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based
Connectomic Reconstruction of Mammalian Brain Tissue.” Nature. Springer
Nature, 2025. https://doi.org/10.1038/s41586-025-08985-1.
ieee: M. Tavakoli et al., “Light-microscopy-based connectomic reconstruction
of mammalian brain tissue,” Nature, vol. 642. Springer Nature, pp. 398–410,
2025.
ista: Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer
J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl
JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain
tissue. Nature. 642, 398–410.
mla: Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction
of Mammalian Brain Tissue.” Nature, vol. 642, Springer Nature, 2025, pp.
398–410, doi:10.1038/s41586-025-08985-1.
short: M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas,
J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino,
V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.
corr_author: '1'
date_created: 2025-05-18T22:02:51Z
date_published: 2025-06-12T00:00:00Z
date_updated: 2026-10-02T09:59:44Z
day: '12'
ddc:
- '570'
department:
- _id: JoDa
- _id: GradSch
- _id: Bio
- _id: GaNo
doi: 10.1038/s41586-025-08985-1
ec_funded: 1
external_id:
isi:
- '001483477000001'
pmid:
- '40335689'
file:
- access_level: open_access
checksum: ebc99d7108e728f46db0a009292675ef
content_type: application/pdf
creator: dernst
date_created: 2025-07-03T06:55:20Z
date_updated: 2025-07-03T06:55:20Z
file_id: '19959'
file_name: 2025_Nature_Tavakoli.pdf
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success: 1
file_date_updated: 2025-07-03T06:55:20Z
fulldoi: https://doi.org/10.1038/s41586-025-08985-1
has_accepted_license: '1'
intvolume: ' 642'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 398-410
pmid: 1
project:
- _id: 6285a163-2b32-11ec-9570-8e204ca2dba5
grant_number: '26137'
name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion
Microscopy
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6
grant_number: '101044865'
name: Toward an understanding of the brain interstitial system and the extracellular
proteome in health and autism spectrum disorders
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/
record:
- id: '18697'
relation: research_data
status: public
- id: '18677'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Light-microscopy-based connectomic reconstruction of mammalian brain tissue
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 642
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19404'
abstract:
- lang: eng
text: Cell migration is a fundamental process during embryonic development. Most
studies in vivo have focused on the migration of cells using the extracellular
matrix (ECM) as their substrate for migration. In contrast, much less is known
about how cells migrate on other cells, as found in early embryos when the ECM
has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the
early zebrafish gastrula use the ectoderm as their substrate for migration. We
show that the lateral ectoderm is permissive for the animal-pole-directed migration
of LME cells, while the ectoderm at the animal pole halts it. These differences
in permissiveness depend on the lateral ectoderm being more cohesive than the
animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling
within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion
as one critical factor in regulating LME migration during zebrafish gastrulation.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
acknowledgement: 'We are grateful to the colleagues who contributed to this work with
discussions, technical advice, and feedback on the manuscript: Irene Steccari, David
Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian
Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the
Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing
Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter
Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was
supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral
Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship
(ALTF 1159-2018).'
article_number: '115387'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ste
full_name: Tavano, Ste
id: 2F162F0C-F248-11E8-B48F-1D18A9856A87
last_name: Tavano
orcid: 0000-0001-9970-7804
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Xin
full_name: Tong, Xin
id: 50F65CDC-AA30-11E9-A72B-8A12E6697425
last_name: Tong
- first_name: Roland
full_name: Kardos, Roland
id: 4039350E-F248-11E8-B48F-1D18A9856A87
last_name: Kardos
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm
tissue material properties modulates lateral mesendoderm cell migration during
early zebrafish gastrulation. Cell Reports. 2025;44(3). doi:10.1016/j.celrep.2025.115387
apa: Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A.,
… Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material
properties modulates lateral mesendoderm cell migration during early zebrafish
gastrulation. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2025.115387
chicago: Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra
Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning
of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration
during Early Zebrafish Gastrulation.” Cell Reports. Cell Press, 2025. https://doi.org/10.1016/j.celrep.2025.115387.
ieee: S. Tavano et al., “BMP-dependent patterning of ectoderm tissue material
properties modulates lateral mesendoderm cell migration during early zebrafish
gastrulation,” Cell Reports, vol. 44, no. 3. Cell Press, 2025.
ista: Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R,
Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties
modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
Cell Reports. 44(3), 115387.
mla: Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties
Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.”
Cell Reports, vol. 44, no. 3, 115387, Cell Press, 2025, doi:10.1016/j.celrep.2025.115387.
short: S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild,
C.-P.J. Heisenberg, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-03-16T23:01:24Z
date_published: 2025-03-25T00:00:00Z
date_updated: 2026-10-20T08:20:45Z
day: '25'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MiSi
- _id: Bio
doi: 10.1016/j.celrep.2025.115387
external_id:
isi:
- '001443652700001'
pmid:
- '40057955'
file:
- access_level: open_access
checksum: 57e05dd1598c807af0afdb32cec039d3
content_type: application/pdf
creator: dernst
date_created: 2025-03-17T10:26:54Z
date_updated: 2025-03-17T10:26:54Z
file_id: '19413'
file_name: 2025_CellReports_Tavano.pdf
file_size: 9067797
relation: main_file
success: 1
file_date_updated: 2025-03-17T10:26:54Z
fulldoi: https://doi.org/10.1016/j.celrep.2025.115387
has_accepted_license: '1'
intvolume: ' 44'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
- _id: 269CD5C4-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1159-2018
name: 'Mechanosensation in cell migration: the role of friction forces in cell polarization
and directed migration'
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral
mesendoderm cell migration during early zebrafish gastrulation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '19520'
abstract:
- lang: eng
text: Vertebrates exhibit a wide range of motor behaviors, ranging from swimming
to complex limb-based movements. Here we take advantage of frog metamorphosis,
which captures a swim-to-limb-based movement transformation during the development
of a single organism, to explore changes in the underlying spinal circuits. We
find that the tadpole spinal cord contains small and largely homogeneous populations
of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages.
