[{"_id":"21383","month":"02","title":"Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians","article_processing_charge":"Yes","citation":{"mla":"Sasidharan, Vidyanand, et al. “Extracellular Vesicles Mediate Stem Cell Signaling and Systemic RNAi in Planarians.” <i>Science Advances</i>, vol. 12, no. 6, eady1461, American Association for the Advancement of Science, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.ady1461\">10.1126/sciadv.ady1461</a>.","short":"V. Sasidharan, L. Ancellotti, V. Doddihal, C. Brewster, F. Mann, M.C. McKinney, J. Varberg, E. Ross, F. Deng, K. Yi, A. Sánchez Alvarado, Science Advances 12 (2026).","ieee":"V. Sasidharan <i>et al.</i>, “Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians,” <i>Science Advances</i>, vol. 12, no. 6. American Association for the Advancement of Science, 2026.","apa":"Sasidharan, V., Ancellotti, L., Doddihal, V., Brewster, C., Mann, F., McKinney, M. C., … Sánchez Alvarado, A. (2026). Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.ady1461\">https://doi.org/10.1126/sciadv.ady1461</a>","ama":"Sasidharan V, Ancellotti L, Doddihal V, et al. Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. <i>Science Advances</i>. 2026;12(6). doi:<a href=\"https://doi.org/10.1126/sciadv.ady1461\">10.1126/sciadv.ady1461</a>","ista":"Sasidharan V, Ancellotti L, Doddihal V, Brewster C, Mann F, McKinney MC, Varberg J, Ross E, Deng F, Yi K, Sánchez Alvarado A. 2026. Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. Science Advances. 12(6), eady1461.","chicago":"Sasidharan, Vidyanand, Laura Ancellotti, Viraj Doddihal, Carolyn Brewster, Frederick Mann, Mary Cathleen McKinney, Joseph Varberg, et al. “Extracellular Vesicles Mediate Stem Cell Signaling and Systemic RNAi in Planarians.” <i>Science Advances</i>. American Association for the Advancement of Science, 2026. <a href=\"https://doi.org/10.1126/sciadv.ady1461\">https://doi.org/10.1126/sciadv.ady1461</a>."},"ddc":["570"],"OA_type":"gold","article_type":"original","OA_place":"publisher","doi":"10.1126/sciadv.ady1461","status":"public","tmp":{"short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"issue":"6","publisher":"American Association for the Advancement of Science","type":"journal_article","day":"01","date_published":"2026-02-01T00:00:00Z","has_accepted_license":"1","date_updated":"2026-03-02T14:23:22Z","year":"2026","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":12,"quality_controlled":"1","article_number":"eady1461","license":"https://creativecommons.org/licenses/by-nc/4.0/","abstract":[{"lang":"eng","text":"Planarian flatworms are known for their remarkable regenerative capacity; however, the precise intercellular communication mechanisms underlying this process remain unsolved. Here, we report the discovery and characterization of abundant extracellular vesicles (EVs) in planarians. Using imaging and molecular analysis, we show conservation of biogenesis, morphology, and protein composition of planarian EVs. Environmental stressors significantly elevate EV release, indicating that planarians dynamically regulate vesicle production. Functionally, planarian EVs mediate intercellular communication by transferring regulatory signals: We find that they shuttle small RNAs that effect systemic RNA interference (RNAi) throughout the organism. Notably, gene knockdown experiments reveal a crucial role for AGO-3, a member of the Argonaute family of proteins, in modulating the association of small interfering RNAs with EVs, linking the intracellular RNAi machinery to EV-based signaling. These findings highlight EVs as pivotal mediators of cell-cell communication in planarians, with broad implications for understanding the coordination of gene regulation and tissue regeneration in animals."}],"publication_status":"published","date_created":"2026-03-02T10:08:07Z","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"acknowledgement":"We thank all the Sánchez Alvarado lab members for inputs and discussions. We are grateful to the Stowers Aquatics (particularly the Planarian team), Microscopy, and Molecular Biology core facilities for technical contributions and method development; e. n. lissek and A. Fujii from Oni US and S. Wang from the University of Missouri, Kansas city, for assistance with dStORM imaging; and d. Alburty and A. Page from innovaprep for assisting with the ntA. We also thank M. Miller for the illustrations. This work was supported by the hhMi and Stowers institute. ","file":[{"access_level":"open_access","relation":"main_file","success":1,"date_updated":"2026-03-02T14:19:35Z","content_type":"application/pdf","checksum":"fa9f6dafe3538e2d2872c098e06d1712","file_size":2841345,"creator":"dernst","file_name":"2026_ScienceAdv_Sasidharan.pdf","file_id":"21389","date_created":"2026-03-02T14:19:35Z"}],"DOAJ_listed":"1","publication_identifier":{"eissn":["2375-2548"]},"oa":1,"scopus_import":"1","author":[{"first_name":"Vidyanand","full_name":"Sasidharan, Vidyanand","last_name":"Sasidharan"},{"first_name":"Laura","full_name":"Ancellotti, Laura","last_name":"Ancellotti"},{"id":"034e0824-174b-11ef-b32b-9366a0e70d1c","first_name":"Viraj","full_name":"Doddihal, Viraj","last_name":"Doddihal"},{"full_name":"Brewster, Carolyn","last_name":"Brewster","first_name":"Carolyn"},{"full_name":"Mann, Frederick","last_name":"Mann","first_name":"Frederick"},{"last_name":"McKinney","full_name":"McKinney, Mary Cathleen","first_name":"Mary Cathleen"},{"first_name":"Joseph","last_name":"Varberg","full_name":"Varberg, Joseph"},{"last_name":"Ross","full_name":"Ross, Eric","first_name":"Eric"},{"full_name":"Deng, Fengyan","last_name":"Deng","first_name":"Fengyan"},{"first_name":"Kexi","last_name":"Yi","full_name":"Yi, Kexi"},{"first_name":"Alejandro","full_name":"Sánchez Alvarado, Alejandro","last_name":"Sánchez Alvarado"}],"file_date_updated":"2026-03-02T14:19:35Z","publication":"Science Advances","intvolume":"        12"},{"oa_version":"None","year":"2026","date_updated":"2026-03-24T08:32:00Z","project":[{"name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"name":"Keratins in epithelial tissue spreading","_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023"},{"grant_number":"W1250-B20","_id":"252C3B08-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF"}],"has_accepted_license":"1","date_published":"2026-03-24T00:00:00Z","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","tmp":{"short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png"},"status":"public","type":"research_data","publisher":"Institute of Science and Technology Austria","day":"24","doi":"10.15479/AT-ISTA-21137","OA_place":"repository","OA_type":"free access","title":"Data associated with Keratins coordinate tissue spreading ","month":"3","_id":"21137","citation":{"ieee":"S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute of Science and Technology Austria, 2026.","short":"S. Naik, (2026).","mla":"Naik, Suyash. <i>Data Associated with Keratins Coordinate Tissue Spreading </i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","ista":"Naik S. 2026. Data associated with Keratins coordinate tissue spreading , Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","chicago":"Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>.","apa":"Naik, S. (2026). Data associated with Keratins coordinate tissue spreading . Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>","ama":"Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>"},"article_processing_charge":"No","ec_funded":1,"author":[{"full_name":"Naik, Suyash","last_name":"Naik","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash","orcid":"0000-0001-8421-5508"}],"file_date_updated":"2026-03-24T07:21:43Z","oa":1,"acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","file":[{"title":"Cell git repository","access_level":"open_access","relation":"main_file","date_updated":"2026-03-16T11:51:10Z","content_type":"application/zip","checksum":"5d1fda7e410f24c311fcf6bcf725698f","file_size":725916,"description":"Python3 library written in C++20 to integrate vertex models. Please read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed instructions for installation and usage of the code in this repository. ","creator":"snaik","file_name":"cells-main.zip","file_id":"21461","date_created":"2026-03-16T11:51:10Z"},{"access_level":"open_access","relation":"main_file","success":1,"content_type":"application/x-zip-compressed","checksum":"ee350c8eaed99f3ca348c47c8b190d3c","date_updated":"2026-03-18T14:52:02Z","creator":"snaik","file_name":"DevBranchDataRepo.zip","date_created":"2026-03-18T14:52:02Z","file_id":"21464","file_size":282168895},{"checksum":"1ecaf2c1a2ce8ff9c75a128cc02d0b8f","content_type":"text/markdown","date_updated":"2026-03-18T15:01:32Z","file_name":"ReadMe.md","creator":"snaik","file_id":"21466","date_created":"2026-03-18T15:01:32Z","file_size":2231,"access_level":"open_access","relation":"main_file","success":1},{"content_type":"image/svg+xml","checksum":"da9a4687e5144b61a64ca341f922046a","date_updated":"2026-03-18T15:12:57Z","date_created":"2026-03-18T15:12:57Z","file_id":"21467","file_name":"PaperSchematics.svg","creator":"snaik","file_size":1951210,"relation":"main_file","access_level":"open_access","success":1},{"date_updated":"2026-03-21T03:37:43Z","content_type":"application/octet-stream","checksum":"9ac1054b16c212c6f34d402dce2c80e0","file_size":1897,"date_created":"2026-03-21T03:37:43Z","file_id":"21468","file_name":"maxwell_sketch.tex","creator":"snaik","relation":"main_file","access_level":"open_access","success":1},{"relation":"main_file","access_level":"open_access","success":1,"date_updated":"2026-03-24T07:21:43Z","content_type":"application/x-zip-compressed","checksum":"7c9ecf78e2593b3830d96fa94baa08df","file_size":749368723,"date_created":"2026-03-24T07:21:43Z","file_id":"21495","file_name":"DataRepo.zip","creator":"snaik"}],"corr_author":"1","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"contributor":[{"last_name":"Keta","contributor_type":"researcher","first_name":"Yann-Edwin"},{"contributor_type":"supervisor","last_name":"Henkes","first_name":"Silke "},{"orcid":"0000-0002-0912-4566","contributor_type":"supervisor","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-6005-1561","contributor_type":"supervisor","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"date_created":"2026-02-04T16:38:02Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"license":"https://creativecommons.org/licenses/by-sa/4.0/"},{"publication_identifier":{"issnl":[" 1745-2473"],"eissn":["1745-2481"],"issn":["1745-2473"]},"oa":1,"acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","file":[{"checksum":"0ab7ac2fbcb61a364dba57152db64ed7","content_type":"application/pdf","date_updated":"2026-01-21T08:21:11Z","creator":"dernst","file_name":"2026_NaturePhysics_Mishra.pdf","date_created":"2026-01-21T08:21:11Z","file_id":"21026","file_size":7335694,"access_level":"open_access","relation":"main_file","success":1}],"ec_funded":1,"intvolume":"        22","publication":"Nature Physics","author":[{"last_name":"Mishra","full_name":"Mishra, Nikhil","first_name":"Nikhil","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","orcid":"0000-0002-6425-5788"},{"id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","first_name":"Yuting I","last_name":"Li","full_name":"Li, Yuting I"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"file_date_updated":"2026-01-21T08:21:11Z","scopus_import":"1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"license":"https://creativecommons.org/licenses/by/4.0/","abstract":[{"lang":"eng","text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning."}],"quality_controlled":"1","volume":22,"oaworkid":1,"corr_author":"1","language":[{"iso":"eng"}],"external_id":{"oaworkid":["W7118187193"]},"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"date_created":"2026-01-20T10:12:19Z","publication_status":"published","type":"journal_article","publisher":"Springer Nature","day":"05","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","page":"139-150","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","year":"2026","oa_version":"Published Version","date_updated":"2026-04-28T12:55:30Z","project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"},{"_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020"},{"_id":"917c023a-16d5-11f0-9cad-eb5cafc52090","name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development"}],"has_accepted_license":"1","date_published":"2026-01-05T00:00:00Z","citation":{"ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>"},"PlanS_conform":"1","related_material":{"link":[{"description":"News on ISTA website","relation":"research_data","url":"https://ista.ac.at/en/news/geometry-shapes-life/"}]},"article_processing_charge":"Yes (via OA deal)","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","month":"01","_id":"21015","doi":"10.1038/s41567-025-03122-1","OA_place":"publisher","article_type":"original","OA_type":"hybrid","ddc":["570"]},{"oa":1,"publication_identifier":{"eissn":["1477-9129"],"issn":["0950-1991"]},"pmid":1,"file":[{"relation":"main_file","access_level":"open_access","success":1,"date_updated":"2025-07-23T08:43:01Z","content_type":"application/pdf","checksum":"808d8aa28df79d23fb661838d1fdc1be","file_size":25935563,"date_created":"2025-07-23T08:43:01Z","file_id":"20070","creator":"dernst","file_name":"2025_Development_Moriyama.pdf"}],"acknowledgement":"We thank all the Heisenberg lab members for discussions and comments on the manuscript, and the Bioimaging and Life Science facilities of ISTA for support with microscopy and fish maintenance, respectively. This study was funded by a Japan Society for the Promotion of Science (JSPS) Overseas Research Fellowship and a Japan Science and Technology Agency PRESTO grant (JPMJPR214B) to Y.M. Open Access funding provided by the Japan Science and Technology Agency. Deposited in PMC for immediate release.","isi":1,"intvolume":"       152","file_date_updated":"2025-07-23T08:43:01Z","publication":"Development","author":[{"full_name":"Moriyama, Yuuta","last_name":"Moriyama","id":"addc9b8c-67a0-11f0-b374-a2e094825470","first_name":"Yuuta","orcid":"0000-0002-2853-8051"},{"first_name":"Toshiyuki","full_name":"Mitsui, Toshiyuki","last_name":"Mitsui"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":"1","quality_controlled":"1","volume":152,"article_number":"dev204261","abstract":[{"lang":"eng","text":"During embryonic development, cell behaviors need to be tightly regulated in time and space. Yet how the temporal and spatial regulations of cell behaviors are interconnected during embryonic development remains elusive. To address this, we turned to zebrafish gastrulation, the process whereby dynamic cell behaviors generate the three principal germ layers of the early embryo. Here, we show that Hoxb cluster genes are expressed in a temporally collinear manner at the blastoderm margin, where mesodermal and endodermal (mesendoderm) progenitor cells are specified and ingress to form mesendoderm/hypoblast. Functional analysis shows that these Hoxb genes regulate the timing of cell ingression: under- or overexpression of Hoxb genes perturb the timing of mesendoderm cell ingression and, consequently, the positioning of these cells along the forming anterior-posterior body axis after gastrulation. Finally, we found that Hoxb genes control the timing of mesendoderm ingression by regulating cellular bleb formation and cell surface fluctuations in the ingressing cells. Collectively, our findings suggest that Hoxb genes interconnect the temporal and spatial pattern of cell behaviors during zebrafish gastrulation by controlling cell surface fluctuations."}],"publication_status":"published","language":[{"iso":"eng"}],"corr_author":"1","date_created":"2025-07-21T08:10:32Z","external_id":{"pmid":["40576478"],"isi":["001525252300001"]},"department":[{"_id":"CaHe"}],"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","issue":"12","publisher":"The Company of Biologists","day":"27","type":"journal_article","date_updated":"2025-09-30T14:07:51Z","oa_version":"Published Version","year":"2025","date_published":"2025-06-27T00:00:00Z","has_accepted_license":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"06","title":"Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation","_id":"20048","PlanS_conform":"1","citation":{"ista":"Moriyama Y, Mitsui T, Heisenberg C-PJ. 2025. Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. Development. 152(12), dev204261.","chicago":"Moriyama, Yuuta, Toshiyuki Mitsui, and Carl-Philipp J Heisenberg. “Hoxb Genes Determine the Timing of Cell Ingression by Regulating Cell Surface Fluctuations during Zebrafish Gastrulation.” <i>Development</i>. The Company of Biologists, 2025. <a href=\"https://doi.org/10.1242/dev.204261\">https://doi.org/10.1242/dev.204261</a>.","apa":"Moriyama, Y., Mitsui, T., &#38; Heisenberg, C.-P. J. (2025). Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.204261\">https://doi.org/10.1242/dev.204261</a>","ama":"Moriyama Y, Mitsui T, Heisenberg C-PJ. Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. <i>Development</i>. 2025;152(12). doi:<a href=\"https://doi.org/10.1242/dev.204261\">10.1242/dev.204261</a>","ieee":"Y. Moriyama, T. Mitsui, and C.-P. J. Heisenberg, “Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation,” <i>Development</i>, vol. 152, no. 12. The Company of Biologists, 2025.","short":"Y. Moriyama, T. Mitsui, C.-P.J. Heisenberg, Development 152 (2025).","mla":"Moriyama, Yuuta, et al. “Hoxb Genes Determine the Timing of Cell Ingression by Regulating Cell Surface Fluctuations during Zebrafish Gastrulation.” <i>Development</i>, vol. 152, no. 12, dev204261, The Company of Biologists, 2025, doi:<a href=\"https://doi.org/10.1242/dev.204261\">10.1242/dev.204261</a>."},"article_processing_charge":"Yes (via OA deal)","ddc":["570"],"article_type":"original","OA_type":"hybrid","OA_place":"publisher","doi":"10.1242/dev.204261"},{"acknowledgement":"We thank members of the Conradt lab, the Center for Cell and Molecular Dynamics (https://www.uclccmd.co.uk/) and T. Schedl for discussions and comments on the manuscript. We thank L. McGuinness for excellent technical support. Some strains were provided by the Caenorhabditis Genetics Center (CGC), which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We thank Alex Hajnal (University of Zurich, Switzerland) and Andrew deMello (ETH Zurich, Switzerland) for their support of S.B. This work was supported by a predoctoral fellowship from the Studienstiftung des deutschen Volkes to NM, funds from UCL (Division of Biosciences, UCL LSM Capital Equipment Fund) to B.C., and a Wolfson Fellowship from the Royal Society (https://royalsociety.org/) to B.C. (RSWF\\R1\\180008), and the Biotechnology and Biological Sciences Research Council (https://bbsrc.ukri.org/) (BB/V007572/1 and BB/V015648/1to B.C.).","DOAJ_listed":"1","file":[{"relation":"main_file","access_level":"open_access","success":1,"content_type":"application/pdf","checksum":"f28e73963ea1f55876d0d1afca0f706a","date_updated":"2025-09-01T09:46:44Z","file_id":"20261","date_created":"2025-09-01T09:46:44Z","creator":"dernst","file_name":"2025_NatureComm_Segos.pdf","file_size":3775190}],"pmid":1,"oa":1,"publication_identifier":{"eissn":["2041-1723"]},"scopus_import":"1","author":[{"first_name":"Ioannis","last_name":"Segos","full_name":"Segos, Ioannis"},{"first_name":"Jens","full_name":"Van Eeckhoven, Jens","last_name":"Van Eeckhoven"},{"full_name":"Berger, Simon","last_name":"Berger","first_name":"Simon"},{"id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","first_name":"Nikhil","last_name":"Mishra","full_name":"Mishra, Nikhil","orcid":"0000-0002-6425-5788"},{"first_name":"Eric J.","last_name":"Lambie","full_name":"Lambie, Eric J."},{"first_name":"Barbara","last_name":"Conradt","full_name":"Conradt, Barbara"}],"publication":"Nature Communications","file_date_updated":"2025-09-01T09:46:44Z","intvolume":"        16","abstract":[{"lang":"eng","text":"The unequal segregation of organelles has been proposed to be an intrinsic mechanism that contributes to cell fate divergence during asymmetric cell division; however, in vivo evidence is sparse. Using super-resolution microscopy, we analysed the segregation of organelles during the division of the neuroblast QL.p in C. elegans larvae. QL.p divides to generate a daughter that survives, QL.pa, and a daughter that dies, QL.pp. We found that mitochondria segregate unequally by density and morphology and that this is dependent on mitochondrial dynamics. Furthermore, we found that mitochondrial density in QL.pp correlates with the time it takes QL.pp to die. We propose that low mitochondrial density in QL.pp promotes the cell death fate and ensures that QL.pp dies in a highly reproducible and timely manner. Our results provide in vivo evidence that the unequal segregation of mitochondria can contribute to cell fate divergence during asymmetric cell division in a developing animal."