[{"OA_type":"green","date_created":"2026-06-13T16:57:07Z","status":"public","publication":"bioRxiv","ddc":["580"],"year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"30","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"type":"preprint","publication_status":"submitted","OA_place":"repository","language":[{"iso":"eng"}],"oa":1,"date_updated":"2026-06-19T07:14:01Z","month":"05","acknowledgement":"We would like to thank Dr. Yvon Jaillais (ENS, Lyon) for sharing MAKR2 materials. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The research in the Friml group leading to these results was funded by the European Research Council (ERC): 101142681 CYNIPS; and the Austrian Science Fund (FWF): I 6123-B and P 37051-B. Ewa Mazur was supported by the National Science Centre (NCN), Poland, under the OPUS call in the WEAVE programme: 2021/43/I/NZ1/01835.","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"scopus_import":"1","corr_author":"1","doi":"10.1101/2025.10.07.680881","main_file_link":[{"url":"https://doi.org/10.1101/2025.10.07.680881","open_access":"1"}],"has_accepted_license":"1","author":[{"first_name":"Zengxiang","orcid":"0000-0001-9381-3577","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge","full_name":"Ge, Zengxiang"},{"first_name":"Lilla","last_name":"Koczka","full_name":"Koczka, Lilla"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","first_name":"Gergely"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"first_name":"Nada","full_name":"Kassem, Nada","last_name":"Kassem"},{"first_name":"Sara","id":"6a0bb896-6bad-11f1-9bef-906e9eb76034","last_name":"Ait Ikene","full_name":"Ait Ikene, Sara"},{"full_name":"Fiedler, Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","first_name":"Lukas"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"}],"abstract":[{"text":"Adaptive plant development is orchestrated, among others, by directional, intercellular transport of the phytohormone auxin. Self-organizing development, such as flexible vasculature formation, depends on so-called auxin canalization, manifested by the gradual formation of auxin transport channels through feedback between auxin signalling and transport. Herein, we identify MAKR6 as an important, novel component in this feedback. MAKR6 expression accumulates strongly in vascular cells and is tightly regulated by auxin via the Aux/IAA-ARF-WRKY23 transcriptional network. MAKR6 is required for auxin canalization-dependent processes, including leaf venation, vasculature regeneration, and de novo auxin channel formation from local auxin sources. Mechanistically, MAKR6 interacts with the PIN1 auxin transporter, modulating its trafficking and polarization. MAKR6 also associates with and integrates two key receptor-like kinase complexes involved in canalization, TMK1/4 and the CAMEL-CANAR. Together, our study establishes MAKR6 as a multifaceted regulator that couples transcriptional auxin signalling to PIN1 repolarization and coordinates multiple RLK-mediated signalling pathways during canalization. This provides mechanistic insights into auxin canalization and exemplifies a framework for exploring similar regulatory nodes in other developmental contexts.","lang":"eng"}],"title":"MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis","project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"oa_version":"Preprint","article_processing_charge":"No","_id":"21994","citation":{"ama":"Ge Z, Koczka L, Mazur E, et al. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv. doi:10.1101/2025.10.07.680881","apa":"Ge, Z., Koczka, L., Mazur, E., Molnar, G., Vladimirtsev, D., Kassem, N., … Friml, J. (n.d.). MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv. https://doi.org/10.1101/2025.10.07.680881","ista":"Ge Z, Koczka L, Mazur E, Molnar G, Vladimirtsev D, Kassem N, Ait Ikene S, Fiedler L, Friml J. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv, 10.1101/2025.10.07.680881.","mla":"Ge, Zengxiang, et al. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” BioRxiv, doi:10.1101/2025.10.07.680881.","ieee":"Z. Ge et al., “MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis,” bioRxiv. .","chicago":"Ge, Zengxiang, Lilla Koczka, Ewa Mazur, Gergely Molnar, Dmitrii Vladimirtsev, Nada Kassem, Sara Ait Ikene, Lukas Fiedler, and Jiří Friml. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.07.680881.","short":"Z. Ge, L. Koczka, E. Mazur, G. Molnar, D. Vladimirtsev, N. Kassem, S. Ait Ikene, L. Fiedler, J. Friml, BioRxiv (n.d.)."},"date_published":"2026-05-30T00:00:00Z"},{"title":"Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels","author":[{"full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","first_name":"Dmitrii"}],"has_accepted_license":"1","doi":"10.15479/AT-ISTA-20964","corr_author":"1","publisher":"Institute of Science and Technology Austria","date_published":"2026-01-14T00:00:00Z","publication_identifier":{"issn":["2791-4585"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"citation":{"ista":"Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria.","ama":"Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:10.15479/AT-ISTA-20964","apa":"Vladimirtsev, D. (2026). Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20964","mla":"Vladimirtsev, Dmitrii. Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-20964.","ieee":"D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026.","chicago":"Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-20964.","short":"D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026."},"_id":"20964","degree_awarded":"MS","article_processing_charge":"No","oa_version":"Published Version","file_date_updated":"2026-01-28T12:38:19Z","supervisor":[{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"page":"22","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"}],"ddc":["570"],"alternative_title":["ISTA Master’s Thesis"],"related_material":{"record":[{"id":"20982","status":"public","relation":"part_of_dissertation"}]},"status":"public","date_created":"2026-01-09T09:22:48Z","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"date_updated":"2026-04-07T11:41:44Z","month":"01","language":[{"iso":"eng"}],"OA_place":"publisher","file":[{"creator":"dvladimi","relation":"main_file","embargo_to":"open_access","checksum":"812857b2fbe3f6113bef22fd04bccd3e","embargo":"2027-01-01","date_updated":"2026-01-21T14:12:13Z","file_size":2867531,"access_level":"closed","file_name":"2026_Vladimirtsev_Dmitrii_Thesis.pdf","content_type":"application/pdf","file_id":"21033","date_created":"2026-01-21T14:12:13Z"},{"file_size":25023066,"date_updated":"2026-01-28T12:38:19Z","access_level":"closed","creator":"dvladimi","relation":"source_file","checksum":"2b969f97f8d7461bea3d255f48c2219c","date_created":"2026-01-21T14:41:58Z","file_name":"Source Files.zip","file_id":"21034","content_type":"application/x-zip-compressed"}],"publication_status":"published","type":"dissertation","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","day":"14","year":"2026"},{"oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","publisher":"Wiley","date_published":"2026-03-11T00:00:00Z","citation":{"ista":"Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist., nph. 71072.","ama":"Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 2026. doi:10.1111/nph.71072","apa":"Babic, D., Zupunski, M., & Friml, J. (2026). Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. Wiley. https://doi.org/10.1111/nph.71072","mla":"Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist, nph. 71072, Wiley, 2026, doi:10.1111/nph.71072.","ieee":"D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study Arabidopsis embryogenesis,” New Phytologist. Wiley, 2026.","chicago":"Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist. Wiley, 2026. https://doi.org/10.1111/nph.71072.","short":"D. Babic, M. Zupunski, J. Friml, New Phytologist (2026)."},"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"_id":"21483","corr_author":"1","quality_controlled":"1","external_id":{"pmid":["41808651"]},"author":[{"last_name":"Babic","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","full_name":"Babic, David","first_name":"David"},{"first_name":"Milan","id":"f6a21fce-573e-11f0-a150-a8d96aee2539","last_name":"Zupunski","full_name":"Zupunski, Milan"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"has_accepted_license":"1","doi":"10.1111/nph.71072","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.71072"}],"abstract":[{"lang":"eng","text":"Embryogenesis in the model plant Arabidopsis thaliana provides a framework for understanding how cell polarity and patterning coordinate with hormonal signalling to establish the plant body plan. Following fertilisation, the zygote divides asymmetrically to generate apical and basal lineages, establishing the apical–basal axis that defines future shoot and root poles. Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical–basal axis establishment and organ initiation. The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors. Advances in microscopy have transformed the study of Arabidopsis embryogenesis: fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation, and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development, while laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity. Together, these methodological breakthroughs position Arabidopsis embryos as a prime model for dissecting the chemical and biophysical cues that shape plant development."}],"title":"Imaging and genetic toolbox to study Arabidopsis embryogenesis","article_type":"original","publication_status":"epub_ahead","type":"journal_article","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","day":"11","OA_place":"publisher","acknowledgement":"The authors would like to acknowledge the many colleagues whose valuable contributions to the field could not be included in this review due to space limitations and reference constraints. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ.","language":[{"iso":"eng"}],"month":"03","date_updated":"2026-06-18T08:31:45Z","oa":1,"pmid":1,"department":[{"_id":"JiFr"},{"_id":"GradSch"}],"publication":"New Phytologist","status":"public","date_created":"2026-03-23T14:59:06Z","OA_type":"hybrid","ddc":["580"],"PlanS_conform":"1","article_number":"nph.71072"},{"acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","oa":1,"month":"03","language":[{"iso":"eng"}],"date_updated":"2026-03-24T08:36:40Z","pmid":1,"department":[{"_id":"JiFr"}],"type":"journal_article","publication_status":"published","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","day":"23","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"OA_place":"publisher","file":[{"checksum":"fe6c41fdab58a55df5f2a5860c02acdc","creator":"dernst","relation":"main_file","file_size":12986894,"date_updated":"2026-03-24T08:34:37Z","access_level":"open_access","content_type":"application/pdf","file_id":"21496","success":1,"file_name":"2026_CurrentBiology_Li.pdf","date_created":"2026-03-24T08:34:37Z"}],"status":"public","publication":"Current Biology","OA_type":"hybrid","date_created":"2026-03-23T15:11:16Z","ddc":["580"],"PlanS_conform":"1","volume":36,"article_processing_charge":"Yes (via OA deal)","citation":{"ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023","apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6."},"date_published":"2026-03-23T00:00:00Z","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"publication_identifier":{"issn":["0960-9822"]},"publisher":"Elsevier","_id":"21490","project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"bd906599-d553-11ed-ba76-abf8547645d7","name":"Identification of a novel regulator in auxin canalization","grant_number":"E271"}],"page":"1468-1480.e6","file_date_updated":"2026-03-24T08:34:37Z","intvolume":" 36","oa_version":"Published Version","author":[{"first_name":"Mingyue","last_name":"Li","id":"01f96916-0235-11eb-9379-a323192643b7","full_name":"Li, Mingyue"},{"first_name":"Nikola","full_name":"Rydza, Nikola","last_name":"Rydza"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"first_name":"Gergely","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"has_accepted_license":"1","doi":"10.1016/j.cub.2026.02.023","abstract":[{"text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development.","lang":"eng"}],"title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","corr_author":"1","quality_controlled":"1","issue":"6","external_id":{"pmid":["41831441"]}},{"ddc":["580"],"publication":"Science","status":"public","date_created":"2026-04-26T22:01:47Z","OA_type":"green","OA_place":"repository","file":[{"date_created":"2026-05-07T05:54:43Z","file_name":"2026_Science_Kulich_accepted.pdf","success":1,"file_id":"21832","content_type":"application/pdf","file_size":6150733,"date_updated":"2026-05-07T05:54:43Z","access_level":"open_access","relation":"main_file","creator":"dernst","checksum":"eb5b29247832ecdc53c8146da0509bbe"}],"publication_status":"published","article_type":"original","type":"journal_article","day":"16","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"department":[{"_id":"JiFr"},{"_id":"GradSch"}],"acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","month":"04","language":[{"iso":"eng"}],"oa":1,"date_updated":"2026-05-07T06:20:07Z","external_id":{"pmid":["41990180"]},"corr_author":"1","scopus_import":"1","quality_controlled":"1","issue":"6795","title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","abstract":[{"text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.","lang":"eng"}],"has_accepted_license":"1","author":[{"full_name":"Kulich, Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","first_name":"Ivan"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek"},{"first_name":"Shiqiang","full_name":"Gao, Shiqiang","last_name":"Gao"},{"first_name":"Matteo","full_name":"Citterico, Matteo","last_name":"Citterico"},{"first_name":"Kai R.","last_name":"Konrad","full_name":"Konrad, Kai R."},{"last_name":"Nagel","full_name":"Nagel, Georg","first_name":"Georg"},{"last_name":"Wrzaczek","full_name":"Wrzaczek, Michael","first_name":"Michael"},{"last_name":"Cascaro","full_name":"Cascaro, Léa","first_name":"Léa"},{"first_name":"Pauline","full_name":"Vinet, Pauline","last_name":"Vinet"},{"full_name":"Durand, Pauline","last_name":"Durand","first_name":"Pauline"},{"full_name":"Asnacios, Atef","last_name":"Asnacios","first_name":"Atef"},{"first_name":"Lokesh","full_name":"Verma, Lokesh","last_name":"Verma"},{"first_name":"Malcolm J.","last_name":"Bennett","full_name":"Bennett, Malcolm J."},{"first_name":"Bipin K.","full_name":"Pandey, Bipin K.","last_name":"Pandey"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"doi":"10.1126/science.adu8197","intvolume":" 392","oa_version":"Accepted Version","file_date_updated":"2026-05-07T05:54:43Z","page":"296-300","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"}],"publisher":"AAAS","date_published":"2026-04-16T00:00:00Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"citation":{"ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 2026;392(6795):296-300. doi:10.1126/science.adu8197","apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. AAAS. https://doi.org/10.1126/science.adu8197","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” Science, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:10.1126/science.adu8197.","ieee":"I. Kulich et al., “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” Science, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” Science. AAAS, 2026. https://doi.org/10.1126/science.adu8197.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300."},"_id":"21763","volume":392,"article_processing_charge":"No"},{"pmid":1,"department":[{"_id":"JiFr"}],"acknowledgement":" We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-HC2022/02 and Max-Planck-Society to A.S. ","oa":1,"month":"05","language":[{"iso":"eng"}],"date_updated":"2026-06-02T14:36:41Z","OA_place":"publisher","file":[{"access_level":"open_access","date_updated":"2026-06-02T14:33:55Z","file_size":2014452,"checksum":"75b8ef2db078652c750e34e9cd98a808","creator":"dernst","relation":"main_file","date_created":"2026-06-02T14:33:55Z","content_type":"application/pdf","file_id":"21941","success":1,"file_name":"2026_ScienceAdv_Li2.pdf"}],"publication_status":"published","article_type":"original","type":"journal_article","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"day":"08","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","article_number":"aea7828","ddc":["580"],"PlanS_conform":"1","publication":"Science Advances","status":"public","OA_type":"gold","date_created":"2026-05-24T22:01:31Z","publisher":"AAAS","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"LifeSc"}],"publication_identifier":{"eissn":["2375-2548"]},"date_published":"2026-05-08T00:00:00Z","citation":{"ista":"Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828.","apa":"Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.aea7828","ama":"Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 2026;12(19). doi:10.1126/sciadv.aea7828","short":"M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026).","ieee":"M. Li et al., “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” Science Advances, vol. 12, no. 19. AAAS, 2026.","chicago":"Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” Science Advances. AAAS, 2026. https://doi.org/10.1126/sciadv.aea7828.","mla":"Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” Science Advances, vol. 12, no. 19, aea7828, AAAS, 2026, doi:10.1126/sciadv.aea7828."},"_id":"21914","volume":12,"article_processing_charge":"Yes","oa_version":"Published Version","intvolume":" 12","file_date_updated":"2026-06-02T14:33:55Z","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"title":"Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants","abstract":[{"lang":"eng","text":"Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology."}],"has_accepted_license":"1","author":[{"first_name":"Mingyue","full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","last_name":"Li"},{"full_name":"Chodasiewicz, Monika","last_name":"Chodasiewicz","first_name":"Monika"},{"first_name":"Malavika","last_name":"Muraleedharan","full_name":"Muraleedharan, Malavika"},{"first_name":"Israel M.","last_name":"Lopez","full_name":"Lopez, Israel M."},{"full_name":"Gorka, Michal","last_name":"Gorka","first_name":"Michal"},{"last_name":"Kerber","full_name":"Kerber, Olga","first_name":"Olga"},{"first_name":"Saqer S.","last_name":"Alotaibi","full_name":"Alotaibi, Saqer S."},{"first_name":"Andrew D.L.","last_name":"Nelson","full_name":"Nelson, Andrew D.L."},{"full_name":"Lenobel, Rene","last_name":"Lenobel","first_name":"Rene"},{"first_name":"Jaroslava","full_name":"Friedecká, Jaroslava","last_name":"Friedecká"},{"full_name":"Skirycz, Aleksandra","last_name":"Skirycz","first_name":"Aleksandra"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"DOAJ_listed":"1","doi":"10.1126/sciadv.aea7828","external_id":{"pmid":["42102187"]},"corr_author":"1","quality_controlled":"1","scopus_import":"1","issue":"19"},{"article_processing_charge":"No","language":[{"iso":"eng"}],"month":"04","date_updated":"2026-07-13T12:25:19Z","das_tickbox":"1","department":[{"_id":"JiFr"}],"_id":"22293","publisher":"Springer Nature","editor":[{"first_name":"Fang ","last_name":"Chang","full_name":"Chang, Fang "},{"first_name":"Yingxiang","full_name":"Wang, Yingxiang","last_name":"Wang"},{"full_name":"Ma, Hong","last_name":"Ma","first_name":"Hong"}],"citation":{"mla":"Zhong, Sheng, et al. “Pollination and Fertilization.” Regulation of Plant Development, edited by Fang Chang et al., Springer Nature, 2026, pp. 537–615, doi:10.1007/978-981-95-7033-1_14.","short":"S. Zhong, Z. Lan, Z. Ge, L.-J. Qu, in:, F. Chang, Y. Wang, H. Ma (Eds.), Regulation of Plant Development, Springer Nature, Singapore, 2026, pp. 537–615.","chicago":"Zhong, Sheng, Zijun Lan, Zengxiang Ge, and Li-Jia Qu. “Pollination and Fertilization.” In Regulation of Plant Development, edited by Fang Chang, Yingxiang Wang, and Hong Ma, 537–615. Singapore: Springer Nature, 2026. https://doi.org/10.1007/978-981-95-7033-1_14.","ieee":"S. Zhong, Z. Lan, Z. Ge, and L.-J. Qu, “Pollination and Fertilization,” in Regulation of Plant Development, F. Chang, Y. Wang, and H. Ma, Eds. Singapore: Springer Nature, 2026, pp. 537–615.","ista":"Zhong S, Lan Z, Ge Z, Qu L-J. 2026.Pollination and Fertilization. In: Regulation of Plant Development. , 537–615.","ama":"Zhong S, Lan Z, Ge Z, Qu L-J. Pollination and Fertilization. In: Chang F, Wang Y, Ma H, eds. Regulation of Plant Development. Singapore: Springer Nature; 2026:537-615. doi:10.1007/978-981-95-7033-1_14","apa":"Zhong, S., Lan, Z., Ge, Z., & Qu, L.-J. (2026). Pollination and Fertilization. In F. Chang, Y. Wang, & H. Ma (Eds.), Regulation of Plant Development (pp. 537–615). Singapore: Springer Nature. https://doi.org/10.1007/978-981-95-7033-1_14"},"publication_identifier":{"eisbn":["9789819570331"],"isbn":["9789819570324"]},"date_published":"2026-04-24T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","day":"24","page":"537-615","publication_status":"published","type":"book_chapter","oa_version":"None","doi":"10.1007/978-981-95-7033-1_14","author":[{"last_name":"Zhong","full_name":"Zhong, Sheng","first_name":"Sheng"},{"last_name":"Lan","full_name":"Lan, Zijun","first_name":"Zijun"},{"first_name":"Zengxiang","orcid":"0000-0001-9381-3577","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge","full_name":"Ge, Zengxiang"},{"full_name":"Qu, Li-Jia","last_name":"Qu","first_name":"Li-Jia"}],"title":"Pollination and Fertilization","abstract":[{"lang":"eng","text":"In order to cope with arid terrestrial environments, angiosperms have evolved a unique fertilization way—siphonogamy. The success of siphonogamy requires several prerequisites, including the normal development of the female gametophyte and male gametophyte (pollen). Appropriate pollination methods ensure the successful encounter between pollen and the stigma. After pollen lands on the stigma, pollen/pollen tube interacts with different tissues and cells of the pistil, completing a series of male-female communications. The smooth progress of these interactions ensures that the pollen tube can enter the female gametophyte, burst, and release sperm cells to complete the double fertilization. In this chapter, we provide an overview of pollination and the interactions between male and female, comprehensively summarizing the factors involved in these processes."}],"OA_type":"closed access","date_created":"2026-07-13T09:46:18Z","quality_controlled":"1","scopus_import":"1","publication":"Regulation of Plant Development","status":"public","place":"Singapore"},{"project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","supplementarymaterial":"yes","_id":"22301","publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"citation":{"ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026. doi:10.1111/jipb.70309","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026, doi:10.1111/jipb.70309.","ieee":"A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte algae,” Journal of Integrative Plant Biology. Wiley, 2026.","chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology. Wiley, 2026. https://doi.org/10.1111/jipb.70309.","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026)."},"date_published":"2026-06-10T00:00:00Z","publisher":"Wiley","quality_controlled":"1","scopus_import":"1","corr_author":"1","external_id":{"pmid":["42271607"]},"doi":"10.1111/jipb.70309","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/jipb.70309"}],"author":[{"first_name":"Adrijana","full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan"},{"full_name":"Koutnik‐Abele, Sarah","last_name":"Koutnik‐Abele","first_name":"Sarah"},{"full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","first_name":"Dmitrii"},{"first_name":"Petr","full_name":"Klíma, Petr","last_name":"Klíma"},{"last_name":"Bírošíková","full_name":"Bírošíková, Anita","first_name":"Anita"},{"full_name":"Zhang, Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"37e65def-d415-11eb-ae59-a7b67be103db","last_name":"Schuster","full_name":"Schuster, Maximilian","first_name":"Maximilian"},{"first_name":"Katarina","full_name":"Kurtović, Katarina","last_name":"Kurtović"},{"first_name":"Ulrich Z.","last_name":"Hammes","full_name":"Hammes, Ulrich Z."},{"last_name":"Petrášek","full_name":"Petrášek, Jan","first_name":"Jan"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"has_accepted_license":"1","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","abstract":[{"lang":"eng","text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","day":"10","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","publication_status":"epub_ahead","article_type":"original","OA_place":"publisher","month":"06","language":[{"iso":"eng"}],"oa":1,"date_updated":"2026-07-13T14:26:31Z","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","das_tickbox":"0","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"pmid":1,"OA_type":"hybrid","date_created":"2026-07-13T10:44:55Z","status":"public","publication":"Journal of Integrative Plant Biology","PlanS_conform":"1","ddc":["580"],"researchdata_availability":"no","article_number":"jipb.70309"},{"scopus_import":"1","quality_controlled":"1","issue":"6807","external_id":{"pmid":["42424472"]},"author":[{"first_name":"Zhulatai","full_name":"Bao, Zhulatai","last_name":"Bao"},{"full_name":"Wang, Huihui","last_name":"Wang","first_name":"Huihui"},{"last_name":"Zhang","full_name":"Zhang, Ai","first_name":"Ai"},{"first_name":"Ruxi","full_name":"Gao, Ruxi","last_name":"Gao"},{"first_name":"Wen","last_name":"Gu","full_name":"Gu, Wen"},{"first_name":"Ni","last_name":"Fan","full_name":"Fan, Ni"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Yuzhou","full_name":"Zhang, Yuzhou","last_name":"Zhang"}],"doi":"10.1126/science.adw6568","title":"Roots navigate around decay regions by sensing local pH gradients","abstract":[{"text":"Plant tropisms enable roots to navigate complex soils by responding to directional environmental cues. Biological decay, although central to nutrient cycling, also creates microbially active and potentially hostile niches. In this work, we identified “saprotropism,” a previously unrecognized growth response that enables roots to actively bend away from decaying plant-derived matter. Fungal-driven microbial decomposition released organic acids and formed stable pH gradients in surrounding soil, allowing roots to pinpoint decay without direct contact. Root epidermal cells sensed this acidic gradient through the root meristem growth factor peptide-receptor module, converting external pH asymmetry into asymmetric abscisic acid (ABA) distribution. ABA asymmetry drove microtubule reorganization, which was decoded into decay-avoidant root bending. Together, these findings establish microbial decay–derived chemical gradients as an instructive signal for root navigation and expand the framework of microbe-soil-plant communication.","lang":"eng"}],"project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"}],"oa_version":"None","intvolume":" 393","volume":393,"article_processing_charge":"No","publisher":"American Association for the Advancement of Science","citation":{"apa":"Bao, Z., Wang, H., Zhang, A., Gao, R., Gu, W., Fan, N., … Zhang, Y. (2026). Roots navigate around decay regions by sensing local pH gradients. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.adw6568","ama":"Bao Z, Wang H, Zhang A, et al. Roots navigate around decay regions by sensing local pH gradients. Science. 2026;393(6807). doi:10.1126/science.adw6568","ista":"Bao Z, Wang H, Zhang A, Gao R, Gu W, Fan N, Friml J, Zhang Y. 2026. Roots navigate around decay regions by sensing local pH gradients. Science. 393(6807), eadw6568.","ieee":"Z. Bao et al., “Roots navigate around decay regions by sensing local pH gradients,” Science, vol. 393, no. 6807. American Association for the Advancement of Science, 2026.","chicago":"Bao, Zhulatai, Huihui Wang, Ai Zhang, Ruxi Gao, Wen Gu, Ni Fan, Jiří Friml, and Yuzhou Zhang. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” Science. American Association for the Advancement of Science, 2026. https://doi.org/10.1126/science.adw6568.","short":"Z. Bao, H. Wang, A. Zhang, R. Gao, W. Gu, N. Fan, J. Friml, Y. Zhang, Science 393 (2026).","mla":"Bao, Zhulatai, et al. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” Science, vol. 393, no. 6807, eadw6568, American Association for the Advancement of Science, 2026, doi:10.1126/science.adw6568."},"date_published":"2026-07-09T00:00:00Z","publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"_id":"22315","supplementarymaterial":"yes","publication":"Science","status":"public","OA_type":"closed access","date_created":"2026-07-13T14:57:10Z","article_number":"eadw6568","researchdata_availability":"yes","article_type":"original","publication_status":"published","type":"journal_article","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"09","das_tickbox":"1","acknowledgement":"We are grateful to H. Guo and L. Liu (Department of Biology, Southern University of Science and Technology) for providing the rgf1/2/3, rgi1/2/3/4, tpst-1, and pepr1/2 lines. We thank K.-h. Liu (College of Life Science, Northwest A&F University) for generously providing the ABA biosensor nlsABACUS2-400n. We also thank J. Li and J. Chang (School of Life Sciences, Lanzhou University) for providing the ahk2-5/cre1-2, ahp1/2/3, arr16/arr17, and pTCSn::GFP lines. Our thanks further extend to D. Qian, also from the School of Life Sciences at Lanzhou University, for sharing Arabidopsis line pTUB6::mCherry-TUB6. We are grateful to Y. Zhao (CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences) for providing nced3/5, snrk2.2/2.3/2.6, and pyl duodecuple mutants. We also acknowledge the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly N. Fan, for their invaluable technical assistance. We also thank Life Science Research Core Services (LSRCS), Northwest A&F University, for helping with characterization, including CLSM (X. Liu). Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China JYB2025XDXM706 (Y.Z.); Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program QCYRCXM-2022-237 (Y.Z.); Fundamental Research Funds for the Central Universities K20200168 (Y.Z.); National Natural Science Foundation of China 32570375 (Y.Z.); National Natural Science Foundation of China 32400699 (A.Z.); European Research Council (ERC, CYNIPS) 101142681 (J.F.); Austrian Science Fund (FWF): P 37051-B (J.F.).","month":"07","language":[{"iso":"eng"}],"date_updated":"2026-07-14T08:11:55Z","dataavailabilitystatement":"All data are available in the manuscript or the supplementary materials. The raw RNA-seq data have been deposited in the NCBI Gene Expression Omnibus (GEO) under accession number GSE315473. Microbiome sequencing data have been deposited in the Sequence Read Archive (SRA) under BioProject number PRJNA1397137. Materials are available upon request from the corresponding author.","pmid":1,"department":[{"_id":"JiFr"}]},{"intvolume":" 127","oa_version":"None","article_processing_charge":"No","volume":127,"supplementarymaterial":"yes","_id":"22366","publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"date_published":"2026-07-01T00:00:00Z","citation":{"ista":"Sun L, Jia W, Mao Y, Li X, Kong M, She J, Friml J, Tan S. 2026. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 127(1), e71042.","ama":"Sun L, Jia W, Mao Y, et al. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 2026;127(1). doi:10.1111/tpj.71042","apa":"Sun, L., Jia, W., Mao, Y., Li, X., Kong, M., She, J., … Tan, S. (2026). Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. Wiley. https://doi.org/10.1111/tpj.71042","mla":"Sun, Lianghanxiao, et al. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal, vol. 127, no. 1, e71042, Wiley, 2026, doi:10.1111/tpj.71042.","chicago":"Sun, Lianghanxiao, Wenxin Jia, Yanbo Mao, Xin Li, Mengjuan Kong, Ji She, Jiří Friml, and Shutang Tan. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal. Wiley, 2026. https://doi.org/10.1111/tpj.71042.","short":"L. Sun, W. Jia, Y. Mao, X. Li, M. Kong, J. She, J. Friml, S. Tan, The Plant Journal 127 (2026).","ieee":"L. Sun et al., “Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis,” The Plant Journal, vol. 127, no. 1. Wiley, 2026."},"publisher":"Wiley","issue":"1","quality_controlled":"1","scopus_import":"1","external_id":{"pmid":["42438075"]},"doi":"10.1111/tpj.71042","author":[{"last_name":"Sun","full_name":"Sun, Lianghanxiao","first_name":"Lianghanxiao"},{"first_name":"Wenxin","full_name":"Jia, Wenxin","last_name":"Jia"},{"first_name":"Yanbo","last_name":"Mao","full_name":"Mao, Yanbo"},{"last_name":"Li","full_name":"Li, Xin","first_name":"Xin"},{"first_name":"Mengjuan","full_name":"Kong, Mengjuan","last_name":"Kong"},{"first_name":"Ji","last_name":"She","full_name":"She, Ji"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"}],"keyword":["auxin","SAC1","GRV2","PIN3","vacuole","gravitropism","Arabidopsis"],"title":"Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis","abstract":[{"lang":"eng","text":"Gravitropism is a fundamental adaptive response in plants that enables directional growth to optimize resource acquisition. In this study, we employed forward genetic screening to identify Arabidopsis mutants with defective hypocotyl gravitropism and isolated the short and agravitropic hypocotyl in dark1 (sad1) mutant, which carries a point mutation (G110E) in the SAC1 gene encoding a phosphoinositide phosphatase. Deficiency of SAC1 disrupted gravity-induced polar localization of PIN3 in endodermal cells, impairing auxin redistribution and leading to hypocotyl gravitropism defects. Subcellular localization analysis revealed that SAC1 is partially localized to the PVC/tonoplast and participates in late endosomal trafficking. The sac1 mutation leads to abnormal vacuolar morphology, which is associated with defects in amyloplast sedimentation during the gravitropic response in Arabidopsis shoots. We further revealed that SAC1 interacts with GRV2, a key regulator of the late endocytic pathway, and that both proteins cooperatively regulate shoot gravitropism. In summary, this study identified SAC1 as a regulator of shoot gravitropism, revealing its important role in modulating vacuolar homeostasis, amyloplast sedimentation, PIN3 trafficking, and auxin distribution. These findings provide insights into the molecular mechanisms linking membrane transport to environmental adaptation in plants."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","day":"01","type":"journal_article","article_type":"original","publication_status":"published","date_updated":"2026-07-20T13:53:44Z","month":"07","language":[{"iso":"eng"}],"acknowledgement":"We acknowledge Prof. Dolf Weijers (Wageningen University), Prof.Karin Schumacher (Heidelberg University), Prof. Yohann Boutt ´e(Universit ´e de Bordeaux), and Prof. Jinbo Shen (Zhejiang A&FUniversity) for providing published plasmids and Arabidopsislines. We thank Dr. Gergely Moln ´ar (ISTA) for help with NGS dataanalysis, and Prof. Jianru Zuo (IGDB, CAS), Prof. Chengbin Xiang(USTC), and Prof. Zhong Zhao (USTC) for critical comments onthe manuscript. We thank the staff members of the Mass Spec-trometry System at the National Facility for Protein Science inShanghai (NFPS), Zhangjiang Lab, China for providing technicalsupport and assistance in data collection and analysis. This workwas supported by grants from the National Natural Science Foun-dation of China (32570366, and 32321001 to ST), the Natural Sci-ence Foundation of Anhui Province (2508085QC070 to MK), theFundamental Research Funds for the Central Universities(WK9100250095 to MK, and WK9100000021 to ST), the ForestryBureau of Anhui Province (AHLYJBGS-2024-01 to ST), the Centerfor Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine, University of Science andTechnology of China (QYPY20220012 to ST), the USTC ResearchFunds of the Double First-Class Initiative (YD9100002016 to ST),and start-up funding from the University of Science and