[{"intvolume":"        15","external_id":{"isi":["000312841300007"]},"issue":"1","oa_version":"None","_id":"2918","status":"public","acknowledgement":"This work was supported by the SNSF, the Swiss SystemsX.ch initiative and LipidX-2008/011 (M.G-G. and F.G.v.d.G.), by the Fondation SANTE-Vaduz/Aide au Soutien des Nouvelles Thérapies (F.G.v.d.G.) and by the ERC, the NCCR Frontiers in Genetics and Chemical Biology programmes and the Polish–Swiss research program (M.G-G.).","year":"2013","citation":{"apa":"Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.-P. J., Van Der Goot, F., &#38; González Gaitán, M. (2013). Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>","ieee":"I. Castanon, L. Abrami, L. Holtzer, C.-P. J. Heisenberg, F. Van Der Goot, and M. González Gaitán, “Anthrax toxin receptor 2a controls mitotic spindle positioning,” <i>Nature Cell Biology</i>, vol. 15, no. 1. Nature Publishing Group, pp. 28–39, 2013.","ista":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. 2013. Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. 15(1), 28–39.","ama":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. 2013;15(1):28-39. doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>","mla":"Castanon, Irinka, et al. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>, vol. 15, no. 1, Nature Publishing Group, 2013, pp. 28–39, doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>.","short":"I. Castanon, L. Abrami, L. Holtzer, C.-P.J. Heisenberg, F. Van Der Goot, M. González Gaitán, Nature Cell Biology 15 (2013) 28–39.","chicago":"Castanon, Irinka, Laurence Abrami, Laurent Holtzer, Carl-Philipp J Heisenberg, Françoise Van Der Goot, and Marcos González Gaitán. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>."},"publisher":"Nature Publishing Group","day":"01","isi":1,"fulldoi":"https://doi.org/10.1038/ncb2632","date_published":"2013-01-01T00:00:00Z","date_updated":"2025-09-30T08:16:16Z","publist_id":"3819","abstract":[{"lang":"eng","text":"Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.\r\n"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T12:00:20Z","article_processing_charge":"No","quality_controlled":"1","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":15,"publication_status":"published","page":"28 - 39","month":"01","doi":"10.1038/ncb2632","type":"journal_article","title":"Anthrax toxin receptor 2a controls mitotic spindle positioning","scopus_import":"1","author":[{"full_name":"Castanon, Irinka","last_name":"Castanon","first_name":"Irinka"},{"last_name":"Abrami","full_name":"Abrami, Laurence","first_name":"Laurence"},{"first_name":"Laurent","last_name":"Holtzer","full_name":"Holtzer, Laurent"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"},{"full_name":"Van Der Goot, Françoise","last_name":"Van Der Goot","first_name":"Françoise"},{"full_name":"González Gaitán, Marcos","last_name":"González Gaitán","first_name":"Marcos"}],"publication":"Nature Cell Biology"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Cell polarisation in development is a common and fundamental process underlying embryo patterning and morphogenesis, and has been extensively studied over the past years. Our current knowledge of cell polarisation in development is predominantly based on studies that have analysed polarisation of single cells, such as eggs, or cellular aggregates with a stable polarising interface, such as cultured epithelial cells (St Johnston and Ahringer, 2010). However, in embryonic development, particularly of vertebrates, cell polarisation processes often encompass large numbers of cells that are placed within moving and proliferating tissues, and undergo mesenchymal-to-epithelial transitions with a highly complex spatiotemporal choreography. How such intricate cell polarisation processes in embryonic development are achieved has only started to be analysed. By using live imaging of neurulation in the transparent zebrafish embryo, Buckley et al (2012) now describe a novel polarisation strategy by which cells assemble an apical domain in the part of their cell body that intersects with the midline of the forming neural rod. This mechanism, along with the previously described mirror-symmetric divisions (Tawk et al, 2007), is thought to trigger formation of both neural rod midline and lumen."}],"publist_id":"3817","date_updated":"2025-09-29T13:27:27Z","date_published":"2013-01-09T00:00:00Z","fulldoi":"https://doi.org/10.1038/emboj.2012.325","day":"09","isi":1,"year":"2013","citation":{"ista":"Compagnon J, Heisenberg C-PJ. 2013. Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. 32(1), 1–3.","ama":"Compagnon J, Heisenberg C-PJ. Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. 2013;32(1):1-3. doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>","ieee":"J. Compagnon and C.-P. J. Heisenberg, “Neurulation coordinating cell polarisation and lumen formation,” <i>EMBO Journal</i>, vol. 32, no. 1. Wiley-Blackwell, pp. 1–3, 2013.","apa":"Compagnon, J., &#38; Heisenberg, C.-P. J. (2013). Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>","chicago":"Compagnon, Julien, and Carl-Philipp J Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>.","mla":"Compagnon, Julien, and Carl-Philipp J. Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>, vol. 32, no. 1, Wiley-Blackwell, 2013, pp. 1–3, doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>.","short":"J. Compagnon, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 1–3."},"publisher":"Wiley-Blackwell","_id":"2920","status":"public","oa_version":"Submitted Version","external_id":{"isi":["000314141900001"],"pmid":["23211745"]},"issue":"1","intvolume":"        32","publication":"EMBO Journal","author":[{"first_name":"Julien","last_name":"Compagnon","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","full_name":"Compagnon, Julien"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"corr_author":"1","scopus_import":"1","pmid":1,"title":"Neurulation coordinating cell polarisation and lumen formation","oa":1,"doi":"10.1038/emboj.2012.325","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545307/","open_access":"1"}],"type":"journal_article","month":"01","page":"1 - 3","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":32,"publication_status":"published","quality_controlled":"1","date_created":"2018-12-11T12:00:20Z","article_processing_charge":"No"},{"fulldoi":"https://doi.org/10.1016/j.cub.2012.11.034","date_published":"2013-01-07T00:00:00Z","date_updated":"2026-04-08T14:22:39Z","publist_id":"3811","ec_funded":1,"abstract":[{"text":"To fight infectious diseases, host immune defenses are employed at multiple levels. Sanitary behavior, such as pathogen avoidance and removal, acts as a first line of defense to prevent infection [1] before activation of the physiological immune system. Insect societies have evolved a wide range of collective hygiene measures and intensive health care toward pathogen-exposed group members [2]. One of the most common behaviors is allogrooming, in which nestmates remove infectious particles from the body surfaces of exposed individuals [3]. Here we show that, in invasive garden ants, grooming of fungus-exposed brood is effective beyond the sheer mechanical removal of fungal conidiospores; it also includes chemical disinfection through the application of poison produced by the ants themselves. Formic acid is the main active component of the poison. It inhibits fungal growth of conidiospores remaining on the brood surface after grooming and also those collected in the mouth of the grooming ant. This dual function is achieved by uptake of the poison droplet into the mouth through acidopore self-grooming and subsequent application onto the infectious brood via brood grooming. This extraordinary behavior extends the current understanding of grooming and the establishment of social immunity in insect societies.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"        23","external_id":{"isi":["000313383700026"]},"issue":"1","oa_version":"None","_id":"2926","status":"public","year":"2013","acknowledgement":"Funding for this project was obtained by the German Research Foundation (DFG, to S.C.) and the European Research Council (ERC, through an ERC-Starting Grant to S.C. and an Individual Marie Curie IEF fellowship to L.V.U.).\r\nWe thank Jørgen Eilenberg, Bernhardt Steinwender, Miriam Stock, and Meghan L. Vyleta for the fungal strain and its characterization; Volker Witte for chemical information; Eva Sixt for ant drawings; and Robert Hauschild for help with image analysis. We further thank Martin Kaltenpoth, Michael Sixt, Jürgen Heinze, and Joachim Ruther for discussion and Daria Siekhaus, Sophie A.O. Armitage, and Leila Masri for comments on the manuscript. \r\n","citation":{"chicago":"Tragust, Simon, Barbara Mitteregger, Vanessa Barone, Matthias Konrad, Line V Ugelvig, and Sylvia Cremer. “Ants Disinfect Fungus-Exposed Brood by Oral Uptake and Spread of Their Poison.