[{"day":"16","month":"05","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","year":"2025","citation":{"apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. https://doi.org/10.1101/2025.01.06.631460","ieee":"I. Kulich et al., “Armadillo repeat only proteins are required for the function of plant CNGC channels,” bioRxiv. .","ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, 10.1101/2025.01.06.631460.","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. doi:10.1101/2025.01.06.631460","mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, doi:10.1101/2025.01.06.631460.","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, n.d. https://doi.org/10.1101/2025.01.06.631460."},"_id":"20982","status":"public","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"oa_version":"Preprint","publication_status":"draft","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20964"}]},"date_created":"2026-01-13T14:07:58Z","article_processing_charge":"No","publication":"bioRxiv","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","author":[{"first_name":"Ivan","full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich"},{"first_name":"Denisa","full_name":"Oulehlová, Denisa","last_name":"Oulehlová"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou","full_name":"Zou, Minxia"},{"first_name":"Edita","last_name":"Lileikyte","full_name":"Lileikyte, Edita"},{"last_name":"Bondar","full_name":"Bondar, Alexey","first_name":"Alexey"},{"full_name":"Kulichová, Katarína","last_name":"Kulichová","first_name":"Katarína"},{"first_name":"Martin","last_name":"Janda","full_name":"Janda, Martin"},{"last_name":"Iakovenko","full_name":"Iakovenko, Oksana","first_name":"Oksana"},{"full_name":"Neubergerová, Michaela","last_name":"Neubergerová","first_name":"Michaela"},{"first_name":"Tanja","full_name":"Studtrucker, Tanja","last_name":"Studtrucker"},{"first_name":"Roman","last_name":"Pleskot","full_name":"Pleskot, Roman"},{"last_name":"Dietrich","full_name":"Dietrich, Petra","first_name":"Petra"},{"orcid":"0000-0002-9767-8699","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych","full_name":"Fendrych, Matyas"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"abstract":[{"text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants.","lang":"eng"}],"corr_author":"1","date_updated":"2026-04-07T11:41:43Z","OA_place":"repository","title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","oa":1,"date_published":"2025-05-16T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.01.06.631460"}],"fulldoi":"https://doi.org/10.1101/2025.01.06.631460","doi":"10.1101/2025.01.06.631460","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"type":"preprint"},{"file":[{"file_id":"21208","success":1,"file_size":974106,"date_created":"2026-02-10T09:35:43Z","file_name":"2025_NPJSciencePlants_Monzer.pdf","relation":"main_file","checksum":"6c190faacf0e3bef98311dc8a12132d4","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2026-02-10T09:35:43Z"}],"intvolume":" 1","external_id":{"pmid":["40630787"]},"issue":"1","year":"2025","citation":{"mla":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:10.1038/s44383-025-00002-8.","short":"A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2.","chicago":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants. Springer Nature, 2025. https://doi.org/10.1038/s44383-025-00002-8.","apa":"Monzer, A., & Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. Npj Science of Plants. Springer Nature. https://doi.org/10.1038/s44383-025-00002-8","ieee":"A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” npj Science of Plants, vol. 1, no. 1. Springer Nature, p. 2, 2025.","ama":"Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 2025;1(1):2. doi:10.1038/s44383-025-00002-8","ista":"Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2."},"acknowledgement":"We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript.","publisher":"Springer Nature","day":"01","oa_version":"Published Version","status":"public","_id":"21136","OA_place":"publisher","article_type":"original","fulldoi":"https://doi.org/10.1038/s44383-025-00002-8","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"ddc":["580"],"date_published":"2025-07-01T00:00:00Z","has_accepted_license":"1","OA_type":"hybrid","file_date_updated":"2026-02-10T09:35:43Z","abstract":[{"text":"The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-02-10T09:39:20Z","quality_controlled":"1","project":[{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"},{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"}],"date_created":"2026-02-03T13:03:53Z","article_processing_charge":"Yes (in subscription journal)","month":"07","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"language":[{"iso":"eng"}],"volume":1,"publication_status":"published","page":"2","doi":"10.1038/s44383-025-00002-8","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["3005-1401"]},"title":"Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.","oa":1,"author":[{"first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","full_name":"Monzer, Aline"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"npj Science of Plants","corr_author":"1"},{"quality_controlled":"1","date_created":"2026-02-16T15:53:52Z","article_processing_charge":"No","citation":{"ista":"Qi L, Friml J. 2025.Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Cryptic Enzymes and Moonlighting Proteins. Foundations and Frontiers in Enzymology, , 299–322.","ama":"Qi L, Friml J. Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Irving H, Gehring C, Wong A, eds. Cryptic Enzymes and Moonlighting Proteins. Elsevier; 2025:299-322. doi:10.1016/b978-0-443-15719-6.00015-5","ieee":"L. Qi and J. Friml, “Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling,” in Cryptic Enzymes and Moonlighting Proteins, H. Irving, C. Gehring, and A. Wong, Eds. Elsevier, 2025, pp. 299–322.","apa":"Qi, L., & Friml, J. (2025). Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In H. Irving, C. Gehring, & A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins (pp. 299–322). Elsevier. https://doi.org/10.1016/b978-0-443-15719-6.00015-5","chicago":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” In Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving, Chris Gehring, and Aloysius Wong, 299–322. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-15719-6.00015-5.","mla":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving et al., Elsevier, 2025, pp. 299–322, doi:10.1016/b978-0-443-15719-6.00015-5.","short":"L. Qi, J. Friml, in:, H. Irving, C. Gehring, A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins, Elsevier, 2025, pp. 299–322."},"year":"2025","publisher":"Elsevier","day":"02","month":"05","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"editor":[{"first_name":"Helen","last_name":"Irving","full_name":"Irving, Helen"},{"first_name":"Chris","last_name":"Gehring","full_name":"Gehring, Chris"},{"first_name":"Aloysius","last_name":"Wong","full_name":"Wong, Aloysius"}],"publication_status":"published","oa_version":"None","_id":"21255","page":"299-322","status":"public","doi":"10.1016/b978-0-443-15719-6.00015-5","fulldoi":"https://doi.org/10.1016/b978-0-443-15719-6.00015-5","type":"book_chapter","publication_identifier":{"isbn":["9780443157196"]},"OA_type":"closed access","title":"Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling","date_published":"2025-05-02T00:00:00Z","abstract":[{"lang":"eng","text":"As an important plant hormone to regulate growth and development, auxin has been investigated for more than a century. It had been clearly demonstrated and well-accepted that the intracellular auxin receptors, TIR1/AFBs, are F-box proteins mediating transcriptional auxin signaling by their E3 ubiquitin ligase activity, which targets and sends for degradation the Aux/IAA transcriptional repressors. The recent discovery of adenylate cyclase (AC) and guanylate cyclase (GC) activities for TIR1/AFBs open entirely new perspectives on how auxin signaling can operate. This chapter traces back the history of how canonical transcriptional auxin signaling was established and introduces the discovery of the TIR1/AFBs-mediated nontranscriptional signaling branch. Finally, the current understanding and open questions of how TIR1/AFBs’ AC and GC activities contribute to the transcriptional and nontranscriptional auxin signaling are discussed, highlighting the possibility that cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) act as second messengers in auxin signal transduction."}],"author":[{"full_name":"Qi, Linlin","last_name":"Qi","first_name":"Linlin"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"publication":"Cryptic Enzymes and Moonlighting Proteins","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","date_updated":"2026-02-17T13:28:38Z","alternative_title":["Foundations and Frontiers in Enzymology"]},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species.","lang":"eng"}],"ec_funded":1,"date_updated":"2025-09-30T11:05:55Z","date_published":"2025-03-05T00:00:00Z","OA_type":"closed access","fulldoi":"https://doi.org/10.1093/pcp/pcaf008","article_type":"original","day":"05","isi":1,"acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","citation":{"ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. 2025. doi:10.1093/pcp/pcaf008","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” Plant and Cell Physiology. Oxford University Press, 2025.","apa":"Tang, H., Chen, L., & Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcaf008","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology. Oxford University Press, 2025. https://doi.org/10.1093/pcp/pcaf008.","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology, pcaf008, Oxford University Press, 2025, doi:10.1093/pcp/pcaf008.","short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025)."