Technol-ogy of China and the Chinese Academy of Sciences(GG9100007007, KY9100000026, KY9100000051, XKTS-202591014,XKTS-2026910122, and KJ2070000079 to ST).","das_tickbox":"1","department":[{"_id":"JiFr"}],"pmid":1,"dataavailabilitystatement":"Biological materials (seeds, plasmids) are available upon request from ST (sttan@ustc.edu.cn). The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.","date_created":"2026-07-19T22:01:47Z","OA_type":"closed access","status":"public","publication":"The Plant Journal","researchdata_availability":"upon request","article_number":"e71042"},{"month":"05","language":[{"iso":"eng"}],"date_updated":"2026-04-07T11:41:43Z","oa":1,"article_processing_charge":"No","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","_id":"20982","department":[{"_id":"JiFr"}],"date_published":"2025-05-16T00:00:00Z","citation":{"ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, 10.1101/2025.01.06.631460.","apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. https://doi.org/10.1101/2025.01.06.631460","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. doi:10.1101/2025.01.06.631460","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, n.d. https://doi.org/10.1101/2025.01.06.631460.","ieee":"I. Kulich et al., “Armadillo repeat only proteins are required for the function of plant CNGC channels,” bioRxiv. .","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, doi:10.1101/2025.01.06.631460."},"day":"16","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2025","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"type":"preprint","publication_status":"draft","OA_place":"repository","oa_version":"Preprint","main_file_link":[{"url":"https://doi.org/10.1101/2025.01.06.631460","open_access":"1"}],"doi":"10.1101/2025.01.06.631460","author":[{"full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","first_name":"Ivan"},{"first_name":"Denisa","full_name":"Oulehlová, Denisa","last_name":"Oulehlová"},{"first_name":"Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii"},{"first_name":"Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia"},{"last_name":"Lileikyte","full_name":"Lileikyte, Edita","first_name":"Edita"},{"last_name":"Bondar","full_name":"Bondar, Alexey","first_name":"Alexey"},{"last_name":"Kulichová","full_name":"Kulichová, Katarína","first_name":"Katarína"},{"full_name":"Janda, Martin","last_name":"Janda","first_name":"Martin"},{"first_name":"Oksana","last_name":"Iakovenko","full_name":"Iakovenko, Oksana"},{"last_name":"Neubergerová","full_name":"Neubergerová, Michaela","first_name":"Michaela"},{"full_name":"Studtrucker, Tanja","last_name":"Studtrucker","first_name":"Tanja"},{"full_name":"Pleskot, Roman","last_name":"Pleskot","first_name":"Roman"},{"full_name":"Dietrich, Petra","last_name":"Dietrich","first_name":"Petra"},{"orcid":"0000-0002-9767-8699","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych","full_name":"Fendrych, Matyas"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","abstract":[{"lang":"eng","text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants."}],"date_created":"2026-01-13T14:07:58Z","status":"public","corr_author":"1","publication":"bioRxiv","related_material":{"record":[{"status":"public","id":"20964","relation":"dissertation_contains"}]}},{"page":"2","project":[{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"},{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"}],"oa_version":"Published Version","intvolume":" 1","file_date_updated":"2026-02-10T09:35:43Z","volume":1,"article_processing_charge":"Yes (in subscription journal)","publisher":"Springer Nature","publication_identifier":{"eissn":["3005-1401"]},"citation":{"ista":"Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2.","apa":"Monzer, A., & Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. Npj Science of Plants. Springer Nature. https://doi.org/10.1038/s44383-025-00002-8","ama":"Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 2025;1(1):2. doi:10.1038/s44383-025-00002-8","chicago":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants. Springer Nature, 2025. https://doi.org/10.1038/s44383-025-00002-8.","short":"A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2.","ieee":"A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” npj Science of Plants, vol. 1, no. 1. Springer Nature, p. 2, 2025.","mla":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:10.1038/s44383-025-00002-8."},"date_published":"2025-07-01T00:00:00Z","_id":"21136","corr_author":"1","quality_controlled":"1","issue":"1","external_id":{"pmid":["40630787"]},"author":[{"first_name":"Aline","full_name":"Monzer, Aline","last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"has_accepted_license":"1","doi":"10.1038/s44383-025-00002-8","title":"Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.","abstract":[{"text":"The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development.","lang":"eng"}],"article_type":"original","publication_status":"published","type":"journal_article","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"day":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","OA_place":"publisher","file":[{"file_size":974106,"date_updated":"2026-02-10T09:35:43Z","access_level":"open_access","checksum":"6c190faacf0e3bef98311dc8a12132d4","creator":"dernst","relation":"main_file","date_created":"2026-02-10T09:35:43Z","file_id":"21208","content_type":"application/pdf","success":1,"file_name":"2025_NPJSciencePlants_Monzer.pdf"}],"acknowledgement":"We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript.","month":"07","oa":1,"date_updated":"2026-02-10T09:39:20Z","language":[{"iso":"eng"}],"pmid":1,"department":[{"_id":"JiFr"},{"_id":"GradSch"}],"publication":"npj Science of Plants","status":"public","date_created":"2026-02-03T13:03:53Z","OA_type":"hybrid","ddc":["580"]},{"type":"book_chapter","publication_status":"published","page":"299-322","day":"02","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","oa_version":"None","date_updated":"2026-02-17T13:28:38Z","month":"05","language":[{"iso":"eng"}],"article_processing_charge":"No","publication_identifier":{"isbn":["9780443157196"]},"date_published":"2025-05-02T00:00:00Z","citation":{"ista":"Qi L, Friml J. 2025.Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Cryptic Enzymes and Moonlighting Proteins. Foundations and Frontiers in Enzymology, , 299–322.","ama":"Qi L, Friml J. Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Irving H, Gehring C, Wong A, eds. Cryptic Enzymes and Moonlighting Proteins. Elsevier; 2025:299-322. doi:10.1016/b978-0-443-15719-6.00015-5","apa":"Qi, L., & Friml, J. (2025). Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In H. Irving, C. Gehring, & A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins (pp. 299–322). Elsevier. https://doi.org/10.1016/b978-0-443-15719-6.00015-5","mla":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving et al., Elsevier, 2025, pp. 299–322, doi:10.1016/b978-0-443-15719-6.00015-5.","ieee":"L. Qi and J. Friml, “Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling,” in Cryptic Enzymes and Moonlighting Proteins, H. Irving, C. Gehring, and A. Wong, Eds. Elsevier, 2025, pp. 299–322.","short":"L. Qi, J. Friml, in:, H. Irving, C. Gehring, A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins, Elsevier, 2025, pp. 299–322.","chicago":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” In Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving, Chris Gehring, and Aloysius Wong, 299–322. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-15719-6.00015-5."},"publisher":"Elsevier","editor":[{"first_name":"Helen","last_name":"Irving","full_name":"Irving, Helen"},{"full_name":"Gehring, Chris","last_name":"Gehring","first_name":"Chris"},{"full_name":"Wong, Aloysius","last_name":"Wong","first_name":"Aloysius"}],"_id":"21255","department":[{"_id":"JiFr"}],"status":"public","quality_controlled":"1","scopus_import":"1","publication":"Cryptic Enzymes and Moonlighting Proteins","date_created":"2026-02-16T15:53:52Z","OA_type":"closed access","alternative_title":["Foundations and Frontiers in Enzymology"],"author":[{"first_name":"Linlin","full_name":"Qi, Linlin","last_name":"Qi"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"doi":"10.1016/b978-0-443-15719-6.00015-5","title":"Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling","abstract":[{"lang":"eng","text":"As an important plant hormone to regulate growth and development, auxin has been investigated for more than a century. It had been clearly demonstrated and well-accepted that the intracellular auxin receptors, TIR1/AFBs, are F-box proteins mediating transcriptional auxin signaling by their E3 ubiquitin ligase activity, which targets and sends for degradation the Aux/IAA transcriptional repressors. The recent discovery of adenylate cyclase (AC) and guanylate cyclase (GC) activities for TIR1/AFBs open entirely new perspectives on how auxin signaling can operate. This chapter traces back the history of how canonical transcriptional auxin signaling was established and introduces the discovery of the TIR1/AFBs-mediated nontranscriptional signaling branch. Finally, the current understanding and open questions of how TIR1/AFBs’ AC and GC activities contribute to the transcriptional and nontranscriptional auxin signaling are discussed, highlighting the possibility that cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) act as second messengers in auxin signal transduction."