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">https://doi.org/10.1016/j.cub.2012.11.034</a>.","mla":"Tragust, Simon, et al. “Ants Disinfect Fungus-Exposed Brood by Oral Uptake and Spread of Their Poison.” <i>Current Biology</i>, vol. 23, no. 1, Cell Press, 2013, pp. 76–82, doi:<a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">10.1016/j.cub.2012.11.034</a>.","short":"S. Tragust, B. Mitteregger, V. Barone, M. Konrad, L.V. Ugelvig, S. Cremer, Current Biology 23 (2013) 76–82.","ista":"Tragust S, Mitteregger B, Barone V, Konrad M, Ugelvig LV, Cremer S. 2013. Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. Current Biology. 23(1), 76–82.","ama":"Tragust S, Mitteregger B, Barone V, Konrad M, Ugelvig LV, Cremer S. Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. <i>Current Biology</i>. 2013;23(1):76-82. doi:<a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">10.1016/j.cub.2012.11.034</a>","apa":"Tragust, S., Mitteregger, B., Barone, V., Konrad, M., Ugelvig, L. V., &#38; Cremer, S. (2013). Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">https://doi.org/10.1016/j.cub.2012.11.034</a>","ieee":"S. Tragust, B. Mitteregger, V. Barone, M. Konrad, L. V. Ugelvig, and S. Cremer, “Ants disinfect fungus-exposed brood by oral uptake and spread of their poison,” <i>Current Biology</i>, vol. 23, no. 1. Cell Press, pp. 76–82, 2013."},"publisher":"Cell Press","day":"07","isi":1,"doi":"10.1016/j.cub.2012.11.034","type":"journal_article","title":"Ants disinfect fungus-exposed brood by oral uptake and spread of their poison","scopus_import":"1","corr_author":"1","author":[{"first_name":"Simon","full_name":"Tragust, Simon","last_name":"Tragust","id":"35A7A418-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Mitteregger","id":"479DDAAC-E9CD-11E9-9B5F-82450873F7A1","full_name":"Mitteregger, Barbara","first_name":"Barbara"},{"full_name":"Barone, Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa","orcid":"0000-0003-2676-3367"},{"first_name":"Matthias","last_name":"Konrad","id":"46528076-F248-11E8-B48F-1D18A9856A87","full_name":"Konrad, Matthias"},{"orcid":"0000-0003-1832-8883","first_name":"Line V","last_name":"Ugelvig","id":"3DC97C8E-F248-11E8-B48F-1D18A9856A87","full_name":"Ugelvig, Line V"},{"first_name":"Sylvia","orcid":"0000-0002-2193-3868","full_name":"Cremer, Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer"}],"publication":"Current Biology","date_created":"2018-12-11T12:00:23Z","article_processing_charge":"No","quality_controlled":"1","related_material":{"record":[{"relation":"research_data","id":"9757","status":"public"},{"status":"public","relation":"dissertation_contains","id":"961"}]},"project":[{"grant_number":"CR-118/3-1","_id":"25DAF0B2-B435-11E9-9278-68D0E5697425","name":"Host-Parasite Coevolution"},{"name":"Social Vaccination in Ant Colonies: from Individual Mechanisms to Society Effects","_id":"25DC711C-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"243071"},{"grant_number":"302004","call_identifier":"FP7","name":"Collective disease defence and pathogen detection abilities in ant societies: a chemo-neuro-immunological approach","_id":"25DDF0F0-B435-11E9-9278-68D0E5697425"}],"volume":23,"language":[{"iso":"eng"}],"department":[{"_id":"SyCr"},{"_id":"CaHe"}],"publication_status":"published","page":"76 - 82","month":"01"},{"alternative_title":["ISTA Thesis"],"date_updated":"2026-07-29T10:00:09Z","publist_id":"5801","corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","author":[{"full_name":"Campinho, Pedro","last_name":"Campinho","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","first_name":"Pedro","orcid":"0000-0002-8526-5416"}],"abstract":[{"text":"Epithelial spreading is a critical part of various developmental and wound repair processes. Here we use zebrafish epiboly as a model system to study the cellular and molecular mechanisms underlying the spreading of epithelial sheets. During zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the embryo to eventually cover the entire yolk cell by the end of gastrulation. The EVL leading edge is anchored through tight junctions to the yolk syncytial layer (YSL), where directly adjacent to the EVL margin a contractile actomyosin ring is formed that is thought to drive EVL epiboly. The prevalent view in the field was that the contractile ring exerts a pulling force on the EVL margin, which pulls the EVL towards the vegetal pole. However, how this force is generated and how it affects EVL morphology still remains elusive. Moreover, the cellular mechanisms mediating the increase in EVL surface area, while maintaining tissue integrity and function are still unclear. Here we show that the YSL actomyosin ring pulls on the EVL margin by two distinct force-generating mechanisms. One mechanism is based on contraction of the ring around its circumference, as previously proposed. The second mechanism is based on actomyosin retrogade flows, generating force through resistance against the substrate. The latter can function at any epiboly stage even in situations where the contraction-based mechanism is unproductive. Additionally, we demonstrate that during epiboly the EVL is subjected to anisotropic tension, which guides the orientation of EVL cell division along the main axis (animal-vegetal) of tension. The influence of tension in cell division orientation involves cell elongation and requires myosin-2 activity for proper spindle alignment. Strikingly, we reveal that tension-oriented cell divisions release anisotropic tension within the EVL and that in the absence of such divisions, EVL cells undergo ectopic fusions. We conclude that forces applied to the EVL by the action of the YSL actomyosin ring generate a tension anisotropy in the EVL that orients cell divisions, which in turn limit tissue tension increase thereby facilitating tissue spreading.","lang":"eng"}],"title":"Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading","date_published":"2013-10-01T00:00:00Z","publication_identifier":{"issn":["2663-337X"]},"type":"dissertation","OA_place":"publisher","doi_confirm":"1","_id":"1406","page":"123","status":"public","oa_version":"None","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"GradSch"}],"language":[{"iso":"eng"}],"supervisor":[{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"month":"10","day":"01","publisher":"Institute of Science and Technology Austria","citation":{"ama":"Campinho P. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. 2013.","ista":"Campinho P. 2013. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. Institute of Science and Technology Austria.","ieee":"P. Campinho, “Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading,” Institute of Science and Technology Austria, 2013.","apa":"Campinho, P. (2013). <i>Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading</i>. Institute of Science and Technology Austria.","chicago":"Campinho, Pedro. “Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading.” Institute of Science and Technology Austria, 2013.","short":"P. Campinho, Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading, Institute of Science and Technology Austria, 2013.","mla":"Campinho, Pedro. <i>Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading</i>. Institute of Science and Technology Austria, 2013."},"year":"2013","article_processing_charge":"No","date_created":"2018-12-11T11:51:50Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"degree_awarded":"PhD"},{"abstract":[{"text":"Epithelial spreading is a common and fundamental aspect of various developmental and disease-related processes such as epithelial closure and wound healing. A key challenge for epithelial tissues undergoing spreading is to increase their surface area without disrupting epithelial integrity. Here we show that orienting cell divisions by tension constitutes an efficient mechanism by which the enveloping cell layer (EVL) releases anisotropic tension while undergoing spreading during zebrafish epiboly. The control of EVL cell-division orientation by tension involves cell elongation and requires myosin II activity to align the mitotic spindle with the main tension axis. We also found that in the absence of tension-oriented cell divisions and in the presence of increased tissue tension, EVL cells undergo ectopic fusions, suggesting that the reduction of tension anisotropy by oriented cell divisions is required to prevent EVL cells from fusing. We conclude that cell-division orientation by tension constitutes a key mechanism for limiting tension anisotropy and thus promoting tissue spreading during EVL epiboly.