@article{21948,
  abstract     = {The cerebral cortex comprises diverse neuron and glial cell types generated by radial glial progenitors (RGPs) during development. Although RGPs broadly differentiate according to temporally and spatially regulated molecular logics, the lineage hierarchies linking individual progenitors to defined cell (sub)types are not well understood. Clone-resolved transcriptomics, combining molecular barcoding and single-cell RNA sequencing, allow high-resolution lineage tracing at the single-clone/cell level across different species and models. In this mini-review, we synthesize recent advances in this field, uncovering unexpected lineage relationships in the developing brain, with a particular focus on the cerebral cortex. We further highlight new insights into species-specific differences in the developmental programs generating cell-type diversity, linking changes in clonal architecture to lineage diversification during cortical evolution.},
  author       = {Varela Martínez, Irene and Pipicelli, Fabrizia and Hippenmeyer, Simon},
  issn         = {1879-0380},
  journal      = {Current Opinion in Genetics & Development},
  publisher    = {Elsevier},
  title        = {{Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics}},
  doi          = {10.1016/j.gde.2026.102487},
  volume       = {99},
  year         = {2026},
}

@article{21039,
  abstract     = {Cellular plasticity, the ability of a differentiated cell to adopt another phenotypic identity, is restricted under basal conditions, but can be elicited upon damage. However, the molecular mechanism enabling such plasticity remains largely unexplored. Here, we report damage-induced cellular plasticity of secretory enteroendocrine cells (EEs) in the adult Drosophila midgut. Ionizing radiation induces EE fate conversion and activates stress-responsive programs in EE lineages, accompanied by the induction of the stress-inducible transcription factor Xrp1 and the cytokine gene upd3. Xrp1 and upd3 are both necessary for radiation-induced EE plasticity. Under basal conditions, EE-specific Xrp1 overexpression triggers ectopic expression of progenitor-specific genes, which is necessary for Xrp1 to drive EE plasticity. Our work identifies Xrp1 as a crucial regulator that coordinates damage-induced signaling and transcriptional reprogramming, enabling the reactivation of cellular plasticity in differentiated cells.},
  author       = {Qian, Qingyin and Nagai, Hiroki and Sanaki, Yuya and Hayashi, Makoto and Kimura, Kenichi and Nakajima, Yu Ichiro and Niwa, Ryusuke},
  issn         = {1477-9129},
  journal      = {Development},
  number       = {2},
  publisher    = {Company of Biologists},
  title        = {{Xrp1 drives damage-induced cellular plasticity of enteroendocrine cells in the adult Drosophila midgut}},
  doi          = {10.1242/dev.205225},
  volume       = {153},
  year         = {2026},
}

@article{22148,
  abstract     = {How the twin-arginine translocase (Tat) system transports fully folded substrate proteins across cellular membranes without disrupting membrane integrity has been a fundamental question in cell biology for decades. The Tat system, found in prokaryotes and plant organelles, recognizes a cargo signal peptide via a conserved twin-arginine motif. The multi-subunit Tat complex facilitates the proton-motive-force-dependent translocation process, yet its overall architecture has remained unknown. Here, we present the cryo-electron microscopy (cryo-EM) structure of the Escherichia coli (E. coli) trimeric TatB₃C₃ complex with bound substrate SufI, assembled in vivo. The complex adopts an unusual, wide-open, bowl-shaped architecture with a polar inner cavity. Unexpectedly, the cargo is engaged in a dual-contact mode: while the signal peptide binds inside one TatBC unit, the folded domain docks tightly onto an adjacent unit, possibly performing a proofreading function. This structure provides a mechanistic framework for substrate engagement and suggests the direct involvement of the entire Tat complex in substrate translocation.},
  author       = {Zhao, Ziyu and Sazanov, Leonid A},
  issn         = {1097-4164},
  journal      = {Molecular Cell},
  publisher    = {Elsevier},
  title        = {{Structure of E. Coli twin-arginine translocase (Tat) complex with bound cargo}},
  doi          = {10.1016/j.molcel.2026.05.026},
  year         = {2026},
}

