--- OA_place: publisher _id: '20575' abstract: - lang: eng text: "This thesis deals with eigenvalue and eigenvector universality results for random matrix ensembles equipped with non-trivial spatial structure. We consider both mean-field models with a general variance profile (Wigner-type matrices) and correlation structure (correlated matrices) among the entries, as well as non-mean-field random band matrices with bandwidth W >> N^(1/2).\r\n\r\nTo extract the universal properties of random matrix spectra and eigenvectors, we obtain concentration estimates for their resolvent, the local laws, which generalize the celebrated Wigner semicircle law for a broad class of random matrices to much finer spectral scales. The local laws hold for both a single resolvent as well as for products of multiple resolvents, known as resolvent chains, and express the remarkable approximately-deterministic behavior of these objects down to the microscopic scale.\r\n\r\nOur primary tool for establishing the local laws is the dynamical Zigzag strategy, which we develop in the setting of spatially-inhomogeneous random matrices. Our proof method systematically addresses the challenges arising from non-trivial spatial structures and is robust to all types of singularities in the spectrum, as we demonstrate in the correlated setting. Furthermore, we incorporate the analysis of the deterministic resolvent chain approximations into the dynamical framework of the Zigzag strategy, synthesizing a unified toolkit for establishing multi-resolvent local laws.\r\n\r\nUsing these methods, we prove complete eigenvector delocalization, the Eigenstate Thermalization Hypothesis, and Wigner-Dyson universality in the bulk for random band matrices down to the optimal bandwidth W >> N^(1/2). For mean-field ensembles, we establish universality of local eigenvalue statistics at the cups for random matrices with correlated entries, and the Eigenstate Thermalization Hypothesis for Wigner-type matrices in the bulk of the spectrum.\r\n\r\nFinally, this thesis also contains other applications of the multi-resolvent local laws to spatially-inhomogeneous random matrices, obtained prior to the development of the Zigzag strategy. In particular, we provide a complete analysis of mesoscopic linear-eigenvalue statistics of Wigner-type matrices in all spectral regimes, including the novel cusps, and rigorously establish the prethermalization phenomenon for deformed Wigner matrices.\r\n\r\nThe main body of this thesis consists of seven research papers (listed on page xi), each presented in a separate chapter with its own introduction and all relevant context, suitable to be read independently. We ask the reader’s indulgence for the repetitions in the historical overviews and other minor redundancies that remain among the chapters as a result. The overall Introduction, preceding the chapters, provides a condensed, informal summary of the main ideas and concepts at the core of these works.\r\n" acknowledgement: "The work comprising this thesis was supported by the ERC Advanced Grant \"RMTBeyond\"\r\nNo.101020331 awarded to my advisor." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Volodymyr full_name: Riabov, Volodymyr id: 1949f904-edfb-11eb-afb5-e2dfddabb93b last_name: Riabov citation: ama: Riabov V. Universality in random matrices with spatial structure. 2025. doi:10.15479/AT-ISTA-20575 apa: Riabov, V. (2025). Universality in random matrices with spatial structure. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20575 chicago: Riabov, Volodymyr. “Universality in Random Matrices with Spatial Structure.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20575. ieee: V. Riabov, “Universality in random matrices with spatial structure,” Institute of Science and Technology Austria, 2025. ista: Riabov V. 2025. Universality in random matrices with spatial structure. Institute of Science and Technology Austria. mla: Riabov, Volodymyr. Universality in Random Matrices with Spatial Structure. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20575. short: V. Riabov, Universality in Random Matrices with Spatial Structure, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-29T19:12:24Z date_published: 2025-11-03T00:00:00Z date_updated: 2026-10-16T08:41:47Z day: '3' ddc: - '515' - '519' degree_awarded: PhD department: - _id: GradSch - _id: LaEr doi: 10.15479/AT-ISTA-20575 ec_funded: 1 file: - access_level: open_access checksum: 6a0487b2b66bb35d44b394756d44b8b4 content_type: application/pdf creator: vriabov date_created: 2025-10-29T18:53:59Z date_updated: 2025-10-29T18:53:59Z file_id: '20577' file_name: riabov_thesis-pdfa.pdf file_size: 7536583 relation: main_file success: 1 - access_level: closed checksum: 224efda6bf9864d296a1e5e0124c1e8f content_type: application/x-zip-compressed creator: vriabov date_created: 2025-10-29T18:54:53Z date_updated: 2025-10-29T18:54:53Z file_id: '20578' file_name: manuscript.zip file_size: 17841612 relation: source_file file_date_updated: 2025-10-29T18:54:53Z fulldoi: https://doi.org/10.15479/AT-ISTA-20575 has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '11' oa: 1 oa_version: Published Version page: '436' project: - _id: 62796744-2b32-11ec-9570-940b20777f1d call_identifier: H2020 grant_number: '101020331' name: Random matrices beyond Wigner-Dyson-Mehta publication_identifier: isbn: - 978-3-99078-064-0 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20322' relation: part_of_dissertation status: public - id: '13317' relation: part_of_dissertation status: public - id: '19368' relation: part_of_dissertation status: deleted - id: '20576' relation: part_of_dissertation status: public - id: '17174' relation: part_of_dissertation status: public - id: '19547' relation: part_of_dissertation status: public - id: '19598' relation: part_of_dissertation status: public - id: '18554' relation: part_of_dissertation status: public - id: '18764' relation: part_of_dissertation status: public status: public supervisor: - first_name: László full_name: Erdös, László id: 4DBD5372-F248-11E8-B48F-1D18A9856A87 last_name: Erdös orcid: 0000-0001-5366-9603 title: Universality in random matrices with spatial structure tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19540' abstract: - lang: eng text: "This thesis deals with several different models for complex quantum mechanical systems and is structured in three main parts. \r\n\t\r\nIn Part I, we study mean field random matrices as models for quantum Hamiltonians. Our focus lies on proving concentration estimates for resolvents of random matrices, so-called local laws, mostly in the setting of multiple resolvents. These estimates have profound consequences for eigenvector overlaps and thermalization problems. More concretely, we obtain, e.g., the optimal eigenstate thermalization hypothesis (ETH) uniformly in the spectrum for Wigner matrices, an optimal lower bound on non-Hermitian eigenvector overlaps, and prethermalization for deformed Wigner matrices.\tIn order to prove our novel multi-resolvent local laws, we develop and devise two main methods, the static Psi-method and the dynamical Zigzag strategy. \r\n\t\r\nIn Part II, we study Bardeen-Cooper-Schrieffer (BCS) theory, the standard mean field microscopic theory of superconductivity. We focus on asymptotic formulas for the characteristic critical temperature and energy gap of a superconductor and prove universality of their ratio in various physical regimes. Additionally, we investigate multi-band superconductors and show that inter-band coupling effects can only enhance the critical temperature. \r\n\t\r\nIn Part III, we study quantum lattice systems. On the one hand, we show a strong version of the local-perturbations-perturb-locally (LPPL) principle for the ground state of weakly interacting quantum spin systems with a uniform on-site gap. On the other hand, we introduce a notion of a local gap and rigorously justify response theory and the Kubo formula under the weakened assumption of a local gap. \r\n\t\r\nAdditionally, we discuss two classes of problems which do not fit into the three main parts of the thesis. These are deformational rigidity of Liouville metrics on the torus and relativistic toy models of particle creation via interior-boundary-conditions (IBCs). " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Sven Joscha full_name: Henheik, Sven Joscha id: 31d731d7-d235-11ea-ad11-b50331c8d7fb last_name: Henheik orcid: 0000-0003-1106-327X citation: ama: 'Henheik SJ. Modeling complex quantum systems: Random matrices, BCS theory, and quantum lattice systems. 2025. doi:10.15479/AT-ISTA-19540' apa: 'Henheik, S. J. (2025). Modeling complex quantum systems: Random matrices, BCS theory, and quantum lattice systems. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19540' chicago: 'Henheik, Sven Joscha. “Modeling Complex Quantum Systems: Random Matrices, BCS Theory, and Quantum Lattice Systems.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19540.' ieee: 'S. J. Henheik, “Modeling complex quantum systems: Random matrices, BCS theory, and quantum lattice systems,” Institute of Science and Technology Austria, 2025.' ista: 'Henheik SJ. 2025. Modeling complex quantum systems: Random matrices, BCS theory, and quantum lattice systems. Institute of Science and Technology Austria.' mla: 'Henheik, Sven Joscha. Modeling Complex Quantum Systems: Random Matrices, BCS Theory, and Quantum Lattice Systems. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19540.' short: 'S.J. Henheik, Modeling Complex Quantum Systems: Random Matrices, BCS Theory, and Quantum Lattice Systems, Institute of Science and Technology Austria, 2025.' corr_author: '1' date_created: 2025-04-10T21:21:18Z date_published: 2025-04-10T00:00:00Z date_updated: 2026-10-16T08:41:47Z day: '10' ddc: - '519' degree_awarded: PhD department: - _id: GradSch - _id: LaEr doi: 10.15479/AT-ISTA-19540 doi_confirm: '1' ec_funded: 1 file: - access_level: closed checksum: b8477ae5578436c72c3bb4193ad34ac5 content_type: application/zip creator: shenheik date_created: 2025-04-10T21:14:18Z date_updated: 2025-04-10T21:14:18Z file_id: '19542' file_name: Henheik-Thesis_source_final.zip file_size: 4107587 relation: source_file - access_level: open_access checksum: e9fc0ea12ec46c9f71110c33217c4140 content_type: application/pdf creator: shenheik date_created: 2025-04-11T13:16:05Z date_updated: 2025-04-11T13:16:05Z file_id: '19553' file_name: Henheik-Thesis-pdfa_FINAL.pdf file_size: 9999492 relation: main_file success: 1 - access_level: closed checksum: f94580f86c785e7108eb116cd189e225 content_type: application/pdf creator: cchlebak date_created: 2025-04-23T14:10:27Z date_updated: 2025-04-23T14:10:27Z file_id: '19615' file_name: Henheik-Thesis-Volume1_print.pdf file_size: 13276442 relation: other - access_level: closed checksum: b927ead3c78020ffb32918911deedb74 content_type: application/pdf creator: cchlebak date_created: 2025-04-23T14:11:05Z date_updated: 2025-04-23T14:11:05Z file_id: '19616' file_name: Henheik-Thesis-Volume2_print.pdf file_size: 7628767 relation: other file_date_updated: 2025-04-23T14:11:05Z fulldoi: https://doi.org/10.15479/AT-ISTA-19540 has_accepted_license: '1' language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: '720' project: - _id: 62796744-2b32-11ec-9570-940b20777f1d call_identifier: H2020 grant_number: '101020331' name: Random matrices beyond Wigner-Dyson-Mehta publication_identifier: isbn: - 978-3-99078-057-2 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '14343' relation: part_of_dissertation status: public - id: '13317' relation: part_of_dissertation status: public - id: '11732' relation: part_of_dissertation status: public - id: '12184' relation: part_of_dissertation status: public - id: '14421' relation: part_of_dissertation status: public - id: '10623' relation: part_of_dissertation status: public - id: '18112' relation: part_of_dissertation status: public - id: '19001' relation: part_of_dissertation status: public - id: '10642' relation: part_of_dissertation status: public - id: '19545' relation: part_of_dissertation status: public - id: '19546' relation: part_of_dissertation status: public - id: '19550' relation: part_of_dissertation status: public - id: '19551' relation: part_of_dissertation status: public - id: '19552' relation: part_of_dissertation status: public - id: '19547' relation: part_of_dissertation status: public - id: '19548' relation: part_of_dissertation status: public - id: '14542' relation: part_of_dissertation status: public - id: '18656' relation: part_of_dissertation status: public - id: '17049' relation: part_of_dissertation status: public - id: '18764' relation: part_of_dissertation status: public status: public supervisor: - first_name: László full_name: Erdös, László id: 4DBD5372-F248-11E8-B48F-1D18A9856A87 last_name: Erdös orcid: 0000-0001-5366-9603 title: 'Modeling complex quantum systems: Random matrices, BCS theory, and quantum lattice systems' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher _id: '20741' abstract: - lang: eng text: "Life on Earth emerged when biomacromolecules were membrane-enclosed in a confined space where many essential chemical reactions were more likely to happen and thereby accelerate evolution. These kinds of membranes separated internal reactions from the outside chaos while staying flexible so that those primordial cells can move, adopt their shape and, most importantly, propagate. Such membrane plasticity still remains a defining feature of all modern cell types. This remarkable ability to change their shape is most prominently observed during their propagation (i.e., cell division). Throughout division, a cell undergoes drastic change in its shape, usually at the middle of the cell, pulling the two opposite membrane