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publist_id":"4652","date_updated":"2026-07-29T10:07:18Z","fulldoi":"https://doi.org/10.1038/ncb2869","date_published":"2013-11-10T00:00:00Z","publisher":"Nature Publishing Group","acknowledgement":"This work was supported by the IST Austria and MPI-CBG ","citation":{"ama":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. 2013;15:1405-1414. doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>","ista":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.","apa":"Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., &#38; Heisenberg, C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>","ieee":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg, “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly,” <i>Nature Cell Biology</i>, vol. 15. Nature Publishing Group, pp. 1405–1414, 2013.","chicago":"Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc, and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>.","mla":"Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>.","short":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg, Nature Cell Biology 15 (2013) 1405–1414."},"year":"2013","isi":1,"day":"10","oa_version":"Submitted Version","status":"public","_id":"2282","intvolume":"        15","external_id":{"isi":["000327944200005"]},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"author":[{"first_name":"Pedro","orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","last_name":"Campinho"},{"first_name":"Martin","full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ranft","full_name":"Ranft, Jonas","first_name":"Jonas"},{"first_name":"Thomas","last_name":"Risler","full_name":"Risler, Thomas"},{"full_name":"Minc, Nicolas","last_name":"Minc","first_name":"Nicolas"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"publication":"Nature Cell Biology","scopus_import":"1","corr_author":"1","type":"journal_article","doi":"10.1038/ncb2869","main_file_link":[{"url":"http://hal.upmc.fr/hal-00983313/","open_access":"1"}],"oa":1,"title":"Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly","month":"11","publication_status":"published","language":[{"iso":"eng"}],"volume":15,"department":[{"_id":"CaHe"}],"page":"1405 - 1414","quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1403"}]},"project":[{"name":"Control of Epithelial Cell Layer Spreading in Zebrafish","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I930-B20"}],"article_processing_charge":"No","date_created":"2018-12-11T11:56:45Z"},{"department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":338,"publication_status":"published","page":"253 - 256","month":"10","date_created":"2018-12-11T12:00:31Z","article_processing_charge":"No","quality_controlled":"1","scopus_import":"1","corr_author":"1","author":[{"orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître"},{"first_name":"Hélène","last_name":"Berthoumieux","full_name":"Berthoumieux, Hélène"},{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"last_name":"Julicher","full_name":"Julicher, Frank","first_name":"Frank"},{"full_name":"Paluch, Ewa","last_name":"Paluch","first_name":"Ewa"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"publication":"Science","doi":"10.1126/science.1225399","type":"journal_article","title":"Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells","oa_version":"None","_id":"2951","status":"public","year":"2012","citation":{"ieee":"J.-L. Maître <i>et al.</i>, “Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells,” <i>Science</i>, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 253–256, 2012.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. 2012;338(6104):253-256. doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>","ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 338(6104), 253–256.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Science 338 (2012) 253–256.","mla":"Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 253–56, doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>.","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>."},"publisher":"American Association for the Advancement of Science","day":"12","isi":1,"acknowledged_ssus":[{"_id":"SSU"}],"intvolume":"       338","external_id":{"isi":["000309712300045"]},"issue":"6104","publist_id":"3777","date_updated":"2025-09-30T08:10:36Z","abstract":[{"lang":"eng","text":"Differential cell adhesion and cortex tension are thought to drive cell sorting by controlling cell-cell contact formation. Here, we show that cell adhesion and cortex tension have different mechanical functions in controlling progenitor cell-cell contact formation and sorting during zebrafish gastrulation. Cortex tension controls cell-cell contact expansion by modulating interfacial tension at the contact. By contrast, adhesion has little direct function in contact expansion, but instead is needed to mechanically couple the cortices of adhering cells at their contacts, allowing cortex tension to control contact expansion. The coupling function of adhesion is mediated by E-cadherin and limited by the mechanical anchoring of E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1126/science.1225399","date_published":"2012-10-12T00:00:00Z"},{"month":"11","page":"3897 - 3904","language":[{"iso":"eng"}],"volume":139,"department":[{"_id":"CaHe"}],"publication_status":"published","quality_controlled":"1","date_created":"2018-12-11T12:00:31Z","article_processing_charge":"No","publication":"Development","author":[{"last_name":"Tada","full_name":"Tada, Masazumi","first_name":"Masazumi"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"corr_author":"1","scopus_import":"1","title":"Convergent extension Using collective cell migration and cell intercalation to shape embryos","doi":"10.1242/dev.073007","type":"journal_article","day":"01","isi":1,"acknowledgement":"M.T. is supported by the UK Medical Research Council (MRC) and Royal Society and C.-P.H. by the Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Deutsche Forschungsgemeinschaft (DFG) and Institute of Science and Technology Austria. ","citation":{"ista":"Tada M, Heisenberg C-PJ. 2012. Convergent extension Using collective cell migration and cell intercalation to shape embryos. Development. 139(21), 3897–3904.","ama":"Tada M, Heisenberg C-PJ. Convergent extension Using collective cell migration and cell intercalation to shape embryos. <i>Development</i>. 2012;139(21):3897-3904. doi:<a href=\"https://doi.org/10.1242/dev.073007\">10.1242/dev.073007</a>","ieee":"M. Tada and C.-P. J. Heisenberg, “Convergent extension Using collective cell migration and cell intercalation to shape embryos,” <i>Development</i>, vol. 139, no. 21. Company of Biologists, pp. 3897–3904, 2012.","apa":"Tada, M., &#38; Heisenberg, C.-P. J. (2012). Convergent extension Using collective cell migration and cell intercalation to shape embryos. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.073007\">https://doi.org/10.1242/dev.073007</a>","chicago":"Tada, Masazumi, and Carl-Philipp J Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” <i>Development</i>. Company of Biologists, 2012. <a href=\"https://doi.org/10.1242/dev.073007\">https://doi.org/10.1242/dev.073007</a>.","short":"M. Tada, C.-P.J. Heisenberg, Development 139 (2012) 3897–3904.","mla":"Tada, Masazumi, and Carl-Philipp J. Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” <i>Development</i>, vol. 139, no. 21, Company of Biologists, 2012, pp. 3897–904, doi:<a href=\"https://doi.org/10.1242/dev.073007\">10.1242/dev.073007</a>."},"year":"2012","publisher":"Company of Biologists","_id":"2952","status":"public","oa_version":"None","external_id":{"isi":["000309701300002"]},"issue":"21","intvolume":"       139","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Body axis elongation represents a common and fundamental morphogenetic process in development. A key mechanism triggering body axis elongation without additional growth is convergent extension (CE), whereby a tissue undergoes simultaneous narrowing and extension. Both collective cell migration and cell intercalation are thought to drive CE and are used to different degrees in various species as they elongate their body axis. Here, we provide an overview of CE as a general strategy for body axis elongation and discuss conserved and divergent mechanisms underlying CE among different species."}],"date_updated":"2025-09-30T08:10:08Z","publist_id":"3776","date_published":"2012-11-01T00:00:00Z","fulldoi":"https://doi.org/10.1242/dev.073007"},{"publist_id":"3773","date_updated":"2025-09-30T08:09:10Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1016/j.ceb.2012.09.002","date_published":"2012-10-01T00:00:00Z","oa_version":"None","_id":"2953","status":"public","publisher":"Elsevier","year":"2012","citation":{"ama":"Heisenberg C-PJ, Fässler R. Cell-cell adhesion and extracellular matrix diversity counts. <i>Current Opinion in Cell Biology</i>. 