These neuronal populations only modestly increase in number and subtype heterogeneity
with the emergence of free swimming. In contrast, during frog metamorphosis and
the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron
number and transcriptional heterogeneity, culminating in cohorts of neurons that
exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated
gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively,
results in severe but selective deficits in tail and limb function. Our work thus
demonstrates that neural diversity scales exponentially with increasing behavioral
complexity and illustrates striking evolutionary conservation in the molecular
organization and function of motor circuits across species.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We would like to thank the members of the Sweeney Lab (especially
Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project
and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion,
and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their
scientific insight and mentorship during the conception of this project. This\r\nproject
would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena
Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA).
In addition, we thank our funding sources for providing the resources to do these\r\nexperiments:
FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon
Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch
Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS
5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.);
NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities
(ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472
(Z.H.); and Project A.L.S. (S.B-M.). "
article_processing_charge: No
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales
exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv.
doi:10.1101/2024.09.20.614050
apa: Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R.,
Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis. bioRxiv. https://doi.org/10.1101/2024.09.20.614050
chicago: Vijatovic, David, Florina Alexandra Toma, Zoe P Harrington, Christoph
M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal
Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during
Frog Metamorphosis.” BioRxiv, n.d. https://doi.org/10.1101/2024.09.20.614050.
ieee: D. Vijatovic et al., “Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis,” bioRxiv. .
ista: Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ,
Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney
LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis. bioRxiv, 10.1101/2024.09.20.614050.
mla: Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with
Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, doi:10.1101/2024.09.20.614050.
short: D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J.
Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen,
J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2025-04-07T08:48:28Z
date_published: 2024-09-27T00:00:00Z
date_updated: 2025-05-14T11:40:13Z
day: '27'
department:
- _id: LoSw
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1101/2024.09.20.614050
fulldoi: https://doi.org/10.1101/2024.09.20.614050
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.09.20.614050
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: bioRxiv
publication_status: submitted
status: public
title: Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '15048'
abstract:
- lang: eng
text: Embryogenesis results from the coordinated activities of different signaling
pathways controlling cell fate specification and morphogenesis. In vertebrate
gastrulation, both Nodal and BMP signaling play key roles in germ layer specification
and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis
is still insufficiently understood. Here, we took a reductionist approach using
zebrafish embryonic explants to study the coordination of Nodal and BMP signaling
for embryo patterning and morphogenesis. We show that Nodal signaling triggers
explant elongation by inducing mesendodermal progenitors but also suppressing
BMP signaling activity at the site of mesendoderm induction. Consistent with this,
ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm
intercalations, key processes during explant elongation. Translating these ex
vivo observations to the intact embryo showed that, similar to explants, Nodal
signaling suppresses the effect of BMP signaling on cell intercalations in the
dorsal domain, thus allowing robust embryonic axis elongation. These findings
suggest a dual function of Nodal signaling in embryonic axis elongation by both
inducing mesendoderm and suppressing BMP effects in the dorsal portion of the
mesendoderm.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Patrick Müller for sharing the chordintt250 mutant zebrafish
line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b
plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated
the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro
and Katherine Rogers and members of the Heisenberg lab for discussions, technical
advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo
for discussions. We thank the Imaging and Optics Facility as well as the Life Science
facility at IST Austria for support with microscopy and fish maintenance.\r\nThis
work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573
to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy
of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience
and Technology Austria. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Kornelija
full_name: Pranjic-Ferscha, Kornelija
id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
last_name: Pranjic-Ferscha
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation
by Nodal-dependent restriction of BMP signaling. Development. 2024;151(4):1-18.
doi:10.1242/dev.202316
apa: Schauer, A., Pranjic-Ferscha, K., Hauschild, R., & Heisenberg, C.-P. J.
(2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling.
Development. Company of Biologists. https://doi.org/10.1242/dev.202316
chicago: Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp
J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.”
Development. Company of Biologists, 2024. https://doi.org/10.1242/dev.202316.
ieee: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust
axis elongation by Nodal-dependent restriction of BMP signaling,” Development,
vol. 151, no. 4. Company of Biologists, pp. 1–18, 2024.
ista: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis
elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4),
1–18.
mla: Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction
of BMP Signaling.” Development, vol. 151, no. 4, Company of Biologists,
2024, pp. 1–18, doi:10.1242/dev.202316.
short: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development
151 (2024) 1–18.
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: A custom MATLAB script for analyzing cell dispersal has
been deposited in the ISTA library (doi:10.15479/AT:ISTA:14926)
date_created: 2024-03-03T23:00:50Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2026-10-01T08:32:34Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1242/dev.202316
ec_funded: 1
external_id:
isi:
- '001170580200001'
pmid:
- '38372390'
file:
- access_level: open_access
checksum: 6961ea10012bf0d266681f9628bb8f13
content_type: application/pdf
creator: dernst
date_created: 2024-03-04T07:24:43Z
date_updated: 2024-03-04T07:24:43Z
file_id: '15050'
file_name: 2024_Development_Schauer.pdf
file_size: 14839986
relation: main_file
success: 1
file_date_updated: 2024-03-04T07:24:43Z
fulldoi: https://doi.org/10.1242/dev.202316
has_accepted_license: '1'
intvolume: ' 151'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1-18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14926'
relation: research_data
status: public
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Robust axis elongation by Nodal-dependent restriction of BMP signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 151
year: '2024'
...