[{"OA_type":"hybrid","page":"1468-1480.e6","publisher":"Elsevier","day":"23","article_type":"original","_id":"21490","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","issue":"6","citation":{"ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023"},"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development."}],"PlanS_conform":"1","date_updated":"2026-03-24T08:36:40Z","volume":36,"type":"journal_article","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Identification of a novel regulator in auxin canalization","grant_number":"E271","_id":"bd906599-d553-11ed-ba76-abf8547645d7"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"date_published":"2026-03-23T00:00:00Z","corr_author":"1","department":[{"_id":"JiFr"}],"publication_status":"published","intvolume":" 36","publication_identifier":{"issn":["0960-9822"]},"author":[{"id":"01f96916-0235-11eb-9379-a323192643b7","full_name":"Li, Mingyue","first_name":"Mingyue","last_name":"Li"},{"full_name":"Rydza, Nikola","last_name":"Rydza","first_name":"Nikola"},{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","last_name":"Molnar","first_name":"Gergely"},{"full_name":"Nodzyński, Tomasz","last_name":"Nodzyński","first_name":"Tomasz"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"date_created":"2026-03-23T15:11:16Z","month":"03","status":"public","year":"2026","quality_controlled":"1","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","ddc":["580"],"external_id":{"pmid":["41831441"]},"file_date_updated":"2026-03-24T08:34:37Z","doi":"10.1016/j.cub.2026.02.023","oa_version":"Published Version","OA_place":"publisher","file":[{"access_level":"open_access","file_name":"2026_CurrentBiology_Li.pdf","date_created":"2026-03-24T08:34:37Z","date_updated":"2026-03-24T08:34:37Z","file_id":"21496","content_type":"application/pdf","creator":"dernst","checksum":"fe6c41fdab58a55df5f2a5860c02acdc","success":1,"file_size":12986894,"relation":"main_file"}],"pmid":1,"publication":"Current Biology","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","article_processing_charge":"Yes (via OA deal)"},{"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.71072"}],"date_published":"2026-03-11T00:00:00Z","corr_author":"1","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"publication_status":"epub_ahead","author":[{"last_name":"Babic","first_name":"David","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","full_name":"Babic, David"},{"full_name":"Zupunski, Milan","id":"f6a21fce-573e-11f0-a150-a8d96aee2539","first_name":"Milan","last_name":"Zupunski"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"date_created":"2026-03-23T14:59:06Z","status":"public","month":"03","year":"2026","title":"Imaging and genetic toolbox to study Arabidopsis embryogenesis","quality_controlled":"1","oa_version":"Published Version","external_id":{"pmid":["41808651"]},"doi":"10.1111/nph.71072","OA_place":"publisher","pmid":1,"publication":"New Phytologist","acknowledgement":"The authors would like to acknowledge the many colleagues whose valuable contributions to the field could not be included in this review due to space limitations and reference constraints. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ.","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","day":"11","publisher":"Wiley","_id":"21483","article_type":"original","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","article_number":"nph.71072","citation":{"ama":"Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 2026. doi:10.1111/nph.71072","short":"D. Babic, M. Zupunski, J. Friml, New Phytologist (2026).","mla":"Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist, nph. 71072, Wiley, 2026, doi:10.1111/nph.71072.","ista":"Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist., nph. 71072.","ieee":"D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study Arabidopsis embryogenesis,” New Phytologist. Wiley, 2026.","chicago":"Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist. Wiley, 2026. https://doi.org/10.1111/nph.71072.","apa":"Babic, D., Zupunski, M., & Friml, J. (2026). Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. Wiley. https://doi.org/10.1111/nph.71072"},"abstract":[{"text":"Embryogenesis in the model plant Arabidopsis thaliana provides a framework for understanding how cell polarity and patterning coordinate with hormonal signalling to establish the plant body plan. Following fertilisation, the zygote divides asymmetrically to generate apical and basal lineages, establishing the apical–basal axis that defines future shoot and root poles. Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical–basal axis establishment and organ initiation. The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors. Advances in microscopy have transformed the study of Arabidopsis embryogenesis: fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation, and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development, while laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity. Together, these methodological breakthroughs position Arabidopsis embryos as a prime model for dissecting the chemical and biophysical cues that shape plant development.","lang":"eng"}],"PlanS_conform":"1","language":[{"iso":"eng"}],"date_updated":"2026-03-30T05:58:35Z","type":"journal_article","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"_id":"20964","supervisor":[{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"related_material":{"record":[{"status":"public","id":"20982","relation":"part_of_dissertation"}]},"publisher":"Institute of Science and Technology Austria","day":"14","page":"22","type":"dissertation","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"}],"language":[{"iso":"eng"}],"date_updated":"2026-04-07T11:41:44Z","citation":{"ista":"Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria.","ieee":"D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026.","chicago":"Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-20964.","apa":"Vladimirtsev, D. (2026). Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20964","ama":"Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:10.15479/AT-ISTA-20964","short":"D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026.","mla":"Vladimirtsev, Dmitrii. Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-20964."},"has_accepted_license":"1","status":"public","month":"01","year":"2026","author":[{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii","last_name":"Vladimirtsev"}],"publication_identifier":{"issn":["2791-4585"]},"date_created":"2026-01-09T09:22:48Z","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"publication_status":"published","degree_awarded":"MS","date_published":"2026-01-14T00:00:00Z","corr_author":"1","article_processing_charge":"No","oa_version":"Published Version","doi":"10.15479/AT-ISTA-20964","file_date_updated":"2026-01-28T12:38:19Z","ddc":["570"],"file":[{"file_id":"21033","content_type":"application/pdf","embargo":"2027-01-01","creator":"dvladimi","checksum":"812857b2fbe3f6113bef22fd04bccd3e","file_size":2867531,"access_level":"closed","file_name":"2026_Vladimirtsev_Dmitrii_Thesis.pdf","date_updated":"2026-01-21T14:12:13Z","date_created":"2026-01-21T14:12:13Z","embargo_to":"open_access","relation":"main_file"},{"creator":"dvladimi","content_type":"application/x-zip-compressed","file_id":"21034","checksum":"2b969f97f8d7461bea3d255f48c2219c","file_size":25023066,"access_level":"closed","file_name":"Source Files.zip","date_updated":"2026-01-28T12:38:19Z","date_created":"2026-01-21T14:41:58Z","relation":"source_file"}],"OA_place":"publisher","title":"Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"alternative_title":["ISTA Master’s Thesis"]},{"OA_type":"gold","DOAJ_listed":"1","publisher":"Elsevier","day":"13","_id":"18619","article_type":"original","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"has_accepted_license":"1","issue":"1","article_number":"101181","citation":{"ieee":"H. Wei et al., “Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1,” Plant Communications, vol. 6, no. 1. Elsevier, 2025.","chicago":"Wei, H, H Zhu, W Ying, H Janssens, M Kvasnica, JM Winne, Y Gao, et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications. Elsevier, 2025. https://doi.org/10.1016/j.xplc.2024.101181.","apa":"Wei, H., Zhu, H., Ying, W., Janssens, H., Kvasnica, M., Winne, J., … Sun, L. (2025). Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101181","ista":"Wei H, Zhu H, Ying W, Janssens H, Kvasnica M, Winne J, Gao Y, Friml J, Ma Q, Tan S, Liu X, Russinova E, Sun L. 2025. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 6(1), 101181.","mla":"Wei, H., et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications, vol. 6, no. 1, 101181, Elsevier, 2025, doi:10.1016/j.xplc.2024.101181.","ama":"Wei H, Zhu H, Ying W, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 2025;6(1). doi:10.1016/j.xplc.2024.101181","short":"H. Wei, H. Zhu, W. Ying, H. Janssens, M. Kvasnica, J. Winne, Y. Gao, J. Friml, Q. Ma, S. Tan, X. Liu, E. Russinova, L. Sun, Plant Communications 6 (2025)."