2012;24(5):559-561. doi:<a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">10.1016/j.ceb.2012.09.002</a>","ista":"Heisenberg C-PJ, Fässler R. 2012. Cell-cell adhesion and extracellular matrix diversity counts. Current Opinion in Cell Biology. 24(5), 559–561.","apa":"Heisenberg, C.-P. J., &#38; Fässler, R. (2012). Cell-cell adhesion and extracellular matrix diversity counts. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">https://doi.org/10.1016/j.ceb.2012.09.002</a>","ieee":"C.-P. J. Heisenberg and R. Fässler, “Cell-cell adhesion and extracellular matrix diversity counts,” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 5. Elsevier, pp. 559–561, 2012.","chicago":"Heisenberg, Carl-Philipp J, and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">https://doi.org/10.1016/j.ceb.2012.09.002</a>.","mla":"Heisenberg, Carl-Philipp J., and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 5, Elsevier, 2012, pp. 559–61, doi:<a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">10.1016/j.ceb.2012.09.002</a>.","short":"C.-P.J. Heisenberg, R. Fässler, Current Opinion in Cell Biology 24 (2012) 559–561."},"isi":1,"day":"01","intvolume":"        24","issue":"5","external_id":{"isi":["000310943300001"]},"scopus_import":"1","corr_author":"1","author":[{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"last_name":"Fässler","full_name":"Fässler, Reinhard","first_name":"Reinhard"}],"publication":"Current Opinion in Cell Biology","type":"journal_article","doi":"10.1016/j.ceb.2012.09.002","title":"Cell-cell adhesion and extracellular matrix diversity counts","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"CaHe"}],"volume":24,"page":"559 - 561","month":"10","article_processing_charge":"No","date_created":"2018-12-11T12:00:31Z","quality_controlled":"1"},{"day":"17","isi":1,"citation":{"chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” <i>Developmental Cell</i>. Cell Press, 2012. <a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">https://doi.org/10.1016/j.devcel.2011.12.018</a>.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” <i>Developmental Cell</i>, vol. 22, no. 1, Cell Press, 2012, pp. 3–4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">10.1016/j.devcel.2011.12.018</a>.","short":"M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 22 (2012) 3–4.","ama":"Behrndt M, Heisenberg C-PJ. Spurred by resistance mechanosensation in collective migration. <i>Developmental Cell</i>. 2012;22(1):3-4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">10.1016/j.devcel.2011.12.018</a>","ista":"Behrndt M, Heisenberg C-PJ. 2012. Spurred by resistance mechanosensation in collective migration. Developmental Cell. 22(1), 3–4.","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Spurred by resistance mechanosensation in collective migration,” <i>Developmental Cell</i>, vol. 22, no. 1. Cell Press, pp. 3–4, 2012.","apa":"Behrndt, M., &#38; Heisenberg, C.-P. J. (2012). Spurred by resistance mechanosensation in collective migration. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">https://doi.org/10.1016/j.devcel.2011.12.018</a>"},"year":"2012","publisher":"Cell Press","status":"public","_id":"3245","oa_version":"None","external_id":{"isi":["000299365800002"]},"issue":"1","intvolume":"        22","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"How cells orchestrate their behavior during collective migration is a long-standing question. Using magnetic tweezers to apply mechanical stimuli to Xenopus mesendoderm cells, Weber etal. (2012) now reveal, in this issue of Developmental Cell, a cadherin-mediated mechanosensitive response that promotes cell polarization and movement persistence during the collective mesendoderm migration in gastrulation.","lang":"eng"}],"date_updated":"2025-09-30T07:48:41Z","publist_id":"3426","date_published":"2012-01-17T00:00:00Z","fulldoi":"https://doi.org/10.1016/j.devcel.2011.12.018","month":"01","page":"3 - 4","department":[{"_id":"CaHe"}],"volume":22,"language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","date_created":"2018-12-11T12:02:14Z","article_processing_charge":"No","publication":"Developmental Cell","author":[{"first_name":"Martin","full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"corr_author":"1","scopus_import":"1","title":"Spurred by resistance mechanosensation in collective migration","doi":"10.1016/j.devcel.2011.12.018","type":"journal_article"},{"author":[{"full_name":"Barone, Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367","first_name":"Vanessa"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"publication":"Current Opinion in Cell Biology","scopus_import":"1","corr_author":"1","doi":"10.1016/j.ceb.2011.11.006","type":"journal_article","title":"Cell adhesion in embryo morphogenesis","month":"02","language":[{"iso":"eng"}],"department":[{"_id":"CaHe"}],"volume":24,"publication_status":"published","page":"148 - 153","related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"}]},"quality_controlled":"1","date_created":"2018-12-11T12:02:14Z","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Visualizing and analyzing shape changes at various scales, ranging from single molecules to whole organisms, are essential for understanding complex morphogenetic processes, such as early embryonic development. Embryo morphogenesis relies on the interplay between different tissues, the properties of which are again determined by the interaction between their constituent cells. Cell interactions, on the other hand, are controlled by various molecules, such as signaling and adhesion molecules, which in order to exert their functions need to be spatiotemporally organized within and between the interacting cells. In this review, we will focus on the role of cell adhesion functioning at different scales to organize cell, tissue and embryo morphogenesis. We will specifically ask how the subcellular distribution of adhesion molecules controls the formation of cell-cell contacts, how cell-cell contacts determine tissue shape, and how tissue interactions regulate embryo morphogenesis."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publist_id":"3423","date_updated":"2026-04-08T14:22:39Z","fulldoi":"https://doi.org/10.1016/j.ceb.2011.11.006","date_published":"2012-02-01T00:00:00Z","citation":{"ama":"Barone V, Heisenberg C-PJ. Cell adhesion in embryo morphogenesis. <i>Current Opinion in Cell Biology</i>. 2012;24(1):148-153. doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">10.1016/j.ceb.2011.11.006</a>","ista":"Barone V, Heisenberg C-PJ. 2012. Cell adhesion in embryo morphogenesis. Current Opinion in Cell Biology. 24(1), 148–153.","apa":"Barone, V., &#38; Heisenberg, C.-P. J. (2012). Cell adhesion in embryo morphogenesis. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">https://doi.org/10.1016/j.ceb.2011.11.006</a>","ieee":"V. Barone and C.-P. J. Heisenberg, “Cell adhesion in embryo morphogenesis,” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 1. Elsevier, pp. 148–153, 2012.","chicago":"Barone, Vanessa, and Carl-Philipp J Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">https://doi.org/10.1016/j.ceb.2011.11.006</a>.","short":"V. Barone, C.-P.J. Heisenberg, Current Opinion in Cell Biology 24 (2012) 148–153.","mla":"Barone, Vanessa, and Carl-Philipp J. Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 1, Elsevier, 2012, pp. 148–53, doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">10.1016/j.ceb.2011.11.006</a>."},"acknowledgement":"This review comes from a themed issue on Cell structure and dynamics Edited by Jason Swedlow and Gaudenz Danuser","year":"2012","publisher":"Elsevier","day":"01","isi":1,"oa_version":"None","_id":"3246","status":"public","intvolume":"        24","external_id":{"isi":["000302206900020"]},"issue":"1"},{"project":[{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF","grant_number":"I930-B20"}],"related_material":{"record":[{"status":"public","id":"1403","relation":"dissertation_contains"}]},"quality_controlled":"1","article_processing_charge":"No","date_created":"2018-12-11T12:00:30Z","month":"10","page":"257 - 260","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"Bio"}],"volume":338,"language":[{"iso":"eng"}],"title":"Forces driving epithelial spreading in zebrafish gastrulation","pmid":1,"type":"journal_article","doi":"10.1126/science.1224143","publication":"Science","author":[{"full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"last_name":"Campinho","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","full_name":"Campinho, Pedro","first_name":"Pedro","orcid":"0000-0002-8526-5416"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"first_name":"Felix","last_name":"Oswald","full_name":"Oswald, Felix"},{"first_name":"Julia","last_name":"Roensch","id":"4220E59C-F248-11E8-B48F-1D18A9856A87","full_name":"Roensch, Julia"},{"last_name":"Grill","full_name":"Grill, Stephan","first_name":"Stephan"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"corr_author":"1","scopus_import":"1","issue":"6104","external_id":{"pmid":["23066079"],"isi":["000309712300046"]},"intvolume":"       338","acknowledged_ssus":[{"_id":"SSU"}],"isi":1,"day":"12","publisher":"American Association for the Advancement of Science","citation":{"mla":"Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 257–60, doi:<a href=\"https://doi.org/10.1126/science.1224143\">10.1126/science.1224143</a>.","short":"M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch, S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.","chicago":"Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild, Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.1224143\">https://doi.org/10.1126/science.1224143</a>.","ieee":"M. Behrndt <i>et al.