}],"article_number":"7174","volume":16,"quality_controlled":"1","department":[{"_id":"CaHe"}],"external_id":{"pmid":["40759648"]},"date_created":"2025-08-17T22:01:35Z","language":[{"iso":"eng"}],"publication_status":"published","day":"04","publisher":"Springer Nature","type":"journal_article","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","date_published":"2025-08-04T00:00:00Z","oa_version":"Published Version","year":"2025","date_updated":"2025-09-01T09:47:29Z","article_processing_charge":"Yes","citation":{"ieee":"I. Segos, J. Van Eeckhoven, S. Berger, N. Mishra, E. J. Lambie, and B. Conradt, “Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","short":"I. Segos, J. Van Eeckhoven, S. Berger, N. Mishra, E.J. Lambie, B. Conradt, Nature Communications 16 (2025).","mla":"Segos, Ioannis, et al. “Unequal Segregation of Mitochondria during Asymmetric Cell Division Contributes to Cell Fate Divergence in Sister Cells in Vivo.” <i>Nature Communications</i>, vol. 16, 7174, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-62484-5\">10.1038/s41467-025-62484-5</a>.","ista":"Segos I, Van Eeckhoven J, Berger S, Mishra N, Lambie EJ, Conradt B. 2025. Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. Nature Communications. 16, 7174.","chicago":"Segos, Ioannis, Jens Van Eeckhoven, Simon Berger, Nikhil Mishra, Eric J. Lambie, and Barbara Conradt. “Unequal Segregation of Mitochondria during Asymmetric Cell Division Contributes to Cell Fate Divergence in Sister Cells in Vivo.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-62484-5\">https://doi.org/10.1038/s41467-025-62484-5</a>.","apa":"Segos, I., Van Eeckhoven, J., Berger, S., Mishra, N., Lambie, E. J., &#38; Conradt, B. (2025). Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-62484-5\">https://doi.org/10.1038/s41467-025-62484-5</a>","ama":"Segos I, Van Eeckhoven J, Berger S, Mishra N, Lambie EJ, Conradt B. Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-62484-5\">10.1038/s41467-025-62484-5</a>"},"PlanS_conform":"1","_id":"20183","title":"Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo","month":"08","OA_place":"publisher","doi":"10.1038/s41467-025-62484-5","article_type":"original","OA_type":"gold","ddc":["570"]},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","date_published":"2025-08-01T00:00:00Z","date_updated":"2025-11-27T14:12:24Z","oa_version":"Published Version","year":"2025","issue":"15","day":"01","publisher":"The Company of Biologists","type":"journal_article","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"OA_type":"hybrid","article_type":"original","doi":"10.1242/jcs.263779","OA_place":"publisher","ddc":["570"],"article_processing_charge":"Yes (via OA deal)","PlanS_conform":"1","citation":{"ieee":"Y. Jikko <i>et al.</i>, “Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration,” <i>Journal of Cell Science</i>, vol. 138, no. 15. The Company of Biologists, 2025.","short":"Y. Jikko, E. Deguchi, K. Matsuda, N. Hino, S. Tsukiji, M. Matsuda, K. Terai, Journal of Cell Science 138 (2025).","mla":"Jikko, Yuya, et al. “Front-Biased Activation of the Ras-Rab5-Rac1 Loop Coordinates Collective Cell Migration.” <i>Journal of Cell Science</i>, vol. 138, no. 15, 263779, The Company of Biologists, 2025, doi:<a href=\"https://doi.org/10.1242/jcs.263779\">10.1242/jcs.263779</a>.","chicago":"Jikko, Yuya, Eriko Deguchi, Kimiya Matsuda, Naoya Hino, Shinya Tsukiji, Michiyuki Matsuda, and Kenta Terai. “Front-Biased Activation of the Ras-Rab5-Rac1 Loop Coordinates Collective Cell Migration.” <i>Journal of Cell Science</i>. The Company of Biologists, 2025. <a href=\"https://doi.org/10.1242/jcs.263779\">https://doi.org/10.1242/jcs.263779</a>.","ista":"Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K. 2025. Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. Journal of Cell Science. 138(15), 263779.","ama":"Jikko Y, Deguchi E, Matsuda K, et al. Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. <i>Journal of Cell Science</i>. 2025;138(15). doi:<a href=\"https://doi.org/10.1242/jcs.263779\">10.1242/jcs.263779</a>","apa":"Jikko, Y., Deguchi, E., Matsuda, K., Hino, N., Tsukiji, S., Matsuda, M., &#38; Terai, K. (2025). Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.263779\">https://doi.org/10.1242/jcs.263779</a>"},"_id":"20188","month":"08","title":"Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration","author":[{"last_name":"Jikko","full_name":"Jikko, Yuya","first_name":"Yuya"},{"first_name":"Eriko","full_name":"Deguchi, Eriko","last_name":"Deguchi"},{"last_name":"Matsuda","full_name":"Matsuda, Kimiya","first_name":"Kimiya"},{"full_name":"Hino, Naoya","last_name":"Hino","first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307"},{"last_name":"Tsukiji","full_name":"Tsukiji, Shinya","first_name":"Shinya"},{"last_name":"Matsuda","full_name":"Matsuda, Michiyuki","first_name":"Michiyuki"},{"first_name":"Kenta","full_name":"Terai, Kenta","last_name":"Terai"}],"publication":"Journal of Cell Science","scopus_import":"1","file_date_updated":"2025-09-01T10:02:24Z","intvolume":"       138","isi":1,"pmid":1,"acknowledgement":"We are grateful to the members of the Matsuda Laboratory for their helpful input, to K. Hirano, T. Uesugi and K. Takakura, who provided technical assistance, and to the Medical Research Support Center of Kyoto University for DNA sequence analysis. This work was supported by the Kyoto University Live Imaging Center. Financial support was provided by Japan Society for the Promotion of Science (JSPS) KAKENHI grants (21H05226 to K.T., 19H00993 and 20H05898 to M.M.), a Japan Science and Technology Agency (JST) CREST grant (JPMJCR1654 to M.M.), and a JST Moonshot Research and Development Program grant (JPMJPS2022 to M.M.). Open Access funding provided by Tokushima University. Deposited in PMC for immediate release.","file":[{"success":1,"relation":"main_file","access_level":"open_access","file_size":12393297,"file_id":"20262","date_created":"2025-09-01T10:02:24Z","file_name":"2025_JourCellScience_Jikko.pdf","creator":"dernst","date_updated":"2025-09-01T10:02:24Z","content_type":"application/pdf","checksum":"29f42619dab5ce251a20c769ed4581c0"}],"publication_identifier":{"issn":[" 0021-9533"],"eissn":["1477-9137"]},"oa":1,"date_created":"2025-08-17T22:01:36Z","external_id":{"isi":["001567723900009"],"pmid":["40667649"]},"department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"publication_status":"published","article_number":"263779","abstract":[{"lang":"eng","text":"Collective cell migration is coordinated by the front-to-rear intercellular propagation of EGFR-Ras-ERK pathway activation. However, the molecular mechanisms integrating front-to-rear information into this intercellular signaling cascade, particularly the determinants of cellular front-side specification, remain elusive. We visualized the activity of EGFR, Ras, Rac1 and Rab5A (hereafter Rab5) by using FRET biosensors and chemogenetic tools. Whereas EGFR activation was uniformly observed within cells, Ras activation was biased to the front side within cells. The polarized Ras activation depended on Merlin and Rac1, which also showed front-biased activation. Furthermore, Rab5, a crucial regulator of cell migration, demonstrated similar front-biased activation and was found to function downstream of Ras while being necessary for Rac1 activation. Thus, the positive feedback loop consisting of Ras, Rab5 and Rac1 is activated primarily at the front of collectively migrating cells. These findings offer new spatio-temporal insight into processing front–rear information during collective cell migration."}],"volume":138,"quality_controlled":"1"},{"publisher":"Elsevier","day":"01","type":"journal_article","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","year":"2025","date_updated":"2025-12-30T10:21:13Z","date_published":"2025-12-01T00:00:00Z","has_accepted_license":"1","citation":{"ieee":"L. Hofmann and C.-P. J. Heisenberg, “Decoding zebrafish oogenesis: From primordial germ cell development to fertilization,” <i>Seminars in Cell and Developmental Biology</i>, vol. 175. Elsevier, 2025.","short":"L. Hofmann, C.-P.J. Heisenberg, Seminars in Cell and Developmental Biology 175 (2025).","mla":"Hofmann, Laura, and Carl-Philipp J. Heisenberg. “Decoding Zebrafish Oogenesis: From Primordial Germ Cell Development to Fertilization.” <i>Seminars in Cell and Developmental Biology</i>, vol. 175, 103650, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">10.1016/j.semcdb.2025.103650</a>.","chicago":"Hofmann, Laura, and Carl-Philipp J Heisenberg. “Decoding Zebrafish Oogenesis: From Primordial Germ Cell Development to Fertilization.” <i>Seminars in Cell and Developmental Biology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">https://doi.org/10.1016/j.semcdb.2025.103650</a>.","ista":"Hofmann L, Heisenberg C-PJ. 2025. Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. Seminars in Cell and Developmental Biology. 175, 103650.","ama":"Hofmann L, Heisenberg C-PJ. Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. <i>Seminars in Cell and Developmental Biology</i>. 2025;175. doi:<a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">10.1016/j.semcdb.2025.103650</a>","apa":"Hofmann, L., &#38; Heisenberg, C.-P. J. (2025). Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. <i>Seminars in Cell and Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">https://doi.org/10.1016/j.semcdb.2025.103650</a>"},"PlanS_conform":"1","article_processing_charge":"Yes (via OA deal)","title":"Decoding zebrafish oogenesis: From primordial germ cell development to fertilization","month":"12","_id":"20349","OA_place":"publisher","doi":"10.1016/j.semcdb.2025.103650","OA_type":"hybrid","article_type":"review","ddc":["570"],"publication_identifier":{"issn":["1084-9521"],"eissn":["1096-3634"]},"oa":1,"file":[{"relation":"main_file","access_level":"open_access","success":1,"date_updated":"2025-12-30T10:21:00Z","checksum":"80ea6cbb004853bb1e87db3422a74aca","content_type":"application/pdf","file_size":2778561,"file_id":"20914","date_created":"2025-12-30T10:21:00Z","file_name":"2025_SemCellDevBiology_Hofmann.pdf","creator":"dernst"}],"acknowledgement":"We thank Carolina Camelo for making schematics for this review.","pmid":1,"isi":1,"intvolume":"       175","scopus_import":"1","author":[{"first_name":"Laura","id":"b88d43f2-dc74-11ea-a0a7-e41b7912e031","full_name":"Hofmann, Laura","last_name":"Hofmann"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publication":"Seminars in Cell and Developmental Biology","file_date_updated":"2025-12-30T10:21:00Z","abstract":[{"lang":"eng","text":"Oogenesis – the formation and development of an oocyte – is fundamental to reproduction and embryonic development. Due to its accessibility to genetic manipulations and the ability to culture and experimentally manipulate oocytes ex vivo, zebrafish has emerged as a powerful vertebrate model system for studying oogenesis. In this review, we provide a comprehensive overview of zebrafish oogenesis, from early germ cell formation to oocyte maturation and fertilization. We discuss recent advances in uncovering the molecular and cellular mechanisms driving this complex process and highlight key knowledge gaps that remain to be addressed."}],"article_number":"103650","quality_controlled":"1","volume":175,"language":[{"iso":"eng"}],"corr_author":"1","external_id":{"pmid":["40913907"],"isi":["001567260100001"]},"department":[{"_id":"CaHe"}],"date_created":"2025-09-14T22:01:32Z","publication_status":"published"},{"_id":"19404","month":"03","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","article_processing_charge":"Yes","citation":{"ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>.","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>"},"ddc":["570"],"OA_type":"gold","article_type":"original","OA_place":"publisher","doi":"10.1016/j.celrep.2025.115387","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"issue":"3","type":"journal_article","day":"25","publisher":"Elsevier","date_published":"2025-03-25T00:00:00Z","has_accepted_license":"1","project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"},{"name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","grant_number":"ALTF 1159-2018","_id":"269CD5C4-B435-11E9-9278-68D0E5697425"}],"date_updated":"2025-10-22T07:00:04Z","oa_version":"Published Version","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":44,"quality_controlled":"1","article_number":"115387","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","abstract":[{"lang":"eng","text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"publication_status":"published","date_created":"2025-03-16T23:01:24Z","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"external_id":{"isi":["001443652700001"],"pmid":["40057955"]},"corr_author":"1","language":[{"iso":"eng"}],"pmid":1,"acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","file":[{"file_size":9067797,"file_id":"19413","date_created":"2025-03-17T10:26:54Z","creator":"dernst","file_name":"2025_CellReports_Tavano.pdf","date_updated":"2025-03-17T10:26:54Z","checksum":"57e05dd1598c807af0afdb32cec039d3","content_type":"application/pdf","success":1,"relation":"main_file","access_level":"open_access"}],"DOAJ_listed":"1","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"oa":1,"author":[{"full_name":"Tavano, Ste","last_name":"Tavano","first_name":"Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9970-7804"},{"last_name":"Brückner","full_name":"Brückner, David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David","orcid":"0000-0001-7205-2975"},{"orcid":"0000-0003-1671-393X","last_name":"Tasciyan","full_name":"Tasciyan, Saren","first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Tong, Xin","last_name":"Tong","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin"},{"full_name":"Kardos, Roland","last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schauer, Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","first_name":"Alexandra","orcid":"0000-0001-7659-9142"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"scopus_import":"1","publication":"Cell Reports","file_date_updated":"2025-03-17T10:26:54Z","intvolume":"        44","isi":1},{"supervisor":[{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561"}],"abstract":[{"text":"Epithelial spreading plays a pivotal role in the development of organisms especially those\r\nsuch as zebrafish which require the epithelial enveloping layer (EVL) to spread to cover the\r\nsubstantial yolk surface during gastrulation. Epiboly requires the transition of the epithelium\r\nwith cuboidal cells to form a thin, flat squamous epithelial sheet. During this transition, the\r\ncells show tissue-scale mechanosensation with mechanisms such as direct mechanical control\r\nover the axis of cell division.\r\nCytoskeletal intermediate filaments play a crucial role in vertebrate cells, not only facilitating\r\nmechanical stability but also helping facilitate the mechanosensitive response of the cell.\r\nMechanosenstivity displayed by intermediate filaments is due not just to their interesting\r\nphysical properties but also to their interactions with other cytoskeletal elements such as actin\r\nand microtubules. Keratin is the predominant intermediate filament expressed in the EVL.\r\nIt expresses concomitantly with the gastrulation movements of the developing embryo. Our\r\nwork focuses on understanding the role and dynamics of the keratin cytoskeletal network in\r\nmodulating the physical aspects of EVL spreading. We demonstrated with the combination of\r\nphysical characterisation and manipulations of the EVL, utilising a variety of biophysical tools\r\nand microscopy, the mechanistic role of keratin in tissue spreading.\r\nGenerating novel genetic morphants and mutants, we probe the effect that the loss of the\r\nkeratin network has on the physiology of the epithelium and the developing embryo. We\r\nshow that the changing organisation of the keratin network is important for changing EVL\r\nphysical properties as the stress imposed on the EVL increases during epiboly. By modelling\r\nthe epithelium, we study how the mechanical heterogeneity in an epithelium can feed back into\r\na mechanical loop to the maturation of the keratin network and hence affect the mechanics\r\nof the epithelium. However, unlike what would be predicted by the effect of intermediate\r\nfilaments in acting as a security belt and increasing the resistance of the epithelium, we observe\r\nthat loss of keratin leads to a delay in the EVL movement. Using both local aspirations of the\r\nYSL and EVL ablations, we demonstrate the mechanistic facilitation of actin mechanosensation\r\nin a keratin-dependent manner.\r\nFurthermore, using chemical inhibitors of microtubule polymerisation, we provide insight into\r\nthe mechanisms underlying the organisation and distribution of keratin. Interestingly, the\r\nphenotype observed upon this loss of microtubules shows that keratins interact with the nucleus\r\nthrough microtubular interactions. Together with these diverse observations, we describe\r\nthe mechanosensory feedback between resilience and that is critical for uniform and robust\r\nspreading of the epithelium.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"degree_awarded":"PhD","publication_status":"published","date_created":"2025-10-10T14:58:30Z","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"language":[{"iso":"eng"}],"corr_author":"1","file":[{"file_size":6846189,"file_name":"Thesis_PDFA_.pdf","creator":"snaik","date_created":"2025-10-28T13:10:08Z","file_id":"20567","date_updated":"2025-10-28T13:10:08Z","checksum":"2892f04d4a5c18677871c3e06ac1244a","content_type":"application/pdf","success":1,"access_level":"open_access","relation":"main_file"},{"content_type":"application/zip","checksum":"15934d4465cd0e9b7c32678da9a33a2f","date_updated":"2025-10-28T13:10:26Z","date_created":"2025-10-28T13:10:26Z","file_id":"20568","creator":"snaik","file_name":"Thesis.zip","file_size":8839300,"relation":"source_file","access_level":"open_access"}],"acknowledgement":"I would also like to thank the LSF and Cryo facility at ISTA, which have been helpful in my\r\nexperiments. I would also like to acknowledge FWF, grant DOI 10.55776/PAT5044023 and JKU Nanocell grant DOI \r\n10.55776/W1250 for providing funding for my PhD research. EMBO and FWF for providing funding for travel grants to attend conferences.","publication_identifier":{"isbn":["978-3-99078-069-5"],"issn":["2663-337X"]},"oa":1,"file_date_updated":"2025-10-28T13:10:26Z","author":[{"orcid":"0000-0001-8421-5508","first_name":"Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","full_name":"Naik, Suyash","last_name":"Naik"}],"_id":"20441","month":"10","title":"Keratins act as global coordinators of tissue spreading through mechanosensitive feedback","article_processing_charge":"No","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"20465"}]},"citation":{"chicago":"Naik, Suyash. “Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>.","ista":"Naik S. 2025. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. Institute of Science and Technology Austria.","ama":"Naik S. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>","apa":"Naik, S. (2025). <i>Keratins act as global coordinators of tissue spreading through mechanosensitive feedback</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>","ieee":"S. Naik, “Keratins act as global coordinators of tissue spreading through mechanosensitive feedback,” Institute of Science and Technology Austria, 2025.","mla":"Naik, Suyash. <i>Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>.","short":"S. Naik, Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback, Institute of Science and Technology Austria, 2025."