},"abstract":[{"text":"Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signalling initiation, which requires binding of BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter, ABCB19, functions as a BR exporter, and together with its close homologue, ABCB1, positively regulate BR signalling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 was stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states were determined. In the BL-bound structure, BL was bound to the hydrophobic cavity formed by the transmembrane domain, and triggered local conformational changes. Together, our data provide additional insights into the ABC transporter-mediated BR export.","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2025-05-19T14:02:01Z","volume":6,"type":"journal_article","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-01-13T00:00:00Z","department":[{"_id":"JiFr"}],"publication_status":"published","author":[{"last_name":"Wei","first_name":"H","full_name":"Wei, H"},{"last_name":"Zhu","first_name":"H","full_name":"Zhu, H"},{"full_name":"Ying, W","first_name":"W","last_name":"Ying"},{"first_name":"H","last_name":"Janssens","full_name":"Janssens, H"},{"full_name":"Kvasnica, M","last_name":"Kvasnica","first_name":"M"},{"first_name":"JM","last_name":"Winne","full_name":"Winne, JM"},{"full_name":"Gao, Y","last_name":"Gao","first_name":"Y"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"first_name":"Q","last_name":"Ma","full_name":"Ma, Q"},{"full_name":"Tan, S","first_name":"S","last_name":"Tan"},{"last_name":"Liu","first_name":"X","full_name":"Liu, X"},{"last_name":"Russinova","first_name":"E","full_name":"Russinova, E"},{"full_name":"Sun, L","first_name":"L","last_name":"Sun"}],"publication_identifier":{"issn":["2590-3462"]},"intvolume":" 6","isi":1,"date_created":"2024-12-04T11:21:16Z","status":"public","month":"01","year":"2025","title":"Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1","quality_controlled":"1","scopus_import":"1","doi":"10.1016/j.xplc.2024.101181","file_date_updated":"2025-04-16T09:02:05Z","oa_version":"Published Version","external_id":{"pmid":["39497419"],"isi":["001416757300001"]},"ddc":["580"],"OA_place":"publisher","file":[{"relation":"main_file","date_updated":"2025-04-16T09:02:05Z","date_created":"2025-04-16T09:02:05Z","file_name":"2025_PlantComm_Wei.pdf","access_level":"open_access","file_size":4443183,"checksum":"7b0e4511e43cc0da06730c3edb7c1167","success":1,"content_type":"application/pdf","file_id":"19575","creator":"dernst"}],"publication":"Plant Communications","pmid":1,"acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We thank Yaowei Wang, Yongming Luo, and Nemanja Vukašinović (VIB-UGhent, Belgium) for useful discussions and technical support. L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation. No conflict of interest is declared.","article_processing_charge":"Yes"},{"date_published":"2025-07-22T00:00:00Z","department":[{"_id":"JiFr"}],"publication_status":"published","author":[{"last_name":"Guan","first_name":"Bin","id":"56aad729-cca2-11ed-a45a-9b4138991a48","full_name":"Guan, Bin"},{"full_name":"Xie, Ke Xuan","first_name":"Ke Xuan","last_name":"Xie"},{"full_name":"Du, Xin Qiao","first_name":"Xin Qiao","last_name":"Du"},{"full_name":"Bai, Yu Xuan","last_name":"Bai","first_name":"Yu Xuan"},{"full_name":"Hao, Peng Chao","last_name":"Hao","first_name":"Peng Chao"},{"first_name":"Wen Hui","last_name":"Lin","full_name":"Lin, Wen Hui"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Xue, Hong Wei","last_name":"Xue","first_name":"Hong Wei"}],"intvolume":" 44","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"isi":1,"date_created":"2025-07-20T22:02:01Z","status":"public","month":"07","year":"2025","title":"Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD","quality_controlled":"1","scopus_import":"1","file_date_updated":"2025-07-22T08:52:17Z","oa_version":"Published Version","external_id":{"pmid":["40668679"],"isi":["001533244800001"]},"doi":"10.1016/j.celrep.2025.116024","ddc":["580"],"file":[{"checksum":"ee03deee47a084b0295251dc49470ad4","success":1,"file_size":37708120,"file_id":"20067","content_type":"application/pdf","creator":"dernst","date_created":"2025-07-22T08:52:17Z","date_updated":"2025-07-22T08:52:17Z","access_level":"open_access","file_name":"2025_CellReports_Guan.pdf","relation":"main_file"}],"OA_place":"publisher","publication":"Cell Reports","pmid":1,"acknowledgement":"The study was supported by National Natural Science Foundation of China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were run on the Siyuan-1 cluster supported by the Center for High-Performance Computing at Shanghai Jiao Tong University.","article_processing_charge":"Yes (in subscription journal)","OA_type":"hybrid","day":"22","publisher":"Elsevier","_id":"20029","article_type":"original","has_accepted_license":"1","tmp":{"image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"issue":"7","article_number":"116024","citation":{"ista":"Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 44(7), 116024.","chicago":"Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports. Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.116024.","ieee":"B. Guan et al., “Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD,” Cell Reports, vol. 44, no. 7. Elsevier, 2025.","apa":"Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue, H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2025.116024","ama":"Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 2025;44(7). doi:10.1016/j.celrep.2025.116024","short":"B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W. Xue, Cell Reports 44 (2025).","mla":"Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports, vol. 44, no. 7, 116024, Elsevier, 2025, doi:10.1016/j.celrep.2025.116024."},"abstract":[{"text":"Vacuolar acidification is crucial for the homeostasis of intracellular pH and the recycling of proteins and nutrients in cells, thereby playing important roles in various physiological processes related to vacuolar function. The key factors regulating vacuolar acidification and underlying mechanisms remain unclear. Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency. The pldζ2 mutant massively accumulates autophagic structures while exhibiting premature leaf senescence under nutrient starvation. Impaired autophagic flux, lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2 directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote vacuolar acidification and autophagy under phosphorus starvation. These findings reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide clues in elucidating the regulatory mechanism of vacuolar acidification.","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2025-09-30T14:05:28Z","volume":44,"type":"journal_article","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"page":"2700-2714","OA_type":"closed access","_id":"20031","article_type":"original","publisher":"Oxford University Press","day":"02","citation":{"mla":"Osorio-Navarro, Claudio, et al. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” Journal of Experimental Botany, vol. 76, no. 10, Oxford University Press, 2025, pp. 2700–14, doi:10.1093/jxb/eraf084.","ama":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 2025;76(10):2700-2714. doi:10.1093/jxb/eraf084","short":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, L. Norambuena, Journal of Experimental Botany 76 (2025) 2700–2714.","chicago":"Osorio-Navarro, Claudio, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, and Lorena Norambuena. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” Journal of Experimental Botany. Oxford University Press, 2025. https://doi.org/10.1093/jxb/eraf084.","ieee":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, and L. Norambuena, “The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana,” Journal of Experimental Botany, vol. 76, no. 10. Oxford University Press, pp. 2700–2714, 2025.","apa":"Osorio-Navarro, C., Neira-Valenzuela, G., Sierra, P., Adamowski, M., Toledo, J., & Norambuena, L. (2025). The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/eraf084","ista":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. 2025. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 76(10), 2700–2714."