[{"day":"01","date_published":"2015-01-01T00:00:00Z","publist_id":"5513","ddc":["580"],"author":[{"full_name":"Šimášková, Mária","first_name":"Mária","last_name":"Šimášková"},{"last_name":"O'Brien","first_name":"José","full_name":"O'Brien, José"},{"full_name":"Khan-Djamei, Mamoona","first_name":"Mamoona","id":"391B5BBC-F248-11E8-B48F-1D18A9856A87","last_name":"Khan-Djamei"},{"first_name":"Giel","full_name":"Van Noorden, Giel","last_name":"Van Noorden"},{"first_name":"Krisztina","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","last_name":"Ötvös"},{"full_name":"Vieten, Anne","first_name":"Anne","last_name":"Vieten"},{"last_name":"De Clercq","first_name":"Inge","full_name":"De Clercq, Inge"},{"first_name":"Johanna","full_name":"Van Haperen, Johanna","last_name":"Van Haperen"},{"last_name":"Cuesta","orcid":"0000-0003-1923-2410","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela","full_name":"Cuesta, Candela"},{"last_name":"Hoyerová","full_name":"Hoyerová, Klára","first_name":"Klára"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"full_name":"Marhavy, Peter","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","last_name":"Marhavy"},{"first_name":"Krzysztof T","full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7263-0560","last_name":"Wabnik"},{"last_name":"Van Breusegem","first_name":"Frank","full_name":"Van Breusegem, Frank"},{"last_name":"Nowack","first_name":"Moritz","full_name":"Nowack, Moritz"},{"first_name":"Angus","full_name":"Murphy, Angus","last_name":"Murphy"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiřĺ","full_name":"Friml, Jiřĺ"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"},{"last_name":"Beeckman","first_name":"Tom","full_name":"Beeckman, Tom"},{"full_name":"Benková, Eva","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","date_created":"2018-12-11T11:53:12Z","ec_funded":1,"publication":"Nature Communications","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication_status":"published","status":"public","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"ama":"Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. 2015;6. doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>","mla":"Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>, vol. 6, 8717, Nature Publishing Group, 2015, doi:<a href=\"https://doi.org/10.1038/ncomms9717\">10.1038/ncomms9717</a>.","ista":"Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT, Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E. 2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 6, 8717.","apa":"Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten, A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>","ieee":"M. Šimášková <i>et al.</i>, “Cytokinin response factors regulate PIN-FORMED auxin transporters,” <i>Nature Communications</i>, vol. 6. Nature Publishing Group, 2015.","chicago":"Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden, Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” <i>Nature Communications</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/ncomms9717\">https://doi.org/10.1038/ncomms9717</a>.","short":"M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten, I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý, K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T. Beeckman, E. Benková, Nature Communications 6 (2015)."},"type":"journal_article","doi":"10.1038/ncomms9717","article_number":"8717","scopus_import":"1","abstract":[{"lang":"eng","text":"Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development."}],"file":[{"file_size":1471217,"date_updated":"2020-07-14T12:45:08Z","file_id":"5358","date_created":"2018-12-12T10:18:36Z","relation":"main_file","creator":"system","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf","checksum":"c2c84bca37401435fedf76bad0ba0579"}],"intvolume":"         6","month":"01","has_accepted_license":"1","oa":1,"volume":6,"project":[{"call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362"},{"grant_number":"I 1774-B16","name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"_id":"1640","title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"date_updated":"2025-09-23T08:24:11Z","pubrep_id":"1020","external_id":{"isi":["000366289800001"]},"oa_version":"Submitted Version","acknowledgement":"This work was supported by the European Research Council Starting Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.), by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF).","year":"2015","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","file_date_updated":"2020-07-14T12:45:08Z","publisher":"Nature Publishing Group"},{"day":"02","issue":"3","date_published":"2015-03-02T00:00:00Z","publist_id":"5254","author":[{"last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","full_name":"Grones, Peter","first_name":"Peter"},{"first_name":"Jiřĺ","full_name":"Friml, Jiřĺ","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2018-12-11T11:54:20Z","corr_author":"1","publication":"Molecular Plant","publication_status":"published","status":"public","article_processing_charge":"No","citation":{"short":"P. Grones, J. Friml, Molecular Plant 8 (2015) 356–358.","chicago":"Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” <i>Molecular Plant</i>. Elsevier, 2015. <a href=\"https://doi.org/10.1016/j.molp.2014.12.013\">https://doi.org/10.1016/j.molp.2014.12.013</a>.","ieee":"P. Grones and J. Friml, “ABP1: Finally docking,” <i>Molecular Plant</i>, vol. 8, no. 3. Elsevier, pp. 356–358, 2015.","apa":"Grones, P., &#38; Friml, J. (2015). ABP1: Finally docking. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2014.12.013\">https://doi.org/10.1016/j.molp.2014.12.013</a>","ista":"Grones P, Friml J. 2015. ABP1: Finally docking. Molecular Plant. 8(3), 356–358.","mla":"Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” <i>Molecular Plant</i>, vol. 8, no. 3, Elsevier, 2015, pp. 356–58, doi:<a href=\"https://doi.org/10.1016/j.molp.2014.12.013\">10.1016/j.molp.2014.12.013</a>.","ama":"Grones P, Friml J. ABP1: Finally docking. <i>Molecular Plant</i>. 2015;8(3):356-358. doi:<a href=\"https://doi.org/10.1016/j.molp.2014.12.013\">10.1016/j.molp.2014.12.013</a>"},"language":[{"iso":"eng"}],"doi":"10.1016/j.molp.2014.12.013","type":"journal_article","scopus_import":"1","month":"03","intvolume":"         8","volume":8,"page":"356 - 358","date_updated":"2025-09-23T07:30:08Z","department":[{"_id":"JiFr"}],"title":"ABP1: Finally docking","_id":"1847","external_id":{"isi":["000350813400003"]},"acknowledgement":"This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP), European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation GAČR (GA13-40637S).","oa_version":"None","isi":1,"year":"2015","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","publisher":"Elsevier"},{"scopus_import":"1","month":"09","intvolume":"      1853","abstract":[{"text":"Cell polarity is a fundamental property of pro- and eukaryotic cells. It is necessary for coordination of cell division, cell morphogenesis and signaling processes. How polarity is generated and maintained is a complex issue governed by interconnected feed-back regulations between small GTPase signaling and membrane tension-based signaling that controls membrane trafficking, and cytoskeleton organization and dynamics. Here, we will review the potential role for calcium as a crucial signal that connects and coordinates the respective processes during polarization processes in plants. This article is part of a Special Issue entitled: 13th European Symposium on Calcium.","lang":"eng"}],"volume":1853,"page":"2168 - 2172","date_updated":"2025-09-23T08:04:01Z","department":[{"_id":"JiFr"}],"_id":"1849","title":"Calcium is an organizer of cell polarity in plants","external_id":{"isi":["000359960400028"]},"acknowledgement":"The contributing authors were supported by the Ghent University Special Research Fund (to E.H.), the Interuniversity Attraction Poles Programme (IAP VI/33 and IUAP P7/29 ‘MARS’), the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), and the Research Foundation Flanders (to S.V.).","oa_version":"None","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"year":"2015","publisher":"Elsevier","quality_controlled":"1","day":"01","date_published":"2015-09-01T00:00:00Z","issue":"9","publist_id":"5252","date_created":"2018-12-11T11:54:21Z","author":[{"last_name":"Himschoot","full_name":"Himschoot, Ellie","first_name":"Ellie"},{"first_name":"Tom","full_name":"Beeckman, Tom","last_name":"Beeckman"},{"first_name":"Jiřĺ","full_name":"Friml, Jiřĺ","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"}],"publication":"Biochimica et Biophysica Acta - Molecular Cell Research","publication_status":"published","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"chicago":"Himschoot, Ellie, Tom Beeckman, Jiří Friml, and Steffen Vanneste. “Calcium Is an Organizer of Cell Polarity in Plants.” <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>. Elsevier, 2015. <a href=\"https://doi.org/10.1016/j.bbamcr.2015.02.017\">https://doi.org/10.1016/j.bbamcr.2015.02.017</a>.","short":"E. Himschoot, T. Beeckman, J. Friml, S. Vanneste, Biochimica et Biophysica Acta - Molecular Cell Research 1853 (2015) 2168–2172.","apa":"Himschoot, E., Beeckman, T., Friml, J., &#38; Vanneste, S. (2015). Calcium is an organizer of cell polarity in plants. <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bbamcr.2015.02.017\">https://doi.org/10.1016/j.bbamcr.2015.02.017</a>","ieee":"E. Himschoot, T. Beeckman, J. Friml, and S. Vanneste, “Calcium is an organizer of cell polarity in plants,” <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>, vol. 1853, no. 9. Elsevier, pp. 2168–2172, 2015.","mla":"Himschoot, Ellie, et al. “Calcium Is an Organizer of Cell Polarity in Plants.” <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>, vol. 1853, no. 9, Elsevier, 2015, pp. 2168–72, doi:<a href=\"https://doi.org/10.1016/j.bbamcr.2015.02.017\">10.1016/j.bbamcr.2015.02.017</a>.","ista":"Himschoot E, Beeckman T, Friml J, Vanneste S. 2015. Calcium is an organizer of cell polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research. 