},"ddc":["596","597","532"],"alternative_title":["ISTA Thesis"],"doi":"10.15479/AT-ISTA-20441","OA_place":"publisher","status":"public","tmp":{"short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png"},"publisher":"Institute of Science and Technology Austria","type":"dissertation","day":"12","project":[{"grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46","name":"Keratins in epithelial tissue spreading"},{"grant_number":"W 1250-B20","_id":"25AA5F24-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF"}],"has_accepted_license":"1","date_published":"2025-10-12T00:00:00Z","date_updated":"2026-04-07T11:58:57Z","oa_version":"Published Version","year":"2025","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"105"},{"status":"public","oa":1,"tmp":{"name":"Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)","image":"/image/cc_by_nd.png","short":"CC BY-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nd/4.0/legalcode"},"publisher":"Cold Spring Harbor Laboratory","day":"17","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.14.638262"}],"type":"preprint","date_published":"2025-02-17T00:00:00Z","year":"2025","oa_version":"Preprint","date_updated":"2026-04-07T11:58:57Z","author":[{"last_name":"Naik","full_name":"Naik, Suyash","first_name":"Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8421-5508"},{"full_name":"Keta, Yann-Edwin","last_name":"Keta","first_name":"Yann-Edwin"},{"id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Henkes","full_name":"Henkes, Silke","first_name":"Silke"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publication":"bioRxiv","_id":"20465","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","month":"02","license":"https://creativecommons.org/licenses/by-nd/4.0/","article_processing_charge":"No","abstract":[{"lang":"eng","text":"For tissues to spread, they must be deformable while maintaining their structural integrity. How these opposing requirements are balanced within spreading tissues is not yet well understood. Here, we show that keratin intermediate filaments function in epithelial spreading by adapting tissue mechanical resilience to the stresses arising in the tissue during the spreading process. By analysing the expansion of the enveloping cell layer (EVL) over the large yolk cell in early zebrafish embryos in vivo, we found that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, which is essential for preventing tissue rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the force-generating processes pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production in the yolk cell and the mechanical resilience of the EVL against stresses generated by these pulling forces, thereby ensuring uniform and robust tissue spreading."}],"citation":{"ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, n.d. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>.","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. Cold Spring Harbor Laboratory. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory.","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, BioRxiv (n.d.).","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>."},"related_material":{"record":[{"id":"20441","relation":"dissertation_contains","status":"public"}]},"publication_status":"draft","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"date_created":"2025-10-14T07:25:27Z","doi":"10.1101/2025.02.14.638262","OA_place":"repository","corr_author":"1","language":[{"iso":"eng"}]},{"page":"R1230-R1232","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-09T11:51:15Z","year":"2024","oa_version":"None","date_published":"2024-12-16T00:00:00Z","issue":"24","day":"16","publisher":"Elsevier","type":"journal_article","status":"public","OA_type":"closed access","article_type":"letter_note","doi":"10.1016/j.cub.2024.10.065","citation":{"ieee":"N. Hino, C. Santos Fernandes Lasbarrères Camelo, and C.-P. J. Heisenberg, “Development: Turing mechanics,” <i>Current Biology</i>, vol. 34, no. 24. Elsevier, pp. R1230–R1232, 2024.","mla":"Hino, Naoya, et al. “Development: Turing Mechanics.” <i>Current Biology</i>, vol. 34, no. 24, Elsevier, 2024, pp. R1230–32, doi:<a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">10.1016/j.cub.2024.10.065</a>.","short":"N. Hino, C. Santos Fernandes Lasbarrères Camelo, C.-P.J. Heisenberg, Current Biology 34 (2024) R1230–R1232.","ista":"Hino N, Santos Fernandes Lasbarrères Camelo C, Heisenberg C-PJ. 2024. Development: Turing mechanics. Current Biology. 34(24), R1230–R1232.","chicago":"Hino, Naoya, Carolina Santos Fernandes Lasbarrères Camelo, and Carl-Philipp J Heisenberg. “Development: Turing Mechanics.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">https://doi.org/10.1016/j.cub.2024.10.065</a>.","apa":"Hino, N., Santos Fernandes Lasbarrères Camelo, C., &#38; Heisenberg, C.-P. J. (2024). Development: Turing mechanics. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">https://doi.org/10.1016/j.cub.2024.10.065</a>","ama":"Hino N, Santos Fernandes Lasbarrères Camelo C, Heisenberg C-PJ. Development: Turing mechanics. <i>Current Biology</i>. 2024;34(24):R1230-R1232. doi:<a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">10.1016/j.cub.2024.10.065</a>"},"article_processing_charge":"No","month":"12","title":"Development: Turing mechanics","_id":"18651","intvolume":"        34","isi":1,"scopus_import":"1","author":[{"first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","full_name":"Hino, Naoya","last_name":"Hino"},{"id":"6347dca5-074c-11ed-af92-a80f860d9d5b","first_name":"Carolina","full_name":"Santos Fernandes Lasbarrères Camelo, Carolina","last_name":"Santos Fernandes Lasbarrères Camelo"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"publication":"Current Biology","publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"pmid":1,"corr_author":"1","language":[{"iso":"eng"}],"date_created":"2024-12-15T23:01:49Z","external_id":{"isi":["001392077000001"],"pmid":["39689690"]},"department":[{"_id":"CaHe"}],"publication_status":"published","abstract":[{"text":"Embryo axis formation begins with the localized expression of biochemical signals, which organize cell movements and determine cell fate. A quail study finds that tissue contraction and resulting long-range changes in tissue tension restrict the area where these biochemical signals are expressed.","lang":"eng"}],"quality_controlled":"1","volume":34},{"doi":"10.7554/elife.80803","OA_place":"publisher","OA_type":"gold","article_type":"original","ddc":["570"],"article_processing_charge":"Yes","citation":{"ista":"Knabl P, Schauer A, Pomreinke AP, Zimmermann B, Rogers KW, Čapek D, Müller P, Genikhovich G. 2024. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. eLife. 13.","chicago":"Knabl, Paul, Alexandra Schauer, Autumn P Pomreinke, Bob Zimmermann, Katherine W Rogers, Daniel Čapek, Patrick Müller, and Grigory Genikhovich. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>.","apa":"Knabl, P., Schauer, A., Pomreinke, A. P., Zimmermann, B., Rogers, K. W., Čapek, D., … Genikhovich, G. (2024). Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>","ama":"Knabl P, Schauer A, Pomreinke AP, et al. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>eLife</i>. 2024;13. doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>","ieee":"P. Knabl <i>et al.</i>, “Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator,” <i>eLife</i>, vol. 13. eLife Sciences Publications, 2024.","short":"P. Knabl, A. Schauer, A.P. Pomreinke, B. Zimmermann, K.W. Rogers, D. Čapek, P. Müller, G. Genikhovich, ELife 13 (2024).","mla":"Knabl, Paul, et al. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>, vol. 13, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>."},"_id":"18940","title":"Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator","month":"02","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2024-02-07T00:00:00Z","has_accepted_license":"1","oa_version":"Published Version","year":"2024","date_updated":"2025-01-29T08:56:21Z","type":"journal_article","day":"07","publisher":"eLife Sciences Publications","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"department":[{"_id":"CaHe"}],"date_created":"2025-01-29T08:48:34Z","language":[{"iso":"eng"}],"publication_status":"published","abstract":[{"lang":"eng","text":"BMP signaling has a conserved function in patterning the dorsal-ventral body axis in Bilateria and the directive axis in anthozoan cnidarians. So far, cnidarian studies have focused on the role of different BMP signaling network components in regulating pSMAD1/5 gradient formation. Much less is known about the target genes downstream of BMP signaling. To address this, we generated a genome-wide list of direct pSMAD1/5 target genes in the anthozoan <jats:italic>Nematostella vectensis</jats:italic>, several of which were conserved in <jats:italic>Drosophila</jats:italic> and <jats:italic>Xenopus</jats:italic>. Our ChIP-seq analysis revealed that many of the regulatory molecules with documented bilaterally symmetric expression in <jats:italic>Nematostella</jats:italic> are directly controlled by BMP signaling. We identified several so far uncharacterized BMP-dependent transcription factors and signaling molecules, whose bilaterally symmetric expression may be indicative of their involvement in secondary axis patterning. One of these molecules is <jats:italic>zswim4-6</jats:italic>, which encodes a novel nuclear protein that can modulate the pSMAD1/5 gradient and potentially promote BMP-dependent gene repression."