},"issue":"10","type":"journal_article","volume":76,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"abstract":[{"text":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which has not been well described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference with clathrin-mediated endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-trans-Golgi network (TGN)/early endosome (EE) trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during vacuole configuration, most probably carrying the material that allows the establishment of the vacuole in elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional players must be defined.","lang":"eng"}],"date_updated":"2025-09-30T14:04:16Z","department":[{"_id":"JiFr"}],"publication_status":"published","date_published":"2025-07-02T00:00:00Z","month":"07","status":"public","year":"2025","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"intvolume":" 76","author":[{"last_name":"Osorio-Navarro","first_name":"Claudio","full_name":"Osorio-Navarro, Claudio"},{"full_name":"Neira-Valenzuela, Gabriel","first_name":"Gabriel","last_name":"Neira-Valenzuela"},{"full_name":"Sierra, Paula","last_name":"Sierra","first_name":"Paula"},{"orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek","first_name":"Maciek","last_name":"Adamowski"},{"last_name":"Toledo","first_name":"Jorge","full_name":"Toledo, Jorge"},{"full_name":"Norambuena, Lorena","last_name":"Norambuena","first_name":"Lorena"}],"date_created":"2025-07-20T22:02:01Z","isi":1,"oa_version":"None","doi":"10.1093/jxb/eraf084","external_id":{"pmid":["40056424"],"isi":["001482869200001"]},"quality_controlled":"1","title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana","scopus_import":"1","article_processing_charge":"No","publication":"Journal of Experimental Botany","pmid":1,"acknowledgement":"This research was supported by FONDECYT grants 1170950 and 1211311 and by ANID PhD fellowship 2020-21201663 to PhD student CO-N. The microscopes used in this work were funded by grants FONDEQUIP #EQM 140019 and #EQM12-0003 at the Advanced Microscopy Unit of the Biology Department, Faculty of Science, University of Chile.\r\nWe thank Jiri Friml for donating the XVE»AUXILIN-LIKE2 (AX2) line to support our research. We wish to acknowledge the active and helpful discussion of all the members of the LNM team and the Plant Molecular Biology Centre at Universidad de Chile."},{"month":"07","status":"public","year":"2025","publication_identifier":{"eissn":["2211-1247"]},"intvolume":" 44","author":[{"full_name":"Xu, Faqing","last_name":"Xu","first_name":"Faqing"},{"full_name":"Yu, Yongqiang","last_name":"Yu","first_name":"Yongqiang"},{"last_name":"Guan","first_name":"Bin","full_name":"Guan, Bin","id":"56aad729-cca2-11ed-a45a-9b4138991a48"},{"full_name":"Xu, Tongda","first_name":"Tongda","last_name":"Xu"},{"last_name":"Xu","first_name":"Zhihong","full_name":"Xu, Zhihong"},{"full_name":"Xue, Hongwei","last_name":"Xue","first_name":"Hongwei"}],"date_created":"2025-08-04T13:39:11Z","isi":1,"department":[{"_id":"JiFr"}],"publication_status":"published","date_published":"2025-07-24T00:00:00Z","article_processing_charge":"Yes","pmid":1,"publication":"Cell Reports","acknowledgement":"The study was supported by the National Natural Science Foundation of China (NSFC; 32230011, 91954206, and 31721001). We thank Dr. Deli Lin (Shanghai Jiao Tong University) for kind help with the laser confocal microscope observation and the Arabidopsis Biological Resource Center (ABRC) for providing T-DNA insertional mutants.","ddc":["580"],"file_date_updated":"2025-08-05T06:15:09Z","oa_version":"Published Version","external_id":{"pmid":["40714631"],"isi":["001542038500001"]},"doi":"10.1016/j.celrep.2025.116056","OA_place":"publisher","file":[{"relation":"main_file","content_type":"application/pdf","creator":"dernst","file_id":"20120","file_size":24178018,"checksum":"3c43e040a4a7a65ec67ae1d2bb81261a","success":1,"file_name":"2025_CellReports_Xu.pdf","access_level":"open_access","date_updated":"2025-08-05T06:15:09Z","date_created":"2025-08-05T06:15:09Z"}],"quality_controlled":"1","title":"Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis","scopus_import":"1","article_type":"original","_id":"20116","publisher":"Elsevier","day":"24","DOAJ_listed":"1","OA_type":"gold","type":"journal_article","volume":44,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa":1,"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Auxin regulates various aspects of plant growth and development by modulating the transcription of target genes through the degradation of auxin/indole-3-acetic acid (Aux/IAA) repressors via the 26S proteasome. Proteasome regulator 1 (PTRE1), a positive regulator of proteasome activity, has been implicated in auxin-mediated proteasome suppression; however, the mechanism by which auxin modulates PTRE1 function remains unclear. Here, we demonstrate that auxin promotes the interaction between germin-like protein 1 (GLP1) and PTRE1, facilitating PTRE1 retention at the plasma membrane. The relocation of PTRE1 results in reduced nuclear 26S proteasome activity, and thus the attenuated Aux/IAA degradation and altered Aux/IAA homeostasis, ultimately resulting in suppressed auxin-mediated transcriptional regulation. Our findings uncover a previously uncharacterized regulatory axis in auxin signaling that controls Aux/IAA protein stability, functioning alongside the TIR1- and TRANSMEMBRANE KINASE 1 (TMK1)-mediated pathways, and highlight the coordination of auxin signaling from the cell surface to the nucleus via auxin-induced PTRE1 relocation, which fine-tunes Aux/IAA protein homeostasis and auxin responses."}],"date_updated":"2025-09-30T14:13:45Z","citation":{"apa":"Xu, F., Yu, Y., Guan, B., Xu, T., Xu, Z., & Xue, H. (2025). Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2025.116056","ieee":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, and H. Xue, “Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis,” Cell Reports, vol. 44, no. 8. Elsevier, 2025.","chicago":"Xu, Faqing, Yongqiang Yu, Bin Guan, Tongda Xu, Zhihong Xu, and Hongwei Xue. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” Cell Reports. Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.116056.","ista":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. 2025. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. 44(8), 116056.","mla":"Xu, Faqing, et al. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” Cell Reports, vol. 44, no. 8, 116056, Elsevier, 2025, doi:10.1016/j.celrep.2025.116056.","short":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, H. Xue, Cell Reports 44 (2025).","ama":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. 2025;44(8). doi:10.1016/j.celrep.2025.116056"},"has_accepted_license":"1","tmp":{"image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"article_number":"116056","issue":"8"},{"publisher":"National Academy of Sciences","day":"23","article_type":"original","_id":"20635","OA_type":"hybrid","page":"e2512274122","date_updated":"2026-02-16T12:32:51Z","language":[{"iso":"eng"}],"PlanS_conform":"1","abstract":[{"lang":"eng","text":"Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s-mediated phosphorylation modulates both the polar localization and transport activity of PIN2. Importantly, SnRK2s function antagonistically to the AGCVIII kinase PID, which phosphorylates PIN2 at a distinct site (S258), establishing a regulatory balance essential for adaptive root growth. Structural modeling and phosphorylation assays further suggest that SnRK2s-mediated phosphorylation at S259 sterically hinders access of PID to S258, providing a mechanistic basis for their antagonistic relationship. These findings uncover a novel regulatory mechanism, by which plants fine-tune root developmental programs to adapt to environmental stimuli, highlighting the evolutionary significance of multilayered kinase-mediated regulation in plant adaptation."}],"project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"type":"journal_article","volume":122,"issue":"39","has_accepted_license":"1","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"citation":{"ista":"Sheng F, Gao Y, Wang Y, Li Y, Zhang J, Zhang Z, Qin X, Zhang S, Song W, Li J, Guo Y, Friml J, Gong Z, Zhang Q, Zhang J. 2025. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 122(39), e2512274122.","apa":"Sheng, F., Gao, Y., Wang, Y., Li, Y., Zhang, J., Zhang, Z., … Zhang, J. (2025). Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2512274122","ieee":"F. Sheng et al., “Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism,” Proceedings of the National Academy of Sciences, vol. 122, no. 39. National Academy of Sciences, p. e2512274122, 2025.","chicago":"Sheng, F, Y Gao, Y Wang, Y Li, JA Zhang, Z Zhang, X Qin, et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2512274122.","short":"F. Sheng, Y. Gao, Y. Wang, Y. Li, J. Zhang, Z. Zhang, X. Qin, S. Zhang, W. Song, J. Li, Y. Guo, J. Friml, Z. Gong, Q. Zhang, J. Zhang, Proceedings of the National Academy of Sciences 122 (2025) e2512274122.","ama":"Sheng F, Gao Y, Wang Y, et al. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 2025;122(39):e2512274122. doi:10.1073/pnas.2512274122","mla":"Sheng, F., et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences, vol. 122, no. 39, National Academy of Sciences, 2025, p. e2512274122, doi:10.1073/pnas.2512274122."