},"year":"2025","publisher":"Oxford University Press","status":"public","_id":"19420","oa_version":"None","external_id":{"isi":["001436802900001"],"pmid":["39829340"]},"publication":"Plant and Cell Physiology","author":[{"id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","last_name":"Tang","full_name":"Tang, Han","orcid":"0000-0001-6152-6637","first_name":"Han"},{"full_name":"Chen, L","last_name":"Chen","first_name":"L"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"corr_author":"1","scopus_import":"1","article_number":"pcaf008","pmid":1,"publication_identifier":{"eissn":["1471-9053"],"issn":["0032-0781"]},"title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","doi":"10.1093/pcp/pcaf008","type":"journal_article","month":"03","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"}],"quality_controlled":"1","date_created":"2025-03-19T09:44:19Z","article_processing_charge":"No"},{"file":[{"access_level":"open_access","checksum":"caeaf1a8bc3e1435e8c995d1d9df5390","relation":"main_file","creator":"dernst","date_updated":"2025-11-12T07:50:45Z","content_type":"application/pdf","success":1,"file_id":"20634","file_size":2714177,"date_created":"2025-11-12T07:50:45Z","file_name":"2025_NaturePlants_Jia_submitted.pdf"}],"intvolume":" 11","external_id":{"pmid":["40044942"],"isi":["001437953800001"]},"oa_version":"Submitted Version","status":"public","_id":"19422","year":"2025","citation":{"ieee":"G. Jia et al., “Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize,” Nature Plants, vol. 11. Springer Nature, 2025.","apa":"Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025). Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01934-w","ista":"Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.","ama":"Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 2025;11. doi:10.1038/s41477-025-01934-w","short":"G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X. Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants 11 (2025).","mla":"Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants, vol. 11, 5207, Springer Nature, 2025, doi:10.1038/s41477-025-01934-w.","chicago":"Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01934-w."},"acknowledgement":"We thank X. Yang for providing published inbred lines and helping with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful discussions. The transgenic maize lines were generated by the Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265 and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.), the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.), the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.), and the Central Guidance on Local Science and Technology Development Fund of Shanxi Province (YDZJSX2024D040 to C.T. and J.Z.).","publisher":"Springer Nature","day":"05","isi":1,"article_type":"original","fulldoi":"https://doi.org/10.1038/s41477-025-01934-w","ddc":["580"],"OA_type":"green","has_accepted_license":"1","date_published":"2025-03-05T00:00:00Z","OA_place":"repository","date_updated":"2025-11-12T07:52:06Z","file_date_updated":"2025-11-12T07:50:45Z","abstract":[{"lang":"eng","text":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-03-19T09:44:55Z","article_processing_charge":"No","quality_controlled":"1","volume":11,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","month":"03","doi":"10.1038/s41477-025-01934-w","type":"journal_article","publication_identifier":{"issn":["2055-0278"]},"pmid":1,"title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","oa":1,"article_number":"5207","scopus_import":"1","author":[{"first_name":"G","full_name":"Jia, G","last_name":"Jia"},{"full_name":"Chen, G","last_name":"Chen","first_name":"G"},{"last_name":"Zhang","full_name":"Zhang, Z","first_name":"Z"},{"last_name":"Tian","full_name":"Tian, C","first_name":"C"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"last_name":"Luo","full_name":"Luo, J","first_name":"J"},{"full_name":"Zhang, K","last_name":"Zhang","first_name":"K"},{"first_name":"X","last_name":"Zhao","full_name":"Zhao, X"},{"full_name":"Zhao, X","last_name":"Zhao","first_name":"X"},{"first_name":"Z","last_name":"Li","full_name":"Li, Z"},{"last_name":"Sun","full_name":"Sun, L","first_name":"L"},{"full_name":"Yang, W","last_name":"Yang","first_name":"W"},{"full_name":"Guo, Y","last_name":"Guo","first_name":"Y"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"full_name":"Gong, Z","last_name":"Gong","first_name":"Z"},{"first_name":"J","full_name":"Zhang, J","last_name":"Zhang"}],"publication":"Nature Plants"},{"oa_version":"Published Version","status":"public","_id":"19423","publisher":"Wiley","year":"2025","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","citation":{"ieee":"K. Kurtović et al., “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” New Phytologist, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. Wiley. https://doi.org/10.1111/nph.70019","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 2025;246(3):1066-1083. doi:10.1111/nph.70019","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:10.1111/nph.70019.","short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083.","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist. Wiley, 2025. https://doi.org/10.1111/nph.70019."},"isi":1,"day":"01","intvolume":" 246","file":[{"creator":"dernst","date_updated":"2025-04-16T08:03:36Z","content_type":"application/pdf","access_level":"open_access","checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","relation":"main_file","file_size":12841729,"date_created":"2025-04-16T08:03:36Z","file_name":"2025_NewPhytologist_Kurtovic.pdf","success":1,"file_id":"19571"}],"issue":"3","external_id":{"pmid":["40047465"],"isi":["001438711600001"]},"date_updated":"2025-09-30T11:11:18Z","file_date_updated":"2025-04-16T08:03:36Z","abstract":[{"lang":"eng","text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1111/nph.70019","article_type":"original","OA_type":"hybrid","date_published":"2025-05-01T00:00:00Z","has_accepted_license":"1","ddc":["580"],"OA_place":"publisher","publication_status":"published","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"volume":246,"page":"1066-1083","license":"https://creativecommons.org/licenses/by/4.0/","month":"05","article_processing_charge":"Yes (via OA deal)","date_created":"2025-03-19T09:45:11Z","quality_controlled":"1","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"scopus_import":"1","author":[{"full_name":"Kurtović, K","last_name":"Kurtović","first_name":"K"},{"full_name":"Vosolsobě, S","last_name":"Vosolsobě","first_name":"S"},{"first_name":"D","last_name":"Nedvěd","full_name":"Nedvěd, D"},{"first_name":"K","last_name":"Müller","full_name":"Müller, K"},{"last_name":"Dobrev","full_name":"Dobrev, PI","first_name":"PI"},{"first_name":"V","full_name":"Schmidt, V","last_name":"Schmidt"},{"first_name":"P","last_name":"Piszczek","full_name":"Piszczek, P"},{"full_name":"Kuhn, A","last_name":"Kuhn","first_name":"A"},{"first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana"},{"full_name":"Fisher, TJ","last_name":"Fisher","first_name":"TJ"},{"full_name":"Weijers, D","last_name":"Weijers","first_name":"D"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"full_name":"Bowman, JL","last_name":"Bowman","first_name":"JL"},{"last_name":"Petrášek","full_name":"Petrášek, J","first_name":"J"}],"publication":"New Phytologist","type":"journal_article","doi":"10.1111/nph.70019","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","oa":1,"publication_identifier":{"issn":["1469-8137"]},"pmid":1},{"date_updated":"2025-12-30T07:28:49Z","file_date_updated":"2025-12-30T07:28:09Z","abstract":[{"lang":"eng","text":"In land plants, the signalling molecule auxin profoundly controls growth and development, chiefly through a transcriptional response system. The auxin response is mediated by modulating the activity of DNA-binding auxin response factor (ARF) proteins. The concentrations and stoichiometry of the competing A- and B-class ARFs define cells’ capacity for auxin response. In the minimal auxin response system of the liverwort Marchantia polymorpha, both A- and B-ARFs are unstable, but the underlying mechanisms, developmental relevance and evolutionary history of this instability are unknown. Here we identify a minimal motif that is necessary for MpARF2 (B-class) degradation and show that it is critical for development and the auxin response. Through comparative analysis and motif swaps among all ARF classes in extant algae and land plants, we infer that the emergence of ARF instability probably occurred in the ancestor of the A- and B-ARF clades and, therefore, preceded or coincided with the origin of the auxin response system."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1038/s41477-025-01975-1","article_type":"letter_note","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"has_accepted_license":"1","OA_type":"hybrid","date_published":"2025-04-11T00:00:00Z","OA_place":"publisher","oa_version":"Published Version","_id":"19601","status":"public","year":"2025","citation":{"apa":"De Roij, M., Hernández García, J., Das, S., Borst, J. W., & Weijers, D. (2025). ARF degradation defines a deeply conserved step in auxin response. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01975-1","ieee":"M. De Roij, J. Hernández García, S. Das, J. W. Borst, and D. Weijers, “ARF degradation defines a deeply conserved step in auxin response,” Nature Plants, vol. 11. Springer Nature, pp. 717–724, 2025.","ama":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 2025;11:717-724. doi:10.1038/s41477-025-01975-1","ista":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. 2025. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 11, 717–724.","short":"M. De Roij, J. Hernández García, S. Das, J.W. Borst, D. Weijers, Nature Plants 11 (2025) 717–724.","mla":"De Roij, Martijn, et al. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants, vol. 11, Springer Nature, 2025, pp. 717–24, doi:10.1038/s41477-025-01975-1.","chicago":"De Roij, Martijn, Jorge Hernández García, Shubhajit Das, Jan Willem Borst, and Dolf Weijers. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01975-1."