1853(9), 2168–2172.","ama":"Himschoot E, Beeckman T, Friml J, Vanneste S. Calcium is an organizer of cell polarity in plants. <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>. 2015;1853(9):2168-2172. doi:<a href=\"https://doi.org/10.1016/j.bbamcr.2015.02.017\">10.1016/j.bbamcr.2015.02.017</a>"},"status":"public","doi":"10.1016/j.bbamcr.2015.02.017","type":"journal_article"},{"publisher":"Company of Biologists","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"year":"2015","acknowledgement":"W.G. is a post-doctoral fellow of the Research Foundation Flanders. H.S.R. is supported by Employment of Best Young Scientists for International Cooperation Empowerment [CZ.1.07/2.3.00/30.0037], co-financed by the European Social Fund and the state budget of the Czech Republic. Mi.S. was funded by the Ramón y Cajal program. This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP], project ‘CEITEC – Central European Institute of Technology’ [CZ.1.05/1.1.00/02.0068], the European Social Fund [CZ.1.07/2.3.00/20.0043] and the Czech Science Foundation GACR [GA13-40637S] to J.F. We acknowledge funding from the Biological and Biotechnological Science Research Council (BBSRC) and Engineering Physics Science Research Council (EPSRC) to R.S. and M.B","oa_version":"None","external_id":{"isi":["000351697100011"]},"date_updated":"2025-09-23T09:50:13Z","department":[{"_id":"JiFr"}],"title":"Plant embryogenesis requires AUX/LAX-mediated auxin influx","_id":"1865","project":[{"call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}],"page":"702 - 711","volume":142,"month":"02","intvolume":"       142","abstract":[{"text":"The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis and subsequent development of the body plan. Although the role of PIN auxin efflux transporters has been clearly assigned during embryonic shoot and root specification, the role of the auxin influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here, we used chemical and genetic tools on Brassica napus microspore-derived embryos and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and LAX2 are required for both shoot and root pole formation, in concert with PIN efflux carriers. Furthermore, we uncovered a positive-feedback loop betweenMONOPTEROS(ARF5)-dependent auxin signalling and auxin transport. ThisMONOPTEROSdependent transcriptional regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4) carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip. These results indicate that auxin-dependent cell specification during embryo development requires balanced auxin transport involving both influx and efflux mechanisms, and that this transport is maintained by a positive transcriptional feedback on auxin signalling.","lang":"eng"}],"scopus_import":"1","doi":"10.1242/dev.115832","type":"journal_article","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"mla":"Robert, Hélène, et al. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin Influx.” <i>Development</i>, vol. 142, no. 4, Company of Biologists, 2015, pp. 702–11, doi:<a href=\"https://doi.org/10.1242/dev.115832\">10.1242/dev.115832</a>.","ista":"Robert H, Grunewald W, Sauer M, Cannoot B, Soriano M, Swarup R, Weijers D, Bennett M, Boutilier K, Friml J. 2015. Plant embryogenesis requires AUX/LAX-mediated auxin influx. Development. 142(4), 702–711.","ama":"Robert H, Grunewald W, Sauer M, et al. Plant embryogenesis requires AUX/LAX-mediated auxin influx. <i>Development</i>. 2015;142(4):702-711. doi:<a href=\"https://doi.org/10.1242/dev.115832\">10.1242/dev.115832</a>","chicago":"Robert, Hélène, Wim Grunewald, Michael Sauer, Bernard Cannoot, Mercedes Soriano, Ranjan Swarup, Dolf Weijers, Malcolm Bennett, Kim Boutilier, and Jiří Friml. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin Influx.” <i>Development</i>. Company of Biologists, 2015. <a href=\"https://doi.org/10.1242/dev.115832\">https://doi.org/10.1242/dev.115832</a>.","short":"H. Robert, W. Grunewald, M. Sauer, B. Cannoot, M. Soriano, R. Swarup, D. Weijers, M. Bennett, K. Boutilier, J. Friml, Development 142 (2015) 702–711.","apa":"Robert, H., Grunewald, W., Sauer, M., Cannoot, B., Soriano, M., Swarup, R., … Friml, J. (2015). Plant embryogenesis requires AUX/LAX-mediated auxin influx. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.115832\">https://doi.org/10.1242/dev.115832</a>","ieee":"H. Robert <i>et al.</i>, “Plant embryogenesis requires AUX/LAX-mediated auxin influx,” <i>Development</i>, vol. 142, no. 4. Company of Biologists, pp. 702–711, 2015."},"status":"public","publication_status":"published","publication":"Development","ec_funded":1,"corr_author":"1","date_created":"2018-12-11T11:54:26Z","author":[{"last_name":"Robert","full_name":"Robert, Hélène","first_name":"Hélène"},{"last_name":"Grunewald","first_name":"Wim","full_name":"Grunewald, Wim"},{"first_name":"Michael","full_name":"Sauer, Michael","last_name":"Sauer"},{"first_name":"Bernard","full_name":"Cannoot, Bernard","last_name":"Cannoot"},{"last_name":"Soriano","first_name":"Mercedes","full_name":"Soriano, Mercedes"},{"first_name":"Ranjan","full_name":"Swarup, Ranjan","last_name":"Swarup"},{"first_name":"Dolf","full_name":"Weijers, Dolf","last_name":"Weijers"},{"full_name":"Bennett, Malcolm","first_name":"Malcolm","last_name":"Bennett"},{"first_name":"Kim","full_name":"Boutilier, Kim","last_name":"Boutilier"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"}],"publist_id":"5231","date_published":"2015-02-15T00:00:00Z","issue":"4","day":"15"},{"volume":128,"oa":1,"page":"1 - 7","_id":"1871","title":"Auxin transporters and binding proteins at a glance","department":[{"_id":"JiFr"}],"date_updated":"2025-09-23T07:58:34Z","scopus_import":"1","intvolume":"       128","month":"01","has_accepted_license":"1","abstract":[{"lang":"eng","text":"The plant hormone auxin is a key regulator of plant growth and development. Differences in auxin distribution within tissues are mediated by the polar auxin transport machinery, and cellular auxin responses occur depending on changes in cellular auxin levels. Multiple receptor systems at the cell surface and in the interior operate to sense and interpret fluctuations in auxin distribution that occur during plant development. Until now, three proteins or protein complexes that can bind auxin have been identified. SCFTIR1 [a SKP1-cullin-1-F-box complex that contains transport inhibitor response 1 (TIR1) as the F-box protein] and S-phase-kinaseassociated protein 2 (SKP2) localize to the nucleus, whereas auxinbinding protein 1 (ABP1), predominantly associates with the endoplasmic reticulum and cell surface. In this Cell Science at a Glance article, we summarize recent discoveries in the field of auxin transport and signaling that have led to the identification of new components of these pathways, as well as their mutual interaction."}],"file":[{"date_updated":"2020-07-14T12:45:19Z","file_id":"4852","date_created":"2018-12-12T10:11:00Z","relation":"main_file","file_size":1688844,"file_name":"IST-2016-563-v1+1_1.full.pdf","checksum":"24c779f4cd9d549ca6833e26f486be27","creator":"system","access_level":"open_access","content_type":"application/pdf"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2015","isi":1,"file_date_updated":"2020-07-14T12:45:19Z","publisher":"Company of Biologists","quality_controlled":"1","external_id":{"isi":["000347167200001"]},"pubrep_id":"563","oa_version":"Submitted Version","acknowledgement":"This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP]; European Social Fund [grant number CZ.1.07/2.3.00/20.0043] and the Czech Science Foundation GAČR [grant number GA13-40637S]","ddc":["570"],"publist_id":"5225","date_created":"2018-12-11T11:54:28Z","corr_author":"1","author":[{"id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","full_name":"Grones, Peter","first_name":"Peter"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","full_name":"Friml, Jirí"}],"day":"01","date_published":"2015-01-01T00:00:00Z","issue":"1","language":[{"iso":"eng"}],"article_processing_charge":"No","citation":{"ama":"Grones P, Friml J. Auxin transporters and binding proteins at a glance. <i>Journal of Cell Science</i>. 