[{"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"OA_place":"publisher","corr_author":"1","type":"journal_article","quality_controlled":"1","date_published":"2026-01-05T00:00:00Z","publication_identifier":{"issn":["1745-2473"],"issnl":[" 1745-2473"],"eissn":["1745-2481"]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","_id":"21015","date_updated":"2026-04-28T12:55:30Z","file":[{"access_level":"open_access","file_size":7335694,"content_type":"application/pdf","relation":"main_file","file_id":"21026","creator":"dernst","success":1,"file_name":"2026_NaturePhysics_Mishra.pdf","date_updated":"2026-01-21T08:21:11Z","date_created":"2026-01-21T08:21:11Z","checksum":"0ab7ac2fbcb61a364dba57152db64ed7"}],"ddc":["570"],"publication_status":"published","doi":"10.1038/s41567-025-03122-1","intvolume":"        22","volume":22,"external_id":{"oaworkid":["W7118187193"]},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"year":"2026","page":"139-150","OA_type":"hybrid","date_created":"2026-01-20T10:12:19Z","project":[{"name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"},{"name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development","_id":"917c023a-16d5-11f0-9cad-eb5cafc52090"}],"abstract":[{"text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning.","lang":"eng"}],"language":[{"iso":"eng"}],"oaworkid":1,"day":"05","has_accepted_license":"1","PlanS_conform":"1","oa_version":"Published Version","oa":1,"status":"public","acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","article_type":"original","related_material":{"link":[{"relation":"research_data","description":"News on ISTA website","url":"https://ista.ac.at/en/news/geometry-shapes-life/"}]},"author":[{"last_name":"Mishra","full_name":"Mishra, Nikhil","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","orcid":"0000-0002-6425-5788","first_name":"Nikhil"},{"first_name":"Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","last_name":"Li","full_name":"Li, Yuting I"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"month":"01","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","citation":{"ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>."},"ec_funded":1,"publisher":"Springer Nature","publication":"Nature Physics","file_date_updated":"2026-01-21T08:21:11Z","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","article_processing_charge":"Yes (via OA deal)"},{"has_accepted_license":"1","oa_version":"Published Version","oa":1,"status":"public","acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","author":[{"full_name":"Naik, Suyash","last_name":"Naik","first_name":"Suyash","orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87"}],"month":"3","tmp":{"image":"/images/cc_by_sa.png","short":"CC BY-SA (4.0)","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"citation":{"ieee":"S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute of Science and Technology Austria, 2026.","short":"S. Naik, (2026).","apa":"Naik, S. (2026). Data associated with Keratins coordinate tissue spreading . Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>","ama":"Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>","chicago":"Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>.","ista":"Naik S. 2026. Data associated with Keratins coordinate tissue spreading , Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","mla":"Naik, Suyash. <i>Data Associated with Keratins Coordinate Tissue Spreading </i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>."},"ec_funded":1,"publisher":"Institute of Science and Technology Austria","file_date_updated":"2026-03-24T07:21:43Z","title":"Data associated with Keratins coordinate tissue spreading ","article_processing_charge":"No","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"OA_place":"repository","corr_author":"1","type":"research_data","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","date_published":"2026-03-24T00:00:00Z","_id":"21137","date_updated":"2026-06-10T09:44:10Z","contributor":[{"contributor_type":"researcher","first_name":"Yann-Edwin","last_name":"Keta"},{"contributor_type":"supervisor","first_name":"Silke ","last_name":"Henkes"},{"contributor_type":"supervisor","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"},{"last_name":"Hannezo","first_name":"Edouard B","contributor_type":"supervisor","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"}],"file":[{"checksum":"5d1fda7e410f24c311fcf6bcf725698f","date_created":"2026-03-16T11:51:10Z","date_updated":"2026-03-16T11:51:10Z","file_name":"cells-main.zip","title":"Cell git repository","file_id":"21461","creator":"snaik","relation":"main_file","content_type":"application/zip","description":"Python3 library written in C++20 to integrate vertex models. Please read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed instructions for installation and usage of the code in this repository. 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Sasidharan, L. Ancellotti, V. Doddihal, C. Brewster, F. Mann, M.C. McKinney, J. Varberg, E. Ross, F. Deng, K. Yi, A. Sánchez Alvarado, Science Advances 12 (2026).","ieee":"V. Sasidharan <i>et al.</i>, “Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians,” <i>Science Advances</i>, vol. 12, no. 6. American Association for the Advancement of Science, 2026.","ama":"Sasidharan V, Ancellotti L, Doddihal V, et al. Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. <i>Science Advances</i>. 2026;12(6). doi:<a href=\"https://doi.org/10.1126/sciadv.ady1461\">10.1126/sciadv.ady1461</a>","apa":"Sasidharan, V., Ancellotti, L., Doddihal, V., Brewster, C., Mann, F., McKinney, M. C., … Sánchez Alvarado, A. (2026). Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.ady1461\">https://doi.org/10.1126/sciadv.ady1461</a>","mla":"Sasidharan, Vidyanand, et al. “Extracellular Vesicles Mediate Stem Cell Signaling and Systemic RNAi in Planarians.” <i>Science Advances</i>, vol. 12, no. 6, eady1461, American Association for the Advancement of Science, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.ady1461\">10.1126/sciadv.ady1461</a>.","chicago":"Sasidharan, Vidyanand, Laura Ancellotti, Viraj Doddihal, Carolyn Brewster, Frederick Mann, Mary Cathleen McKinney, Joseph Varberg, et al. “Extracellular Vesicles Mediate Stem Cell Signaling and Systemic RNAi in Planarians.” <i>Science Advances</i>. American Association for the Advancement of Science, 2026. <a href=\"https://doi.org/10.1126/sciadv.ady1461\">https://doi.org/10.1126/sciadv.ady1461</a>.","ista":"Sasidharan V, Ancellotti L, Doddihal V, Brewster C, Mann F, McKinney MC, Varberg J, Ross E, Deng F, Yi K, Sánchez Alvarado A. 2026. Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians. Science Advances. 12(6), eady1461."},"tmp":{"short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"scopus_import":"1","file_date_updated":"2026-03-02T14:19:35Z","issue":"6","title":"Extracellular vesicles mediate stem cell signaling and systemic RNAi in planarians","article_processing_charge":"Yes","oa_version":"Published Version","has_accepted_license":"1","acknowledgement":"We thank all the Sánchez Alvarado lab members for inputs and discussions. We are grateful to the Stowers Aquatics (particularly the Planarian team), Microscopy, and Molecular Biology core facilities for technical contributions and method development; e. n. lissek and A. Fujii from Oni US and S. Wang from the University of Missouri, Kansas city, for assistance with dStORM imaging; and d. Alburty and A. Page from innovaprep for assisting with the ntA. We also thank M. Miller for the illustrations. This work was supported by the hhMi and Stowers institute. ","article_type":"original","oa":1,"status":"public","DOAJ_listed":"1","volume":12,"doi":"10.1126/sciadv.ady1461","intvolume":"        12","publication_status":"published","ddc":["570"],"license":"https://creativecommons.org/licenses/by-nc/4.0/","OA_type":"gold","date_created":"2026-03-02T10:08:07Z","year":"2026","day":"01","language":[{"iso":"eng"}],"abstract":[{"text":"Planarian flatworms are known for their remarkable regenerative capacity; however, the precise intercellular communication mechanisms underlying this process remain unsolved. Here, we report the discovery and characterization of abundant extracellular vesicles (EVs) in planarians. Using imaging and molecular analysis, we show conservation of biogenesis, morphology, and protein composition of planarian EVs. Environmental stressors significantly elevate EV release, indicating that planarians dynamically regulate vesicle production. Functionally, planarian EVs mediate intercellular communication by transferring regulatory signals: We find that they shuttle small RNAs that effect systemic RNA interference (RNAi) throughout the organism. Notably, gene knockdown experiments reveal a crucial role for AGO-3, a member of the Argonaute family of proteins, in modulating the association of small interfering RNAs with EVs, linking the intracellular RNAi machinery to EV-based signaling. These findings highlight EVs as pivotal mediators of cell-cell communication in planarians, with broad implications for understanding the coordination of gene regulation and tissue regeneration in animals.","lang":"eng"}],"article_number":"eady1461","type":"journal_article","department":[{"_id":"CaHe"}],"OA_place":"publisher","_id":"21383","file":[{"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":2841345,"date_updated":"2026-03-02T14:19:35Z","file_name":"2026_ScienceAdv_Sasidharan.pdf","checksum":"fa9f6dafe3538e2d2872c098e06d1712","date_created":"2026-03-02T14:19:35Z","file_id":"21389","creator":"dernst","success":1}],"date_updated":"2026-03-02T14:23:22Z","date_published":"2026-02-01T00:00:00Z","publication_identifier":{"eissn":["2375-2548"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1"},{"dataavailabilitystatement":"All data and materials are made available on Zenodo public repository under Creative Commons Attribution 4.0 International License: https://doi.org/10.5281/zenodo.20322020.","article_number":"pgag190","abstract":[{"lang":"eng","text":"Determination of the rheological properties of cells is known to require active measurements, which largely depend on the internalization of mechanical probes. Here, we circumvent this problem via the introduction of Rheological focused light-induced cytoplasmic streaming (Rheo-FLUCS): an active, yet probe-free approach that leverages light-induced flows to access mechanical changes in complex systems. While Rheo-FLUCS is facilitated by thermoviscous expansion phenomena rather than external forces, here we show equivalence in its ability to measure relative viscoelastic properties. Specifically, we demonstrate a phase-lag equivalence with probe-dependent active microrheology in a wide range of physically different, yet chemically identical materials. We exemplify the utility of Rheo-FLUCS in three distinctly different biological systems: compound-treated mouse fibroblasts (NIH-3T3), genetically modified human osteoblasts (U2OS) to elucidate the role of myosins in cytoplasmic mechanics, and early ascidian oocytes of Phallusia mammillata at fertilization stage. Our biological use-cases exemplify the application versatility of Rheo-FLUCS, which in the future may use phase information as a marker for developmental success."