@misc{22189,
  abstract     = {Raw images for SDS and Blue Native gels, western blots and spot growth assays, related to figures S1 and S2.},
  author       = {Sazanov, Leonid A},
  keywords     = {Protein Purification},
  publisher    = {Mendeley Data},
  title        = {{Structure of E. coli twin-arginine translocase (Tat) complex with bound cargo. Zhao et al.}},
  doi          = {10.17632/V2G3P9N985.1},
  year         = {2026},
}

@misc{22704,
  abstract     = {The medial habenula (MHb) is implicated in regulating emotional responses to aversive events. Studies in zebrafish have identified a remarkable morphological left–right asymmetry in the dorsal habenula (zebrafish equivalent of mammalian MHb)-interpeduncular nucleus (IPN) pathway and its asymmetrical roles in behavior. However, there is little evidence for structural or functional lateralization in the mammalian MHb-IPN pathway. Here, we investigated the synaptic properties of left- and right-MHb afferents to the IPN and their roles in the expression of conditioned fear in mice. We found that each IPN neuron receives inputs from both left and right MHb, but the left MHb-originating synapses exhibit lower release probability and higher γ-aminobutyric acid type B receptor (GABABR)-mediated potentiation compared to the right MHb-originating synapses. Interestingly, these asymmetrical properties persist in the inversus visceral mutant mice with normal internal organ laterality (situs solitus), but nearly disappear in those with reversed internal organ laterality (situs inversus). Behaviorally, chemogenetic inhibition of cholinergic neurons and conditional deletion of GABABR in the left, but not the right, MHb significantly attenuated cue-dependent fear recall. Our results demonstrate functional asymmetry of the MHb under partial influence of the nodal flow in mice, revealing a predominant role of GABABR-mediated signaling in the left MHb-IPN pathway in modulating fear memories. These findings suggest that lateralized MHb pathways could represent a fundamental principle in the neural regulation of emotion across species but that they develop differently in zebrafish and mice.
},
  author       = {Le Monnier, Elodie and Önal, Cihan},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Asymmetrical modulation of fear expression via GABAB receptors in the mouse medial habenula}},
  doi          = {10.15479/AT-ISTA-22704},
  year         = {2026},
}

@inproceedings{22717,
  abstract     = {We study concurrent graph games where n players cooperate against an opponent to reach a set of target states. Unlike traditional settings, we study distributed randomisation: team players do not share a source of randomness, and their private random sources are hidden from the opponent and from each other.

We show that memoryless strategies are sufficient for the threshold problem (deciding whether there is a strategy for the team that ensures winning with probability that exceeds a threshold), a result that not only places the problem in the Existential Theory of the Reals (ER) but also enables the construction of value iteration algorithms. We additionally show that the threshold problem is NP-hard. For the almost-sure reachability problem, we prove NP-completeness.

We introduce Individually Randomised Alternating-time Temporal Logic (IRATL). This logic extends the standard ATL framework to reason about probability thresholds, with semantics explicitly designed for coalitions that lack a shared source of randomness. On the practical side, we implement and evaluate a solver for the threshold and almost-sure problem based on the algorithms that we develop.},
  author       = {Brice, Leonard J and Henzinger, Thomas A and Montaseri, Alipasha and Shafiee, Ali and Thejaswini, K. S.},
  booktitle    = {38th International Conference on Computer Aided Verification},
  isbn         = {9783032325181},
  issn         = {1611-3349},
  location     = {Lisbon, Portugal},
  pages        = {215--236},
  publisher    = {Springer Nature},
  title        = {{Randomise alone, reach as a team}},
  doi          = {10.1007/978-3-032-32519-8_12},
  volume       = {16682},
  year         = {2026},
}

