---
_id: '3790'
abstract:
- lang: eng
  text: Cell shape and motility are primarily controlled by cellular mechanics. The
    attachment of the plasma membrane to the underlying actomyosin cortex has been
    proposed to be important for cellular processes involving membrane deformation.
    However, little is known about the actual function of membrane-to-cortex attachment
    (MCA) in cell protrusion formation and migration, in particular in the context
    of the developing embryo. Here, we use a multidisciplinary approach to study MCA
    in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells,
    which migrate using a combination of different protrusion types, namely, lamellipodia,
    filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity
    of molecules linking the cortex to the membrane and measuring resulting changes
    in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors
    increases the proportion of cellular blebs and reduces the directionality of cell
    migration. We propose that MCA is a key parameter controlling the relative proportions
    of different cell protrusion types in mesendoderm progenitors, and thus is key
    in controlling directed migration during gastrulation.
acknowledgement: "We would like to thank A. G. Clark, S. Grill, A. Oates, E. Raz,
  L. Rohde, and M. Zerial for reading earlier versions of the manuscript. We are grateful
  to W. Zachariae, Y. Arboleda-Estudillo, S. Schneider, P. Stockinger, D. Panhans,
  M. Biro, J. C. Olaya, and the BIOTEC/MPI-CBG zebrafish and imaging facilities for
  help and advice at various stages of this project and to J. Helenius for help with
  programming. This work was supported by grants from the Boehringer Ingelheim Fonds
  to MK, the Polish Ministry of Science and Higher Education to E. P., and the Deutsche
  Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EP.\r\n"
article_number: e1000544
article_processing_charge: No
author:
- first_name: Alba
  full_name: Diz Muñoz, Alba
  last_name: Diz Muñoz
- first_name: Michael
  full_name: Krieg, Michael
  last_name: Krieg
- first_name: Martin
  full_name: Bergert, Martin
  last_name: Bergert
- first_name: Itziar
  full_name: Ibarlucea Benitez, Itziar
  last_name: Ibarlucea Benitez
- first_name: Daniel
  full_name: Müller, Daniel
  last_name: Müller
- first_name: Ewa
  full_name: Paluch, Ewa
  last_name: Paluch
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Diz Muñoz A, Krieg M, Bergert M, et al. Control of directed cell migration
    in vivo by membrane-to-cortex attachment. <i>PLoS Biology</i>. 2010;8(11). doi:<a
    href="https://doi.org/10.1371/journal.pbio.1000544">10.1371/journal.pbio.1000544</a>
  apa: Diz Muñoz, A., Krieg, M., Bergert, M., Ibarlucea Benitez, I., Müller, D., Paluch,
    E., &#38; Heisenberg, C.-P. J. (2010). Control of directed cell migration in vivo
    by membrane-to-cortex attachment. <i>PLoS Biology</i>. Public Library of Science.
    <a href="https://doi.org/10.1371/journal.pbio.1000544">https://doi.org/10.1371/journal.pbio.1000544</a>
  chicago: Diz Muñoz, Alba, Michael Krieg, Martin Bergert, Itziar Ibarlucea Benitez,
    Daniel Müller, Ewa Paluch, and Carl-Philipp J Heisenberg. “Control of Directed
    Cell Migration in Vivo by Membrane-to-Cortex Attachment.” <i>PLoS Biology</i>.
    Public Library of Science, 2010. <a href="https://doi.org/10.1371/journal.pbio.1000544">https://doi.org/10.1371/journal.pbio.1000544</a>.
  ieee: A. Diz Muñoz <i>et al.</i>, “Control of directed cell migration in vivo by
    membrane-to-cortex attachment,” <i>PLoS Biology</i>, vol. 8, no. 11. Public Library
    of Science, 2010.
  ista: Diz Muñoz A, Krieg M, Bergert M, Ibarlucea Benitez I, Müller D, Paluch E,
    Heisenberg C-PJ. 2010. Control of directed cell migration in vivo by membrane-to-cortex
    attachment. PLoS Biology. 8(11), e1000544.
  mla: Diz Muñoz, Alba, et al. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex
    Attachment.” <i>PLoS Biology</i>, vol. 8, no. 11, e1000544, Public Library of
    Science, 2010, doi:<a href="https://doi.org/10.1371/journal.pbio.1000544">10.1371/journal.pbio.1000544</a>.
  short: A. Diz Muñoz, M. Krieg, M. Bergert, I. Ibarlucea Benitez, D. Müller, E. Paluch,
    C.-P.J. Heisenberg, PLoS Biology 8 (2010).