},"date_created":"2025-11-12T10:03:20Z","isi":1,"intvolume":" 122","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"author":[{"full_name":"Sheng, F","first_name":"F","last_name":"Sheng"},{"full_name":"Gao, Y","last_name":"Gao","first_name":"Y"},{"full_name":"Wang, Y","last_name":"Wang","first_name":"Y"},{"last_name":"Li","first_name":"Y","full_name":"Li, Y"},{"first_name":"JA","last_name":"Zhang","full_name":"Zhang, JA"},{"last_name":"Zhang","first_name":"Z","full_name":"Zhang, Z"},{"first_name":"X","last_name":"Qin","full_name":"Qin, X"},{"last_name":"Zhang","first_name":"S","full_name":"Zhang, S"},{"full_name":"Song, W","first_name":"W","last_name":"Song"},{"last_name":"Li","first_name":"J","full_name":"Li, J"},{"first_name":"Y","last_name":"Guo","full_name":"Guo, Y"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"full_name":"Gong, Z","first_name":"Z","last_name":"Gong"},{"full_name":"Zhang, Q","last_name":"Zhang","first_name":"Q"},{"last_name":"Zhang","first_name":"J","full_name":"Zhang, J"}],"year":"2025","month":"09","status":"public","date_published":"2025-09-23T00:00:00Z","publication_status":"published","department":[{"_id":"JiFr"}],"acknowledgement":"This research was funded by Biological Breeding-National Science and Technology Major Project (2023ZD0407201), China Postdoctoral Science Foundation (2024M763575), China Agricultural University Fund (2025RC042), Chinese Universities Scientific Fund (2024RC031), and Austrian Science Fund (FWF; I 6123-B).","publication":"Proceedings of the National Academy of Sciences","pmid":1,"article_processing_charge":"Yes (in subscription journal)","scopus_import":"1","quality_controlled":"1","title":"Antagonistic SnRK2 and PID kinases' action on auxin transport-mediated root gravitropism","file":[{"file_id":"20681","content_type":"application/pdf","creator":"dernst","file_size":2667764,"checksum":"38b723a909bf321d7ee537c9d064aa25","success":1,"file_name":"2025_PNAS_Sheng.pdf","access_level":"open_access","date_updated":"2025-11-24T13:48:09Z","date_created":"2025-11-24T13:48:09Z","relation":"main_file"}],"OA_place":"publisher","ddc":["580"],"file_date_updated":"2025-11-24T13:48:09Z","oa_version":"Published Version","doi":"10.1073/pnas.2512274122","external_id":{"isi":["001589177800001"],"pmid":["40986351"]}},{"article_processing_charge":"No","acknowledgement":"We thank Lukáš Fiedler for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","pmid":1,"publication":"Developmental Cell","external_id":{"pmid":["41043435"]},"doi":"10.1016/j.devcel.2025.09.009","oa_version":"None","scopus_import":"1","title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","quality_controlled":"1","year":"2025","status":"public","month":"10","date_created":"2025-11-12T10:03:39Z","author":[{"full_name":"Huang, R","last_name":"Huang","first_name":"R"},{"full_name":"Wang, J","first_name":"J","last_name":"Wang"},{"first_name":"M","last_name":"Chang","full_name":"Chang, M"},{"full_name":"Tang, W","last_name":"Tang","first_name":"W"},{"last_name":"Yu","first_name":"Y","full_name":"Yu, Y"},{"last_name":"Zhang","first_name":"Y","full_name":"Zhang, Y"},{"first_name":"Y","last_name":"Peng","full_name":"Peng, Y"},{"full_name":"Wang, Y","last_name":"Wang","first_name":"Y"},{"full_name":"Guo, Y","first_name":"Y","last_name":"Guo"},{"first_name":"T","last_name":"Lu","full_name":"Lu, T"},{"first_name":"Y","last_name":"Cao","full_name":"Cao, Y"},{"full_name":"Zhou, Y","last_name":"Zhou","first_name":"Y"},{"full_name":"Zhang, Q","first_name":"Q","last_name":"Zhang"},{"full_name":"Huang, Y","first_name":"Y","last_name":"Huang"},{"first_name":"A","last_name":"Wu","full_name":"Wu, A"},{"first_name":"L","last_name":"Ren","full_name":"Ren, L"},{"last_name":"Gallei","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368"},{"full_name":"Dong, J","first_name":"J","last_name":"Dong"},{"first_name":"H","last_name":"Chen","full_name":"Chen, H"},{"first_name":"J","last_name":"He","full_name":"He, J"},{"last_name":"Wen","first_name":"M","full_name":"Wen, M"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Sun","first_name":"L","full_name":"Sun, L"},{"full_name":"Xiong, Y","first_name":"Y","last_name":"Xiong"},{"full_name":"Yang, Z","last_name":"Yang","first_name":"Z"},{"full_name":"Xu, T","last_name":"Xu","first_name":"T"}],"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"publication_status":"epub_ahead","department":[{"_id":"JiFr"}],"date_published":"2025-10-02T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","date_updated":"2025-11-24T13:43:08Z","abstract":[{"text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis.","lang":"eng"}],"language":[{"iso":"eng"}],"citation":{"ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 2025:S1534-5807(25)00569-6. doi:10.1016/j.devcel.2025.09.009","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell (2025) S1534-5807(25)00569–6.","mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell, Elsevier, 2025, pp. S1534-5807(25)00569-6, doi:10.1016/j.devcel.2025.09.009.","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2025. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell., S1534-5807(25)00569–6.","ieee":"R. Huang et al., “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” Developmental Cell. Elsevier, pp. S1534-5807(25)00569–6, 2025.","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.09.009.","apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2025). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.09.009"},"_id":"20636","article_type":"original","publisher":"Elsevier","day":"02","page":"S1534-5807(25)00569-6","OA_type":"closed access"},{"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"grant_number":"ALTF 985-2016","_id":"26060676-B435-11E9-9278-68D0E5697425","name":"Cell surface receptor complexes for auxin signaling in plants"},{"name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","call_identifier":"FP7"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"volume":188,"type":"journal_article","date_updated":"2025-12-01T15:27:22Z","language":[{"iso":"eng"}],"PlanS_conform":"1","abstract":[{"lang":"eng","text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena."}],"citation":{"short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml, Cell 188 (2025) 6138–6150.e17.","ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 2025;188(22):6138-6150.e17. doi:10.1016/j.cell.2025.08.026","mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell, vol. 188, no. 22, Elsevier, 2025, p. 6138–6150.e17, doi:10.1016/j.cell.2025.08.026.","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J. 2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. Elsevier. https://doi.org/10.1016/j.cell.2025.08.026","ieee":"L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism,” Cell, vol. 188, no. 22. Elsevier, p. 6138–6150.e17, 2025.","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell. Elsevier, 2025. https://doi.org/10.1016/j.cell.2025.08.026."},"issue":"22","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"_id":"20656","article_type":"original","day":"30","publisher":"Elsevier","related_material":{"record":[{"relation":"earlier_version","id":"19399","status":"public"}]},"page":"6138-6150.e17","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","acknowledgement":"We gratefully acknowledge Tongda Xu for experimental, material, and conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at the Institute of Science and Technology Austria (ISTA) for their excellent service and assistance. The research leading to these results has received funding from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003 implemented within the Palacký University Young Researcher Grant.","pmid":1,"publication":"Cell","OA_place":"publisher","file":[{"relation":"main_file","access_level":"open_access","file_name":"2025_Cell_Rodriguez.pdf","date_updated":"2025-11-24T10:55:18Z","date_created":"2025-11-24T10:55:18Z","creator":"dernst","content_type":"application/pdf","file_id":"20679","success":1,"checksum":"8ac396a0806ad7f2e4e7a0c1eed712ce","file_size":17825465}],"ddc":["580"],"doi":"10.1016/j.cell.2025.08.026","external_id":{"pmid":["41043433"],"isi":["001616077900005"]},"oa_version":"Published Version","file_date_updated":"2025-11-24T10:55:18Z","quality_controlled":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism","year":"2025","month":"10","status":"public","date_created":"2025-11-19T09:44:31Z","isi":1,"intvolume":" 188","publication_identifier":{"issn":["0092-8674"]},"author":[{"full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7244-7237","last_name":"Rodriguez