sides inward, closer to each other, and, finally, culminating in pinching off to separate the cell into two daughter cells. To achieve this, a cell needs to employ a protein machinery, usually termed divisome, that can coordinate all necessary intracellular processes with membrane remodelling and synthesis of other extracellular structures that decorate a cell. The focus of this dissertation is a membrane-remodelling FtsZ system that is present across all domains of life. FtsZ forms filaments that further self-organize into ring-like structures at the cell septum and together with other division proteins perform cell envelope synthesis and constriction. However, there are still knowledge gaps in our mechanistic understanding of division in both archaea and bacteria. My work presented in this dissertation centres around a simple yet not well understood question: How is the divisome positioned correctly at the mid-cell? To achieve the proper positioning, the divisome needs to (i) be recruited to the mid-cell and (ii) localized orthogonally to the long cell axis. I tackle these processes in two different systems by applying an in vitro biochemical bottom-up reconstitution approach. I use purified components of Haloferax volcanii and Escherichia coli divisome to explore how divisome is recruited to the mid-cell in archaea and how the Z-ring positions orthogonally to the long cell axis in bacteria, respectively. \r\n\r\nFirstly, I collaborate with archaeal cell and structural biologists to explore the assembly of early division proteins in two FtsZ-containing archaeon H. volcanii, a standard model system for understudied archaeal organisms. I particularly address the hierarchy of interactions that allow a tripartite complex formation (SepF-CdpB1-CdpB2) and how the hierarchy of interactions ultimately leads to the recruitment of FtsZ filaments to the septum. This part of work has been published in (Nußbaum et al., 2024). In collaboration with evolutionary biologists, I shed light on ancient features that archaeal divisome has retained to this day and also speculate on a property that it might have lost during the course of evolution. \r\n\r\nNext, I switch my attention to E. coli divisome. Particularly, I address the FtsZ’s intrinsic biophysical property that drives the Z-ring diameter, and thereby the perpendicular orientation of the Z-ring to the long cell axis based on suggested membrane curvature sensing mechanism (Vanhille-Campos et al., 2024). This property allows formation of different Z-ring diameters that match the variety of cell diameters present in prokaryotes. The results showcase that the distribution of charged amino acids in the intrinsically disordered linker at the C-terminus (CTL) of FtsZ is the major determining factor of Z-ring diameter with inter-CTL interactions as an underlying mechanism. \r\n\r\nFinally, I thoroughly explain the methodology I used to address the abovementioned projects, and I finish with a discussion on how early archaeal divisome assembly and curvature sensing mechanism in bacteria, at first sight unrelated topics, are interconnected and important groundwork for both fundamental and translational research. " acknowledged_ssus: - _id: Bio alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Marko full_name: Kojic, Marko id: 73e7ecd4-dc85-11ea-9058-88a16394b160 last_name: Kojic orcid: 0000-0001-7244-8128 citation: ama: Kojic M. Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. 2025. doi:10.15479/AT-ISTA-20741 apa: Kojic, M. (2025). Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20741 chicago: Kojic, Marko. “Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20741. ieee: M. Kojic, “Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria,” Institute of Science and Technology Austria, 2025. ista: Kojic M. 2025. Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. Institute of Science and Technology Austria. mla: Kojic, Marko. Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20741. short: M. Kojic, Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-12-09T13:08:11Z date_published: 2025-12-09T00:00:00Z date_updated: 2026-10-16T10:35:47Z day: '09' ddc: - '572' degree_awarded: PhD department: - _id: GradSch - _id: MaLo doi: 10.15479/AT-ISTA-20741 file: - access_level: closed checksum: a3643d07e93134b2490a566b02a4517d content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: mkojic date_created: 2025-12-10T13:09:58Z date_updated: 2025-12-10T13:09:58Z file_id: '20774' file_name: 2025_marko_kojic_thesis.docx file_size: 142876975 relation: source_file - access_level: open_access checksum: 0a096f0af6ccc3a8329d5bb8797ad533 content_type: application/pdf creator: mkojic date_created: 2025-12-10T13:09:38Z date_updated: 2025-12-10T13:09:38Z file_id: '20775' file_name: 2025_marko_kojic_thesis.pdf file_size: 8597045 relation: main_file success: 1 file_date_updated: 2025-12-10T13:09:58Z fulldoi: https://doi.org/10.15479/AT-ISTA-20741 has_accepted_license: '1' language: - iso: eng month: '12' oa: 1 oa_version: Published Version publication_identifier: isbn: - 978-3-99078-073-2 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '15118' relation: part_of_dissertation status: public status: public supervisor: - first_name: Martin full_name: Loose, Martin id: 462D4284-F248-11E8-B48F-1D18A9856A87 last_name: Loose orcid: 0000-0001-7309-9724 title: Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher OA_type: gold _id: '19393' abstract: - lang: eng text: "Rotations constitute one of the fundamental symmetries in physics, characterized by their intricate group structure and infinite dimensional representations. In contrast to classical rotations, quantum mechanics unveils the SO(3) symmetry group structure, manifesting in phenomena without classical counterparts, from angular momentum quantization to non-trivial addition of angular momenta.\r\nWhile most studies of topological physics have focused on two-band systems, the SO(3) symmetry group of quantum rotors offers an inherently more complex platform with unprecedented possibilities for exploring topological phenomena. Despite their ubiquity in nature– from molecules to nanorotors– their potential for hosting topological phases has remained largely unexamined.\r\nIn this thesis, we mainly focus on periodically driven linear molecules as a prototype for studying topological phenomena in quantum rotors. Recent technological advances in coherent control of molecules, particularly through precisely shaped laser pulses, have made it possible to investigate linear rotors in the context of topology. While planar rotors have received some attention in recent years, threedimensional rotors–particularly linear molecules–harbor substantially richer topological phenomena due to their non-abelian nature and their additional angular degrees of freedom. We demonstrate that these systems can host novel edge states and topological features fundamentally impossible in planar systems.\r\nWe begin by establishing a theoretical bridge between periodically kicked rotors and \"crystalline\" lattices in angular momentum space. Using non-interacting linear molecules as our primary example, we show how quantum interference and revival patterns lead to the possibility to simulate band models with arbitrary number of bands N. While our framework applies to various quantum rotors, including nanorotors and kicked Bose-Einstein condensates, linear\r\nmolecules provide an ideal experimental platform due to their abovementioned precise controllability.\r\nThe core of this work examines adiabatic dynamics of 3D quantum rotors, establishing a geometric framework based on the Euler class to characterize its non-abelian topology. The non-Hermitian nature of the system enables novel braiding behaviors and topological transitions impossible in static systems, leading to an anomalous Dirac string phase with edge states in each gap, even though the Berry phases are all zero. These features can be directly observed through\r\nmolecular alignment and rotational level populations.\r\nThese findings establish quantum rotors as an alternative platform for studying multi-band topological physics, while suggesting practical implementations for quantum computation where topological protection could offer natural resilience against decoherence. The rich structure of three-dimensional rotation groups, combined with the tunability of topological features through driving parameters, makes this platform particularly valuable for exploring fundamental\r\nphysics and developing quantum technologies." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Volker full_name: Karle, Volker id: D7C012AE-D7ED-11E9-95E8-1EC5E5697425 last_name: Karle orcid: 0000-0002-6963-0129 citation: ama: Karle V. Non-equilibrium topological phases with periodically driven molecules and quantum rotors. 2025. doi:10.15479/AT-ISTA-19393 apa: Karle, V. (2025). Non-equilibrium topological phases with periodically driven molecules and quantum rotors. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19393 chicago: Karle, Volker. “Non-Equilibrium Topological Phases with Periodically Driven Molecules and Quantum Rotors.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19393. ieee: V. Karle, “Non-equilibrium topological phases with periodically driven molecules and quantum rotors,” Institute of Science and Technology Austria, 2025. ista: Karle V. 2025. Non-equilibrium topological phases with periodically driven molecules and quantum rotors. Institute of Science and Technology Austria. mla: Karle, Volker. Non-Equilibrium Topological Phases with Periodically Driven Molecules and Quantum Rotors. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19393. short: V. Karle, Non-Equilibrium Topological Phases with Periodically Driven Molecules and Quantum Rotors, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-03-12T13:04:59Z date_published: 2025-03-13T00:00:00Z date_updated: 2026-10-16T12:16:52Z day: '13' ddc: - '530' degree_awarded: PhD department: - _id: GradSch - _id: MiLe doi: 10.15479/AT-ISTA-19393 file: - access_level: open_access checksum: d3ab25782c7ea38ce9910e57d25f6733 content_type: application/pdf creator: vkarle date_created: 2025-03-12T12:56:46Z date_updated: 2025-03-12T12:56:46Z file_id: '19394' file_name: thesis_final.pdf file_size: 10625143 relation: main_file success: 1 - access_level: closed checksum: 3ccfb0aeba4d860d71e18347913034e4 content_type: application/zip creator: vkarle date_created: 2025-03-13T13:15:10Z date_updated: 2025-03-20T08:02:35Z file_id: '19400' file_name: thesis.zip file_size: 23119202 relation: source_file file_date_updated: 2025-03-20T08:02:35Z fulldoi: https://doi.org/10.15479/AT-ISTA-19393 has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '03' oa: 1 oa_version: Published Version page: '192' publication_identifier: eissn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12788' relation: part_of_dissertation status: public - id: '9903' relation: part_of_dissertation status: public - id: '19425' relation: part_of_dissertation status: public - id: '14851' relation: part_of_dissertation status: public - id: '15004' relation: part_of_dissertation status: public status: public supervisor: - first_name: Mikhail full_name: Lemeshko, Mikhail id: 37CB05FA-F248-11E8-B48F-1D18A9856A87 last_name: Lemeshko orcid: 0000-0002-6990-7802 title: Non-equilibrium topological phases with periodically driven molecules and quantum rotors tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher OA_type: hybrid _id: '18879' abstract: - lang: eng text: 'Our brain has remarkable computational power, generating sophisticated behaviors, storing memories over an individual’s lifetime, and producing higher cognitive functions. However, little of our neuroscience knowledge covers the human brain. Is this organ truly unique, or is it a scaled version of the extensively studied rodent brain? Combining multicellular patch-clamp recording with expansion-based superresolution microscopy and full-scale modeling, we determined the cellular and microcircuit properties of the human hippocampal CA3 region, a fundamental circuit for memory storage. In contrast to neocortical networks, human hippocampal CA3 displayed sparse connectivity, providing a circuit architecture that maximizes associational power. Human synapses showed unique reliability, high precision, and long integration times, exhibiting both species- and circuit-specific properties. Together with expanded neuronal numbers, these circuit characteristics greatly enhanced the memory storage capacity of CA3. Our results reveal distinct microcircuit properties of the human hippocampus and begin to unravel the inner workings of our most complex organ. ' acknowledged_ssus: - _id: Bio - _id: PreCl - _id: LifeSc - _id: M-Shop - _id: ScienComp acknowledgement: We thank Florian Marr for excellent technical assistance, Christina Altmutter and Julia Flor for technical support, Alois Schlögl for programming, Todor Asenov for development of the transportation box for human brain tissue, Tim Vogels for guidance on simulations, Marcus Huber for mathematical advice, Walter Kaufmann for assistance with handling frozen tissue, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA, and we are grateful for assistance from Christoph Sommer and the Imaging and Optics Facility, Preclinical Facility, Lab Support Facility, Miba Machine Shop, and Scientific Computing. We are particularly grateful to the patient donors for their support of this project and also acknowledge the excellent support of the Medical University of Vienna Department of Neurosurgery staff; Romana Hoeftberger and the Division of Neuropathology and Neurochemistry; Gregor Kasprian and the Division of Neuroradiology and Musculoskeletal Radiology; and Christoph Baumgartner, Martha Feucht, and Ekaterina Pataraia for their clinical care of the patients included in this study. We thank Laura Jonkman, the NABCA biobank, and postmortem brain sample donors for their support of this research. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (advanced grant no. 692692 to P.J. and Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 to J.F.W.), the Austrian Science Fund (FWF; grant PAT 4178023 to P.J. and grant DK W1232 to M.R.T. and J.G.D.), the Austrian Academy of Sciences (DOC fellowship 26137 to M.R.T.), and a NOMIS-ISTA fellowship (to A.N.-O.). article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Jake full_name: Watson, Jake id: 63836096-4690-11EA-BD4E-32803DDC885E last_name: Watson orcid: 0000-0002-8698-3823 - first_name: Victor M full_name: Vargas Barroso, Victor M id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87 last_name: Vargas Barroso - first_name: Rebecca full_name: Morse, Rebecca id: ceb89ae7-dc8d-11ea-abe3-da3301d0eab4 last_name: Morse - first_name: Andrea C full_name: Navas Olivé, Andrea C id: 739d26c9-52e8-11ee-8d72-f14d3893b4ce last_name: Navas Olivé orcid: 0000-0002-9280-8597 - first_name: Mojtaba full_name: Tavakoli, Mojtaba id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87 last_name: Tavakoli orcid: 0000-0002-7667-6854 - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 - first_name: Matthias full_name: Tomschik, Matthias last_name: Tomschik - first_name: Karl full_name: Rössler, Karl last_name: Rössler - first_name: Peter M full_name: Jonas, Peter M id: 353C1B58-F248-11E8-B48F-1D18A9856A87 last_name: Jonas orcid: 0000-0001-5001-4804 citation: ama: Watson J, Vargas Barroso VM, Morse R, et al. Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory. Cell. 2025;188(2):501-514.e18. doi:10.1016/j.cell.2024.11.022 apa: Watson, J., Vargas Barroso, V. M., Morse, R., Navas Olivé, A. C., Tavakoli, M., Danzl, J. G., … Jonas, P. M. (2025). Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory. Cell. Cell Press. https://doi.org/10.1016/j.cell.2024.11.022 chicago: Watson, Jake, Victor M Vargas Barroso, Rebecca Morse, Andrea C Navas Olivé, Mojtaba Tavakoli, Johann G Danzl, Matthias Tomschik, Karl Rössler, and Peter M Jonas. “Human Hippocampal CA3 Uses Specific Functional Connectivity Rules for Efficient Associative Memory.” Cell. Cell Press, 2025. https://doi.org/10.1016/j.cell.2024.11.022. ieee: J. Watson et al., “Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory,” Cell, vol. 188, no. 2. Cell Press, p. 501–514.e18, 2025. ista: Watson J, Vargas Barroso VM, Morse R, Navas Olivé AC, Tavakoli M, Danzl JG, Tomschik M, Rössler K, Jonas PM. 2025. Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory. Cell. 188(2), 501–514.e18. mla: Watson, Jake, et al. “Human Hippocampal CA3 Uses Specific Functional Connectivity Rules for Efficient Associative Memory.” Cell, vol. 188, no. 2, Cell Press, 2025, p. 501–514.e18, doi:10.1016/j.cell.2024.11.022. short: J. Watson, V.M. Vargas Barroso, R. Morse, A.C. Navas Olivé, M. Tavakoli, J.G. Danzl, M. Tomschik, K. Rössler, P.M. Jonas, Cell 188 (2025) 501–514.e18. corr_author: '1' date_created: 2025-01-26T23:01:49Z date_published: 2025-01-23T00:00:00Z date_updated: 2026-10-20T08:20:04Z day: '23' ddc: - '570' department: - _id: JoDa - _id: PeJo - _id: GradSch doi: 10.1016/j.cell.2024.11.022 ec_funded: 1 external_id: isi: - '001408395600001' pmid: - '39667938' file: - access_level: open_access checksum: d5a818edc32d249cdf75e1bb5b70a4b7 content_type: application/pdf creator: dernst date_created: 2025-01-27T08:46:33Z date_updated: 2025-01-27T08:46:33Z file_id: '18884' file_name: 2025_Cell_Watson.pdf file_size: 14082343 relation: main_file success: 1 file_date_updated: 2025-01-27T08:46:33Z fulldoi: https://doi.org/10.1016/j.cell.2024.11.022 has_accepted_license: '1' intvolume: ' 188' isi: 1 issue: '2' language: - iso: eng month: '01' oa: 1 oa_version: Published Version page: 501-514.e18 pmid: 1 project: - _id: 25B7EB9E-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '692692' name: Biophysics and circuit function of a giant cortical glutamatergic synapse - _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9 call_identifier: H2020 grant_number: '101026635' name: Synaptic computations of the hippocampal CA3 circuitry - _id: 6285a163-2b32-11ec-9570-8e204ca2dba5 grant_number: '26137' name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy - _id: 2548AE96-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232 name: Molecular Drug Targets - _id: 8d9195e9-16d5-11f0-9cad-d075be887a1e grant_number: PAT 4178023 name: Synaptic networks of human brain - _id: 9B861AAC-BA93-11EA-9121-9846C619BF3A name: NOMIS Fellowship Program publication: Cell publication_identifier: eissn: - 1097-4172 issn: - 0092-8674 publication_status: published publisher: Cell Press quality_controlled: '1' related_material: record: - id: '18688' relation: earlier_version status: public scopus_import: '1' status: public title: Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 188 year: '2025' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '19795' abstract: - lang: eng text: Super-resolution microscopy often entails long acquisition times of minutes to hours. Since drifts during the acquisition adversely affect data quality, active sample stabilization is commonly used for some of these techniques to reach their full potential. Although drifts in the lateral plane can often be corrected after acquisition, this is not always possible or may come with drawbacks. Therefore, it is appealing to stabilize sample position in three dimensions (3D) during acquisition. Various schemes for active sample stabilization have been demonstrated previously, with some reaching sub-nanometer stability in 3D. Here, we present a scheme for active drift correction that delivers the nanometer-scale 3D stability demanded by state-of-the-art super-resolution techniques and is straightforward to implement compared to previous schemes capable of reaching this level of stabilization precision. Using a refined algorithm that can handle various types of reference structure, without sparse signal peaks being mandatory, we stabilized sample position to ∼1 nm in 3D using objective lenses both with high and low numerical aperture. Our implementation requires only the addition of a simple widefield imaging path and we provide an open-source control software with graphical user interface to facilitate easy adoption of the module. Finally, we demonstrate how this has the potential to enhance data collection for diffraction-limited and super-resolution imaging techniques using single-molecule localization microscopy and cryo-confocal imaging as showcases. acknowledged_ssus: - _id: M-Shop - _id: EM-Fac - _id: LifeSc acknowledgement: 'We acknowledge expert support by ISTA’s scientific service units, including the Miba Machine Shop, the Electron Microscopy Facility, and the Lab Support Facility. This work has been made possible in part by CZI grant DAF2021-234754 and grant DOI: https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (funder DOI: https://doi.org/10.13039/100014989) (F.K.M.S. and J.G.D.). We further gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant DK W1232 (M.R.T. and J.G.D.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.L.); ISTA postdoctoral fellowship IST fellow (A.W.); and Human Frontier Science Program postdoctoral fellowship LT000557/2018 (W.J.).' article_number: '100211' article_processing_charge: Yes article_type: original author: - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 - first_name: Nikolai full_name: Semenov, Nikolai id: e64d39c7-72ef-11ef-b75a-ee3046860d1b last_name: Semenov - first_name: Caroline full_name: Kreuzinger, Caroline id: 382077BA-F248-11E8-B48F-1D18A9856A87 last_name: Kreuzinger - first_name: Manjunath full_name: Javoor, Manjunath id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2 last_name: Javoor orcid: 0000-0003-2311-2112 - first_name: Bettina full_name: Zens, Bettina id: 45FD126C-F248-11E8-B48F-1D18A9856A87 last_name: Zens orcid: 0000-0002-9561-1239 - first_name: Nathalie full_name: Agudelo Duenas, Nathalie id: 40E7F008-F248-11E8-B48F-1D18A9856A87 last_name: Agudelo Duenas - first_name: Mojtaba full_name: Tavakoli, Mojtaba id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87 last_name: Tavakoli orcid: 0000-0002-7667-6854 - first_name: Marek full_name: Suplata, Marek id: EE8452B8-C26A-11E9-B157-E80CE6697425 last_name: Suplata - first_name: Wiebke full_name: Jahr, Wiebke id: 425C1CE8-F248-11E8-B48F-1D18A9856A87 last_name: Jahr orcid: 0000-0003-0201-2315 - first_name: Julia full_name: Lyudchik, Julia id: 46E28B80-F248-11E8-B48F-1D18A9856A87 last_name: Lyudchik - first_name: Andreas full_name: Wartak, Andreas id: 60aaa06c-3de5-11eb-9e53-baa88e955dcb last_name: Wartak - first_name: Florian Km full_name: Schur, Florian Km id: 48AD8942-F248-11E8-B48F-1D18A9856A87 last_name: Schur orcid: 0000-0003-4790-8078 - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 citation: ama: Vorlaufer J, Semenov N, Kreuzinger C, et al. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. Biophysical Reports. 2025;5(2). doi:10.1016/j.bpr.2025.100211 apa: Vorlaufer, J., Semenov, N., Kreuzinger, C., Javoor, M., Zens, B., Agudelo Duenas, N., … Danzl, J. G. (2025). Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. Biophysical Reports. Cell Press. https://doi.org/10.1016/j.bpr.2025.100211 chicago: Vorlaufer, Jakob, Nikolai Semenov, Caroline Kreuzinger, Manjunath Javoor, Bettina Zens, Nathalie Agudelo Duenas, Mojtaba Tavakoli, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” Biophysical Reports. Cell Press, 2025. https://doi.org/10.1016/j.bpr.2025.100211. ieee: J. Vorlaufer et al., “Image-based 3D active sample stabilization on the nanometer scale for optical microscopy,” Biophysical Reports, vol. 5, no. 2. Cell Press, 2025. ista: Vorlaufer J, Semenov N, Kreuzinger C, Javoor M, Zens B, Agudelo Duenas N, Tavakoli M, Suplata M, Jahr W, Lyudchik J, Wartak A, Schur FK, Danzl JG. 2025. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. Biophysical Reports. 5(2), 100211. mla: Vorlaufer, Jakob, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” Biophysical Reports, vol. 5, no. 2, 100211, Cell Press, 2025, doi:10.1016/j.bpr.2025.100211. short: J. Vorlaufer, N. Semenov, C. Kreuzinger, M. Javoor, B. Zens, N. Agudelo Duenas, M. Tavakoli, M. Suplata, W. Jahr, J. Lyudchik, A. Wartak, F.K. Schur, J.G. Danzl, Biophysical Reports 5 (2025). corr_author: '1' date_created: 2025-06-08T22:01:22Z date_published: 2025-06-11T00:00:00Z date_updated: 2026-10-20T08:55:34Z day: '11' ddc: - '570' department: - _id: JoDa - _id: GradSch - _id: FlSc - _id: EM-Fac doi: 10.1016/j.bpr.2025.100211 ec_funded: 1 file: - access_level: open_access checksum: 4018c833f25a3ad3b57e3577fed70334 content_type: application/pdf creator: dernst date_created: 2025-06-10T07:24:46Z date_updated: 2025-06-10T07:24:46Z file_id: '19802' file_name: 2025_BiophysicalReports_Vorlaufer.pdf file_size: 7238179 relation: main_file success: 1 file_date_updated: 2025-06-10T07:24:46Z fulldoi: https://doi.org/10.1016/j.bpr.2025.100211 has_accepted_license: '1' intvolume: ' 5' issue: '2' language: - iso: eng month: '06' oa: 1 oa_version: Published Version project: - _id: 62909c6f-2b32-11ec-9570-e1476aab5308 grant_number: CZI01 name: CryoMinflux-guided in-situ molecular census and structure determination - _id: 6285a163-2b32-11ec-9570-8e204ca2dba5 grant_number: '26137' name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program - _id: 26AA4EF2-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232-B24 name: Molecular Drug Targets - _id: 2668BFA0-B435-11E9-9278-68D0E5697425 grant_number: LT00057 name: High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration publication: Biophysical Reports publication_identifier: eissn: - 2667-0747 publication_status: published publisher: Cell Press quality_controlled: '1' related_material: record: - id: '20206' relation: dissertation_contains status: public - id: '22744' relation: dissertation_contains status: public scopus_import: '1' status: public title: Image-based 3D active sample stabilization on the nanometer scale for optical microscopy tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 5 year: '2025' ... --- OA_place: publisher _id: '20206' abstract: - lang: eng text: "The internal structure of biomolecules and their organization in higher-order arrangements are key factors governing the working principles of biological systems. Bioimaging has successfully revealed arrangements across relevant spatial scales. For example, cryo-electron tomography has become widely used for analyzing biomolecular structures in situ due to its comprehensive structural visualization of near-natively preserved samples, and its capability of sub-nm resolution via averaging. However, the identification of molecules within crowded cellular environments is often hindered by low contrast. Fluorescence microscopy, on the other hand, routinely visualizes specifically labeled targets at single-molecule contrast against essentially zero background. Moreover, it provides comparatively high throughput and is amenable to multiplexing. Due to this complementarity, combining datasets from both modalities acquired on the same region via correlative light and electron microscopy can reveal novel types of information. \r\nThe spatial scale at which information can be extracted depends on imaging resolution and correlation accuracy. Since diffraction of light limits the resolution of conventional fluorescence microscopy to few hundreds of nanometers, reaching the full potential of correlative imaging requires super-resolution approaches. Performing imaging at cryogenic temperature preserves structures in a near-native state and minimizes distortions between the fluorescence and the electron microscopy datasets. Implementations of this concept have achieved correlation on the scale of cellular organelles or bacterial domains.