},"acknowledgement":"We thank S. Woudenberg, S. Valk and J. Rienstra for help and advice, A. Kuhn for comments on the paper and M. Prigge and M. Estelle for helpful discussions. This work was supported by a grant from Netherlands Organization for Scientific Research (NWO; OCENW.M20.031 to J.W.B.), a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2020 contract number to J.H.G.) and a research grant from the Human Frontiers Research Program (HFSP; grant RGP0015/2022 to D.W.).","publisher":"Springer Nature","day":"11","file":[{"success":1,"file_id":"20882","file_name":"2025_NaturePlants_deRoij.pdf","date_created":"2025-12-30T07:28:09Z","file_size":7062474,"access_level":"open_access","checksum":"8225c1899bb2f39f9a1707cc0697a052","relation":"main_file","date_updated":"2025-12-30T07:28:09Z","content_type":"application/pdf","creator":"dernst"}],"intvolume":" 11","external_id":{"pmid":["40216983"]},"scopus_import":"1","author":[{"full_name":"De Roij, Martijn","last_name":"De Roij","first_name":"Martijn"},{"full_name":"Hernández García, Jorge","last_name":"Hernández García","first_name":"Jorge"},{"first_name":"Shubhajit","full_name":"Das, Shubhajit","last_name":"Das","id":"b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be"},{"last_name":"Borst","full_name":"Borst, Jan Willem","first_name":"Jan Willem"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"}],"publication":"Nature Plants","doi":"10.1038/s41477-025-01975-1","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["2055-0278"]},"title":"ARF degradation defines a deeply conserved step in auxin response","oa":1,"language":[{"iso":"eng"}],"volume":11,"department":[{"_id":"JiFr"}],"publication_status":"published","page":"717-724","month":"04","date_created":"2025-04-20T22:01:28Z","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1"},{"project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"},{"grant_number":"I03630","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/how-roots-forage-for-water/"}]},"quality_controlled":"1","article_processing_charge":"Yes (in subscription journal)","APC_amount":"5937,40 EUR","date_created":"2025-05-25T22:16:43Z","month":"05","publication_status":"published","language":[{"iso":"eng"}],"volume":122,"department":[{"_id":"JiFr"}],"article_number":"e2427315122","title":"Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism","oa":1,"publication_identifier":{"eissn":["1091-6490"]},"pmid":1,"type":"journal_article","doi":"10.1073/pnas.2427315122","publication":"Proceedings of the National Academy of Sciences","author":[{"full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"first_name":"Zhulatai","full_name":"Bao, Zhulatai","last_name":"Bao"},{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","first_name":"Adrijana"},{"last_name":"Liu","full_name":"Liu, Yifan","first_name":"Yifan"},{"last_name":"Wang","full_name":"Wang, Huihui","first_name":"Huihui"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","scopus_import":"1","issue":"20","external_id":{"isi":["001496347500001"],"pmid":["40372432"]},"intvolume":" 122","file":[{"file_name":"2025_PNAS_Zhang.pdf","date_created":"2025-05-28T08:04:50Z","file_size":8266672,"success":1,"file_id":"19750","date_updated":"2025-05-28T08:04:50Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","checksum":"f70ff35054561b27a463ba279d1795dc","relation":"main_file"}],"isi":1,"day":"20","publisher":"National Academy of Sciences","citation":{"mla":"Zhang, Yuzhou, et al. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” Proceedings of the National Academy of Sciences, vol. 122, no. 20, e2427315122, National Academy of Sciences, 2025, doi:10.1073/pnas.2427315122.","short":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, J. Friml, Proceedings of the National Academy of Sciences 122 (2025).","chicago":"Zhang, Yuzhou, Zhulatai Bao, Adrijana Smoljan, Yifan Liu, Huihui Wang, and Jiří Friml. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2427315122.","apa":"Zhang, Y., Bao, Z., Smoljan, A., Liu, Y., Wang, H., & Friml, J. (2025). Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2427315122","ieee":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, and J. Friml, “Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism,” Proceedings of the National Academy of Sciences, vol. 122, no. 20. National Academy of Sciences, 2025.","ista":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. 2025. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 122(20), e2427315122.","ama":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 2025;122(20). doi:10.1073/pnas.2427315122"},"acknowledgement":"This work was supported by the European Union’s Horizon 2020 research and innovation Programme (European Research Council grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), (Institute of Science and Technology Austria) Fellow program, the Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program (QCYRCXM-2022-237), the Fundamental Research Funds for Northwest A&F University and partly supported by the open funds of the State Key Laboratory of Plant Environmental Resilience (SKLPERKF2416). We also thank the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly Dr. Ningjuan Fan for technical assistance.","year":"2025","_id":"19728","status":"public","oa_version":"Published Version","OA_place":"publisher","has_accepted_license":"1","OA_type":"hybrid","date_published":"2025-05-20T00:00:00Z","ddc":["580"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1073/pnas.2427315122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"file_date_updated":"2025-05-28T08:04:50Z","abstract":[{"lang":"eng","text":"Root system integrates multiple environmental cues, chiefly gravity and soil humidity, to anchor plants in soil and forage for water. While the mechanism of auxin-mediated root gravitropism is comparably well-understood, the root’s capability to grow toward moist soil for water uptake and drought avoidance, termed root hydrotropism, remains largely mysterious. Here, we provide key insights into the mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism, MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation of antagonistically acting gravitropism, which is inhibited under drought conditions. Drought stress interferes with subcellular trafficking and the lateral mobility of PIN auxin transporters, which are polarly localized at the root cell plasma membranes. This leads to defects in PIN2 polarity and gravity-induced polarization of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending. The miz1 mutant is defective in all these regulations, and in support of MIZ1’s action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant. These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism to promote root hydrotropism for efficient water foraging under drought conditions."}],"date_updated":"2026-05-20T08:34:21Z"},{"month":"05","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","date_created":"2025-05-25T22:16:57Z","article_processing_charge":"No","author":[{"last_name":"Vanneste","full_name":"Vanneste, Steffen","first_name":"Steffen"},{"full_name":"Pei, Yuanrong","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","last_name":"Pei","first_name":"Yuanrong"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"Nature Reviews Molecular Cell Biology","scopus_import":"1","corr_author":"1","article_number":"e113018","doi":"10.1038/s41580-025-00851-2","type":"journal_article","pmid":1,"publication_identifier":{"issn":["1471-0072"],"eissn":["1471-0080"]},"title":"Mechanisms of auxin action in plant growth and development","year":"2025","citation":{"apa":"Vanneste, S., Pei, Y., & Friml, J. (2025). Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. Springer Nature. https://doi.org/10.1038/s41580-025-00851-2","ieee":"S. Vanneste, Y. Pei, and J. Friml, “Mechanisms of auxin action in plant growth and development,” Nature Reviews Molecular Cell Biology. Springer Nature, 2025.","ista":"Vanneste S, Pei Y, Friml J. 2025. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology., e113018.","ama":"Vanneste S, Pei Y, Friml J. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. 2025. doi:10.1038/s41580-025-00851-2","short":"S. Vanneste, Y. Pei, J. Friml, Nature Reviews Molecular Cell Biology (2025).","mla":"Vanneste, Steffen, et al. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology, e113018, Springer Nature, 2025, doi:10.1038/s41580-025-00851-2.","chicago":"Vanneste, Steffen, Yuanrong Pei, and Jiří Friml. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology. Springer Nature, 2025. https://doi.org/10.1038/s41580-025-00851-2."},"publisher":"Springer Nature","day":"19","isi":1,"oa_version":"None","status":"public","_id":"19736","external_id":{"isi":["001490500500001"],"pmid":["40389696"]},"abstract":[{"text":"The phytohormone auxin is a major signal coordinating growth and development in plants. The variety of its effects arises from its ability to form local auxin maxima and gradients within tissues, generated through directional cell-to-cell transport and elaborate metabolic control. These auxin distribution patterns instruct cells in a context-dependent manner to undergo predefined developmental transitions. In this Review, we discuss advances in auxin action at the level of homeostasis and signalling. We highlight key insights into the structural basis of PIN-mediated intercellular auxin transport and explore two novel non-transcriptional auxin signalling mechanisms: one involving intracellular Ca2+ transients and another involving cell-surface auxin perception that mediates global, ultrafast phosphorylation. Furthermore, we examine emerging evidence indicating the involvement of cyclic adenosine monophosphate as a second messenger in the transcriptional auxin response. Together, these recent developments in auxin research have profoundly deepened our understanding of the complex and diverse activities of auxin in plant growth and development.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-30T12:41:30Z","article_type":"review","fulldoi":"https://doi.org/10.1038/s41580-025-00851-2","date_published":"2025-05-19T00:00:00Z","OA_type":"closed access"},{"date_updated":"2025-09-30T14:04:16Z","abstract":[{"lang":"eng","text":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which has not been well described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference with clathrin-mediated endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-trans-Golgi network (TGN)/early endosome (EE) trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during vacuole configuration, most probably carrying the material that allows the establishment of the vacuole in elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional players must be defined."