@inproceedings{22722,
  abstract     = {The local structure of a protein strongly impacts its function and interactions
with other molecules. Representing local biomolecular environments remains a
key challenge while applying machine learning approaches over protein structures. The structural and chemical variability of these environments makes them
challenging to model, and performing representation learning on these objects
remains largely under-explored. In this work, we propose representations for
local protein environments that leverage intermediate features from machine learning force fields (MLFFs). We extensively benchmark state-of-the-art MLFFs,
comparing their performance across latent spaces and downstream tasks, and
show that their embeddings capture local structural (e.g., secondary motifs) and
chemical features (e.g., amino acid identity and protonation state), organizing
protein environments into a structured manifold. We show that these representations enable zero-shot generalization and transfer across diverse downstream
tasks. As a case study, we build a physics-informed, uncertainty-aware chemical shift predictor that achieves state-of-the-art accuracy in biomolecular NMR
spectroscopy. Our results establish MLFFs as general-purpose, reusable representation learners for protein modeling, opening new directions in representation learning for structured physical systems. Code and data are available at
https://github.com/mb012/MLFF_representation.
},
  author       = {Bojan, Meital I and Vedula, Sanketh and Maddipatla, Sai A and Sellam, Nadav E and Rzayev, Anar and Napoli, Federico and Schanda, Paul and Bronstein, Alexander},
  booktitle    = {14th International Conference on Learning Representations},
  location     = {Rio de Janeiro, Brazil},
  pages        = {100760--100799},
  title        = {{Representing local protein environments with machine learning force fields}},
  volume       = {2026},
  year         = {2026},
}

@inproceedings{22719,
  abstract     = {We study the problem of generating paths on a graph that satisfy a collection of w-regular objectives. We propose a decoupled framework in which each objective is assigned to an independent agent that selects a local policy, while a scheduler—oblivious to the graph and objective—dynamically composes these policies into a single path. We ask when such a composition satisfies all objectives, assuming their conjunction is realizable. The framework enables modular policy design but raises fundamental compositional challenges. We show that even extremely fair deterministic schedulers do not ensure correctness, and that stochastic schedulers, while necessary, are insufficient without coordination. For safety objectives, we demonstrate that fully decentralized implementations are impossible, and we introduce a protocol for synchronizing on maximal safe actions. For non-safety objectives, we introduce conventions—simple, a priori restrictions agreed upon before the graph or objectives are revealed—that guarantee satisfaction of all objectives when followed by all agents. We characterize minimally restrictive conventions for major subclasses of w-regular objectives. In particular, Büchi objectives admit universal composition of finite-memory policies without scheduler communication; co-Büchi objectives require only knowledge of whether the agent was scheduled; and parity objectives additionally require knowledge of which agent was scheduled.},
  author       = {Avni, Guy and Henzinger, Thomas A and Mallik, Kaushik and Sadhukhan, Suman and Thejaswini, K. S.},
  booktitle    = {38th International Conference on Computer Aided Verification},
  isbn         = {9783032325181},
  issn         = {1611-3349},
  location     = {Lisbon, Portugal},
  pages        = {237--257},
  publisher    = {Springer Nature},
  title        = {{Decoupled planning for multiple omega-regular objectives}},
  doi          = {10.1007/978-3-032-32519-8_13},
  volume       = {16682},
  year         = {2026},
}

@article{22713,
  abstract     = {Ground-based gravitational-wave (GW) observatories have detected approximately 200 binary black hole (BH) mergers. The astrophysical origin of these events is debated, with evidence suggesting that at least a subset originated from dynamic environments characterized by frequent close encounters. Accretion disks in active galactic nuclei (AGNs) are of particular interest, as certain observed features could be more readily produced within such environments. In this paper, we investigate the expected properties of mergers in these environments, and their dependence on various parameters, using 1D N-body simulations combined with a comprehensive semianalytical model. In our fiducial model, the distributions of masses (m1 and m2) and mass ratios (q ≡ m2/m1 ≤ 1) are similar to those observed. However, they depend strongly on the lifetime and density of the AGN disk and on the number and accretion efficiency of BHs, with higher masses predicted as these quantities increase. The most massive mergers, such as GW231123, can be produced either by efficient gas accretion or by hierarchical mergers among ≥3 generations of BHs. The observed negative correlation between q and the average effective spin (χeff), along with the positive correlation between χeff and the chirp mass (Mchirp), can be explained by a combination of efficient gas accretion, which promotes spin alignment, and hierarchical mergers, which produce high-∣χeff∣ and low-q binaries. Hierarchical mergers can also explain the negative correlation between q and the dispersion of χeff, as well as the positive correlation between ∣χeff∣and Mchirp. We present a comprehensive study on how the expected distribution of each of these quantities depends on model parameters and assumptions, which will aid the interpretation of observed GW population properties.},
  author       = {Tagawa, Hiromichi and Haiman, Zoltán and Kocsis, Bence},
  issn         = {1538-4357},
  journal      = {The Astrophysical Journal},
  number       = {1},
  publisher    = {IOP Publishing},
  title        = {{Properties of black hole mergers in disks of active galactic nuclei}},
  doi          = {10.3847/1538-4357/ae8760},
  volume       = {1007},
  year         = {2026},
}

