[{"date_updated":"2025-09-29T11:20:21Z","day":"13","type":"journal_article","status":"public","date_created":"2018-12-11T11:56:31Z","page":"691 - 704","publication":"Cell","publisher":"Cell Press","isi":1,"external_id":{"isi":["000331379800009"]},"doi":"10.1016/j.cell.2014.01.039","volume":156,"language":[{"iso":"eng"}],"scopus_import":"1","abstract":[{"text":"Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.","lang":"eng"}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"2240","title":"The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants","publication_identifier":{"issn":["0092-8674"]},"intvolume":"       156","quality_controlled":"1","department":[{"_id":"JiFr"}],"publist_id":"4721","citation":{"short":"A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K. Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke, B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer, T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova, S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.","chicago":"Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>.","ista":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B, Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S, De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. Cell. 156(4), 691–704.","ama":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>","ieee":"A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704, 2014.","apa":"Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>","mla":"Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>."},"issue":"4","month":"02","author":[{"full_name":"Gadeyne, Astrid","first_name":"Astrid","last_name":"Gadeyne"},{"last_name":"Sánchez Rodríguez","first_name":"Clara","full_name":"Sánchez Rodríguez, Clara"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"full_name":"Di Rubbo, Simone","first_name":"Simone","last_name":"Di Rubbo"},{"full_name":"Zauber, Henrik","last_name":"Zauber","first_name":"Henrik"},{"first_name":"Kevin","last_name":"Vanneste","full_name":"Vanneste, Kevin"},{"full_name":"Van Leene, Jelle","last_name":"Van Leene","first_name":"Jelle"},{"first_name":"Nancy","last_name":"De Winne","full_name":"De Winne, Nancy"},{"first_name":"Dominique","last_name":"Eeckhout","full_name":"Eeckhout, Dominique"},{"last_name":"Persiau","first_name":"Geert","full_name":"Persiau, Geert"},{"last_name":"Van De Slijke","first_name":"Eveline","full_name":"Van De Slijke, Eveline"},{"last_name":"Cannoot","first_name":"Bernard","full_name":"Cannoot, Bernard"},{"first_name":"Leen","last_name":"Vercruysse","full_name":"Vercruysse, Leen"},{"full_name":"Mayers, Jonathan","last_name":"Mayers","first_name":"Jonathan"},{"orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek","last_name":"Adamowski","first_name":"Maciek"},{"full_name":"Kania, Urszula","first_name":"Urszula","last_name":"Kania","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ehrlich, Matthias","last_name":"Ehrlich","first_name":"Matthias"},{"last_name":"Schweighofer","first_name":"Alois","full_name":"Schweighofer, Alois"},{"last_name":"Ketelaar","first_name":"Tijs","full_name":"Ketelaar, Tijs"},{"full_name":"Maere, Steven","first_name":"Steven","last_name":"Maere"},{"first_name":"Sebastian","last_name":"Bednarek","full_name":"Bednarek, Sebastian"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"full_name":"Gevaert, Kris","first_name":"Kris","last_name":"Gevaert"},{"last_name":"Witters","first_name":"Erwin","full_name":"Witters, Erwin"},{"last_name":"Russinova","first_name":"Eugenia","full_name":"Russinova, Eugenia"},{"full_name":"Persson, Staffan","first_name":"Staffan","last_name":"Persson"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"full_name":"Van Damme, Daniël","first_name":"Daniël","last_name":"Van Damme"}],"date_published":"2014-02-13T00:00:00Z","publication_status":"published","oa_version":"None","year":"2014"},{"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. "}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"scopus_import":"1","editor":[{"last_name":"Hicks","first_name":"Glenn","full_name":"Hicks, Glenn"},{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"}],"volume":1056,"doi":"10.1007/978-1-62703-592-7_23","publisher":"Springer","publication":"Plant Chemical Genomics","page":"255 - 264","status":"public","date_created":"2018-12-11T11:56:32Z","date_updated":"2025-07-10T11:52:16Z","type":"book_chapter","series_title":"Methods in Molecular Biology","day":"01","date_published":"2014-01-01T00:00:00Z","year":"2014","publication_status":"published","oa_version":"None","author":[{"orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","full_name":"Simon, Sibu","last_name":"Simon","first_name":"Sibu"},{"full_name":"Skůpa, Petr","first_name":"Petr","last_name":"Skůpa"},{"full_name":"Dobrev, Petre","last_name":"Dobrev","first_name":"Petre"},{"full_name":"Petrášek, Jan","first_name":"Jan","last_name":"Petrášek"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"}],"month":"01","citation":{"chicago":"Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>.","short":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.","ista":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Plant Chemical Genomics. Methods in Molecular Biology, vol. 1056, 255–264.","apa":"Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., &#38; Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks &#38; S. Robert (Eds.), <i>Plant Chemical Genomics</i> (Vol. 1056, pp. 255–264). Springer. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>","ieee":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in <i>Plant Chemical Genomics</i>, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.","mla":"Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>.","ama":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. <i>Plant Chemical Genomics</i>. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>"},"publist_id":"4704","department":[{"_id":"JiFr"}],"intvolume":"      1056","alternative_title":["Methods in Molecular Biology"],"quality_controlled":"1","title":"Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters","_id":"2245","publication_identifier":{"issn":["1064-3745"]}},{"citation":{"apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014.","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>.","ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>.","short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107."},"issue":"1","month":"01","author":[{"last_name":"Chen","first_name":"Yani","full_name":"Chen, Yani"},{"full_name":"Aung, Kyaw","last_name":"Aung","first_name":"Kyaw"},{"first_name":"Jakub","last_name":"Rolčík","full_name":"Rolčík, Jakub"},{"last_name":"Walicki","first_name":"Kathryn","full_name":"Walicki, Kathryn"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"last_name":"Brandizzí","first_name":"Federica","full_name":"Brandizzí, Federica"}],"publication_status":"published","oa_version":"Submitted Version","year":"2014","date_published":"2014-01-01T00:00:00Z","publication_identifier":{"issn":["09607412"]},"_id":"2249","title":"Inter-regulation of the unfolded protein response and auxin signaling","quality_controlled":"1","intvolume":"        77","department":[{"_id":"JiFr"}],"publist_id":"4699","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/","open_access":"1"}],"oa":1,"external_id":{"isi":["000328661300008"]},"doi":"10.1111/tpj.12373","volume":77,"scopus_import":"1","language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes."}],"article_processing_charge":"No","day":"01","type":"journal_article","date_updated":"2025-09-29T11:17:33Z","date_created":"2018-12-11T11:56:34Z","status":"public","page":"97 - 107","isi":1,"publication":"Plant Journal","publisher":"Wiley-Blackwell"},{"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"scopus_import":"1","volume":77,"project":[{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"doi":"10.1111/tpj.12369","external_id":{"isi":["000328661300009"]},"publisher":"Wiley-Blackwell","publication":"Plant Journal","isi":1,"page":"108 - 118","status":"public","date_created":"2018-12-11T11:56:35Z","date_updated":"2025-09-29T11:15:34Z","type":"journal_article","day":"01","date_published":"2014-01-01T00:00:00Z","year":"2014","oa_version":"Published Version","publication_status":"published","article_type":"original","author":[{"first_name":"Aurélien","last_name":"Bailly","full_name":"Bailly, Aurélien"},{"full_name":"Wang, Bangjun","first_name":"Bangjun","last_name":"Wang"},{"full_name":"Zwiewka, Marta","last_name":"Zwiewka","first_name":"Marta"},{"last_name":"Pollmann","first_name":"Stephan","full_name":"Pollmann, Stephan"},{"last_name":"Schenck","first_name":"Daniel","full_name":"Schenck, Daniel"},{"full_name":"Lüthen, Hartwig","first_name":"Hartwig","last_name":"Lüthen"},{"full_name":"Schulz, Alexander","last_name":"Schulz","first_name":"Alexander"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markus","last_name":"Geisler","full_name":"Geisler, Markus"}],"month":"01","citation":{"chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118.","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>"},"issue":"1","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/tpj.12369"}],"publist_id":"4694","department":[{"_id":"JiFr"}],"intvolume":"        77","quality_controlled":"1","title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","_id":"2253","publication_identifier":{"issn":["09607412"]}},{"author":[{"full_name":"Landberg, Katarina","first_name":"Katarina","last_name":"Landberg"},{"first_name":"Eric","last_name":"Pederson","full_name":"Pederson, Eric"},{"last_name":"Viaene","first_name":"Tom","full_name":"Viaene, Tom"},{"full_name":"Bozorg, Behruz","last_name":"Bozorg","first_name":"Behruz"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"},{"full_name":"Jönsson, Henrik","last_name":"Jönsson","first_name":"Henrik"},{"last_name":"Thelander","first_name":"Mattias","full_name":"Thelander, Mattias"},{"full_name":"Sundberg, Eva","last_name":"Sundberg","first_name":"Eva"}],"issue":"3","citation":{"ama":"Landberg K, Pederson E, Viaene T, et al. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. <i>Plant Physiology</i>. 2013;162(3):1406-1419. doi:<a href=\"https://doi.org/10.1104/pp.113.214023\">10.1104/pp.113.214023</a>","apa":"Landberg, K., Pederson, E., Viaene, T., Bozorg, B., Friml, J., Jönsson, H., … Sundberg, E. (2013). The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.113.214023\">https://doi.org/10.1104/pp.113.214023</a>","ieee":"K. Landberg <i>et al.</i>, “The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain,” <i>Plant Physiology</i>, vol. 162, no. 3. American Society of Plant Biologists, pp. 1406–1419, 2013.","mla":"Landberg, Katarina, et al. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” <i>Plant Physiology</i>, vol. 162, no. 3, American Society of Plant Biologists, 2013, pp. 1406–19, doi:<a href=\"https://doi.org/10.1104/pp.113.214023\">10.1104/pp.113.214023</a>.","short":"K. Landberg, E. Pederson, T. Viaene, B. Bozorg, J. Friml, H. Jönsson, M. Thelander, E. Sundberg, Plant Physiology 162 (2013) 1406–1419.","chicago":"Landberg, Katarina, Eric Pederson, Tom Viaene, Behruz Bozorg, Jiří Friml, Henrik Jönsson, Mattias Thelander, and Eva Sundberg. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1104/pp.113.214023\">https://doi.org/10.1104/pp.113.214023</a>.","ista":"Landberg K, Pederson E, Viaene T, Bozorg B, Friml J, Jönsson H, Thelander M, Sundberg E. 2013. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. Plant Physiology. 162(3), 1406–1419."},"month":"07","oa_version":"Submitted Version","publication_status":"published","year":"2013","date_published":"2013-07-03T00:00:00Z","quality_controlled":"1","intvolume":"       162","_id":"2808","title":"The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain","oa":1,"department":[{"_id":"JiFr"}],"publist_id":"4079","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707547/"}],"volume":162,"external_id":{"pmid":["23669745"],"isi":["000321325700015"]},"doi":"10.1104/pp.113.214023","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"In order to establish a reference for analysis of the function of auxin and the auxin biosynthesis regulators SHORT INTERNODE/ STYLISH (SHI/STY) during Physcomitrella patens reproductive development, we have described male (antheridial) and female (archegonial) development in detail, including temporal and positional information of organ initiation. This has allowed us to define discrete stages of organ morphogenesis and to show that reproductive organ development in P. patens is highly organized and that organ phyllotaxis differs between vegetative and reproductive development. Using the PpSHI1 and PpSHI2 reporter and knockout lines, the auxin reporters GmGH3pro:GUS and PpPINApro:GFP-GUS, and the auxin-conjugating transgene PpSHI2pro:IAAL, we could show that the PpSHI genes, and by inference also auxin, play important roles for reproductive organ development in moss. The PpSHI genes are required for the apical opening of the reproductive organs, the final differentiation of the egg cell, and the progression of canal cells into a cell death program. The apical cells of the archegonium, the canal cells, and the egg cell are also sites of auxin responsiveness and are affected by reduced levels of active auxin, suggesting that auxin mediates PpSHI function in the reproductive organs."}],"article_processing_charge":"No","scopus_import":"1","language":[{"iso":"eng"}],"date_created":"2018-12-11T11:59:42Z","status":"public","day":"03","type":"journal_article","date_updated":"2025-09-29T14:06:13Z","isi":1,"publication":"Plant Physiology","publisher":"American Society of Plant Biologists","page":"1406 - 1419","pmid":1},{"oa":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634696/","open_access":"1"}],"department":[{"_id":"JiFr"}],"publist_id":"3980","intvolume":"        25","quality_controlled":"1","_id":"2821","title":"A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis","date_published":"2013-04-24T00:00:00Z","oa_version":"Submitted Version","publication_status":"published","year":"2013","author":[{"full_name":"Remy, Estelle","last_name":"Remy","first_name":"Estelle"},{"last_name":"Cabrito","first_name":"Tânia","full_name":"Cabrito, Tânia"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel","last_name":"Baster","first_name":"Pawel"},{"last_name":"Batista","first_name":"Rita","full_name":"Batista, Rita"},{"full_name":"Teixeira, Miguel","first_name":"Miguel","last_name":"Teixeira"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"},{"last_name":"Sá Correia","first_name":"Isabel","full_name":"Sá Correia, Isabel"},{"first_name":"Paula","last_name":"Duque","full_name":"Duque, Paula"}],"citation":{"short":"E. Remy, T. Cabrito, P. Baster, R. Batista, M. Teixeira, J. Friml, I. Sá Correia, P. Duque, Plant Cell 25 (2013) 901–926.","chicago":"Remy, Estelle, Tânia Cabrito, Pawel Baster, Rita Batista, Miguel Teixeira, Jiří Friml, Isabel Sá Correia, and Paula Duque. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1105/tpc.113.110353\">https://doi.org/10.1105/tpc.113.110353</a>.","ista":"Remy E, Cabrito T, Baster P, Batista R, Teixeira M, Friml J, Sá Correia I, Duque P. 2013. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell. 25(3), 901–926.","ama":"Remy E, Cabrito T, Baster P, et al. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. <i>Plant Cell</i>. 2013;25(3):901-926. doi:<a href=\"https://doi.org/10.1105/tpc.113.110353\">10.1105/tpc.113.110353</a>","ieee":"E. Remy <i>et al.</i>, “A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis,” <i>Plant Cell</i>, vol. 25, no. 3. American Society of Plant Biologists, pp. 901–926, 2013.","apa":"Remy, E., Cabrito, T., Baster, P., Batista, R., Teixeira, M., Friml, J., … Duque, P. (2013). A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.113.110353\">https://doi.org/10.1105/tpc.113.110353</a>","mla":"Remy, Estelle, et al. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” <i>Plant Cell</i>, vol. 25, no. 3, American Society of Plant Biologists, 2013, pp. 901–26, doi:<a href=\"https://doi.org/10.1105/tpc.113.110353\">10.1105/tpc.113.110353</a>."},"issue":"3","month":"04","publication":"Plant Cell","publisher":"American Society of Plant Biologists","isi":1,"pmid":1,"page":"901 - 926","date_created":"2018-12-11T11:59:46Z","status":"public","date_updated":"2025-09-29T13:57:58Z","day":"24","type":"journal_article","abstract":[{"lang":"eng","text":"Many key aspects of plant development are regulated by the polarized transport of the phytohormone auxin. Cellular auxin efflux, the rate-limiting step in this process, has been shown to rely on the coordinated action of PIN-formed (PIN) and B-type ATP binding cassette (ABCB) carriers. Here, we report that polar auxin transport in the Arabidopsis thaliana root also requires the action of a Major Facilitator Superfamily (MFS) transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1). Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf stomatal guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 transporter regulates various root auxin-related processes, while the ZIFL1.3 isoform mediates drought tolerance by regulating stomatal closure. Auxin transport and immunolocalization assays demonstrate that ZIFL1.1 indirectly modulates cellular auxin efflux during shootward auxin transport at the root tip, likely by regulating plasma membrane PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1 and ZIFL1.3 share H+-coupled K+ transport activity. Thus, by determining the subcellular and tissue distribution of two isoforms, alternative splicing dictates a dual function for the ZIFL1 transporter. We propose that this MFS carrier regulates stomatal movements and polar auxin transport by modulating potassium and proton fluxes in Arabidopsis cells."}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"scopus_import":"1","volume":25,"external_id":{"isi":["000318157100012"],"pmid":["23524662"]},"doi":"10.1105/tpc.113.110353"},{"oa":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651428/"}],"department":[{"_id":"JiFr"}],"publist_id":"3972","intvolume":"       110","quality_controlled":"1","_id":"2827","title":"Salicylic acid interferes with clathrin-mediated endocytic protein trafficking","date_published":"2013-05-07T00:00:00Z","publication_status":"published","oa_version":"Submitted Version","year":"2013","corr_author":"1","author":[{"full_name":"Du, Yunlong","last_name":"Du","first_name":"Yunlong"},{"last_name":"Tejos","first_name":"Ricardo","full_name":"Tejos, Ricardo"},{"full_name":"Beck, Martina","first_name":"Martina","last_name":"Beck"},{"first_name":"Ellie","last_name":"Himschoot","full_name":"Himschoot, Ellie"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","first_name":"Hongjiang","last_name":"Li","full_name":"Li, Hongjiang"},{"last_name":"Robatzek","first_name":"Silke","full_name":"Robatzek, Silke"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"}],"citation":{"ista":"Du Y, Tejos R, Beck M, Himschoot E, Li H, Robatzek S, Vanneste S, Friml J. 2013. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. 110(19), 7946–7951.","short":"Y. Du, R. Tejos, M. Beck, E. Himschoot, H. Li, S. Robatzek, S. Vanneste, J. Friml, PNAS 110 (2013) 7946–7951.","chicago":"Du, Yunlong, Ricardo Tejos, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiří Friml. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>. National Academy of Sciences, 2013. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>.","ama":"Du Y, Tejos R, Beck M, et al. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. 2013;110(19):7946-7951. doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>","mla":"Du, Yunlong, et al. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>, vol. 110, no. 19, National Academy of Sciences, 2013, pp. 7946–51, doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>.","ieee":"Y. Du <i>et al.</i>, “Salicylic acid interferes with clathrin-mediated endocytic protein trafficking,” <i>PNAS</i>, vol. 110, no. 19. National Academy of Sciences, pp. 7946–7951, 2013.","apa":"Du, Y., Tejos, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., … Friml, J. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>"},"issue":"19","month":"05","publication":"PNAS","publisher":"National Academy of Sciences","isi":1,"pmid":1,"page":"7946 - 7951","date_created":"2018-12-11T11:59:48Z","status":"public","date_updated":"2025-09-29T13:52:38Z","day":"07","type":"journal_article","abstract":[{"text":"Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.","lang":"eng"}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"scopus_import":"1","volume":110,"project":[{"_id":"2574781E-B435-11E9-9278-68D0E5697425","name":"Koerber Prize"}],"external_id":{"pmid":["23613581"],"isi":["000319327700089"]},"doi":"10.1073/pnas.1220205110"},{"volume":9,"pubrep_id":"411","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"doi":"10.1371/journal.pgen.1003540","external_id":{"isi":["000320030000043"]},"article_processing_charge":"No","abstract":[{"text":"PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"article_number":"e1003540","scopus_import":"1","status":"public","date_created":"2018-12-11T11:59:50Z","date_updated":"2025-09-29T13:50:00Z","type":"journal_article","day":"05","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ec_funded":1,"publisher":"Public Library of Science","publication":"PLoS Genetics","file_date_updated":"2020-07-14T12:45:50Z","isi":1,"author":[{"first_name":"Hirokazu","last_name":"Tanaka","full_name":"Tanaka, Hirokazu"},{"full_name":"Kitakura, Saeko","first_name":"Saeko","last_name":"Kitakura"},{"full_name":"Rakusová, Hana","last_name":"Rakusová","first_name":"Hana"},{"first_name":"Tomohiro","last_name":"Uemura","full_name":"Uemura, Tomohiro"},{"full_name":"Feraru, Mugurel","first_name":"Mugurel","last_name":"Feraru"},{"first_name":"Riet","last_name":"De Rycke","full_name":"De Rycke, Riet"},{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"},{"full_name":"Kakimoto, Tatsuo","last_name":"Kakimoto","first_name":"Tatsuo"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"}],"month":"05","issue":"5","citation":{"apa":"Tanaka, H., Kitakura, S., Rakusová, H., Uemura, T., Feraru, M., De Rycke, R., … Friml, J. (2013). Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1003540\">https://doi.org/10.1371/journal.pgen.1003540</a>","ieee":"H. Tanaka <i>et al.</i>, “Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana,” <i>PLoS Genetics</i>, vol. 9, no. 5. Public Library of Science, 2013.","mla":"Tanaka, Hirokazu, et al. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” <i>PLoS Genetics</i>, vol. 9, no. 5, e1003540, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003540\">10.1371/journal.pgen.1003540</a>.","ama":"Tanaka H, Kitakura S, Rakusová H, et al. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. <i>PLoS Genetics</i>. 2013;9(5). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003540\">10.1371/journal.pgen.1003540</a>","chicago":"Tanaka, Hirokazu, Saeko Kitakura, Hana Rakusová, Tomohiro Uemura, Mugurel Feraru, Riet De Rycke, Stéphanie Robert, Tatsuo Kakimoto, and Jiří Friml. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” <i>PLoS Genetics</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pgen.1003540\">https://doi.org/10.1371/journal.pgen.1003540</a>.","short":"H. Tanaka, S. Kitakura, H. Rakusová, T. Uemura, M. Feraru, R. De Rycke, S. Robert, T. Kakimoto, J. Friml, PLoS Genetics 9 (2013).","ista":"Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, Robert S, Kakimoto T, Friml J. 2013. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. PLoS Genetics. 9(5), e1003540."},"date_published":"2013-05-05T00:00:00Z","year":"2013","oa_version":"Published Version","publication_status":"published","corr_author":"1","intvolume":"         9","quality_controlled":"1","title":"Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana","_id":"2832","file":[{"relation":"main_file","content_type":"application/pdf","file_name":"IST-2016-411-v1+1_journal.pgen.1003540.pdf","checksum":"050237d6c53e8d1601b26808ee1dd6d8","file_id":"4957","date_created":"2018-12-12T10:12:39Z","file_size":3813091,"creator":"system","date_updated":"2020-07-14T12:45:50Z","access_level":"open_access"}],"oa":1,"has_accepted_license":"1","ddc":["570"],"publist_id":"3967","department":[{"_id":"JiFr"}]},{"isi":1,"publisher":"American Society of Plant Biologists","publication":"Plant Physiology","page":"965 - 976","pmid":1,"date_created":"2018-12-11T11:59:51Z","status":"public","type":"journal_article","day":"01","date_updated":"2025-09-29T13:48:15Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"No","abstract":[{"lang":"eng","text":"The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane."}],"scopus_import":"1","language":[{"iso":"eng"}],"volume":162,"doi":"10.1104/pp.113.217018","external_id":{"isi":["000319819900034"],"pmid":["23580592"]},"oa":1,"publist_id":"3964","department":[{"_id":"JiFr"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668084/","open_access":"1"}],"quality_controlled":"1","intvolume":"       162","title":"Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis","_id":"2835","year":"2013","oa_version":"Submitted Version","publication_status":"published","date_published":"2013-06-01T00:00:00Z","author":[{"first_name":"Hong","last_name":"Yu","full_name":"Yu, Hong"},{"full_name":"Karampelias, Michael","last_name":"Karampelias","first_name":"Michael"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"first_name":"Wendy","last_name":"Peer","full_name":"Peer, Wendy"},{"full_name":"Swarup, Ranjan","first_name":"Ranjan","last_name":"Swarup"},{"full_name":"Ye, Songqing","last_name":"Ye","first_name":"Songqing"},{"last_name":"Ge","first_name":"Lei","full_name":"Ge, Lei"},{"full_name":"Cohen, Jerry","last_name":"Cohen","first_name":"Jerry"},{"full_name":"Murphy, Angus","first_name":"Angus","last_name":"Murphy"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"},{"full_name":"Estelle, Mark","last_name":"Estelle","first_name":"Mark"}],"month":"06","issue":"2","citation":{"chicago":"Yu, Hong, Michael Karampelias, Stéphanie Robert, Wendy Peer, Ranjan Swarup, Songqing Ye, Lei Ge, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1104/pp.113.217018\">https://doi.org/10.1104/pp.113.217018</a>.","short":"H. Yu, M. Karampelias, S. Robert, W. Peer, R. Swarup, S. Ye, L. Ge, J. Cohen, A. Murphy, J. Friml, M. Estelle, Plant Physiology 162 (2013) 965–976.","ista":"Yu H, Karampelias M, Robert S, Peer W, Swarup R, Ye S, Ge L, Cohen J, Murphy A, Friml J, Estelle M. 2013. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. Plant Physiology. 162(2), 965–976.","ama":"Yu H, Karampelias M, Robert S, et al. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. <i>Plant Physiology</i>. 2013;162(2):965-976. doi:<a href=\"https://doi.org/10.1104/pp.113.217018\">10.1104/pp.113.217018</a>","ieee":"H. Yu <i>et al.</i>, “Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis,” <i>Plant Physiology</i>, vol. 162, no. 2. American Society of Plant Biologists, pp. 965–976, 2013.","apa":"Yu, H., Karampelias, M., Robert, S., Peer, W., Swarup, R., Ye, S., … Estelle, M. (2013). Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.113.217018\">https://doi.org/10.1104/pp.113.217018</a>","mla":"Yu, Hong, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” <i>Plant Physiology</i>, vol. 162, no. 2, American Society of Plant Biologists, 2013, pp. 965–76, doi:<a href=\"https://doi.org/10.1104/pp.113.217018\">10.1104/pp.113.217018</a>."}},{"page":"817 - 822","publication":"Current Biology","publisher":"Cell Press","ec_funded":1,"isi":1,"date_updated":"2025-09-29T13:43:30Z","day":"06","type":"journal_article","status":"public","date_created":"2018-12-11T11:59:53Z","language":[{"iso":"eng"}],"scopus_import":"1","abstract":[{"lang":"eng","text":"As soon as a seed germinates, plant growth relates to gravity to ensure that the root penetrates the soil and the shoot expands aerially. Whereas mechanisms of positive and negative orthogravitropism of primary roots and shoots are relatively well understood [1-3], lateral organs often show more complex growth behavior [4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show a defined gravitropic set-point angle (GSA) that allows radial expansion of the root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture, it so far remains completely unknown how lateral organs partially suppress positive orthogravitropism. Here we show that the phytohormone auxin steers GSA formation and limits positive orthogravitropism in LR. Low and high auxin levels/signaling lead to radial or axial root systems, respectively. At a cellular level, it is the auxin transport-dependent regulation of asymmetric growth in the elongation zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7 and transient PIN3 expression limit auxin redistribution in young LR columella cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin fluxes in the tip of LRs, induces transient, differential growth responses in the elongation zone and, consequently, controls root architecture."}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"}],"external_id":{"isi":["000318750900035"]},"doi":"10.1016/j.cub.2013.03.064","volume":23,"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"publist_id":"3950","_id":"2844","title":"An auxin transport mechanism restricts positive orthogravitropism in lateral roots","intvolume":"        23","quality_controlled":"1","date_published":"2013-05-06T00:00:00Z","publication_status":"published","oa_version":"None","year":"2013","issue":"9","citation":{"chicago":"Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>.","short":"M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková, A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.","ista":"Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 23(9), 817–822.","ama":"Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. 2013;23(9):817-822. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>","ieee":"M. Rosquete <i>et al.</i>, “An auxin transport mechanism restricts positive orthogravitropism in lateral roots,” <i>Current Biology</i>, vol. 23, no. 9. Cell Press, pp. 817–822, 2013.","apa":"Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková, E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>","mla":"Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>, vol. 23, no. 9, Cell Press, 2013, pp. 817–22, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>."},"month":"05","author":[{"last_name":"Rosquete","first_name":"Michel","full_name":"Rosquete, Michel"},{"full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247"},{"first_name":"Peter","last_name":"Marhavy","full_name":"Marhavy, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741"},{"first_name":"Elke","last_name":"Barbez","full_name":"Barbez, Elke"},{"full_name":"Stelzer, Ernst","first_name":"Ernst","last_name":"Stelzer"},{"first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Maizel","first_name":"Alexis","full_name":"Maizel, Alexis"},{"full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"}]},{"status":"public","date_created":"2018-12-11T12:00:07Z","date_updated":"2025-09-29T13:34:38Z","type":"journal_article","day":"26","publisher":"National Academy of Sciences","publication":"PNAS","isi":1,"pmid":1,"page":"3627 - 3632","volume":110,"doi":"10.1073/pnas.1300107110","external_id":{"pmid":["23391733"],"isi":["000315841900083"]},"article_processing_charge":"No","abstract":[{"text":"Gravitropic bending of plant organs is mediated by an asymmetric signaling of the plant hormone auxin between the upper and lower side of the respective organ. Here, we show that also another plant hormone, gibberellic acid (GA), shows asymmetric action during gravitropic responses. Immunodetection using an antibody against GA and monitoring GA signaling output by downstream degradation of DELLA proteins revealed an asymmetric GA distribution and response with the maximum at the lower side of gravistimulated roots. Genetic or pharmacological manipulation of GA levels or response affects gravity-mediated auxin redistribution and root bending response. The higher GA levels at the lower side of the root correlate with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane. The observed increase in PIN2 stability is caused by a specific GA effect on trafficking of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin A-sensitive endosomes. Our results suggest that asymmetric auxin distribution instructive for gravity-induced differential growth is consolidated by the asymmetric action of GA that stabilizes the PIN-dependent auxin stream along the lower side of gravistimulated roots.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"scopus_import":"1","intvolume":"       110","quality_controlled":"1","title":"Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism","_id":"2882","oa":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587205/","open_access":"1"}],"publist_id":"3879","department":[{"_id":"JiFr"}],"author":[{"full_name":"Löfke, Christian","last_name":"Löfke","first_name":"Christian"},{"first_name":"Marta","last_name":"Zwiewka","full_name":"Zwiewka, Marta"},{"full_name":"Heilmann, Ingo","first_name":"Ingo","last_name":"Heilmann"},{"first_name":"Marc","last_name":"Van Montagu","full_name":"Van Montagu, Marc"},{"full_name":"Teichmann, Thomas","last_name":"Teichmann","first_name":"Thomas"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"month":"02","issue":"9","citation":{"apa":"Löfke, C., Zwiewka, M., Heilmann, I., Van Montagu, M., Teichmann, T., &#38; Friml, J. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1300107110\">https://doi.org/10.1073/pnas.1300107110</a>","ieee":"C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, and J. Friml, “Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism,” <i>PNAS</i>, vol. 110, no. 9. National Academy of Sciences, pp. 3627–3632, 2013.","mla":"Löfke, Christian, et al. “Asymmetric Gibberellin Signaling Regulates Vacuolar Trafficking of PIN Auxin Transporters during Root Gravitropism.” <i>PNAS</i>, vol. 110, no. 9, National Academy of Sciences, 2013, pp. 3627–32, doi:<a href=\"https://doi.org/10.1073/pnas.1300107110\">10.1073/pnas.1300107110</a>.","ama":"Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. <i>PNAS</i>. 