}],"volume":13,"quality_controlled":"1","file_date_updated":"2025-01-29T08:50:18Z","publication":"eLife","author":[{"last_name":"Knabl","full_name":"Knabl, Paul","first_name":"Paul"},{"first_name":"Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer","full_name":"Schauer, Alexandra","orcid":"0000-0001-7659-9142"},{"last_name":"Pomreinke","full_name":"Pomreinke, Autumn P","first_name":"Autumn P"},{"first_name":"Bob","full_name":"Zimmermann, Bob","last_name":"Zimmermann"},{"first_name":"Katherine W","last_name":"Rogers","full_name":"Rogers, Katherine W"},{"last_name":"Čapek","full_name":"Čapek, Daniel","first_name":"Daniel"},{"first_name":"Patrick","full_name":"Müller, Patrick","last_name":"Müller"},{"first_name":"Grigory","last_name":"Genikhovich","full_name":"Genikhovich, Grigory"}],"scopus_import":"1","intvolume":"        13","file":[{"checksum":"24548a184215d3f4547bba535ccfd7b1","content_type":"application/pdf","date_updated":"2025-01-29T08:50:18Z","creator":"dernst","file_name":"2024_eLife_Knabl.pdf","file_id":"18941","date_created":"2025-01-29T08:50:18Z","file_size":11855972,"access_level":"open_access","relation":"main_file","success":1}],"DOAJ_listed":"1","acknowledgement":"This work was funded by the Austrian Science Foundation (FWF) grants P26962-B21 and P32705-B to GG and by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 637840 [QUANTPATTERN] and 863952 [ACE-OF-SPACE]) to PM. We thank Michaela Schwaiger, Taras Kreslavsky, Hiromi Tagoh, and Patricio Ferrer Murguia for their help with the ChIP protocol, Matthias Richter and Christian Hofer for their assistance with in situ analyses, Emilio Gonzalez Morales for making the measurements for Figure 6—figure supplement 3, Catrin Weiler for the assistance in cloning zebrafish zswim5, David Mörsdorf for critically reading the manuscript and help with data visualization, and the Core Facility for Cell Imaging and Ultrastructure Research of the University of Vienna for access to the confocal microscope.","oa":1,"publication_identifier":{"issn":["2050-084X"]}},{"file_date_updated":"2024-01-16T10:53:31Z","scopus_import":"1","publication":"Current Biology","author":[{"id":"49DA7910-F248-11E8-B48F-1D18A9856A87","first_name":"Feyza N","full_name":"Arslan, Feyza N","last_name":"Arslan","orcid":"0000-0001-5809-9566"},{"full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"last_name":"Loose","full_name":"Loose, Martin","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7309-9724"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"ec_funded":1,"isi":1,"intvolume":"        34","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","file":[{"creator":"dernst","file_name":"2024_CurrentBiology_Arslan.pdf","file_id":"14813","date_created":"2024-01-16T10:53:31Z","file_size":5183861,"checksum":"51220b76d72a614208f84bdbfbaf9b72","content_type":"application/pdf","date_updated":"2024-01-16T10:53:31Z","success":1,"access_level":"open_access","relation":"main_file"}],"pmid":1,"publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"oa":1,"publication_status":"published","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"external_id":{"pmid":["38134934"],"isi":["001154500400001"]},"date_created":"2024-01-14T23:00:56Z","language":[{"iso":"eng"}],"corr_author":"1","volume":34,"quality_controlled":"1","abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"has_accepted_license":"1","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"date_published":"2024-01-08T00:00:00Z","oa_version":"Published Version","year":"2024","date_updated":"2025-09-04T11:39:10Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"171-182.e8","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","day":"08","publisher":"Elsevier","issue":"1","ddc":["570"],"doi":"10.1016/j.cub.2023.11.067","article_type":"original","_id":"14795","title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","month":"01","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>","chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>.","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8."}},{"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}],"quality_controlled":"1","volume":20,"language":[{"iso":"eng"}],"corr_author":"1","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"external_id":{"pmid":["38370025"],"isi":["001138880800005"]},"date_created":"2024-01-21T23:00:57Z","publication_status":"published","oa":1,"publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","file":[{"success":1,"access_level":"open_access","relation":"main_file","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","creator":"dernst","file_id":"17267","date_created":"2024-07-16T12:12:43Z","file_size":9897883,"content_type":"application/pdf","checksum":"7891ebe7c900ae47469ab127031dd1ec","date_updated":"2024-07-16T12:12:43Z"}],"pmid":1,"isi":1,"intvolume":"        20","author":[{"orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Shinde, Rushikesh","last_name":"Shinde","first_name":"Rushikesh"},{"id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","first_name":"Madison","last_name":"Bolger-Munro","full_name":"Bolger-Munro, Madison","orcid":"0000-0002-8176-4824"},{"full_name":"Peruzzo, Matilda","last_name":"Peruzzo","first_name":"Matilda","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3415-4628"},{"last_name":"Szep","full_name":"Szep, Gregory","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory"},{"first_name":"Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","full_name":"Steccari, Irene","last_name":"Steccari"},{"full_name":"Labrousse Arias, David","last_name":"Labrousse Arias","first_name":"David","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425"},{"orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","full_name":"Zheden, Vanessa","last_name":"Zheden"},{"orcid":"0000-0001-5145-4609","last_name":"Merrin","full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Andrew","last_name":"Callan-Jones","full_name":"Callan-Jones, Andrew"},{"first_name":"Raphaël","last_name":"Voituriez","full_name":"Voituriez, Raphaël"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"publication":"Nature Physics","scopus_import":"1","file_date_updated":"2024-07-16T12:12:43Z","citation":{"ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>"},"related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","description":"News on ISTA Website"}]},"article_processing_charge":"Yes (in subscription journal)","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","month":"02","_id":"14846","doi":"10.1038/s41567-023-02302-1","article_type":"original","ddc":["530"],"publisher":"Springer Nature","day":"01","type":"journal_article","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","page":"310-321","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2024","oa_version":"Published Version","date_updated":"2025-09-04T11:48:28Z","has_accepted_license":"1","project":[{"call_identifier":"FWF","name":"Control of embryonic cleavage pattern","_id":"2646861A-B435-11E9-9278-68D0E5697425","grant_number":"I03601"}],"date_published":"2024-02-01T00:00:00Z"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"1-18","has_accepted_license":"1","date_published":"2024-02-01T00:00:00Z","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239"}],"date_updated":"2025-09-04T12:10:40Z","oa_version":"Published Version","year":"2024","issue":"4","type":"journal_article","day":"01","publisher":"The Company of Biologists","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","doi":"10.1242/dev.202316","ddc":["570"],"article_processing_charge":"Yes (via OA deal)","related_material":{"record":[{"relation":"research_data","status":"public","id":"14926"}]},"citation":{"ieee":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>, vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.","short":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development 151 (2024) 1–18.","mla":"Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, The Company of Biologists, 2024, pp. 1–18, doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>.","ista":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4), 1–18.","chicago":"Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>.","apa":"Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J. (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>","ama":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18. doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>"},"_id":"15048","month":"02","title":"Robust axis elongation by Nodal-dependent restriction of BMP signaling","file_date_updated":"2024-03-04T07:24:43Z","scopus_import":"1","author":[{"full_name":"Schauer, Alexandra","last_name":"Schauer","first_name":"Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7659-9142"},{"last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija","first_name":"Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"publication":"Development","intvolume":"       151","isi":1,"ec_funded":1,"pmid":1,"acknowledgement":"We thank Patrick Müller for sharing the chordintt250 mutant zebrafish line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro and Katherine Rogers and members of the Heisenberg lab for discussions, technical advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo for discussions. We thank the Imaging and Optics Facility as well as the Life Science facility at IST Austria for support with microscopy and fish maintenance.\r\nThis work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573 to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience and Technology Austria. ","file":[{"success":1,"relation":"main_file","access_level":"open_access","file_size":14839986,"date_created":"2024-03-04T07:24:43Z","file_id":"15050","file_name":"2024_Development_Schauer.pdf","creator":"dernst","date_updated":"2024-03-04T07:24:43Z","checksum":"6961ea10012bf0d266681f9628bb8f13","content_type":"application/pdf"}],"oa":1,"publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"date_created":"2024-03-03T23:00:50Z","department":[{"_id":"CaHe"},{"_id":"Bio"}],"external_id":{"isi":["001170580200001"],"pmid":["38372390"]},"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published","abstract":[{"lang":"eng","text":"Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play key roles in germ layer specification and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants to study the coordination of Nodal and BMP signaling for embryo patterning and morphogenesis. We show that Nodal signaling triggers explant elongation by inducing mesendodermal progenitors but also suppressing BMP signaling activity at the site of mesendoderm induction. Consistent with this, ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm intercalations, key processes during explant elongation. Translating these ex vivo observations to the intact embryo showed that, similar to explants, Nodal signaling suppresses the effect of BMP signaling on cell intercalations in the dorsal domain, thus allowing robust embryonic axis elongation. These findings suggest a dual function of Nodal signaling in embryonic axis elongation by both inducing mesendoderm and suppressing BMP effects in the dorsal portion of the mesendoderm."