@article{22720,
  abstract     = {Circadian clocks underlie daily rhythms in physiology by coordinating temporal patterns of gene expression and protein function throughout the body. At the core of this system in mammals is CLOCK/BMAL1, a ubiquitously expressed heterodimeric transcription factor complex that orchestrates tissue-specific circadian gene expression. The basis for this specificity remains unclear, but tissue-specific interactions at chromatin could provide one. Here we used chromatin immunoprecipitation coupled to mass spectrometry to map CLOCK/BMAL1-associated protein complexes on chromatin in mouse liver, kidney and lung. We detected 1,510 associated proteins, most of which were tissue-specific and not explained by protein abundance. Among these, we identified the homeodomain transcription factors PROX1, HNF1B and HOXA5 as tissue-enriched interactors that bind BMAL1, co-occupy most BMAL1 genomic sites and establish organ-restricted circadian transcription. Our findings demonstrate that tissue-specific transcription factors confer cellular identity on the core clock, thereby contributing to organ-specific patterns of rhythmic gene expression.},
  author       = {Aygenli, Fatih and Huschet, Lukas A. and Popp, Tanja and Ribeiro, Andrea and Barkhatova, Darina and Jouffe, Céline and Trozzo, Ricardo and Menet, Jerome S. and Rad, Roland and Dyar, Kenneth A. and Lech, Maciej and Straub, Tobias and Michael, Alicia and Robles, Maria S.},
  issn         = {1476-4679},
  journal      = {Nature Cell Biology},
  publisher    = {Springer Nature},
  title        = {{CLOCK/BMAL1 interactome uncovers homeodomain factors as tissue regulators}},
  doi          = {10.1038/s41556-026-02041-4},
  year         = {2026},
}

@article{22718,
  abstract     = {Both Newtonian and non-Newtonian fluids may exhibit complex slip behaviour at the boundary. We examine a broad class of slip boundary conditions that generalises the commonly used Navier slip, perfect slip, stick-slip and Tresca friction boundary conditions. In particular, set-valued, nonmonotone, noncoercive and dynamic relations may occur. For a unifying framework of such relations, we present a fully discrete numerical scheme for the time-dependent Navier–Stokes equations subject to impermeability and general slip-type boundary conditions on polyhedral domains. Based on compactness arguments, we prove convergence of subsequences, finally ensuring the existence of a weak solution. The numerical scheme uses a general inf-sup stable pair of finite element spaces for the velocity and pressure, a regularisation approach for the implicit slip boundary condition and, most importantly, a general Nitsche method to impose the impermeability and a backward Euler time stepping. One of the key tools in the convergence proof is an inhomogeneous Korn inequality that includes a normal trace term.},
  author       = {Gazca-Orozco, Pablo Alexei and Gmeineder, Franz and Maringová, Erika and Tscherpel, Tabea},
  issn         = {1793-6314},
  journal      = {Mathematical Models and Methods in Applied Sciences},
  publisher    = {World Scientific Publishing},
  title        = {{A Nitsche method for incompressible fluids with general dynamic boundary conditions}},
  doi          = {10.1142/S0218202526500508},
  year         = {2026},
}