}]},{"department":[{"_id":"JiFr"}],"pmid":1,"isi":1,"month":"01","date_updated":"2025-05-19T14:02:01Z","oa":1,"language":[{"iso":"eng"}],"acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We thank Yaowei Wang, Yongming Luo, and Nemanja Vukašinović (VIB-UGhent, Belgium) for useful discussions and technical support. L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation. No conflict of interest is declared.","file":[{"access_level":"open_access","file_size":4443183,"date_updated":"2025-04-16T09:02:05Z","checksum":"7b0e4511e43cc0da06730c3edb7c1167","relation":"main_file","creator":"dernst","date_created":"2025-04-16T09:02:05Z","content_type":"application/pdf","file_id":"19575","file_name":"2025_PlantComm_Wei.pdf","success":1}],"OA_place":"publisher","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"13","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"type":"journal_article","article_type":"original","publication_status":"published","article_number":"101181","ddc":["580"],"OA_type":"gold","date_created":"2024-12-04T11:21:16Z","status":"public","publication":"Plant Communications","_id":"18619","date_published":"2025-01-13T00:00:00Z","publication_identifier":{"issn":["2590-3462"]},"citation":{"mla":"Wei, H., et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications, vol. 6, no. 1, 101181, Elsevier, 2025, doi:10.1016/j.xplc.2024.101181.","ieee":"H. Wei et al., “Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1,” Plant Communications, vol. 6, no. 1. Elsevier, 2025.","short":"H. Wei, H. Zhu, W. Ying, H. Janssens, M. Kvasnica, J. Winne, Y. Gao, J. Friml, Q. Ma, S. Tan, X. Liu, E. Russinova, L. Sun, Plant Communications 6 (2025).","chicago":"Wei, H, H Zhu, W Ying, H Janssens, M Kvasnica, JM Winne, Y Gao, et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications. Elsevier, 2025. https://doi.org/10.1016/j.xplc.2024.101181.","ista":"Wei H, Zhu H, Ying W, Janssens H, Kvasnica M, Winne J, Gao Y, Friml J, Ma Q, Tan S, Liu X, Russinova E, Sun L. 2025. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 6(1), 101181.","ama":"Wei H, Zhu H, Ying W, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 2025;6(1). doi:10.1016/j.xplc.2024.101181","apa":"Wei, H., Zhu, H., Ying, W., Janssens, H., Kvasnica, M., Winne, J., … Sun, L. (2025). Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101181"},"publisher":"Elsevier","article_processing_charge":"Yes","volume":6,"file_date_updated":"2025-04-16T09:02:05Z","intvolume":" 6","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signalling initiation, which requires binding of BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter, ABCB19, functions as a BR exporter, and together with its close homologue, ABCB1, positively regulate BR signalling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 was stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states were determined. In the BL-bound structure, BL was bound to the hydrophobic cavity formed by the transmembrane domain, and triggered local conformational changes. Together, our data provide additional insights into the ABC transporter-mediated BR export."}],"title":"Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1","DOAJ_listed":"1","doi":"10.1016/j.xplc.2024.101181","has_accepted_license":"1","author":[{"last_name":"Wei","full_name":"Wei, H","first_name":"H"},{"first_name":"H","full_name":"Zhu, H","last_name":"Zhu"},{"last_name":"Ying","full_name":"Ying, W","first_name":"W"},{"last_name":"Janssens","full_name":"Janssens, H","first_name":"H"},{"last_name":"Kvasnica","full_name":"Kvasnica, M","first_name":"M"},{"full_name":"Winne, JM","last_name":"Winne","first_name":"JM"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Ma","full_name":"Ma, Q","first_name":"Q"},{"first_name":"S","full_name":"Tan, S","last_name":"Tan"},{"first_name":"X","full_name":"Liu, X","last_name":"Liu"},{"full_name":"Russinova, E","last_name":"Russinova","first_name":"E"},{"first_name":"L","full_name":"Sun, L","last_name":"Sun"}],"external_id":{"pmid":["39497419"],"isi":["001416757300001"]},"issue":"1","quality_controlled":"1","scopus_import":"1"},{"file":[{"file_size":53904111,"access_level":"open_access","date_updated":"2025-07-31T07:03:43Z","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","creator":"dernst","relation":"main_file","date_created":"2025-07-31T07:03:43Z","content_type":"application/pdf","file_id":"20092","success":1,"file_name":"2025_PlantCell_Gallei.pdf"}],"OA_place":"publisher","day":"01","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","article_type":"original","publication_status":"published","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"pmid":1,"isi":1,"language":[{"iso":"eng"}],"month":"04","oa":1,"date_updated":"2026-06-10T08:30:19Z","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","PlanS_conform":"1","ddc":["580"],"related_material":{"record":[{"relation":"earlier_version","id":"18689","status":"public"},{"relation":"research_data","id":"18837","status":"public"}]},"OA_type":"hybrid","date_created":"2025-02-05T06:52:06Z","status":"public","publication":"The Plant Cell","article_number":"koaf006","file_date_updated":"2025-07-31T07:03:43Z","oa_version":"Published Version","intvolume":" 37","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"grant_number":"665385","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","grant_number":"ALTF 679-2018","_id":"269B5B22-B435-11E9-9278-68D0E5697425"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"call_identifier":"FWF","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","name":"Molecular Drug Targets"}],"_id":"19003","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"citation":{"ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006","ieee":"M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025.","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University Press, 2025. https://doi.org/10.1093/plcell/koaf006.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:10.1093/plcell/koaf006."},"date_published":"2025-04-01T00:00:00Z","publisher":"Oxford University Press","article_processing_charge":"Yes (via OA deal)","volume":37,"external_id":{"pmid":["39792900"],"isi":["001462763100001"]},"issue":"4","corr_author":"1","scopus_import":"1","quality_controlled":"1","ec_funded":1,"title":"Super-resolution expansion microscopy in plant roots","abstract":[{"lang":"eng","text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. "}],"doi":"10.1093/plcell/koaf006","has_accepted_license":"1","author":[{"full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","orcid":"0000-0003-1286-7368"},{"first_name":"Sven M","last_name":"Truckenbrodt","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M"},{"full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","last_name":"Kreuzinger","first_name":"Caroline"},{"first_name":"Syamala","orcid":"0009-0002-5890-120X","full_name":"Inumella, Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","last_name":"Inumella"},{"full_name":"Vistunou, Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M","first_name":"Christoph M","orcid":"0000-0003-1216-9105"},{"id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","last_name":"Tavakoli","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba","orcid":"0000-0002-7667-6854"},{"last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","full_name":"Agudelo Duenas, Nathalie","first_name":"Nathalie"},{"first_name":"Jakob","orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","last_name":"Vorlaufer"},{"last_name":"Jahr","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","first_name":"Wiebke","orcid":"0000-0003-0201-2315"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","full_name":"Randuch, Marek","first_name":"Marek"},{"full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","orcid":"0000-0002-2739-8843","first_name":"Alexander J"},{"last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","first_name":"Johann G"}]},{"scopus_import":"1","quality_controlled":"1","corr_author":"1","external_id":{"pmid":["39829340"],"isi":["001436802900001"]},"doi":"10.1093/pcp/pcaf008","author":[{"last_name":"Tang","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","full_name":"Tang, Han","first_name":"Han","orcid":"0000-0001-6152-6637"},{"first_name":"L","full_name":"Chen, L","last_name":"Chen"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"ec_funded":1,"title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","abstract":[{"text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species.","lang":"eng"}],"project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}],"oa_version":"None","article_processing_charge":"No","_id":"19420","citation":{"ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. 