}],"language":[{"iso":"eng"}],"day":"01","year":"2026","OA_type":"gold","date_created":"2026-07-13T09:50:12Z","ddc":["570"],"doi":"10.1093/pnasnexus/pgag190","intvolume":"         5","publication_status":"published","external_id":{"biorxivid":["10.1101/2025.04.07.647540"]},"volume":5,"keyword":["cell mechanics","active microrheology","noninvasiveness","thermoviscous flows","FLUCS"],"quality_controlled":"1","date_published":"2026-06-01T00:00:00Z","publication_identifier":{"eissn":["2752-6542"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-07-13T13:14:43Z","file":[{"access_level":"open_access","file_size":3073062,"content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"22312","success":1,"file_name":"2026_PNASNexus_Stoev.pdf","date_updated":"2026-07-13T13:13:56Z","date_created":"2026-07-13T13:13:56Z","checksum":"bb2c89ea73da762ad6423ac299ac3533"}],"_id":"22297","OA_place":"publisher","department":[{"_id":"CaHe"}],"type":"journal_article","biorxivid":1,"supplementarymaterial":"yes","article_processing_charge":"Yes","title":"Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows","file_date_updated":"2026-07-13T13:13:56Z","issue":"6","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","citation":{"ieee":"I. D. Stoev <i>et al.</i>, “Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows,” <i>PNAS Nexus</i>, vol. 5, no. 6. Oxford University Press, 2026.","short":"I.D. Stoev, M. Bolger-Munro, A. Minopoli, S. Wagner, V.R. Krishnaswamy, E. Erben, K. Weißenbruch, N. Maghelli, M. Bastmeyer, C.-P.J. Heisenberg, M. Kreysing, PNAS Nexus 5 (2026).","ama":"Stoev ID, Bolger-Munro M, Minopoli A, et al. Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. 2026;5(6). doi:<a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">10.1093/pnasnexus/pgag190</a>","apa":"Stoev, I. D., Bolger-Munro, M., Minopoli, A., Wagner, S., Krishnaswamy, V. R., Erben, E., … Kreysing, M. (2026). Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">https://doi.org/10.1093/pnasnexus/pgag190</a>","chicago":"Stoev, Iliya D, Madison Bolger-Munro, Antonio Minopoli, Susan Wagner, Venkat Raghavan Krishnaswamy, Elena Erben, Kai Weißenbruch, et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>. Oxford University Press, 2026. <a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">https://doi.org/10.1093/pnasnexus/pgag190</a>.","ista":"Stoev ID, Bolger-Munro M, Minopoli A, Wagner S, Krishnaswamy VR, Erben E, Weißenbruch K, Maghelli N, Bastmeyer M, Heisenberg C-PJ, Kreysing M. 2026. Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. PNAS Nexus. 5(6), pgag190.","mla":"Stoev, Iliya D., et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>, vol. 5, no. 6, pgag190, Oxford University Press, 2026, doi:<a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">10.1093/pnasnexus/pgag190</a>."},"researchdata_availability":"yes","author":[{"first_name":"Iliya D","last_name":"Stoev","full_name":"Stoev, Iliya D"},{"first_name":"Madison","orcid":"0000-0002-8176-4824","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","full_name":"Bolger-Munro, Madison","last_name":"Bolger-Munro"},{"first_name":"Antonio","last_name":"Minopoli","full_name":"Minopoli, Antonio"},{"last_name":"Wagner","full_name":"Wagner, Susan","first_name":"Susan"},{"first_name":"Venkat Raghavan","last_name":"Krishnaswamy","full_name":"Krishnaswamy, Venkat Raghavan"},{"first_name":"Elena","full_name":"Erben, Elena","last_name":"Erben"},{"last_name":"Weißenbruch","full_name":"Weißenbruch, Kai","first_name":"Kai"},{"first_name":"Nicola","last_name":"Maghelli","full_name":"Maghelli, Nicola"},{"last_name":"Bastmeyer","full_name":"Bastmeyer, Martin","first_name":"Martin"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"full_name":"Kreysing, Moritz","last_name":"Kreysing","first_name":"Moritz"}],"month":"06","publication":"PNAS Nexus","publisher":"Oxford University Press","DOAJ_listed":"1","status":"public","oa":1,"article_type":"original","acknowledgement":"The authors gratefully acknowledge help from Dr. Benjamin Seelbinder, Mr. Claudius George, and Mr. Falk Elsner in designing and constructing the magnetic needle for force-driven rheology experiments. We thank Dr. Iain Patten for valuable discussions on the structure of the manuscript and Mr. Ivan Saraev for graphics support. I.D.S. and M.K. kindly acknowledge funding from the Life grant by Volkswagen Foundation (Life! grant no. 92772), Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, Germany's Excellence Strategy, 2082/1 and grant no. 515462906), and the Hector Foundation. I.D.S. was additionally funded by the Karlsruhe Institute of Technology (Excellence Strategy via the Young Investigator Group Preparation Program). V.R.K., S.W., and M.K. thank the ERC Starting Grant GHOSTs (grant no. 853619).","has_accepted_license":"1","das_tickbox":"1","PlanS_conform":"1","oa_version":"Published Version"},{"article_processing_charge":"No","title":"Apical domain mechanosensation regulates tissue tension homeostasis","file_date_updated":"2026-07-13T09:16:28Z","supplementarymaterial":"yes","publisher":"Institute of Science and Technology Austria","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"researchdata_availability":"yes","citation":{"ieee":"N. Hino, T. Kapoor, U. R. Gubbala, E. B. Hannezo, and C.-P. J. Heisenberg, “Apical domain mechanosensation regulates tissue tension homeostasis.” Institute of Science and Technology Austria.","short":"N. Hino, T. Kapoor, U.R. Gubbala, E.B. Hannezo, C.-P.J. Heisenberg, (n.d.).","apa":"Hino, N., Kapoor, T., Gubbala, U. R., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Apical domain mechanosensation regulates tissue tension homeostasis. Institute of Science and Technology Austria.","ama":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","mla":"Hino, Naoya, et al. <i>Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis</i>. Institute of Science and Technology Austria.","ista":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","chicago":"Hino, Naoya, Tushna Kapoor, Uday R Gubbala, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis.” Institute of Science and Technology Austria, n.d."},"author":[{"id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","first_name":"Naoya","full_name":"Hino, Naoya","last_name":"Hino"},{"full_name":"Kapoor, Tushna","last_name":"Kapoor","id":"e3b3eda7-fd4d-11eb-8fd8-c40af7a478b1","first_name":"Tushna"},{"last_name":"Gubbala","full_name":"Gubbala, Uday R","id":"bb4a0dc4-32c9-11ee-b5ce-a97ceedd5924","first_name":"Uday R"},{"orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"relation":"earlier_version","id":"21864","status":"public"}]},"month":"07","acknowledgement":"We thank all members of the Heisenberg group for discussion and feedback on the manuscript, and the Imaging and Optics Facility, the Life Science Support Facility and the Electron Microscopy Facility of the Institute of Science and Technology Austria (ISTA) for their continued support. We are grateful to M. Sonawane (Tata Institute of Fundamental Research, India) for providing the pCS2-HA-aPKC (PKCι)-V260F (DN) and pCS2-HA-aPKC (PKCι)-A122E (CA) plasmids, and to I. Mayer for the discussion. Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. This research was funded in whole or in part by the Austrian Science Fund (FWF; grant no. PAT5044023) to C.-P.H., and by a JSPS Overseas Research Fellowship and an EMBO Postdoctoral Fellowship (ALTF 16-2022) to N.H.","status":"public","oa":1,"oa_version":"Preprint","has_accepted_license":"1","das_tickbox":"1","language":[{"iso":"eng"}],"project":[{"grant_number":"PAT 5044023","name":"Keratins in epithelial tissue spreading","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"_id":"34dd7f3b-11ca-11ed-8bc3-856f2c87f5da","name":"Mechanosensitive signaling activation in the crosstalk between mechanical force and tissuefluidity","grant_number":"LTF 16-2022"}],"abstract":[{"text":"Tissue tension is a key determinant of tissue shape, and its regulation is essential for both morphogenesis and the maintenance of tissue integrity. During zebrafish embryogenesis, the enveloping layer (EVL) – an epithelial monolayer covering the blastoderm – undergoes extensive spreading that is driven by pulling forces exerted at its margin and more than doubles its surface area. Yet whether and how the EVL actively regulates its tissue tension during this process remains unclear. Here, we show that the EVL maintains constant tissue tension while spreading, and that it achieves this by reducing apical cell contractility in response to the same pulling forces that drive its spreading. We identify a mechanosensitive pathway underlying this response, mediated by the scaffold/adaptor protein Kibra regulating the activity of atypical protein kinase C (aPKC) at the apical domain of EVL cells. Under low mechanical stretch, Kibra forms condensates at the base of actin-based apical projections, where it activates Myosin II to increase apical contractility through aPKC downregulation. As mechanical stretch increases, apical projections disassemble, Kibra condensates dissolve, and aPKC activity rises. Elevated aPKC activity in turn reduces apical contractility by reducing Myosin II activity, thereby maintaining constant tissue tension despite increased mechanical stretch. Together, these findings reveal a mechanosensitive mechanism that enables robust adaptation of tissue tension to changing mechanical stretch, ensuring efficient tissue spreading and morphogenesis.","lang":"eng"}],"day":"14","year":"2026","OA_type":"green","date_created":"2026-07-13T09:03:26Z","dataavailabilitystatement":"The MATLAB code for image analysis, and the full model code, including all parameter values\r\nand condition-specific settings, are available on GitHub at https://github.com/uday2607/EVL-tension-homeostasis.git.