@article{22734,
  abstract     = {Silver selenide (Ag2Se) is a promising near-room-temperature thermoelectric material, but its narrow stoichiometric window and β–α phase transition complicate reproducible microstructure control. Here, we present a mismatch-assisted microstructure engineering strategy in which Ag2Se particles are treated with polyanionic ZnSe complexes and consolidated through the β–α transition to introduce ZnSe nanoprecipitates, Ag2Se/ZnSe interfaces, and local strain fields. The crystallographic mismatch between ZnSe and Ag2Se, together with the Zn2+/Ag+ size difference, amplifies phase-transition-induced deformation and promotes high-density dislocations with periodic strain modulations. This defect architecture suppresses grain coarsening, removes excess Ag, limits Ag-interstitial formation, and reduces lattice thermal conductivity through lattice softening and multiscale phonon scattering. Ag2Se–4%ZnSe nanocomposites achieve a peak zTmax of 1.13 at 369 K and a zTavg of 1.08 from 300 to 380 K, demonstrating mismatch-driven defect engineering through the β–α phase transition as a route for optimizing Ag2Se-based thermoelectrics.},
  author       = {Liu, Yu and Kleinhanns, Tobias and Spadaro, Maria Chiara and Genç, Aziz and Horta, Sharona and Navita, Navita and Costanzo, Tommaso and Dutkiewicz, Ewelina and Arbiol, Jordi and Hong, Min and Ibáñez, Maria},
  issn         = {2380-8195},
  journal      = {ACS Energy Letters},
  number       = {8},
  pages        = {5752--5762},
  publisher    = {American Chemical Society},
  title        = {{Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se}},
  doi          = {10.1021/acsenergylett.6c01499},
  volume       = {11},
  year         = {2026},
}

@article{22715,
  abstract     = {Grazing-incidence small-angle scattering (GISAS) is a relatively young technique with important applications in thin-film technology and untapped potential when it comes to 2D analysis on an absolute intensity scale. Approaching standardization and reference methods early is foundational for reproducibility and comparability across laboratories and reduction of systematic error sources. It underpins trust in data obtained and accelerates innovation by ensuring that scientists work from a common methodological baseline. Accordingly, obtaining reproducible results from different GISAS instruments requires an agreement on how measurements are performed, instruments calibrated and terms defined. To pave the way for standardization and reference methods, we surveyed GISAS practitioners on what comes before an experiment: hardware, software, sample alignment and instrument calibration. Twenty-two questions were designed to elucidate the state of the art, which can be used for the development of reference methods. Our data on 27 instruments provide the basis for standardization. With very few exceptions, we found laboratories prepared to implement future reference methods, but no consensus emerges naturally for sample alignment and instrument calibration. We, that is the GISAS community, are thus in a position to embark on the journey of standardization.},
  author       = {Hörmann, Anja F. and Balazs, Daniel and Breßler, Ingo and Klokic, Sumea and Moradi, Melika and Solano, Eduardo and Stellhorn, Annika and Pauw, Brian R.},
  issn         = {1600-5767},
  journal      = {Journal of Applied Crystallography},
  keywords     = {grazing incidence, reference methods, calibration, standardization, community},
  number       = {4},
  pages        = {1247--1253},
  publisher    = {International Union of Crystallography},
  title        = {{Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration}},
  doi          = {10.1107/S1600576726005741},
  volume       = {59},
  year         = {2026},
}

@article{22716,
  abstract     = {We give an algorithm that, with high probability, maintains a (1-ε)-approximate s-t maximum flow in undirected, uncapacitated n-vertex graphs undergoing m edge insertions in Õ(m+ n F^*/ε) total update time, where F^{*} is the maximum flow on the final graph. This is the first algorithm to achieve polylogarithmic amortized update time for dense graphs (m = Ω(n²)), and more generally, for graphs where F^* = Õ(m/n). At the heart of our incremental algorithm is the residual graph sparsification technique of Karger and Levine [SICOMP '15], originally designed for computing exact maximum flows in the static setting. Our main contributions are (i) showing how to maintain such sparsifiers for approximate maximum flows in the incremental setting and (ii) generalizing the cut sparsification framework of Fung et al. [SICOMP '19] from undirected graphs to balanced directed graphs.},
  author       = {Goranci, Gramoz and Henzinger, Monika H and Räcke, Harald and Sricharan, A. R.},
  issn         = {1549-6333},
  journal      = {ACM Transactions on Algorithms},
  number       = {3},
  publisher    = {ACM},
  title        = {{Incremental approximate maximum flow via residual graph sparsification}},
  doi          = {10.1145/3816252},
  volume       = {22},
  year         = {2026},
}