2025. doi:10.1093/pcp/pcaf008","apa":"Tang, H., Chen, L., & Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcaf008","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology, pcaf008, Oxford University Press, 2025, doi:10.1093/pcp/pcaf008.","short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” Plant and Cell Physiology. Oxford University Press, 2025.","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology. Oxford University Press, 2025. https://doi.org/10.1093/pcp/pcaf008."},"date_published":"2025-03-05T00:00:00Z","publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"publisher":"Oxford University Press","date_created":"2025-03-19T09:44:19Z","OA_type":"closed access","status":"public","publication":"Plant and Cell Physiology","article_number":"pcaf008","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"05","year":"2025","type":"journal_article","publication_status":"published","article_type":"original","date_updated":"2025-09-30T11:05:55Z","language":[{"iso":"eng"}],"month":"03","acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","department":[{"_id":"JiFr"}],"pmid":1,"isi":1},{"external_id":{"pmid":["40044868"],"isi":["001437493900001"]},"quality_controlled":"1","corr_author":"1","abstract":[{"text":"The phytohormone auxin (Aux) is a principal endogenous developmental signal in plants. It mediates transcriptional reprogramming by a well-established canonical signalling mechanism. TIR1/AFB auxin receptors are F-box subunits of an ubiquitin ligase complex; after auxin perception, they associate with Aux/IAA transcriptional repressors and ubiquitinate them for degradation, thus enabling the activation of auxin response factor (ARF) transcription factors1,2,3. Here we revise this paradigm by showing that without TIR1 adenylate cyclase (AC) activity4, auxin-induced degradation of Aux/IAAs is not sufficient to mediate the transcriptional auxin response. Abolishing the TIR1 AC activity does not affect auxin-induced degradation of Aux/IAAs but renders TIR1 non-functional in mediating transcriptional reprogramming and auxin-regulated development, including shoot, root, root hair growth and lateral root formation. Transgenic plants show that local cAMP production in the vicinity of the Aux/IAA–ARF complex by unrelated AC enzymes bypasses the need for auxin perception and is sufficient to induce ARF-mediated transcription. These discoveries revise the canonical model of auxin signalling and establish TIR1/AFB-produced cAMP as a second messenger essential for transcriptional reprograming.","lang":"eng"}],"title":"TIR1-produced cAMP as a second messenger in transcriptional auxin signalling","doi":"10.1038/s41586-025-08669-w","author":[{"first_name":"Huihuang","full_name":"Chen, Huihuang","id":"83c96512-15b2-11ec-abd3-b7eede36184f","last_name":"Chen"},{"orcid":"0000-0001-5187-8401","first_name":"Linlin","last_name":"Qi","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","full_name":"Qi, Linlin"},{"first_name":"Minxia","full_name":"Zou, Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9"},{"first_name":"Mengting","full_name":"Lu, Mengting","last_name":"Lu","id":"a8198a14-1ffe-11ee-8b67-d2bdff9d9178"},{"first_name":"M","last_name":"Kwiatkowski","full_name":"Kwiatkowski, M"},{"first_name":"Yuanrong","last_name":"Pei","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","full_name":"Pei, Yuanrong"},{"last_name":"Jaworski","full_name":"Jaworski, K","first_name":"K"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"has_accepted_license":"1","file_date_updated":"2025-08-05T12:29:35Z","oa_version":"Published Version","intvolume":" 640","project":[{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"page":"1011-1016","_id":"19421","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"date_published":"2025-04-24T00:00:00Z","citation":{"ista":"Chen H, Qi L, Zou M, Lu M, Kwiatkowski M, Pei Y, Jaworski K, Friml J. 2025. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 640, 1011–1016.","apa":"Chen, H., Qi, L., Zou, M., Lu, M., Kwiatkowski, M., Pei, Y., … Friml, J. (2025). TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08669-w","ama":"Chen H, Qi L, Zou M, et al. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 2025;640:1011-1016. doi:10.1038/s41586-025-08669-w","chicago":"Chen, Huihuang, Linlin Qi, Minxia Zou, Mengting Lu, M Kwiatkowski, Yuanrong Pei, K Jaworski, and Jiří Friml. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-08669-w.","short":"H. Chen, L. Qi, M. Zou, M. Lu, M. Kwiatkowski, Y. Pei, K. Jaworski, J. Friml, Nature 640 (2025) 1011–1016.","ieee":"H. Chen et al., “TIR1-produced cAMP as a second messenger in transcriptional auxin signalling,” Nature, vol. 640. Springer Nature, pp. 1011–1016, 2025.","mla":"Chen, Huihuang, et al. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” Nature, vol. 640, Springer Nature, 2025, pp. 1011–16, doi:10.1038/s41586-025-08669-w."},"publisher":"Springer Nature","article_processing_charge":"Yes (via OA deal)","volume":640,"PlanS_conform":"1","related_material":{"record":[{"relation":"dissertation_contains","id":"19478","status":"public"}],"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/updating-the-textbook/"}]},"ddc":["580"],"OA_type":"hybrid","date_created":"2025-03-19T09:44:39Z","status":"public","publication":"Nature","file":[{"date_created":"2025-08-05T12:29:35Z","file_id":"20132","content_type":"application/pdf","success":1,"file_name":"2025_Nature_Chen.pdf","date_updated":"2025-08-05T12:29:35Z","access_level":"open_access","file_size":13549245,"checksum":"f5f18081003e7a1b8e372ecb7da82e7d","creator":"dernst","relation":"main_file"}],"OA_place":"publisher","day":"24","year":"2025","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","article_type":"original","publication_status":"published","department":[{"_id":"JiFr"}],"pmid":1,"isi":1,"language":[{"iso":"eng"}],"date_updated":"2026-04-28T13:42:45Z","month":"04","oa":1,"acknowledgement":"We are grateful to J. Callis and H.-Q. Yang for sharing materials and to M. Estelle and S. Kepinski for inspiring discussions. This research was supported by the Laboratory Support Facility, the Plant Facility and the Imaging and Optics Facility of the Institute of Science and Technology Austria. This project has received funding from the European Research Council (101142681 CYNIPS) and Austrian Science Fund (P 37051-B). L.Q. was supported by the National Natural Science Foundation of China (grant no. 32470327). M.Z. was supported by the Interdisciplinary Project Committee of the Institute of Science and Technology Austria, and Y.P. was supported by an EMBO Postdoctoral Fellowship (ALTF 38-2023). Open access funding provided by Institute of Science and Technology (IST Austria)."},{"external_id":{"isi":["001437953800001"],"pmid":["40044942"]},"scopus_import":"1","quality_controlled":"1","title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","abstract":[{"text":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops.","lang":"eng"}],"author":[{"full_name":"Jia, G","last_name":"Jia","first_name":"G"},{"first_name":"G","full_name":"Chen, G","last_name":"Chen"},{"full_name":"Zhang, Z","last_name":"Zhang","first_name":"Z"},{"first_name":"C","last_name":"Tian","full_name":"Tian, C"},{"full_name":"Wang, Y","last_name":"Wang","first_name":"Y"},{"first_name":"J","full_name":"Luo, J","last_name":"Luo"},{"first_name":"K","last_name":"Zhang","full_name":"Zhang, K"},{"first_name":"X","last_name":"Zhao","full_name":"Zhao, X"},{"last_name":"Zhao","full_name":"Zhao, X","first_name":"X"},{"full_name":"Li, Z","last_name":"Li","first_name":"Z"},{"last_name":"Sun","full_name":"Sun, L","first_name":"L"},{"full_name":"Yang, W","last_name":"Yang","first_name":"W"},{"last_name":"Guo","full_name":"Guo, Y","first_name":"Y"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Gong, Z","last_name":"Gong","first_name":"Z"},{"first_name":"J","full_name":"Zhang, J","last_name":"Zhang"}],"has_accepted_license":"1","doi":"10.1038/s41477-025-01934-w","oa_version":"Submitted Version","intvolume":" 11","file_date_updated":"2025-11-12T07:50:45Z","publisher":"Springer Nature","date_published":"2025-03-05T00:00:00Z","publication_identifier":{"issn":["2055-0278"]},"citation":{"ama":"Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 2025;11. doi:10.1038/s41477-025-01934-w","apa":"Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025). Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01934-w","ista":"Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.","mla":"Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants, vol. 11, 5207, Springer Nature, 2025, doi:10.1038/s41477-025-01934-w.","short":"G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X. Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants 11 (2025).","chicago":"Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01934-w.","ieee":"G. Jia et al., “Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize,” Nature Plants, vol. 11. Springer Nature, 2025."