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":151,"quality_controlled":"1"},{"department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"external_id":{"pmid":["38579717"],"isi":["001301584600001"]},"date_created":"2024-04-08T12:07:57Z","language":[{"iso":"eng"}],"corr_author":"1","publication_status":"published","abstract":[{"lang":"eng","text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":59,"quality_controlled":"1","scopus_import":"1","author":[{"first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926"},{"orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","full_name":"Montesinos López, Juan C","last_name":"Montesinos López"},{"first_name":"N","full_name":"Trozzi, N","last_name":"Trozzi"},{"full_name":"Spona, Leonhard","last_name":"Spona","id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard"},{"full_name":"Yoshida, Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra","full_name":"Marhavá, Petra","last_name":"Marhavá"},{"orcid":"0000-0002-5223-3346","last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Y","last_name":"Dagdas","full_name":"Dagdas, Y"},{"last_name":"Majda","full_name":"Majda, M","first_name":"M"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"publication":"Developmental Cell","file_date_updated":"2024-08-20T11:22:16Z","ec_funded":1,"isi":1,"intvolume":"        59","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","file":[{"checksum":"22b374fb50a40d380b7686c84258d271","content_type":"application/pdf","date_updated":"2024-08-20T11:22:16Z","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","creator":"dernst","file_id":"17452","date_created":"2024-08-20T11:22:16Z","file_size":5195262,"access_level":"open_access","relation":"main_file","success":1}],"pmid":1,"oa":1,"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"doi":"10.1016/j.devcel.2024.03.009","article_type":"original","ddc":["570"],"article_processing_charge":"Yes (via OA deal)","citation":{"ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>"},"related_material":{"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/","relation":"press_release"}]},"_id":"15301","title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","month":"05","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"1333-1344.e4","date_published":"2024-05-20T00:00:00Z","has_accepted_license":"1","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988"}],"year":"2024","oa_version":"Published Version","date_updated":"2025-09-04T13:32:08Z","day":"20","type":"journal_article","publisher":"Elsevier","issue":"10","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"intvolume":"         6","isi":1,"file_date_updated":"2023-08-14T07:17:36Z","publication":"Communications Biology","author":[{"first_name":"Elod","full_name":"Méhes, Elod","last_name":"Méhes"},{"full_name":"Mones, Enys","last_name":"Mones","first_name":"Enys"},{"last_name":"Varga","full_name":"Varga, Máté","first_name":"Máté"},{"last_name":"Zsigmond","full_name":"Zsigmond, Áron","first_name":"Áron"},{"last_name":"Biri-Kovács","full_name":"Biri-Kovács, Beáta","first_name":"Beáta"},{"last_name":"Nyitray","full_name":"Nyitray, László","first_name":"László"},{"full_name":"Barone, Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","orcid":"0000-0003-2676-3367"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","last_name":"Krens","full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"full_name":"Vicsek, Tamás","last_name":"Vicsek","first_name":"Tamás"}],"scopus_import":"1","oa":1,"publication_identifier":{"eissn":["2399-3642"]},"pmid":1,"file":[{"file_size":10181997,"creator":"dernst","file_name":"2023_CommBiology_Mehes.pdf","file_id":"14045","date_created":"2023-08-14T07:17:36Z","date_updated":"2023-08-14T07:17:36Z","content_type":"application/pdf","checksum":"1f9324f736bdbb76426b07736651c4cd","success":1,"access_level":"open_access","relation":"main_file"}],"acknowledgement":"We thank Marton Gulyas (ELTE Eötvös University) for development of videomicroscopy experiment manager and image analysis software. Authors are grateful to Gabor Forgacs (University of Missouri) for critical reading of earlier versions of this manuscript as well as to Zsuzsa Akos and Andras Czirok (ELTE Eötvös University) for fruitful discussions. This work was supported by EU FP7, ERC COLLMOT Project No 227878 to TV, the National Research Development and Innovation Fund of Hungary, K119359 and also Project No 2018-1.2.1-NKP-2018-00005 to LN. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 955576. MV was supported by the Ja´nos Bolyai Fellowship of the Hungarian Academy of Sciences.\r\nOpen access funding provided by Eötvös Loránd University.","language":[{"iso":"eng"}],"date_created":"2023-08-13T22:01:13Z","external_id":{"pmid":["37542157"],"isi":["001042544100001"]},"department":[{"_id":"CaHe"},{"_id":"Bio"}],"publication_status":"published","article_number":"817","abstract":[{"text":"Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated by cell types through controlling cell-cell contact formation by regulating adhesion and actomyosin contractility-based cellular cortical tensions. We use vertebrate tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional heterotypic segregation and developed a quantitative analysis of their dynamics based on 3D time-lapse microscopy. We show that general inhibition of actomyosin contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific inhibition of non-muscle myosin2 activity by overexpression of myosin assembly inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction during aggregation and inverted geometry observed during segregation. The same is observed when we express a constitutively active Rho kinase isoform to ubiquitously keep actomyosin contractility high at cell-cell and cell-medium interfaces and thus overriding the interface-specific regulation of cortical tensions. Tissue surface tension regulation can become an effective tool in tissue engineering.","lang":"eng"}],"quality_controlled":"1","volume":6,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2023-12-13T12:07:33Z","year":"2023","oa_version":"Published Version","has_accepted_license":"1","date_published":"2023-08-04T00:00:00Z","publisher":"Springer Nature","day":"04","type":"journal_article","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","article_type":"original","doi":"10.1038/s42003-023-05181-7","ddc":["570"],"citation":{"apa":"Méhes, E., Mones, E., Varga, M., Zsigmond, Á., Biri-Kovács, B., Nyitray, L., … Vicsek, T. (2023). 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>","ama":"Méhes E, Mones E, Varga M, et al. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. 2023;6. doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>","ista":"Méhes E, Mones E, Varga M, Zsigmond Á, Biri-Kovács B, Nyitray L, Barone V, Krens G, Heisenberg C-PJ, Vicsek T. 2023. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. 6, 817.","chicago":"Méhes, Elod, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács, László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp J Heisenberg, and Tamás Vicsek. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>.","short":"E. Méhes, E. Mones, M. Varga, Á. Zsigmond, B. Biri-Kovács, L. Nyitray, V. Barone, G. Krens, C.-P.J. Heisenberg, T. Vicsek, Communications Biology 6 (2023).","mla":"Méhes, Elod, et al. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>, vol. 6, 817, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>.","ieee":"E. Méhes <i>et al.</i>, “3D cell segregation geometry and dynamics are governed by tissue surface tension regulation,” <i>Communications Biology</i>, vol. 6. Springer Nature, 2023."},"article_processing_charge":"Yes","month":"08","title":"3D cell segregation geometry and dynamics are governed by tissue surface tension regulation","_id":"14041"},{"ddc":["570"],"article_type":"review","doi":"10.1016/j.ceb.2023.102217","month":"10","title":"Stretching the limits of extracellular signal-related kinase (ERK) signaling — Cell mechanosensing to ERK activation","_id":"14080","citation":{"ieee":"T. Hirashima, N. Hino, K. Aoki, and M. Matsuda, “Stretching the limits of extracellular signal-related kinase (ERK) signaling — Cell mechanosensing to ERK activation,” <i>Current Opinion in Cell Biology</i>, vol. 84, no. 10. Elsevier, 2023.","mla":"Hirashima, Tsuyoshi, et al. “Stretching the Limits of Extracellular Signal-Related Kinase (ERK) Signaling — Cell Mechanosensing to ERK Activation.” <i>Current Opinion in Cell Biology</i>, vol. 84, no. 10, 102217, Elsevier, 2023, doi:<a href=\"https://doi.org/10.1016/j.ceb.2023.102217\">10.1016/j.ceb.2023.102217</a>.","short":"T. Hirashima, N. Hino, K. Aoki, M. Matsuda, Current Opinion in Cell Biology 84 (2023).","chicago":"Hirashima, Tsuyoshi, Naoya Hino, Kazuhiro Aoki, and Michiyuki Matsuda. “Stretching the Limits of Extracellular Signal-Related Kinase (ERK) Signaling — Cell Mechanosensing to ERK Activation.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.ceb.2023.102217\">https://doi.org/10.1016/j.ceb.2023.102217</a>.","ista":"Hirashima T, Hino N, Aoki K, Matsuda M. 2023. Stretching the limits of extracellular signal-related kinase (ERK) signaling — Cell mechanosensing to ERK activation. Current Opinion in Cell Biology. 84(10), 102217.","ama":"Hirashima T, Hino N, Aoki K, Matsuda M. Stretching the limits of extracellular signal-related kinase (ERK) signaling — Cell mechanosensing to ERK activation. <i>Current Opinion in Cell Biology</i>. 2023;84(10). doi:<a href=\"https://doi.org/10.1016/j.ceb.2023.102217\">10.1016/j.ceb.2023.102217</a>","apa":"Hirashima, T., Hino, N., Aoki, K., &#38; Matsuda, M. (2023). Stretching the limits of extracellular signal-related kinase (ERK) signaling — Cell mechanosensing to ERK activation. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2023.102217\">https://doi.org/10.1016/j.ceb.2023.102217</a>"},"article_processing_charge":"Yes (in subscription journal)","date_updated":"2024-01-30T12:52:42Z","oa_version":"Published Version","year":"2023","has_accepted_license":"1","date_published":"2023-10-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","issue":"10","day":"01","publisher":"Elsevier","type":"journal_article","publication_status":"published","language":[{"iso":"eng"}],"date_created":"2023-08-20T22:01:12Z","department":[{"_id":"CaHe"}],"external_id":{"isi":["001054692200001"],"pmid":["37574635"]},"quality_controlled":"1","volume":84,"article_number":"102217","abstract":[{"lang":"eng","text":"Extracellular signal-regulated kinase (ERK) has been recognized as a critical regulator in various physiological and pathological processes. Extensive research has elucidated the signaling mechanisms governing ERK activation via biochemical regulations with upstream molecules, particularly receptor tyrosine kinases (RTKs). However, recent advances have highlighted the role of mechanical forces in activating the RTK–ERK signaling pathways, thereby opening new avenues of research into mechanochemical interplay in multicellular tissues. Here, we review the force-induced ERK activation in cells and propose possible mechanosensing mechanisms underlying the mechanoresponsive ERK activation. We conclude that mechanical forces are not merely passive factors shaping cells and tissues but also active regulators of cellular signaling pathways controlling collective cell behaviors."