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","fulldoi":"https://doi.org/10.1093/jxb/eraf084","OA_type":"closed access","date_published":"2025-07-02T00:00:00Z","oa_version":"None","status":"public","_id":"20031","citation":{"apa":"Osorio-Navarro, C., Neira-Valenzuela, G., Sierra, P., Adamowski, M., Toledo, J., & Norambuena, L. (2025). The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/eraf084","ieee":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, and L. Norambuena, “The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana,” Journal of Experimental Botany, vol. 76, no. 10. Oxford University Press, pp. 2700–2714, 2025.","ista":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. 2025. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 76(10), 2700–2714.","ama":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 2025;76(10):2700-2714. doi:10.1093/jxb/eraf084","mla":"Osorio-Navarro, Claudio, et al. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” Journal of Experimental Botany, vol. 76, no. 10, Oxford University Press, 2025, pp. 2700–14, doi:10.1093/jxb/eraf084.","short":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, L. Norambuena, Journal of Experimental Botany 76 (2025) 2700–2714.","chicago":"Osorio-Navarro, Claudio, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, and Lorena Norambuena. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” Journal of Experimental Botany. Oxford University Press, 2025. https://doi.org/10.1093/jxb/eraf084."},"year":"2025","acknowledgement":"This research was supported by FONDECYT grants 1170950 and 1211311 and by ANID PhD fellowship 2020-21201663 to PhD student CO-N. The microscopes used in this work were funded by grants FONDEQUIP #EQM 140019 and #EQM12-0003 at the Advanced Microscopy Unit of the Biology Department, Faculty of Science, University of Chile.\r\nWe thank Jiri Friml for donating the XVE»AUXILIN-LIKE2 (AX2) line to support our research. We wish to acknowledge the active and helpful discussion of all the members of the LNM team and the Plant Molecular Biology Centre at Universidad de Chile.","publisher":"Oxford University Press","day":"02","isi":1,"intvolume":" 76","external_id":{"isi":["001482869200001"],"pmid":["40056424"]},"issue":"10","scopus_import":"1","author":[{"first_name":"Claudio","last_name":"Osorio-Navarro","full_name":"Osorio-Navarro, Claudio"},{"first_name":"Gabriel","last_name":"Neira-Valenzuela","full_name":"Neira-Valenzuela, Gabriel"},{"full_name":"Sierra, Paula","last_name":"Sierra","first_name":"Paula"},{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski","full_name":"Adamowski, Maciek","orcid":"0000-0001-6463-5257","first_name":"Maciek"},{"first_name":"Jorge","last_name":"Toledo","full_name":"Toledo, Jorge"},{"full_name":"Norambuena, Lorena","last_name":"Norambuena","first_name":"Lorena"}],"publication":"Journal of Experimental Botany","doi":"10.1093/jxb/eraf084","type":"journal_article","pmid":1,"publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"volume":76,"publication_status":"published","page":"2700-2714","month":"07","date_created":"2025-07-20T22:02:01Z","article_processing_charge":"No","quality_controlled":"1"},{"acknowledgement":"This research was funded by Biological Breeding-National Science and Technology Major Project (2023ZD0407201), China Postdoctoral Science Foundation (2024M763575), China Agricultural University Fund (2025RC042), Chinese Universities Scientific Fund (2024RC031), and Austrian Science Fund (FWF; I 6123-B).","citation":{"ieee":"F. Sheng et al., “Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism,” Proceedings of the National Academy of Sciences, vol. 122, no. 39. National Academy of Sciences, p. e2512274122, 2025.","apa":"Sheng, F., Gao, Y., Wang, Y., Li, Y., Zhang, J., Zhang, Z., … Zhang, J. (2025). Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2512274122","ama":"Sheng F, Gao Y, Wang Y, et al. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 2025;122(39):e2512274122. doi:10.1073/pnas.2512274122","ista":"Sheng F, Gao Y, Wang Y, Li Y, Zhang J, Zhang Z, Qin X, Zhang S, Song W, Li J, Guo Y, Friml J, Gong Z, Zhang Q, Zhang J. 2025. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 122(39), e2512274122.","short":"F. Sheng, Y. Gao, Y. Wang, Y. Li, J. Zhang, Z. Zhang, X. Qin, S. Zhang, W. Song, J. Li, Y. Guo, J. Friml, Z. Gong, Q. Zhang, J. Zhang, Proceedings of the National Academy of Sciences 122 (2025) e2512274122.","mla":"Sheng, F., et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences, vol. 122, no. 39, National Academy of Sciences, 2025, p. e2512274122, doi:10.1073/pnas.2512274122.","chicago":"Sheng, F, Y Gao, Y Wang, Y Li, JA Zhang, Z Zhang, X Qin, et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2512274122."},"year":"2025","publisher":"National Academy of Sciences","day":"23","isi":1,"oa_version":"Published Version","status":"public","_id":"20635","file":[{"success":1,"file_id":"20681","file_size":2667764,"date_created":"2025-11-24T13:48:09Z","file_name":"2025_PNAS_Sheng.pdf","access_level":"open_access","checksum":"38b723a909bf321d7ee537c9d064aa25","relation":"main_file","creator":"dernst","content_type":"application/pdf","date_updated":"2025-11-24T13:48:09Z"}],"intvolume":" 122","external_id":{"pmid":["40986351"],"isi":["001589177800001"]},"issue":"39","file_date_updated":"2025-11-24T13:48:09Z","abstract":[{"lang":"eng","text":"Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s-mediated phosphorylation modulates both the polar localization and transport activity of PIN2. Importantly, SnRK2s function antagonistically to the AGCVIII kinase PID, which phosphorylates PIN2 at a distinct site (S258), establishing a regulatory balance essential for adaptive root growth. Structural modeling and phosphorylation assays further suggest that SnRK2s-mediated phosphorylation at S259 sterically hinders access of PID to S258, providing a mechanistic basis for their antagonistic relationship. These findings uncover a novel regulatory mechanism, by which plants fine-tune root developmental programs to adapt to environmental stimuli, highlighting the evolutionary significance of multilayered kinase-mediated regulation in plant adaptation."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-02-16T12:32:51Z","OA_place":"publisher","article_type":"original","fulldoi":"https://doi.org/10.1073/pnas.2512274122","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"ddc":["580"],"date_published":"2025-09-23T00:00:00Z","OA_type":"hybrid","has_accepted_license":"1","PlanS_conform":"1","month":"09","volume":122,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","page":"e2512274122","quality_controlled":"1","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"date_created":"2025-11-12T10:03:20Z","article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"F","full_name":"Sheng, F","last_name":"Sheng"},{"first_name":"Y","last_name":"Gao","full_name":"Gao, Y"},{"full_name":"Wang, Y","last_name":"Wang","first_name":"Y"},{"last_name":"Li","full_name":"Li, Y","first_name":"Y"},{"first_name":"JA","full_name":"Zhang, JA","last_name":"Zhang"},{"first_name":"Z","full_name":"Zhang, Z","last_name":"Zhang"},{"last_name":"Qin","full_name":"Qin, X","first_name":"X"},{"last_name":"Zhang","full_name":"Zhang, S","first_name":"S"},{"last_name":"Song","full_name":"Song, W","first_name":"W"},{"full_name":"Li, J","last_name":"Li","first_name":"J"},{"first_name":"Y","full_name":"Guo, Y","last_name":"Guo"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"first_name":"Z","full_name":"Gong, Z","last_name":"Gong"},{"first_name":"Q","full_name":"Zhang, Q","last_name":"Zhang"},{"last_name":"Zhang","full_name":"Zhang, J","first_name":"J"}],"publication":"Proceedings of the National Academy of Sciences","scopus_import":"1","doi":"10.1073/pnas.2512274122","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"oa":1,"title":"Antagonistic SnRK2 and PID kinases' action on auxin transport-mediated root gravitropism"},{"language":[{"iso":"eng"}],"volume":122,"department":[{"_id":"JiFr"}],"publication_status":"published","page":"e2506400122","month":"11","date_created":"2025-11-23T23:01:38Z","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","scopus_import":"1","author":[{"first_name":"Suruchi","full_name":"Roychoudhry, Suruchi","last_name":"Roychoudhry"},{"first_name":"Katelyn","full_name":"Sageman-Furnas, Katelyn","last_name":"Sageman-Furnas"},{"last_name":"Taylor","full_name":"Taylor, Harry J.","first_name":"Harry J."},{"last_name":"Showpnil","full_name":"Showpnil, Iftekhar","first_name":"Iftekhar"},{"full_name":"Wolverton, Chris","last_name":"Wolverton","first_name":"Chris"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"full_name":"Bianco, Marta Del","last_name":"Bianco","first_name":"Marta Del"},{"first_name":"Stefan","last_name":"Kepinski","full_name":"Kepinski, Stefan"}],"publication":"Proceedings of the National Academy of Sciences","doi":"10.1073/pnas.2506400122","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["1091-6490"]},"title":"Angle dependence as a unifying feature of root graviresponse modules","oa":1,"oa_version":"Published Version","status":"public","_id":"20663","acknowledgement":"This study was funded by the BBSRC (grant no. BB/N010124/1) and Leverhulme Foundation (grant no. RPG-2018-137) to M.D.B. and S.K., a Fully Funded International Research Scholarship awarded to K.S.-F., and by NASA (grant no. 80NSSC21K0585) to C.W.","year":"2025","citation":{"ista":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, Showpnil I, Wolverton C, Friml J, Bianco MD, Kepinski S. 2025. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 122(46), e2506400122.","ama":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, et al. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 2025;122(46):e2506400122. doi:10.1073/pnas.2506400122","apa":"Roychoudhry, S., Sageman-Furnas, K., Taylor, H. J., Showpnil, I., Wolverton, C., Friml, J., … Kepinski, S. (2025). Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2506400122","ieee":"S. Roychoudhry et al., “Angle dependence as a unifying feature of root graviresponse modules,” Proceedings of the National Academy of Sciences, vol. 122, no. 46. National Academy of Sciences, p. e2506400122, 2025.","chicago":"Roychoudhry, Suruchi, Katelyn Sageman-Furnas, Harry J. Taylor, Iftekhar Showpnil, Chris Wolverton, Jiří Friml, Marta Del Bianco, and Stefan Kepinski. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2506400122.","short":"S. Roychoudhry, K. Sageman-Furnas, H.J. Taylor, I. Showpnil, C. Wolverton, J. Friml, M.D. Bianco, S. Kepinski, Proceedings of the National Academy of Sciences 122 (2025) e2506400122.","mla":"Roychoudhry, Suruchi, et al. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences, vol. 122, no. 46, National Academy of Sciences, 2025, p. e2506400122, doi:10.1073/pnas.2506400122."},"publisher":"National Academy of Sciences","day":"18","file":[{"file_id":"20676","success":1,"file_name":"2025_PNAS_Roychoudhry.pdf","file_size":1394055,"date_created":"2025-11-24T09:48:44Z","relation":"main_file","access_level":"open_access","checksum":"5e1c37dddc5db8fbd0128db4a54c4f6b","content_type":"application/pdf","date_updated":"2025-11-24T09:48:44Z","creator":"dernst"}],"intvolume":" 122","external_id":{"pmid":["41218119"]},"issue":"46","date_updated":"2026-02-16T12:31:31Z","abstract":[{"lang":"eng","text":"Gravitropism, the patterning of postembryonic growth in relation to the gravity vector, allows plants to optimize the use of limited and nonhomogenous resources in their immediate environment. Since the current model of root gravitropism has not been able to integrate all aspects of the response (perception, response, and behavior), research on gravitropism has been dominated by different theories attempting to conceptualize each aspect individually. In this work, we sought to reevaluate all the main components of the root graviresponse through the lens of angle dependence. We show angle dependence in Cholodny–Went-based auxin asymmetry and growth response, which we tracked back to angle-dependent variation in PIN asymmetry and statolith sedimentation in the columella. Thanks to this approach, we were able to suggest distinct roles for PINs and columella cell tiers, and a potential function for auxin vertical flux through the columella. Our findings provide a unifying framework to further explore the mechanisms that regulate angle-dependent gravitropic response, with major implications of time-dependent features of root graviresponse."}],"file_date_updated":"2025-11-24T09:48:44Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1073/pnas.2506400122","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"date_published":"2025-11-18T00:00:00Z","PlanS_conform":"1","has_accepted_license":"1","OA_type":"hybrid","OA_place":"publisher"},{"article_processing_charge":"No","date_created":"2025-03-12T14:28:53Z","related_material":{"record":[{"status":"public","id":"19395","relation":"dissertation_contains"}]},"publication_status":"draft","department":[{"_id":"GradSch"},{"_id":"JiFr"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"month":"03","type":"preprint","doi":"10.1101/2025.02.28.640727","main_file_link":[{"url":"https://doi.org/10.1101/2025.02.28.640727","open_access":"1"}],"oa":1,"title":"TMK interacting network of receptor like kinases for auxin canalization and beyond","corr_author":"1","author":[{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","full_name":"Monzer, Aline","first_name":"Aline"},{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237","first_name":"Lesia"},{"last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","first_name":"Michelle C"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou","full_name":"Zou, Minxia"},{"first_name":"Michael","full_name":"Smejkal, Michael","last_name":"Smejkal","id":"79a5a1be-04a3-11f0-ba18-a1730e0b58e9"},{"first_name":"Ema","last_name":"Cervenova","id":"9f185b95-04a3-11f0-8245-f5e32eeb470f","full_name":"Cervenova, Ema"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"publication":"bioRxiv","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"oa_version":"Published Version","_id":"19398","status":"public","acknowledgement":"We deeply appreciate M. Wrzaczek’s constructive input and insightful discussions, which significantly enriched this work. We thank L. Fiedler for helping with the heat map and for the discussions. We also thank the facilities at ISTA, the imaging and optics (IOF) and Lab Support (LSF) facilities for their service and assistance.","year":"2025","citation":{"ieee":"A. Monzer et al., “TMK interacting network of receptor like kinases for auxin canalization and beyond,” bioRxiv. .","apa":"Monzer, A., Mazur, E., Rodriguez Solovey, L., Gallei, M. C., Zou, M., Smejkal, M., … Friml, J. (n.d.). TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv. https://doi.org/10.1101/2025.02.28.640727","ama":"Monzer A, Mazur E, Rodriguez Solovey L, et al. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv. doi:10.1101/2025.02.28.640727","ista":"Monzer A, Mazur E, Rodriguez Solovey L, Gallei MC, Zou M, Smejkal M, Cervenova E, Friml J. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv, 10.1101/2025.02.28.640727.","short":"A. Monzer, E. Mazur, L. Rodriguez Solovey, M.C. Gallei, M. Zou, M. Smejkal, E. Cervenova, J. Friml, BioRxiv (n.d.).","mla":"Monzer, Aline, et al. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” BioRxiv, doi:10.1101/2025.02.28.640727.","chicago":"Monzer, Aline, Ewa Mazur, Lesia Rodriguez Solovey, Michelle C Gallei, Minxia Zou, Michael Smejkal, Ema Cervenova, and Jiří Friml. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” BioRxiv, n.d. https://doi.org/10.1101/2025.02.28.640727."},"das_tickbox":"1","day":"02","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"fulldoi":"https://doi.org/10.1101/2025.02.28.640727","OA_type":"green","date_published":"2025-03-02T00:00:00Z","has_accepted_license":"1","ddc":["580"],"OA_place":"repository","date_updated":"2026-08-14T09:33:45Z","abstract":[{"lang":"eng","text":"Receptor-like kinases (RLKs), particularly the Transmembrane Kinase (TMK) family, play essential roles in signaling and development, with TMKs being key components of auxin perception and downstream phosphorylation events. While TMKs’ involvement in auxin canalization, a process essential for vasculature formation and regeneration, has been established, nonetheless, the additional signaling and regulatory partners remain poorly understood. In this study, we identify and characterize seven leucine-rich repeat RLKs (TINT1–TINT7) as novel interactors of TMK1, revealing their diverse evolutionary, structural, and functional characteristics. Our results show that TINTs interact with TMK1 and highlight their roles in regulating various developmental processes. Majority of TINTs contributes, together with TMK1, to auxin canalization, with TINT5 linking TMK1 to other canalization component CAMEL. Beyond canalization, we also establish the role of TINT-TMK1 interactions in processes such as stomatal movement and the hypocotyl’s gravitropic response. These findings suggest that TINTs, through their interaction with TMK1, are integral components of various signaling networks, contributing to both auxin canalization and broader plant development."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"month":"03","language":[{"iso":"eng"}],"department":[{"_id":"GradSch"},{"_id":"JiFr"}],"publication_status":"published","page":"160","related_material":{"record":[{"relation":"part_of_dissertation","id":"12291","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"19399"},{"status":"public","relation":"part_of_dissertation","id":"19398"},{"id":"14826","relation":"part_of_dissertation","status":"public"}]},"degree_awarded":"PhD","date_created":"2025-03-12T14:25:42Z","article_processing_charge":"No","author":[{"last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","first_name":"Aline"}],"corr_author":"1","doi_confirm":"1","doi":"10.15479/AT-ISTA-19395","type":"dissertation","publication_identifier":{"eisbn":["978-3-99078-054-1"],"eissn":["2663-337X"]},"title":"Cell-surface auxin signaling: Linking molecular pathways to plant development","oa":1,"citation":{"short":"A. Monzer, Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development, Institute of Science and Technology Austria, 2025.","mla":"Monzer, Aline. Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19395.","chicago":"Monzer, Aline. “Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19395.","ieee":"A. Monzer, “Cell-surface auxin signaling: Linking molecular pathways to plant development,” Institute of Science and Technology Austria, 2025.","apa":"Monzer, A. (2025). Cell-surface auxin signaling: Linking molecular pathways to plant development. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19395","ama":"Monzer A. Cell-surface auxin signaling: Linking molecular pathways to plant development. 2025. doi:10.15479/AT-ISTA-19395","ista":"Monzer A. 2025. Cell-surface auxin signaling: Linking molecular pathways to plant development. Institute of Science and Technology Austria."