Solovey","first_name":"Lesia"},{"full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","first_name":"Lukas","last_name":"Fiedler"},{"first_name":"Minxia","last_name":"Zou","full_name":"Zou, Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9"},{"last_name":"Giannini","first_name":"Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina"},{"first_name":"Aline","last_name":"Monzer","full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"last_name":"Vladimirtsev","first_name":"Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii"},{"last_name":"Randuch","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek"},{"first_name":"Yongfan","last_name":"Yu","full_name":"Yu, Yongfan"},{"last_name":"Gelová","first_name":"Zuzana","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana","orcid":"0000-0003-4783-1752"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten"},{"first_name":"Jakub","last_name":"Hajny","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub"},{"full_name":"Chen, Meng","first_name":"Meng","last_name":"Chen"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","last_name":"Tan","first_name":"Shutang"},{"full_name":"Hörmayer, Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926","last_name":"Hörmayer","first_name":"Lukas"},{"first_name":"Lanxin","last_name":"Li","orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin"},{"first_name":"Maria Mar","last_name":"Marques-Bueno","full_name":"Marques-Bueno, Maria Mar"},{"first_name":"Zainab","last_name":"Quddoos","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","full_name":"Quddoos, Zainab"},{"full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely","last_name":"Molnar"},{"first_name":"Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","full_name":"Kulich, Ivan"},{"last_name":"Jaillais","first_name":"Yvon","full_name":"Jaillais, Yvon"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"publication_status":"published","department":[{"_id":"JiFr"},{"_id":"XiFe"}],"corr_author":"1","date_published":"2025-10-30T00:00:00Z","ec_funded":1},{"department":[{"_id":"JiFr"}],"publication_status":"published","date_published":"2025-11-18T00:00:00Z","status":"public","month":"11","year":"2025","author":[{"full_name":"Roychoudhry, Suruchi","last_name":"Roychoudhry","first_name":"Suruchi"},{"full_name":"Sageman-Furnas, Katelyn","last_name":"Sageman-Furnas","first_name":"Katelyn"},{"full_name":"Taylor, Harry J.","last_name":"Taylor","first_name":"Harry J."},{"last_name":"Showpnil","first_name":"Iftekhar","full_name":"Showpnil, Iftekhar"},{"full_name":"Wolverton, Chris","first_name":"Chris","last_name":"Wolverton"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Bianco, Marta Del","last_name":"Bianco","first_name":"Marta Del"},{"first_name":"Stefan","last_name":"Kepinski","full_name":"Kepinski, Stefan"}],"publication_identifier":{"eissn":["1091-6490"]},"intvolume":" 122","date_created":"2025-11-23T23:01:38Z","file_date_updated":"2025-11-24T09:48:44Z","doi":"10.1073/pnas.2506400122","external_id":{"pmid":["41218119"]},"oa_version":"Published Version","ddc":["580"],"OA_place":"publisher","file":[{"file_name":"2025_PNAS_Roychoudhry.pdf","access_level":"open_access","date_created":"2025-11-24T09:48:44Z","date_updated":"2025-11-24T09:48:44Z","file_id":"20676","content_type":"application/pdf","creator":"dernst","file_size":1394055,"checksum":"5e1c37dddc5db8fbd0128db4a54c4f6b","success":1,"relation":"main_file"}],"title":"Angle dependence as a unifying feature of root graviresponse modules","quality_controlled":"1","scopus_import":"1","article_processing_charge":"Yes (in subscription journal)","pmid":1,"publication":"Proceedings of the National Academy of Sciences","acknowledgement":"This study was funded by the BBSRC (grant no. BB/N010124/1) and Leverhulme Foundation (grant no. RPG-2018-137) to M.D.B. and S.K., a Fully Funded International Research Scholarship awarded to K.S.-F., and by NASA (grant no. 80NSSC21K0585) to C.W.","page":"e2506400122","OA_type":"hybrid","article_type":"original","_id":"20663","publisher":"National Academy of Sciences","day":"18","citation":{"ista":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, Showpnil I, Wolverton C, Friml J, Bianco MD, Kepinski S. 2025. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 122(46), e2506400122.","ieee":"S. Roychoudhry et al., “Angle dependence as a unifying feature of root graviresponse modules,” Proceedings of the National Academy of Sciences, vol. 122, no. 46. National Academy of Sciences, p. e2506400122, 2025.","chicago":"Roychoudhry, Suruchi, Katelyn Sageman-Furnas, Harry J. Taylor, Iftekhar Showpnil, Chris Wolverton, Jiří Friml, Marta Del Bianco, and Stefan Kepinski. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2506400122.","apa":"Roychoudhry, S., Sageman-Furnas, K., Taylor, H. J., Showpnil, I., Wolverton, C., Friml, J., … Kepinski, S. (2025). Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2506400122","ama":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, et al. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 2025;122(46):e2506400122. doi:10.1073/pnas.2506400122","short":"S. Roychoudhry, K. Sageman-Furnas, H.J. Taylor, I. Showpnil, C. Wolverton, J. Friml, M.D. Bianco, S. Kepinski, Proceedings of the National Academy of Sciences 122 (2025) e2506400122.","mla":"Roychoudhry, Suruchi, et al. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences, vol. 122, no. 46, National Academy of Sciences, 2025, p. e2506400122, doi:10.1073/pnas.2506400122."},"has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"issue":"46","volume":122,"type":"journal_article","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","abstract":[{"lang":"eng","text":"Gravitropism, the patterning of postembryonic growth in relation to the gravity vector, allows plants to optimize the use of limited and nonhomogenous resources in their immediate environment. Since the current model of root gravitropism has not been able to integrate all aspects of the response (perception, response, and behavior), research on gravitropism has been dominated by different theories attempting to conceptualize each aspect individually. In this work, we sought to reevaluate all the main components of the root graviresponse through the lens of angle dependence. We show angle dependence in Cholodny–Went-based auxin asymmetry and growth response, which we tracked back to angle-dependent variation in PIN asymmetry and statolith sedimentation in the columella. Thanks to this approach, we were able to suggest distinct roles for PINs and columella cell tiers, and a potential function for auxin vertical flux through the columella. Our findings provide a unifying framework to further explore the mechanisms that regulate angle-dependent gravitropic response, with major implications of time-dependent features of root graviresponse."}],"language":[{"iso":"eng"}],"date_updated":"2026-02-16T12:31:31Z"},{"oa_version":"Published Version","external_id":{"pmid":["41249070"]},"doi":"10.1016/j.tplants.2025.10.018","ddc":["580"],"OA_place":"publisher","title":"Role of cAMP in TIR1/AFB auxin signaling: Open issues","scopus_import":"1","article_processing_charge":"Yes (via OA deal)","pmid":1,"publication":"Trends in Plant Science","acknowledgement":"I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input.","department":[{"_id":"JiFr"}],"publication_status":"epub_ahead","date_published":"2025-11-16T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1016/j.tplants.2025.10.018","open_access":"1"}],"corr_author":"1","status":"public","month":"11","year":"2025","author":[{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"publication_identifier":{"eissn":["1878-4372"],"issn":["1360-1385"]},"date_created":"2025-12-02T16:29:22Z","citation":{"apa":"Friml, J. (2025). Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2025.10.018","chicago":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science. Elsevier, 2025. https://doi.org/10.1016/j.tplants.2025.10.018.","ieee":"J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” Trends in Plant Science. Elsevier, pp. S1360-1385(25)00300–0, 2025.","ista":"Friml J. 2025. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science., S1360-1385(25)00300–0.","mla":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science, Elsevier, 2025, pp. S1360-1385(25)00300-0, doi:10.1016/j.tplants.2025.10.018.","short":"J. Friml, Trends in Plant Science (2025) S1360-1385(25)00300–0.","ama":"Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 2025:S1360-1385(25)00300-0. doi:10.1016/j.tplants.2025.10.018"},"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","type":"journal_article","oa":1,"project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","abstract":[{"text":"The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article.","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2025-12-09T08:04:58Z","page":"S1360-1385(25)00300-0","OA_type":"hybrid","_id":"20725","article_type":"review","publisher":"Elsevier","day":"16"},{"article_processing_charge":"No","acknowledgement":"The authors sincerely thank Dr. Shutang Tan for experimental support and Dr. Barbara Kloeckener Gruissem for critical reading and constructive advice on the manuscript. This study was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.), by the Ministry of Science and Technology (grant 112-2636-B-005-001- to K.-J.L.), and by the Ministry of Education (grant MOE-109-YSFAG-0006-001-P1 to K.