</i>, “Forces driving epithelial spreading in zebrafish gastrulation,” <i>Science</i>, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 257–260, 2012.","apa":"Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch, J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish gastrulation. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1224143\">https://doi.org/10.1126/science.1224143</a>","ama":"Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading in zebrafish gastrulation. <i>Science</i>. 2012;338(6104):257-260. doi:<a href=\"https://doi.org/10.1126/science.1224143\">10.1126/science.1224143</a>","ista":"Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation. Science. 338(6104), 257–260."},"year":"2012","acknowledgement":"We are grateful to M. Sixt, T. Bollenbach, and E. Martin-Blanco for advice and the service facilities of the IST Austria and MPI-CBG for continuous help. M.B., G.S., S.W.G., and C.-P.H. synergistically and equally developed the presented ideas and the experimental and theoretical approaches. M.B. and P.C. performed the experiments; G.S. developed the theory; and R.H., F.O., and J.R. contributed to the experimental work. This work was supported by a grant from the Fonds zur Förderung der wissenschaftlichen Forschung (FWF) and the Deutsche Forschungsgemeinschaft (DFG) (I930-B20) to C.-P.H., S.W.G., and G.S.","status":"public","_id":"2950","oa_version":"None","date_published":"2012-10-12T00:00:00Z","OA_type":"closed access","fulldoi":"https://doi.org/10.1126/science.1224143","article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Contractile actomyosin rings drive various fundamental morphogenetic processes ranging from cytokinesis to wound healing. Actomyosin rings are generally thought to function by circumferential contraction. Here, we show that the spreading of the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation is driven by a contractile actomyosin ring. In contrast to previous suggestions, we find that this ring functions not only by circumferential contraction but also by a flow-friction mechanism. This generates a pulling force through resistance against retrograde actomyosin flow. EVL spreading proceeds normally in situations where circumferential contraction is unproductive, indicating that the flow-friction mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis through a combination of cable-constriction and flow-friction mechanisms."}],"date_updated":"2026-07-29T10:07:18Z","publist_id":"3778"},{"OA_place":"publisher","title":"Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors","date_published":"2011-12-12T00:00:00Z","publication_identifier":{"issn":["2663-337X"]},"type":"dissertation","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","author":[{"first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","full_name":"Maître, Jean-Léon","last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"}],"alternative_title":["ISTA Thesis"],"publist_id":"3373","date_updated":"2026-04-09T14:36:45Z","corr_author":"1","degree_awarded":"PhD","article_processing_charge":"No","date_created":"2018-12-11T12:02:23Z","supervisor":[{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"month":"12","day":"12","publisher":"Institute of Science and Technology Austria","year":"2011","citation":{"mla":"Maître, Jean-Léon. <i>Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors</i>. Institute of Science and Technology Austria, 2011.","short":"J.-L. Maître, Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors, Institute of Science and Technology Austria, 2011.","chicago":"Maître, Jean-Léon. “Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors.” Institute of Science and Technology Austria, 2011.","apa":"Maître, J.-L. (2011). <i>Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors</i>. Institute of Science and Technology Austria.","ieee":"J.-L. Maître, “Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors,” Institute of Science and Technology Austria, 2011.","ama":"Maître J-L. Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors. 2011.","ista":"Maître J-L. 2011. Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors. Institute of Science and Technology Austria."},"_id":"3273","status":"public","oa_version":"None","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"CaHe"}]},{"status":"public","_id":"3287","oa_version":"None","day":"01","isi":1,"citation":{"chicago":"Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers, 2011. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>.","short":"V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein &#38; Peptide Science 12 (2011) 714–724.","mla":"Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8, Bentham Science Publishers, 2011, pp. 714–24, doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>.","ista":"Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single molecule trajectories? Current Protein &#38; Peptide Science. 12(8), 714–724.","ama":"Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. 2011;12(8):714-724. doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>","ieee":"V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from single molecule trajectories?,” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.","apa":"Ruprecht, V., Axmann, M., Wieser, S., &#38; Schuetz, G. (2011). What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>"},"year":"2011","publisher":"Bentham Science Publishers","external_id":{"isi":["000299672600005"]},"issue":"8","intvolume":"        12","publist_id":"3358","date_updated":"2025-09-30T09:21:31Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Diffusing membrane constituents are constantly exposed to a variety of forces that influence their stochastic path. Single molecule experiments allow for resolving trajectories at extremely high spatial and temporal accuracy, thereby offering insights into en route interactions of the tracer. In this review we discuss approaches to derive information about the underlying processes, based on single molecule tracking experiments. In particular, we focus on a new versatile way to analyze single molecule diffusion in the absence of a full analytical treatment. The method is based on comprehensive comparison of an experimental data set against the hypothetical outcome of multiple experiments performed on the computer. Since Monte Carlo simulations can be easily and rapidly performed even on state-of-the-art PCs, our method provides a simple way for testing various - even complicated - diffusion models. We describe the new method in detail, and show the applicability on two specific examples: firstly, kinetic rate constants can be derived for the transient interaction of mobile membrane proteins; secondly, residence time and corral size can be extracted for confined diffusion."}],"date_published":"2011-12-01T00:00:00Z","fulldoi":"https://doi.org/10.2174/138920311798841753","page":"714 - 724","volume":12,"department":[{"_id":"CaHe"},{"_id":"MiSi"}],"language":[{"iso":"eng"}],"publication_status":"published","month":"12","date_created":"2018-12-11T12:02:28Z","article_processing_charge":"No","quality_controlled":"1","scopus_import":"1","publication":"Current Protein & Peptide Science","author":[{"orcid":"0000-0003-4088-8633","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"full_name":"Axmann, Markus","last_name":"Axmann","first_name":"Markus"},{"last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","first_name":"Stefan"},{"first_name":"Gerhard","last_name":"Schuetz","full_name":"Schuetz, Gerhard"}],"title":"What can we learn from single molecule trajectories?","doi":"10.2174/138920311798841753","type":"journal_article"},{"_id":"3288","status":"public","oa_version":"Published Version","isi":1,"day":"22","publisher":"Public Library of Science","year":"2011","acknowledgement":"his work was funded by the National Health and Medical Research Council (NHMRC) of Australia. M.S. was an Erwin Schroedinger postdoctoral fellow of the Austrian Science Fund (FWF), S.K.W. is supported by a UQ International Research Tuition Award and Research Scholarship, S.M .by an ANZ Trustees PhD Scholarship. A.S.Y. is a Research Fellow of the NHMRC. Confocal imaging was performed at the Australian Cancer Research Foundation (ACRF) Cancer Biology Imaging Centre at the Institute for Molecular Bioscience, established with the generous support of the ACRF.","citation":{"ieee":"M. Smutny, S. Wu, G. Gomez, S. Mangold, A. Yap, and N. Hamilton, “Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens,” <i>PLoS One</i>, vol. 6, no. 7. Public Library of Science, 2011.","apa":"Smutny, M., Wu, S., Gomez, G., Mangold, S., Yap, A., &#38; Hamilton, N. (2011). Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0022458\">https://doi.org/10.1371/journal.pone.0022458</a>","ama":"Smutny M, Wu S, Gomez G, Mangold S, Yap A, Hamilton N. Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. <i>PLoS One</i>. 2011;6(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0022458\">10.1371/journal.pone.0022458</a>","ista":"Smutny M, Wu S, Gomez G, Mangold S, Yap A, Hamilton N. 2011. Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. PLoS One. 6(7).","short":"M. Smutny, S. Wu, G. Gomez, S. Mangold, A. Yap, N. Hamilton, PLoS One 6 (2011).","mla":"Smutny, Michael, et al. “Multicomponent Analysis of Junctional Movements Regulated by Myosin II Isoforms at the Epithelial Zonula Adherens.” <i>PLoS One</i>, vol. 6, no. 7, Public Library of Science, 2011, doi:<a href=\"https://doi.org/10.1371/journal.pone.0022458\">10.1371/journal.pone.0022458</a>.","chicago":"Smutny, Michael, Selwin Wu, Guillermo Gomez, Sabine Mangold, Alpha Yap, and Nicholas Hamilton. “Multicomponent Analysis of Junctional Movements Regulated by Myosin II Isoforms at the Epithelial Zonula Adherens.” <i>PLoS One</i>. Public Library of Science, 2011. <a href=\"https://doi.org/10.1371/journal.pone.0022458\">https://doi.org/10.1371/journal.pone.0022458</a>."},"issue":"7","external_id":{"isi":["000293097300049"]},"intvolume":"         6","file":[{"content_type":"application/pdf","date_updated":"2020-07-14T12:46:06Z","creator":"dernst","relation":"main_file","checksum":"57a5eb11dd05241c48c44f492b3ec3ac","access_level":"open_access","file_name":"2011_PLOS_Smutny.PDF","file_size":1984567,"date_created":"2019-05-10T10:51:43Z","file_id":"6399"}],"date_updated":"2025-09-30T09:26:01Z","publist_id":"3357","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2020-07-14T12:46:06Z","abstract":[{"lang":"eng","text":"The zonula adherens (ZA) of epithelial cells is a site of cell-cell adhesion where cellular forces are exerted and resisted. Increasing evidence indicates that E-cadherin adhesion molecules at the ZA serve to sense force applied on the junctions and coordinate cytoskeletal responses to those forces. Efforts to understand the role that cadherins play in mechanotransduction have been limited by the lack of assays to measure the impact of forces on the ZA. In this study we used 4D imaging of GFP-tagged E-cadherin to analyse the movement of the ZA. Junctions in confluent epithelial monolayers displayed prominent movements oriented orthogonal (perpendicular) to the ZA itself. Two components were identified in these movements: a relatively slow unidirectional (translational) component that could be readily fitted by least-squares regression analysis, upon which were superimposed more rapid oscillatory movements. Myosin IIB was a dominant factor responsible for driving the unilateral translational movements. In contrast, frequency spectrum analysis revealed that depletion of Myosin IIA increased the power of the oscillatory movements. This implies that Myosin IIA may serve to dampen oscillatory movements of the ZA. This extends our recent analysis of Myosin II at the ZA to demonstrate that Myosin IIA and Myosin IIB make distinct contributions to junctional movement at the ZA."}],"date_published":"2011-07-22T00:00:00Z","has_accepted_license":"1","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1371/journal.pone.0022458","publication_status":"published","language":[{"iso":"eng"}],"volume":6,"department":[{"_id":"CaHe"}],"month":"07","article_processing_charge":"No","date_created":"2018-12-11T12:02:28Z","quality_controlled":"1","publication":"PLoS One","author":[{"orcid":"0000-0002-5920-9090","first_name":"Michael","last_name":"Smutny","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael"},{"full_name":"Wu, Selwin","last_name":"Wu","first_name":"Selwin"},{"last_name":"Gomez","full_name":"Gomez, Guillermo","first_name":"Guillermo"},{"last_name":"Mangold","full_name":"Mangold, Sabine","first_name":"Sabine"},{"first_name":"Alpha","last_name":"Yap","full_name":"Yap, Alpha"},{"full_name":"Hamilton, Nicholas","last_name":"Hamilton","first_name":"Nicholas"}],"oa":1,"title":"Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens","type":"journal_article","doi":"10.1371/journal.pone.0022458"},{"publist_id":"3211","date_updated":"2025-09-30T08:42:02Z","abstract":[{"lang":"eng","text":"Recent advances in microscopy techniques and biophysical measurements have provided novel insight into the molecular, cellular and biophysical basis of cell adhesion. However, comparably little is known about a core element of cell–cell adhesion—the energy of adhesion at the cell–cell contact. In this review, we discuss approaches to understand the nature and regulation of adhesion energy, and propose strategies to determine adhesion energy between cells in vitro and in vivo."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1016/j.ceb.2011.07.004","date_published":"2011-10-01T00:00:00Z","oa_version":"Submitted Version","_id":"3397","status":"public","year":"2011","citation":{"ista":"Maître J-L, Heisenberg C-PJ. 2011. The role of adhesion energy in controlling cell-cell contacts. Current Opinion in Cell Biology. 23(5), 508–514.","ama":"Maître J-L, Heisenberg C-PJ. The role of adhesion energy in controlling cell-cell contacts. <i>Current Opinion in Cell Biology</i>. 2011;23(5):508-514. doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">10.1016/j.ceb.2011.07.004</a>","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “The role of adhesion energy in controlling cell-cell contacts,” <i>Current Opinion in Cell Biology</i>, vol. 23, no. 5. Elsevier, pp. 508–514, 2011.","apa":"Maître, J.-L., &#38; Heisenberg, C.-P. J. (2011). The role of adhesion energy in controlling cell-cell contacts. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">https://doi.org/10.1016/j.ceb.2011.07.004</a>","chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">https://doi.org/10.1016/j.ceb.2011.07.004</a>.","mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” <i>Current Opinion in Cell Biology</i>, vol. 23, no. 5, Elsevier, 2011, pp. 508–14, doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">10.1016/j.ceb.2011.07.004</a>.","short":"J.-L. Maître, C.-P.J. Heisenberg, Current Opinion in Cell Biology 23 (2011) 508–514."},"publisher":"Elsevier","day":"01","isi":1,"intvolume":"        23","external_id":{"isi":["000296040800002"]},"issue":"5","scopus_import":"1","corr_author":"1","author":[{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître","full_name":"Maître, Jean-Léon","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"publication":"Current Opinion in Cell Biology","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188705/","open_access":"1"}],"doi":"10.1016/j.ceb.2011.07.004","type":"journal_article","oa":1,"title":"The role of adhesion energy in controlling cell-cell contacts","language":[{"iso":"eng"}],"volume":23,"department":[{"_id":"CaHe"}],"publication_status":"published","page":"508 - 514","month":"10","date_created":"2018-12-11T12:03:06Z","article_processing_charge":"No","quality_controlled":"1"},{"external_id":{"isi":["000290454200007"]},"intvolume":"        95","isi":1,"day":"01","publisher":"Elsevier","citation":{"ista":"Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.","ama":"Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed. <i>Forces and Tension in Development</i>. Vol 95. Elsevier; 2011:189-213. doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>","apa":"Krens, G., &#38; Heisenberg, C.-P. J. (2011). Cell sorting in development. In M. Labouesse (Ed.), <i>Forces and Tension in Development</i> (Vol. 95, pp. 189–213). Elsevier. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>","ieee":"G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in <i>Forces and Tension in Development</i>, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp. 189–213.","chicago":"Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.” In <i>Forces and Tension in Development</i>, edited by Michel Labouesse, 95:189–213. Elsevier, 2011. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>.","short":"G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension in Development, Elsevier, 2011, pp. 189–213.","mla":"Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.” <i>Forces and Tension in Development</i>, edited by Michel Labouesse, vol. 95, Elsevier, 2011, pp. 189–213, doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>."},"year":"2011","status":"public","_id":"3791","editor":[{"first_name":"Michel","full_name":"Labouesse, Michel","last_name":"Labouesse"}],"oa_version":"None","date_published":"2011-01-01T00:00:00Z","fulldoi":"https://doi.org/10.1016/B978-0-12-385065-2.00006-2","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active maintenance of their segregated state. Because of its biological importance and apparent resemblance to fluid segregation in physics, cell sorting was extensively studied by both biologists and physicists over the last decades. Different theories were developed that try to explain cell sorting on the basis of the physical properties of the constituent cells. However, only recently the molecular and cellular mechanisms that control the physical properties driving cell sorting, have begun to be unraveled. In this review, we will provide an overview of different cell-sorting processes in development and discuss how these processes can be explained by the different sorting theories, and how these theories in turn can be connected to the molecular and cellular mechanisms driving these processes."}],"date_updated":"2025-09-30T08:37:44Z","alternative_title":["Current Topics in Developmental Biology"],"publist_id":"2436","quality_controlled":"1","article_processing_charge":"No","date_created":"2018-12-11T12:05:11Z","month":"01","page":"189 - 213","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"CaHe"}],"volume":95,"title":"Cell sorting in development","type":"book_chapter","doi":"10.1016/B978-0-12-385065-2.00006-2","publication":"Forces and Tension in Development","author":[{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","full_name":"Krens, Gabriel"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"corr_author":"1","scopus_import":"1"},{"scopus_import":"1","corr_author":"1","author":[{"first_name":"Petra","full_name":"Stockinger, Petra","last_name":"Stockinger","id":"261CB030-E90D-11E9-B182-F697D44B663C"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"},{"full_name":"Maître, Jean-Léon","last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474","first_name":"Jean-Léon"}],"publication":"Development","doi":"10.1242/dev.071233","type":"journal_article","oa":1,"title":"Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":138,"publication_status":"published","page":"4673 - 4683","month":"11","date_created":"2018-12-11T12:03:06Z","article_processing_charge":"No","quality_controlled":"1","publist_id":"3210","date_updated":"2026-07-28T08:23:30Z","file_date_updated":"2020-07-14T12:46:12Z","abstract":[{"text":"Facial branchiomotor neurons (FBMNs) in zebrafish and mouse embryonic hindbrain undergo a characteristic tangential migration from rhombomere (r) 4, where they are born, to r6/7. Cohesion among neuroepithelial cells (NCs) has been suggested to function in FBMN migration by inhibiting FBMNs positioned in the basal neuroepithelium such that they move apically between NCs towards the midline of the neuroepithelium instead of tangentially along the basal side of the neuroepithelium towards r6/7. However, direct experimental evaluation of this hypothesis is still lacking. Here, we have used a combination of biophysical cell adhesion measurements and high-resolution time-lapse microscopy to determine the role of NC cohesion in FBMN migration. We show that reducing NC cohesion by interfering with Cadherin 2 (Cdh2) activity results in FBMNs positioned at the basal side of the neuroepithelium moving apically towards the neural tube midline instead of tangentially towards r6/7. In embryos with strongly reduced NC cohesion, ectopic apical FBMN movement frequently results in fusion of the bilateral FBMN clusters over the apical midline of the neural tube. By contrast, reducing cohesion among FBMNs by interfering with Contactin 2 (Cntn2) expression in these cells has little effect on apical FBMN movement, but reduces the fusion of the bilateral FBMN clusters in embryos with strongly diminished NC cohesion. These data provide direct experimental evidence that NC cohesion functions in tangential FBMN migration by restricting their apical movement.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","fulldoi":"https://doi.org/10.1242/dev.071233","keyword":["Epithelial cohesion","Hindbrain","Neuronal migration","Zebrafish"],"ddc":["570"],"has_accepted_license":"1","date_published":"2011-11-01T00:00:00Z","oa_version":"Published Version","_id":"3396","status":"public","citation":{"apa":"Stockinger, P., Heisenberg, C.-P. J., &#38; Maître, J.-L. (2011). Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>","ieee":"P. Stockinger, C.-P. J. Heisenberg, and J.-L. Maître, “Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube,” <i>Development</i>, vol. 138, no. 21. Company of Biologists, pp. 4673–4683, 2011.","ama":"Stockinger P, Heisenberg C-PJ, Maître J-L. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. 2011;138(21):4673-4683. doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>","ista":"Stockinger P, Heisenberg C-PJ, Maître J-L. 2011. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. 138(21), 4673–4683.","short":"P. Stockinger, C.-P.J. Heisenberg, J.-L. Maître, Development 138 (2011) 4673–4683.","mla":"Stockinger, Petra, et al. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>, vol. 138, no. 21, Company of Biologists, 2011, pp. 4673–83, doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>.","chicago":"Stockinger, Petra, Carl-Philipp J Heisenberg, and Jean-Léon Maître. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>. Company of Biologists, 2011. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>."},"acknowledgement":"We thank C. Moens and J. Geiger for critical reading of earlier versions of this manuscript and members of the Heisenberg laboratory for discussions. We are grateful to the microscopy facility of the MPI-CBG and IST Austria for continuous support; I. Nüsslein, J. Compagnon and Alex Eichner for help with cell sorting; and the fish facility of the MPI-CBG and IST Austria for excellent fish care.","year":"2011","publisher":"Company of Biologists","day":"01","isi":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"file":[{"date_updated":"2020-07-14T12:46:12Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","checksum":"ca12b79e01ef36c1ef1aea31cf7e7139","access_level":"open_access","file_name":"2011_Development_Stockinger.pdf","date_created":"2019-10-07T14:19:42Z","file_size":4672439,"file_id":"6930"}],"intvolume":"       138","external_id":{"isi":["000296060100011"]},"issue":"21"},{"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090540/","open_access":"1"}],"doi":"10.1016/j.ydbio.2011.03.025","type":"journal_article","pmid":1,"oa":1,"title":"Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail","scopus_import":"1","author":[{"last_name":"Row","full_name":"Row, Richard","first_name":"Richard"},{"first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître","full_name":"Maître, Jean-Léon"},{"first_name":"Benjamin","full_name":"Martin, Benjamin","last_name":"Martin"},{"last_name":"Stockinger","id":"261CB030-E90D-11E9-B182-F697D44B663C","full_name":"Stockinger, Petra","first_name":"Petra"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"},{"first_name":"David","last_name":"Kimelman","full_name":"Kimelman, David"}],"publication":"Developmental Biology","date_created":"2018-12-11T12:03:00Z","article_processing_charge":"No","quality_controlled":"1","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":354,"publication_status":"published","page":"102 - 110","month":"06","fulldoi":"https://doi.org/10.1016/j.ydbio.2011.03.025","article_type":"original","OA_type":"green","date_published":"2011-06-01T00:00:00Z","OA_place":"repository","date_updated":"2026-07-28T08:31:27Z","publist_id":"3228","abstract":[{"lang":"eng","text":"The process of gastrulation is highly conserved across vertebrates on both the genetic and morphological levels, despite great variety in embryonic shape and speed of development. This mechanism spatially separates the germ layers and establishes the organizational foundation for future development. Mesodermal identity is specified in a superficial layer of cells, the epiblast, where cells maintain an epithelioid morphology. These cells involute to join the deeper hypoblast layer where they adopt a migratory, mesenchymal morphology. Expression of a cascade of related transcription factors orchestrates the parallel genetic transition from primitive to mature mesoderm. Although the early and late stages of this process are increasingly well understood, the transition between them has remained largely mysterious. We present here the first high resolution in vivo observations of the blebby transitional morphology of involuting mesodermal cells in a vertebrate embryo. We further demonstrate that the zebrafish spadetail mutation creates a reversible block in the maturation program, stalling cells in the transition state. This mutation creates an ideal system for dissecting the specific properties of cells undergoing the morphological transition of maturing mesoderm, as we demonstrate with a direct measurement of cell–cell adhesion."