},"acknowledgement":"I would like to acknowledge the facilities at ISTA, particularly LSF, IOF, and, of course, the plant facility, for providing the necessary resources for my research.","year":"2025","publisher":"Institute of Science and Technology Austria","day":"13","supervisor":[{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"oa_version":"Published Version","_id":"19395","status":"public","file":[{"date_updated":"2025-03-12T14:14:49Z","content_type":"application/pdf","creator":"amonzer","relation":"main_file","access_level":"open_access","checksum":"9a3dd03bb4ec6b9907a325c3c4e8a1d7","file_name":"Final Thesis Aline Monzer.pdf","date_created":"2025-03-12T14:14:49Z","file_size":13119670,"file_id":"19396","success":1},{"date_updated":"2025-04-01T07:55:27Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"amonzer","access_level":"closed","checksum":"a353ce1ee2eabce37bca35499e76dbf1","relation":"source_file","file_name":"Thesis Aline.docx","date_created":"2025-03-12T14:15:19Z","file_size":13774837,"file_id":"19397"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"file_date_updated":"2025-04-01T07:55:27Z","abstract":[{"text":"Plant growth and development rely significantly on phytohormones, with auxin serving as a master regulator, orchestrating processes from embryogenesis to organogenesis, vascular patterning, and environmental adaptation. Since its conceptual proposition by Charles Darwin in 1880 as an endogenous chemical signal influencing phototropism in grass, auxin has captivated scientists seeking to understand how such a small molecule exerts a profound influence on plant development.\r\nOne particularly fascinating aspect of auxin function is its ability to self-organize its transport. Through a feedback mechanism between auxin perception and directional transport—primarily mediated by PIN auxin transporters—auxin establishes narrow transport channels. This phenomenon, known as auxin canalization, is fundamental to vascular formation, regeneration, and other key developmental processes. Despite advances in our understanding, driven by experimental studies and computational models, auxin canalization remains an enigma, with many unanswered questions.\r\nLike other hormones, auxin functions through intricate signaling pathways. It operates through at least two distinct signaling mechanisms: the well-characterized canonical pathway and the less understood non-canonical pathway. While significant progress has been made in elucidating the canonical pathway, the non-canonical mechanisms remain less defined and require further investigation.\r\nIn this study, we revisit the non-canonical auxin signaling pathway mediated by the cell-surface complex Auxin Binding Protein 1-Transmembrane Kinase 1 (ABP1-TMK1), with a particular focus on its downstream phosphorylation events. We reveal that this auxin-mediated phosphorylation is conserved across the green lineage, underscoring its fundamental role in plant development. We explore key phosphorylation targets, particularly PIN2, which is essential for root gravitropism. To further understand TMK1’s role in diverse developmental processes, we identified and investigated its interactors as potential co-receptors or regulatory components within its signaling network.\r\nGiven the previously established role of ABP1-TMK1 in auxin canalization, we sought to further investigate this process and identified several TMK1 interactors also involved in this intricate mechanism.\r\nThese findings provide new insights into the complex regulation of auxin canalization, highlighting a broader and more interconnected signaling framework than previously understood.","lang":"eng"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_updated":"2026-10-15T08:06:16Z","alternative_title":["ISTA Thesis"],"OA_place":"publisher","fulldoi":"https://doi.org/10.15479/AT-ISTA-19395","ddc":["580"],"has_accepted_license":"1","date_published":"2025-03-13T00:00:00Z"},{"file":[{"access_level":"open_access","checksum":"ee03deee47a084b0295251dc49470ad4","relation":"main_file","creator":"dernst","date_updated":"2025-07-22T08:52:17Z","content_type":"application/pdf","success":1,"file_id":"20067","file_size":37708120,"date_created":"2025-07-22T08:52:17Z","file_name":"2025_CellReports_Guan.pdf"}],"intvolume":" 44","external_id":{"isi":["001533244800001"],"pmid":["40668679"]},"issue":"7","oa_version":"Published Version","_id":"20029","status":"public","acknowledgement":"The study was supported by National Natural Science Foundation of China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were run on the Siyuan-1 cluster supported by the Center for High-Performance Computing at Shanghai Jiao Tong University.","year":"2025","citation":{"ama":"Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 2025;44(7). doi:10.1016/j.celrep.2025.116024","ista":"Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 44(7), 116024.","ieee":"B. Guan et al., “Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD,” Cell Reports, vol. 44, no. 7. Cell Press, 2025.","apa":"Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue, H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2025.116024","chicago":"Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports. Cell Press, 2025. https://doi.org/10.1016/j.celrep.2025.116024.","short":"B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W. Xue, Cell Reports 44 (2025).","mla":"Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports, vol. 44, no. 7, 116024, Cell Press, 2025, doi:10.1016/j.celrep.2025.116024."},"publisher":"Cell Press","day":"22","isi":1,"article_type":"original","fulldoi":"https://doi.org/10.1016/j.celrep.2025.116024","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"ddc":["580"],"has_accepted_license":"1","OA_type":"hybrid","date_published":"2025-07-22T00:00:00Z","OA_place":"publisher","date_updated":"2026-10-20T08:22:53Z","file_date_updated":"2025-07-22T08:52:17Z","abstract":[{"lang":"eng","text":"Vacuolar acidification is crucial for the homeostasis of intracellular pH and the recycling of proteins and nutrients in cells, thereby playing important roles in various physiological processes related to vacuolar function. The key factors regulating vacuolar acidification and underlying mechanisms remain unclear. Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency. The pldζ2 mutant massively accumulates autophagic structures while exhibiting premature leaf senescence under nutrient starvation. Impaired autophagic flux, lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2 directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote vacuolar acidification and autophagy under phosphorus starvation. These findings reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide clues in elucidating the regulatory mechanism of vacuolar acidification."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-07-20T22:02:01Z","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","volume":44,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"publication_status":"published","license":"https://creativecommons.org/licenses/by-nc/4.0/","month":"07","doi":"10.1016/j.celrep.2025.116024","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"title":"Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD","oa":1,"article_number":"116024","scopus_import":"1","author":[{"full_name":"Guan, Bin","last_name":"Guan","id":"56aad729-cca2-11ed-a45a-9b4138991a48","first_name":"Bin"},{"last_name":"Xie","full_name":"Xie, Ke Xuan","first_name":"Ke Xuan"},{"first_name":"Xin Qiao","last_name":"Du","full_name":"Du, Xin Qiao"},{"first_name":"Yu Xuan","last_name":"Bai","full_name":"Bai, Yu Xuan"},{"first_name":"Peng Chao","last_name":"Hao","full_name":"Hao, Peng Chao"},{"last_name":"Lin","full_name":"Lin, Wen Hui","first_name":"Wen Hui"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Hong Wei","last_name":"Xue","full_name":"Xue, Hong Wei"}],"publication":"Cell Reports"},{"article_processing_charge":"Yes","date_created":"2025-08-04T13:39:11Z","quality_controlled":"1","publication_status":"published","volume":44,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"month":"07","title":"Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis","oa":1,"publication_identifier":{"eissn":["2211-1247"]},"pmid":1,"type":"journal_article","doi":"10.1016/j.celrep.2025.116056","article_number":"116056","scopus_import":"1","publication":"Cell Reports","author":[{"first_name":"Faqing","full_name":"Xu, Faqing","last_name":"Xu"},{"first_name":"Yongqiang","last_name":"Yu","full_name":"Yu, Yongqiang"},{"first_name":"Bin","full_name":"Guan, Bin","last_name":"Guan","id":"56aad729-cca2-11ed-a45a-9b4138991a48"},{"full_name":"Xu, Tongda","last_name":"Xu","first_name":"Tongda"},{"full_name":"Xu, Zhihong","last_name":"Xu","first_name":"Zhihong"},{"first_name":"Hongwei","last_name":"Xue","full_name":"Xue, Hongwei"}],"issue":"8","external_id":{"isi":["001542038500001"],"pmid":["40714631"]},"intvolume":" 44","file":[{"success":1,"file_id":"20120","date_created":"2025-08-05T06:15:09Z","file_size":24178018,"file_name":"2025_CellReports_Xu.pdf","checksum":"3c43e040a4a7a65ec67ae1d2bb81261a","access_level":"open_access","relation":"main_file","creator":"dernst","date_updated":"2025-08-05T06:15:09Z","content_type":"application/pdf"}],"status":"public","_id":"20116","oa_version":"Published Version","isi":1,"day":"24","publisher":"Cell Press","acknowledgement":"The study was supported by the National Natural Science Foundation of China (NSFC; 32230011, 91954206, and 31721001). We thank Dr. Deli Lin (Shanghai Jiao Tong University) for kind help with the laser confocal microscope observation and the Arabidopsis Biological Resource Center (ABRC) for providing T-DNA insertional mutants.","year":"2025","citation":{"chicago":"Xu, Faqing, Yongqiang Yu, Bin Guan, Tongda Xu, Zhihong Xu, and Hongwei Xue. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” Cell Reports. Cell Press, 2025. https://doi.org/10.1016/j.celrep.2025.116056.","short":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, H. Xue, Cell Reports 44 (2025).","mla":"Xu, Faqing, et al. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” Cell Reports, vol. 44, no. 8, 116056, Cell Press, 2025, doi:10.1016/j.celrep.2025.116056.","ista":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. 2025. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. 44(8), 116056.","ama":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. 2025;44(8). doi:10.1016/j.celrep.2025.116056","apa":"Xu, F., Yu, Y., Guan, B., Xu, T., Xu, Z., & Xue, H. (2025). Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2025.116056","ieee":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, and H. Xue, “Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis,” Cell Reports, vol. 44, no. 8. Cell Press, 2025."