-J.L.).","pmid":1,"publication":"Plant Cell and Environment","oa_version":"None","doi":"10.1111/pce.70295","external_id":{"pmid":["41340422"]},"scopus_import":"1","quality_controlled":"1","title":"The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha","year":"2025","month":"12","status":"public","date_created":"2025-12-14T23:02:05Z","publication_identifier":{"issn":["0140-7791"],"eissn":["1365-3040"]},"author":[{"orcid":"0000-0001-6152-6637","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","full_name":"Tang, Han","first_name":"Han","last_name":"Tang"},{"first_name":"Adrijana","last_name":"Smoljan","full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b"},{"first_name":"Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia"},{"full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","last_name":"Zhang","first_name":"Yuzhou"},{"full_name":"Lu, Kuan Ju","first_name":"Kuan Ju","last_name":"Lu"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"publication_status":"epub_ahead","department":[{"_id":"JiFr"}],"date_published":"2025-12-03T00:00:00Z","ec_funded":1,"project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","date_updated":"2025-12-15T13:56:26Z","language":[{"iso":"eng"}],"abstract":[{"text":"This study demonstrates that Marchantia non-canonical PINs are predominantly localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric distribution.\r\nA newly identified miniW domain within the MpPINW hydrophilic loop governs subcellular trafficking and asymmetric PM localization of non-canonical PINs in Marchantia.","lang":"eng"}],"citation":{"ista":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. 2025. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment.","ieee":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K. J. Lu, and J. Friml, “The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha,” Plant Cell and Environment. Wiley, 2025.","chicago":"Tang, Han, Adrijana Smoljan, Minxia Zou, Yuzhou Zhang, Kuan Ju Lu, and Jiří Friml. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment. Wiley, 2025. https://doi.org/10.1111/pce.70295.","apa":"Tang, H., Smoljan, A., Zou, M., Zhang, Y., Lu, K. J., & Friml, J. (2025). The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. Wiley. https://doi.org/10.1111/pce.70295","ama":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. 2025. doi:10.1111/pce.70295","short":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K.J. Lu, J. Friml, Plant Cell and Environment (2025).","mla":"Tang, Han, et al. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment, Wiley, 2025, doi:10.1111/pce.70295."},"article_type":"comment","_id":"20818","publisher":"Wiley","day":"03","OA_type":"closed access"},{"ec_funded":1,"date_published":"2025-04-01T00:00:00Z","corr_author":"1","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"publication_status":"published","author":[{"orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","first_name":"Michelle C","last_name":"Gallei"},{"id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M","last_name":"Truckenbrodt","first_name":"Sven M"},{"full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger"},{"full_name":"Inumella, Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","orcid":"0009-0002-5890-120X","last_name":"Inumella","first_name":"Syamala"},{"last_name":"Vistunou","first_name":"Vitali","full_name":"Vistunou, Vitali","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81"},{"first_name":"Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba","orcid":"0000-0002-7667-6854","last_name":"Tavakoli","first_name":"Mojtaba"},{"last_name":"Agudelo Duenas","first_name":"Nathalie","full_name":"Agudelo Duenas, Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jakob","last_name":"Vorlaufer","orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425"},{"full_name":"Jahr, Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","first_name":"Wiebke","last_name":"Jahr"},{"first_name":"Marek","last_name":"Randuch","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Alexander J","last_name":"Johnson","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Danzl","first_name":"Johann G","full_name":"Danzl, Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973"}],"publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"intvolume":" 37","date_created":"2025-02-05T06:52:06Z","isi":1,"status":"public","month":"04","year":"2025","title":"Super-resolution expansion microscopy in plant roots","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"quality_controlled":"1","scopus_import":"1","file_date_updated":"2025-07-31T07:03:43Z","oa_version":"Published Version","doi":"10.1093/plcell/koaf006","external_id":{"pmid":["39792900"],"isi":["001462763100001"]},"ddc":["580"],"file":[{"content_type":"application/pdf","file_id":"20092","creator":"dernst","file_size":53904111,"success":1,"checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","file_name":"2025_PlantCell_Gallei.pdf","access_level":"open_access","date_created":"2025-07-31T07:03:43Z","date_updated":"2025-07-31T07:03:43Z","relation":"main_file"}],"OA_place":"publisher","pmid":1,"publication":"The Plant Cell","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","related_material":{"record":[{"relation":"earlier_version","status":"public","id":"18689"},{"id":"18837","status":"public","relation":"research_data"}]},"day":"01","publisher":"Oxford University Press","article_type":"original","_id":"19003","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"issue":"4","article_number":"koaf006","citation":{"ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University Press, 2025. https://doi.org/10.1093/plcell/koaf006.","ieee":"M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:10.1093/plcell/koaf006."},"PlanS_conform":"1","abstract":[{"text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2025-10-08T08:43:56Z","type":"journal_article","volume":37,"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"call_identifier":"FWF","grant_number":"W1232-B24","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets"}]},{"article_processing_charge":"No","acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","publication":"Plant and Cell Physiology","pmid":1,"oa_version":"None","doi":"10.1093/pcp/pcaf008","external_id":{"pmid":["39829340"],"isi":["001436802900001"]},"scopus_import":"1","title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","quality_controlled":"1","year":"2025","status":"public","month":"03","date_created":"2025-03-19T09:44:19Z","isi":1,"author":[{"full_name":"Tang, Han","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","orcid":"0000-0001-6152-6637","last_name":"Tang","first_name":"Han"},{"full_name":"Chen, L","last_name":"Chen","first_name":"L"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"publication_status":"published","department":[{"_id":"JiFr"}],"corr_author":"1","ec_funded":1,"date_published":"2025-03-05T00:00:00Z","project":[{"call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","date_updated":"2025-09-30T11:05:55Z","abstract":[{"lang":"eng","text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species."}],"language":[{"iso":"eng"}],"citation":{"apa":"Tang, H., Chen, L., & Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcaf008","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” Plant and Cell Physiology. Oxford University Press, 2025.","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology. Oxford University Press, 2025. https://doi.org/10.1093/pcp/pcaf008.","ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology, pcaf008, Oxford University Press, 2025, doi:10.1093/pcp/pcaf008.","short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. 2025. doi:10.1093/pcp/pcaf008"},"article_number":"pcaf008","_id":"19420","article_type":"original","day":"05","publisher":"Oxford University Press","OA_type":"closed access"},{"date_updated":"2025-11-12T07:52:06Z","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"type":"journal_article","volume":11,"article_number":"5207","has_accepted_license":"1","citation":{"apa":"Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025). Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01934-w","ieee":"G. Jia et al., “Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize,” Nature Plants, vol. 11. Springer Nature, 2025.","chicago":"Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01934-w.","ista":"Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.","mla":"Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants, vol. 11, 5207, Springer Nature, 2025, doi:10.1038/s41477-025-01934-w.","short":"G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X. Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants 11 (2025).","ama":"Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 2025;11. doi:10.1038/s41477-025-01934-w"},"publisher":"Springer Nature","day":"05","_id":"19422","article_type":"original","OA_type":"green","acknowledgement":"We thank X. Yang for providing published inbred lines and helping with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful discussions. The transgenic maize lines were generated by the Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265 and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.), the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.), the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.), and the Central Guidance on Local Science and Technology Development Fund of Shanxi Province (YDZJSX2024D040 to C.T. and J.Z.).","pmid":1,"publication":"Nature Plants","article_processing_charge":"No","scopus_import":"1","quality_controlled":"1","title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","OA_place":"repository","file":[{"relation":"main_file","access_level":"open_access","file_name":"2025_NaturePlants_Jia_submitted.pdf","date_updated":"2025-11-12T07:50:45Z","date_created":"2025-11-12T07:50:45Z","creator":"dernst","file_id":"20634","content_type":"application/pdf","checksum":"caeaf1a8bc3e1435e8c995d1d9df5390","success":1,"file_size":2714177}],"ddc":["580"],"oa_version":"Submitted Version","file_date_updated":"2025-11-12T07:50:45Z","doi":"10.1038/s41477-025-01934-w","external_id":{"isi":["001437953800001"],"pmid":["40044942"]},"isi":1,"date_created":"2025-03-19T09:44:55Z","publication_identifier":{"issn":["2055-0278"]},"intvolume":" 11","author":[{"first_name":"G","last_name":"Jia","full_name":"Jia, G"},{"full_name":"Chen, G","last_name":"Chen","first_name":"G"},{"last_name":"Zhang","first_name":"Z","full_name":"Zhang, Z"},{"full_name":"Tian, C","last_name":"Tian","first_name":"C"},{"last_name":"Wang","first_name":"Y","full_name":"Wang, Y"},{"last_name":"Luo","first_name":"J","full_name":"Luo, J"},{"full_name":"Zhang, K","first_name":"K","last_name":"Zhang"},{"last_name":"Zhao","first_name":"X","full_name":"Zhao, X"},{"last_name":"Zhao","first_name":"X","full_name":"Zhao, X"},{"full_name":"Li, Z","last_name":"Li","first_name":"Z"},{"first_name":"L","last_name":"Sun","full_name":"Sun, L"},{"full_name":"Yang, W","last_name":"Yang","first_name":"W"},{"first_name":"Y","last_name":"Guo","full_name":"Guo, Y"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"full_name":"Gong, Z","last_name":"Gong","first_name":"Z"},{"last_name":"Zhang","first_name":"J","full_name":"Zhang, J"}],"year":"2025","month":"03","status":"public","date_published":"2025-03-05T00:00:00Z","publication_status":"published","department":[{"_id":"JiFr"}]},{"OA_type":"hybrid","page":"1066-1083","publisher":"Wiley","day":"01","article_type":"original","_id":"19423","issue":"3","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","citation":{"short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083.","ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 2025;246(3):1066-1083. doi:10.1111/nph.70019","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:10.1111/nph.70019.","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. Wiley. https://doi.org/10.1111/nph.70019","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist. Wiley, 2025. https://doi.org/10.1111/nph.70019.","ieee":"K. Kurtović et al., “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” New Phytologist, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025."},"date_updated":"2025-09-30T11:11:18Z","language":[{"iso":"eng"}],"abstract":[{"text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.","lang":"eng"}],"project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa":1,"type":"journal_article","volume":246,"date_published":"2025-05-01T00:00:00Z","publication_status":"published","department":[{"_id":"JiFr"}],"date_created":"2025-03-19T09:45:11Z","isi":1,"intvolume":" 246","publication_identifier":{"issn":["1469-8137"]},"author":[{"full_name":"Kurtović, K","first_name":"K","last_name":"Kurtović"},{"first_name":"S","last_name":"Vosolsobě","full_name":"Vosolsobě, S"},{"first_name":"D","last_name":"Nedvěd","full_name":"Nedvěd, D"},{"full_name":"Müller, K","first_name":"K","last_name":"Müller"},{"first_name":"PI","last_name":"Dobrev","full_name":"Dobrev, PI"},{"full_name":"Schmidt, V","last_name":"Schmidt","first_name":"V"},{"last_name":"Piszczek","first_name":"P","full_name":"Piszczek, P"},{"full_name":"Kuhn, A","last_name":"Kuhn","first_name":"A"},{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana","last_name":"Smoljan"},{"first_name":"TJ","last_name":"Fisher","full_name":"Fisher, TJ"},{"first_name":"D","last_name":"Weijers","full_name":"Weijers, D"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"JL","last_name":"Bowman","full_name":"Bowman, JL"},{"full_name":"Petrášek, J","first_name":"J","last_name":"Petrášek"}],"year":"2025","month":"05","status":"public","scopus_import":"1","quality_controlled":"1","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","file":[{"relation":"main_file","access_level":"open_access","file_name":"2025_NewPhytologist_Kurtovic.pdf","date_updated":"2025-04-16T08:03:36Z","date_created":"2025-04-16T08:03:36Z","file_id":"19571","creator":"dernst","content_type":"application/pdf","checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","success":1,"file_size":12841729}],"OA_place":"publisher","ddc":["580"],"oa_version":"Published Version","external_id":{"isi":["001438711600001"],"pmid":["40047465"]},"file_date_updated":"2025-04-16T08:03:36Z","doi":"10.1111/nph.70019","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","publication":"New Phytologist","pmid":1,"article_processing_charge":"Yes (via OA deal)"},{"article_processing_charge":"Yes (in subscription journal)","pmid":1,"publication":"Nature Plants","acknowledgement":"We thank S. Woudenberg, S. Valk and J. Rienstra for help and advice, A. Kuhn for comments on the paper and M. Prigge and M. Estelle for helpful discussions. This work was supported by a grant from Netherlands Organization for Scientific Research (NWO; OCENW.M20.031 to J.W.B.), a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2020 contract number to J.H.G.) and a research grant from the Human Frontiers Research Program (HFSP; grant RGP0015/2022 to D.W.).","external_id":{"pmid":["40216983"]},"oa_version":"Published Version","file_date_updated":"2025-12-30T07:28:09Z","doi":"10.1038/s41477-025-01975-1","ddc":["580"],"file":[{"relation":"main_file","success":1,"checksum":"8225c1899bb2f39f9a1707cc0697a052","file_size":7062474,"creator":"dernst","content_type":"application/pdf","file_id":"20882","date_updated":"2025-12-30T07:28:09Z","date_created":"2025-12-30T07:28:09Z","access_level":"open_access","file_name":"2025_NaturePlants_deRoij.pdf"}],"OA_place":"publisher","title":"ARF degradation defines a deeply conserved step in auxin response","quality_controlled":"1","scopus_import":"1","status":"public","month":"04","year":"2025","author":[{"first_name":"Martijn","last_name":"De Roij","full_name":"De Roij, Martijn"},{"full_name":"Hernández García, Jorge","first_name":"Jorge","last_name":"Hernández García"},{"id":"b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be","full_name":"Das, Shubhajit","last_name":"Das","first_name":"Shubhajit"},{"last_name":"Borst","first_name":"Jan Willem","full_name":"Borst, Jan Willem"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"}],"intvolume":" 11","publication_identifier":{"eissn":["2055-0278"]},"date_created":"2025-04-20T22:01:28Z","department":[{"_id":"JiFr"}],"publication_status":"published","date_published":"2025-04-11T00:00:00Z","type":"journal_article","volume":11,"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"In land plants, the signalling molecule auxin profoundly controls growth and development, chiefly through a transcriptional response system. The auxin response is mediated by modulating the activity of DNA-binding auxin response factor (ARF) proteins. The concentrations and stoichiometry of the competing A- and B-class ARFs define cells’ capacity for auxin response. In the minimal auxin response system of the liverwort Marchantia polymorpha, both A- and B-ARFs are unstable, but the underlying mechanisms, developmental relevance and evolutionary history of this instability are unknown. Here we identify a minimal motif that is necessary for MpARF2 (B-class) degradation and show that it is critical for development and the auxin response. Through comparative analysis and motif swaps among all ARF classes in extant algae and land plants, we infer that the emergence of ARF instability probably occurred in the ancestor of the A- and B-ARF clades and, therefore, preceded or coincided with the origin of the auxin response system.","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2025-12-30T07:28:49Z","citation":{"mla":"De Roij, Martijn, et al. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants, vol. 11, Springer Nature, 2025, pp. 717–24, doi:10.1038/s41477-025-01975-1.","ama":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 2025;11:717-724. doi:10.1038/s41477-025-01975-1","short":"M. De Roij, J. Hernández García, S. Das, J.W. Borst, D. Weijers, Nature Plants 11 (2025) 717–724.","chicago":"De Roij, Martijn, Jorge Hernández García, Shubhajit Das, Jan Willem Borst, and Dolf Weijers. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01975-1.","ieee":"M. De Roij, J. Hernández García, S. Das, J. W. Borst, and D. Weijers, “ARF degradation defines a deeply conserved step in auxin response,” Nature Plants, vol. 11. Springer Nature, pp. 717–724, 2025.","apa":"De Roij, M., Hernández García, J., Das, S., Borst, J. W., & Weijers, D. (2025). ARF degradation defines a deeply conserved step in auxin response. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01975-1","ista":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. 2025. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 11, 717–724."},"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","article_type":"letter_note","_id":"19601","publisher":"Springer Nature","day":"11","page":"717-724","OA_type":"hybrid"},{"publisher":"National Academy of Sciences","day":"20","_id":"19728","article_type":"original","OA_type":"hybrid","language":[{"iso":"eng"}],"abstract":[{"text":"Root system integrates multiple environmental cues, chiefly gravity and soil humidity, to anchor plants in soil and forage for water. While the mechanism of auxin-mediated root gravitropism is comparably well-understood, the root’s capability to grow toward moist soil for water uptake and drought avoidance, termed root hydrotropism, remains largely mysterious. Here, we provide key insights into the mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism, MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation of antagonistically acting gravitropism, which is inhibited under drought conditions. Drought stress interferes with subcellular trafficking and the lateral mobility of PIN auxin transporters, which are polarly localized at the root cell plasma membranes. This leads to defects in PIN2 polarity and gravity-induced polarization of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending. The miz1 mutant is defective in all these regulations, and in support of MIZ1’s action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant. These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism to promote root hydrotropism for efficient water foraging under drought conditions.","lang":"eng"}],"date_updated":"2026-02-16T12:30:40Z","volume":122,"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"oa":1,"has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_number":"e2427315122","issue":"20","citation":{"chicago":"Zhang, Yuzhou, Zhulatai Bao, Adrijana Smoljan, Yifan Liu, Huihui Wang, and Jiří Friml. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2427315122.","ieee":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, and J. Friml, “Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism,” Proceedings of the National Academy of Sciences, vol. 122, no. 20. National Academy of Sciences, 2025.","apa":"Zhang, Y., Bao, Z., Smoljan, A., Liu, Y., Wang, H., & Friml, J. (2025). Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2427315122","ista":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. 2025. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 122(20), e2427315122.","mla":"Zhang, Yuzhou, et al. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” Proceedings of the National Academy of Sciences, vol. 122, no. 20, e2427315122, National Academy of Sciences, 2025, doi:10.1073/pnas.2427315122.","ama":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 2025;122(20). doi:10.1073/pnas.2427315122","short":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, J. Friml, Proceedings of the National Academy of Sciences 122 (2025)."},"intvolume":" 122","publication_identifier":{"eissn":["1091-6490"]},"author":[{"first_name":"Yuzhou","last_name":"Zhang","orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Bao, Zhulatai","first_name":"Zhulatai","last_name":"Bao"},{"first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana"},{"first_name":"Yifan","last_name":"Liu","full_name":"Liu, Yifan"},{"full_name":"Wang, Huihui","last_name":"Wang","first_name":"Huihui"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2025-05-25T22:16:43Z","isi":1,"month":"05","status":"public","year":"2025","date_published":"2025-05-20T00:00:00Z","ec_funded":1,"corr_author":"1","department":[{"_id":"JiFr"}],"publication_status":"published","pmid":1,"publication":"Proceedings of the National Academy of Sciences","acknowledgement":"This work was supported by the European Union’s Horizon 2020 research and innovation Programme (European Research Council grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), (Institute of Science and Technology Austria) Fellow program, the Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program (QCYRCXM-2022-237), the Fundamental Research Funds for Northwest A&F University and partly supported by the open funds of the State Key Laboratory of Plant Environmental Resilience (SKLPERKF2416). We also thank the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly Dr. Ningjuan Fan for technical assistance.","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","title":"Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism","scopus_import":"1","ddc":["580"],"file_date_updated":"2025-05-28T08:04:50Z","external_id":{"pmid":["40372432"],"isi":["001496347500001"]},"doi":"10.1073/pnas.2427315122","oa_version":"Published Version","OA_place":"publisher","file":[{"relation":"main_file","date_updated":"2025-05-28T08:04:50Z","date_created":"2025-05-28T08:04:50Z","access_level":"open_access","file_name":"2025_PNAS_Zhang.pdf","checksum":"f70ff35054561b27a463ba279d1795dc","success":1,"file_size":8266672,"file_id":"19750","creator":"dernst","content_type":"application/pdf"}]},{"day":"19","publisher":"Springer Nature","article_type":"review","_id":"19736","OA_type":"closed access","date_updated":"2025-09-30T12:41:30Z","language":[{"iso":"eng"}],"abstract":[{"text":"The phytohormone auxin is a major signal coordinating growth and development in plants. The variety of its effects arises from its ability to form local auxin maxima and gradients within tissues, generated through directional cell-to-cell transport and elaborate metabolic control. These auxin distribution patterns instruct cells in a context-dependent manner to undergo predefined developmental transitions. In this Review, we discuss advances in auxin action at the level of homeostasis and signalling. We highlight key insights into the structural basis of PIN-mediated intercellular auxin transport and explore two novel non-transcriptional auxin signalling mechanisms: one involving intracellular Ca2+ transients and another involving cell-surface auxin perception that mediates global, ultrafast phosphorylation. Furthermore, we examine emerging evidence indicating the involvement of cyclic adenosine monophosphate as a second messenger in the transcriptional auxin response. Together, these recent developments in auxin research have profoundly deepened our understanding of the complex and diverse activities of auxin in plant growth and development.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","article_number":"e113018","citation":{"apa":"Vanneste, S., Pei, Y., & Friml, J. (2025). Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. Springer Nature. https://doi.org/10.1038/s41580-025-00851-2","chicago":"Vanneste, Steffen, Yuanrong Pei, and Jiří Friml. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology. Springer Nature, 2025. https://doi.org/10.1038/s41580-025-00851-2.","ieee":"S. Vanneste, Y. Pei, and J. Friml, “Mechanisms of auxin action in plant growth and development,” Nature Reviews Molecular Cell Biology. Springer Nature, 2025.","ista":"Vanneste S, Pei Y, Friml J. 2025. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology., e113018.","mla":"Vanneste, Steffen, et al. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology, e113018, Springer Nature, 2025, doi:10.1038/s41580-025-00851-2.","short":"S. Vanneste, Y. Pei, J. Friml, Nature Reviews Molecular Cell Biology (2025).","ama":"Vanneste S, Pei Y, Friml J. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. 2025. doi:10.1038/s41580-025-00851-2"},"date_created":"2025-05-25T22:16:57Z","isi":1,"publication_identifier":{"eissn":["1471-0080"],"issn":["1471-0072"]},"author":[{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"last_name":"Pei","first_name":"Yuanrong","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","full_name":"Pei, Yuanrong"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"year":"2025","month":"05","status":"public","corr_author":"1","date_published":"2025-05-19T00:00:00Z","publication_status":"published","department":[{"_id":"JiFr"}],"pmid":1,"publication":"Nature Reviews Molecular Cell Biology","article_processing_charge":"No","scopus_import":"1","quality_controlled":"1","title":"Mechanisms of auxin action in plant growth and development","doi":"10.1038/s41580-025-00851-2","oa_version":"None","external_id":{"pmid":["40389696"],"isi":["001490500500001"]}}]