date_created: 2018-12-11T12:05:11Z
date_published: 2010-11-30T00:00:00Z
date_updated: 2025-09-30T09:39:58Z
day: '30'
ddc:
- '576'
department:
- _id: CaHe
doi: 10.1371/journal.pbio.1000544
external_id:
  isi:
  - '000284762300016'
file:
- access_level: open_access
  checksum: 52d18c90ca6b02234cea5e8b399b7f46
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:08:24Z
  date_updated: 2020-07-14T12:46:16Z
  file_id: '4685'
  file_name: IST-2015-365-v1+1_journal.pbio.1000544.pdf
  file_size: 799506
  relation: main_file
file_date_updated: 2020-07-14T12:46:16Z
has_accepted_license: '1'
intvolume: '         8'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: PLoS Biology
publication_status: published
publisher: Public Library of Science
publist_id: '2437'
pubrep_id: '365'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Control of directed cell migration in vivo by membrane-to-cortex attachment
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 8
year: '2010'
...
---
OA_place: publisher
_id: '3962'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Holger
  full_name: Pflicke, Holger
  id: CAA57A9A-5B61-11E9-B130-E0C1E1F2C83D
  last_name: Pflicke
citation:
  ama: Pflicke H.   Dendritic cell migration across basement membranes in the skin.
    2010.
  apa: Pflicke, H. (2010). <i>  Dendritic cell migration across basement membranes
    in the skin</i>. Institute of Science and Technology Austria.
  chicago: Pflicke, Holger. “  Dendritic Cell Migration across Basement Membranes
    in the Skin.” Institute of Science and Technology Austria, 2010.
  ieee: H. Pflicke, “  Dendritic cell migration across basement membranes in the skin,”
    Institute of Science and Technology Austria, 2010.
  ista: Pflicke H. 2010.   Dendritic cell migration across basement membranes in the
    skin. Institute of Science and Technology Austria.
  mla: Pflicke, Holger. <i>  Dendritic Cell Migration across Basement Membranes in
    the Skin</i>. Institute of Science and Technology Austria, 2010.
  short: H. Pflicke,   Dendritic Cell Migration across Basement Membranes in the Skin,
    Institute of Science and Technology Austria, 2010.
corr_author: '1'
date_created: 2018-12-11T12:06:08Z
date_published: 2010-07-01T00:00:00Z
date_updated: 2026-04-09T14:37:07Z
day: '01'
degree_awarded: PhD
department:
- _id: CaHe
- _id: GradSch
language:
- iso: eng
month: '07'
oa_version: None
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '2165'
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: "\uFEFF\uFEFFDendritic cell migration across basement membranes in the skin"
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2010'
...
---
_id: '4157'
abstract:
- lang: eng
  text: Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis,
    allowing cells and tissues to change shape without loosing integrity. Studies
    predominantly in cell culture showed that mechanosensation through adhesion structures
    is achieved by force-mediated modulation of their molecular composition. The specific
    molecular composition of adhesion sites in turn determines their signalling activity
    and dynamic reorganization. Here, we will review how adhesion sites respond to
    mecanical stimuli, and how spatially and temporally regulated signalling from
    different adhesion sites controls cell migration and tissue morphogenesis.
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent
    regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. 2010;29(16):2753-2768.
    doi:<a href="https://doi.org/10.1038/emboj.2010.182">10.1038/emboj.2010.182</a>'
  apa: 'Papusheva, E., &#38; Heisenberg, C.-P. J. (2010). Spatial organization of
    adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO
    Journal</i>. Wiley-Blackwell. <a href="https://doi.org/10.1038/emboj.2010.182">https://doi.org/10.1038/emboj.2010.182</a>'
  chicago: 'Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization
    of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
    <i>EMBO Journal</i>. Wiley-Blackwell, 2010. <a href="https://doi.org/10.1038/emboj.2010.182">https://doi.org/10.1038/emboj.2010.182</a>.'
  ieee: 'E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion:
    force-dependent regulation and function in tissue morphogenesis,” <i>EMBO Journal</i>,
    vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010.'
  ista: 'Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent
    regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.'
  mla: 'Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization
    of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
    <i>EMBO Journal</i>, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:<a
    href="https://doi.org/10.1038/emboj.2010.182">10.1038/emboj.2010.182</a>.'
  short: E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.
date_created: 2018-12-11T12:07:17Z
date_published: 2010-08-18T00:00:00Z
date_updated: 2025-09-30T09:29:30Z
day: '18'
department:
- _id: Bio
- _id: CaHe
doi: 10.1038/emboj.2010.182
external_id:
  isi:
  - '000281006400009'
  pmid:
  - '20717145'
intvolume: '        29'
isi: 1
issue: '16'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/
month: '08'
oa: 1
oa_version: Submitted Version
page: 2753 - 2768
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1962'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Spatial organization of adhesion: force-dependent regulation and function
  in tissue morphogenesis'
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 29
year: '2010'
...