},"date_published":"2025-07-24T00:00:00Z","has_accepted_license":"1","OA_type":"gold","ddc":["580"],"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.celrep.2025.116056","DOAJ_listed":"1","OA_place":"publisher","date_updated":"2026-10-20T08:23:15Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Auxin regulates various aspects of plant growth and development by modulating the transcription of target genes through the degradation of auxin/indole-3-acetic acid (Aux/IAA) repressors via the 26S proteasome. Proteasome regulator 1 (PTRE1), a positive regulator of proteasome activity, has been implicated in auxin-mediated proteasome suppression; however, the mechanism by which auxin modulates PTRE1 function remains unclear. Here, we demonstrate that auxin promotes the interaction between germin-like protein 1 (GLP1) and PTRE1, facilitating PTRE1 retention at the plasma membrane. The relocation of PTRE1 results in reduced nuclear 26S proteasome activity, and thus the attenuated Aux/IAA degradation and altered Aux/IAA homeostasis, ultimately resulting in suppressed auxin-mediated transcriptional regulation. Our findings uncover a previously uncharacterized regulatory axis in auxin signaling that controls Aux/IAA protein stability, functioning alongside the TIR1- and TRANSMEMBRANE KINASE 1 (TMK1)-mediated pathways, and highlight the coordination of auxin signaling from the cell surface to the nucleus via auxin-induced PTRE1 relocation, which fine-tunes Aux/IAA protein homeostasis and auxin responses."}],"file_date_updated":"2025-08-05T06:15:09Z"},{"article_number":"101181","type":"journal_article","doi":"10.1016/j.xplc.2024.101181","oa":1,"title":"Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1","pmid":1,"publication_identifier":{"issn":["2590-3462"]},"author":[{"first_name":"H","full_name":"Wei, H","last_name":"Wei"},{"full_name":"Zhu, H","last_name":"Zhu","first_name":"H"},{"last_name":"Ying","full_name":"Ying, W","first_name":"W"},{"first_name":"H","full_name":"Janssens, H","last_name":"Janssens"},{"last_name":"Kvasnica","full_name":"Kvasnica, M","first_name":"M"},{"full_name":"Winne, JM","last_name":"Winne","first_name":"JM"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"first_name":"Q","last_name":"Ma","full_name":"Ma, Q"},{"full_name":"Tan, S","last_name":"Tan","first_name":"S"},{"first_name":"X","full_name":"Liu, X","last_name":"Liu"},{"first_name":"E","full_name":"Russinova, E","last_name":"Russinova"},{"last_name":"Sun","full_name":"Sun, L","first_name":"L"}],"publication":"Plant Communications","scopus_import":"1","quality_controlled":"1","article_processing_charge":"Yes","supplementarymaterial":"yes","date_created":"2024-12-04T11:21:16Z","month":"01","publication_status":"published","language":[{"iso":"eng"}],"volume":6,"department":[{"_id":"JiFr"}],"DOAJ_listed":"1","OA_place":"publisher","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"fulldoi":"https://doi.org/10.1016/j.xplc.2024.101181","article_type":"original","has_accepted_license":"1","date_published":"2025-01-13T00:00:00Z","OA_type":"gold","ddc":["580"],"abstract":[{"text":"Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signalling initiation, which requires binding of BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter, ABCB19, functions as a BR exporter, and together with its close homologue, ABCB1, positively regulate BR signalling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 was stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states were determined. In the BL-bound structure, BL was bound to the hydrophobic cavity formed by the transmembrane domain, and triggered local conformational changes. Together, our data provide additional insights into the ABC transporter-mediated BR export.","lang":"eng"}],"file_date_updated":"2025-04-16T09:02:05Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:56:15Z","intvolume":" 6","file":[{"file_size":4443183,"date_created":"2025-04-16T09:02:05Z","file_name":"2025_PlantComm_Wei.pdf","file_id":"19575","success":1,"creator":"dernst","content_type":"application/pdf","date_updated":"2025-04-16T09:02:05Z","relation":"main_file","checksum":"7b0e4511e43cc0da06730c3edb7c1167","access_level":"open_access"}],"issue":"1","external_id":{"isi":["001416757300001"],"pmid":["39497419"]},"dataavailabilitystatement":"The ABCB1 structures in the substrate-unbound state, BL-bound state, and ATP plus BL-bound state have been deposited in the Protein Data Bank under accession codes 8ZPX, 8ZPZ, and 8ZQ4, respectively. The corresponding cryo-EM density maps have been deposited in the Electron Microscopy Data Bank under accession codes EMD-60366, EMD-60369, and EMD-60370, respectively.","publisher":"Cell Press","acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We thank Yaowei Wang, Yongming Luo, and Nemanja Vukašinović (VIB-UGhent, Belgium) for useful discussions and technical support. L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation. No conflict of interest is declared.","citation":{"chicago":"Wei, H, H Zhu, W Ying, H Janssens, M Kvasnica, JM Winne, Y Gao, et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications. Cell Press, 2025. https://doi.org/10.1016/j.xplc.2024.101181.","short":"H. Wei, H. Zhu, W. Ying, H. Janssens, M. Kvasnica, J. Winne, Y. Gao, J. Friml, Q. Ma, S. Tan, X. Liu, E. Russinova, L. Sun, Plant Communications 6 (2025).","mla":"Wei, H., et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications, vol. 6, no. 1, 101181, Cell Press, 2025, doi:10.1016/j.xplc.2024.101181.","ama":"Wei H, Zhu H, Ying W, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 2025;6(1). doi:10.1016/j.xplc.2024.101181","ista":"Wei H, Zhu H, Ying W, Janssens H, Kvasnica M, Winne J, Gao Y, Friml J, Ma Q, Tan S, Liu X, Russinova E, Sun L. 2025. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 6(1), 101181.","ieee":"H. Wei et al., “Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1,” Plant Communications, vol. 6, no. 1. Cell Press, 2025.","apa":"Wei, H., Zhu, H., Ying, W., Janssens, H., Kvasnica, M., Winne, J., … Sun, L. (2025). Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. Cell Press. https://doi.org/10.1016/j.xplc.2024.101181"},"year":"2025","isi":1,"das_tickbox":"1","day":"13","oa_version":"Published Version","status":"public","_id":"18619","researchdata_availability":"yes"},{"publication":"The Plant Cell","author":[{"full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368","first_name":"Michelle C"},{"full_name":"Truckenbrodt, Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","last_name":"Truckenbrodt","first_name":"Sven M"},{"first_name":"Caroline","full_name":"Kreuzinger, Caroline","last_name":"Kreuzinger","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0009-0002-5890-120X","first_name":"Syamala","full_name":"Inumella, Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","last_name":"Inumella"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","last_name":"Vistunou","full_name":"Vistunou, Vitali","first_name":"Vitali"},{"orcid":"0000-0003-1216-9105","first_name":"Christoph M","full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Mojtaba","orcid":"0000-0002-7667-6854","last_name":"Tavakoli","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba"},{"first_name":"Nathalie","last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","full_name":"Agudelo Duenas, Nathalie"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","last_name":"Vorlaufer","full_name":"Vorlaufer, Jakob","orcid":"0009-0000-7590-3501","first_name":"Jakob"},{"last_name":"Jahr","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","orcid":"0000-0003-0201-2315","first_name":"Wiebke"},{"full_name":"Randuch, Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","first_name":"Marek"},{"full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","orcid":"0000-0002-2739-8843","first_name":"Alexander J"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"full_name":"Danzl, Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","orcid":"0000-0001-8559-3973","first_name":"Johann G"}],"corr_author":"1","scopus_import":"1","article_number":"koaf006","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"pmid":1,"oa":1,"title":"Super-resolution expansion microscopy in plant roots","doi":"10.1093/plcell/koaf006","type":"journal_article","month":"04","language":[{"iso":"eng"}],"volume":37,"department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"publication_status":"published","project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"grant_number":"754411","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018"},{"grant_number":"26137","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"grant_number":"W1232-B24","call_identifier":"FWF","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets"}],"related_material":{"record":[{"status":"public","id":"18837","relation":"research_data"},{"status":"public","relation":"earlier_version","id":"18689"}]},"quality_controlled":"1","date_created":"2025-02-05T06:52:06Z","article_processing_charge":"Yes (via OA deal)","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. "}],"file_date_updated":"2025-07-31T07:03:43Z","ec_funded":1,"date_updated":"2026-10-20T09:19:12Z","OA_place":"publisher","ddc":["580"],"OA_type":"hybrid","date_published":"2025-04-01T00:00:00Z","has_accepted_license":"1","PlanS_conform":"1","fulldoi":"https://doi.org/10.1093/plcell/koaf006","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"day":"01","isi":1,"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","year":"2025","citation":{"short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:10.1093/plcell/koaf006.","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University Press, 2025. https://doi.org/10.1093/plcell/koaf006.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006","ieee":"M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006."},"publisher":"Oxford University Press","status":"public","_id":"19003","oa_version":"Published Version","external_id":{"pmid":["39792900"],"isi":["001462763100001"]},"issue":"4","file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2025-07-31T07:03:43Z","access_level":"open_access","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","relation":"main_file","date_created":"2025-07-31T07:03:43Z","file_size":53904111,"file_name":"2025_PlantCell_Gallei.pdf","success":1,"file_id":"20092"}],"intvolume":" 37","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}]},{"abstract":[{"text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena.","lang":"eng"}],"file_date_updated":"2025-11-24T10:55:18Z","ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-22T09:15:44Z","OA_place":"publisher","fulldoi":"https://doi.org/10.1016/j.cell.2025.08.026","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"date_published":"2025-10-30T00:00:00Z","has_accepted_license":"1","OA_type":"hybrid","PlanS_conform":"1","citation":{"short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml, Cell 188 (2025) 6138–6150.e17.","mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell, vol. 188, no. 22, Cell Press, 2025, p. 6138–6150.e17, doi:10.1016/j.cell.2025.08.026.","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell. Cell Press, 2025. https://doi.org/10.1016/j.cell.2025.08.026.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. Cell Press. https://doi.org/10.1016/j.cell.2025.08.026","ieee":"L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism,” Cell, vol. 188, no. 22. Cell Press, p. 6138–6150.e17, 2025.","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J. 2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17.","ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 2025;188(22):6138-6150.e17. doi:10.1016/j.cell.2025.08.026"},"year":"2025","acknowledgement":"We gratefully acknowledge Tongda Xu for experimental, material, and conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at the Institute of Science and Technology Austria (ISTA) for their excellent service and assistance. The research leading to these results has received funding from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003 implemented within the Palacký University Young Researcher Grant.","publisher":"Cell Press","day":"30","isi":1,"oa_version":"Published Version","status":"public","_id":"20656","file":[{"file_size":17825465,"date_created":"2025-11-24T10:55:18Z","file_name":"2025_Cell_Rodriguez.pdf","success":1,"file_id":"20679","creator":"dernst","content_type":"application/pdf","date_updated":"2025-11-24T10:55:18Z","checksum":"8ac396a0806ad7f2e4e7a0c1eed712ce","access_level":"open_access","relation":"main_file"}],"intvolume":" 188","external_id":{"pmid":["41043433"],"isi":["001616077900005"]},"issue":"22","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"author":[{"first_name":"Lesia","orcid":"0000-0002-7244-7237","id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","full_name":"Rodriguez Solovey, Lesia"},{"first_name":"Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler","full_name":"Fiedler, Lukas"},{"full_name":"Zou, Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia"},{"full_name":"Giannini, Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini","first_name":"Caterina"},{"full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline"},{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","full_name":"Randuch, Marek","first_name":"Marek"},{"first_name":"Yongfan","last_name":"Yu","full_name":"Yu, Yongfan"},{"first_name":"Zuzana","orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","last_name":"Gelová","full_name":"Gelová, Zuzana"},{"orcid":"0000-0001-7241-2328","first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","full_name":"Verstraeten, Inge"},{"full_name":"Hajny, Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","last_name":"Hajny","orcid":"0000-0003-2140-7195","first_name":"Jakub"},{"first_name":"Meng","full_name":"Chen, Meng","last_name":"Chen"},{"full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","orcid":"0000-0002-0471-8285","first_name":"Shutang"},{"orcid":"0000-0001-8295-2926","first_name":"Lukas","last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas"},{"first_name":"Lanxin","orcid":"0000-0002-5607-272X","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"first_name":"Maria Mar","full_name":"Marques-Bueno, Maria Mar","last_name":"Marques-Bueno"},{"first_name":"Zainab","full_name":"Quddoos, Zainab","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","last_name":"Quddoos"},{"first_name":"Gergely","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar"},{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"first_name":"Yvon","last_name":"Jaillais","full_name":"Jaillais, Yvon"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"publication":"Cell","corr_author":"1","doi":"10.1016/j.cell.2025.08.026","type":"journal_article","publication_identifier":{"issn":["0092-8674"]},"pmid":1,"title":"ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism","oa":1,"month":"10","volume":188,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"XiFe"}],"publication_status":"published","page":"6138-6150.e17","related_material":{"record":[{"relation":"earlier_version","id":"19399","status":"public"},{"relation":"dissertation_contains","id":"23145","status":"public"}]},"quality_controlled":"1","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123"},{"name":"Cell surface receptor complexes for auxin signaling in plants","_id":"26060676-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 985-2016"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"}],"date_created":"2025-11-19T09:44:31Z","article_processing_charge":"Yes (via OA deal)"},{"oa":1,"title":"Cytosolic Ca2+ as a universal signal for rapid root growth regulation","type":"preprint","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.17.683082"}],"doi":"10.1101/2025.10.17.683082","publication":"bioRxiv","author":[{"first_name":"Marek","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch"},{"last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","full_name":"Kulich, Ivan","first_name":"Ivan"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"full_name":"Huang, Shouguang","last_name":"Huang","first_name":"Shouguang"},{"last_name":"Hedrich","full_name":"Hedrich, Rainer","first_name":"Rainer"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"corr_author":"1","project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"related_material":{"record":[{"status":"public","id":"23145","relation":"dissertation_contains"}]},"article_processing_charge":"No","date_created":"2026-10-19T11:44:48Z","month":"10","publication_status":"draft","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"OA_place":"repository","OA_type":"green","date_published":"2025-10-17T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1101/2025.10.17.683082","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Roots must continuously adapt their growth and have evolved the ability to rapidly respond to diverse environmental and hormonal cues, including the phytohormone auxin. Auxin-induced cellular responses involve membrane depolarization, cytosolic calcium (Ca²⁺) elevation, and extracellular pH increase, but how these processes lead to rapid growth regulation remains unclear. Here, we show that cytosolic Ca²⁺ acts as a universal signal integrating diverse cues for rapid regulation of root growth. Using live imaging, microfluidics, and optogenetics in Arabidopsis, we demonstrate that a swift rise in cytosolic Ca²⁺ is the primary target of auxin signaling affecting growth inhibition. Disruption of Ca²⁺ influx abolishes these responses, whereas light-gated Ca²⁺ influx from the apoplast or endoplasmic reticulum stores inhibits growth. Multiple unrelated stimuli—including auxin, extracellular ATP, RALF peptides, and hydrogen peroxide—converge on this Ca²⁺-dependent mechanism. Cytosolic Ca²⁺ elevation thus represents a necessary and sufficient step for rapid growth inhibition, revealing a unifying principle of root signaling."}],"date_updated":"2026-10-22T09:15:44Z","biorxivid":1,"external_id":{"biorxivid":["10.1101/2025.10.17.683082"]},"day":"17","acknowledgement":"We gratefully acknowledge Dr. Andrea Bleckmann, creator of pBLAX000 vector and Dr. Shiqiang Gao for donation of template used in XXM-ER cloning. This research was supported by the Laboratory Support Facility, the Plant Facility, the IT Facility and the Imaging and Optical Facility of the Institute of Science and Technology Austria. The graphical elements used in Fig. 5 were adapted and modified from Servier, https://smart.servier.com, and are used under CC BY 3.0 licence. The authors acknowledge the use of AI-tools (Microsoft Copilot, Gemini) for language refinement. All content was critically reviewed by authors.\r\n\r\nFunder Information Declared\r\nEuropean Research Council, 101142681 CYNIPS\r\nAustrian Science Fund, P 37051-B","citation":{"ieee":"M. Randuch, I. Kulich, D. Vladimirtsev, S. Huang, R. Hedrich, and J. Friml, “Cytosolic Ca2+ as a universal signal for rapid root growth regulation,” bioRxiv. .","apa":"Randuch, M., Kulich, I., Vladimirtsev, D., Huang, S., Hedrich, R., & Friml, J. (n.d.). Cytosolic Ca2+ as a universal signal for rapid root growth regulation. bioRxiv. https://doi.org/10.1101/2025.10.17.683082","ama":"Randuch M, Kulich I, Vladimirtsev D, Huang S, Hedrich R, Friml J. Cytosolic Ca2+ as a universal signal for rapid root growth regulation. bioRxiv. doi:10.1101/2025.10.17.683082","ista":"Randuch M, Kulich I, Vladimirtsev D, Huang S, Hedrich R, Friml J. Cytosolic Ca2+ as a universal signal for rapid root growth regulation. bioRxiv, 10.1101/2025.10.17.683082.","short":"M. Randuch, I. Kulich, D. Vladimirtsev, S. Huang, R. Hedrich, J. Friml, BioRxiv (n.d.).","mla":"Randuch, Marek, et al. “Cytosolic Ca2+ as a Universal Signal for Rapid Root Growth Regulation.” BioRxiv, doi:10.1101/2025.10.17.683082.","chicago":"Randuch, Marek, Ivan Kulich, Dmitrii Vladimirtsev, Shouguang Huang, Rainer Hedrich, and Jiří Friml. “Cytosolic Ca2+ as a Universal Signal for Rapid Root Growth Regulation.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.17.683082."},"year":"2025","_id":"23174","status":"public","oa_version":"Preprint"}]