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"ddc":["570"],"publication_status":"draft","date_updated":"2026-07-14T07:07:41Z","file":[{"creator":"nhino","file_id":"22283","success":1,"file_name":"Main_text_and_figures.pdf","date_updated":"2026-07-13T09:16:20Z","date_created":"2026-07-13T09:16:20Z","checksum":"66444afd243dce7d383d52d44e8d34a4","access_level":"open_access","file_size":12477675,"content_type":"application/pdf","relation":"main_file"},{"date_updated":"2026-07-13T09:16:25Z","file_name":"Supplementary_figures.pdf","checksum":"90bceb34de64ec792c5de117f0890d05","date_created":"2026-07-13T09:16:25Z","creator":"nhino","file_id":"22284","success":1,"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":4545901},{"relation":"main_file","content_type":"video/mp4","file_size":10349451,"access_level":"open_access","checksum":"9d9ab89c372142f2ffb6c8c625334d7f","date_created":"2026-07-13T09:16:28Z","date_updated":"2026-07-13T09:16:28Z","file_name":"Supplementary_Video1.mp4","success":1,"creator":"nhino","file_id":"22285"}],"_id":"22276","keyword":["Epithelial spreading","tissue tension","mechanosensation","aPKC","Kibra","zebrafish"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2026-07-14T00:00:00Z","type":"preprint","corr_author":"1","OA_place":"publisher","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"GradSch"}]},{"month":"03","author":[{"full_name":"Tavano, Ste","last_name":"Tavano","first_name":"Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9970-7804"},{"last_name":"Brückner","full_name":"Brückner, David","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975"},{"full_name":"Tasciyan, Saren","last_name":"Tasciyan","first_name":"Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Xin","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","last_name":"Tong","full_name":"Tong, Xin"},{"id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland","last_name":"Kardos","full_name":"Kardos, Roland"},{"last_name":"Schauer","full_name":"Schauer, Alexandra","first_name":"Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7659-9142"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"citation":{"ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>."},"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"scopus_import":"1","publisher":"Elsevier","isi":1,"publication":"Cell Reports","file_date_updated":"2025-03-17T10:26:54Z","issue":"3","article_processing_charge":"Yes","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","has_accepted_license":"1","oa_version":"Published Version","pmid":1,"oa":1,"status":"public","DOAJ_listed":"1","acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","article_type":"original","doi":"10.1016/j.celrep.2025.115387","publication_status":"published","intvolume":"        44","ddc":["570"],"volume":44,"external_id":{"pmid":["40057955"],"isi":["001443652700001"]},"article_number":"115387","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"OA_type":"gold","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","date_created":"2025-03-16T23:01:24Z","year":"2025","day":"25","project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"},{"_id":"269CD5C4-B435-11E9-9278-68D0E5697425","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","grant_number":"ALTF 1159-2018"}],"abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"language":[{"iso":"eng"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"OA_place":"publisher","corr_author":"1","type":"journal_article","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"date_published":"2025-03-25T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","_id":"19404","file":[{"access_level":"open_access","file_size":9067797,"content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"19413","success":1,"date_updated":"2025-03-17T10:26:54Z","file_name":"2025_CellReports_Tavano.pdf","checksum":"57e05dd1598c807af0afdb32cec039d3","date_created":"2025-03-17T10:26:54Z"}],"date_updated":"2025-10-22T07:00:04Z"},{"article_type":"original","acknowledgement":"We thank all the Heisenberg lab members for discussions and comments on the manuscript, and the Bioimaging and Life Science facilities of ISTA for support with microscopy and fish maintenance, respectively. This study was funded by a Japan Society for the Promotion of Science (JSPS) Overseas Research Fellowship and a Japan Science and Technology Agency PRESTO grant (JPMJPR214B) to Y.M. Open Access funding provided by the Japan Science and Technology Agency. Deposited in PMC for immediate release.","status":"public","oa":1,"pmid":1,"PlanS_conform":"1","oa_version":"Published Version","has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","title":"Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation","issue":"12","file_date_updated":"2025-07-23T08:43:01Z","isi":1,"publication":"Development","publisher":"The Company of Biologists","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","citation":{"ieee":"Y. Moriyama, T. Mitsui, and C.-P. J. Heisenberg, “Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation,” <i>Development</i>, vol. 152, no. 12. The Company of Biologists, 2025.","short":"Y. Moriyama, T. Mitsui, C.-P.J. Heisenberg, Development 152 (2025).","apa":"Moriyama, Y., Mitsui, T., &#38; Heisenberg, C.-P. J. (2025). Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.204261\">https://doi.org/10.1242/dev.204261</a>","ama":"Moriyama Y, Mitsui T, Heisenberg C-PJ. Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. <i>Development</i>. 2025;152(12). doi:<a href=\"https://doi.org/10.1242/dev.204261\">10.1242/dev.204261</a>","mla":"Moriyama, Yuuta, et al. “Hoxb Genes Determine the Timing of Cell Ingression by Regulating Cell Surface Fluctuations during Zebrafish Gastrulation.” <i>Development</i>, vol. 152, no. 12, dev204261, The Company of Biologists, 2025, doi:<a href=\"https://doi.org/10.1242/dev.204261\">10.1242/dev.204261</a>.","chicago":"Moriyama, Yuuta, Toshiyuki Mitsui, and Carl-Philipp J Heisenberg. “Hoxb Genes Determine the Timing of Cell Ingression by Regulating Cell Surface Fluctuations during Zebrafish Gastrulation.” <i>Development</i>. The Company of Biologists, 2025. <a href=\"https://doi.org/10.1242/dev.204261\">https://doi.org/10.1242/dev.204261</a>.","ista":"Moriyama Y, Mitsui T, Heisenberg C-PJ. 2025. Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation. Development. 152(12), dev204261."},"author":[{"orcid":"0000-0002-2853-8051","id":"addc9b8c-67a0-11f0-b374-a2e094825470","first_name":"Yuuta","last_name":"Moriyama","full_name":"Moriyama, Yuuta"},{"first_name":"Toshiyuki","last_name":"Mitsui","full_name":"Mitsui, Toshiyuki"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"month":"06","date_updated":"2025-09-30T14:07:51Z","file":[{"date_created":"2025-07-23T08:43:01Z","checksum":"808d8aa28df79d23fb661838d1fdc1be","file_name":"2025_Development_Moriyama.pdf","date_updated":"2025-07-23T08:43:01Z","success":1,"creator":"dernst","file_id":"20070","relation":"main_file","content_type":"application/pdf","file_size":25935563,"access_level":"open_access"}],"_id":"20048","quality_controlled":"1","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"date_published":"2025-06-27T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","corr_author":"1","OA_place":"publisher","department":[{"_id":"CaHe"}],"abstract":[{"lang":"eng","text":"During embryonic development, cell behaviors need to be tightly regulated in time and space. Yet how the temporal and spatial regulations of cell behaviors are interconnected during embryonic development remains elusive. To address this, we turned to zebrafish gastrulation, the process whereby dynamic cell behaviors generate the three principal germ layers of the early embryo. Here, we show that Hoxb cluster genes are expressed in a temporally collinear manner at the blastoderm margin, where mesodermal and endodermal (mesendoderm) progenitor cells are specified and ingress to form mesendoderm/hypoblast. Functional analysis shows that these Hoxb genes regulate the timing of cell ingression: under- or overexpression of Hoxb genes perturb the timing of mesendoderm cell ingression and, consequently, the positioning of these cells along the forming anterior-posterior body axis after gastrulation. Finally, we found that Hoxb genes control the timing of mesendoderm ingression by regulating cellular bleb formation and cell surface fluctuations in the ingressing cells. Collectively, our findings suggest that Hoxb genes interconnect the temporal and spatial pattern of cell behaviors during zebrafish gastrulation by controlling cell surface fluctuations."}],"language":[{"iso":"eng"}],"day":"27","year":"2025","OA_type":"hybrid","date_created":"2025-07-21T08:10:32Z","article_number":"dev204261","external_id":{"isi":["001525252300001"],"pmid":["40576478"]},"volume":152,"ddc":["570"],"publication_status":"published","intvolume":"       152","doi":"10.1242/dev.204261"},{"file_date_updated":"2025-09-01T09:46:44Z","title":"Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo","article_processing_charge":"Yes","publisher":"Springer Nature","publication":"Nature Communications","month":"08","author":[{"first_name":"Ioannis","last_name":"Segos","full_name":"Segos, Ioannis"},{"first_name":"Jens","full_name":"Van Eeckhoven, Jens","last_name":"Van Eeckhoven"},{"last_name":"Berger","full_name":"Berger, Simon","first_name":"Simon"},{"first_name":"Nikhil","orcid":"0000-0002-6425-5788","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","last_name":"Mishra","full_name":"Mishra, Nikhil"},{"first_name":"Eric J.","full_name":"Lambie, Eric J.","last_name":"Lambie"},{"first_name":"Barbara","full_name":"Conradt, Barbara","last_name":"Conradt"}],"citation":{"chicago":"Segos, Ioannis, Jens Van Eeckhoven, Simon Berger, Nikhil Mishra, Eric J. Lambie, and Barbara Conradt. “Unequal Segregation of Mitochondria during Asymmetric Cell Division Contributes to Cell Fate Divergence in Sister Cells in Vivo.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-62484-5\">https://doi.org/10.1038/s41467-025-62484-5</a>.","mla":"Segos, Ioannis, et al. “Unequal Segregation of Mitochondria during Asymmetric Cell Division Contributes to Cell Fate Divergence in Sister Cells in Vivo.” <i>Nature Communications</i>, vol. 16, 7174, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-62484-5\">10.1038/s41467-025-62484-5</a>.","ista":"Segos I, Van Eeckhoven J, Berger S, Mishra N, Lambie EJ, Conradt B. 2025. Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. Nature Communications. 16, 7174.","ieee":"I. Segos, J. Van Eeckhoven, S. Berger, N. Mishra, E. J. Lambie, and B. Conradt, “Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","short":"I. Segos, J. Van Eeckhoven, S. Berger, N. Mishra, E.J. Lambie, B. Conradt, Nature Communications 16 (2025).","apa":"Segos, I., Van Eeckhoven, J., Berger, S., Mishra, N., Lambie, E. J., &#38; Conradt, B. (2025). Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-62484-5\">https://doi.org/10.1038/s41467-025-62484-5</a>","ama":"Segos I, Van Eeckhoven J, Berger S, Mishra N, Lambie EJ, Conradt B. Unequal segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in sister cells in vivo. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-62484-5\">10.1038/s41467-025-62484-5</a>"},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","acknowledgement":"We thank members of the Conradt lab, the Center for Cell and Molecular Dynamics (https://www.uclccmd.co.uk/) and T. Schedl for discussions and comments on the manuscript. We thank L. McGuinness for excellent technical support. Some strains were provided by the Caenorhabditis Genetics Center (CGC), which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We thank Alex Hajnal (University of Zurich, Switzerland) and Andrew deMello (ETH Zurich, Switzerland) for their support of S.B. This work was supported by a predoctoral fellowship from the Studienstiftung des deutschen Volkes to NM, funds from UCL (Division of Biosciences, UCL LSM Capital Equipment Fund) to B.C., and a Wolfson Fellowship from the Royal Society (https://royalsociety.org/) to B.C. (RSWF\\R1\\180008), and the Biotechnology and Biological Sciences Research Council (https://bbsrc.ukri.org/) (BB/V007572/1 and BB/V015648/1to B.C.).","article_type":"original","oa":1,"status":"public","DOAJ_listed":"1","oa_version":"Published Version","PlanS_conform":"1","pmid":1,"has_accepted_license":"1","OA_type":"gold","date_created":"2025-08-17T22:01:35Z","year":"2025","day":"04","language":[{"iso":"eng"}],"abstract":[{"text":"The unequal segregation of organelles has been proposed to be an intrinsic mechanism that contributes to cell fate divergence during asymmetric cell division; however, in vivo evidence is sparse. Using super-resolution microscopy, we analysed the segregation of organelles during the division of the neuroblast QL.p in C. elegans larvae. QL.p divides to generate a daughter that survives, QL.pa, and a daughter that dies, QL.pp. We found that mitochondria segregate unequally by density and morphology and that this is dependent on mitochondrial dynamics. Furthermore, we found that mitochondrial density in QL.pp correlates with the time it takes QL.pp to die. We propose that low mitochondrial density in QL.pp promotes the cell death fate and ensures that QL.pp dies in a highly reproducible and timely manner. Our results provide in vivo evidence that the unequal segregation of mitochondria can contribute to cell fate divergence during asymmetric cell division in a developing animal.","lang":"eng"}],"article_number":"7174","volume":16,"external_id":{"pmid":["40759648"]},"intvolume":"        16","doi":"10.1038/s41467-025-62484-5","publication_status":"published","ddc":["570"],"_id":"20183","file":[{"access_level":"open_access","file_size":3775190,"content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"20261","success":1,"file_name":"2025_NatureComm_Segos.pdf","date_updated":"2025-09-01T09:46:44Z","date_created":"2025-09-01T09:46:44Z","checksum":"f28e73963ea1f55876d0d1afca0f706a"}],"date_updated":"2025-09-01T09:47:29Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["2041-1723"]},"date_published":"2025-08-04T00:00:00Z","quality_controlled":"1","type":"journal_article","department":[{"_id":"CaHe"}],"OA_place":"publisher"},{"status":"public","oa":1,"article_type":"original","acknowledgement":"We are grateful to the members of the Matsuda Laboratory for their helpful input, to K. Hirano, T. Uesugi and K. Takakura, who provided technical assistance, and to the Medical Research Support Center of Kyoto University for DNA sequence analysis. This work was supported by the Kyoto University Live Imaging Center. Financial support was provided by Japan Society for the Promotion of Science (JSPS) KAKENHI grants (21H05226 to K.T., 19H00993 and 20H05898 to M.M.), a Japan Science and Technology Agency (JST) CREST grant (JPMJCR1654 to M.M.), and a JST Moonshot Research and Development Program grant (JPMJPS2022 to M.M.). Open Access funding provided by Tokushima University. Deposited in PMC for immediate release.","has_accepted_license":"1","pmid":1,"PlanS_conform":"1","oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","title":"Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration","issue":"15","file_date_updated":"2025-09-01T10:02:24Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","citation":{"ista":"Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K. 2025. Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. Journal of Cell Science. 138(15), 263779.","chicago":"Jikko, Yuya, Eriko Deguchi, Kimiya Matsuda, Naoya Hino, Shinya Tsukiji, Michiyuki Matsuda, and Kenta Terai. “Front-Biased Activation of the Ras-Rab5-Rac1 Loop Coordinates Collective Cell Migration.” <i>Journal of Cell Science</i>. The Company of Biologists, 2025. <a href=\"https://doi.org/10.1242/jcs.263779\">https://doi.org/10.1242/jcs.263779</a>.","mla":"Jikko, Yuya, et al. “Front-Biased Activation of the Ras-Rab5-Rac1 Loop Coordinates Collective Cell Migration.” <i>Journal of Cell Science</i>, vol. 138, no. 15, 263779, The Company of Biologists, 2025, doi:<a href=\"https://doi.org/10.1242/jcs.263779\">10.1242/jcs.263779</a>.","apa":"Jikko, Y., Deguchi, E., Matsuda, K., Hino, N., Tsukiji, S., Matsuda, M., &#38; Terai, K. (2025). Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.263779\">https://doi.org/10.1242/jcs.263779</a>","ama":"Jikko Y, Deguchi E, Matsuda K, et al. Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration. <i>Journal of Cell Science</i>. 2025;138(15). doi:<a href=\"https://doi.org/10.1242/jcs.263779\">10.1242/jcs.263779</a>","ieee":"Y. Jikko <i>et al.</i>, “Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration,” <i>Journal of Cell Science</i>, vol. 138, no. 15. The Company of Biologists, 2025.","short":"Y. Jikko, E. Deguchi, K. Matsuda, N. Hino, S. Tsukiji, M. Matsuda, K. Terai, Journal of Cell Science 138 (2025)."},"author":[{"first_name":"Yuya","last_name":"Jikko","full_name":"Jikko, Yuya"},{"first_name":"Eriko","last_name":"Deguchi","full_name":"Deguchi, Eriko"},{"first_name":"Kimiya","full_name":"Matsuda, Kimiya","last_name":"Matsuda"},{"full_name":"Hino, Naoya","last_name":"Hino","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","first_name":"Naoya"},{"first_name":"Shinya","full_name":"Tsukiji, Shinya","last_name":"Tsukiji"},{"first_name":"Michiyuki","last_name":"Matsuda","full_name":"Matsuda, Michiyuki"},{"first_name":"Kenta","full_name":"Terai, Kenta","last_name":"Terai"}],"month":"08","isi":1,"publication":"Journal of Cell Science","publisher":"The Company of Biologists","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1477-9137"],"issn":[" 0021-9533"]},"date_published":"2025-08-01T00:00:00Z","date_updated":"2025-11-27T14:12:24Z","file":[{"relation":"main_file","content_type":"application/pdf","file_size":12393297,"access_level":"open_access","checksum":"29f42619dab5ce251a20c769ed4581c0","date_created":"2025-09-01T10:02:24Z","date_updated":"2025-09-01T10:02:24Z","file_name":"2025_JourCellScience_Jikko.pdf","success":1,"creator":"dernst","file_id":"20262"}],"_id":"20188","OA_place":"publisher","department":[{"_id":"CaHe"}],"type":"journal_article","article_number":"263779","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Collective cell migration is coordinated by the front-to-rear intercellular propagation of EGFR-Ras-ERK pathway activation. However, the molecular mechanisms integrating front-to-rear information into this intercellular signaling cascade, particularly the determinants of cellular front-side specification, remain elusive. We visualized the activity of EGFR, Ras, Rac1 and Rab5A (hereafter Rab5) by using FRET biosensors and chemogenetic tools. Whereas EGFR activation was uniformly observed within cells, Ras activation was biased to the front side within cells. The polarized Ras activation depended on Merlin and Rac1, which also showed front-biased activation. Furthermore, Rab5, a crucial regulator of cell migration, demonstrated similar front-biased activation and was found to function downstream of Ras while being necessary for Rac1 activation. Thus, the positive feedback loop consisting of Ras, Rab5 and Rac1 is activated primarily at the front of collectively migrating cells. These findings offer new spatio-temporal insight into processing front–rear information during collective cell migration."}],"day":"01","year":"2025","date_created":"2025-08-17T22:01:36Z","OA_type":"hybrid","ddc":["570"],"doi":"10.1242/jcs.263779","publication_status":"published","intvolume":"       138","external_id":{"isi":["001567723900009"],"pmid":["40667649"]},"volume":138},{"volume":175,"external_id":{"pmid":["40913907"],"isi":["001567260100001"]},"doi":"10.1016/j.semcdb.2025.103650","publication_status":"published","intvolume":"       175","ddc":["570"],"date_created":"2025-09-14T22:01:32Z","OA_type":"hybrid","year":"2025","day":"01","abstract":[{"text":"Oogenesis – the formation and development of an oocyte – is fundamental to reproduction and embryonic development. Due to its accessibility to genetic manipulations and the ability to culture and experimentally manipulate oocytes ex vivo, zebrafish has emerged as a powerful vertebrate model system for studying oogenesis. In this review, we provide a comprehensive overview of zebrafish oogenesis, from early germ cell formation to oocyte maturation and fertilization. We discuss recent advances in uncovering the molecular and cellular mechanisms driving this complex process and highlight key knowledge gaps that remain to be addressed.","lang":"eng"}],"language":[{"iso":"eng"}],"article_number":"103650","corr_author":"1","type":"journal_article","department":[{"_id":"CaHe"}],"OA_place":"publisher","_id":"20349","file":[{"access_level":"open_access","file_size":2778561,"content_type":"application/pdf","relation":"main_file","file_id":"20914","creator":"dernst","success":1,"file_name":"2025_SemCellDevBiology_Hofmann.pdf","date_updated":"2025-12-30T10:21:00Z","date_created":"2025-12-30T10:21:00Z","checksum":"80ea6cbb004853bb1e87db3422a74aca"}],"date_updated":"2025-12-30T10:21:13Z","publication_identifier":{"eissn":["1096-3634"],"issn":["1084-9521"]},"date_published":"2025-12-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","publisher":"Elsevier","isi":1,"publication":"Seminars in Cell and Developmental Biology","month":"12","author":[{"last_name":"Hofmann","full_name":"Hofmann, Laura","first_name":"Laura","id":"b88d43f2-dc74-11ea-a0a7-e41b7912e031"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"citation":{"apa":"Hofmann, L., &#38; Heisenberg, C.