2013;110(9):3627-3632. doi:<a href=\"https://doi.org/10.1073/pnas.1300107110\">10.1073/pnas.1300107110</a>","chicago":"Löfke, Christian, Marta Zwiewka, Ingo Heilmann, Marc Van Montagu, Thomas Teichmann, and Jiří Friml. “Asymmetric Gibberellin Signaling Regulates Vacuolar Trafficking of PIN Auxin Transporters during Root Gravitropism.” <i>PNAS</i>. National Academy of Sciences, 2013. <a href=\"https://doi.org/10.1073/pnas.1300107110\">https://doi.org/10.1073/pnas.1300107110</a>.","short":"C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, J. Friml, PNAS 110 (2013) 3627–3632.","ista":"Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. 2013. Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS. 110(9), 3627–3632."},"date_published":"2013-02-26T00:00:00Z","year":"2013","publication_status":"published","oa_version":"Submitted Version"},{"date_published":"2013-01-01T00:00:00Z","publication_status":"published","oa_version":"Submitted Version","year":"2013","citation":{"ista":"Wang B, Bailly A, Zwiewk M, Henrichs S, Azzarello E, Mancuso S, Maeshima M, Friml J, Schulz A, Geisler M. 2013. Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. 25(1), 202–214.","short":"B. Wang, A. Bailly, M. Zwiewk, S. Henrichs, E. Azzarello, S. Mancuso, M. Maeshima, J. Friml, A. Schulz, M. Geisler, Plant Cell 25 (2013) 202–214.","chicago":"Wang, Bangjun, Aurélien Bailly, Marta Zwiewk, Sina Henrichs, Elisa Azzarello, Stefano Mancuso, Masayoshi Maeshima, Jiří Friml, Alexander Schulz, and Markus Geisler. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane.” <i>Plant Cell</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1105/tpc.112.105999\">https://doi.org/10.1105/tpc.112.105999</a>.","ama":"Wang B, Bailly A, Zwiewk M, et al. Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. <i>Plant Cell</i>. 2013;25(1):202-214. doi:<a href=\"https://doi.org/10.1105/tpc.112.105999\">10.1105/tpc.112.105999</a>","mla":"Wang, Bangjun, et al. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane.” <i>Plant Cell</i>, vol. 25, no. 1, American Society of Plant Biologists, 2013, pp. 202–14, doi:<a href=\"https://doi.org/10.1105/tpc.112.105999\">10.1105/tpc.112.105999</a>.","ieee":"B. Wang <i>et al.</i>, “Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane,” <i>Plant Cell</i>, vol. 25, no. 1. American Society of Plant Biologists, pp. 202–214, 2013.","apa":"Wang, B., Bailly, A., Zwiewk, M., Henrichs, S., Azzarello, E., Mancuso, S., … Geisler, M. (2013). Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.112.105999\">https://doi.org/10.1105/tpc.112.105999</a>"},"issue":"1","month":"01","author":[{"first_name":"Bangjun","last_name":"Wang","full_name":"Wang, Bangjun"},{"full_name":"Bailly, Aurélien","first_name":"Aurélien","last_name":"Bailly"},{"last_name":"Zwiewk","first_name":"Marta","full_name":"Zwiewk, Marta"},{"full_name":"Henrichs, Sina","first_name":"Sina","last_name":"Henrichs"},{"first_name":"Elisa","last_name":"Azzarello","full_name":"Azzarello, Elisa"},{"full_name":"Mancuso, Stefano","first_name":"Stefano","last_name":"Mancuso"},{"first_name":"Masayoshi","last_name":"Maeshima","full_name":"Maeshima, Masayoshi"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí"},{"first_name":"Alexander","last_name":"Schulz","full_name":"Schulz, Alexander"},{"last_name":"Geisler","first_name":"Markus","full_name":"Geisler, Markus"}],"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584535/"}],"department":[{"_id":"JiFr"}],"publist_id":"3878","oa":1,"_id":"2883","title":"Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane","intvolume":"        25","quality_controlled":"1","language":[{"iso":"eng"}],"scopus_import":"1","abstract":[{"lang":"eng","text":"Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm."}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"pmid":["23321285"],"isi":["000315572400017"]},"doi":"10.1105/tpc.112.105999","volume":25,"pmid":1,"acknowledgement":"We would thank Vincent Vincenzetti and Laurence Charrier for excellent technical assistance, A. von Arnim for the donation of BRET vectors, E. Spalding for TWD1-CFP, TWD1-CFP/29-1-GFP/ER-YFP, and ABCB4-GFP lines, M. Palmgren for discussion and support, and E. Martinoia for TT12 cDNA, support, and mentorship. Imaging data were partially collected at the Center for Advanced Bioimaging, University of Copenhagen, Denmark. This work was supported by grants from the Novartis Foundation (to M.G.), from the Danish Research School for Biotechnology (to M.G. and A.S.), from the Forschungskredit of the University of Zurich (to A.B.), from the Pool de Recherche of the University of Fribourg (to M.G.), and from the Swiss National Funds (to M.G.). M.G. dedicates this work to his father, who passed away during the resubmission process.","page":"202 - 214","publication":"Plant Cell","publisher":"American Society of Plant Biologists","isi":1,"date_updated":"2025-09-29T13:34:07Z","day":"01","type":"journal_article","date_created":"2018-12-11T12:00:08Z","status":"public"},{"article_processing_charge":"No","abstract":[{"lang":"eng","text":"The distribution of the phytohormone auxin regulates many aspects of plant development including growth response to gravity. Gravitropic root curvature involves coordinated and asymmetric cell elongation between the lower and upper side of the root, mediated by differential cellular auxin levels. The asymmetry in the auxin distribution is established and maintained by a spatio-temporal regulation of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights into the complex regulation of PIN abundance and activity during root gravitropism. We show that PIN2 turnover is differentially regulated on the upper and lower side of gravistimulated roots by distinct but partially overlapping auxin feedback mechanisms. In addition to regulating transcription and clathrin-mediated internalization, auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar targeting and degradation. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. These feedback mechanisms are functionally important during gravitropic response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating asymmetric growth."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"scopus_import":"1","volume":32,"doi":"10.1038/emboj.2012.310","external_id":{"pmid":["23211744"],"isi":["000316464500009"]},"publisher":"Wiley-Blackwell","publication":"EMBO Journal","isi":1,"pmid":1,"page":"260 - 274","date_created":"2018-12-11T12:00:20Z","status":"public","date_updated":"2025-09-29T13:28:19Z","type":"journal_article","day":"23","date_published":"2013-01-23T00:00:00Z","year":"2013","oa_version":"Submitted Version","publication_status":"published","corr_author":"1","author":[{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel","first_name":"Pawel","last_name":"Baster"},{"full_name":"Robert, Stéphanie","last_name":"Robert","first_name":"Stéphanie"},{"full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen","last_name":"Kleine Vehn"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"last_name":"Kania","first_name":"Urszula","full_name":"Kania, Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Wim","last_name":"Grunewald","full_name":"Grunewald, Wim"},{"full_name":"De Rybel, Bert","last_name":"De Rybel","first_name":"Bert"},{"last_name":"Beeckman","first_name":"Tom","full_name":"Beeckman, Tom"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"}],"month":"01","issue":"2","citation":{"ista":"Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274.","chicago":"Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste, Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during Root Gravitropism.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2012.310\">https://doi.org/10.1038/emboj.2012.310</a>.","short":"P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald, B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.","mla":"Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during Root Gravitropism.” <i>EMBO Journal</i>, vol. 32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:<a href=\"https://doi.org/10.1038/emboj.2012.310\">10.1038/emboj.2012.310</a>.","apa":"Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald, W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2012.310\">https://doi.org/10.1038/emboj.2012.310</a>","ieee":"P. Baster <i>et al.</i>, “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism,” <i>EMBO Journal</i>, vol. 32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.","ama":"Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. <i>EMBO Journal</i>. 