2015;128(1):1-7. doi:<a href=\"https://doi.org/10.1242/jcs.159418\">10.1242/jcs.159418</a>","mla":"Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins at a Glance.” <i>Journal of Cell Science</i>, vol. 128, no. 1, Company of Biologists, 2015, pp. 1–7, doi:<a href=\"https://doi.org/10.1242/jcs.159418\">10.1242/jcs.159418</a>.","ista":"Grones P, Friml J. 2015. Auxin transporters and binding proteins at a glance. Journal of Cell Science. 128(1), 1–7.","apa":"Grones, P., &#38; Friml, J. (2015). Auxin transporters and binding proteins at a glance. <i>Journal of Cell Science</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.159418\">https://doi.org/10.1242/jcs.159418</a>","ieee":"P. Grones and J. Friml, “Auxin transporters and binding proteins at a glance,” <i>Journal of Cell Science</i>, vol. 128, no. 1. Company of Biologists, pp. 1–7, 2015.","chicago":"Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins at a Glance.” <i>Journal of Cell Science</i>. Company of Biologists, 2015. <a href=\"https://doi.org/10.1242/jcs.159418\">https://doi.org/10.1242/jcs.159418</a>.","short":"P. Grones, J. Friml, Journal of Cell Science 128 (2015) 1–7."},"status":"public","type":"journal_article","doi":"10.1242/jcs.159418","publication":"Journal of Cell Science","publication_status":"published"},{"abstract":[{"text":"Petrocoptis is a small genus of chasmophytic plants endemic to the Iberian Peninsula, with some localized populations in the French Pyrenees. Within the genus, a dozen species have been recognized based on morphological diversity, most of them with limited distribution area, in small populations and frequently with potential threats to their survival. To date, however, a molecular evaluation of the current systematic treatments has not been carried out. The aim of the present study is to infer phylogenetic relationships among its subordinate taxa by using plastidial rps16 intron and nuclear internal transcribed spacer (ITS) DNA sequences; and evaluate the phylogenetic placement of the genus Petrocoptis within the family Caryophyllaceae. The monophyly of Petrocoptis is supported by both ITS and rps16 intron sequence analyses. Furthermore, time estimates using BEAST analyses indicate a Middle to Late Miocene diversification (10.59 Myr, 6.44–15.26 Myr highest posterior densities [HPD], for ITS; 14.30 Myr, 8.61–21.00 Myr HPD, for rps16 intron).","lang":"eng"}],"intvolume":"       128","month":"01","scopus_import":"1","title":"Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula","_id":"1878","department":[{"_id":"JiFr"}],"date_updated":"2025-09-23T09:49:04Z","page":"223 - 238","volume":128,"oa_version":"None","external_id":{"isi":["000350826000001"]},"quality_controlled":"1","publisher":"Springer","year":"2015","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"2","date_published":"2015-01-24T00:00:00Z","day":"24","author":[{"last_name":"Cires Rodriguez","id":"2AD56A7A-F248-11E8-B48F-1D18A9856A87","first_name":"Eduardo","full_name":"Cires Rodriguez, Eduardo"},{"last_name":"Prieto","full_name":"Prieto, José","first_name":"José"}],"corr_author":"1","date_created":"2018-12-11T11:54:30Z","publist_id":"5217","publication_status":"published","publication":"Journal of Plant Research","type":"journal_article","doi":"10.1007/s10265-014-0691-6","status":"public","language":[{"iso":"eng"}],"citation":{"ama":"Cires Rodriguez E, Prieto J. Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. <i>Journal of Plant Research</i>. 2015;128(2):223-238. doi:<a href=\"https://doi.org/10.1007/s10265-014-0691-6\">10.1007/s10265-014-0691-6</a>","mla":"Cires Rodriguez, Eduardo, and José Prieto. “Phylogenetic Relationships of Petrocoptis A. Braun Ex Endl. (Caryophyllaceae), a Discussed Genus from the Iberian Peninsula.” <i>Journal of Plant Research</i>, vol. 128, no. 2, Springer, 2015, pp. 223–38, doi:<a href=\"https://doi.org/10.1007/s10265-014-0691-6\">10.1007/s10265-014-0691-6</a>.","ista":"Cires Rodriguez E, Prieto J. 2015. Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. Journal of Plant Research. 128(2), 223–238.","apa":"Cires Rodriguez, E., &#38; Prieto, J. (2015). Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. <i>Journal of Plant Research</i>. Springer. <a href=\"https://doi.org/10.1007/s10265-014-0691-6\">https://doi.org/10.1007/s10265-014-0691-6</a>","ieee":"E. Cires Rodriguez and J. Prieto, “Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula,” <i>Journal of Plant Research</i>, vol. 128, no. 2. Springer, pp. 223–238, 2015.","chicago":"Cires Rodriguez, Eduardo, and José Prieto. “Phylogenetic Relationships of Petrocoptis A. Braun Ex Endl. (Caryophyllaceae), a Discussed Genus from the Iberian Peninsula.” <i>Journal of Plant Research</i>. Springer, 2015. <a href=\"https://doi.org/10.1007/s10265-014-0691-6\">https://doi.org/10.1007/s10265-014-0691-6</a>.","short":"E. Cires Rodriguez, J. Prieto, Journal of Plant Research 128 (2015) 223–238."},"article_processing_charge":"No"},{"doi":"10.1111/jmi.12211","type":"journal_article","article_processing_charge":"No","citation":{"chicago":"Kremer, A, Stefaan Lippens, Sonia Bartunkova, Bob Asselbergh, Cendric Blanpain, Matyas Fendrych, A Goossens, et al. “Developing 3D SEM in a Broad Biological Context.” <i>Journal of Microscopy</i>. Wiley-Blackwell, 2015. <a href=\"https://doi.org/10.1111/jmi.12211\">https://doi.org/10.1111/jmi.12211</a>.","short":"A. Kremer, S. Lippens, S. Bartunkova, B. Asselbergh, C. Blanpain, M. Fendrych, A. Goossens, M. Holt, S. Janssens, M. Krols, J. Larsimont, C. Mc Guire, M. Nowack, X. Saelens, A. Schertel, B. Schepens, M. Slezak, V. Timmerman, C. Theunis, R. Van Brempt, Y. Visser, C. Guérin, Journal of Microscopy 259 (2015) 80–96.","apa":"Kremer, A., Lippens, S., Bartunkova, S., Asselbergh, B., Blanpain, C., Fendrych, M., … Guérin, C. (2015). Developing 3D SEM in a broad biological context. <i>Journal of Microscopy</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/jmi.12211\">https://doi.org/10.1111/jmi.12211</a>","ieee":"A. Kremer <i>et al.</i>, “Developing 3D SEM in a broad biological context,” <i>Journal of Microscopy</i>, vol. 259, no. 2. Wiley-Blackwell, pp. 80–96, 2015.","mla":"Kremer, A., et al. “Developing 3D SEM in a Broad Biological Context.” <i>Journal of Microscopy</i>, vol. 259, no. 2, Wiley-Blackwell, 2015, pp. 80–96, doi:<a href=\"https://doi.org/10.1111/jmi.12211\">10.1111/jmi.12211</a>.","ista":"Kremer A, Lippens S, Bartunkova S, Asselbergh B, Blanpain C, Fendrych M, Goossens A, Holt M, Janssens S, Krols M, Larsimont J, Mc Guire C, Nowack M, Saelens X, Schertel A, Schepens B, Slezak M, Timmerman V, Theunis C, Van Brempt R, Visser Y, Guérin C. 2015. Developing 3D SEM in a broad biological context. Journal of Microscopy. 259(2), 80–96.","ama":"Kremer A, Lippens S, Bartunkova S, et al. Developing 3D SEM in a broad biological context. <i>Journal of Microscopy</i>. 2015;259(2):80-96. doi:<a href=\"https://doi.org/10.1111/jmi.12211\">10.1111/jmi.12211</a>"},"language":[{"iso":"eng"}],"status":"public","publication_status":"published","publication":"Journal of Microscopy","date_created":"2018-12-11T11:54:30Z","author":[{"last_name":"Kremer","full_name":"Kremer, A","first_name":"A"},{"full_name":"Lippens, Stefaan","first_name":"Stefaan","last_name":"Lippens"},{"last_name":"Bartunkova","full_name":"Bartunkova, Sonia","first_name":"Sonia"},{"first_name":"Bob","full_name":"Asselbergh, Bob","last_name":"Asselbergh"},{"last_name":"Blanpain","full_name":"Blanpain, Cendric","first_name":"Cendric"},{"full_name":"Fendrych, Matyas","first_name":"Matyas","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych"},{"full_name":"Goossens, A","first_name":"A","last_name":"Goossens"},{"last_name":"Holt","full_name":"Holt, Matthew","first_name":"Matthew"},{"last_name":"Janssens","full_name":"Janssens, Sophie","first_name":"Sophie"},{"full_name":"Krols, Michiel","first_name":"Michiel","last_name":"Krols"},{"last_name":"Larsimont","first_name":"Jean","full_name":"Larsimont, Jean"},{"first_name":"Conor","full_name":"Mc Guire, Conor","last_name":"Mc Guire"},{"last_name":"Nowack","first_name":"Moritz","full_name":"Nowack, Moritz"},{"full_name":"Saelens, Xavier","first_name":"Xavier","last_name":"Saelens"},{"last_name":"Schertel","full_name":"Schertel, Andreas","first_name":"Andreas"},{"first_name":"B","full_name":"Schepens, B","last_name":"Schepens"},{"last_name":"Slezak","full_name":"Slezak, M","first_name":"M"},{"first_name":"Vincent","full_name":"Timmerman, Vincent","last_name":"Timmerman"},{"last_name":"Theunis","full_name":"Theunis, Clara","first_name":"Clara"},{"last_name":"Van