@article{22714,
  abstract     = {Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the cerebral cortex through incompletely understood processes. We combined Mosaic Analysis with Double Markers at embryonic stages (E)12.5 and E13.5 with early postnatal callosal tracing to dissect RGP lineage progression. We find that multipotent RGPs generate all extra-telencephalic (ET) and intra-telencephalic (IT) PNs via parallel sublineages that emerge simultaneously at neurogenesis onset. ET-PN production progresses exclusively via small, self-consuming lineages; IT-PN lineages feature RGPs generating large translaminar outputs. The early emergence of IT-PN–fated RGPs, coinciding with a switch to direct neurogenesis, contributes to the stereotyped population-level progression of the multipotent lineage. We also identify POU3F transcription factors as candidate regulators of IT-PN fate via noncanonical mitotic chromatin binding. The results support a model whereby IT- and ET-PNs arise from an early bifurcation and parallel specification within the multipotent RGP lineage.},
  author       = {Varela Martínez, Irene and Villalba Requena, Ana and García-Marqués, Jorge and Aguilera, Alfonso and Castro, Diogo S. and Hippenmeyer, Simon and Nieto, Marta},
  issn         = {2375-2548},
  journal      = {Science Advances},
  number       = {32},
  pages        = {eadw5487},
  publisher    = {AAAS},
  title        = {{Early fate diversification of radial glial progenitors during corticogenesis}},
  doi          = {10.1126/sciadv.adw5487},
  volume       = {12},
  year         = {2026},
}

@article{22712,
  abstract     = {Ginseng (Panax ginseng) derives its renowned therapeutic properties from ginsenoside metabolites. However, the long cultivation cycle and susceptibility to diseases hinder the advancement of the ginseng industry. Here, we demonstrate that the embryonic protoderm of ginseng can efficiently produce ginsenosides. Single-cell transcriptome and mass spectrometry imaging analyses reveal that ginsenosides accumulate in the protoderm of ginseng embryonic callus (EC) at levels comparable to those in forest ginseng. Epigenetic analyses indicate that elevated histone acetylation and enhanced chromatin accessibility at regeneration- and ginsenoside metabolism-related gene loci are associated with the ginsenoside-producing capacity of EC. Increasing histone acetylation levels or overexpressing the regeneration-related WUSCHEL-RELATED HOMEOBOX11 (WOX11) gene further enhances ginsenoside production in EC. Our findings suggest that the protoderm of EC could serve as an in situ biological compartment for high-efficiency ginsenoside producion, offering a complementary approach to traditional ginseng cultivation.},
  author       = {Liu, Juan and Zhai, Ning and Zhang, Shiyi and Tamada, Yosuke and Li, Tonghui and Zhang, Linfan and Chen, Tong and Wang, Chenglin and Yang, Jian and Gao, Jiaqi and Li, Xiang and Zhou, Junhui and Zhang, Yonghong and Liu, Yu and Wang, Yuan and Friml, Jiří and Benková, Eva and Li, Chen and Xu, Lin and Huang, Luqi},
  issn         = {2041-1723},
  journal      = {Nature Communications},
  publisher    = {Springer Nature},
  title        = {{Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production}},
  doi          = {10.1038/s41467-026-74881-5},
  volume       = {17},
  year         = {2026},
}