2013;32(2):260-274. doi:<a href=\"https://doi.org/10.1038/emboj.2012.310\">10.1038/emboj.2012.310</a>"},"oa":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/"}],"publist_id":"3818","department":[{"_id":"JiFr"}],"intvolume":"        32","quality_controlled":"1","title":"SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism","_id":"2919"},{"intvolume":"         2","quality_controlled":"1","title":"Calcium: The missing link in auxin action","_id":"10895","publication_identifier":{"issn":["2223-7747"]},"file":[{"creator":"dernst","file_size":670188,"date_created":"2022-03-21T12:12:56Z","file_id":"10916","success":1,"access_level":"open_access","date_updated":"2022-03-21T12:12:56Z","content_type":"application/pdf","relation":"main_file","checksum":"fb4ff2e820e344e253c9197544610be6","file_name":"2013_Plants_Vanneste.pdf"}],"oa":1,"has_accepted_license":"1","ddc":["580"],"department":[{"_id":"JiFr"}],"author":[{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří"}],"keyword":["Plant Science","Ecology","Ecology","Evolution","Behavior and Systematics"],"month":"10","issue":"4","citation":{"ista":"Vanneste S, Friml J. 2013. Calcium: The missing link in auxin action. Plants. 2(4), 650–675.","short":"S. Vanneste, J. Friml, Plants 2 (2013) 650–675.","chicago":"Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.” <i>Plants</i>. MDPI, 2013. <a href=\"https://doi.org/10.3390/plants2040650\">https://doi.org/10.3390/plants2040650</a>.","mla":"Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.” <i>Plants</i>, vol. 2, no. 4, MDPI, 2013, pp. 650–75, doi:<a href=\"https://doi.org/10.3390/plants2040650\">10.3390/plants2040650</a>.","ieee":"S. Vanneste and J. Friml, “Calcium: The missing link in auxin action,” <i>Plants</i>, vol. 2, no. 4. MDPI, pp. 650–675, 2013.","apa":"Vanneste, S., &#38; Friml, J. (2013). Calcium: The missing link in auxin action. <i>Plants</i>. MDPI. <a href=\"https://doi.org/10.3390/plants2040650\">https://doi.org/10.3390/plants2040650</a>","ama":"Vanneste S, Friml J. Calcium: The missing link in auxin action. <i>Plants</i>. 2013;2(4):650-675. doi:<a href=\"https://doi.org/10.3390/plants2040650\">10.3390/plants2040650</a>"},"date_published":"2013-10-21T00:00:00Z","year":"2013","license":"https://creativecommons.org/licenses/by/3.0/","publication_status":"published","oa_version":"Published Version","corr_author":"1","article_type":"original","date_created":"2022-03-21T07:13:49Z","status":"public","date_updated":"2024-10-09T21:01:52Z","type":"journal_article","day":"21","publisher":"MDPI","tmp":{"image":"/images/cc_by.png","short":"CC BY (3.0)","legal_code_url":"https://creativecommons.org/licenses/by/3.0/legalcode","name":"Creative Commons Attribution 3.0 Unported (CC BY 3.0)"},"publication":"Plants","file_date_updated":"2022-03-21T12:12:56Z","pmid":1,"page":"650-675","volume":2,"doi":"10.3390/plants2040650","external_id":{"pmid":["27137397"]},"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Due to their sessile lifestyles, plants need to deal with the limitations and stresses imposed by the changing environment. Plants cope with these by a remarkable developmental flexibility, which is embedded in their strategy to survive. Plants can adjust their size, shape and number of organs, bend according to gravity and light, and regenerate tissues that were damaged, utilizing a coordinating, intercellular signal, the plant hormone, auxin. Another versatile signal is the cation, Ca2+, which is a crucial second messenger for many rapid cellular processes during responses to a wide range of endogenous and environmental signals, such as hormones, light, drought stress and others. Auxin is a good candidate for one of these Ca2+-activating signals. However, the role of auxin-induced Ca2+ signaling is poorly understood. Here, we will provide an overview of possible developmental and physiological roles, as well as mechanisms underlying the interconnection of Ca2+ and auxin signaling. "}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"scopus_import":"1"},{"year":"2013","publication_status":"published","oa_version":"Submitted Version","date_published":"2013-10-01T00:00:00Z","month":"10","issue":"40","citation":{"short":"Y. Boutté, K. Jonsson, H. Mcfarlane, E. Johnson, D. Gendre, R. Swarup, J. Friml, L. Samuels, S. Robert, R. Bhalerao, PNAS 110 (2013) 16259–16264.","chicago":"Boutté, Yohann, Kristoffer Jonsson, Heather Mcfarlane, Errin Johnson, Delphine Gendre, Ranjan Swarup, Jiří Friml, Lacey Samuels, Stéphanie Robert, and Rishikesh Bhalerao. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential Cell Elongation.” <i>PNAS</i>. National Academy of Sciences, 2013. <a href=\"https://doi.org/10.1073/pnas.1309057110\">https://doi.org/10.1073/pnas.1309057110</a>.","ista":"Boutté Y, Jonsson K, Mcfarlane H, Johnson E, Gendre D, Swarup R, Friml J, Samuels L, Robert S, Bhalerao R. 2013. ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. PNAS. 110(40), 16259–16264.","ama":"Boutté Y, Jonsson K, Mcfarlane H, et al. ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. <i>PNAS</i>. 2013;110(40):16259-16264. doi:<a href=\"https://doi.org/10.1073/pnas.1309057110\">10.1073/pnas.1309057110</a>","apa":"Boutté, Y., Jonsson, K., Mcfarlane, H., Johnson, E., Gendre, D., Swarup, R., … Bhalerao, R. (2013). ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1309057110\">https://doi.org/10.1073/pnas.1309057110</a>","ieee":"Y. Boutté <i>et al.</i>, “ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation,” <i>PNAS</i>, vol. 110, no. 40. National Academy of Sciences, pp. 16259–16264, 2013.","mla":"Boutté, Yohann, et al. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential Cell Elongation.” <i>PNAS</i>, vol. 110, no. 40, National Academy of Sciences, 2013, pp. 16259–64, doi:<a href=\"https://doi.org/10.1073/pnas.1309057110\">10.1073/pnas.1309057110</a>."},"author":[{"full_name":"Boutté, Yohann","last_name":"Boutté","first_name":"Yohann"},{"first_name":"Kristoffer","last_name":"Jonsson","full_name":"Jonsson, Kristoffer"},{"first_name":"Heather","last_name":"Mcfarlane","full_name":"Mcfarlane, Heather"},{"full_name":"Johnson, Errin","first_name":"Errin","last_name":"Johnson"},{"last_name":"Gendre","first_name":"Delphine","full_name":"Gendre, Delphine"},{"full_name":"Swarup, Ranjan","last_name":"Swarup","first_name":"Ranjan"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"full_name":"Samuels, Lacey","last_name":"Samuels","first_name":"Lacey"},{"first_name":"Stéphanie","last_name":"Robert","full_name":"Robert, Stéphanie"},{"full_name":"Bhalerao, Rishikesh","last_name":"Bhalerao","first_name":"Rishikesh"}],"publist_id":"4639","department":[{"_id":"JiFr"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791722/","open_access":"1"}],"oa":1,"title":"ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation","_id":"2290","quality_controlled":"1","intvolume":"       110","scopus_import":"1","language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"No","abstract":[{"text":"The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma membrane are controlled by membrane trafficking processes such as secretion, endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory pathway mediates the localization of auxin carriers is not well understood. In this study we have used the differential cell elongation process during apical hook development to elucidate the mechanisms underlying the post-Golgi trafficking of auxin carriers in Arabidopsis. We show that differential cell elongation during apical hook development is defective in Arabidopsis mutant echidna (ech). ECH protein is required for the trans-Golgi network (TGN)-mediated trafficking of the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation only marginally perturbs the trafficking of the highly related auxin influx carrier LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook development. Electron tomography reveals that the trafficking defects in ech mutant are associated with the perturbation of secretory vesicle genesis from the TGN. Our results identify differential mechanisms for the post-Golgi trafficking of de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal how trafficking of auxin influx carriers mediates the control of differential cell elongation in apical hook development.","lang":"eng"}],"doi":"10.1073/pnas.1309057110","external_id":{"isi":["000325105500090"],"pmid":["24043780"]},"volume":110,"page":"16259 - 16264","pmid":1,"isi":1,"publisher":"National Academy of