-P. J. (2025). Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. <i>Seminars in Cell and Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">https://doi.org/10.1016/j.semcdb.2025.103650</a>","ama":"Hofmann L, Heisenberg C-PJ. Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. <i>Seminars in Cell and Developmental Biology</i>. 2025;175. doi:<a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">10.1016/j.semcdb.2025.103650</a>","short":"L. Hofmann, C.-P.J. Heisenberg, Seminars in Cell and Developmental Biology 175 (2025).","ieee":"L. Hofmann and C.-P. J. Heisenberg, “Decoding zebrafish oogenesis: From primordial germ cell development to fertilization,” <i>Seminars in Cell and Developmental Biology</i>, vol. 175. Elsevier, 2025.","ista":"Hofmann L, Heisenberg C-PJ. 2025. Decoding zebrafish oogenesis: From primordial germ cell development to fertilization. Seminars in Cell and Developmental Biology. 175, 103650.","mla":"Hofmann, Laura, and Carl-Philipp J. Heisenberg. “Decoding Zebrafish Oogenesis: From Primordial Germ Cell Development to Fertilization.” <i>Seminars in Cell and Developmental Biology</i>, vol. 175, 103650, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">10.1016/j.semcdb.2025.103650</a>.","chicago":"Hofmann, Laura, and Carl-Philipp J Heisenberg. “Decoding Zebrafish Oogenesis: From Primordial Germ Cell Development to Fertilization.” <i>Seminars in Cell and Developmental Biology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.semcdb.2025.103650\">https://doi.org/10.1016/j.semcdb.2025.103650</a>."},"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2025-12-30T10:21:00Z","title":"Decoding zebrafish oogenesis: From primordial germ cell development to fertilization","article_processing_charge":"Yes (via OA deal)","oa_version":"Published Version","pmid":1,"PlanS_conform":"1","has_accepted_license":"1","acknowledgement":"We thank Carolina Camelo for making schematics for this review.","article_type":"review","oa":1,"status":"public"},{"article_processing_charge":"No","title":"Keratins act as global coordinators of tissue spreading through mechanosensitive feedback","file_date_updated":"2025-10-28T13:10:26Z","tmp":{"image":"/images/cc_by_sa.png","short":"CC BY-SA (4.0)","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"citation":{"ista":"Naik S. 2025. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. Institute of Science and Technology Austria.","chicago":"Naik, Suyash. “Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>.","mla":"Naik, Suyash. <i>Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>.","apa":"Naik, S. (2025). <i>Keratins act as global coordinators of tissue spreading through mechanosensitive feedback</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>","ama":"Naik S. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>","ieee":"S. Naik, “Keratins act as global coordinators of tissue spreading through mechanosensitive feedback,” Institute of Science and Technology Austria, 2025.","short":"S. Naik, Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback, Institute of Science and Technology Austria, 2025."},"author":[{"orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash","last_name":"Naik","full_name":"Naik, Suyash"}],"related_material":{"record":[{"id":"20465","relation":"part_of_dissertation","status":"public"}]},"month":"10","publisher":"Institute of Science and Technology Austria","status":"public","oa":1,"acknowledgement":"I would also like to thank the LSF and Cryo facility at ISTA, which have been helpful in my\r\nexperiments. I would also like to acknowledge FWF, grant DOI 10.55776/PAT5044023 and JKU Nanocell grant DOI \r\n10.55776/W1250 for providing funding for my PhD research. EMBO and FWF for providing funding for travel grants to attend conferences.","has_accepted_license":"1","oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"degree_awarded":"PhD","language":[{"iso":"eng"}],"project":[{"_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023","name":"Keratins in epithelial tissue spreading"},{"grant_number":"W 1250-B20","call_identifier":"FWF","name":"Nano-Analytics of Cellular Systems","_id":"25AA5F24-B435-11E9-9278-68D0E5697425"}],"abstract":[{"text":"Epithelial spreading plays a pivotal role in the development of organisms especially those\r\nsuch as zebrafish which require the epithelial enveloping layer (EVL) to spread to cover the\r\nsubstantial yolk surface during gastrulation. Epiboly requires the transition of the epithelium\r\nwith cuboidal cells to form a thin, flat squamous epithelial sheet. During this transition, the\r\ncells show tissue-scale mechanosensation with mechanisms such as direct mechanical control\r\nover the axis of cell division.\r\nCytoskeletal intermediate filaments play a crucial role in vertebrate cells, not only facilitating\r\nmechanical stability but also helping facilitate the mechanosensitive response of the cell.\r\nMechanosenstivity displayed by intermediate filaments is due not just to their interesting\r\nphysical properties but also to their interactions with other cytoskeletal elements such as actin\r\nand microtubules. Keratin is the predominant intermediate filament expressed in the EVL.\r\nIt expresses concomitantly with the gastrulation movements of the developing embryo. Our\r\nwork focuses on understanding the role and dynamics of the keratin cytoskeletal network in\r\nmodulating the physical aspects of EVL spreading. We demonstrated with the combination of\r\nphysical characterisation and manipulations of the EVL, utilising a variety of biophysical tools\r\nand microscopy, the mechanistic role of keratin in tissue spreading.\r\nGenerating novel genetic morphants and mutants, we probe the effect that the loss of the\r\nkeratin network has on the physiology of the epithelium and the developing embryo. We\r\nshow that the changing organisation of the keratin network is important for changing EVL\r\nphysical properties as the stress imposed on the EVL increases during epiboly. By modelling\r\nthe epithelium, we study how the mechanical heterogeneity in an epithelium can feed back into\r\na mechanical loop to the maturation of the keratin network and hence affect the mechanics\r\nof the epithelium. However, unlike what would be predicted by the effect of intermediate\r\nfilaments in acting as a security belt and increasing the resistance of the epithelium, we observe\r\nthat loss of keratin leads to a delay in the EVL movement. Using both local aspirations of the\r\nYSL and EVL ablations, we demonstrate the mechanistic facilitation of actin mechanosensation\r\nin a keratin-dependent manner.\r\nFurthermore, using chemical inhibitors of microtubule polymerisation, we provide insight into\r\nthe mechanisms underlying the organisation and distribution of keratin. Interestingly, the\r\nphenotype observed upon this loss of microtubules shows that keratins interact with the nucleus\r\nthrough microtubular interactions. Together with these diverse observations, we describe\r\nthe mechanosensory feedback between resilience and that is critical for uniform and robust\r\nspreading of the epithelium.","lang":"eng"}],"alternative_title":["ISTA Thesis"],"day":"12","year":"2025","date_created":"2025-10-10T14:58:30Z","page":"105","ddc":["596","597","532"],"doi":"10.15479/AT-ISTA-20441","publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"isbn":["978-3-99078-069-5"],"issn":["2663-337X"]},"date_published":"2025-10-12T00:00:00Z","date_updated":"2026-07-06T12:51:41Z","file":[{"file_id":"20567","creator":"snaik","success":1,"date_updated":"2025-10-28T13:10:08Z","file_name":"Thesis_PDFA_.pdf","checksum":"2892f04d4a5c18677871c3e06ac1244a","date_created":"2025-10-28T13:10:08Z","access_level":"open_access","file_size":6846189,"content_type":"application/pdf","relation":"main_file"},{"file_id":"20568","creator":"snaik","checksum":"15934d4465cd0e9b7c32678da9a33a2f","date_created":"2025-10-28T13:10:26Z","date_updated":"2025-10-28T13:10:26Z","file_name":"Thesis.zip","file_size":8839300,"access_level":"open_access","relation":"source_file","content_type":"application/zip"}],"_id":"20441","OA_place":"publisher","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"type":"dissertation","corr_author":"1","supervisor":[{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}]},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"date_published":"2025-02-17T00:00:00Z","status":"public","_id":"20465","date_updated":"2026-07-06T12:51:42Z","das_tickbox":"1","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"OA_place":"repository","oa_version":"Preprint","corr_author":"1","type":"preprint","date_created":"2025-10-14T07:25:27Z","license":"https://creativecommons.org/licenses/by-nd/4.0/","year":"2025","day":"17","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","article_processing_charge":"No","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"For tissues to spread, they must be deformable while maintaining their structural integrity. How these opposing requirements are balanced within spreading tissues is not yet well understood. Here, we show that keratin intermediate filaments function in epithelial spreading by adapting tissue mechanical resilience to the stresses arising in the tissue during the spreading process. By analysing the expansion of the enveloping cell layer (EVL) over the large yolk cell in early zebrafish embryos in vivo, we found that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, which is essential for preventing tissue rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the force-generating processes pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production in the yolk cell and the mechanical resilience of the EVL against stresses generated by these pulling forces, thereby ensuring uniform and robust tissue spreading."