\r\nWe have worked towards pushing correlative imaging to the single-molecule scale by improving cryo-super-resolution microscopy, and devising a refined image correlation workflow. As part of this project, I constructed a microscopy setup and adopted it for super-resolution fluorescence microscopy at room temperature and cryogenic conditions. I explored different cryo-stages and acquisition strategies. Specifically, I developed a new scheme for correcting sample drift, thus increasing mechanical stability during microscopy acquisitions.\r\n" acknowledged_ssus: - _id: M-Shop - _id: EM-Fac - _id: Bio acknowledgement: "The project was supported by CZI grant DAF2021-234754 and grant\r\nDOI: https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an\r\nadvised fund of Silicon Valley Community Foundation (funder\r\nDOI: https://doi.org/10.13039/100014989), as well as internal grants from ISTA’s Equipment\r\nInvestment Committee and Interdisciplinary Project Committee. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 citation: ama: Vorlaufer J. Construction of a cryo-super-resolution microscope to guide in situ structure analysis. 2025. doi:10.15479/AT-ISTA-20206 apa: Vorlaufer, J. (2025). Construction of a cryo-super-resolution microscope to guide in situ structure analysis. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20206 chicago: Vorlaufer, Jakob. “Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20206. ieee: J. Vorlaufer, “Construction of a cryo-super-resolution microscope to guide in situ structure analysis,” Institute of Science and Technology Austria, 2025. ista: Vorlaufer J. 2025. Construction of a cryo-super-resolution microscope to guide in situ structure analysis. Institute of Science and Technology Austria. mla: Vorlaufer, Jakob. Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20206. short: J. Vorlaufer, Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-08-22T08:12:55Z date_published: 2025-08-25T00:00:00Z date_updated: 2026-10-20T08:55:33Z day: '25' ddc: - '621' - '535' degree_awarded: PhD department: - _id: GradSch - _id: JoDa doi: 10.15479/AT-ISTA-20206 file: - access_level: closed checksum: 191db3367c19c9b32b65f4bc3a7c19de content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: jvorlauf date_created: 2025-08-25T13:49:55Z date_updated: 2025-08-25T13:49:55Z file_id: '20228' file_name: 2025_Vorlaufer_Jakob_Thesis.docx file_size: 39735535 relation: source_file - access_level: open_access checksum: 104400e6036921569610230c1d4899dc content_type: application/pdf creator: jvorlauf date_created: 2025-08-25T13:49:56Z date_updated: 2025-08-25T13:49:56Z file_id: '20229' file_name: 2025_Vorlaufer_Jakob_Thesis.pdf file_size: 10947446 relation: main_file success: 1 file_date_updated: 2025-08-25T13:49:56Z fulldoi: https://doi.org/10.15479/AT-ISTA-20206 has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-sa/4.0/ month: '08' oa: 1 oa_version: Published Version page: '107' project: - _id: 62909c6f-2b32-11ec-9570-e1476aab5308 grant_number: CZI01 name: CryoMinflux-guided in-situ molecular census and structure determination publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19795' relation: part_of_dissertation status: public status: public supervisor: - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 title: Construction of a cryo-super-resolution microscope to guide in situ structure analysis tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '19702' abstract: - lang: eng text: Moran Birth-death process is a standard stochastic process that is used to model natural selection in spatially structured populations. A newly occurring mutation that invades a population of residents can either fixate on the whole population or it can go extinct due to random drift. The duration of the process depends not only on the total population size n, but also on the spatial structure of the population. In this work, we consider the Moran process with a single type of individuals who invade and colonize an otherwise empty environment. Mathematically, this corresponds to the setting where the residents have zero reproduction rate, thus they never reproduce. The spatial structure is represented by a graph. We present two main contributions. First, in contrast to the Moran process in which residents do reproduce, we show that the colonization time is always at most a polynomial function of the population size n. Namely, we show that colonization always takes at most 1/2n^3 - 1/2n^2 expected steps, and for each n, we identify the slowest graph where it takes exactly that many steps. Moreover, we establish a stronger bound of roughly n^2.5 steps for undirected graphs and an even stronger bound of roughly n^2 steps for so-called regular graphs. Second, we discuss various complications that one faces when attempting to measure fixation times and colonization times in spatially structured populations, and we propose to measure the real duration of the process, rather than counting the steps of the classic Moran process. article_processing_charge: Yes article_type: original arxiv: 1 author: - first_name: Lenka full_name: Kopfová, Lenka id: 17691681-50b9-11ef-ad56-edf4cacb21b0 last_name: Kopfová - first_name: Josef full_name: Tkadlec, Josef id: 3F24CCC8-F248-11E8-B48F-1D18A9856A87 last_name: Tkadlec orcid: 0000-0002-1097-9684 citation: ama: Kopfová L, Tkadlec J. Colonization times in Moran process on graphs. PLoS Computational Biology. 2025;21(5):e1012868. doi:10.1371/journal.pcbi.1012868 apa: Kopfová, L., & Tkadlec, J. (2025). Colonization times in Moran process on graphs. PLoS Computational Biology. Public Library of Science. https://doi.org/10.1371/journal.pcbi.1012868 chicago: Kopfová, Lenka, and Josef Tkadlec. “Colonization Times in Moran Process on Graphs.” PLoS Computational Biology. Public Library of Science, 2025. https://doi.org/10.1371/journal.pcbi.1012868. ieee: L. Kopfová and J. Tkadlec, “Colonization times in Moran process on graphs,” PLoS Computational Biology, vol. 21, no. 5. Public Library of Science, p. e1012868, 2025. ista: Kopfová L, Tkadlec J. 2025. Colonization times in Moran process on graphs. PLoS Computational Biology. 21(5), e1012868. mla: Kopfová, Lenka, and Josef Tkadlec. “Colonization Times in Moran Process on Graphs.” PLoS Computational Biology, vol. 21, no. 5, Public Library of Science, 2025, p. e1012868, doi:10.1371/journal.pcbi.1012868. short: L. Kopfová, J. Tkadlec, PLoS Computational Biology 21 (2025) e1012868. date_created: 2025-05-18T22:02:50Z date_published: 2025-05-01T00:00:00Z date_updated: 2026-10-20T09:45:59Z day: '01' ddc: - '000' department: - _id: GradSch doi: 10.1371/journal.pcbi.1012868 external_id: arxiv: - '2410.09476' isi: - '001481670600002' file: - access_level: open_access checksum: 73e35151eebd5064972c5a07ffdf2b69 content_type: application/pdf creator: dernst date_created: 2025-05-19T07:45:31Z date_updated: 2025-05-19T07:45:31Z file_id: '19709' file_name: 2025_PloSCompBio_Kopfova.pdf file_size: 6805943 relation: main_file success: 1 file_date_updated: 2025-05-19T07:45:31Z fulldoi: https://doi.org/10.1371/journal.pcbi.1012868 has_accepted_license: '1' intvolume: ' 21' isi: 1 issue: '5' language: - iso: eng month: '05' oa: 1 oa_version: Published Version page: e1012868 publication: PLoS Computational Biology publication_identifier: eissn: - 1553-7358 publication_status: published publisher: Public Library of Science quality_controlled: '1' scopus_import: '1' status: public title: Colonization times in Moran process on graphs tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 21 year: '2025' ... --- OA_place: publisher _id: '20203' abstract: - lang: eng text: Tribocharging, or contact electrification, is the phenomenon in which two initially neutral materials exchange electric charge through contact and subsequent separation. While it is widely observed in everyday life and crucial to numerous natural processes, even the most basic aspects of tribocharging are still a mystery—what are the charge carriers involved and what drives their exchange? This work spans three separate projects that address different aspects of tribocharging. First, we introduce a novel strategy combining Finite Element Method (FEM) simulations with Kelvin Probe Force Microscopy (KPFM) to quantitatively extract surface charge density from surface voltage maps. Second, we present a simple theoretical model that allows for the existence of triboelectric cycles, under the assumption that multiple charge carrying species are involved. Third, we present experimental evidence that identical materials can spontaneously evolve into a triboelectric series, driven by contact history. Modeling this behavior enables the replication of experimental results with simulations, and even experimentally forcing the appearance of a pre-designed series by manipulating contact history. Together, the findings from these projects challenge traditional views on tribocharging, provide new tools for probing it, and open up new avenues of research—all with the hopes of bringing us closer to understanding this puzzling phenomenon. acknowledged_ssus: - _id: M-Shop - _id: NanoFab - _id: EM-Fac - _id: LifeSc - _id: ScienComp acknowledgement: "The project in Chapter 2 has received funding from the European Research Council (ERC) under\r\nthe European Union’s Horizon 2020 research and innovation programme (Grant Agreement\r\nNo. 949120).\r\nThe project in Chapter 3 has received funding from the European Research Council (ERC) under\r\nthe European Union’s Horizon 2020 research and innovation programme (Grant Agreement\r\nNo. 949120).\r\nThe project in Chapter 4 has received financing from the European Research Council grant\r\nagreement No. 949120 under the European Union’s Horizon 2020 research and innovation\r\nprogramme. The Analytical Instrumentation Center of the TU Wien acknowledges support by\r\nthe FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state\r\nof Lower Austria and the European Regional Development Fund under grant no. WST3-F542638/004-2021.\r\n" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Juan Carlos A full_name: Sobarzo Ponce, Juan Carlos A id: 4B807D68-AE37-11E9-AC72-31CAE5697425 last_name: Sobarzo Ponce citation: ama: 'Sobarzo Ponce JCA. Tribocharging of identical insulators: Triboelectric series, triboelectric cycles and surface charges. 2025. doi:10.15479/AT-ISTA-20203' apa: 'Sobarzo Ponce, J. C. A. (2025). Tribocharging of identical insulators: Triboelectric series, triboelectric cycles and surface charges. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20203' chicago: 'Sobarzo Ponce, Juan Carlos A. “Tribocharging of Identical Insulators: Triboelectric Series, Triboelectric Cycles and Surface Charges.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20203.' ieee: 'J. C. A. Sobarzo Ponce, “Tribocharging of identical insulators: Triboelectric series, triboelectric cycles and surface charges,” Institute of Science and Technology Austria, 2025.' ista: 'Sobarzo Ponce JCA. 2025. Tribocharging of identical insulators: Triboelectric series, triboelectric cycles and surface charges. Institute of Science and Technology Austria.' mla: 'Sobarzo Ponce, Juan Carlos A. Tribocharging of Identical Insulators: Triboelectric Series, Triboelectric Cycles and Surface Charges. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20203.' short: 'J.C.A. Sobarzo Ponce, Tribocharging of Identical Insulators: Triboelectric Series, Triboelectric Cycles and Surface Charges, Institute of Science and Technology Austria, 2025.' corr_author: '1' date_created: 2025-08-21T11:42:59Z date_published: 2025-08-27T00:00:00Z date_updated: 2026-10-21T08:38:30Z day: '27' ddc: - '530' degree_awarded: PhD department: - _id: GradSch - _id: ScWa doi: 10.15479/AT-ISTA-20203 doi_confirm: '1' ec_funded: 1 file: - access_level: open_access checksum: 661b9d3786cfc985be811befc3262bf5 content_type: application/pdf creator: jsobarzo date_created: 2025-08-27T14:50:32Z date_updated: 2025-08-27T14:50:32Z file_id: '20237' file_name: 2025_Sobarzo_JuanCarlos_Thesis.pdf file_size: 12667200 relation: main_file success: 1 - access_level: closed checksum: ca2f24e6c3b55912982521707552a0f5 content_type: application/x-zip-compressed creator: jsobarzo date_created: 2025-08-27T14:50:32Z date_updated: 2025-08-28T08:19:07Z file_id: '20238' file_name: 2025_Sobarzo_JuanCarlos_Thesis.zip file_size: 18940521 relation: source_file file_date_updated: 2025-08-28T08:19:07Z fulldoi: https://doi.org/10.15479/AT-ISTA-20203 has_accepted_license: '1' language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: '96' project: - _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa call_identifier: H2020 grant_number: '949120' name: 'Tribocharge: a multi-scale approach to an enduring problem in physics' publication_identifier: isbn: - 978-3-99078-062-6 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12109' relation: part_of_dissertation status: public - id: '19278' relation: part_of_dissertation status: public - id: '15322' relation: part_of_dissertation status: public status: public supervisor: - first_name: Scott R full_name: Waitukaitis, Scott R id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87 last_name: Waitukaitis orcid: 0000-0002-2299-3176 title: 'Tribocharging of identical insulators: Triboelectric series, triboelectric cycles and surface charges' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher _id: '20607' acknowledged_ssus: - _id: Bio - _id: SSU - _id: LifeSc - _id: M-Shop acknowledgement: "I gratefully acknowledge the support of the ISTA Graduate School and the Scientific Service Units of\r\nISTA, whose resources made this work possible—especially the Imaging & Optics Facility, the Lab\r\nSupport Facility, and the Miba Machine Shop. I would like to thank two staff scientists in particular:\r\nRobert Hauschild (Imaging & Optics Facility) and Daniel Balazs (Lab Support Facility), for their\r\nassistance and advice. My PhD was partially funded by the Austrian Science Fund (FWF)\r\n(10.55776/P37169 and 10.55776/COE5)." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Soumyadip full_name: Mondal, Soumyadip id: d25d21ef-dc8d-11ea-abe3-ec4576307f48 last_name: Mondal citation: ama: 'Mondal S. Oxygen and sulfur redox: Conversion kinetics and phase equilibria. 2025. doi:10.15479/AT-ISTA-20607' apa: 'Mondal, S. (2025). Oxygen and sulfur redox: Conversion kinetics and phase equilibria. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20607' chicago: 'Mondal, Soumyadip. “Oxygen and Sulfur Redox: Conversion Kinetics and Phase Equilibria.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20607.' ieee: 'S. Mondal, “Oxygen and sulfur redox: Conversion kinetics and phase equilibria,” Institute of Science and Technology Austria, 2025.' ista: 'Mondal S. 2025. Oxygen and sulfur redox: Conversion kinetics and phase equilibria. Institute of Science and Technology Austria.' mla: 'Mondal, Soumyadip. Oxygen and Sulfur Redox: Conversion Kinetics and Phase Equilibria. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20607.' short: 'S. Mondal, Oxygen and Sulfur Redox: Conversion Kinetics and Phase Equilibria, Institute of Science and Technology Austria, 2025.' corr_author: '1' das_tickbox: '1' date_created: 2025-11-07T12:40:54Z date_published: 2025-09-19T00:00:00Z date_updated: 2026-10-21T10:25:04Z day: '19' ddc: - '541' - '543' - '542' degree_awarded: PhD department: - _id: GradSch - _id: StFr doi: 10.15479/AT-ISTA-20607 doi_confirm: '1' file: - access_level: closed checksum: b5eed6a3dccb83cd2a8a22e11fd7d867 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: smondal date_created: 2025-11-13T16:47:47Z date_updated: 2025-11-13T16:47:47Z file_id: '20644' file_name: 2025_Mondal_Soumyadip_Thesis.docx file_size: 32589295 relation: source_file - access_level: closed checksum: 89b1529e0a7b524f46624d73ecadf8cb content_type: application/pdf creator: smondal date_created: 2025-11-13T16:47:46Z date_updated: 2025-11-13T16:47:46Z embargo: 2026-11-13 embargo_to: open_access file_id: '20645' file_name: 2025_Mondal_Soumyadip_Thesis.pdf file_size: 5007370 relation: main_file file_date_updated: 2025-11-13T16:47:47Z fulldoi: https://doi.org/10.15479/AT-ISTA-20607 has_accepted_license: '1' language: - iso: eng month: '09' oa_version: Published Version page: '71' project: - _id: 8df062be-16d5-11f0-9cad-f559b6612c7e grant_number: P37169 name: Singlet oxygen in non-aqueous oxygen redox chemistry - _id: 5eaf4378-b033-11f1-b276-f928018a46c1 grant_number: COE05 name: Materials for Energy Conversion and Storage (Freunberger) publication_identifier: isbn: - 978-3-99078-071-8 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12065' relation: part_of_dissertation status: public - id: '20437' relation: part_of_dissertation status: deleted - id: '13044' relation: part_of_dissertation status: public - id: '14687' relation: part_of_dissertation status: public status: public supervisor: - first_name: Stefan Alexander full_name: Freunberger, Stefan Alexander id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425 last_name: Freunberger orcid: 0000-0003-2902-5319 title: 'Oxygen and sulfur redox: Conversion kinetics and phase equilibria' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- OA_place: publisher _id: '20339' abstract: - lang: eng text: "This thesis investigates the interplay between algebraic and topological methods and combinatorial problems, focusing on approximate graph colourings and mass partitioning. The unifying theme throughout the dissertation is the use of continuous maps and symmetry constraints to extract combinatorial insights.\r\n\r\nWe first explore approximate graph colouring problems and more generally promise constraint satisfaction problems. Using tools from equivariant topology in combination with the general theory of polymorphism of a promise constraint satisfaction problem, we establish hardness for specific types of approximations.\r\n\r\nIn the second part, we address mass partitioning problems, where one seeks to divide geometric objects or measures in Euclidean space into parts of equal size using hyperplanes. Employing techniques from topological combinatorics (configuration space/test map setup and Borsuk–Ulam type theorems), we both obtain a new equipartitioning result in the and provide a fast algorithm for computing equipartitioning of point sets in 3D.\r\n" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Gianluca full_name: Tasinato, Gianluca id: 0433290C-AF8F-11E9-A4C7-F729E6697425 last_name: Tasinato citation: ama: 'Tasinato G. Topological methods in discrete geometry and theoretical computer science: Measure partitioning and constraint satisfaction problems. 2025. doi:10.15479/AT-ISTA-20339' apa: 'Tasinato, G. (2025). Topological methods in discrete geometry and theoretical computer science: Measure partitioning and constraint satisfaction problems. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20339' chicago: 'Tasinato, Gianluca. “Topological Methods in Discrete Geometry and Theoretical Computer Science: Measure Partitioning and Constraint Satisfaction Problems.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20339.' ieee: 'G. Tasinato, “Topological methods in discrete geometry and theoretical computer science: Measure partitioning and constraint satisfaction problems,” Institute of Science and Technology Austria, 2025.' ista: 'Tasinato G. 2025. Topological methods in discrete geometry and theoretical computer science: Measure partitioning and constraint satisfaction problems. Institute of Science and Technology Austria.' mla: 'Tasinato, Gianluca. Topological Methods in Discrete Geometry and Theoretical Computer Science: Measure Partitioning and Constraint Satisfaction Problems. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20339.' short: 'G. Tasinato, Topological Methods in Discrete Geometry and Theoretical Computer Science: Measure Partitioning and Constraint Satisfaction Problems, Institute of Science and Technology Austria, 2025.' corr_author: '1' date_created: 2025-09-10T12:17:55Z date_published: 2025-09-10T00:00:00Z date_updated: 2026-10-21T13:23:55Z day: '10' ddc: - '516' degree_awarded: PhD department: - _id: GradSch - _id: UlWa doi: 10.15479/AT-ISTA-20339 doi_confirm: '1' file: - access_level: closed checksum: ae097a515b9bb4d4b025ca854ae2ed76 content_type: application/x-zip-compressed creator: gtasinat date_created: 2025-09-11T12:24:12Z date_updated: 2025-09-11T12:24:12Z file_id: '20344' file_name: thesis-source.zip file_size: 2218562 relation: source_file - access_level: open_access checksum: 04b2e016409e52167ce42b0eef839fbf content_type: application/pdf creator: gtasinat date_created: 2025-09-11T12:26:14Z date_updated: 2025-09-11T12:26:14Z file_id: '20345' file_name: 2025_Tasinato_Gianluca_Thesis.pdf file_size: 10071982 relation: main_file success: 1 file_date_updated: 2025-09-11T12:26:14Z fulldoi: https://doi.org/10.15479/AT-ISTA-20339 has_accepted_license: '1' language: - iso: eng month: '09' oa: 1 oa_version: Published Version page: '106' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20008' relation: part_of_dissertation status: public - id: '19860' relation: part_of_dissertation status: public - id: '15168' relation: part_of_dissertation status: public status: public supervisor: - first_name: Uli full_name: Wagner, Uli id: 36690CA2-F248-11E8-B48F-1D18A9856A87 last_name: Wagner orcid: 0000-0002-1494-0568 title: 'Topological methods in discrete geometry and theoretical computer science: Measure partitioning and constraint satisfaction problems' tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher _id: '20485' acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: "I would also like to acknowledge the funding that I received from the European Union’s\r\nHorizon 2020 research and Innovation programme under the Marie Sklodowska-Curie\r\nGrant Agreement No. 665385. This work would not have been possible without the contribution and support of people\r\nbehind the scientific service units at ISTA: the Life Science Facility (LSF), Imaging and\r\nOptics Facility (IOF), the Bioinformatics Unit, Protein Services Unit and\r\nElectrophysiology Unit. I would also like to recognize the work of people at the Vienna\r\nBiocenter (VBC) Mass Spectrometry Facility, particularly Markus Hartl and WeiQiang\r\nChen. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Michaela full_name: Misova, Michaela id: 495A3C32-F248-11E8-B48F-1D18A9856A87 last_name: Misova orcid: 0000-0003-2427-6856 citation: ama: Misova M. Dissecting gap junction biology using the C. elegans nervous system. 2025. doi:10.15479/AT-ISTA-20485 apa: Misova, M. (2025). Dissecting gap junction biology using the C. elegans nervous system. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20485 chicago: Misova, Michaela. “Dissecting Gap Junction Biology Using the C. Elegans Nervous System.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20485. ieee: M. Misova, “Dissecting gap junction biology using the C. elegans nervous system,” Institute of Science and Technology Austria, 2025. ista: Misova M. 2025. Dissecting gap junction biology using the C. elegans nervous system. Institute of Science and Technology Austria. mla: Misova, Michaela. Dissecting Gap Junction Biology Using the C. Elegans Nervous System. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20485. short: M. Misova, Dissecting Gap Junction Biology Using the C. Elegans Nervous System, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-17T16:15:09Z date_published: 2025-10-23T00:00:00Z date_updated: 2026-10-23T22:30:03Z day: '23' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: MaDe doi: 10.15479/AT-ISTA-20485 ec_funded: 1 file: - access_level: closed checksum: e042ea314e7e13fce76c6c95e126779a content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: mmisova date_created: 2025-10-23T08:22:35Z date_updated: 2026-10-23T22:30:02Z embargo_to: open_access file_id: '20518' file_name: 2025-Misova-Michaela-Thesis.docx file_size: 75070995 relation: source_file - access_level: open_access checksum: fcd8973d6a025256eb0eb1a82c02172c content_type: application/pdf creator: mmisova date_created: 2025-10-23T08:21:21Z date_updated: 2026-10-23T22:30:02Z embargo: 2026-10-23 file_id: '20519' file_name: 2025-Misova-Michaela-Thesis.pdf file_size: 10974630 relation: main_file file_date_updated: 2026-10-23T22:30:02Z fulldoi: https://doi.org/10.15479/AT-ISTA-20485 has_accepted_license: '1' language: - iso: eng month: '10' oa: 1 oa_version: Published Version page: '155' project: - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program publication_identifier: isbn: - 978-3-99078-068-8 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Mario full_name: de Bono, Mario id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87 last_name: de Bono orcid: 0000-0001-8347-0443 title: Dissecting gap junction biology using the C. elegans nervous system tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19557' acknowledged_ssus: - _id: Bio - _id: PreCl - _id: ScienComp acknowledgement: "The work presented in this doctoral thesis was performed at the Institute of Science\r\nand Technology (ISTA) and financially supported by a European Research Council\r\n(ERC) Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by the\r\nAustrian Science Fund (FWF) to Gaia Novarino (PE1028W1232). I am very thankful\r\nto the Doctoral Program “Molecular Drug Targets” (MolTag) for offering me financial\r\nsupport to perform essential experiments during my PhD studies and to participate in\r\ninternational conferences and courses." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Lena A full_name: Schwarz, Lena A id: 29A8453C-F248-11E8-B48F-1D18A9856A87 last_name: Schwarz citation: ama: Schwarz LA. Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. 2025. doi:10.15479/AT-ISTA-19557 apa: Schwarz, L. A. (2025). Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19557 chicago: Schwarz, Lena A. “Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19557. ieee: L. A. Schwarz, “Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution,” Institute of Science and Technology Austria, 2025. ista: Schwarz LA. 2025. Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. Institute of Science and Technology Austria. mla: Schwarz, Lena A. Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19557. short: L.A. Schwarz, Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-04-14T06:59:06Z date_published: 2025-04-14T00:00:00Z date_updated: 2026-10-15T22:30:03Z day: '14' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: GaNo doi: 10.15479/AT-ISTA-19557 file: - access_level: closed checksum: 50290a8604edb0a720387f01e9d59fe4 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: lschwarz date_created: 2025-04-15T08:43:36Z date_updated: 2026-10-15T22:30:02Z embargo_to: open_access file_id: '19561' file_name: Schwarz_Thesis_2025_FINAL.docx file_size: 21783427 relation: source_file - access_level: open_access checksum: ed028488180ac4901e018ef1c330cf01 content_type: application/pdf creator: lschwarz date_created: 2025-04-15T08:43:42Z date_updated: 2026-10-15T22:30:02Z embargo: 2026-10-15 file_id: '19562' file_name: Schwarz_Thesis_2025_FINALpdfa.pdf file_size: 11432175 relation: main_file file_date_updated: 2026-10-15T22:30:02Z fulldoi: https://doi.org/10.15479/AT-ISTA-19557 has_accepted_license: '1' language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: '124' project: - _id: 9B91375C-BA93-11EA-9121-9846C619BF3A grant_number: '707964' name: Critical windows and reversibility of ASD associated with mutations in chromatin remodelers - _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6 grant_number: '101044865' name: Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders - _id: 2548AE96-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232 name: Molecular Drug Targets publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12802' relation: part_of_dissertation status: public - id: '9429' relation: part_of_dissertation status: public status: public supervisor: - first_name: Gaia full_name: Novarino, Gaia id: 3E57A680-F248-11E8-B48F-1D18A9856A87 last_name: Novarino orcid: 0000-0002-7673-7178 title: Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_embargo: '6' OA_place: publisher _id: '20798' abstract: - lang: eng text: "Atom interferometers measure the relative phase shifts between coherent matter-wave paths\r\nthat arise from interactions with external fields or inertial forces. Due to their exceptional\r\nphase sensitivity, atom interferometers became an essential tool for precision measurements\r\nand fundamental physics experiments, finding applications in geodesy, gravimetry, and inertial\r\nnavigation. However, their measurement precision is limited by quantum projection noise,\r\nwhich arises from the Heisenberg uncertainty principle, preventing the measurement of atomic\r\nstates with absolute precision. The generation of entanglement between the atoms offers a\r\npath to surpass this so-called standard quantum limit, thereby enhancing the interferometer’s\r\nphase sensitivity beyond classical measurement bounds.\r\nThis thesis reports on the development of an atom interferometer experiment designed to\r\nrealize cavity-mediated, squeezed Mach-Zehnder-type interferometry with ultra-cold 87Rb atoms.\r\nThe experiment combines cavity-aided spin-squeezing with cavity-mediated Mach-Zehnder\r\ninterferometry to demonstrate entanglement-enhanced phase sensitivity. The experiment is\r\ncentered on a triangular optical cavity that mediates all relevant atom-light interactions. The\r\ncavity provides optical trapping, spin-squeezing, and Raman beam-splitter operations, enabling\r\nto perform interferometry on a continuously trapped atomic ensemble.\r\nThe thesis elaborates on the fundamental theoretical framework, the cavity design, and the full\r\noptical setup, including the detailed configuration of the developed laser stabilization methods.\r\nExperimentally, continuous loading methods were explored, resulting in an accumulation of\r\nup to 4 × 106\r\natoms in the dipole trap within a cycle time of 500 ms. The AC Stark shift\r\ncompensation method developed for continuous loading was further applied for in-trap cooling\r\nto 10 µK, and optical pumping for efficient atomic state preparation. Coherent state control\r\nwas verified via observation of microwave-driven Rabi oscillations, and used to characterize\r\natom-cavity coupling.\r\nThese presented results establish the experimental groundwork for the future development of\r\ncavity-mediated, entanglement-enhanced Mach-Zehnder-type atom interferometry." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Sebastian full_name: Wald, Sebastian id: 133F200A-B015-11E9-AD41-0EDAE5697425 last_name: Wald orcid: 0000-0002-5869-1604 citation: ama: Wald S. Atoms in a propagating-wave cavity for squeezed Mach-Zehnder atom interferometry. 2025. doi:10.15479/AT-ISTA-20798 apa: Wald, S. (2025). Atoms in a propagating-wave cavity for squeezed Mach-Zehnder atom interferometry. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20798 chicago: Wald, Sebastian. “Atoms in a Propagating-Wave Cavity for Squeezed Mach-Zehnder Atom Interferometry.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20798. ieee: S. Wald, “Atoms in a propagating-wave cavity for squeezed Mach-Zehnder atom interferometry,” Institute of Science and Technology Austria, 2025. ista: Wald S. 2025. Atoms in a propagating-wave cavity for squeezed Mach-Zehnder atom interferometry. Institute of Science and Technology Austria. mla: Wald, Sebastian. Atoms in a Propagating-Wave Cavity for Squeezed Mach-Zehnder Atom Interferometry. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20798. short: S. Wald, Atoms in a Propagating-Wave Cavity for Squeezed Mach-Zehnder Atom Interferometry, Institute of Science and Technology Austria, 2025. corr_author: '1' das_tickbox: '1' date_created: 2025-12-11T11:48:11Z date_published: 2025-12-11T00:00:00Z date_updated: 2026-07-24T08:07:28Z day: '11' ddc: - '530' degree_awarded: PhD department: - _id: GradSch - _id: OnHo doi: 10.15479/AT-ISTA-20798 doi_confirm: '1' file: - access_level: open_access checksum: 1be72faf529a5e8a2d03cb3d5f808b77 content_type: application/pdf creator: swald date_created: 2025-12-12T11:53:42Z date_updated: 2026-06-15T22:30:03Z embargo: 2026-06-15 file_id: '20809' file_name: 2025_Wald_Sebastian_Thesis.pdf file_size: 47536855 relation: main_file - access_level: closed checksum: 8c3a1904dceb4bcd04bc9f14b2594bab content_type: application/x-zip-compressed creator: swald date_created: 2025-12-12T11:54:55Z date_updated: 2026-06-15T22:30:03Z embargo_to: open_access file_id: '20810' file_name: 2025_Wald_Sebastian_Thesis.zip file_size: 40127601 relation: source_file file_date_updated: 2026-06-15T22:30:03Z fulldoi: https://doi.org/10.15479/AT-ISTA-20798 has_accepted_license: '1' keyword: - entanglement-enhanced atom interferometry - cavity QED - spin-squeezing - dipole trap - quantum optics language: - iso: eng license: https://creativecommons.org/licenses/by-nc/4.0/ month: '12' oa: 1 oa_version: Published Version page: '152' publication_identifier: isbn: - 978-3-99078-075-6 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '14759' relation: part_of_dissertation status: public status: public supervisor: - first_name: Onur full_name: Hosten, Onur id: 4C02D85E-F248-11E8-B48F-1D18A9856A87 last_name: Hosten orcid: 0000-0002-2031-204X title: Atoms in a propagating-wave cavity for squeezed Mach-Zehnder atom interferometry tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher _id: '18871' abstract: - lang: eng text: "\"Can we do this with a new type of computer - a quantum computer?\". This famous\r\nquotation of the brilliant Richard Feynman within a conference talk on \"Simulating physics\r\nwith computers.” is often reverently praised as the origin of the field of quantum computing.\r\nThe idea was to use quantum mechanical systems itself to simulate \"Nature\", which is\r\ninherently quantum mechanical. Now, 43 years later, the theoretical framework of how such\r\na computer can operate has been developed. Two main important concepts for a potential\r\nquantum supremacy, superposition and entanglement, have been exploited to design quantum\r\nalgorithms to significantly speed up certain tasks. Yet, the specific hardware implementation\r\nis still far from being certain, in fact the race between the most promising platforms such as\r\nsuperconducting qubits, bosonic codes, cold atoms, trapped ions, optical computing as well\r\nas spin qubits has recently intensified. If one also includes the most mature applications of\r\nquantum communication technologies, secure quantum key distribution and quantum random\r\nnumber generators, as part of a quantum information technology ecosystem, we are confronted\r\nwith a plethora of different materials, concepts, and also operation frequencies. While\r\nsuperconducting qubits, bosonic codes and spin qubits work in the regime of approximately 5\r\nGHz and are controlled by electrical fields, trapped ions, cold atoms, and optical quantum\r\ncomputing operate with light in the infrared or visible range.\r\nConsequently, a quantum frequency converter or microwave-optic transducer is required\r\nto interface the different frequency domains or establish a long-range network connection\r\nwith suitable telecom fibers. In fact, the combination of different frequency regimes is also\r\nan essential part in our classical modern communication network, where computations are\r\nperformed in electrical circuits and the information exchange over longer distances happens\r\nvia optical fibers. However, the specific challenges specific to building a quantum computer,\r\nalso apply to the development of such a quantum frequency transducer: 1) As we deal with\r\nsingle excitations as the carrier of information, i.e. the smallest possible quantity, the signal\r\ncan easily be corrupted by other noise sources which needs to be avoided by all means. This\r\nis also the reason why microwave quantum computers operate at temperature environments\r\nclose to zero temperature (< 0.1 Kelvin) to avoid corruption by thermal noise. 2) The\r\nfrequency interface generally needs to preserve the phase of the signal as an essential part\r\nof the quantum state. And 3) Quantum signals cannot be copied which would be a typical\r\nstrategy to account for errors in classical computers. And finally, there is a challenge specific to\r\nmicrowave-optic transducers: While quantum computers are operating in one specific frequency\r\ndomain, microwave-optic transducers combine microwave and optical fields in one device.\r\nThis results in the particular challenge that high-energy optical radiation, which is usually\r\nwell-shielded from superconducting microwave quantum processors, are now an essential part\r\nof the device. The concomitant optical radiation in the operating transducer will inevitably\r\nhave a detrimental effect on the superconducting microwave components. Together with the\r\nrequirement of minimal background noise for quantum-limited operation as described above,\r\nv\r\nheating from the absorption of optical photons within the same device where single microwave\r\nexcitations are processed forms a formidable challenge.\r\nThis thesis aims to address this challenge by developing microwave-optic transducers where\r\nthe impact of optical absorption on superconducting circuits in general and superconducting\r\nqubits specifically can be mitigated. In our first approach, we developed a compact device\r\nwith optimized interaction strengths between the different frequency domains. This minimizes\r\nthe optical powers used for transducer operation and thus the optical absorption heating. This\r\nwork was - to the best of our knowledge - the first comprehensive noise study, in an integrated\r\nmicrowave-optic transducer. Unfortunately, we saw that the optical absorption heating added\r\nnoise way above a single excitation. Consequently, a potential quantum signal would have\r\nbeen buried in the noise, added by the transduction.\r\nBuilding on this insight, we utilized a three-dimensional microwave-optic transducer instead\r\nof an integrated device. The larger heat capacity of the macroscopic device with a size\r\nof a few millimeters can absorb a larger fraction of the optical heating before it increases\r\nthe temperature of the device. This allowed us to interface the transducer directly with a\r\nsuperconducting qubit to readout the qubit state in a novel all-optical manner. We showed\r\nthat the microwave-optic transducer can be operated in a regime in which optical fields don’t\r\nharm the sensitive qubit. This is an important prerequisite for the operation of microwave-optic\r\ntransducers in conjunction with microwave quantum processors and brings the integration and\r\nseamless orchestration of different frequency components in a quantum network a step closer.\r\n" acknowledged_ssus: - _id: SSU - _id: M-Shop - _id: NanoFab acknowledgement: "This work was supported by the European Research Council under grant agreement no. 758053\r\n(ERC StG QUNNECT) and the European Union’s Horizon 2020 research, innovation program\r\nunder grant agreement no. 899354 (FETopen SuperQuLAN) and the Austrian Science Fund\r\n(FWF) through BeyondC (F7105). I want to acknowledge generous support from the Austrian\r\nAcademy of Sciences from a DOC [Doctoral program of the Austrian Academy of Sciences]\r\nfellowship (no. 25129).\r\n" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Georg M full_name: Arnold, Georg M id: 3770C838-F248-11E8-B48F-1D18A9856A87 last_name: Arnold orcid: 0000-0003-1397-7876 citation: ama: Arnold GM. Microwave-optic interconnects for superconducting circuits. 2025. doi:10.15479/at:ista:18871 apa: Arnold, G. M. (2025). Microwave-optic interconnects for superconducting circuits. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:18871 chicago: Arnold, Georg M. “Microwave-Optic Interconnects for Superconducting Circuits.