Brempt","full_name":"Van Brempt, Ronald","first_name":"Ronald"},{"last_name":"Visser","full_name":"Visser, Y","first_name":"Y"},{"first_name":"Christophe","full_name":"Guérin, Christophe","last_name":"Guérin"}],"ddc":["570"],"publist_id":"5218","date_published":"2015-08-01T00:00:00Z","issue":"2","day":"01","publisher":"Wiley-Blackwell","file_date_updated":"2020-07-14T12:45:19Z","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2015","acknowledgement":"The Zeiss Merlin with Gatan 3View2XP and Zeiss Auriga were acquired through a CLEM grant from Minister Ingrid Lieten to the VIB Bio-Imaging-Core. Michiel Krols and Saskia Lippens are the recipients of a fellowship from the FWO (Fonds Wetenschappelijk Onderzoek) of Flanders.","oa_version":"Published Version","external_id":{"isi":["000358300600002"]},"pubrep_id":"459","date_updated":"2025-09-23T08:26:17Z","department":[{"_id":"JiFr"}],"_id":"1879","title":"Developing 3D SEM in a broad biological context","oa":1,"page":"80 - 96","volume":259,"month":"08","has_accepted_license":"1","intvolume":"       259","file":[{"file_name":"IST-2016-459-v1+1_KREMER_et_al-2015-Journal_of_Microscopy.pdf","checksum":"3649c5372d1644062d728ea9287e367f","access_level":"open_access","content_type":"application/pdf","creator":"system","relation":"main_file","date_created":"2018-12-12T10:11:19Z","date_updated":"2020-07-14T12:45:19Z","file_id":"4872","file_size":2899898}],"abstract":[{"lang":"eng","text":"When electron microscopy (EM) was introduced in the 1930s it gave scientists their first look into the nanoworld of cells. Over the last 80 years EM has vastly increased our understanding of the complex cellular structures that underlie the diverse functions that cells need to maintain life. One drawback that has been difficult to overcome was the inherent lack of volume information, mainly due to the limit on the thickness of sections that could be viewed in a transmission electron microscope (TEM). For many years scientists struggled to achieve three-dimensional (3D) EM using serial section reconstructions, TEM tomography, and scanning EM (SEM) techniques such as freeze-fracture. Although each technique yielded some special information, they required a significant amount of time and specialist expertise to obtain even a very small 3D EM dataset. Almost 20 years ago scientists began to exploit SEMs to image blocks of embedded tissues and perform serial sectioning of these tissues inside the SEM chamber. Using first focused ion beams (FIB) and subsequently robotic ultramicrotomes (serial block-face, SBF-SEM) microscopists were able to collect large volumes of 3D EM information at resolutions that could address many important biological questions, and do so in an efficient manner. We present here some examples of 3D EM taken from the many diverse specimens that have been imaged in our core facility. We propose that the next major step forward will be to efficiently correlate functional information obtained using light microscopy (LM) with 3D EM datasets to more completely investigate the important links between cell structures and their functions."}],"scopus_import":"1"},{"publisher":"Elsevier","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"year":"2015","acknowledgement":"This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP); the Agency for Innovation by Science and Technology (IWT) (predoctoral fellowship to H.R.); and the People Programme (Marie Curie Actions) of the European Union","oa_version":"None","external_id":{"isi":["000349880900017"]},"date_updated":"2025-09-23T09:23:09Z","department":[{"_id":"JiFr"}],"title":"Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants","_id":"1944","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","call_identifier":"FP7"},{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"page":"116 - 123","volume":23,"month":"02","intvolume":"        23","scopus_import":"1","doi":"10.1016/j.pbi.2014.12.002","type":"journal_article","citation":{"ama":"Rakusová H, Fendrych M, Friml J. Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants. <i>Current Opinion in Plant Biology</i>. 2015;23(2):116-123. doi:<a href=\"https://doi.org/10.1016/j.pbi.2014.12.002\">10.1016/j.pbi.2014.12.002</a>","mla":"Rakusová, Hana, et al. “Intracellular Trafficking and PIN-Mediated Cell Polarity during Tropic Responses in Plants.” <i>Current Opinion in Plant Biology</i>, vol. 23, no. 2, Elsevier, 2015, pp. 116–23, doi:<a href=\"https://doi.org/10.1016/j.pbi.2014.12.002\">10.1016/j.pbi.2014.12.002</a>.","ista":"Rakusová H, Fendrych M, Friml J. 2015. Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants. Current Opinion in Plant Biology. 23(2), 116–123.","apa":"Rakusová, H., Fendrych, M., &#38; Friml, J. (2015). Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2014.12.002\">https://doi.org/10.1016/j.pbi.2014.12.002</a>","ieee":"H. Rakusová, M. Fendrych, and J. Friml, “Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants,” <i>Current Opinion in Plant Biology</i>, vol. 23, no. 2. Elsevier, pp. 116–123, 2015.","chicago":"Rakusová, Hana, Matyas Fendrych, and Jiří Friml. “Intracellular Trafficking and PIN-Mediated Cell Polarity during Tropic Responses in Plants.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2015. <a href=\"https://doi.org/10.1016/j.pbi.2014.12.002\">https://doi.org/10.1016/j.pbi.2014.12.002</a>.","short":"H. Rakusová, M. Fendrych, J. Friml, Current Opinion in Plant Biology 23 (2015) 116–123."},"language":[{"iso":"eng"}],"article_processing_charge":"No","status":"public","publication_status":"published","publication":"Current Opinion in Plant Biology","ec_funded":1,"corr_author":"1","date_created":"2018-12-11T11:54:51Z","author":[{"first_name":"Hana","full_name":"Rakusová, Hana","last_name":"Rakusová"},{"last_name":"Fendrych","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas","full_name":"Fendrych, Matyas"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"}],"publist_id":"5140","date_published":"2015-02-01T00:00:00Z","issue":"2","day":"01"},{"year":"2015","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","publisher":"Cell Press","external_id":{"isi":["000364828900020"]},"oa_version":"None","volume":163,"page":"670 - 683","_id":"532","title":"EIN2-directed translational regulation of ethylene signaling in arabidopsis","department":[{"_id":"JiFr"}],"date_updated":"2025-09-23T08:40:43Z","scopus_import":"1","abstract":[{"text":"Ethylene is a gaseous phytohormone that plays vital roles in plant growth and development. Previous studies uncovered EIN2 as an essential signal transducer linking ethylene perception on ER to transcriptional regulation in the nucleus through a “cleave and shuttle” model. In this study, we report another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA. We find that the EBF1/2 3′ UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3′ UTRs. Furthermore, we demonstrate that ethylene induces EIN2 to associate with 3′ UTRs and target EBF1/2 mRNA to cytoplasmic processing-body (P-body) through interacting with multiple P-body factors, including EIN5 and PABs. Our study illustrates translational regulation as a key step in ethylene signaling and presents mRNA 3′ UTR functioning as a “signal transducer” to sense and relay cellular signaling in plants.","lang":"eng"}],"intvolume":"       163","month":"10","status":"public","article_processing_charge":"No","citation":{"mla":"Li, Wenyang, et al. “EIN2-Directed Translational Regulation of Ethylene Signaling in Arabidopsis.” <i>Cell</i>, vol. 163, no. 3, Cell Press, 2015, pp. 670–83, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">10.1016/j.cell.2015.09.037</a>.","ista":"Li W, Ma M, Feng Y, Li H, Wang Y, Ma Y, Li M, An F, Guo H. 2015. EIN2-directed translational regulation of ethylene signaling in arabidopsis. Cell. 163(3), 670–683.","ama":"Li W, Ma M, Feng Y, et al. EIN2-directed translational regulation of ethylene signaling in arabidopsis. <i>Cell</i>. 2015;163(3):670-683. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">10.1016/j.cell.2015.09.037</a>","chicago":"Li, Wenyang, Mengdi Ma, Ying Feng, Hongjiang Li, Yichuan Wang, Yutong Ma, Mingzhe Li, Fengying An, and Hongwei Guo. “EIN2-Directed Translational Regulation of Ethylene Signaling in Arabidopsis.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">https://doi.org/10.1016/j.cell.2015.09.037</a>.","short":"W. Li, M. Ma, Y. Feng, H. Li, Y. Wang, Y. Ma, M. Li, F. An, H. Guo, Cell 163 (2015) 670–683.","apa":"Li, W., Ma, M., Feng, Y., Li, H., Wang, Y., Ma, Y., … Guo, H. (2015). EIN2-directed translational regulation of ethylene signaling in arabidopsis. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">https://doi.org/10.1016/j.cell.2015.09.037</a>","ieee":"W. Li <i>et al.</i>, “EIN2-directed translational regulation of ethylene signaling in arabidopsis,” <i>Cell</i>, vol. 163, no. 3. Cell Press, pp. 670–683, 2015."},"language":[{"iso":"eng"}],"type":"journal_article","doi":"10.1016/j.cell.2015.09.037","publication":"Cell","publication_status":"published","publist_id":"7285","author":[{"last_name":"Li","full_name":"Li, Wenyang","first_name":"Wenyang"},{"last_name":"Ma","first_name":"Mengdi","full_name":"Ma, Mengdi"},{"last_name":"Feng","full_name":"Feng, Ying","first_name":"Ying"},{"first_name":"Hongjiang","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","last_name":"Li"},{"last_name":"Wang","full_name":"Wang, Yichuan","first_name":"Yichuan"},{"full_name":"Ma, Yutong","first_name":"Yutong","last_name":"Ma"},{"first_name":"Mingzhe","full_name":"Li, Mingzhe","last_name":"Li"},{"first_name":"Fengying","full_name":"An, Fengying","last_name":"An"},{"last_name":"Guo","full_name":"Guo, Hongwei","first_name":"Hongwei"}],"date_created":"2018-12-11T11:47:00Z","day":"22","issue":"3","date_published":"2015-10-22T00:00:00Z"},{"external_id":{"isi":["000359136100006"]},"acknowledgement":"This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP); European Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science Foundation GAČR (GA13-40637S) to J.F.; project Postdoc I. (CZ.1.07/2.3.00/30.0009) co-financed by the European Social Fund and the state budget of the Czech Republic to M.Z. and T.N..","oa_version":"None","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"year":"2015","publisher":"Elsevier","quality_controlled":"1","scopus_import":"1","month":"08","intvolume":"         8","abstract":[{"lang":"eng","text":"The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity."}],"project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"volume":8,"page":"1175 - 1187","date_updated":"2025-09-23T09:47:17Z","department":[{"_id":"JiFr"}],"title":"Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana","_id":"1819","publication":"Molecular Plant","ec_funded":1,"publication_status":"published","language":[{"iso":"eng"}],"citation":{"ama":"Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana. <i>Molecular Plant</i>. 2015;8(8):1175-1187. doi:<a href=\"https://doi.org/10.1016/j.molp.2015.03.007\">10.1016/j.molp.2015.03.007</a>","mla":"Zwiewka, Marta, et al. “Osmotic Stress Modulates the Balance between Exocytosis and Clathrin Mediated Endocytosis in Arabidopsis Thaliana.” <i>Molecular Plant</i>, vol. 8, no. 8, Elsevier, 2015, pp. 1175–87, doi:<a href=\"https://doi.org/10.1016/j.molp.2015.03.007\">10.1016/j.molp.2015.03.007</a>.","ista":"Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. 2015. Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana. Molecular Plant. 8(8), 1175–1187.","apa":"Zwiewka, M., Nodzyński, T., Robert, S., Vanneste, S., &#38; Friml, J. (2015). Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2015.03.007\">https://doi.org/10.1016/j.molp.2015.03.007</a>","ieee":"M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, and J. Friml, “Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana,” <i>Molecular Plant</i>, vol. 8, no. 8. Elsevier, pp. 1175–1187, 2015.","chicago":"Zwiewka, Marta, Tomasz Nodzyński, Stéphanie Robert, Steffen Vanneste, and Jiří Friml. “Osmotic Stress Modulates the Balance between Exocytosis and Clathrin Mediated Endocytosis in Arabidopsis Thaliana.” <i>Molecular Plant</i>. Elsevier, 2015. <a href=\"https://doi.org/10.1016/j.molp.2015.03.007\">https://doi.org/10.1016/j.molp.2015.03.007</a>.","short":"M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, J. Friml, Molecular Plant 8 (2015) 1175–1187."},"article_processing_charge":"No","status":"public","doi":"10.1016/j.molp.2015.03.007","type":"journal_article","day":"03","date_published":"2015-08-03T00:00:00Z","issue":"8","publist_id":"5287","corr_author":"1","date_created":"2018-12-11T11:54:11Z","author":[{"first_name":"Marta","full_name":"Zwiewka, Marta","last_name":"Zwiewka"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"full_name":"Friml, Jiřĺ","first_name":"Jiřĺ","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}]},{"ec_funded":1,"publication":"Plant and Cell Physiology","publication_status":"published","status":"public","language":[{"iso":"eng"}],"article_processing_charge":"No","citation":{"ama":"Naramoto S, Nodzyński T, Dainobu T, et al. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):750-763. doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>","ista":"Naramoto S, Nodzyński T, Dainobu T, Takatsuka H, Okada T, Friml J, Fukuda H. 2014. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. Plant and Cell Physiology. 55(4), 750–763.","mla":"Naramoto, Satoshi, et al. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 750–63, doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>.","ieee":"S. Naramoto <i>et al.</i>, “VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 750–763, 2014.","apa":"Naramoto, S., Nodzyński, T., Dainobu, T., Takatsuka, H., Okada, T., Friml, J., &#38; Fukuda, H. (2014). VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>","short":"S. Naramoto, T. Nodzyński, T. Dainobu, H. Takatsuka, T. Okada, J. Friml, H. Fukuda, Plant and Cell Physiology 55 (2014) 750–763.","chicago":"Naramoto, Satoshi, Tomasz Nodzyński, Tomoko Dainobu, Hirotomo Takatsuka, Teruyo Okada, Jiří Friml, and Hiroo Fukuda. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>."},"type":"journal_article","doi":"10.1093/pcp/pcu012","day":"01","issue":"4","date_published":"2014-04-01T00:00:00Z","publist_id":"4742","publication_identifier":{"issn":["0032-0781"]},"author":[{"last_name":"Naramoto","first_name":"Satoshi","full_name":"Naramoto, Satoshi"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"full_name":"Dainobu, Tomoko","first_name":"Tomoko","last_name":"Dainobu"},{"full_name":"Takatsuka, Hirotomo","first_name":"Hirotomo","last_name":"Takatsuka"},{"last_name":"Okada","first_name":"Teruyo","full_name":"Okada, Teruyo"},{"first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Fukuda","first_name":"Hiroo","full_name":"Fukuda, Hiroo"}],"date_created":"2018-12-11T11:56:24Z","external_id":{"isi":["000334679500009"]},"oa_version":"None","year":"2014","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","publisher":"Oxford University Press","scopus_import":"1","abstract":[{"lang":"eng","text":"Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation."}],"intvolume":"        55","month":"04","page":"750 - 763","volume":55,"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7"}],"_id":"2222","title":"VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis","date_updated":"2025-09-29T11:28:27Z","department":[{"_id":"JiFr"}]},{"article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"chicago":"Tanaka, Hirokazu, Tomasz Nodzyński, Saeko Kitakura, Mugurel Feraru, Michiko Sasabe, Tomomi Ishikawa, Jürgen Kleine Vehn, Tatsuo Kakimoto, and Jiří Friml. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>.","short":"H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa, J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.","apa":"Tanaka, H., Nodzyński, T., Kitakura, S., Feraru, M., Sasabe, M., Ishikawa, T., … Friml, J. (2014). BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>","ieee":"H. Tanaka <i>et al.</i>, “BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 737–749, 2014.","mla":"Tanaka, Hirokazu, et al. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 737–49, doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>.","ista":"Tanaka H, Nodzyński T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, Kleine Vehn J, Kakimoto T, Friml J. 2014. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. Plant and Cell Physiology. 55(4), 737–749.","ama":"Tanaka H, Nodzyński T, Kitakura S, et al. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):737-749. doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>"},"status":"public","type":"journal_article","doi":"10.1093/pcp/pct196","publication":"Plant and Cell Physiology","ec_funded":1,"publication_status":"published","ddc":["570"],"publist_id":"4741","publication_identifier":{"issn":["0032-0781"]},"date_created":"2018-12-11T11:56:25Z","author":[{"full_name":"Tanaka, Hirokazu","first_name":"Hirokazu","last_name":"Tanaka"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"full_name":"Kitakura, Saeko","first_name":"Saeko","last_name":"Kitakura"},{"last_name":"Feraru","full_name":"Feraru, Mugurel","first_name":"Mugurel"},{"first_name":"Michiko","full_name":"Sasabe, Michiko","last_name":"Sasabe"},{"first_name":"Tomomi","full_name":"Ishikawa, Tomomi","last_name":"Ishikawa"},{"last_name":"Kleine Vehn","first_name":"Jürgen","full_name":"Kleine Vehn, Jürgen"},{"first_name":"Tatsuo","full_name":"Kakimoto, Tatsuo","last_name":"Kakimoto"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"}],"day":"01","date_published":"2014-04-01T00:00:00Z","issue":"4","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2014","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"isi":1,"pmid":1,"file_date_updated":"2020-07-14T12:45:34Z","publisher":"Oxford University Press","quality_controlled":"1","external_id":{"pmid":["24369434"],"isi":["000334679500008"]},"pubrep_id":"431","oa_version":"Published Version","project":[{"call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"},{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"oa":1,"volume":55,"page":"737 - 749","_id":"2223","title":"BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis","date_updated":"2025-09-29T11:27:52Z","department":[{"_id":"JiFr"}],"scopus_import":"1","intvolume":"        55","has_accepted_license":"1","month":"04","abstract":[{"lang":"eng","text":"Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin is distributed through intercellular transport and triggers various cellular responses. Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA), inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes 19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization have been genetically poorly defined. Using a fluorescence imaging-based forward genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent protein (GFP) as well as development is hypersensitive to BFA. We found that in bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent with the developmental importance of PIN proteins, functional interference with ARF1 resulted in an impaired auxin response gradient and various developmental defects including embryonic patterning defects and growth arrest. Our results show that ARF1A1C is essential for recycling of PIN auxin transporters and for various auxin-dependent developmental processes."}],"file":[{"date_created":"2018-12-12T10:14:25Z","relation":"main_file","file_id":"5076","date_updated":"2020-07-14T12:45:34Z","file_size":2028111,"checksum":"b781a76b32ac35a520256453c3ba9433","file_name":"IST-2016-431-v1+1_Plant_Cell_Physiol-2014-Tanaka-737-49.pdf","content_type":"application/pdf","access_level":"open_access","creator":"system"}]},{"publisher":"Springer","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2014","isi":1,"oa_version":"None","external_id":{"isi":["000332585400001"]},"title":"Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences","_id":"2227","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_updated":"2025-09-29T11:25:37Z","page":"1 - 10","volume":14,"intvolume":"        14","month":"03","abstract":[{"lang":"eng","text":"The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship with the widespread R. parnassiifolius, based on nuclear DNA (internal transcribed spacer, ITS) and plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK and ycf6-psbM). Maximum parsimony and Bayesian inference analyses revealed a well-supported clade formed by accessions of R. wettsteinii. Furthermore, our phylogenetic and network analyses supported previous hypotheses of a likely allopolyploid origin for R. wettsteinii between R. montenegrinus and R. parnassiifolius, with the latter as the maternal parent."}],"scopus_import":"1","type":"journal_article","doi":"10.1007/s13127-013-0150-6","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"mla":"Cires Rodriguez, Eduardo, et al. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1, Springer, 2014, pp. 1–10, doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>.","ista":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. 2014. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. Organisms Diversity and Evolution. 14(1), 1–10.","ama":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. 2014;14(1):1-10. doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>","chicago":"Cires Rodriguez, Eduardo, Matthias Baltisberger, Candela Cuesta, Pablo Vargas, and José Prieto. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>. Springer, 2014. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>.","short":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, J. Prieto, Organisms Diversity and Evolution 14 (2014) 1–10.","apa":"Cires Rodriguez, E., Baltisberger, M., Cuesta, C., Vargas, P., &#38; Prieto, J. (2014). Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. Springer. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>","ieee":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, and J. Prieto, “Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences,” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1. Springer, pp. 1–10, 2014."},"status":"public","publication_status":"published","publication":"Organisms Diversity and Evolution","corr_author":"1","date_created":"2018-12-11T11:56:26Z","author":[{"last_name":"Cires Rodriguez","id":"2AD56A7A-F248-11E8-B48F-1D18A9856A87","first_name":"Eduardo","full_name":"Cires Rodriguez, Eduardo"},{"full_name":"Baltisberger, Matthias","first_name":"Matthias","last_name":"Baltisberger"},{"full_name":"Cuesta, Candela","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1923-2410","last_name":"Cuesta"},{"last_name":"Vargas","first_name":"Pablo","full_name":"Vargas, Pablo"},{"last_name":"Prieto","full_name":"Prieto, José","first_name":"José"}],"publist_id":"4734","publication_identifier":{"issn":["1439-6092"]},"date_published":"2014-03-01T00:00:00Z","issue":"1","day":"01"},{"publication_status":"published","publication":"Cell","type":"journal_article","doi":"10.1016/j.cell.2014.01.039","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"ama":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>","ista":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B, Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S, De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. Cell. 156(4), 691–704.","mla":"Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>.","ieee":"A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704, 2014.","apa":"Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>","short":"A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K. Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke, B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer, T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova, S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.","chicago":"Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>."},"status":"public","date_published":"2014-02-13T00:00:00Z","issue":"4","day":"13","date_created":"2018-12-11T11:56:31Z","author":[{"last_name":"Gadeyne","full_name":"Gadeyne, Astrid","first_name":"Astrid"},{"last_name":"Sánchez Rodríguez","full_name":"Sánchez Rodríguez, Clara","first_name":"Clara"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"first_name":"Simone","full_name":"Di Rubbo, Simone","last_name":"Di Rubbo"},{"last_name":"Zauber","first_name":"Henrik","full_name":"Zauber, Henrik"},{"last_name":"Vanneste","full_name":"Vanneste, Kevin","first_name":"Kevin"},{"first_name":"Jelle","full_name":"Van Leene, Jelle","last_name":"Van Leene"},{"last_name":"De Winne","first_name":"Nancy","full_name":"De Winne, Nancy"},{"first_name":"Dominique","full_name":"Eeckhout, Dominique","last_name":"Eeckhout"},{"first_name":"Geert","full_name":"Persiau, Geert","last_name":"Persiau"},{"last_name":"Van De Slijke","first_name":"Eveline","full_name":"Van De Slijke, Eveline"},{"first_name":"Bernard","full_name":"Cannoot, Bernard","last_name":"Cannoot"},{"full_name":"Vercruysse, Leen","first_name":"Leen","last_name":"Vercruysse"},{"first_name":"Jonathan","full_name":"Mayers, Jonathan","last_name":"Mayers"},{"full_name":"Adamowski, Maciek","first_name":"Maciek","last_name":"Adamowski","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Urszula","full_name":"Kania, Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","last_name":"Kania"},{"full_name":"Ehrlich, Matthias","first_name":"Matthias","last_name":"Ehrlich"},{"last_name":"Schweighofer","first_name":"Alois","full_name":"Schweighofer, Alois"},{"full_name":"Ketelaar, Tijs","first_name":"Tijs","last_name":"Ketelaar"},{"last_name":"Maere","first_name":"Steven","full_name":"Maere, Steven"},{"last_name":"Bednarek","full_name":"Bednarek, Sebastian","first_name":"Sebastian"},{"full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Gevaert","first_name":"Kris","full_name":"Gevaert, Kris"},{"first_name":"Erwin","full_name":"Witters, Erwin","last_name":"Witters"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"},{"first_name":"Staffan","full_name":"Persson, Staffan","last_name":"Persson"},{"full_name":"De Jaeger, Geert","first_name":"Geert","last_name":"De Jaeger"},{"first_name":"Daniël","full_name":"Van Damme, Daniël","last_name":"Van Damme"}],"publist_id":"4721","publication_identifier":{"issn":["0092-8674"]},"oa_version":"None","external_id":{"isi":["000331379800009"]},"publisher":"Cell Press","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2014","isi":1,"intvolume":"       156","month":"02","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis."}],"scopus_import":"1","title":"The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants","_id":"2240","department":[{"_id":"JiFr"}],"date_updated":"2025-09-29T11:20:21Z","page":"691 - 704","volume":156},{"alternative_title":["Methods in Molecular Biology"],"publist_id":"4704","publication_identifier":{"issn":["1064-3745"]},"date_created":"2018-12-11T11:56:32Z","author":[{"full_name":"Simon, Sibu","first_name":"Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon"},{"full_name":"Skůpa, Petr","first_name":"Petr","last_name":"Skůpa"},{"last_name":"Dobrev","full_name":"Dobrev, Petre","first_name":"Petre"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"first_name":"Eva","full_name":"Zažímalová, Eva","last_name":"Zažímalová"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí"}],"day":"01","date_published":"2014-01-01T00:00:00Z","editor":[{"last_name":"Hicks","full_name":"Hicks, Glenn","first_name":"Glenn"},{"first_name":"Stéphanie","full_name":"Robert, Stéphanie","last_name":"Robert"}],"citation":{"short":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.","chicago":"Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>.","ieee":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in <i>Plant Chemical Genomics</i>, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.","apa":"Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., &#38; Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks &#38; S. Robert (Eds.), <i>Plant Chemical Genomics</i> (Vol. 1056, pp. 255–264). Springer. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>","ista":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Plant Chemical Genomics. Methods in Molecular Biology, vol. 1056, 255–264.","mla":"Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>.","ama":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. <i>Plant Chemical Genomics</i>. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>"},"article_processing_charge":"No","language":[{"iso":"eng"}],"status":"public","type":"book_chapter","doi":"10.1007/978-1-62703-592-7_23","series_title":"Methods in Molecular Biology","publication":"Plant Chemical Genomics","publication_status":"published","page":"255 - 264","volume":1056,"title":"Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters","_id":"2245","date_updated":"2025-07-10T11:52:16Z","department":[{"_id":"JiFr"}],"scopus_import":"1","intvolume":"      1056","month":"01","abstract":[{"text":"Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. ","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2014","publisher":"Springer","quality_controlled":"1","oa_version":"None"},{"oa_version":"Submitted Version","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/","open_access":"1"}],"external_id":{"isi":["000328661300008"]},"quality_controlled":"1","publisher":"Wiley-Blackwell","year":"2014","isi":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","abstract":[{"lang":"eng","text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes."}],"intvolume":"        77","month":"01","scopus_import":"1","_id":"2249","title":"Inter-regulation of the unfolded protein response and auxin signaling","department":[{"_id":"JiFr"}],"date_updated":"2026-04-16T10:08:30Z","oa":1,"page":"97 - 107","volume":77,"publication_status":"published","publication":"Plant Journal","type":"journal_article","doi":"10.1111/tpj.12373","status":"public","language":[{"iso":"eng"}],"citation":{"ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014.","apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>.","ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107.","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>."},"article_processing_charge":"No","issue":"1","date_published":"2014-01-01T00:00:00Z","day":"01","author":[{"last_name":"Chen","first_name":"Yani","full_name":"Chen, Yani"},{"last_name":"Aung","full_name":"Aung, Kyaw","first_name":"Kyaw"},{"last_name":"Rolčík","full_name":"Rolčík, Jakub","first_name":"Jakub"},{"full_name":"Walicki, Kathryn","first_name":"Kathryn","last_name":"Walicki"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí"},{"full_name":"Brandizzí, Federica","first_name":"Federica","last_name":"Brandizzí"}],"date_created":"2018-12-11T11:56:34Z","publist_id":"4699","publication_identifier":{"issn":["0960-7412"]}},{"publication_status":"published","publication":"Plant Journal","type":"journal_article","doi":"10.1111/tpj.12369","citation":{"apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118."},"article_processing_charge":"No","language":[{"iso":"eng"}],"status":"public","date_published":"2014-01-01T00:00:00Z","issue":"1","day":"01","date_created":"2018-12-11T11:56:35Z","author":[{"full_name":"Bailly, Aurélien","first_name":"Aurélien","last_name":"Bailly"},{"first_name":"Bangjun","full_name":"Wang, Bangjun","last_name":"Wang"},{"last_name":"Zwiewka","full_name":"Zwiewka, Marta","first_name":"Marta"},{"full_name":"Pollmann, Stephan","first_name":"Stephan","last_name":"Pollmann"},{"first_name":"Daniel","full_name":"Schenck, Daniel","last_name":"Schenck"},{"first_name":"Hartwig","full_name":"Lüthen, Hartwig","last_name":"Lüthen"},{"last_name":"Schulz","first_name":"Alexander","full_name":"Schulz, Alexander"},{"first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"}],"ddc":["580"],"publist_id":"4694","publication_identifier":{"issn":["0960-7412"]},"oa_version":"Published Version","external_id":{"isi":["000328661300009"]},"main_file_link":[{"url":"https://doi.org/10.1111/tpj.12369","open_access":"1"}],"publisher":"Wiley-Blackwell","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2014","isi":1,"intvolume":"        77","article_type":"original","month":"01","abstract":[{"text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.","lang":"eng"}],"scopus_import":"1","_id":"2253","title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","department":[{"_id":"JiFr"}],"date_updated":"2026-06-18T18:33:25Z","project":[{"name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur","_id":"256BDAB0-B435-11E9-9278-68D0E5697425"}],"page":"108 - 118","oa":1,"volume":77},{"day":"16","issue":"APRIL","date_published":"2014-04-16T00:00:00Z","publist_id":"4786","ddc":["570"],"author":[{"first_name":"Urszula","full_name":"Kania, Urszula","last_name":"Kania","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych"},{"first_name":"Jiřĺ","full_name":"Friml, Jiřĺ","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"date_created":"2018-12-11T11:56:13Z","corr_author":"1","publication":"Open Biology","publication_status":"published","status":"public","article_processing_charge":"No","language":[{"iso":"eng"}],"citation":{"chicago":"Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>. Royal Society, 2014. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>.","short":"U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014).","apa":"Kania, U., Fendrych, M., &#38; Friml, J. (2014). Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>","ieee":"U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities and differences to animal epithelial cells,” <i>Open Biology</i>, vol. 4, no. APRIL. Royal Society, 2014.","mla":"Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>, vol. 4, no. APRIL, 140017, Royal Society, 2014, doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>.","ista":"Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.","ama":"Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. 2014;4(APRIL). doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>"},"type":"journal_article","doi":"10.1098/rsob.140017","article_number":"140017","scopus_import":"1","abstract":[{"lang":"eng","text":"Although plant and animal cells use a similar core mechanism to deliver proteins to the plasma membrane, their different lifestyle, body organization and specific cell structures resulted in the acquisition of regulatory mechanisms that vary in the two kingdoms. In particular, cell polarity regulators do not seem to be conserved, because genes encoding key components are absent in plant genomes. In plants, the broad knowledge on polarity derives from the study of auxin transporters, the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals, much information is provided from the study of polarity in epithelial cells that exhibit basolateral and luminal apical polarities, separated by tight junctions. In this review, we summarize the similarities and differences of the polarization mechanisms between plants and animals and survey the main genetic approaches that have been used to characterize new genes involved in polarity establishment in plants, including the frequently used forward and reverse genetics screens as well as a novel chemical genetics approach that is expected to overcome the limitation of classical genetics methods."}],"file":[{"checksum":"2020627feff36cf0799167c84149fa75","file_name":"IST-2016-441-v1+1_140017.full.pdf","creator":"system","content_type":"application/pdf","access_level":"open_access","file_id":"5025","date_updated":"2020-07-14T12:45:31Z","date_created":"2018-12-12T10:13:40Z","relation":"main_file","file_size":682570}],"intvolume":"         4","month":"04","has_accepted_license":"1","oa":1,"volume":4,"_id":"2188","title":"Polar delivery in plants; commonalities and differences to animal epithelial cells","date_updated":"2025-09-29T11:32:17Z","department":[{"_id":"JiFr"}],"pubrep_id":"441","external_id":{"isi":["000335813800006"]},"oa_version":"Published Version","acknowledgement":"This work was supported by a grant from the Research Foundation-Flanders (Odysseus).\r\n\r\n","year":"2014","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","publisher":"Royal Society","file_date_updated":"2020-07-14T12:45:31Z"},{"date_published":"2014-10-06T00:00:00Z","issue":"19","day":"06","date_created":"2018-12-11T11:54:22Z","author":[{"full_name":"Sassi, Massimiliano","first_name":"Massimiliano","last_name":"Sassi"},{"last_name":"Ali","first_name":"Olivier","full_name":"Ali, Olivier"},{"last_name":"Boudon","full_name":"Boudon, Frédéric","first_name":"Frédéric"},{"first_name":"Gladys","full_name":"Cloarec, Gladys","last_name":"Cloarec"},{"last_name":"Abad","first_name":"Ursula","full_name":"Abad, Ursula"},{"full_name":"Cellier, Coralie","first_name":"Coralie","last_name":"Cellier"},{"last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Chen, Xu","first_name":"Xu"},{"full_name":"Gilles, Benjamin","first_name":"Benjamin","last_name":"Gilles"},{"full_name":"Milani, Pascale","first_name":"Pascale","last_name":"Milani"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Vernoux, Teva","first_name":"Teva","last_name":"Vernoux"},{"full_name":"Godin, Christophe","first_name":"Christophe","last_name":"Godin"},{"last_name":"Hamant","full_name":"Hamant, Olivier","first_name":"Olivier"},{"last_name":"Traas","full_name":"Traas, Jan","first_name":"Jan"}],"publist_id":"5248","publication_status":"published","publication":"Current Biology","doi":"10.1016/j.cub.2014.08.036","type":"journal_article","language":[{"iso":"eng"}],"article_processing_charge":"No","citation":{"chicago":"Sassi, Massimiliano, Olivier Ali, Frédéric Boudon, Gladys Cloarec, Ursula Abad, Coralie Cellier, Xu Chen, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>.","short":"M. Sassi, O. Ali, F. Boudon, G. Cloarec, U. Abad, C. Cellier, X. Chen, B. Gilles, P. Milani, J. Friml, T. Vernoux, C. Godin, O. Hamant, J. Traas, Current Biology 24 (2014) 2335–2342.","apa":"Sassi, M., Ali, O., Boudon, F., Cloarec, G., Abad, U., Cellier, C., … Traas, J. (2014). An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>","ieee":"M. Sassi <i>et al.</i>, “An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis,” <i>Current Biology</i>, vol. 24, no. 19. Cell Press, pp. 2335–2342, 2014.","mla":"Sassi, Massimiliano, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>, vol. 24, no. 19, Cell Press, 2014, pp. 2335–42, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>.","ista":"Sassi M, Ali O, Boudon F, Cloarec G, Abad U, Cellier C, Chen X, Gilles B, Milani P, Friml J, Vernoux T, Godin C, Hamant O, Traas J. 2014. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. Current Biology. 24(19), 2335–2342.","ama":"Sassi M, Ali O, Boudon F, et al. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. 2014;24(19):2335-2342. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>"},"status":"public","month":"10","intvolume":"        24","abstract":[{"lang":"eng","text":"To control morphogenesis, molecular regulatory networks have to interfere with the mechanical properties of the individual cells of developing organs and tissues, but how this is achieved is not well known. We study this issue here in the shoot meristem of higher plants, a group of undifferentiated cells where complex changes in growth rates and directions lead to the continuous formation of new organs [1, 2]. Here, we show that the plant hormone auxin plays an important role in this process via a dual, local effect on the extracellular matrix, the cell wall, which determines cell shape. Our study reveals that auxin not only causes a limited reduction in wall stiffness but also directly interferes with wall anisotropy via the regulation of cortical microtubule dynamics. We further show that to induce growth isotropy and organ outgrowth, auxin somehow interferes with the cortical microtubule-ordering activity of a network of proteins, including AUXIN BINDING PROTEIN 1 and KATANIN 1. Numerical simulations further indicate that the induced isotropy is sufficient to amplify the effects of the relatively minor changes in wall stiffness to promote organogenesis and the establishment of new growth axes in a robust manner."}],"scopus_import":"1","date_updated":"2025-09-29T13:10:45Z","department":[{"_id":"JiFr"}],"_id":"1852","title":"An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis","oa":1,"volume":24,"page":"2335 - 2342","acknowledgement":"This work was funded by grants from EraSysBio+ (iSAM) and ERC (Morphodynamics). ","oa_version":"Submitted Version","external_id":{"isi":["000342747600031"]},"main_file_link":[{"url":"https://hal.archives-ouvertes.fr/hal-01074821","open_access":"1"}],"publisher":"Cell Press","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"year":"2014"},{"status":"public","article_processing_charge":"No","citation":{"short":"X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusova, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93.","chicago":"Chen, Xu, Laurie Grandont, Hongjiang Li, Robert Hauschild, Sébastien Paque, Anas Abuzeineh, Hana Rakusova, Eva Benková, Catherine Perrot Rechenmann, and Jiří Friml. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>.","ieee":"X. Chen <i>et al.</i>, “Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules,” <i>Nature</i>, vol. 516, no. 729. Nature Publishing Group, pp. 90–93, 2014.","apa":"Chen, X., Grandont, L., Li, H., Hauschild, R., Paque, S., Abuzeineh, A., … Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>","ista":"Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusova H, Benková E, Perrot Rechenmann C, Friml J. 2014. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 516(729), 90–93.","mla":"Chen, Xu, et al. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>, vol. 516, no. 729, Nature Publishing Group, 2014, pp. 90–93, doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>.","ama":"Chen X, Grandont L, Li H, et al. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. 2014;516(729):90-93. doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>"},"language":[{"iso":"eng"}],"doi":"10.1038/nature13889","type":"journal_article","ec_funded":1,"publication":"Nature","publication_status":"published","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publist_id":"5237","author":[{"full_name":"Chen, Xu","first_name":"Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen"},{"last_name":"Grandont","first_name":"Laurie","full_name":"Grandont, Laurie"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","last_name":"Li","first_name":"Hongjiang","full_name":"Li, Hongjiang"},{"last_name":"Hauschild","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert"},{"last_name":"Paque","full_name":"Paque, Sébastien","first_name":"Sébastien"},{"last_name":"Abuzeineh","full_name":"Abuzeineh, Anas","first_name":"Anas"},{"first_name":"Hana","full_name":"Rakusova, Hana","last_name":"Rakusova","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva"},{"last_name":"Perrot Rechenmann","full_name":"Perrot Rechenmann, Catherine","first_name":"Catherine"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"}],"corr_author":"1","date_created":"2018-12-11T11:54:25Z","day":"04","issue":"729","date_published":"2014-12-04T00:00:00Z","isi":1,"pmid":1,"year":"2014","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","publisher":"Nature Publishing Group","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257754/"}],"external_id":{"isi":["000346310800045"],"pmid":["25409144"]},"acknowledgement":"We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.","oa_version":"Submitted Version","oa":1,"volume":516,"page":"90 - 93","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7"}],"department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"EvBe"}],"date_updated":"2025-09-29T13:10:05Z","_id":"1862","title":"Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules","scopus_import":"1","abstract":[{"lang":"eng","text":"The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses."}],"month":"12","intvolume":"       516","article_type":"original"}]