}],"isi":1,"intvolume":"        84","author":[{"last_name":"Hirashima","full_name":"Hirashima, Tsuyoshi","first_name":"Tsuyoshi"},{"last_name":"Hino","full_name":"Hino, Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","first_name":"Naoya"},{"full_name":"Aoki, Kazuhiro","last_name":"Aoki","first_name":"Kazuhiro"},{"first_name":"Michiyuki","last_name":"Matsuda","full_name":"Matsuda, Michiyuki"}],"file_date_updated":"2024-01-30T12:52:12Z","scopus_import":"1","publication":"Current Opinion in Cell Biology","oa":1,"publication_identifier":{"eissn":["1879-0410"],"issn":["0955-0674"]},"pmid":1,"acknowledgement":"TH was supported by JSPS KAKENHI Grant (no. 21H05290) and the Ministry of Education under the Research Centres of Excellence programme through the Mechanobiology Institute at National University of Singapore and by Department of Physiology at National University of Singapore. NH was supported by JSPS KAKENHI Grant (no. 20K22653). KA was supported by JSPS KAKENHI Grants (no. 19H05798 and no. 22H02625). MM was supported by JSPS KAKENHI Grants (no. 19H00993 and no. 20H05898) and JST Moonshot R&D Grant JPMJPS2022. We appreciate Virgile Viasnoff and the lab members for their valuable comments on the manuscript. We apologize to authors whose work could not be highlighted due to space limitations.","file":[{"content_type":"application/pdf","checksum":"25923f8ae71344e8974530dd23c71bdc","date_updated":"2024-01-30T12:52:12Z","file_name":"2023_CurrentOpinionCellBio_Hirashima.pdf","creator":"dernst","date_created":"2024-01-30T12:52:12Z","file_id":"14909","file_size":1173762,"access_level":"open_access","relation":"main_file","success":1}]},{"volume":136,"quality_controlled":"1","article_number":"jcs260668","abstract":[{"lang":"eng","text":"Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision."}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"publication_status":"published","date_created":"2023-08-20T22:01:13Z","external_id":{"isi":["001070149000001"],"pmid":["37461809"]},"department":[{"_id":"CaHe"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"pmid":1,"acknowledgement":"The authors thank their respective lab members for feedback and helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria for their support.\r\nThis work was supported by the National Institutes of Health [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for Biomedical Engineering Research from the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the European Research Council [advanced grant 742573 to C.-P.H.]. ","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/jcs.260668"}],"author":[{"last_name":"Higashi","full_name":"Higashi, Tomohito","first_name":"Tomohito"},{"first_name":"Rachel E.","full_name":"Stephenson, Rachel E.","last_name":"Stephenson"},{"last_name":"Schwayer","full_name":"Schwayer, Cornelia","first_name":"Cornelia","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226"},{"full_name":"Huljev, Karla","last_name":"Huljev","first_name":"Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Higashi, Atsuko Y.","last_name":"Higashi","first_name":"Atsuko Y."},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Hideki","last_name":"Chiba","full_name":"Chiba, Hideki"},{"first_name":"Ann L.","last_name":"Miller","full_name":"Miller, Ann L."}],"publication":"Journal of Cell Science","scopus_import":"1","isi":1,"intvolume":"       136","ec_funded":1,"_id":"14082","month":"08","title":"ZnUMBA - a live imaging method to detect local barrier breaches","article_processing_charge":"No","citation":{"apa":"Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg, C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>","ama":"Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. 2023;136(15). doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>","ista":"Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ, Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. 136(15), jcs260668.","chicago":"Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev, Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>.","mla":"Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>, vol. 136, no. 15, jcs260668, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>.","short":"T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J. Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).","ieee":"T. Higashi <i>et al.</i>, “ZnUMBA - a live imaging method to detect local barrier breaches,” <i>Journal of Cell Science</i>, vol. 136, no. 15. The Company of Biologists, 2023."},"ddc":["570"],"article_type":"original","OA_type":"free access","doi":"10.1242/jcs.260668","OA_place":"publisher","status":"public","issue":"15","type":"journal_article","day":"01","publisher":"The Company of Biologists","has_accepted_license":"1","date_published":"2023-08-01T00:00:00Z","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"date_updated":"2025-06-25T06:28:45Z","year":"2023","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"ddc":["570"],"doi":"10.1016/j.devcel.2023.02.016","article_type":"original","title":"A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish","month":"04","_id":"12830","citation":{"short":"K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M. Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.","mla":"Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>.","ieee":"K. Huljev <i>et al.</i>, “A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish,” <i>Developmental Cell</i>, vol. 58, no. 7. Elsevier, p. 582–596.e7, 2023.","apa":"Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I., Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>","ama":"Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. 2023;58(7):582-596.e7. doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>","ista":"Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM, Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 58(7), 582–596.e7.","chicago":"Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser, Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>."},"article_processing_charge":"Yes (via OA deal)","year":"2023","oa_version":"Published Version","date_updated":"2025-04-23T08:51:34Z","has_accepted_license":"1","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation","_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017"},{"_id":"266BC5CE-B435-11E9-9278-68D0E5697425","grant_number":"LT000429","name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation"}],"date_published":"2023-04-10T00:00:00Z","page":"582-596.e7","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","type":"journal_article","publisher":"Elsevier","day":"10","issue":"7","publication_status":"published","corr_author":"1","language":[{"iso":"eng"}],"external_id":{"isi":["000982111800001"],"pmid":["36931269"]},"department":[{"_id":"CaHe"},{"_id":"Bio"}],"date_created":"2023-04-16T22:01:07Z","quality_controlled":"1","volume":58,"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"abstract":[{"lang":"eng","text":"Interstitial fluid (IF) accumulation between embryonic cells is thought to be important for embryo patterning and morphogenesis. Here, we identify a positive mechanical feedback loop between cell migration and IF relocalization and find that it promotes embryonic axis formation during zebrafish gastrulation. We show that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between the yolk cell and deep cell tissue to extend the embryonic axis, compress the overlying deep cell layer, thereby causing IF to flow from the deep cell layer to the boundary between the yolk cell and the deep cell layer, directly ahead of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion formation and migration by opening up the space into which the ppl moves and, thereby, the ability of the ppl to trigger IF relocalization by pushing against the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic feedback loop between cell migration and IF relocalization."}],"ec_funded":1,"isi":1,"intvolume":"        58","file_date_updated":"2023-04-17T07:41:25Z","publication":"Developmental Cell","scopus_import":"1","author":[{"first_name":"Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","last_name":"Huljev","full_name":"Huljev, Karla"},{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan","full_name":"Shamipour, Shayan","last_name":"Shamipour"},{"first_name":"Diana C","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","full_name":"Nunes Pinheiro, Diana C","last_name":"Nunes Pinheiro","orcid":"0000-0003-4333-7503"},{"first_name":"Friedrich","last_name":"Preusser","full_name":"Preusser, Friedrich"},{"full_name":"Steccari, Irene","last_name":"Steccari","first_name":"Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","last_name":"Sommer","first_name":"Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-8421-5508","last_name":"Naik","full_name":"Naik, Suyash","first_name":"Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"oa":1,"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"file":[{"file_id":"12842","date_created":"2023-04-17T07:41:25Z","file_name":"2023_DevelopmentalCell_Huljev.pdf","creator":"dernst","file_size":7925886,"checksum":"c80ca2ebc241232aacdb5aa4b4c80957","content_type":"application/pdf","date_updated":"2023-04-17T07:41:25Z","success":1,"relation":"main_file","access_level":"open_access"}],"acknowledgement":"We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful discussions and support with the SPIM experiments; the Heisenberg group, and especially Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work was supported by funding from the European Union (European Research Council Advanced grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des deutschen Volkes to F.P.","pmid":1}]