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/at:ista:18871. ieee: G. M. Arnold, “Microwave-optic interconnects for superconducting circuits,” Institute of Science and Technology Austria, 2025. ista: Arnold GM. 2025. Microwave-optic interconnects for superconducting circuits. Institute of Science and Technology Austria. mla: Arnold, Georg M. Microwave-Optic Interconnects for Superconducting Circuits. Institute of Science and Technology Austria, 2025, doi:10.15479/at:ista:18871. short: G.M. Arnold, Microwave-Optic Interconnects for Superconducting Circuits, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-01-24T10:28:39Z date_published: 2025-01-24T00:00:00Z date_updated: 2026-04-16T12:20:43Z day: '24' ddc: - '530' degree_awarded: PhD department: - _id: JoFi - _id: GradSch doi: 10.15479/at:ista:18871 ec_funded: 1 file: - access_level: closed checksum: 71872702e8f46c275eaea44efc4d304f content_type: application/x-zip-compressed creator: cchlebak date_created: 2025-01-29T08:38:08Z date_updated: 2026-01-29T23:30:03Z embargo_to: open_access file_id: '18946' file_name: tex for upload.zip file_size: 18856130 relation: source_file - access_level: open_access checksum: dfaa06591970f4bff163705802fad56d content_type: application/pdf creator: cchlebak date_created: 2025-01-29T08:38:34Z date_updated: 2026-01-29T23:30:03Z embargo: 2026-01-29 file_id: '18947' file_name: ISTThesisGA2022_final.pdf file_size: 17344760 relation: main_file file_date_updated: 2026-01-29T23:30:03Z fulldoi: https://doi.org/10.15479/at:ista:18871 has_accepted_license: '1' language: - iso: eng month: '01' oa: 1 oa_version: Published Version page: '135' project: - _id: 26336814-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '758053' name: A Fiber Optic Transceiver for Superconducting Qubits - _id: 9B868D20-BA93-11EA-9121-9846C619BF3A call_identifier: H2020 grant_number: '899354' name: Quantum Local Area Networks with Superconducting Qubits - _id: 2671EB66-B435-11E9-9278-68D0E5697425 name: Coherent on-chip conversion of superconducting qubit signals from microwaves to optical frequencies - _id: bdb108fd-d553-11ed-ba76-83dc74a9864f grant_number: F07105 name: QUANTUM INFORMATION SYSTEMS BEYOND CLASSICAL CAPABILITIES / P5- Integration of Superconducting Quantum Circuits publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '6609' relation: part_of_dissertation status: public - id: '8529' relation: part_of_dissertation status: public - id: '18953' relation: part_of_dissertation status: public - id: '10924' relation: part_of_dissertation status: public - id: '9114' relation: part_of_dissertation status: public - id: '13200' relation: part_of_dissertation status: public status: public supervisor: - first_name: Johannes M full_name: Fink, Johannes M id: 4B591CBA-F248-11E8-B48F-1D18A9856A87 last_name: Fink orcid: 0000-0001-8112-028X title: Microwave-optic interconnects for superconducting circuits tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- OA_embargo: '6' OA_place: publisher _id: '19745' abstract: - lang: eng text: "Cell migration is a crucial process in animal development and maintenance. It is incredibly\r\nheterogeneous, with different cell types utilizing fundamentally distinct migration strategies.\r\nThe strategies also depend on the cellular microenvironment, where cells can switch between\r\nmigration modes as they encounter new environmental cues. In this thesis, we investigated\r\nhow dendritic cells adapt their migration strategy when encountering geometrically,\r\nmechanically and chemically distinct environments.\r\nWhen dendritic cells are embedded in a homogeneous fibrous network, they migrate in a fast\r\nand directional amoeboid manner. In this migration strategy, extracellular proteolysis and\r\nintegrin-mediated adhesions are dispensable. Instead, the cells use topography of the\r\nenvironment to propel their cell body forward. To migrate efficiently in the maze of different\r\npore sizes, they position the nucleus ahead of the microtubule organizing center (MTOC) and\r\nuse it to gauge the pores to identify the path of least resistance. Our aim was to identify\r\nwhether dendritic cells adapt their migration strategy when encountering asymmetrical\r\ntransitions into much denser environments with limited choice of large pores. In such invasive\r\ntransitions it is unclear if the cells can cross tight pores without the use of adhesions and\r\nextracellular proteolysis and whether they maintain the nucleus in the cell front.\r\nUsing various cell migration assays such as fibrous 3D collagen gels, geometrically defined\r\nmicrochannels with constrictions and simplistic under agarose migration assay, we provide\r\na comprehensive characterization of invasive migration of dendritic cells. We show that\r\nduring invasion the cells stall and stretch, reflecting the difficulty to translocate the bulky cell\r\nbody into the dense environment. In collagen gels, we show that dendritic cells can invade\r\nwithout proteolysis and adhesions. Instead, they utilize contractility, which can lead to largescale collagen compressions. During invasion, the nucleus stalls at tight constrictions, leading\r\nto a transient organelle reorientation. To resolve the stalling, upregulated rear contractility is\r\nrequired. This contractile force is simultaneously necessary for reverting the nucleus back to\r\nthe cell front after invasion and maintaining this positioning during permissive migration.\r\nA functional role of the reorientation was uncovered in the first collaboration project.\r\nA prominent central actin pool was identified around the MTOC, especially pronounced in\r\ndense and compressive environments. The actin pool was shown to generate pushing forces\r\nto dilate the space for cell translocation. These forces are only necessary in non-permissive\r\nenvironments, where the nucleus reorients to the cell rear, allowing the actin pool to\r\ngenerate space. In permissive environments where space generation is dispensable, the\r\nMTOC is located behind the nucleus and the actin cloud has reduced intensity, allowing more\r\nactin to be incorporated into the lamellipodium, speeding up migration.\r\nIn the second collaboration project, we investigated the effects of distinct chemical\r\nenvironments on dendritic cell migration. The strikingly persistent migration of these cells\r\nwas explained by their ability to modulate and even self-generate chemokine gradients. This\r\nallows the cells to migrate faster and more persistent in uniform chemokine fields compared\r\nto imposed chemokine gradients. The chemokine receptor CCR7 was identified as a crucial\r\nplayer in this process, both sensing the signal and internalizing the chemokine to create a sink." acknowledgement: "This project has received funding from the Austrian Science Fund (FWF) via the doctorate\r\ncollege DK NanoCell and from the European Union’s Horizon 2020 research and innovation\r\nprogramme under the Marie Skłodowska-Curie Grant Agreement No. 665385.\r\n" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Nikola full_name: Canigova, Nikola id: 3795523E-F248-11E8-B48F-1D18A9856A87 last_name: Canigova orcid: 0000-0002-8518-5926 citation: ama: Canigova N. Adaptive strategies of dendritic cell migration in response to environmental cues. 2025. doi:10.15479/AT-ISTA-19745 apa: Canigova, N. (2025). Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19745 chicago: Canigova, Nikola. “Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19745. ieee: N. Canigova, “Adaptive strategies of dendritic cell migration in response to environmental cues,” Institute of Science and Technology Austria, 2025. ista: Canigova N. 2025. Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria. mla: Canigova, Nikola. Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19745. short: N. Canigova, Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-05-26T08:49:00Z date_published: 2025-05-27T00:00:00Z date_updated: 2026-06-18T17:34:48Z day: '27' ddc: - '570' degree_awarded: PhD department: - _id: MiSi - _id: GradSch doi: 10.15479/AT-ISTA-19745 ec_funded: 1 file: - access_level: closed checksum: 1a2d1525d19347fbb879ef57c02951bf content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: cchlebak date_created: 2025-05-28T07:38:17Z date_updated: 2025-11-27T23:30:02Z embargo_to: open_access file_id: '19748' file_name: NikolaCanigova_Thesis_final.docx file_size: 103879193 relation: source_file - access_level: open_access checksum: c1d8f9a40a8e19fcf895373f4b773a46 content_type: application/pdf creator: cchlebak date_created: 2025-05-28T07:39:53Z date_updated: 2025-11-27T23:30:02Z embargo: 2025-11-27 file_id: '19749' file_name: NikolaCanigova_Thesis_final_PDFA2a_fixed.pdf file_size: 194530600 relation: main_file file_date_updated: 2025-11-27T23:30:02Z fulldoi: https://doi.org/10.15479/AT-ISTA-19745 has_accepted_license: '1' language: - iso: eng month: '05' oa: 1 oa_version: Published Version page: '133' project: - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program - _id: 265E2996-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W01250-B20 name: Nano-Analytics of Cellular Systems publication_identifier: isbn: - 978-3-99078-058-9 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '14274' relation: part_of_dissertation status: public status: public supervisor: - first_name: Michael K full_name: Sixt, Michael K id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 title: Adaptive strategies of dendritic cell migration in response to environmental cues type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19431' abstract: - lang: eng text: "Gene expression is crucial for cell differentiation, development and survival of\r\norganisms. It consists of several steps, starting with transcription that is mediated by\r\nRNA polymerases. These are protein machineries transcribing and producing different\r\ntypes of RNAs. Although, the individual steps of transcription by RNA polymerase II\r\n(Pol II) as well as the structure of Pol II has been extensively studied, surprisingly,\r\nthere is still little known about its regulation and assembly in cytoplasm. Among the\r\nproteins that are important in biogenesis of Pol II are RNA polymerase II associating\r\nproteins (RPAP) and small GPN-loop GTPases (GPN). Both of these protein groups\r\nwere shown to take essential part in assembly of Pol II.\r\nThe aim of this project was to deepen our knowledge in regulation of Pol II in\r\nthe cytoplasm as well as the proteins involved in this process. Techniques of structural\r\nbiology, biochemistry and cell biology were employed to study and characterize cytoplasmic Pol II and its interacting partners.\r\nThis study shows for the first time the structure of cytoplasmic Pol II at high\r\nresolution. The structure also reveals proteins interacting with Pol II in cytoplasm,\r\nnamely GDOWN1, RPAP2. Comparing the structure of cytoplasmic Pol II with transcribing Pol II revealed striking difference in clamp region that is not in closed state.\r\nFurthermore, GDOWN1 and RPAP2 make steric clashes with various transcription\r\nfactors bound to Pol II during different stages of transcription. Even though GPN1 and\r\nGPN3 proteins were not resolved in the cytoplasmic Pol II structure, they are part of\r\nthe complex and their interaction with Pol II was confirmed in vitro. RPAP2 stabilizes\r\nthese proteins on Pol II and several experiments suggest that they interact with the\r\nclamp region. In addition, GDOWN1, RPAP2 and GPNs might keep clamp in open or\r\npartially open state. Based on these results I propose a novel model of regulation of\r\nPol II in cytoplasm. GDOWN1, RPAP2, GPN1 and GPN3 bind to Pol II in cytoplasm\r\nand doing so they can prevent pre-mature binding of DNA or RNA and different transcription factors to Pol II in cytoplasm or before engaging in transcription nucleus.\r\nThis research contributes to the current knowledge of molecular mechanisms\r\nof Pol II regulation in cytoplasm." acknowledged_ssus: - _id: LifeSc - _id: EM-Fac - _id: ScienComp acknowledgement: 'I would also like to acknowledge the ISTA Facilities: Lab Support Facility, Protein Services and Electron Microscopy Facility (EMF) and Scientific Computing. EMF for their support during data collections and troubleshooting, especially Valentin. Scientific Computing for solving quickly any issues related with cluster.' alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Annamaria full_name: Hlavata, Annamaria id: 36062FEC-F248-11E8-B48F-1D18A9856A87 last_name: Hlavata citation: ama: Hlavata A. Regulation of Cytoplasmic RNA Polymerase II. 2025. doi:10.15479/10.15479/AT-ISTA-19431 apa: Hlavata, A. (2025). Regulation of Cytoplasmic RNA Polymerase II. Institute of Science and Technology Austria. https://doi.org/10.15479/10.15479/AT-ISTA-19431 chicago: Hlavata, Annamaria. “Regulation of Cytoplasmic RNA Polymerase II.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/10.15479/AT-ISTA-19431. ieee: A. Hlavata, “Regulation of Cytoplasmic RNA Polymerase II,” Institute of Science and Technology Austria, 2025. ista: Hlavata A. 2025. Regulation of Cytoplasmic RNA Polymerase II. Institute of Science and Technology Austria. mla: Hlavata, Annamaria. Regulation of Cytoplasmic RNA Polymerase II. Institute of Science and Technology Austria, 2025, doi:10.15479/10.15479/AT-ISTA-19431. short: A. Hlavata, Regulation of Cytoplasmic RNA Polymerase II, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-03-20T12:52:47Z date_published: 2025-03-20T00:00:00Z date_updated: 2026-04-07T11:46:32Z day: '20' ddc: - '572' degree_awarded: PhD department: - _id: GradSch - _id: CaBe doi: 10.15479/10.15479/AT-ISTA-19431 file: - access_level: closed checksum: b7ddf424ffe95f8c767c53c8bb62d4f3 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: ahlavata date_created: 2025-03-24T12:48:36Z date_updated: 2026-03-20T23:30:04Z embargo_to: open_access file_id: '19448' file_name: PhD_Thesis_Hlavata_final_submission.docx file_size: 23506747 relation: source_file - access_level: open_access checksum: 6c5a59c9bac467c3d0b3ffb8ea6d9fd4 content_type: application/pdf creator: ahlavata date_created: 2025-03-24T12:51:10Z date_updated: 2026-03-20T23:30:04Z embargo: 2026-03-20 file_id: '19449' file_name: PhD_Thesis_Hlavata_final_submission_update.pdf file_size: 9478591 relation: main_file file_date_updated: 2026-03-20T23:30:04Z fulldoi: https://doi.org/10.15479/10.15479/AT-ISTA-19431 has_accepted_license: '1' language: - iso: eng month: '03' oa: 1 oa_version: Published Version page: '83' publication_identifier: eissn: - 2663-337X isbn: - 978-3-99078-055-8 publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Carrie A full_name: Bernecky, Carrie A id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87 last_name: Bernecky orcid: 0000-0003-0893-7036 title: Regulation of Cytoplasmic RNA Polymerase II type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_type: closed access _id: '19302' abstract: - lang: eng text: "Social interaction networks of insect colonies facilitate efficient information exchange and\r\ndemonstrate adaptive changes to mitigate disease transmission. While circadian rhythms\r\ninfluence individual behaviour, their role in shaping colony-level defences against pathogens\r\nremains unexplored. Here, we investigate whether social networks of the black garden ant,\r\nLasius niger, exhibit circadian rhythms and how these rhythms influence disease vulnerability\r\nwhen colonies are exposed to a pathogen during the day or the night.