}],"publication_status":"draft","doi":"10.1101/2025.02.14.638262","month":"02","related_material":{"record":[{"id":"20441","relation":"dissertation_contains","status":"public"}]},"author":[{"full_name":"Naik, Suyash","last_name":"Naik","first_name":"Suyash","orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Keta","full_name":"Keta, Yann-Edwin","first_name":"Yann-Edwin"},{"full_name":"Pranjic-Ferscha, Kornelija","last_name":"Pranjic-Ferscha","first_name":"Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Silke","full_name":"Henkes, Silke","last_name":"Henkes"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"citation":{"ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>.","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>bioRxiv</i>. .","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, BioRxiv (n.d.)."},"tmp":{"name":"Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nd/4.0/legalcode","short":"CC BY-ND (4.0)","image":"/image/cc_by_nd.png"},"main_file_link":[{"url":"https://doi.org/10.1101/2025.02.14.638262","open_access":"1"}],"publication":"bioRxiv"},{"external_id":{"pmid":["38134934"],"isi":["001154500400001"]},"volume":34,"ddc":["570"],"intvolume":"        34","publication_status":"published","doi":"10.1016/j.cub.2023.11.067","project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"}],"language":[{"iso":"eng"}],"abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"day":"08","year":"2024","page":"171-182.e8","date_created":"2024-01-14T23:00:56Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"type":"journal_article","corr_author":"1","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"date_updated":"2025-09-04T11:39:10Z","file":[{"file_name":"2024_CurrentBiology_Arslan.pdf","date_updated":"2024-01-16T10:53:31Z","date_created":"2024-01-16T10:53:31Z","checksum":"51220b76d72a614208f84bdbfbaf9b72","file_id":"14813","creator":"dernst","success":1,"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":5183861}],"_id":"14795","quality_controlled":"1","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"date_published":"2024-01-08T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"publication":"Current Biology","publisher":"Elsevier","ec_funded":1,"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>.","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>"},"author":[{"last_name":"Arslan","full_name":"Arslan, Feyza N","orcid":"0000-0001-5809-9566","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","first_name":"Feyza N"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack"},{"first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7309-9724","last_name":"Loose","full_name":"Loose, Martin"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"month":"01","article_processing_charge":"Yes (via OA deal)","title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","file_date_updated":"2024-01-16T10:53:31Z","issue":"1","pmid":1,"oa_version":"Published Version","has_accepted_license":"1","article_type":"original","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","status":"public","oa":1},{"quality_controlled":"1","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"date_published":"2024-02-01T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"14846","date_updated":"2025-09-04T11:48:28Z","file":[{"success":1,"file_id":"17267","creator":"dernst","checksum":"7891ebe7c900ae47469ab127031dd1ec","date_created":"2024-07-16T12:12:43Z","date_updated":"2024-07-16T12:12:43Z","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","file_size":9897883,"access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"corr_author":"1","type":"journal_article","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"year":"2024","date_created":"2024-01-21T23:00:57Z","page":"310-321","abstract":[{"text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces.","lang":"eng"}],"language":[{"iso":"eng"}],"project":[{"_id":"2646861A-B435-11E9-9278-68D0E5697425","name":"Control of embryonic cleavage pattern","call_identifier":"FWF","grant_number":"I03601"}],"day":"01","ddc":["530"],"doi":"10.1038/s41567-023-02302-1","publication_status":"published","intvolume":"        20","volume":20,"external_id":{"isi":["001138880800005"],"pmid":["38370025"]},"oa":1,"status":"public","acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","article_type":"original","has_accepted_license":"1","pmid":1,"oa_version":"Published Version","file_date_updated":"2024-07-16T12:12:43Z","article_processing_charge":"Yes (in subscription journal)","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","related_material":{"link":[{"relation":"press_release","description":"News on ISTA Website","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/"}]},"author":[{"full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5223-3346"},{"last_name":"Shinde","full_name":"Shinde, Rushikesh","first_name":"Rushikesh"},{"first_name":"Madison","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","orcid":"0000-0002-8176-4824","last_name":"Bolger-Munro","full_name":"Bolger-Munro, Madison"},{"first_name":"Matilda","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3415-4628","last_name":"Peruzzo","full_name":"Peruzzo, Matilda"},{"full_name":"Szep, Gregory","last_name":"Szep","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory"},{"first_name":"Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","last_name":"Steccari","full_name":"Steccari, Irene"},{"id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","first_name":"David","full_name":"Labrousse Arias, David","last_name":"Labrousse Arias"},{"last_name":"Zheden","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9438-4783","first_name":"Vanessa"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","last_name":"Merrin"},{"full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones","first_name":"Andrew"},{"last_name":"Voituriez","full_name":"Voituriez, Raphaël","first_name":"Raphaël"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"month":"02","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024."},"publisher":"Springer Nature","isi":1,"publication":"Nature Physics"},{"file":[{"relation":"main_file","content_type":"application/pdf","file_size":14839986,"access_level":"open_access","checksum":"6961ea10012bf0d266681f9628bb8f13","date_created":"2024-03-04T07:24:43Z","date_updated":"2024-03-04T07:24:43Z","file_name":"2024_Development_Schauer.pdf","success":1,"creator":"dernst","file_id":"15050"}],"date_updated":"2025-09-04T12:10:40Z","_id":"15048","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"date_published":"2024-02-01T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","type":"journal_article","corr_author":"1","department":[{"_id":"CaHe"},{"_id":"Bio"}],"day":"01","language":[{"iso":"eng"}],"project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425"}],"abstract":[{"text":"Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play key roles in germ layer specification and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants to study the coordination of Nodal and BMP signaling for embryo patterning and morphogenesis. We show that Nodal signaling triggers explant elongation by inducing mesendodermal progenitors but also suppressing BMP signaling activity at the site of mesendoderm induction. Consistent with this, ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm intercalations, key processes during explant elongation. Translating these ex vivo observations to the intact embryo showed that, similar to explants, Nodal signaling suppresses the effect of BMP signaling on cell intercalations in the dorsal domain, thus allowing robust embryonic axis elongation. These findings suggest a dual function of Nodal signaling in embryonic axis elongation by both inducing mesendoderm and suppressing BMP effects in the dorsal portion of the mesendoderm.","lang":"eng"}],"page":"1-18","date_created":"2024-03-03T23:00:50Z","year":"2024","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"external_id":{"isi":["001170580200001"],"pmid":["38372390"]},"volume":151,"intvolume":"       151","publication_status":"published","doi":"10.1242/dev.202316","ddc":["570"],"article_type":"original","acknowledgement":"We thank Patrick Müller for sharing the chordintt250 mutant zebrafish line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro and Katherine Rogers and members of the Heisenberg lab for discussions, technical advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo for discussions. We thank the Imaging and Optics Facility as well as the Life Science facility at IST Austria for support with microscopy and fish maintenance.\r\nThis work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573 to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience and Technology Austria. ","status":"public","oa":1,"oa_version":"Published Version","pmid":1,"has_accepted_license":"1","title":"Robust axis elongation by Nodal-dependent restriction of BMP signaling","article_processing_charge":"Yes (via OA deal)","issue":"4","file_date_updated":"2024-03-04T07:24:43Z","isi":1,"publication":"Development","ec_funded":1,"publisher":"The Company of Biologists","citation":{"apa":"Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J. (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>","ama":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18. doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>","short":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development 151 (2024) 1–18.","ieee":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>, vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.","chicago":"Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>.","mla":"Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, The Company of Biologists, 2024, pp. 1–18, doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>.","ista":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4), 1–18."},"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"month":"02","related_material":{"record":[{"relation":"research_data","id":"14926","status":"public"}]},"author":[{"full_name":"Schauer, Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7659-9142","first_name":"Alexandra"},{"id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}]},{"citation":{"ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","month":"05","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/"}]},"author":[{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926","first_name":"Lukas","last_name":"Hörmayer","full_name":"Hörmayer, Lukas"},{"last_name":"Montesinos López","full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C"},{"full_name":"Trozzi, N","last_name":"Trozzi","first_name":"N"},{"last_name":"Spona","full_name":"Spona, Leonhard","id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard"},{"full_name":"Yoshida, Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko"},{"full_name":"Marhavá, Petra","last_name":"Marhavá","id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra"},{"full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","first_name":"Silvia","orcid":"0000-0002-5223-3346","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Y","full_name":"Dagdas, Y","last_name":"Dagdas"},{"last_name":"Majda","full_name":"Majda, M","first_name":"M"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml"}],"publication":"Developmental Cell","isi":1,"ec_funded":1,"publisher":"Elsevier","title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","article_processing_charge":"Yes (via OA deal)","issue":"10","file_date_updated":"2024-08-20T11:22:16Z","has_accepted_license":"1","oa_version":"Published Version","pmid":1,"status":"public","oa":1,"article_type":"original","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","publication_status":"published","intvolume":"        59","doi":"10.1016/j.devcel.2024.03.009","ddc":["570"],"external_id":{"pmid":["38579717"],"isi":["001301584600001"]},"volume":59,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"day":"20","language":[{"iso":"eng"}],"project":[{"grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"grant_number":"P29988","call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development","_id":"262EF96E-B435-11E9-9278-68D0E5697425"}],"abstract":[{"lang":"eng","text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture."}],"date_created":"2024-04-08T12:07:57Z","page":"1333-1344.e4","year":"2024","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"type":"journal_article","corr_author":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-05-20T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"quality_controlled":"1","file":[{"date_created":"2024-08-20T11:22:16Z","checksum":"22b374fb50a40d380b7686c84258d271","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","date_updated":"2024-08-20T11:22:16Z","success":1,"creator":"dernst","file_id":"17452","relation":"main_file","content_type":"application/pdf","file_size":5195262,"access_level":"open_access"}],"date_updated":"2025-09-04T13:32:08Z","_id":"15301"},{"status":"public","article_type":"letter_note","oa_version":"None","pmid":1,"issue":"24","article_processing_charge":"No","title":"Development: Turing mechanics","month":"12","author":[{"first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","full_name":"Hino, Naoya","last_name":"Hino"},{"id":"6347dca5-074c-11ed-af92-a80f860d9d5b","first_name":"Carolina","last_name":"Santos Fernandes Lasbarrères Camelo","full_name":"Santos Fernandes Lasbarrères Camelo, Carolina"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"citation":{"ista":"Hino N, Santos Fernandes Lasbarrères Camelo C, Heisenberg C-PJ. 2024. Development: Turing mechanics. Current Biology. 34(24), R1230–R1232.","mla":"Hino, Naoya, et al. “Development: Turing Mechanics.” <i>Current Biology</i>, vol. 34, no. 24, Elsevier, 2024, pp. R1230–32, doi:<a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">10.1016/j.cub.2024.10.065</a>.","chicago":"Hino, Naoya, Carolina Santos Fernandes Lasbarrères Camelo, and Carl-Philipp J Heisenberg. “Development: Turing Mechanics.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">https://doi.org/10.1016/j.cub.2024.10.065</a>.","ama":"Hino N, Santos Fernandes Lasbarrères Camelo C, Heisenberg C-PJ. Development: Turing mechanics. <i>Current Biology</i>. 2024;34(24):R1230-R1232. doi:<a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">10.1016/j.cub.2024.10.065</a>","apa":"Hino, N., Santos Fernandes Lasbarrères Camelo, C., &#38; Heisenberg, C.-P. J. (2024). Development: Turing mechanics. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2024.10.065\">https://doi.org/10.1016/j.cub.2024.10.065</a>","short":"N. Hino, C. Santos Fernandes Lasbarrères Camelo, C.-P.J. Heisenberg, Current Biology 34 (2024) R1230–R1232.","ieee":"N. Hino, C. Santos Fernandes Lasbarrères Camelo, and C.-P. J. Heisenberg, “Development: Turing mechanics,” <i>Current Biology</i>, vol. 34, no. 24. Elsevier, pp. R1230–R1232, 2024."},"scopus_import":"1","publisher":"Elsevier","isi":1,"publication":"Current Biology","date_published":"2024-12-16T00:00:00Z","publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","_id":"18651","date_updated":"2025-09-09T11:51:15Z","department":[{"_id":"CaHe"}],"corr_author":"1","type":"journal_article","OA_type":"closed access","date_created":"2024-12-15T23:01:49Z","page":"R1230-R1232","year":"2024","day":"16","abstract":[{"lang":"eng","text":"Embryo axis formation begins with the localized expression of biochemical signals, which organize cell movements and determine cell fate. A quail study finds that tissue contraction and resulting long-range changes in tissue tension restrict the area where these biochemical signals are expressed."}],"language":[{"iso":"eng"}],"intvolume":"        34","doi":"10.1016/j.cub.2024.10.065","publication_status":"published","volume":34,"external_id":{"pmid":["39689690"],"isi":["001392077000001"]}},{"publication":"eLife","publisher":"eLife Sciences Publications","citation":{"apa":"Knabl, P., Schauer, A., Pomreinke, A. P., Zimmermann, B., Rogers, K. W., Čapek, D., … Genikhovich, G. (2024). Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>","ama":"Knabl P, Schauer A, Pomreinke AP, et al. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>eLife</i>. 2024;13. doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>","short":"P. Knabl, A. Schauer, A.P. Pomreinke, B. Zimmermann, K.W. Rogers, D. Čapek, P. Müller, G. Genikhovich, ELife 13 (2024).","ieee":"P. Knabl <i>et al.</i>, “Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator,” <i>eLife</i>, vol. 13. eLife Sciences Publications, 2024.","mla":"Knabl, Paul, et al. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>, vol. 13, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.80803\">10.7554/elife.80803</a>.","chicago":"Knabl, Paul, Alexandra Schauer, Autumn P Pomreinke, Bob Zimmermann, Katherine W Rogers, Daniel Čapek, Patrick Müller, and Grigory Genikhovich. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.80803\">https://doi.org/10.7554/elife.80803</a>.","ista":"Knabl P, Schauer A, Pomreinke AP, Zimmermann B, Rogers KW, Čapek D, Müller P, Genikhovich G. 2024. Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. eLife. 13."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","month":"02","author":[{"first_name":"Paul","last_name":"Knabl","full_name":"Knabl, Paul"},{"first_name":"Alexandra","orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","full_name":"Schauer, Alexandra","last_name":"Schauer"},{"first_name":"Autumn P","full_name":"Pomreinke, Autumn P","last_name":"Pomreinke"},{"full_name":"Zimmermann, Bob","last_name":"Zimmermann","first_name":"Bob"},{"first_name":"Katherine W","full_name":"Rogers, Katherine W","last_name":"Rogers"},{"last_name":"Čapek","full_name":"Čapek, Daniel","first_name":"Daniel"},{"first_name":"Patrick","full_name":"Müller, Patrick","last_name":"Müller"},{"full_name":"Genikhovich, Grigory","last_name":"Genikhovich","first_name":"Grigory"}],"title":"Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator","article_processing_charge":"Yes","file_date_updated":"2025-01-29T08:50:18Z","oa_version":"Published Version","has_accepted_license":"1","article_type":"original","acknowledgement":"This work was funded by the Austrian Science Foundation (FWF) grants P26962-B21 and P32705-B to GG and by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 637840 [QUANTPATTERN] and 863952 [ACE-OF-SPACE]) to PM. We thank Michaela Schwaiger, Taras Kreslavsky, Hiromi Tagoh, and Patricio Ferrer Murguia for their help with the ChIP protocol, Matthias Richter and Christian Hofer for their assistance with in situ analyses, Emilio Gonzalez Morales for making the measurements for Figure 6—figure supplement 3, Catrin Weiler for the assistance in cloning zebrafish zswim5, David Mörsdorf for critically reading the manuscript and help with data visualization, and the Core Facility for Cell Imaging and Ultrastructure Research of the University of Vienna for access to the confocal microscope.","status":"public","DOAJ_listed":"1","oa":1,"volume":13,"publication_status":"published","intvolume":"        13","doi":"10.7554/elife.80803","ddc":["570"],"day":"07","abstract":[{"text":"BMP signaling has a conserved function in patterning the dorsal-ventral body axis in Bilateria and the directive axis in anthozoan cnidarians. So far, cnidarian studies have focused on the role of different BMP signaling network components in regulating pSMAD1/5 gradient formation. Much less is known about the target genes downstream of BMP signaling. To address this, we generated a genome-wide list of direct pSMAD1/5 target genes in the anthozoan <jats:italic>Nematostella vectensis</jats:italic>, several of which were conserved in <jats:italic>Drosophila</jats:italic> and <jats:italic>Xenopus</jats:italic>. Our ChIP-seq analysis revealed that many of the regulatory molecules with documented bilaterally symmetric expression in <jats:italic>Nematostella</jats:italic> are directly controlled by BMP signaling. We identified several so far uncharacterized BMP-dependent transcription factors and signaling molecules, whose bilaterally symmetric expression may be indicative of their involvement in secondary axis patterning. One of these molecules is <jats:italic>zswim4-6</jats:italic>, which encodes a novel nuclear protein that can modulate the pSMAD1/5 gradient and potentially promote BMP-dependent gene repression.","lang":"eng"}],"language":[{"iso":"eng"}],"date_created":"2025-01-29T08:48:34Z","OA_type":"gold","year":"2024","type":"journal_article","OA_place":"publisher","department":[{"_id":"CaHe"}],"file":[{"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":11855972,"file_name":"2024_eLife_Knabl.pdf","date_updated":"2025-01-29T08:50:18Z","date_created":"2025-01-29T08:50:18Z","checksum":"24548a184215d3f4547bba535ccfd7b1","file_id":"18941","creator":"dernst","success":1}],"date_updated":"2025-01-29T08:56:21Z","_id":"18940","date_published":"2024-02-07T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["2050-084X"]},"quality_controlled":"1"},{"publication":"Developmental Cell","isi":1,"ec_funded":1,"publisher":"Elsevier","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"mla":"Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>.","chicago":"Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser, Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>.","ista":"Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM, Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 58(7), 582–596.e7.","short":"K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M. Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.","ieee":"K. Huljev <i>et al.</i>, “A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish,” <i>Developmental Cell</i>, vol. 58, no. 7. Elsevier, p. 582–596.e7, 2023.","ama":"Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. 2023;58(7):582-596.e7. doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>","apa":"Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I., Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>"},"author":[{"first_name":"Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","full_name":"Huljev, Karla","last_name":"Huljev"},{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan","full_name":"Shamipour, Shayan","last_name":"Shamipour"},{"first_name":"Diana C","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4333-7503","last_name":"Nunes Pinheiro","full_name":"Nunes Pinheiro, Diana C"},{"last_name":"Preusser","full_name":"Preusser, Friedrich","first_name":"Friedrich"},{"full_name":"Steccari, Irene","last_name":"Steccari","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene"},{"last_name":"Sommer","full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M"},{"orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash","last_name":"Naik","full_name":"Naik, Suyash"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"month":"04","article_processing_charge":"Yes (via OA deal)","title":"A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish","file_date_updated":"2023-04-17T07:41:25Z","issue":"7","pmid":1,"oa_version":"Published Version","has_accepted_license":"1","article_type":"original","acknowledgement":"We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful discussions and support with the SPIM experiments; the Heisenberg group, and especially Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work was supported by funding from the European Union (European Research Council Advanced grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des deutschen Volkes to F.P.","status":"public","oa":1,"external_id":{"isi":["000982111800001"],"pmid":["36931269"]},"volume":58,"ddc":["570"],"publication_status":"published","intvolume":"        58","doi":"10.1016/j.devcel.2023.02.016","language":[{"iso":"eng"}],"abstract":[{"text":"Interstitial fluid (IF) accumulation between embryonic cells is thought to be important for embryo patterning and morphogenesis. Here, we identify a positive mechanical feedback loop between cell migration and IF relocalization and find that it promotes embryonic axis formation during zebrafish gastrulation. We show that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between the yolk cell and deep cell tissue to extend the embryonic axis, compress the overlying deep cell layer, thereby causing IF to flow from the deep cell layer to the boundary between the yolk cell and the deep cell layer, directly ahead of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion formation and migration by opening up the space into which the ppl moves and, thereby, the ability of the ppl to trigger IF relocalization by pushing against the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic feedback loop between cell migration and IF relocalization.","lang":"eng"}],"project":[{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation","_id":"26520D1E-B435-11E9-9278-68D0E5697425"},{"_id":"266BC5CE-B435-11E9-9278-68D0E5697425","name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation","grant_number":"LT000429"}],"day":"10","year":"2023","page":"582-596.e7","date_created":"2023-04-16T22:01:07Z","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"type":"journal_article","corr_author":"1","department":[{"_id":"CaHe"},{"_id":"Bio"}],"date_updated":"2025-04-23T08:51:34Z","file":[{"creator":"dernst","file_id":"12842","success":1,"file_name":"2023_DevelopmentalCell_Huljev.pdf","date_updated":"2023-04-17T07:41:25Z","date_created":"2023-04-17T07:41:25Z","checksum":"c80ca2ebc241232aacdb5aa4b4c80957","access_level":"open_access","file_size":7925886,"content_type":"application/pdf","relation":"main_file"}],"_id":"12830","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2023-04-10T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]}},{"project":[{"grant_number":"742573","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425"}],"abstract":[{"lang":"eng","text":"Dynamic reorganization of the cytoplasm is key to many core cellular processes, such as cell division, cell migration, and cell polarization. Cytoskeletal rearrangements are thought to constitute the main drivers of cytoplasmic flows and reorganization. In contrast, remarkably little is known about how dynamic changes in size and shape of cell organelles affect cytoplasmic organization. Here, we show that within the maturing zebrafish oocyte, the surface localization of exocytosis-competent cortical granules (Cgs) upon germinal vesicle breakdown (GVBD) is achieved by the combined activities of yolk granule (Yg) fusion and microtubule aster formation and translocation. We find that Cgs are moved towards the oocyte surface through radially outward cytoplasmic flows induced by Ygs fusing and compacting towards the oocyte center in response to GVBD. We further show that vesicles decorated with the small Rab GTPase Rab11, a master regulator of vesicular trafficking and exocytosis, accumulate together with Cgs at the oocyte surface. This accumulation is achieved by Rab11-positive vesicles being transported by acentrosomal microtubule asters, the formation of which is induced by the release of CyclinB/Cdk1 upon GVBD, and which display a net movement towards the oocyte surface by preferentially binding to the oocyte actin cortex. We finally demonstrate that the decoration of Cgs by Rab11 at the oocyte surface is needed for Cg exocytosis and subsequent chorion elevation, a process central in egg activation. Collectively, these findings unravel a yet unrecognized role of organelle fusion, functioning together with cytoskeletal rearrangements, in orchestrating cytoplasmic organization during oocyte maturation."}],"language":[{"iso":"eng"}],"day":"08","year":"2023","date_created":"2023-07-16T22:01:09Z","page":"e3002146","ddc":["570"],"intvolume":"        21","publication_status":"published","doi":"10.1371/journal.pbio.3002146","external_id":{"pmid":["37289834"],"isi":["001003199100005"]},"volume":21,"quality_controlled":"1","date_published":"2023-06-08T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"eissn":["1545-7885"]},"date_updated":"2025-04-14T07:46:59Z","file":[{"content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":4431723,"file_name":"2023_PloSBiology_Shamipour.pdf","date_updated":"2023-07-18T07:59:58Z","date_created":"2023-07-18T07:59:58Z","checksum":"8e88cb0e5a6433a2f1939a9030bed384","file_id":"13246","creator":"dernst","success":1}],"_id":"13229","department":[{"_id":"CaHe"}],"type":"journal_article","corr_author":"1","title":"Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes","article_processing_charge":"No","file_date_updated":"2023-07-18T07:59:58Z","issue":"6","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","citation":{"ista":"Shamipour S, Hofmann L, Steccari I, Kardos R, Heisenberg C-PJ. 2023. Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. PLoS Biology. 21(6), e3002146.","mla":"Shamipour, Shayan, et al. “Yolk Granule Fusion and Microtubule Aster Formation Regulate Cortical Granule Translocation and Exocytosis in Zebrafish Oocytes.” <i>PLoS Biology</i>, vol. 21, no. 6, Public Library of Science, 2023, p. e3002146, doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002146\">10.1371/journal.pbio.3002146</a>.","chicago":"Shamipour, Shayan, Laura Hofmann, Irene Steccari, Roland Kardos, and Carl-Philipp J Heisenberg. “Yolk Granule Fusion and Microtubule Aster Formation Regulate Cortical Granule Translocation and Exocytosis in Zebrafish Oocytes.” <i>PLoS Biology</i>. Public Library of Science, 2023. <a href=\"https://doi.org/10.1371/journal.pbio.3002146\">https://doi.org/10.1371/journal.pbio.3002146</a>.","ieee":"S. Shamipour, L. Hofmann, I. Steccari, R. Kardos, and C.-P. J. Heisenberg, “Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes,” <i>PLoS Biology</i>, vol. 21, no. 6. Public Library of Science, p. e3002146, 2023.","short":"S. Shamipour, L. Hofmann, I. Steccari, R. Kardos, C.-P.J. Heisenberg, PLoS Biology 21 (2023) e3002146.","ama":"Shamipour S, Hofmann L, Steccari I, Kardos R, Heisenberg C-PJ. Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. <i>PLoS Biology</i>. 2023;21(6):e3002146. doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002146\">10.1371/journal.pbio.3002146</a>","apa":"Shamipour, S., Hofmann, L., Steccari, I., Kardos, R., &#38; Heisenberg, C.-P. J. (2023). Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.3002146\">https://doi.org/10.1371/journal.pbio.3002146</a>"},"author":[{"first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","last_name":"Shamipour"},{"first_name":"Laura","id":"b88d43f2-dc74-11ea-a0a7-e41b7912e031","full_name":"Hofmann, Laura","last_name":"Hofmann"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","last_name":"Steccari","full_name":"Steccari, Irene"},{"full_name":"Kardos, Roland","last_name":"Kardos","id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"month":"06","publication":"PLoS Biology","isi":1,"publisher":"Public Library of Science","ec_funded":1,"status":"public","oa":1,"article_type":"original","acknowledgement":"This work was supported by funding from the European Union (European Research Council Advanced grant 742573) to C.-P.H. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","has_accepted_license":"1","pmid":1,"oa_version":"Published Version"}]