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"       354","external_id":{"isi":["000290550500010"],"pmid":["21463614"]},"issue":"1","oa_version":"Submitted Version","_id":"3379","status":"public","year":"2011","citation":{"ista":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. 2011. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology. 354(1), 102–110.","ama":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. <i>Developmental Biology</i>. 2011;354(1):102-110. doi:<a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">10.1016/j.ydbio.2011.03.025</a>","apa":"Row, R., Maître, J.-L., Martin, B., Stockinger, P., Heisenberg, C.-P. J., &#38; Kimelman, D. (2011). Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. <i>Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">https://doi.org/10.1016/j.ydbio.2011.03.025</a>","ieee":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P. J. Heisenberg, and D. Kimelman, “Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail,” <i>Developmental Biology</i>, vol. 354, no. 1. Elsevier, pp. 102–110, 2011.","chicago":"Row, Richard, Jean-Léon Maître, Benjamin Martin, Petra Stockinger, Carl-Philipp J Heisenberg, and David Kimelman. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">https://doi.org/10.1016/j.ydbio.2011.03.025</a>.","short":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P.J. Heisenberg, D. Kimelman, Developmental Biology 354 (2011) 102–110.","mla":"Row, Richard, et al. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>, vol. 354, no. 1, Elsevier, 2011, pp. 102–10, doi:<a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">10.1016/j.ydbio.2011.03.025</a>."},"acknowledgement":"We thank David Grunwald for providing the spadetail-myc fusion construct. This work was supported by an NIH grant (GM079203) to D.K. and grants from the Austrian Academy of Sciences to P.S., and from the DFG, MPG and IST Austria to C.-P.H. R.R. was supported by a Developmental Biology Predoctoral Training Grant, T32HD007183, from the National Institute of Child Health and Human Development. B.L.M. was supported by an American Cancer Society fellowship (PF-07-048-01-DDC).","publisher":"Elsevier","das_tickbox":"1","day":"01","isi":1},{"month":"07","page":"24 - 24","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":278,"publication_status":"published","quality_controlled":"1","date_created":"2018-12-11T12:03:01Z","article_processing_charge":"No","publication":"The FEBS Journal","author":[{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"corr_author":"1","conference":{"start_date":"2011-06-25","location":"Torino, Italy","end_date":"2011-06-30","name":"FEBS Congress on Biochemistry for Tomorrow's Medicine"},"title":"Invited Lectures ‐ Symposia Area","oa":1,"main_file_link":[{"url":"https://doi.org/10.1111/j.1742-4658.2011.08136.x","open_access":"1"}],"doi":"10.1111/j.1742-4658.2011.08136.x","type":"conference_abstract","day":"01","citation":{"short":"C.-P.J. Heisenberg, in:, The FEBS Journal, Wiley, 2011, pp. 24–24.","mla":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” <i>The FEBS Journal</i>, vol. 278, no. S1, Wiley, 2011, pp. 24–24, doi:<a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">10.1111/j.1742-4658.2011.08136.x</a>.","chicago":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” In <i>The FEBS Journal</i>, 278:24–24. Wiley, 2011. <a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">https://doi.org/10.1111/j.1742-4658.2011.08136.x</a>.","apa":"Heisenberg, C.-P. J. (2011). Invited Lectures ‐ Symposia Area. In <i>The FEBS Journal</i> (Vol. 278, pp. 24–24). Torino, Italy: Wiley. <a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">https://doi.org/10.1111/j.1742-4658.2011.08136.x</a>","ieee":"C.-P. J. Heisenberg, “Invited Lectures ‐ Symposia Area,” in <i>The FEBS Journal</i>, Torino, Italy, 2011, vol. 278, no. S1, pp. 24–24.","ama":"Heisenberg C-PJ. Invited Lectures ‐ Symposia Area. In: <i>The FEBS Journal</i>. Vol 278. Wiley; 2011:24-24. doi:<a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">10.1111/j.1742-4658.2011.08136.x</a>","ista":"Heisenberg C-PJ. 2011. Invited Lectures ‐ Symposia Area. The FEBS Journal. FEBS Congress on Biochemistry for Tomorrow’s Medicine vol. 278, 24–24."},"year":"2011","publisher":"Wiley","_id":"3383","status":"public","oa_version":"Published Version","issue":"S1","intvolume":"       278","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-07-28T08:27:55Z","publist_id":"3224","OA_place":"publisher","date_published":"2011-07-01T00:00:00Z","OA_type":"free access","fulldoi":"https://doi.org/10.1111/j.1742-4658.2011.08136.x"},{"intvolume":"       108","external_id":{"pmid":["21212360"],"isi":["000286310300003"]},"issue":"3","oa_version":"Submitted Version","status":"public","_id":"3368","citation":{"chicago":"Krens, Gabriel, Stephanie Möllmert, and Carl-Philipp J Heisenberg. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>. National Academy of Sciences, 2011. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>.","short":"G. Krens, S. Möllmert, C.-P.J. Heisenberg, PNAS 108 (2011) E9–E10.","mla":"Krens, Gabriel, et al. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>, vol. 108, no. 3, National Academy of Sciences, 2011, pp. E9–10, doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>.","ama":"Krens G, Möllmert S, Heisenberg C-PJ. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. 2011;108(3):E9-E10. doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>","ista":"Krens G, Möllmert S, Heisenberg C-PJ. 2011. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. 108(3), E9–E10.","apa":"Krens, G., Möllmert, S., &#38; Heisenberg, C.-P. J. (2011). Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>","ieee":"G. Krens, S. Möllmert, and C.-P. J. Heisenberg, “Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants,” <i>PNAS</i>, vol. 108, no. 3. National Academy of Sciences, pp. E9–E10, 2011."},"year":"2011","publisher":"National Academy of Sciences","das_tickbox":"1","day":"18","isi":1,"article_type":"letter_note","fulldoi":"https://doi.org/10.1073/pnas.1010767108","date_published":"2011-01-18T00:00:00Z","date_updated":"2026-07-28T09:03:18Z","publist_id":"3244","abstract":[{"lang":"eng","text":"Tissue surface tension (TST) is an important mechanical property influencing cell sorting and tissue envelopment. The study by Manning et al. (1) reported on a mathematical model describing TST on the basis of the balance between adhesive and tensile properties of the constituent cells. The model predicts that, in high-adhesion cell aggregates, surface cells will be stretched to maintain the same area of cell–cell contact as interior bulk cells, resulting in an elongated and flattened cell shape. The authors (1) observed flat and elongated cells at the surface of high-adhesion zebrafish germ-layer explants, which they argue are undifferentiated stretched germ-layer progenitor cells, and they use this observation as a validation of their model."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T12:02:56Z","article_processing_charge":"No","quality_controlled":"1","department":[{"_id":"CaHe"}],"volume":108,"language":[{"iso":"eng"}],"publication_status":"published","page":"E9 - E10","month":"01","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024655","open_access":"1"}],"doi":"10.1073/pnas.1010767108","type":"journal_article","pmid":1,"oa":1,"title":"Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants","scopus_import":"1","corr_author":"1","author":[{"first_name":"Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","full_name":"Krens, Gabriel"},{"first_name":"Stephanie","id":"260FD49C-E911-11E9-B5EA-D9538404589B","last_name":"Möllmert","full_name":"Möllmert, Stephanie"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publication":"PNAS"}]