@article{22711,
  abstract     = {When charge flows through a molecular circuit, it induces a magnetic field that allows the circuit to behave as a nanoscale electromagnet. However, in single-molecule circuits this magnetic field is usually weak. Here we show that radially π-conjugated carbon structures can support amplified circulating currents that generate local magnetic fields. Within tight-binding and density functional theory (DFT) frameworks, we first study cycloparaphenylene (CPP) junctions where both electrodes are attached to the same phenylene unit on the nanohoop. We observe an energy-dependent ring current component that traverses the whole macrocycle by mapping the local current density. Importantly, we find that destructive interference near degenerate resonances can reverse the ring current direction and amplify it strongly relative to the source–drain current. We show that this interference-driven design principle is general, and also carries over to C60 junctions. In fullerene, lower-lying degenerate resonances are more easily accessible through electrostatic gating, reaching a magnetic field of 14.2 mT under a 100 mV source–drain bias. This work thus provides new insights into ring currents in radially π-conjugated carbon structures and highlights their potential as design platforms for single-molecule electromagnets.},
  author       = {Shi, Wanzhuo and Korytár, Richard and Evers, Ferdinand and Tovar, John D. and Venkataraman, Latha},
  issn         = {2041-1723},
  journal      = {Nature Communications},
  publisher    = {Springer Nature},
  title        = {{Designing effective single-molecule electromagnets with radially π-conjugated carbon structures}},
  doi          = {10.1038/s41467-026-74365-6},
  volume       = {17},
  year         = {2026},
}

@misc{22687,
  abstract     = {Understanding enzyme function requires characterizing not only static structure but also dynamics and ligand interactions. NMR spectroscopy provides this insight at atomic resolution, yet for large proteins the difficulty of resonance assignment has largely confined such studies to systems below ∼50 kDa, or to observing only methyl groups. Here we present an integrated magic-angle spinning (MAS) and solution NMR study of the 134 kDa tetrameric malate dehydrogenase from Ignicoccus islandicus (IiMDH), an enzyme of particular interest as an evolutionary intermediate between allosteric lactate
dehydrogenases and non-allosteric malate dehydrogenases. By combining high-dimensional (up to 4D) MAS NMR experiments on sedimented protein with solution NMR, we achieved 92% backbone heavy- atom assignment and 91% assignment of all Ile-δ1, Leu-δ1/-δ2, Val-γ1/-γ2, Met-ε and Thr-γ methyl groups. Building on these assignments, we use various probes of backbone and sidechain dynamics: elevated MAS NMR 15N rotating-frame relaxation (R1ρ) points to microsecond motions in functionally critical regions, including the catalytic loop and the mobile surface loop. Complementary methyl-axis order parameters from solution NMR identified additional flexible sites in the hydrophobic core. Chemical shift perturbation experiments upon addition of the substrate analogue oxamate, monitored via backbone 1H-15N TROSY, revealed both active-site contacts and responses in helices α2F and α3G, regions implicated in allosteric signal transmission. The integrated approach demonstrated here exploits the distinct strengths of MAS and solution NMR, and provides a comprehensive view of structure, dynamics, and substrate interactions in a large oligomeric enzyme that would not be accessible by either technique alone.},
  author       = {Schanda, Paul and Napoli, Federico},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Data and scripts for: "Integrated solid/solution NMR assignment allows mapping dynamics and ligand binding in a 134 kDa enzyme"}},
  doi          = {10.15479/AT-ISTA-22687},
  year         = {2026},
}

@article{21746,
  abstract     = {As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution.},
  author       = {Vijatovic, David and Toma, Florina Alexandra  and Ignatyev, Y and Harrington, Zoe P and Sommer, Christoph M and Hauschild, Robert and Smits, Matthijs Geert and Dalla Vecchia, Marco and Trevisan, Alexandra J. and Chapman, Phillip and Julseth, Mara and Brenner-Morton, Susan and Gabitto, Mariano I. and Dasen, Jeremy S. and Bikoff, Jay B. and Sweeney, Lora Beatrice Jaeger},
  issn         = {2211-1247},
  journal      = {Cell Reports},
  number       = {4},
  publisher    = {Elsevier},
  title        = {{Multifold increase in spinal inhibitory cell types with emergence of limb movement}},
  doi          = {10.1016/j.celrep.2026.117227},
  volume       = {45},
  year         = {2026},
}

@phdthesis{22667,
  author       = {Vijatovic, David},
  isbn         = {978-3-99078-082-4},
  issn         = {2663-337X},
  pages        = {172},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Dissecting the molecular and functional basis of motor control in the frog Xenopus laevis}},
  doi          = {10.15479/AT-ISTA-22667},
  year         = {2026},
}