},"_id":"19422","volume":11,"article_processing_charge":"No","ddc":["580"],"publication":"Nature Plants","status":"public","OA_type":"green","date_created":"2025-03-19T09:44:55Z","article_number":"5207","OA_place":"repository","file":[{"creator":"dernst","relation":"main_file","checksum":"caeaf1a8bc3e1435e8c995d1d9df5390","file_size":2714177,"date_updated":"2025-11-12T07:50:45Z","access_level":"open_access","success":1,"file_name":"2025_NaturePlants_Jia_submitted.pdf","content_type":"application/pdf","file_id":"20634","date_created":"2025-11-12T07:50:45Z"}],"publication_status":"published","article_type":"original","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"05","year":"2025","isi":1,"pmid":1,"department":[{"_id":"JiFr"}],"acknowledgement":"We thank X. Yang for providing published inbred lines and helping with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful discussions. The transgenic maize lines were generated by the Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265 and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.), the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.), the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.), and the Central Guidance on Local Science and Technology Development Fund of Shanxi Province (YDZJSX2024D040 to C.T. and J.Z.).","month":"03","language":[{"iso":"eng"}],"date_updated":"2025-11-12T07:52:06Z","oa":1},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2025","day":"01","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","article_type":"original","publication_status":"published","file":[{"date_updated":"2025-04-16T08:03:36Z","access_level":"open_access","file_size":12841729,"checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","relation":"main_file","creator":"dernst","date_created":"2025-04-16T08:03:36Z","content_type":"application/pdf","file_id":"19571","file_name":"2025_NewPhytologist_Kurtovic.pdf","success":1}],"OA_place":"publisher","month":"05","language":[{"iso":"eng"}],"oa":1,"date_updated":"2025-09-30T11:11:18Z","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","department":[{"_id":"JiFr"}],"pmid":1,"isi":1,"date_created":"2025-03-19T09:45:11Z","OA_type":"hybrid","status":"public","publication":"New Phytologist","ddc":["580"],"project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"page":"1066-1083","file_date_updated":"2025-04-16T08:03:36Z","oa_version":"Published Version","intvolume":" 246","article_processing_charge":"Yes (via OA deal)","volume":246,"_id":"19423","citation":{"ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 2025;246(3):1066-1083. doi:10.1111/nph.70019","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. Wiley. https://doi.org/10.1111/nph.70019","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:10.1111/nph.70019.","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist. Wiley, 2025. https://doi.org/10.1111/nph.70019.","ieee":"K. Kurtović et al., “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” New Phytologist, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.","short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083."},"date_published":"2025-05-01T00:00:00Z","publication_identifier":{"issn":["1469-8137"]},"publisher":"Wiley","issue":"3","scopus_import":"1","quality_controlled":"1","external_id":{"isi":["001438711600001"],"pmid":["40047465"]},"doi":"10.1111/nph.70019","author":[{"full_name":"Kurtović, K","last_name":"Kurtović","first_name":"K"},{"first_name":"S","last_name":"Vosolsobě","full_name":"Vosolsobě, S"},{"first_name":"D","full_name":"Nedvěd, D","last_name":"Nedvěd"},{"full_name":"Müller, K","last_name":"Müller","first_name":"K"},{"full_name":"Dobrev, PI","last_name":"Dobrev","first_name":"PI"},{"full_name":"Schmidt, V","last_name":"Schmidt","first_name":"V"},{"full_name":"Piszczek, P","last_name":"Piszczek","first_name":"P"},{"full_name":"Kuhn, A","last_name":"Kuhn","first_name":"A"},{"first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","full_name":"Smoljan, Adrijana"},{"first_name":"TJ","full_name":"Fisher, TJ","last_name":"Fisher"},{"first_name":"D","full_name":"Weijers, D","last_name":"Weijers"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Bowman","full_name":"Bowman, JL","first_name":"JL"},{"last_name":"Petrášek","full_name":"Petrášek, J","first_name":"J"}],"has_accepted_license":"1","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","abstract":[{"text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.","lang":"eng"}]},{"type":"journal_article","article_type":"letter_note","publication_status":"published","year":"2025","day":"11","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"OA_place":"publisher","file":[{"relation":"main_file","creator":"dernst","checksum":"8225c1899bb2f39f9a1707cc0697a052","file_size":7062474,"date_updated":"2025-12-30T07:28:09Z","access_level":"open_access","file_name":"2025_NaturePlants_deRoij.pdf","success":1,"content_type":"application/pdf","file_id":"20882","date_created":"2025-12-30T07:28:09Z"}],"acknowledgement":"We thank S. Woudenberg, S. Valk and J. Rienstra for help and advice, A. Kuhn for comments on the paper and M. Prigge and M. Estelle for helpful discussions. This work was supported by a grant from Netherlands Organization for Scientific Research (NWO; OCENW.M20.031 to J.W.B.), a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2020 contract number to J.H.G.) and a research grant from the Human Frontiers Research Program (HFSP; grant RGP0015/2022 to D.W.).","language":[{"iso":"eng"}],"oa":1,"date_updated":"2025-12-30T07:28:49Z","month":"04","pmid":1,"department":[{"_id":"JiFr"}],"status":"public","publication":"Nature Plants","OA_type":"hybrid","date_created":"2025-04-20T22:01:28Z","ddc":["580"],"page":"717-724","file_date_updated":"2025-12-30T07:28:09Z","intvolume":" 11","oa_version":"Published Version","volume":11,"article_processing_charge":"Yes (in subscription journal)","citation":{"ista":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. 2025. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 11, 717–724.","ama":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 2025;11:717-724. doi:10.1038/s41477-025-01975-1","apa":"De Roij, M., Hernández García, J., Das, S., Borst, J. W., & Weijers, D. (2025). ARF degradation defines a deeply conserved step in auxin response. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01975-1","mla":"De Roij, Martijn, et al. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants, vol. 11, Springer Nature, 2025, pp. 717–24, doi:10.1038/s41477-025-01975-1.","ieee":"M. De Roij, J. Hernández García, S. Das, J. W. Borst, and D. Weijers, “ARF degradation defines a deeply conserved step in auxin response,” Nature Plants, vol. 11. Springer Nature, pp. 717–724, 2025.","short":"M. De Roij, J. Hernández García, S. Das, J.W. Borst, D. Weijers, Nature Plants 11 (2025) 717–724.","chicago":"De Roij, Martijn, Jorge Hernández García, Shubhajit Das, Jan Willem Borst, and Dolf Weijers. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01975-1."},"publication_identifier":{"eissn":["2055-0278"]},"date_published":"2025-04-11T00:00:00Z","publisher":"Springer Nature","_id":"19601","scopus_import":"1","quality_controlled":"1","external_id":{"pmid":["40216983"]},"author":[{"first_name":"Martijn","last_name":"De Roij","full_name":"De Roij, Martijn"},{"first_name":"Jorge","last_name":"Hernández García","full_name":"Hernández García, Jorge"},{"first_name":"Shubhajit","id":"b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be","last_name":"Das","full_name":"Das, Shubhajit"},{"first_name":"Jan Willem","full_name":"Borst, Jan Willem","last_name":"Borst"},{"full_name":"Weijers, Dolf","last_name":"Weijers","first_name":"Dolf"}],"has_accepted_license":"1","doi":"10.1038/s41477-025-01975-1","title":"ARF degradation defines a deeply conserved step in auxin response","abstract":[{"lang":"eng","text":"In land plants, the signalling molecule auxin profoundly controls growth and development, chiefly through a transcriptional response system. The auxin response is mediated by modulating the activity of DNA-binding auxin response factor (ARF) proteins. The concentrations and stoichiometry of the competing A- and B-class ARFs define cells’ capacity for auxin response. In the minimal auxin response system of the liverwort Marchantia polymorpha, both A- and B-ARFs are unstable, but the underlying mechanisms, developmental relevance and evolutionary history of this instability are unknown. Here we identify a minimal motif that is necessary for MpARF2 (B-class) degradation and show that it is critical for development and the auxin response. Through comparative analysis and motif swaps among all ARF classes in extant algae and land plants, we infer that the emergence of ARF instability probably occurred in the ancestor of the A- and B-ARF clades and, therefore, preceded or coincided with the origin of the auxin response system."}]}]