Sciences","publication":"PNAS","type":"journal_article","day":"01","date_updated":"2025-09-29T14:22:33Z","date_created":"2018-12-11T11:56:48Z","status":"public"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"No","abstract":[{"text":"The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.","lang":"eng"}],"scopus_import":"1","language":[{"iso":"eng"}],"volume":200,"doi":"10.1111/nph.12437","external_id":{"isi":["000330955300012"]},"project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"isi":1,"publisher":"Wiley","ec_funded":1,"publication":"New Phytologist","page":"1034 - 1048","acknowledgement":"The authors thank Dr Christian Luschnig (University of Natural Resources and Life Sciences (BOKU), Vienna, Austria) for the anti-PIN2 antibody, Professor Mark Estelle (University of California, San Diego, CA, USA) for tir1-1 mutant seeds and, last but not least, to Dr David Morris for critical reading of the manuscript. We also thank Markéta Pařezová and Jana Stýblová for excellent technical assistance. This work was supported by the Grant Agency of the Czech Republic (P305/11/0797 to E.Z. and 13-40637S to J.F.), the Central European Institute of Technology project CZ.1.05/1.1.00/02.0068 from the European Regional Development Fund and by a European Research Council starting independent research grant ERC-2011-StG-20101109-PSDP (to J.F.).","status":"public","date_created":"2018-12-11T11:57:41Z","type":"journal_article","day":"01","date_updated":"2025-09-29T14:15:18Z","year":"2013","oa_version":"Published Version","publication_status":"published","date_published":"2013-12-01T00:00:00Z","article_type":"original","author":[{"full_name":"Simon, Sibu","last_name":"Simon","first_name":"Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kubeš, Martin","first_name":"Martin","last_name":"Kubeš"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel","first_name":"Pawel","last_name":"Baster"},{"first_name":"Stéphanie","last_name":"Robert","full_name":"Robert, Stéphanie"},{"first_name":"Petre","last_name":"Dobrev","full_name":"Dobrev, Petre"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"}],"month":"12","citation":{"short":"S. Simon, M. Kubeš, P. Baster, S. Robert, P. Dobrev, J. Friml, J. Petrášek, E. Zažímalová, New Phytologist 200 (2013) 1034–1048.","chicago":"Simon, Sibu, Martin Kubeš, Pawel Baster, Stéphanie Robert, Petre Dobrev, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” <i>New Phytologist</i>. Wiley, 2013. <a href=\"https://doi.org/10.1111/nph.12437\">https://doi.org/10.1111/nph.12437</a>.","ista":"Simon S, Kubeš M, Baster P, Robert S, Dobrev P, Friml J, Petrášek J, Zažímalová E. 2013. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 200(4), 1034–1048.","ieee":"S. Simon <i>et al.</i>, “Defining the selectivity of processes along the auxin response chain: A study using auxin analogues,” <i>New Phytologist</i>, vol. 200, no. 4. Wiley, pp. 1034–1048, 2013.","apa":"Simon, S., Kubeš, M., Baster, P., Robert, S., Dobrev, P., Friml, J., … Zažímalová, E. (2013). Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.12437\">https://doi.org/10.1111/nph.12437</a>","mla":"Simon, Sibu, et al. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” <i>New Phytologist</i>, vol. 200, no. 4, Wiley, 2013, pp. 1034–48, doi:<a href=\"https://doi.org/10.1111/nph.12437\">10.1111/nph.12437</a>.","ama":"Simon S, Kubeš M, Baster P, et al. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. <i>New Phytologist</i>. 2013;200(4):1034-1048. doi:<a href=\"https://doi.org/10.1111/nph.12437\">10.1111/nph.12437</a>"},"issue":"4","oa":1,"publist_id":"4460","department":[{"_id":"JiFr"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.12437"}],"quality_controlled":"1","intvolume":"       200","title":"Defining the selectivity of processes along the auxin response chain: A study using auxin analogues","_id":"2443"},{"intvolume":"         8","quality_controlled":"1","_id":"2448","title":"ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip","oa":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091088/","open_access":"1"}],"department":[{"_id":"JiFr"}],"publist_id":"4455","author":[{"first_name":"Estelle","last_name":"Remy","full_name":"Remy, Estelle"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel","first_name":"Pawel","last_name":"Baster"},{"first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Duque","first_name":"Paula","full_name":"Duque, Paula"}],"issue":"10","citation":{"short":"E. Remy, P. Baster, J. Friml, P. Duque, Plant Signaling &#38; Behavior 8 (2013).","chicago":"Remy, Estelle, Pawel Baster, Jiří Friml, and Paula Duque. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis, 2013. <a href=\"https://doi.org/10.4161/psb.25688\">https://doi.org/10.4161/psb.25688</a>.","ista":"Remy E, Baster P, Friml J, Duque P. 2013. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. Plant Signaling &#38; Behavior. 8(10), e25688.","ama":"Remy E, Baster P, Friml J, Duque P. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. <i>Plant Signaling &#38; Behavior</i>. 2013;8(10). doi:<a href=\"https://doi.org/10.4161/psb.25688\">10.4161/psb.25688</a>","apa":"Remy, E., Baster, P., Friml, J., &#38; Duque, P. (2013). ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.4161/psb.25688\">https://doi.org/10.4161/psb.25688</a>","ieee":"E. Remy, P. Baster, J. Friml, and P. Duque, “ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip,” <i>Plant Signaling &#38; Behavior</i>, vol. 8, no. 10. Taylor &#38; Francis, 2013.","mla":"Remy, Estelle, et al. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” <i>Plant Signaling &#38; Behavior</i>, vol. 8, no. 10, e25688, Taylor &#38; Francis, 2013, doi:<a href=\"https://doi.org/10.4161/psb.25688\">10.4161/psb.25688</a>."},"month":"07","date_published":"2013-07-10T00:00:00Z","publication_status":"published","oa_version":"Submitted Version","year":"2013","article_type":"original","date_created":"2018-12-11T11:57:43Z","status":"public","date_updated":"2025-04-15T07:48:02Z","day":"10","type":"journal_article","publication":"Plant Signaling & Behavior","ec_funded":1,"publisher":"Taylor & Francis","pmid":1,"volume":8,"project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"external_id":{"pmid":["23857365"]},"doi":"10.4161/psb.25688","abstract":[{"text":"Cell-to-cell directional flow of the phytohormone auxin is primarily established by polar localization of the PIN auxin transporters, a process tightly regulated at multiple levels by auxin itself. We recently reported that, in the context of strong auxin flows, activity of the vacuolar ZIFL1.1 transporter is required for fine-tuning of polar auxin transport rates in the Arabidopsis root. In particular, ZIFL1.1 function protects plasma-membrane stability of the PIN2 carrier in epidermal root tip cells under conditions normally triggering PIN2 degradation. Here, we show that ZIFL1.1 activity at the root tip also promotes PIN1 plasma-membrane abundance in central cylinder cells, thus supporting the notion that ZIFL1.1 acts as a general positive modulator of polar auxin transport in roots.","lang":"eng"}],"article_processing_charge":"No","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"e25688","language":[{"iso":"eng"}],"scopus_import":"1"},{"department":[{"_id":"JiFr"}],"publist_id":"4454","_id":"2449","title":"Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis","quality_controlled":"1","intvolume":"         6","corr_author":"1","publication_status":"published","oa_version":"None","year":"2013","date_published":"2013-11-01T00:00:00Z","citation":{"ista":"Nodzyński T, Feraru M, Hirsch S, De Rycke R, Nicuales C, Van Leene J, De Jaeger G, Vanneste S, Friml J. 2013. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant. 6(6), 1849–1862.","short":"T. Nodzyński, M. Feraru, S. Hirsch, R. De Rycke, C. Nicuales, J. Van Leene, G. De Jaeger, S. Vanneste, J. Friml, Molecular Plant 6 (2013) 1849–1862.","chicago":"Nodzyński, Tomasz, Murguel Feraru, Sibylle Hirsch, Riet De Rycke, Claudiu Nicuales, Jelle Van Leene, Geert De Jaeger, Steffen Vanneste, and Jiří Friml. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” <i>Molecular Plant</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1093/mp/sst044\">https://doi.org/10.1093/mp/sst044</a>.","ama":"Nodzyński T, Feraru M, Hirsch S, et al. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. <i>Molecular Plant</i>. 2013;6(6):1849-1862. doi:<a href=\"https://doi.org/10.1093/mp/sst044\">10.1093/mp/sst044</a>","mla":"Nodzyński, Tomasz, et al. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” <i>Molecular Plant</i>, vol. 6, no. 6, Cell Press, 2013, pp. 1849–62, doi:<a href=\"https://doi.org/10.1093/mp/sst044\">10.1093/mp/sst044</a>.","apa":"Nodzyński, T., Feraru, M., Hirsch, S., De Rycke, R., Nicuales, C., Van Leene, J., … Friml, J. (2013). Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1093/mp/sst044\">https://doi.org/10.1093/mp/sst044</a>","ieee":"T. Nodzyński <i>et al.</i>, “Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis,” <i>Molecular Plant</i>, vol. 6, no. 6. Cell Press, pp. 1849–1862, 2013."},"issue":"6","month":"11","author":[{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"last_name":"Feraru","first_name":"Murguel","full_name":"Feraru, Murguel"},{"last_name":"Hirsch","first_name":"Sibylle","full_name":"Hirsch, Sibylle"},{"first_name":"Riet","last_name":"De Rycke","full_name":"De Rycke, Riet"},{"first_name":"Claudiu","last_name":"Nicuales","full_name":"Nicuales, Claudiu"},{"full_name":"Van Leene, Jelle","last_name":"Van Leene","first_name":"Jelle"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"page":"1849 - 1862","isi":1,"publication":"Molecular Plant","publisher":"Cell Press","day":"01","type":"journal_article","date_updated":"2025-09-29T14:14:22Z","status":"public","date_created":"2018-12-11T11:57:44Z","scopus_import":"1","language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1–GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting. ","lang":"eng"}],"article_processing_charge":"No","external_id":{"isi":["000327541200010"]},"doi":"10.1093/mp/sst044","volume":6},{"abstract":[{"text":"Background:Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.Methodology/Principal Findings:Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations.Conclusions/Significance:The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.","lang":"eng"}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"e70050","language":[{"iso":"eng"}],"scopus_import":"1","volume":8,"pubrep_id":"413","external_id":{"isi":["000325211000181"]},"doi":"10.1371/journal.pone.0070050","publication":"PLoS One","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Public Library of Science","file_date_updated":"2020-07-14T12:45:41Z","isi":1,"date_created":"2018-12-11T11:57:51Z","status":"public","date_updated":"2025-09-29T14:11:54Z","day":"23","type":"journal_article","date_published":"2013-07-23T00:00:00Z","oa_version":"Published Version","publication_status":"published","year":"2013","author":[{"last_name":"Čovanová","first_name":"Milada","full_name":"Čovanová, Milada"},{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"last_name":"Rychtář","first_name":"Jan","full_name":"Rychtář, Jan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"}],"citation":{"ama":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. <i>PLoS One</i>. 2013;8(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0070050\">10.1371/journal.pone.0070050</a>","mla":"Čovanová, Milada, et al. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” <i>PLoS One</i>, vol. 8, no. 7, e70050, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0070050\">10.1371/journal.pone.0070050</a>.","ieee":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, and E. Zažímalová, “Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells,” <i>PLoS One</i>, vol. 8, no. 7. Public Library of Science, 2013.","apa":"Čovanová, M., Sauer, M., Rychtář, J., Friml, J., Petrášek, J., &#38; Zažímalová, E. (2013). Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0070050\">https://doi.org/10.1371/journal.pone.0070050</a>","ista":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. 2013. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. PLoS One. 8(7), e70050.","chicago":"Čovanová, Milada, Michael Sauer, Jan Rychtář, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” <i>PLoS One</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pone.0070050\">https://doi.org/10.1371/journal.pone.0070050</a>.","short":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, E. Zažímalová, PLoS One 8 (2013)."},"issue":"7","month":"07","oa":1,"ddc":["570"],"has_accepted_license":"1","department":[{"_id":"JiFr"}],"publist_id":"4432","intvolume":"         8","quality_controlled":"1","_id":"2470","title":"Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells","file":[{"access_level":"open_access","date_updated":"2020-07-14T12:45:41Z","creator":"system","file_id":"4681","file_size":2294955,"date_created":"2018-12-12T10:08:21Z","file_name":"IST-2016-413-v1+1_journal.pone.0070050.pdf","checksum":"2d47ef47616ef4de1d517d146548184e","content_type":"application/pdf","relation":"main_file"}]},{"date_updated":"2025-09-29T14:11:25Z","type":"journal_article","day":"29","date_created":"2018-12-11T11:57:52Z","status":"public","ec_funded":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Public Library of Science","publication":"PLoS One","file_date_updated":"2020-07-14T12:45:41Z","isi":1,"pubrep_id":"393","project":[{"call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis","grant_number":"207362"},{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"doi":"10.1371/journal.pone.0070069","external_id":{"isi":["000323369700128"]},"volume":8,"language":[{"iso":"eng"}],"article_number":"e70069","scopus_import":"1","article_processing_charge":"No","abstract":[{"text":"Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development","_id":"2472","file":[{"creator":"system","file_id":"5222","date_created":"2018-12-12T10:16:34Z","file_size":9003465,"access_level":"open_access","date_updated":"2020-07-14T12:45:41Z","content_type":"application/pdf","relation":"main_file","file_name":"IST-2015-393-v1+1_journal.pone.0070069.pdf","checksum":"3be71828b6c2ba9c90eb7056e3f7f57a"}],"intvolume":"         8","quality_controlled":"1","publist_id":"4431","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"oa":1,"has_accepted_license":"1","ddc":["580","570"],"month":"07","citation":{"chicago":"Cazzonelli, Christopher, Marleen Vanstraelen, Sibu Simon, Kuide Yin, Ashley Carron Arthur, Nazia Nisar, Gauri Tarle, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” <i>PLoS One</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pone.0070069\">https://doi.org/10.1371/journal.pone.0070069</a>.","short":"C. Cazzonelli, M. Vanstraelen, S. Simon, K. Yin, A. Carron Arthur, N. Nisar, G. Tarle, A. Cuttriss, I. Searle, E. Benková, U. Mathesius, J. Masle, J. Friml, B. Pogson, PLoS One 8 (2013).","ista":"Cazzonelli C, Vanstraelen M, Simon S, Yin K, Carron Arthur A, Nisar N, Tarle G, Cuttriss A, Searle I, Benková E, Mathesius U, Masle J, Friml J, Pogson B. 2013. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 8(7), e70069.","ama":"Cazzonelli C, Vanstraelen M, Simon S, et al. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. <i>PLoS One</i>. 2013;8(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0070069\">10.1371/journal.pone.0070069</a>","apa":"Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron Arthur, A., Nisar, N., … Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0070069\">https://doi.org/10.1371/journal.pone.0070069</a>","ieee":"C. Cazzonelli <i>et al.</i>, “Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development,” <i>PLoS One</i>, vol. 8, no. 7. Public Library of Science, 2013.","mla":"Cazzonelli, Christopher, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” <i>PLoS One</i>, vol. 8, no. 7, e70069, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0070069\">10.1371/journal.pone.0070069</a>."},"issue":"7","author":[{"full_name":"Cazzonelli, Christopher","first_name":"Christopher","last_name":"Cazzonelli"},{"full_name":"Vanstraelen, Marleen","first_name":"Marleen","last_name":"Vanstraelen"},{"full_name":"Simon, Sibu","last_name":"Simon","first_name":"Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Yin","first_name":"Kuide","full_name":"Yin, Kuide"},{"full_name":"Carron Arthur, Ashley","last_name":"Carron Arthur","first_name":"Ashley"},{"full_name":"Nisar, Nazia","first_name":"Nazia","last_name":"Nisar"},{"full_name":"Tarle, Gauri","last_name":"Tarle","first_name":"Gauri"},{"full_name":"Cuttriss, Abby","first_name":"Abby","last_name":"Cuttriss"},{"full_name":"Searle, Iain","first_name":"Iain","last_name":"Searle"},{"orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva","last_name":"Benková"},{"full_name":"Mathesius, Ulrike","last_name":"Mathesius","first_name":"Ulrike"},{"last_name":"Masle","first_name":"Josette","full_name":"Masle, Josette"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"},{"last_name":"Pogson","first_name":"Barry","full_name":"Pogson, Barry"}],"date_published":"2013-07-29T00:00:00Z","year":"2013","publication_status":"published","oa_version":"Published Version"}]