\r\nWe first establish baseline daily variations in activity and network dynamics in pathogen-free\r\ncolonies, revealing constitutive daily fluctuations in disease susceptibility. Subsequently, we\r\nexamine pathogen-induced changes in sanitary care and network dynamics by exposing\r\nforagers to a natural pathogen (Metarhizium brunneum) during either the day or the night.\r\nIndividual pathogen loads were measured after a nine-hour post-exposure period to evaluate\r\ntransmission outcomes.\r\nOur results demonstrate that diurnal ant colonies maintain robust circadian patterns in network\r\nproperties while flexibly adapting to pathogen exposure. Ants upregulate sanitary care\r\nirrespective of exposure timing, prioritising the protection of the valuable colony centre\r\nconsisting of nurses and the queen. These findings underscore the robustness and adaptability\r\nof ant colonies in balancing circadian rhythms with effective social immune responses." acknowledged_ssus: - _id: LifeSc acknowledgement: Thank you to the Lab Support Facility at ISTA. Thank you to the European Research Council (ERC) for their funding under the European Union’s Horizon 2020 research and innovation program (ERC Consolidator Grant EPIDEMICSonCHIP, No. 771402, to Sylvia Cremer, and ERC Starting Grant DISEASE, No. 802628, to Nathalie Stroeymeyt). alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Linda full_name: Sartoris, Linda id: 2B9284CA-F248-11E8-B48F-1D18A9856A87 last_name: Sartoris citation: ama: Sartoris L. The effect of circadian rhythm on organisational immunity of ant colonies. 2025. doi:10.15479/AT-ISTA-19302 apa: Sartoris, L. (2025). The effect of circadian rhythm on organisational immunity of ant colonies. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19302 chicago: Sartoris, Linda. “The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19302. ieee: L. Sartoris, “The effect of circadian rhythm on organisational immunity of ant colonies,” Institute of Science and Technology Austria, 2025. ista: Sartoris L. 2025. The effect of circadian rhythm on organisational immunity of ant colonies. Institute of Science and Technology Austria. mla: Sartoris, Linda. The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19302. short: L. Sartoris, The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-03-06T12:16:54Z date_published: 2025-02-24T00:00:00Z date_updated: 2026-03-02T23:31:14Z day: '24' ddc: - '577' degree_awarded: PhD department: - _id: GradSch - _id: SyCr doi: 10.15479/AT-ISTA-19302 ec_funded: 1 file: - access_level: closed checksum: 7e9466dcf3681454211b74b5107e9f7b content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: lsartori date_created: 2025-03-07T10:16:11Z date_updated: 2026-02-23T23:30:03Z embargo_to: open_access file_id: '19310' file_name: Thesis_Linda_Sartoris.docx file_size: 7129583 relation: source_file - access_level: closed checksum: 2ccfcf32f0590bb0ec1a488e606a73f5 content_type: application/pdf creator: lsartori date_created: 2025-03-11T10:42:20Z date_updated: 2026-03-02T23:31:13Z description: for printing purposes only embargo_to: open_access file_id: '19384' file_name: thesis_Sartoris_for_print.pdf file_size: 3199703 relation: other - access_level: open_access checksum: 1d1f3c1279065b1a7f407ff6d1ee1503 content_type: application/pdf creator: lsartori date_created: 2025-03-11T10:52:00Z date_updated: 2026-02-23T23:30:03Z embargo: 2026-02-23 file_id: '19385' file_name: Thesis_Linda_Sartoris.pdf file_size: 3183186 relation: main_file file_date_updated: 2026-03-02T23:31:13Z fulldoi: https://doi.org/10.15479/AT-ISTA-19302 has_accepted_license: '1' language: - iso: eng month: '02' oa: 1 oa_version: Published Version page: '85' project: - _id: 2649B4DE-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '771402' name: Epidemics in ant societies on a chip publication_identifier: eissn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Sylvia full_name: Cremer, Sylvia id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87 last_name: Cremer orcid: 0000-0002-2193-3868 title: The effect of circadian rhythm on organisational immunity of ant colonies tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_embargo: 6 months OA_place: publisher _id: '19456' abstract: - lang: eng text: "Making decisions requires flexibly adapting to changing environments, a process that\r\ndepends on accurately interpreting current contingencies and integrating them with\r\npast experience. Two brain regions are particularly critical for this process, the medial\r\nprefrontal cortex (mPFC) and the hippocampus. Using contextual information from the\r\nhippocampus, the mPFC selects relevant cognitive frameworks and suppresses\r\nirrelevant ones to guide appropriate actions. Several studies have shown that some\r\nmPFC pyramidal neurons become spatially tuned when spatial information is required\r\nto guide goal-directed behavior. However, the role of prefrontal spatial representations\r\nin learning and decision making is not well understood. This work aims to characterize\r\nthe role of mPFC spatial tuning in supporting a contextual association task. Rats were\r\ntrained to learn two cue–location associations on a radial arm maze over multiple days,\r\nwhile we simultaneously recorded from dorsal CA1 of the hippocampus and the\r\nprelimbic area of the mPFC. We describe a subset of spatially tuned hippocampal and\r\nprefrontal pyramidal neurons that “flicker” between multiple spatial representations on\r\ndifferent trials, suggesting dynamic, context-dependent coding. This flickering may\r\nprovide a substrate for how the network reorganizes in response to task demands,\r\nlikely by enabling the flexible evaluation of competing representations. " acknowledged_ssus: - _id: PreCl - _id: Bio - _id: LifeSc - _id: M-Shop alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Andrea D full_name: Cumpelik, Andrea D id: 3F158B32-F248-11E8-B48F-1D18A9856A87 last_name: Cumpelik orcid: 0000-0003-1727-6612 citation: ama: Cumpelik AD. The role of prefrontal spatial coding in supporting a contextual association task. 2025. doi:10.15479/AT-ISTA-19456 apa: Cumpelik, A. D. (2025). The role of prefrontal spatial coding in supporting a contextual association task. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19456 chicago: Cumpelik, Andrea D. “The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19456. ieee: A. D. Cumpelik, “The role of prefrontal spatial coding in supporting a contextual association task,” Institute of Science and Technology Austria, 2025. ista: Cumpelik AD. 2025. The role of prefrontal spatial coding in supporting a contextual association task. Institute of Science and Technology Austria. mla: Cumpelik, Andrea D. The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19456. short: A.D. Cumpelik, The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-03-25T11:22:38Z date_published: 2025-02-18T00:00:00Z date_updated: 2026-04-07T12:37:58Z day: '18' ddc: - '612' degree_awarded: PhD department: - _id: GradSch - _id: JoCs doi: 10.15479/AT-ISTA-19456 file: - access_level: open_access checksum: 1c7573303d8e5f6da3eb03d59055390f content_type: application/pdf creator: acumpeli date_created: 2025-03-25T11:07:55Z date_updated: 2025-09-30T22:30:02Z embargo: 2025-09-30 file_id: '19457' file_name: 2025_Thesis_Cumpelik_corrections_PDFA.pdf file_size: 11869040 relation: main_file - access_level: closed checksum: b93265ebd9a53f7a14100d0d48b4ff5b content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: acumpeli date_created: 2025-03-25T11:08:05Z date_updated: 2025-09-30T22:30:02Z embargo_to: open_access file_id: '19458' file_name: 2025_Thesis_Cumpelik_corrections.docx file_size: 20436467 relation: source_file file_date_updated: 2025-09-30T22:30:02Z fulldoi: https://doi.org/10.15479/AT-ISTA-19456 has_accepted_license: '1' keyword: - neuroscience - decision making - learning - cognitive flexibility - medial prefrontal cortex - hippocampus - electrophysiology language: - iso: eng month: '02' oa: 1 oa_version: Published Version page: '96' publication_identifier: isbn: - 978-3-99078-056-5 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Jozsef L full_name: Csicsvari, Jozsef L id: 3FA14672-F248-11E8-B48F-1D18A9856A87 last_name: Csicsvari orcid: 0000-0002-5193-4036 title: The role of prefrontal spatial coding in supporting a contextual association task type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19993' abstract: - lang: eng text: "Ants are frequently challenged by different pathogens, which they counter with\r\nindividual and collective responses. Usually, the pathogens like fungi or viruses are\r\nsolitary and passive pathogens transmitted from host to host. Here, we use a nematobacterial pathogen complex to study worm-borne disease in black garden ants. These\r\nentomopathogenic nematodes are active parasites with an own behavior and chasing\r\npray.\r\nIn the first chapter, we investigated the basic biology of the host-pathogen relationship.\r\nWe tested different ant life stages and found that adult ants display defense behaviors\r\nand are generally resistant to nematode infection, whereas brood is highly susceptible.\r\nIn the case of worker pupae, we found a slight protective effect of the cocoon. When\r\nlarvae are accompanied by adults, meaning a queen or a group of workers, survival is\r\nsignificantly enhanced. Moreover, we found that nematodes can transmit from infected\r\ncadavers to healthy worker larvae, confirming a transmissible disease in ants. Again,\r\nworker presence significantly reduces transmission risk. In the end, we were also able\r\nto disentangle the pathogen system and investigate the pathogenic effect of the\r\nbacterial and nematode components.\r\nIn the second chapter, we studied the effect of multiple infections in adult queens and\r\nqueen larvae. By multiple exposures in the mode of coinfection and superinfections,\r\nwe wanted to assess the detrimental effect of combined fungal and nematode\r\nexposure to better understand how the pathogens interact with each other in an ant\r\nhost. We found instances where combined exposure lead to higher mortality in a given\r\ntime frame in both, adult queens and queen larvae.\r\nOverall entomopathogenic nematodes are a promising model to study worm infections\r\nin ants which extend our knowledge on collective disease defense." acknowledged_ssus: - _id: LifeSc alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Florian full_name: Strahodinsky, Florian id: 979E35EE-C996-11E9-8C7C-CF13E6697425 last_name: Strahodinsky citation: ama: Strahodinsky F. Social immunity in a tri-partite host-pathogen relationship. 2025. doi:10.15479/AT-ISTA-19993 apa: Strahodinsky, F. (2025). Social immunity in a tri-partite host-pathogen relationship. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19993 chicago: Strahodinsky, Florian. “Social Immunity in a Tri-Partite Host-Pathogen Relationship.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19993. ieee: F. Strahodinsky, “Social immunity in a tri-partite host-pathogen relationship,” Institute of Science and Technology Austria, 2025. ista: Strahodinsky F. 2025. Social immunity in a tri-partite host-pathogen relationship. Institute of Science and Technology Austria. mla: Strahodinsky, Florian. Social Immunity in a Tri-Partite Host-Pathogen Relationship. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19993. short: F. Strahodinsky, Social Immunity in a Tri-Partite Host-Pathogen Relationship, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-07-10T14:12:20Z date_published: 2025-07-11T00:00:00Z date_updated: 2026-04-07T12:39:58Z day: '11' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: SyCr doi: 10.15479/AT-ISTA-19993 file: - access_level: closed checksum: df3a02f0d937ea9a3d79d5fb94fff097 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: fstrahod date_created: 2025-07-14T13:18:37Z date_updated: 2026-01-15T23:30:03Z embargo_to: open_access file_id: '20021' file_name: Thesis_Florian_Strahodinsky_DOCX.docx file_size: 9857392 relation: source_file - access_level: open_access checksum: 7164c21fe1946e839f7b8acd255ce803 content_type: application/pdf creator: fstrahod date_created: 2025-07-14T13:18:38Z date_updated: 2026-01-15T23:30:03Z embargo: 2026-01-15 file_id: '20022' file_name: Thesis_Florian_Strahodinsky_PDF.pdf file_size: 6439602 relation: main_file file_date_updated: 2026-01-15T23:30:03Z fulldoi: https://doi.org/10.15479/AT-ISTA-19993 has_accepted_license: '1' language: - iso: eng month: '07' oa: 1 oa_version: Published Version page: '138' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Sylvia full_name: Cremer, Sylvia id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87 last_name: Cremer orcid: 0000-0002-2193-3868 title: Social immunity in a tri-partite host-pathogen relationship tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ...