[{"date_published":"2024-12-01T00:00:00Z","OA_type":"green","has_accepted_license":"1","ddc":["580"],"article_type":"original","fulldoi":"https://doi.org/10.1016/j.tplants.2024.07.008","OA_place":"repository","date_updated":"2026-10-15T08:27:18Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"Lateral root (LR) formation, that is vital for plant development, is one of many auxin-modulated processes, but the underlying regulatory mechanism is not yet fully known. Recently, \r\nGonzález-García et al. discovered the BiAux compound and showed that it is involved in LR development via regulating specific auxin coreceptors.","lang":"eng"}],"file_date_updated":"2025-11-24T15:12:50Z","issue":"12","external_id":{"isi":["001375335200001"],"pmid":["39079770"]},"intvolume":"        29","file":[{"content_type":"application/pdf","date_updated":"2025-11-24T15:12:50Z","creator":"dernst","access_level":"open_access","checksum":"89a83fe25b9ec1aad8e22d134a342c09","relation":"main_file","file_name":"2024_TrendsPlantScience_Wojcikowska_submittedversion.pdf","date_created":"2025-11-24T15:12:50Z","file_size":277636,"success":1,"file_id":"20691"}],"status":"public","_id":"17377","researchdata_availability":"no","oa_version":"Submitted Version","isi":1,"das_tickbox":"0","day":"01","publisher":"Elsevier","citation":{"ista":"Wójcikowska B, Friml J, Mazur E. 2024. BiAux, a newly discovered compound triggering auxin signaling. Trends in Plant Science. 29(12), 1279–1281.","ama":"Wójcikowska B, Friml J, Mazur E. BiAux, a newly discovered compound triggering auxin signaling. <i>Trends in Plant Science</i>. 2024;29(12):1279-1281. doi:<a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">10.1016/j.tplants.2024.07.008</a>","ieee":"B. Wójcikowska, J. Friml, and E. Mazur, “BiAux, a newly discovered compound triggering auxin signaling,” <i>Trends in Plant Science</i>, vol. 29, no. 12. Elsevier, pp. 1279–1281, 2024.","apa":"Wójcikowska, B., Friml, J., &#38; Mazur, E. (2024). BiAux, a newly discovered compound triggering auxin signaling. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">https://doi.org/10.1016/j.tplants.2024.07.008</a>","chicago":"Wójcikowska, Barbara, Jiří Friml, and Ewa Mazur. “BiAux, a Newly Discovered Compound Triggering Auxin Signaling.” <i>Trends in Plant Science</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">https://doi.org/10.1016/j.tplants.2024.07.008</a>.","short":"B. Wójcikowska, J. Friml, E. Mazur, Trends in Plant Science 29 (2024) 1279–1281.","mla":"Wójcikowska, Barbara, et al. “BiAux, a Newly Discovered Compound Triggering Auxin Signaling.” <i>Trends in Plant Science</i>, vol. 29, no. 12, Elsevier, 2024, pp. 1279–81, doi:<a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">10.1016/j.tplants.2024.07.008</a>."},"acknowledgement":"This work was supported by funding from the National Science Centre, Poland, under the OPUS call in the Weave program and the Austrian Science Fund, Austria project (FWF)/OPUS – Peptide receptor complexes for auxin canalization and regeneration in Arabidopsis (grant \r\n2021/43/I/NZ1/01835) to E.M., and grant I 6123-B to J.F.","year":"2024","oa":1,"title":"BiAux, a newly discovered compound triggering auxin signaling","pmid":1,"publication_identifier":{"issn":["1360-1385"]},"type":"journal_article","doi":"10.1016/j.tplants.2024.07.008","corr_author":"1","scopus_import":"1","publication":"Trends in Plant Science","author":[{"first_name":"Barbara","last_name":"Wójcikowska","full_name":"Wójcikowska, Barbara"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"first_name":"Ewa","full_name":"Mazur, Ewa","last_name":"Mazur"}],"article_processing_charge":"No","supplementarymaterial":"no","date_created":"2024-08-04T22:01:22Z","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"quality_controlled":"1","page":"1279-1281","publication_status":"published","volume":29,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"month":"12"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented."}],"date_updated":"2026-10-15T08:09:43Z","alternative_title":["Methods in Molecular Biology"],"date_published":"2024-04-25T00:00:00Z","fulldoi":"https://doi.org/10.1007/978-1-0716-3778-4_21","das_tickbox":"0","day":"25","citation":{"chicago":"Jayasree, Aswathy, Hymavathi Salava, Tomasz Nodzynski, and Thula Sravankumar. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” In <i>Plant Functional Genomics</i>, edited by Fatemeh Maghuly, 2787:305–13. MIMB. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">https://doi.org/10.1007/978-1-0716-3778-4_21</a>.","mla":"Jayasree, Aswathy, et al. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” <i>Plant Functional Genomics</i>, edited by Fatemeh Maghuly, vol. 2787, Springer Nature, 2024, pp. 305–13, doi:<a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">10.1007/978-1-0716-3778-4_21</a>.","short":"A. Jayasree, H. Salava, T. Nodzynski, T. Sravankumar, in:, F. Maghuly (Ed.), Plant Functional Genomics, Springer Nature, 2024, pp. 305–313.","ista":"Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology, vol. 2787, 305–313.","ama":"Jayasree A, Salava H, Nodzynski T, Sravankumar T. Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Maghuly F, ed. <i>Plant Functional Genomics</i>. Vol 2787. MIMB. Springer Nature; 2024:305-313. doi:<a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">10.1007/978-1-0716-3778-4_21</a>","ieee":"A. Jayasree, H. Salava, T. Nodzynski, and T. Sravankumar, “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf,” in <i>Plant Functional Genomics</i>, vol. 2787, F. Maghuly, Ed. Springer Nature, 2024, pp. 305–313.","apa":"Jayasree, A., Salava, H., Nodzynski, T., &#38; Sravankumar, T. (2024). Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In F. Maghuly (Ed.), <i>Plant Functional Genomics</i> (Vol. 2787, pp. 305–313). Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">https://doi.org/10.1007/978-1-0716-3778-4_21</a>"},"year":"2024","acknowledgement":"Special thanks to Dr. Marta Zwiewka for the support. Thanks to the Czech Science Foundation GA 20-20860Y for financial aid and support of A.S.S., respectively. Thanks go to Core Facility Cellular Imaging (CELLIM), and Plant Sciences Core Facility of CEITEC Masaryk University is acknowledged for the technical support.","publisher":"Springer Nature","status":"public","_id":"15361","researchdata_availability":"no","editor":[{"full_name":"Maghuly, Fatemeh","last_name":"Maghuly","first_name":"Fatemeh"}],"oa_version":"None","external_id":{"pmid":["38656499"]},"intvolume":"      2787","publication":"Plant Functional Genomics","author":[{"first_name":"Aswathy","full_name":"Jayasree, Aswathy","last_name":"Jayasree"},{"first_name":"Hymavathi","full_name":"Salava, Hymavathi","last_name":"Salava"},{"first_name":"Tomasz","full_name":"Nodzynski, Tomasz","last_name":"Nodzynski"},{"orcid":"0000-0001-6925-6950","first_name":"Thula","full_name":"Sravankumar, Thula","id":"055b7938-0b72-11ef-94eb-d14136011bb5","last_name":"Sravankumar"}],"scopus_import":"1","pmid":1,"publication_identifier":{"isbn":["9781071637777"],"eissn":["1940-6029"]},"title":"Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf","doi":"10.1007/978-1-0716-3778-4_21","type":"book_chapter","month":"04","page":"305-313","series_title":"MIMB","language":[{"iso":"eng"}],"volume":2787,"department":[{"_id":"JiFr"}],"publication_status":"published","quality_controlled":"1","supplementarymaterial":"no","date_created":"2024-05-05T22:01:04Z","article_processing_charge":"No"},{"quality_controlled":"1","date_created":"2024-10-13T22:01:51Z","supplementarymaterial":"no","article_processing_charge":"No","month":"11","language":[{"iso":"eng"}],"volume":34,"department":[{"_id":"JiFr"}],"publication_status":"published","page":"761-762","doi":"10.1038/s41422-024-01035-x","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["1748-7838"],"issn":["1001-0602"]},"title":"Feeling the danger: Local wound signaling in plants","author":[{"first_name":"Lukas","orcid":"0000-0001-8295-2926","full_name":"Hörmayer, Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"}],"publication":"Cell Research","scopus_import":"1","corr_author":"1","intvolume":"        34","external_id":{"isi":["001326684200001"],"pmid":["39354142"]},"citation":{"ama":"Hörmayer L, Friml J. Feeling the danger: Local wound signaling in plants. <i>Cell Research</i>. 2024;34:761-762. doi:<a href=\"https://doi.org/10.1038/s41422-024-01035-x\">10.1038/s41422-024-01035-x</a>","ista":"Hörmayer L, Friml J. 2024. Feeling the danger: Local wound signaling in plants. Cell Research. 34, 761–762.","apa":"Hörmayer, L., &#38; Friml, J. (2024). Feeling the danger: Local wound signaling in plants. <i>Cell Research</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41422-024-01035-x\">https://doi.org/10.1038/s41422-024-01035-x</a>","ieee":"L. Hörmayer and J. Friml, “Feeling the danger: Local wound signaling in plants,” <i>Cell Research</i>, vol. 34. Springer Nature, pp. 761–762, 2024.","chicago":"Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling in Plants.” <i>Cell Research</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41422-024-01035-x\">https://doi.org/10.1038/s41422-024-01035-x</a>.","short":"L. Hörmayer, J. Friml, Cell Research 34 (2024) 761–762.","mla":"Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling in Plants.” <i>Cell Research</i>, vol. 34, Springer Nature, 2024, pp. 761–62, doi:<a href=\"https://doi.org/10.1038/s41422-024-01035-x\">10.1038/s41422-024-01035-x</a>."},"year":"2024","publisher":"Springer Nature","das_tickbox":"0","day":"01","isi":1,"oa_version":"None","researchdata_availability":"no","_id":"18311","status":"public","fulldoi":"https://doi.org/10.1038/s41422-024-01035-x","article_type":"original","OA_type":"closed access","date_published":"2024-11-01T00:00:00Z","abstract":[{"lang":"eng","text":"Local wound signaling in plants informs the surrounding tissues about an injury and initiates the regeneration process. In a recent paper published in Cell, Yang and colleagues show the involvement of a single Pep family member from tomato in wound signaling and how exogenous application of this regeneration factor enhances transformation efficiency in crops."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-15T08:23:58Z"},{"related_material":{"link":[{"url":"https://ista.ac.at/en/news/beneath-the-surface/","description":"News on ISTA website","relation":"press_release"}],"record":[{"id":"22984","relation":"dissertation_contains","status":"public"}]},"quality_controlled":"1","project":[{"grant_number":"742985","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"article_processing_charge":"Yes","supplementarymaterial":"yes","date_created":"2024-04-02T11:35:58Z","month":"03","publication_status":"published","department":[{"_id":"JiFr"}],"volume":12,"language":[{"iso":"eng"}],"article_number":"91523","type":"journal_article","doi":"10.7554/elife.91523","title":"Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism","oa":1,"pmid":1,"publication_identifier":{"issn":["2050-084X"]},"author":[{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"full_name":"Schmid, Julia","id":"07cf4637-baaf-11ee-9227-e1de57d1d69b","last_name":"Schmid","first_name":"Julia"},{"full_name":"Teplova, Anastasiia","id":"e3736151-106c-11ec-b916-c2558e2762c6","last_name":"Teplova","first_name":"Anastasiia"},{"full_name":"Qi, Linlin","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi","first_name":"Linlin","orcid":"0000-0001-5187-8401"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"eLife","scopus_import":"1","corr_author":"1","intvolume":"        12","file":[{"content_type":"application/pdf","date_updated":"2024-04-03T13:18:00Z","creator":"dernst","checksum":"a73a84d3bf97a6d09d24308ca6dd0a0c","access_level":"open_access","relation":"main_file","file_name":"2024_eLife_Kulich.pdf","file_size":11451904,"date_created":"2024-04-03T13:18:00Z","success":1,"file_id":"15288"}],"external_id":{"pmid":["38441122"]},"dataavailabilitystatement":"All data generated in this study are included as a manuscript supporting files.","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"publisher":"eLife Sciences Publications","acknowledgement":"The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F. This research was also supported by the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) of IST Austria, namely Tereza Bělinová for her help with the imaging. JS was supported by FemTECH fellowship.","citation":{"mla":"Kulich, Ivan, et al. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>, vol. 12, 91523, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>.","short":"I. Kulich, J. Schmid, A. Teplova, L. Qi, J. Friml, ELife 12 (2024).","chicago":"Kulich, Ivan, Julia Schmid, Anastasiia Teplova, Linlin Qi, and Jiří Friml. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>.","ieee":"I. Kulich, J. Schmid, A. Teplova, L. Qi, and J. Friml, “Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism,” <i>eLife</i>, vol. 12. eLife Sciences Publications, 2024.","apa":"Kulich, I., Schmid, J., Teplova, A., Qi, L., &#38; Friml, J. (2024). Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>","ista":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. 2024. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. eLife. 12, 91523.","ama":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>eLife</i>. 2024;12. doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>"},"year":"2024","day":"05","das_tickbox":"1","oa_version":"Published Version","_id":"15257","researchdata_availability":"yes (supplementary data)","status":"public","DOAJ_listed":"1","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.7554/elife.91523","article_type":"original","has_accepted_license":"1","date_published":"2024-03-05T00:00:00Z","ddc":["580"],"file_date_updated":"2024-04-03T13:18:00Z","ec_funded":1,"abstract":[{"lang":"eng","text":"Root gravitropic bending represents a fundamental aspect of terrestrial plant physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths) to the bottom of the central root cap cells. Following gravity perception, intercellular auxin transport is redirected downwards leading to an asymmetric auxin accumulation at the lower root side causing inhibition of cell expansion, ultimately resulting in downwards bending. How gravity-induced statoliths repositioning is translated into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith sedimentation, thus providing a plausible mechanism for auxin flow redirection. In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization on the statolith surface and plasma membrane (PM) domains in close proximity to the statoliths, correlating with their movements. We determined that NGR1 binding to these PM domains is indispensable for NGR1 functionality and relies on cysteine acylation and adjacent polybasic regions as well as on lipid and sterol PM composition. Detailed timing of the early events following graviperception suggested that both NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization. This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells, thus providing an attractive model for rapid redirection of auxin fluxes following gravistimulation."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-15T08:30:04Z"},{"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988"}],"quality_controlled":"1","related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/"}]},"article_processing_charge":"Yes (via OA deal)","date_created":"2024-04-08T12:07:57Z","supplementarymaterial":"yes","month":"05","page":"1333-1344.e4","publication_status":"published","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"language":[{"iso":"eng"}],"volume":59,"title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","oa":1,"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"pmid":1,"type":"journal_article","doi":"10.1016/j.devcel.2024.03.009","publication":"Developmental Cell","author":[{"last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","first_name":"Lukas"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","full_name":"Montesinos López, Juan C","first_name":"Juan C","orcid":"0000-0001-9179-6099"},{"full_name":"Trozzi, N","last_name":"Trozzi","first_name":"N"},{"first_name":"Leonhard","full_name":"Spona, Leonhard","last_name":"Spona","id":"b52391fb-f636-11ee-939c-8a8c47552e8a"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","last_name":"Yoshida","full_name":"Yoshida, Saiko","first_name":"Saiko","orcid":"0000-0001-6111-9353"},{"first_name":"Petra","full_name":"Marhavá, Petra","last_name":"Marhavá","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia","orcid":"0000-0002-5223-3346"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"},{"last_name":"Dagdas","full_name":"Dagdas, Y","first_name":"Y"},{"first_name":"M","full_name":"Majda, M","last_name":"Majda"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"corr_author":"1","scopus_import":"1","issue":"10","external_id":{"isi":["001301584600001"],"pmid":["38579717"]},"intvolume":"        59","file":[{"date_updated":"2024-08-20T11:22:16Z","content_type":"application/pdf","creator":"dernst","checksum":"22b374fb50a40d380b7686c84258d271","access_level":"open_access","relation":"main_file","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","date_created":"2024-08-20T11:22:16Z","file_size":5195262,"success":1,"file_id":"17452"}],"dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"isi":1,"day":"20","das_tickbox":"1","publisher":"Elsevier","citation":{"mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>"},"year":"2024","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","researchdata_availability":"upon request","_id":"15301","status":"public","oa_version":"Published Version","date_published":"2024-05-20T00:00:00Z","has_accepted_license":"1","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.devcel.2024.03.009","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2024-08-20T11:22:16Z","ec_funded":1,"abstract":[{"text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture.","lang":"eng"}],"date_updated":"2026-10-15T08:32:32Z"},{"OA_place":"publisher","has_accepted_license":"1","date_published":"2024-12-02T00:00:00Z","OA_type":"hybrid","ddc":["580"],"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.molp.2024.11.001","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis."}],"file_date_updated":"2024-12-03T11:08:09Z","date_updated":"2026-10-15T08:30:20Z","issue":"12","external_id":{"pmid":["39501563"],"isi":["001373778300001"]},"intvolume":"        17","file":[{"success":1,"file_id":"18612","file_name":"2024_MolecularPlant_Kralova.pdf","date_created":"2024-12-03T11:08:09Z","file_size":3308945,"access_level":"open_access","checksum":"a11feea4b1677df76b632eca04bfc1dd","relation":"main_file","date_updated":"2024-12-03T11:08:09Z","content_type":"application/pdf","creator":"dernst"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"isi":1,"das_tickbox":"0","day":"02","publisher":"Elsevier","citation":{"apa":"Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., … Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>","ieee":"M. Králová <i>et al.</i>, “A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis,” <i>Molecular Plant</i>, vol. 17, no. 12. Elsevier, pp. 1850–1865, 2024.","ista":"Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.","ama":"Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. 2024;17(12):1850-1865. doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>","short":"M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C. Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml, O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.","mla":"Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>, vol. 17, no. 12, Elsevier, 2024, pp. 1850–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>.","chicago":"Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>."},"year":"2024","acknowledgement":"We dedicate this paper to the deceased Petr Galuszka for his inspiration and support of our project. We thank Prof. Peter Hedden for constructive criticism of the manuscript and English editing. No conflict of interest is declared.","status":"public","_id":"18596","researchdata_availability":"no","oa_version":"Published Version","title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis","oa":1,"publication_identifier":{"issn":["1674-2052"]},"pmid":1,"type":"journal_article","doi":"10.1016/j.molp.2024.11.001","publication":"Molecular Plant","author":[{"last_name":"Králová","full_name":"Králová, Michaela","first_name":"Michaela"},{"first_name":"Ivona","last_name":"Kubalová","full_name":"Kubalová, Ivona"},{"first_name":"Jakub","full_name":"Hajný, Jakub","last_name":"Hajný"},{"full_name":"Kubiasova, Karolina","last_name":"Kubiasova","id":"946011F4-3E71-11EA-860B-C7A73DDC885E","first_name":"Karolina","orcid":"0000-0001-5630-9419"},{"first_name":"Karolína","full_name":"Vagaská, Karolína","last_name":"Vagaská"},{"orcid":"0000-0001-9381-3577","first_name":"Zengxiang","full_name":"Ge, Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge"},{"first_name":"Michelle C","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","last_name":"Semerádová","full_name":"Semerádová, Hana","first_name":"Hana"},{"full_name":"Kuchařová, Anna","last_name":"Kuchařová","first_name":"Anna"},{"full_name":"Hönig, Martin","last_name":"Hönig","first_name":"Martin"},{"full_name":"Monzer, Aline","last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline"},{"last_name":"Kovačik","full_name":"Kovačik, Martin","first_name":"Martin"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"last_name":"Ikeda","full_name":"Ikeda, Yoshihisa","first_name":"Yoshihisa"},{"full_name":"Zalabák, David","last_name":"Zalabák","first_name":"David"}],"scopus_import":"1","quality_controlled":"1","article_processing_charge":"Yes (in subscription journal)","date_created":"2024-11-28T11:13:35Z","supplementarymaterial":"yes","month":"12","page":"1850-1865","publication_status":"published","volume":17,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}]},{"month":"10","page":"4668-4698","department":[{"_id":"MaLo"},{"_id":"JiFr"}],"language":[{"iso":"eng"}],"volume":43,"publication_status":"published","quality_controlled":"1","supplementarymaterial":"yes","date_created":"2024-09-15T22:01:42Z","article_processing_charge":"Yes","publication":"EMBO Journal","author":[{"last_name":"Kettel","full_name":"Kettel, Paulina","first_name":"Paulina"},{"full_name":"Marosits, Laura","last_name":"Marosits","first_name":"Laura"},{"first_name":"Elena","last_name":"Spinetti","full_name":"Spinetti, Elena"},{"full_name":"Rechberger, Michael","last_name":"Rechberger","first_name":"Michael"},{"full_name":"Giannini, Caterina","last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","first_name":"Caterina"},{"full_name":"Radler, Philipp","last_name":"Radler","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","first_name":"Philipp","orcid":"0000-0001-9198-2182 "},{"full_name":"Niedermoser, Isabell","last_name":"Niedermoser","first_name":"Isabell"},{"full_name":"Fischer, Irmgard","last_name":"Fischer","first_name":"Irmgard"},{"full_name":"Versteeg, Gijs A.","last_name":"Versteeg","first_name":"Gijs A."},{"orcid":"0000-0001-7309-9724","first_name":"Martin","full_name":"Loose, Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose"},{"first_name":"Roberto","last_name":"Covino","full_name":"Covino, Roberto"},{"first_name":"G. Elif","last_name":"Karagöz","full_name":"Karagöz, G. Elif"}],"scopus_import":"1","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"pmid":1,"oa":1,"title":"Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering","doi":"10.1038/s44318-024-00207-0","type":"journal_article","day":"15","das_tickbox":"1","isi":1,"acknowledgement":"We thank late Thomas Peterbauer at the Max Perutz Labs Biooptics Light Microscopy Facility for his help and support. We are grateful to Kitti Csalyi and Thomas Sauer at Max Perutz Labs Biooptics FACS facility for their help. We thank Grzegorz Scibisz and Sertan Atilla for their support with the expression and purification of mCherry-IRE1α LD-10His. We are grateful to Aleksandra S Anisimova with her help in the generation of stable cell lines and the statistical analyses of the data. We thank Venja Vieweger for her help with the characterization of the WLLI and D123P IRE1 mutants in cells. We are thankful to Monika Kubickova for the help with the AUC experiments. We acknowledge CF BIC of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2023042)) and European Regional Development Fund-Project, UP CIISB“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974). We thank the members of the Karagöz lab for the critical reading and editing of the manuscript. We are thankful to our colleagues Diego Acosta-Alvear, Vladislav Belyy, Jirka Peschek, Yasin Dagdas, Javier Martinez, Sascha Martens and Alwin Köhler for their invaluable input on the manuscript. We are grateful to Life Science Editors, especially Katrina Woolcock for her useful edits and comments on the manuscript. We acknowledge funding from Austrian Science Fund (FWF-SFB F79 and FWF-W 1261) to GEK. PK acknowledges the support of the Max Perutz PhD fellowship. GAV is funded by Stand-Alone grants (P30231-B, P30415-B, P36572), Special Research Grant (SFB grant F79), and Doctoral School grant (DK grant W1261) from the Austrian Science Fund (FWF). ES and RC acknowledge support and funding by the Frankfurt Institute of Advanced Studies, the LOEWE Center for Multiscale Modelling in Life Sciences of the state of Hesse, the Collaborative Research Center 1507 “Membrane-associated Protein Assemblies, Machineries, and Supercomplexes” (Project ID 450648163), and the International Max Planck Research School on Cellular Biophysics (to RC), the Center for Scientific Computing of the Goethe University and the Jülich Supercomputing Centre for computational resources and support.","year":"2024","citation":{"chicago":"Kettel, Paulina, Laura Marosits, Elena Spinetti, Michael Rechberger, Caterina Giannini, Philipp Radler, Isabell Niedermoser, et al. “Disordered Regions in the IRE1α ER Lumenal Domain Mediate Its Stress-Induced Clustering.” <i>EMBO Journal</i>. Embo Press, 2024. <a href=\"https://doi.org/10.1038/s44318-024-00207-0\">https://doi.org/10.1038/s44318-024-00207-0</a>.","mla":"Kettel, Paulina, et al. “Disordered Regions in the IRE1α ER Lumenal Domain Mediate Its Stress-Induced Clustering.” <i>EMBO Journal</i>, vol. 43, no. 20, Embo Press, 2024, pp. 4668–98, doi:<a href=\"https://doi.org/10.1038/s44318-024-00207-0\">10.1038/s44318-024-00207-0</a>.","short":"P. Kettel, L. Marosits, E. Spinetti, M. Rechberger, C. Giannini, P. Radler, I. Niedermoser, I. Fischer, G.A. Versteeg, M. Loose, R. Covino, G.E. Karagöz, EMBO Journal 43 (2024) 4668–4698.","ista":"Kettel P, Marosits L, Spinetti E, Rechberger M, Giannini C, Radler P, Niedermoser I, Fischer I, Versteeg GA, Loose M, Covino R, Karagöz GE. 2024. Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. EMBO Journal. 43(20), 4668–4698.","ama":"Kettel P, Marosits L, Spinetti E, et al. Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. <i>EMBO Journal</i>. 2024;43(20):4668-4698. doi:<a href=\"https://doi.org/10.1038/s44318-024-00207-0\">10.1038/s44318-024-00207-0</a>","apa":"Kettel, P., Marosits, L., Spinetti, E., Rechberger, M., Giannini, C., Radler, P., … Karagöz, G. E. (2024). Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.1038/s44318-024-00207-0\">https://doi.org/10.1038/s44318-024-00207-0</a>","ieee":"P. Kettel <i>et al.</i>, “Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering,” <i>EMBO Journal</i>, vol. 43, no. 20. Embo Press, pp. 4668–4698, 2024."},"publisher":"Embo Press","status":"public","_id":"18073","researchdata_availability":"yes","oa_version":"Published Version","external_id":{"isi":["001306286100002"],"pmid":["39232130"]},"issue":"20","file":[{"file_size":10080854,"date_created":"2025-01-13T08:43:20Z","file_name":"2024_Embo_Kettel.pdf","file_id":"18827","success":1,"creator":"dernst","date_updated":"2025-01-13T08:43:20Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","checksum":"04f4df1a561083f2846676442fc4eb3c"}],"intvolume":"        43","dataavailabilitystatement":"All the raw data in this work has been deposited to Biostudies with the accession number: S-BIAD1023. One can access the data through the link below: https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD1023?key=426953ee-3da4-4cf4-a496-e607ce240aff.  The source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44318-024-00207-0.","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2025-01-13T08:43:20Z","abstract":[{"lang":"eng","text":"Conserved signaling cascades monitor protein-folding homeostasis to ensure proper cellular function. One of the evolutionary conserved key players is IRE1, which maintains endoplasmic reticulum (ER) homeostasis through the unfolded protein response (UPR). Upon accumulation of misfolded proteins in the ER, IRE1 forms clusters on the ER membrane to initiate UPR signaling. What regulates IRE1 cluster formation is not fully understood. Here, we show that the ER lumenal domain (LD) of human IRE1α forms biomolecular condensates in vitro. IRE1α LD condensates were stabilized both by binding to unfolded polypeptides as well as by tethering to model membranes, suggesting their role in assembling IRE1α into signaling-competent stable clusters. Molecular dynamics simulations indicated that weak multivalent interactions drive IRE1α LD clustering. Mutagenesis experiments identified disordered regions in IRE1α LD to control its clustering in vitro and in cells. Importantly, dysregulated clustering of IRE1α mutants led to defects in IRE1α signaling. Our results revealed that disordered regions in IRE1α LD control its clustering and suggest their role as a common strategy in regulating protein assembly on membranes."}],"date_updated":"2026-10-16T10:37:47Z","OA_place":"publisher","ddc":["570"],"date_published":"2024-10-15T00:00:00Z","OA_type":"gold","has_accepted_license":"1","fulldoi":"https://doi.org/10.1038/s44318-024-00207-0","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"article_number":"jcs.261720","type":"journal_article","doi":"10.1242/jcs.261720","oa":1,"title":"Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana","pmid":1,"publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"author":[{"first_name":"Nataliia","orcid":"0000-0002-2198-0509","full_name":"Gnyliukh, Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87","last_name":"Gnyliukh"},{"orcid":"0000-0002-2739-8843","first_name":"Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J"},{"full_name":"Nagel, MK","last_name":"Nagel","first_name":"MK"},{"full_name":"Monzer, Aline","last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline"},{"first_name":"David","last_name":"Babic","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","full_name":"Babic, David"},{"id":"36062FEC-F248-11E8-B48F-1D18A9856A87","last_name":"Hlavata","full_name":"Hlavata, Annamaria","first_name":"Annamaria"},{"first_name":"SS","full_name":"Alotaibi, SS","last_name":"Alotaibi"},{"first_name":"E","last_name":"Isono","full_name":"Isono, E"},{"last_name":"Loose","id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin","first_name":"Martin","orcid":"0000-0001-7309-9724"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"Journal of Cell Science","scopus_import":"1","corr_author":"1","related_material":{"record":[{"status":"public","id":"14591","relation":"earlier_version"}]},"quality_controlled":"1","project":[{"call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","grant_number":"665385"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"}],"article_processing_charge":"Yes (via OA deal)","supplementarymaterial":"yes","date_created":"2024-04-19T09:54:59Z","month":"04","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"MaLo"},{"_id":"JiFr"},{"_id":"CaBe"}],"volume":137,"OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1242/jcs.261720","article_type":"original","has_accepted_license":"1","OA_type":"hybrid","date_published":"2024-04-01T00:00:00Z","ddc":["570"],"ec_funded":1,"file_date_updated":"2025-01-09T08:41:16Z","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development by controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin, in the CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-16T10:38:13Z","intvolume":"       137","file":[{"date_created":"2025-01-09T08:41:16Z","file_size":25845948,"file_name":"2024_JourCellScience_Gnyliukh.pdf","success":1,"file_id":"18792","creator":"dernst","date_updated":"2025-01-09T08:41:16Z","content_type":"application/pdf","access_level":"open_access","checksum":"6dc023f0cc7052ad3cf0a42589d2e30f","relation":"main_file"}],"issue":"8","external_id":{"pmid":["38506228"],"isi":["001266917100005"]},"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"publisher":"The Company of Biologists","year":"2024","citation":{"apa":"Gnyliukh, N., Johnson, A. J., Nagel, M., Monzer, A., Babic, D., Hlavata, A., … Friml, J. (2024). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>","ieee":"N. Gnyliukh <i>et al.</i>, “Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana,” <i>Journal of Cell Science</i>, vol. 137, no. 8. The Company of Biologists, 2024.","ama":"Gnyliukh N, Johnson AJ, Nagel M, et al. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. 2024;137(8). doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>","ista":"Gnyliukh N, Johnson AJ, Nagel M, Monzer A, Babic D, Hlavata A, Alotaibi S, Isono E, Loose M, Friml J. 2024. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. Journal of Cell Science. 137(8), jcs. 261720.","short":"N. Gnyliukh, A.J. Johnson, M. Nagel, A. Monzer, D. Babic, A. Hlavata, S. Alotaibi, E. Isono, M. Loose, J. Friml, Journal of Cell Science 137 (2024).","mla":"Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>, vol. 137, no. 8, jcs. 261720, The Company of Biologists, 2024, doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>.","chicago":"Gnyliukh, Nataliia, Alexander J Johnson, MK Nagel, Aline Monzer, David Babic, Annamaria Hlavata, SS Alotaibi, E Isono, Martin Loose, and Jiří Friml. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>."},"acknowledgement":"Nataliia Gnyliukh was partially funded by the European Union’s Horizon 2020 research and\r\ninnovation program (2018-2020) under the Marie Sklodowska-Curie Grant (agreement no.\r\n665385). Taif University Researchers Supporting Project: TURSP-HC2022/02. and Austrian\r\nScience Fund (FWF): I 6123-B.We thank Prof. Eileen Lafer and Liping Wang for their suggestions regarding the optimisation of protein expression and purification. We thank Prof. Sebastian Y. Bednarek for the useful comments and constructive criticism of the project. We thank Maciek Adamowski for providing genetic material. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Electron microscopy (EMF), Lab Support Facility (LSF) (particularly Dorota Jaworska) and the Bioimaging Facility (BIF).","isi":1,"day":"01","das_tickbox":"0","oa_version":"Published Version","researchdata_availability":"no","_id":"15330","status":"public"},{"quality_controlled":"1","project":[{"grant_number":"I03630","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"article_processing_charge":"Yes","date_created":"2024-05-12T22:01:01Z","supplementarymaterial":"yes","month":"05","publication_status":"published","volume":43,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"MaLo"}],"article_number":"114195","type":"journal_article","doi":"10.1016/j.celrep.2024.114195","title":"SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis","oa":1,"publication_identifier":{"eissn":["2211-1247"]},"pmid":1,"author":[{"orcid":"0000-0001-6463-5257","first_name":"Maciek","full_name":"Adamowski, Maciek","last_name":"Adamowski","id":"45F536D2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","full_name":"Randuch, Marek"},{"first_name":"Ivana","last_name":"Matijevic","id":"83c17ce3-15b2-11ec-abd3-f486545870bd","full_name":"Matijevic, Ivana"},{"first_name":"Madhumitha","orcid":"0000-0002-8600-0671","full_name":"Narasimhan, Madhumitha","last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"Cell Reports","scopus_import":"1","corr_author":"1","intvolume":"        43","file":[{"date_created":"2024-05-13T12:11:22Z","file_size":5698598,"file_name":"2024_CellReports_Adamowski.pdf","file_id":"15387","success":1,"creator":"dernst","date_updated":"2024-05-13T12:11:22Z","content_type":"application/pdf","relation":"main_file","checksum":"a06bb85be4fc765c51554d27ee2da802","access_level":"open_access"}],"issue":"5","external_id":{"pmid":["38717900"],"isi":["001240362800001"]},"dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this work paper is available from the lead contact upon request.","publisher":"Cell Press","year":"2024","acknowledgement":"The authors wish to acknowledge Dr. Daniel van Damme for mRuby3/pDONRP2rP3 and Prof. Qi-Jun Chen for sharing plasmids used for CRISPR-Cas9 mutagenesis. This work was supported by the Austrian Science Fund (FWF): I 3630-B25.","citation":{"apa":"Adamowski, M., Randuch, M., Matijevic, I., Narasimhan, M., &#38; Friml, J. (2024). SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">https://doi.org/10.1016/j.celrep.2024.114195</a>","ieee":"M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, and J. Friml, “SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis,” <i>Cell Reports</i>, vol. 43, no. 5. Cell Press, 2024.","ama":"Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. <i>Cell Reports</i>. 2024;43(5). doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">10.1016/j.celrep.2024.114195</a>","ista":"Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. 2024. SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. Cell Reports. 43(5), 114195.","mla":"Adamowski, Maciek, et al. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors for Clathrin-Mediated Endocytosis.” <i>Cell Reports</i>, vol. 43, no. 5, 114195, Cell Press, 2024, doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">10.1016/j.celrep.2024.114195</a>.","short":"M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, J. Friml, Cell Reports 43 (2024).","chicago":"Adamowski, Maciek, Marek Randuch, Ivana Matijevic, Madhumitha Narasimhan, and Jiří Friml. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors for Clathrin-Mediated Endocytosis.” <i>Cell Reports</i>. Cell Press, 2024. <a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">https://doi.org/10.1016/j.celrep.2024.114195</a>."},"isi":1,"das_tickbox":"1","day":"28","oa_version":"Published Version","researchdata_availability":"upon request","_id":"15374","status":"public","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.celrep.2024.114195","date_published":"2024-05-28T00:00:00Z","has_accepted_license":"1","ddc":["580"],"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) is an essential process of cargo uptake operating in all eukaryotes. In animals and yeast, BAR-SH3 domain proteins, endophilins and amphiphysins, function at the conclusion of CME to recruit factors for vesicle scission and uncoating. Arabidopsis thaliana contains the BAR-SH3 domain proteins SH3P1–SH3P3, but their role is poorly understood. Here, we identify SH3Ps as functional homologs of endophilin/amphiphysin. SH3P1–SH3P3 bind to discrete foci at the plasma membrane (PM), and SH3P2 recruits late to a subset of clathrin-coated pits. The SH3P2 PM recruitment pattern is nearly identical to its interactor, a putative uncoating factor, AUXILIN-LIKE1. Notably, SH3P1–SH3P3 are required for most of AUXILIN-LIKE1 recruitment to the PM. This indicates a plant-specific modification of CME, where BAR-SH3 proteins recruit auxilin-like uncoating factors rather than the uncoating phosphatases, synaptojanins. SH3P1–SH3P3 act redundantly in overall CME with the plant-specific endocytic adaptor TPLATE complex but not due to an SH3 domain in its TASH3 subunit."}],"file_date_updated":"2024-05-13T12:11:22Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-20T09:19:12Z"},{"date_updated":"2026-10-26T23:31:05Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"The developmental plasticity of the root system plays an essential role in the adaptation of plants to the environment. Among many other signals, auxin and its directional, intercellular transport are critical in regulating root growth and development. In particular, the PIN-FORMED2 (PIN2) auxin exporter acts as a key regulator of root gravitropic growth. Multiple regulators have been reported to be involved in PIN2-mediated root growth; however, our information remains incomplete. Here, we identified ROWY Bro1-domain proteins as important regulators of PIN2 sorting control. Genetic analysis revealed that Arabidopsis rowy1 single mutants and higher-order rowy1 rowy2 rowy3 triple mutants presented a wavy root growth phenotype. Cell biological experiments revealed that ROWY1 and PIN2 colocalized to the apical side of the plasma membrane in the root epidermis and that ROWYs are required for correct PM targeting of PIN2. In addition, ROWYs also affected PIN3 protein abundance in the stele, suggesting the potential involvement of additional PIN transporters as well as other proteins. A global transcriptome analysis revealed that ROWY genes are involved in the Fe2+ availability perception pathway. This work establishes ROWYs as important novel regulators of root gravitropic growth by connecting micronutrient availability to the proper subcellular targeting of PIN auxin transporters.","lang":"eng"}],"file_date_updated":"2024-09-17T09:44:29Z","ddc":["570"],"date_published":"2024-09-04T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1038/s42003-024-06747-9","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","_id":"18063","researchdata_availability":"yes (supplementary data)","oa_version":"Published Version","das_tickbox":"1","day":"04","isi":1,"acknowledgement":"We thank Drs. Erika Isono (University of Constance), Grégory Vert (University of Toulouse), and Liwen Jiang (The Chinese University of Hong Kong) for kindly sharing published Arabidopsis lines; Dr. Yuzhou Zhang (ISTA) for help with molecular cloning, and Drs. Melinda Abas (BOKU), Eugenia Russinova (Ghent University), and Zhaojun Ding (Shandong University) for valuable discussions. This work was supported by grants to S.T. from the National Natural Science Foundation of China (32321001), the USTC Research Funds of the Double First-Class Initiative (YD9100002016), the Research Funds from the Center for Advanced Interdisciplinary Science and Biomedicine of IHM, the Division of Life Sciences and Medicine, the University of Science and Technology of China (QYPY20220012), the Fundamental Research Funds for the Central Universities (WK9100000021), and start-up funding from the University of Science and Technology of China and the Chinese Academy of Sciences (GG9100007007, KY9100000026, KY9100000051, and KJ2070000079). J.S. was supported by the National Natural Science Foundation of China (31970181 and 32170342). J.F. was supported by Austrian Science Fund (FWF; projects I6123 and P37051-B).","citation":{"apa":"Peng, Y., Ji, K., Mao, Y., Wang, Y., Korbei, B., Luschnig, C., … Tan, S. (2024). Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>","ieee":"Y. Peng <i>et al.</i>, “Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis,” <i>Communications Biology</i>, vol. 7. Springer Nature, 2024.","ista":"Peng Y, Ji K, Mao Y, Wang Y, Korbei B, Luschnig C, Shen J, Benková E, Friml J, Tan S. 2024. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. Communications Biology. 7, 1085.","ama":"Peng Y, Ji K, Mao Y, et al. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. 2024;7. doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>","mla":"Peng, Yakun, et al. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>, vol. 7, 1085, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>.","short":"Y. Peng, K. Ji, Y. Mao, Y. Wang, B. Korbei, C. Luschnig, J. Shen, E. Benková, J. Friml, S. Tan, Communications Biology 7 (2024).","chicago":"Peng, Yakun, Kangkang Ji, Yanbo Mao, Yiqun Wang, Barbara Korbei, Christian Luschnig, Jinbo Shen, Eva Benková, Jiří Friml, and Shutang Tan. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>."},"year":"2024","publisher":"Springer Nature","dataavailabilitystatement":"The RNA-seq data for this study are available online at the NCBI Sequence Read Archive (accession number: PRJNA1116831). Uncropped images of the DNA gels are provided in the Supplementary Figs. 21 to 22. The source data used to generate the plots are provided in the Supplementary Data 2.","external_id":{"pmid":["39232040"],"isi":["001306499600002"]},"file":[{"content_type":"application/pdf","date_updated":"2024-09-17T09:44:29Z","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"7d66af41c90e73d1b8a375eb652a9561","file_name":"2024_CommBiology_Peng.pdf","file_size":7718758,"date_created":"2024-09-17T09:44:29Z","file_id":"18084","success":1}],"intvolume":"         7","scopus_import":"1","publication":"Communications Biology","author":[{"first_name":"Yakun","last_name":"Peng","full_name":"Peng, Yakun"},{"full_name":"Ji, Kangkang","last_name":"Ji","first_name":"Kangkang"},{"first_name":"Yanbo","last_name":"Mao","full_name":"Mao, Yanbo"},{"id":"82F537F2-B517-11E9-84D7-6433E6697425","last_name":"Wang","full_name":"Wang, Yiqun","first_name":"Yiqun"},{"full_name":"Korbei, Barbara","last_name":"Korbei","first_name":"Barbara"},{"first_name":"Christian","last_name":"Luschnig","full_name":"Luschnig, Christian"},{"first_name":"Jinbo","last_name":"Shen","full_name":"Shen, Jinbo"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","full_name":"Tan, Shutang"}],"pmid":1,"publication_identifier":{"eissn":["2399-3642"]},"title":"Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis","oa":1,"doi":"10.1038/s42003-024-06747-9","type":"journal_article","article_number":"1085","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"volume":7,"publication_status":"published","month":"09","supplementarymaterial":"yes","date_created":"2024-09-15T22:01:38Z","article_processing_charge":"Yes","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"}],"quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20117"}]}},{"doi":"10.1038/s41559-023-01981-6","type":"journal_article","publication_identifier":{"eissn":["2397-334X"]},"pmid":1,"oa":1,"title":"Pathogen evasion of social immunity","scopus_import":"1","corr_author":"1","author":[{"last_name":"Stock","id":"42462816-F248-11E8-B48F-1D18A9856A87","full_name":"Stock, Miriam","first_name":"Miriam"},{"full_name":"Milutinovic, Barbara","id":"2CDC32B8-F248-11E8-B48F-1D18A9856A87","last_name":"Milutinovic","first_name":"Barbara","orcid":"0000-0002-8214-4758"},{"last_name":"Hönigsberger","id":"953894f3-25bd-11ec-8556-f70a9d38ef60","full_name":"Hönigsberger, Michaela","first_name":"Michaela"},{"first_name":"Anna V","last_name":"Grasse","id":"406F989C-F248-11E8-B48F-1D18A9856A87","full_name":"Grasse, Anna V"},{"first_name":"Florian","id":"39523C54-F248-11E8-B48F-1D18A9856A87","last_name":"Wiesenhofer","full_name":"Wiesenhofer, Florian"},{"id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","last_name":"Kampleitner","full_name":"Kampleitner, Niklas","first_name":"Niklas"},{"full_name":"Narasimhan, Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","last_name":"Narasimhan","first_name":"Madhumitha","orcid":"0000-0002-8600-0671"},{"first_name":"Thomas","last_name":"Schmitt","full_name":"Schmitt, Thomas"},{"orcid":"0000-0002-2193-3868","first_name":"Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer","full_name":"Cremer, Sylvia"}],"publication":"Nature Ecology and Evolution","date_created":"2023-02-12T23:00:59Z","article_processing_charge":"No","quality_controlled":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/how-sneaky-germs-hide-from-ants/","description":"News on ISTA website","relation":"press_release"}]},"project":[{"grant_number":"771402","call_identifier":"H2020","_id":"2649B4DE-B435-11E9-9278-68D0E5697425","name":"Epidemics in ant societies on a chip"},{"name":"Host-Parasite Coevolution","_id":"25DAF0B2-B435-11E9-9278-68D0E5697425","grant_number":"CR-118/3-1"}],"department":[{"_id":"SyCr"},{"_id":"LifeSc"},{"_id":"JiFr"}],"volume":7,"language":[{"iso":"eng"}],"publication_status":"published","page":"450-460","month":"03","article_type":"original","fulldoi":"https://doi.org/10.1038/s41559-023-01981-6","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"date_published":"2023-03-01T00:00:00Z","has_accepted_license":"1","date_updated":"2025-04-14T07:47:53Z","ec_funded":1,"abstract":[{"lang":"eng","text":"Treating sick group members is a hallmark of collective disease defence in vertebrates and invertebrates alike. Despite substantial effects on pathogen fitness and epidemiology, it is still largely unknown how pathogens react to the selection pressure imposed by care intervention. Using social insects and pathogenic fungi, we here performed a serial passage experiment in the presence or absence of colony members, which provide social immunity by grooming off infectious spores from exposed individuals. We found specific effects on pathogen diversity, virulence and transmission. Under selection of social immunity, pathogens invested into higher spore production, but spores were less virulent. Notably, they also elicited a lower grooming response in colony members, compared with spores from the individual host selection lines. Chemical spore analysis suggested that the spores from social selection lines escaped the caregivers’ detection by containing lower levels of ergosterol, a key fungal membrane component. Experimental application of chemically pure ergosterol indeed induced sanitary grooming, supporting its role as a microbe-associated cue triggering host social immunity against fungal pathogens. By reducing this detection cue, pathogens were able to evade the otherwise very effective collective disease defences of their social hosts."}],"file_date_updated":"2023-08-16T11:54:59Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledged_ssus":[{"_id":"LifeSc"}],"file":[{"date_updated":"2023-08-16T11:54:59Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"8244f4650a0e7aeea488d1bcd4a31702","file_name":"2023_NatureEcoEvo_Stock.pdf","file_size":1600499,"date_created":"2023-08-16T11:54:59Z","file_id":"14069","success":1}],"intvolume":"         7","external_id":{"isi":["000924572800001"],"pmid":["36732670"]},"oa_version":"Published Version","status":"public","_id":"12543","acknowledgement":"We thank B. M. Steinwender, N. V. Meyling and J. Eilenberg for the fungal strains; J. Anaya-Rojas for statistical advice; the Social Immunity team at ISTA for ant collection and experimental help, in particular H. Leitner, and the ISTA Lab Support Facility for general laboratory support; D. Ebert, H. Schulenburg and J. Heinze for continued project discussion; and M. Sixt, R. Roemhild and the Social Immunity team for comments on the manuscript. The study was funded by the German Research Foundation (CR118/3-1) within the Framework of the Priority Program SPP 1399, and the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (No. 771402; EPIDEMICSonCHIP), both to S.C.","citation":{"chicago":"Stock, Miriam, Barbara Milutinovic, Michaela Hönigsberger, Anna V Grasse, Florian Wiesenhofer, Niklas Kampleitner, Madhumitha Narasimhan, Thomas Schmitt, and Sylvia Cremer. “Pathogen Evasion of Social Immunity.” <i>Nature Ecology and Evolution</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41559-023-01981-6\">https://doi.org/10.1038/s41559-023-01981-6</a>.","mla":"Stock, Miriam, et al. “Pathogen Evasion of Social Immunity.” <i>Nature Ecology and Evolution</i>, vol. 7, Springer Nature, 2023, pp. 450–60, doi:<a href=\"https://doi.org/10.1038/s41559-023-01981-6\">10.1038/s41559-023-01981-6</a>.","short":"M. Stock, B. Milutinovic, M. Hönigsberger, A.V. Grasse, F. Wiesenhofer, N. Kampleitner, M. Narasimhan, T. Schmitt, S. Cremer, Nature Ecology and Evolution 7 (2023) 450–460.","ista":"Stock M, Milutinovic B, Hönigsberger M, Grasse AV, Wiesenhofer F, Kampleitner N, Narasimhan M, Schmitt T, Cremer S. 2023. Pathogen evasion of social immunity. Nature Ecology and Evolution. 7, 450–460.","ama":"Stock M, Milutinovic B, Hönigsberger M, et al. Pathogen evasion of social immunity. <i>Nature Ecology and Evolution</i>. 2023;7:450-460. doi:<a href=\"https://doi.org/10.1038/s41559-023-01981-6\">10.1038/s41559-023-01981-6</a>","apa":"Stock, M., Milutinovic, B., Hönigsberger, M., Grasse, A. V., Wiesenhofer, F., Kampleitner, N., … Cremer, S. (2023). Pathogen evasion of social immunity. <i>Nature Ecology and Evolution</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41559-023-01981-6\">https://doi.org/10.1038/s41559-023-01981-6</a>","ieee":"M. Stock <i>et al.</i>, “Pathogen evasion of social immunity,” <i>Nature Ecology and Evolution</i>, vol. 7. Springer Nature, pp. 450–460, 2023."},"year":"2023","publisher":"Springer Nature","day":"01","isi":1},{"author":[{"last_name":"Jiang","full_name":"Jiang, Lihui","first_name":"Lihui"},{"last_name":"Yao","full_name":"Yao, Baolin","first_name":"Baolin"},{"full_name":"Zhang, Xiaoyan","last_name":"Zhang","first_name":"Xiaoyan"},{"first_name":"Lixia","full_name":"Wu, Lixia","last_name":"Wu"},{"first_name":"Qijing","last_name":"Fu","full_name":"Fu, Qijing"},{"first_name":"Yiting","full_name":"Zhao, Yiting","last_name":"Zhao"},{"first_name":"Yuxin","full_name":"Cao, Yuxin","last_name":"Cao"},{"first_name":"Ruomeng","last_name":"Zhu","full_name":"Zhu, Ruomeng"},{"first_name":"Xinqi","full_name":"Lu, Xinqi","last_name":"Lu"},{"last_name":"Huang","full_name":"Huang, Wuying","first_name":"Wuying"},{"first_name":"Jianping","last_name":"Zhao","full_name":"Zhao, Jianping"},{"full_name":"Li, Kuixiu","last_name":"Li","first_name":"Kuixiu"},{"first_name":"Shuanglu","last_name":"Zhao","full_name":"Zhao, Shuanglu"},{"first_name":"Li","last_name":"Han","full_name":"Han, Li"},{"first_name":"Xuan","last_name":"Zhou","full_name":"Zhou, Xuan"},{"first_name":"Chongyu","full_name":"Luo, Chongyu","last_name":"Luo"},{"full_name":"Zhu, Haiyan","last_name":"Zhu","first_name":"Haiyan"},{"first_name":"Jing","full_name":"Yang, Jing","last_name":"Yang"},{"last_name":"Huang","full_name":"Huang, Huichuan","first_name":"Huichuan"},{"first_name":"Zhengge","last_name":"Zhu","full_name":"Zhu, Zhengge"},{"full_name":"He, Xiahong","last_name":"He","first_name":"Xiahong"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"last_name":"Zhang","full_name":"Zhang, Zhongkai","first_name":"Zhongkai"},{"full_name":"Liu, Changning","last_name":"Liu","first_name":"Changning"},{"full_name":"Du, Yunlong","last_name":"Du","first_name":"Yunlong"}],"publication":"Plant Journal","scopus_import":"1","type":"journal_article","doi":"10.1111/tpj.16218","main_file_link":[{"url":"https://doi.org/10.1111/tpj.16218","open_access":"1"}],"oa":1,"title":"Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth","pmid":1,"publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"month":"07","publication_status":"published","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"volume":115,"page":"155-174","quality_controlled":"1","article_processing_charge":"No","date_created":"2023-04-30T22:01:06Z","abstract":[{"text":"Salicylic acid (SA) plays important roles in different aspects of plant development, including root growth, where auxin is also a major player by means of its asymmetric distribution. However, the mechanism underlying the effect of SA on the development of rice roots remains poorly understood. Here, we show that SA inhibits rice root growth by interfering with auxin transport associated with the OsPIN3t- and clathrin-mediated gene regulatory network (GRN). SA inhibits root growth as well as Brefeldin A-sensitive trafficking through a non-canonical SA signaling mechanism. Transcriptome analysis of rice seedlings treated with SA revealed that the OsPIN3t auxin transporter is at the center of a GRN involving the coat protein clathrin. The root growth and endocytic trafficking in both the pin3t and clathrin heavy chain mutants were SA insensitivity. SA inhibitory effect on the endocytosis of OsPIN3t was dependent on clathrin; however, the root growth and endocytic trafficking mediated by tyrphostin A23 (TyrA23) were independent of the pin3t mutant under SA treatment. These data reveal that SA affects rice root growth through the convergence of transcriptional and non-SA signaling mechanisms involving OsPIN3t-mediated auxin transport and clathrin-mediated trafficking as key components.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-06-18T17:29:30Z","OA_place":"publisher","fulldoi":"https://doi.org/10.1111/tpj.16218","article_type":"original","OA_type":"free access","date_published":"2023-07-01T00:00:00Z","ddc":["580"],"publisher":"Wiley","acknowledgement":"The authors thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese Academy of Sciences) for support with phytohormone measurement. Thanks also go to Professor Pieter. B. F. Ouwerkerk (Leiden University) and Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision of the rice lines NB-7B-70 and NB-7B-76 and wild-type NB-61-WT, Professor Zuhua He (Chinese Academy of Sciences) for provision of the rice OsNPR1-RNAi mutant, and Professor Yinong Yang (The Pennsylvania State University) for provision of the rice line NahG. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 32260085, 31460453, 31660501, 31860064, 31970609, 31801792 and 31960554), the Key Projects of the Applied Basic Research Plan of Yunnan Province (202301AS070082), the Major Special Program for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005), the Start-up fund from Xishuangbanna Tropical Botanical Garden, and ‘Top Talents Program in Science and Technology’ from Yunnan Province, the SRF for ROCS, SEM (Grant No. [2013] 1792), and the Major Science and Technology Project in Yunnan Province (202102AE090042 and 202202AE090036); and the young and middle-aged academic and technical leaders reserve talent program in Yunnan Province (202205AC160076).","year":"2023","citation":{"apa":"Jiang, L., Yao, B., Zhang, X., Wu, L., Fu, Q., Zhao, Y., … Du, Y. (2023). Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>","ieee":"L. Jiang <i>et al.</i>, “Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth,” <i>Plant Journal</i>, vol. 115, no. 1. Wiley, pp. 155–174, 2023.","ista":"Jiang L, Yao B, Zhang X, Wu L, Fu Q, Zhao Y, Cao Y, Zhu R, Lu X, Huang W, Zhao J, Li K, Zhao S, Han L, Zhou X, Luo C, Zhu H, Yang J, Huang H, Zhu Z, He X, Friml J, Zhang Z, Liu C, Du Y. 2023. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. 115(1), 155–174.","ama":"Jiang L, Yao B, Zhang X, et al. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. 2023;115(1):155-174. doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>","mla":"Jiang, Lihui, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>, vol. 115, no. 1, Wiley, 2023, pp. 155–74, doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>.","short":"L. Jiang, B. Yao, X. Zhang, L. Wu, Q. Fu, Y. Zhao, Y. Cao, R. Zhu, X. Lu, W. Huang, J. Zhao, K. Li, S. Zhao, L. Han, X. Zhou, C. Luo, H. Zhu, J. Yang, H. Huang, Z. Zhu, X. He, J. Friml, Z. Zhang, C. Liu, Y. Du, Plant Journal 115 (2023) 155–174.","chicago":"Jiang, Lihui, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao, Yuxin Cao, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>."},"isi":1,"day":"01","oa_version":"Published Version","status":"public","_id":"12878","intvolume":"       115","issue":"1","external_id":{"pmid":["37025008 "],"isi":["000971861400001"]}},{"external_id":{"pmid":["37010107"],"isi":["000971795800001"]},"issue":"3","file":[{"date_updated":"2023-07-13T13:26:33Z","content_type":"application/pdf","creator":"cchlebak","checksum":"5492e1d18ac3eaf202633d210fa0fb75","access_level":"open_access","relation":"main_file","file_name":"2023_PlantPhys_Chen.pdf","date_created":"2023-07-13T13:26:33Z","file_size":2076977,"success":1,"file_id":"13220"}],"intvolume":"       192","day":"01","isi":1,"acknowledgement":"We thank Dong liu for offering iron staining technique; ZhiChang Chen and Zhenbiao Yang for discussion; Dandan Zheng for earlier attempt; Liwen Jiang and Dingquan Huang for initial tests of the TEM experiment; John C. Sedbrook for a donation of sku5 and pSKU5::SKU5-GFP seeds; Catherine Perrot-Rechenmann and Ke Zhou for the donation of sks1, sks2, and sku5 sks1 seeds; Zengyu Liu and Zhongquan Lin for live-imaging microscopy assistance. We are grateful to Can Peng, and Xixia Li for helping with sample preparation, and taking TEM images, at the Center for Biological Imaging (CBI), Institute of Biophysics, Chinese Academy of Science.","citation":{"ista":"Chen C, Zhang Y, Cai J, Qiu Y, Li L, Gao C, Gao Y, Ke M, Wu S, Wei C, Chen J, Xu T, Friml J, Wang J, Li R, Chao D, Zhang B, Chen X, Gao Z. 2023. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. Plant Physiology. 192(3), 2243–2260.","ama":"Chen C, Zhang Y, Cai J, et al. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. <i>Plant Physiology</i>. 2023;192(3):2243-2260. doi:<a href=\"https://doi.org/10.1093/plphys/kiad207\">10.1093/plphys/kiad207</a>","ieee":"C. Chen <i>et al.</i>, “Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots,” <i>Plant Physiology</i>, vol. 192, no. 3. American Society of Plant Biologists, pp. 2243–2260, 2023.","apa":"Chen, C., Zhang, Y., Cai, J., Qiu, Y., Li, L., Gao, C., … Gao, Z. (2023). Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plphys/kiad207\">https://doi.org/10.1093/plphys/kiad207</a>","chicago":"Chen, C, Y Zhang, J Cai, Y Qiu, L Li, C Gao, Y Gao, et al. “Multi-Copper Oxidases SKU5 and SKS1 Coordinate Cell Wall Formation Using Apoplastic Redox-Based Reactions in Roots.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2023. <a href=\"https://doi.org/10.1093/plphys/kiad207\">https://doi.org/10.1093/plphys/kiad207</a>.","mla":"Chen, C., et al. “Multi-Copper Oxidases SKU5 and SKS1 Coordinate Cell Wall Formation Using Apoplastic Redox-Based Reactions in Roots.” <i>Plant Physiology</i>, vol. 192, no. 3, American Society of Plant Biologists, 2023, pp. 2243–60, doi:<a href=\"https://doi.org/10.1093/plphys/kiad207\">10.1093/plphys/kiad207</a>.","short":"C. Chen, Y. Zhang, J. Cai, Y. Qiu, L. Li, C. Gao, Y. Gao, M. Ke, S. Wu, C. Wei, J. Chen, T. Xu, J. Friml, J. Wang, R. Li, D. Chao, B. Zhang, X. Chen, Z. Gao, Plant Physiology 192 (2023) 2243–2260."},"year":"2023","publisher":"American Society of Plant Biologists","_id":"13213","status":"public","oa_version":"Published Version","ddc":["575"],"has_accepted_license":"1","date_published":"2023-07-01T00:00:00Z","fulldoi":"https://doi.org/10.1093/plphys/kiad207","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file_date_updated":"2023-07-13T13:26:33Z","abstract":[{"text":"The primary cell wall is a fundamental plant constituent that is flexible but sufficiently rigid to support the plant cell shape. Although many studies have demonstrated that reactive oxygen species (ROS) serve as important signaling messengers to modify the cell wall structure and affect cellular growth, the regulatory mechanism underlying the spatial-temporal regulation of ROS activity for cell wall maintenance remains largely unclear. Here, we demonstrate the role of the Arabidopsis (Arabidopsis thaliana) multicopper oxidase-like protein skewed 5 (SKU5) and its homolog SKU5-similar 1 (SKS1) in root cell wall formation through modulating ROS homeostasis. Loss of SKU5 and SKS1 function resulted in aberrant division planes, protruding cell walls, ectopic deposition of iron, and reduced nicotinamide adeninedinucleotide phosphate (NADPH) oxidase-dependent ROS overproduction in the root epidermis–cortex and cortex–endodermis junctions. A decrease in ROS level or inhibition of NADPH oxidase activity rescued the cell wall defects of sku5 sks1 double mutants. SKU5 and SKS1 proteins were activated by iron treatment, and iron over-accumulated in the walls between the root epidermis and cortex cell layers of sku5 sks1. The glycosylphosphatidylinositol-anchored motif was crucial for membrane association and functionality of SKU5 and SKS1. Overall, our results identified SKU5 and SKS1 as regulators of ROS at the cell surface for regulation of cell wall structure and root cell growth.","lang":"eng"}],"date_updated":"2024-10-21T06:01:27Z","quality_controlled":"1","date_created":"2023-07-12T07:32:58Z","article_processing_charge":"No","month":"07","page":"2243-2260","volume":192,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"pmid":1,"title":"Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots","oa":1,"doi":"10.1093/plphys/kiad207","type":"journal_article","publication":"Plant Physiology","author":[{"first_name":"C","last_name":"Chen","full_name":"Chen, C"},{"last_name":"Zhang","full_name":"Zhang, Y","first_name":"Y"},{"first_name":"J","last_name":"Cai","full_name":"Cai, J"},{"full_name":"Qiu, Y","last_name":"Qiu","first_name":"Y"},{"first_name":"L","full_name":"Li, L","last_name":"Li"},{"first_name":"C","last_name":"Gao","full_name":"Gao, C"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"first_name":"M","full_name":"Ke, M","last_name":"Ke"},{"full_name":"Wu, S","last_name":"Wu","first_name":"S"},{"full_name":"Wei, C","last_name":"Wei","first_name":"C"},{"full_name":"Chen, J","last_name":"Chen","first_name":"J"},{"first_name":"T","full_name":"Xu, T","last_name":"Xu"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"first_name":"J","last_name":"Wang","full_name":"Wang, J"},{"last_name":"Li","full_name":"Li, R","first_name":"R"},{"full_name":"Chao, D","last_name":"Chao","first_name":"D"},{"full_name":"Zhang, B","last_name":"Zhang","first_name":"B"},{"full_name":"Chen, X","last_name":"Chen","first_name":"X"},{"first_name":"Z","full_name":"Gao, Z","last_name":"Gao"}],"scopus_import":"1"},{"external_id":{"pmid":["37434303"],"isi":["001026321500001"]},"issue":"2","file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2024-01-29T11:21:43Z","access_level":"open_access","checksum":"6d9bbd45b8e7bb3ceee2586d447bacb2","relation":"main_file","file_size":974464,"date_created":"2024-01-29T11:21:43Z","file_name":"2023_NewPhytologist_Qi.pdf","success":1,"file_id":"14898"}],"intvolume":"       240","day":"01","isi":1,"acknowledgement":"We gratefully acknowledge our brave colleagues, whose excellent efforts kept the plant cAMP research going in the last two decades. The authors were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.","citation":{"ista":"Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling and beyond. New Phytologist. 240(2), 489–495.","ama":"Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. 2023;240(2):489-495. doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>","ieee":"L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling and beyond,” <i>New Phytologist</i>, vol. 240, no. 2. Wiley, pp. 489–495, 2023.","apa":"Qi, L., &#38; Friml, J. (2023). Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>","chicago":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>.","mla":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>, vol. 240, no. 2, Wiley, 2023, pp. 489–95, doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>.","short":"L. Qi, J. Friml, New Phytologist 240 (2023) 489–495."},"year":"2023","publisher":"Wiley","status":"public","_id":"13266","oa_version":"Published Version","ddc":["580"],"has_accepted_license":"1","date_published":"2023-10-01T00:00:00Z","fulldoi":"https://doi.org/10.1111/nph.19123","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2024-01-29T11:21:43Z","abstract":[{"text":"The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger in many mammalian signaling pathways. However, its role in plants remains not well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors and the demonstration of its importance for canonical auxin signaling put plant cAMP research back into spotlight. This insight briefly summarizes the well-established cAMP signaling pathways in mammalian cells and describes the turbulent and controversial history of plant cAMP research highlighting the major progress and the unresolved points. We also briefly review the current paradigm of auxin signaling to provide a background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling as well as impact of these discoveries on plant cAMP research in general.","lang":"eng"}],"date_updated":"2024-10-22T12:50:00Z","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"quality_controlled":"1","date_created":"2023-07-23T22:01:13Z","article_processing_charge":"Yes (via OA deal)","month":"10","page":"489-495","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"volume":240,"publication_status":"published","pmid":1,"publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"oa":1,"title":"Tale of cAMP as a second messenger in auxin signaling and beyond","doi":"10.1111/nph.19123","type":"journal_article","publication":"New Phytologist","author":[{"first_name":"Linlin","orcid":"0000-0001-5187-8401","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi","full_name":"Qi, Linlin"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"corr_author":"1","scopus_import":"1"},{"has_accepted_license":"1","date_published":"2023-10-01T00:00:00Z","ddc":["580"],"fulldoi":"https://doi.org/10.1016/j.pbi.2023.102443","article_type":"review","date_updated":"2025-09-09T12:54:16Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2023-11-02T17:03:20Z","abstract":[{"lang":"eng","text":"To respond to auxin, the chief orchestrator of their multicellularity, plants evolved multiple receptor systems and signal transduction cascades. Despite decades of research, however, we are still lacking a satisfactory synthesis of various auxin signaling mechanisms. The chief discrepancy and historical controversy of the field is that of rapid and slow auxin effects on plant physiology and development. How is it possible that ions begin to trickle across the plasma membrane as soon as auxin enters the cell, even though the best-characterized transcriptional auxin pathway can take effect only after tens of minutes? Recently, unexpected progress has been made in understanding this and other unknowns of auxin signaling. We provide a perspective on these exciting developments and concepts whose general applicability might have ramifications beyond auxin signaling."}],"issue":"10","external_id":{"isi":["001080095300001"],"pmid":["37666097"]},"intvolume":"        75","file":[{"file_name":"Fiedler CurrOpinOlantBiol 2023_revised.pdf","date_created":"2023-11-02T17:03:20Z","file_size":737872,"success":1,"file_id":"14482","content_type":"application/pdf","date_updated":"2023-11-02T17:03:20Z","creator":"amally","checksum":"1c476c3414d2dfb0c85db0cb6cfd8a28","access_level":"open_access","relation":"main_file"}],"status":"public","_id":"14313","oa_version":"Submitted Version","isi":1,"day":"01","publisher":"Elsevier","year":"2023","citation":{"ieee":"L. Fiedler and J. Friml, “Rapid auxin signaling: Unknowns old and new,” <i>Current Opinion in Plant Biology</i>, vol. 75, no. 10. Elsevier, 2023.","apa":"Fiedler, L., &#38; Friml, J. (2023). Rapid auxin signaling: Unknowns old and new. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2023.102443\">https://doi.org/10.1016/j.pbi.2023.102443</a>","ama":"Fiedler L, Friml J. Rapid auxin signaling: Unknowns old and new. <i>Current Opinion in Plant Biology</i>. 2023;75(10). doi:<a href=\"https://doi.org/10.1016/j.pbi.2023.102443\">10.1016/j.pbi.2023.102443</a>","ista":"Fiedler L, Friml J. 2023. Rapid auxin signaling: Unknowns old and new. Current Opinion in Plant Biology. 75(10), 102443.","mla":"Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and New.” <i>Current Opinion in Plant Biology</i>, vol. 75, no. 10, 102443, Elsevier, 2023, doi:<a href=\"https://doi.org/10.1016/j.pbi.2023.102443\">10.1016/j.pbi.2023.102443</a>.","short":"L. Fiedler, J. Friml, Current Opinion in Plant Biology 75 (2023).","chicago":"Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and New.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.pbi.2023.102443\">https://doi.org/10.1016/j.pbi.2023.102443</a>."},"acknowledgement":"The opening quote is not intended to reflect any political views of the authors. The authors by no means endorse the rhetoric of Donald Rumsfeld or the 2003 invasion of Iraq by the United States. Nevertheless, Rumsfeld's quote led to both public and academic debates on the concept of known and unknown unknowns, which can be applied to the recent unexpected developments in the auxin signaling field. We thank Linlin Qi and Huihuang Chen for their suggestions on figure presentation and inspiring discussions of TIR1/AFB signaling. Finally, we thank Aroosa Hussain for discussion of Greek mythology.","title":"Rapid auxin signaling: Unknowns old and new","oa":1,"pmid":1,"publication_identifier":{"issn":["1369-5266"]},"type":"journal_article","doi":"10.1016/j.pbi.2023.102443","article_number":"102443","corr_author":"1","scopus_import":"1","publication":"Current Opinion in Plant Biology","author":[{"first_name":"Lukas","full_name":"Fiedler, Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"article_processing_charge":"No","date_created":"2023-09-10T22:01:11Z","quality_controlled":"1","publication_status":"published","volume":75,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"month":"10"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2023-09-20T10:51:31Z","abstract":[{"lang":"eng","text":"Lateral roots are typically maintained at non-vertical angles with respect to gravity. These gravitropic setpoint angles are intriguing because their maintenance requires that roots are able to effect growth response both with and against the gravity vector, a phenomenon previously attributed to gravitropism acting against an antigravitropic offset mechanism. Here we show how the components mediating gravitropism in the vertical primary root—PINs and phosphatases acting upon them—are reconfigured in their regulation such that lateral root growth at a range of angles can be maintained. We show that the ability of Arabidopsis lateral roots to bend both downward and upward requires the generation of auxin asymmetries and is driven by angle-dependent variation in downward gravitropic auxin flux acting against angle-independent upward, antigravitropic flux. Further, we demonstrate a symmetry in auxin distribution in lateral roots at gravitropic setpoint angle that can be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters in the columella. These auxin fluxes are shifted by altering PIN protein phosphoregulation in the columella, either by introducing PIN3 phosphovariant versions or via manipulation of levels of the phosphatase subunit PP2A/RCN1. Finally, we show that auxin, in addition to driving lateral root directional growth, acts within the lateral root columella to induce more vertical growth by increasing RCN1 levels, causing a downward shift in PIN3 localization, thereby diminishing the magnitude of the upward, antigravitropic auxin flux."}],"date_updated":"2024-10-21T06:01:33Z","ddc":["580"],"has_accepted_license":"1","date_published":"2023-09-01T00:00:00Z","article_type":"original","fulldoi":"https://doi.org/10.1038/s41477-023-01478-x","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"day":"01","isi":1,"acknowledgement":"We thank D. Weijers, C. Schwechheimer and R. Offringa for generous sharing of published and unpublished materials and P. Masson for advice on the use of the ARL2 promoter. We are grateful to M. Del Bianco and O. Leyser for critical reading of the manuscript. This work was supported by the BBSRC (grants BB/N010124/1 and BB/R000859/1 to S.K.), the Gatsby Charitable Foundation and the Leverhulme Trust (RPG-2018-137 to S.K.).","year":"2023","citation":{"ista":"Roychoudhry S, Sageman-Furnas K, Wolverton C, Grones P, Tan S, Molnar G, De Angelis M, Goodman H, Capstaff N, JPB L, Mullen J, Hangarter R, Friml J, Kepinski S. 2023. Antigravitropic PIN polarization maintains non-vertical growth in lateral roots. Nature Plants. 9, 1500–1513.","ama":"Roychoudhry S, Sageman-Furnas K, Wolverton C, et al. Antigravitropic PIN polarization maintains non-vertical growth in lateral roots. <i>Nature Plants</i>. 2023;9:1500-1513. doi:<a href=\"https://doi.org/10.1038/s41477-023-01478-x\">10.1038/s41477-023-01478-x</a>","ieee":"S. Roychoudhry <i>et al.</i>, “Antigravitropic PIN polarization maintains non-vertical growth in lateral roots,” <i>Nature Plants</i>, vol. 9. Springer Nature, pp. 1500–1513, 2023.","apa":"Roychoudhry, S., Sageman-Furnas, K., Wolverton, C., Grones, P., Tan, S., Molnar, G., … Kepinski, S. (2023). Antigravitropic PIN polarization maintains non-vertical growth in lateral roots. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-023-01478-x\">https://doi.org/10.1038/s41477-023-01478-x</a>","chicago":"Roychoudhry, S, K Sageman-Furnas, C Wolverton, Peter Grones, Shutang Tan, Gergely Molnar, M De Angelis, et al. “Antigravitropic PIN Polarization Maintains Non-Vertical Growth in Lateral Roots.” <i>Nature Plants</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41477-023-01478-x\">https://doi.org/10.1038/s41477-023-01478-x</a>.","short":"S. Roychoudhry, K. Sageman-Furnas, C. Wolverton, P. Grones, S. Tan, G. Molnar, M. De Angelis, H. Goodman, N. Capstaff, L. JPB, J. Mullen, R. Hangarter, J. Friml, S. Kepinski, Nature Plants 9 (2023) 1500–1513.","mla":"Roychoudhry, S., et al. “Antigravitropic PIN Polarization Maintains Non-Vertical Growth in Lateral Roots.” <i>Nature Plants</i>, vol. 9, Springer Nature, 2023, pp. 1500–13, doi:<a href=\"https://doi.org/10.1038/s41477-023-01478-x\">10.1038/s41477-023-01478-x</a>."},"publisher":"Springer Nature","_id":"14339","status":"public","oa_version":"Published Version","external_id":{"pmid":["37666965"],"isi":["001069238800014"]},"file":[{"relation":"main_file","access_level":"open_access","checksum":"3d6d5d5abb937c14a5f6f0afba3b8624","date_updated":"2023-09-20T10:51:31Z","content_type":"application/pdf","creator":"dernst","file_id":"14351","success":1,"file_name":"2023_NaturePlants_Roychoudhry.pdf","date_created":"2023-09-20T10:51:31Z","file_size":9647103}],"intvolume":"         9","publication":"Nature Plants","author":[{"full_name":"Roychoudhry, S","last_name":"Roychoudhry","first_name":"S"},{"last_name":"Sageman-Furnas","full_name":"Sageman-Furnas, K","first_name":"K"},{"full_name":"Wolverton, C","last_name":"Wolverton","first_name":"C"},{"full_name":"Grones, Peter","last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"},{"last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","first_name":"Gergely"},{"full_name":"De Angelis, M","last_name":"De Angelis","first_name":"M"},{"last_name":"Goodman","full_name":"Goodman, HL","first_name":"HL"},{"first_name":"N","full_name":"Capstaff, N","last_name":"Capstaff"},{"last_name":"JPB","full_name":"JPB, Lloyd","first_name":"Lloyd"},{"first_name":"J","last_name":"Mullen","full_name":"Mullen, J"},{"full_name":"Hangarter, R","last_name":"Hangarter","first_name":"R"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"first_name":"S","full_name":"Kepinski, S","last_name":"Kepinski"}],"scopus_import":"1","pmid":1,"publication_identifier":{"issn":["2055-0278"]},"oa":1,"title":"Antigravitropic PIN polarization maintains non-vertical growth in lateral roots","doi":"10.1038/s41477-023-01478-x","type":"journal_article","month":"09","page":"1500-1513","language":[{"iso":"eng"}],"volume":9,"department":[{"_id":"JiFr"}],"publication_status":"published","quality_controlled":"1","date_created":"2023-09-15T09:56:01Z","article_processing_charge":"Yes (in subscription journal)"},{"month":"12","volume":74,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","page":"6889-6892","quality_controlled":"1","date_created":"2023-12-24T23:00:53Z","article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"Marta","last_name":"Del Bianco","full_name":"Del Bianco, Marta"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lucia","full_name":"Strader, Lucia","last_name":"Strader"},{"last_name":"Kepinski","full_name":"Kepinski, Stefan","first_name":"Stefan"}],"publication":"Journal of Experimental Botany","scopus_import":"1","doi":"10.1093/jxb/erad420","type":"journal_article","pmid":1,"publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"oa":1,"title":"Auxin research: Creating tools for a greener future","year":"2023","citation":{"short":"M. Del Bianco, J. Friml, L. Strader, S. Kepinski, Journal of Experimental Botany 74 (2023) 6889–6892.","mla":"Del Bianco, Marta, et al. “Auxin Research: Creating Tools for a Greener Future.” <i>Journal of Experimental Botany</i>, vol. 74, no. 22, Oxford University Press, 2023, pp. 6889–92, doi:<a href=\"https://doi.org/10.1093/jxb/erad420\">10.1093/jxb/erad420</a>.","chicago":"Del Bianco, Marta, Jiří Friml, Lucia Strader, and Stefan Kepinski. “Auxin Research: Creating Tools for a Greener Future.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/jxb/erad420\">https://doi.org/10.1093/jxb/erad420</a>.","apa":"Del Bianco, M., Friml, J., Strader, L., &#38; Kepinski, S. (2023). Auxin research: Creating tools for a greener future. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/erad420\">https://doi.org/10.1093/jxb/erad420</a>","ieee":"M. Del Bianco, J. Friml, L. Strader, and S. Kepinski, “Auxin research: Creating tools for a greener future,” <i>Journal of Experimental Botany</i>, vol. 74, no. 22. Oxford University Press, pp. 6889–6892, 2023.","ama":"Del Bianco M, Friml J, Strader L, Kepinski S. Auxin research: Creating tools for a greener future. <i>Journal of Experimental Botany</i>. 2023;74(22):6889-6892. doi:<a href=\"https://doi.org/10.1093/jxb/erad420\">10.1093/jxb/erad420</a>","ista":"Del Bianco M, Friml J, Strader L, Kepinski S. 2023. Auxin research: Creating tools for a greener future. Journal of Experimental Botany. 74(22), 6889–6892."},"publisher":"Oxford University Press","day":"01","isi":1,"oa_version":"Published Version","status":"public","_id":"14709","file":[{"file_id":"14724","success":1,"file_name":"2023_JourExperimentalBotany_DelBianco.pdf","date_created":"2024-01-02T09:23:57Z","file_size":425194,"relation":"main_file","checksum":"f66fb960fd791dea53fd0e087f2fbbe8","access_level":"open_access","date_updated":"2024-01-02T09:23:57Z","content_type":"application/pdf","creator":"dernst"}],"intvolume":"        74","external_id":{"pmid":["38038239"],"isi":["001145889700001"]},"issue":"22","abstract":[{"text":"Amid the delays due to the global pandemic, in early October 2022, the auxin community gathered in the idyllic peninsula of Cavtat, Croatia. More than 170 scientists from across the world converged to discuss the latest advancements in fundamental and applied research in the field. The topics, from signalling and transport to plant architecture and response to the environment, show how auxin research must bridge from the molecular realm to macroscopic developmental responses. This is mirrored in this collection of reviews, contributed by participants of the Auxin 2022 meeting.","lang":"eng"}],"file_date_updated":"2024-01-02T09:23:57Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-09T14:06:39Z","fulldoi":"https://doi.org/10.1093/jxb/erad420","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"has_accepted_license":"1","date_published":"2023-12-01T00:00:00Z"},{"date_created":"2024-01-10T09:24:35Z","article_processing_charge":"Yes","quality_controlled":"1","department":[{"_id":"JiFr"}],"volume":24,"language":[{"iso":"eng"}],"publication_status":"published","month":"11","doi":"10.3390/ijms242216527","type":"journal_article","publication_identifier":{"issn":["1422-0067"]},"pmid":1,"oa":1,"title":"Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3","article_number":"16527","corr_author":"1","author":[{"first_name":"Anastasiia","last_name":"Teplova","id":"e3736151-106c-11ec-b916-c2558e2762c6","full_name":"Teplova, Anastasiia"},{"full_name":"Pigidanov, Artemii A.","last_name":"Pigidanov","first_name":"Artemii A."},{"first_name":"Marina V.","full_name":"Serebryakova, Marina V.","last_name":"Serebryakova"},{"first_name":"Sergei A.","full_name":"Golyshev, Sergei A.","last_name":"Golyshev"},{"full_name":"Galiullina, Raisa A.","last_name":"Galiullina","first_name":"Raisa A."},{"last_name":"Chichkova","full_name":"Chichkova, Nina V.","first_name":"Nina V."},{"first_name":"Andrey B.","last_name":"Vartapetian","full_name":"Vartapetian, Andrey B."}],"publication":"International Journal of Molecular Sciences","file":[{"success":1,"file_id":"14791","file_name":"2023_IJMS_Teplova.pdf","file_size":2637784,"date_created":"2024-01-10T13:39:42Z","checksum":"4df7d206ba022b7f54eff1f0aec1659a","access_level":"open_access","relation":"main_file","date_updated":"2024-01-10T13:39:42Z","content_type":"application/pdf","creator":"dernst"}],"intvolume":"        24","external_id":{"pmid":["38003717"],"isi":["001113792600001"]},"issue":"22","oa_version":"Published Version","_id":"14776","status":"public","citation":{"apa":"Teplova, A., Pigidanov, A. A., Serebryakova, M. V., Golyshev, S. A., Galiullina, R. A., Chichkova, N. V., &#38; Vartapetian, A. B. (2023). Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms242216527\">https://doi.org/10.3390/ijms242216527</a>","ieee":"A. Teplova <i>et al.</i>, “Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3,” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 22. MDPI, 2023.","ista":"Teplova A, Pigidanov AA, Serebryakova MV, Golyshev SA, Galiullina RA, Chichkova NV, Vartapetian AB. 2023. Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. International Journal of Molecular Sciences. 24(22), 16527.","ama":"Teplova A, Pigidanov AA, Serebryakova MV, et al. Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. <i>International Journal of Molecular Sciences</i>. 2023;24(22). doi:<a href=\"https://doi.org/10.3390/ijms242216527\">10.3390/ijms242216527</a>","short":"A. Teplova, A.A. Pigidanov, M.V. Serebryakova, S.A. Golyshev, R.A. Galiullina, N.V. Chichkova, A.B. Vartapetian, International Journal of Molecular Sciences 24 (2023).","mla":"Teplova, Anastasiia, et al. “Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal from Tobacco Calreticulin-3.” <i>International Journal of Molecular Sciences</i>, vol. 24, no. 22, 16527, MDPI, 2023, doi:<a href=\"https://doi.org/10.3390/ijms242216527\">10.3390/ijms242216527</a>.","chicago":"Teplova, Anastasiia, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, and Andrey B. Vartapetian. “Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal from Tobacco Calreticulin-3.” <i>International Journal of Molecular Sciences</i>. MDPI, 2023. <a href=\"https://doi.org/10.3390/ijms242216527\">https://doi.org/10.3390/ijms242216527</a>."},"year":"2023","acknowledgement":"We thank C.U.T. Hellen for critically reading the manuscript. The MALDI MS facility and CLSM became available to us in the framework of Moscow State University Development Programs PNG 5.13 and PNR 5.13.\r\nThis work was funded by the Russian Science Foundation, grant numbers 19-14-00010 and 22-14-00071.","publisher":"MDPI","day":"01","isi":1,"fulldoi":"https://doi.org/10.3390/ijms242216527","article_type":"original","keyword":["Inorganic Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Computer Science Applications","Spectroscopy","Molecular Biology","General Medicine","Catalysis"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"has_accepted_license":"1","date_published":"2023-11-01T00:00:00Z","date_updated":"2024-10-09T21:07:49Z","file_date_updated":"2024-01-10T13:39:42Z","abstract":[{"lang":"eng","text":"Soluble chaperones residing in the endoplasmic reticulum (ER) play vitally important roles in folding and quality control of newly synthesized proteins that transiently pass through the ER en route to their final destinations. These soluble residents of the ER are themselves endowed with an ER retrieval signal that enables the cell to bring the escaped residents back from the Golgi. Here, by using purified proteins, we showed that Nicotiana tabacum phytaspase, a plant aspartate-specific protease, introduces two breaks at the C-terminus of the N. tabacum ER resident calreticulin-3. These cleavages resulted in removal of either a dipeptide or a hexapeptide from the C-terminus of calreticulin-3 encompassing part or all of the ER retrieval signal. Consistently, expression of the calreticulin-3 derivative mimicking the phytaspase cleavage product in Nicotiana benthamiana cells demonstrated loss of the ER accumulation of the protein. Notably, upon its escape from the ER, calreticulin-3 was further processed by an unknown protease(s) to generate the free N-terminal (N) domain of calreticulin-3, which was ultimately secreted into the apoplast. Our study thus identified a specific proteolytic enzyme capable of precise detachment of the ER retrieval signal from a plant ER resident protein, with implications for the further fate of the escaped resident."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"fulldoi":"https://doi.org/10.1016/j.xplc.2023.100632","article_type":"original","date_published":"2023-11-13T00:00:00Z","has_accepted_license":"1","ddc":["580"],"date_updated":"2026-07-06T12:59:25Z","abstract":[{"lang":"eng","text":"The phytohormone auxin plays central roles in many growth and developmental processes in plants. Development of chemical tools targeting the auxin pathway is useful for both plant biology and agriculture. Here we reveal that naproxen, a synthetic compound with anti-inflammatory activity in humans, acts as an auxin transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated developmental processes. Additional cellular and biochemical evidence indicates that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux. Moreover, biochemical and structural analyses confirm that naproxen binds directly to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate. Thus, by combining cellular, biochemical, and structural approaches, this study clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying mechanisms. Further use of this compound may advance our understanding of the molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in auxin biology and agriculture."}],"file_date_updated":"2024-01-30T10:54:40Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"         4","file":[{"access_level":"open_access","checksum":"f8ef92af6096834f91ce38587fb1db9f","relation":"main_file","date_updated":"2024-01-30T10:54:40Z","content_type":"application/pdf","creator":"dernst","success":1,"file_id":"14900","file_name":"2023_PlantCommunications_Xia.pdf","date_created":"2024-01-30T10:54:40Z","file_size":1434862}],"issue":"6","external_id":{"isi":["001113003000001"],"pmid":["37254481"]},"oa_version":"Published Version","_id":"13209","status":"public","publisher":"Elsevier","year":"2023","acknowledgement":"This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB37020103 to Linfeng Sun); research funds from the Center for Advanced Interdisciplinary Science\r\nand Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China (QYPY20220012 to S.T.); start-up funding from the University of Science and Technology of China and the\r\nChinese Academy of Sciences (GG9100007007, KY9100000026,KY9100000051, and KJ2070000079 to S.T.); the National Natural Science Foundation of China (31900885 to X.L. and 31870732 to Linfeng Sun); the Natural Science Foundation of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun); the Fundamental Research Funds for the Central Universities (WK9100000021 to S.T. and WK9100000031 to Linfeng Sun); and the USTC Research Funds of the Double First-Class Initiative (YD9100002016 to S.T. and YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation.We thank Dr. Yang Zhao for sharing published materials (Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences) and the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We are grateful to Y. Gao and all other staff members for their technical support on cryo-EM data collection. ","citation":{"chicago":"Xia, Jing, Mengjuan Kong, Zhisen Yang, Lianghanxiao Sun, Yakun Peng, Yanbo Mao, Hong Wei, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.xplc.2023.100632\">https://doi.org/10.1016/j.xplc.2023.100632</a>.","mla":"Xia, Jing, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>, vol. 4, no. 6, 100632, Elsevier, 2023, doi:<a href=\"https://doi.org/10.1016/j.xplc.2023.100632\">10.1016/j.xplc.2023.100632</a>.","short":"J. Xia, M. Kong, Z. Yang, L. Sun, Y. Peng, Y. Mao, H. Wei, W. Ying, Y. Gao, J. Friml, J. Weng, X. Liu, L. Sun, S. Tan, Plant Communications 4 (2023).","ama":"Xia J, Kong M, Yang Z, et al. Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen. <i>Plant Communications</i>. 2023;4(6). doi:<a href=\"https://doi.org/10.1016/j.xplc.2023.100632\">10.1016/j.xplc.2023.100632</a>","ista":"Xia J, Kong M, Yang Z, Sun L, Peng Y, Mao Y, Wei H, Ying W, Gao Y, Friml J, Weng J, Liu X, Sun L, Tan S. 2023. Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen. Plant Communications. 4(6), 100632.","ieee":"J. Xia <i>et al.</i>, “Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen,” <i>Plant Communications</i>, vol. 4, no. 6. Elsevier, 2023.","apa":"Xia, J., Kong, M., Yang, Z., Sun, L., Peng, Y., Mao, Y., … Tan, S. (2023). Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen. <i>Plant Communications</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xplc.2023.100632\">https://doi.org/10.1016/j.xplc.2023.100632</a>"},"isi":1,"day":"13","das_tickbox":"1","type":"journal_article","doi":"10.1016/j.xplc.2023.100632","title":"Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory drug naproxen","oa":1,"publication_identifier":{"eissn":["2590-3462"]},"pmid":1,"article_number":"100632","scopus_import":"1","author":[{"last_name":"Xia","full_name":"Xia, Jing","first_name":"Jing"},{"first_name":"Mengjuan","last_name":"Kong","full_name":"Kong, Mengjuan"},{"first_name":"Zhisen","full_name":"Yang, Zhisen","last_name":"Yang"},{"full_name":"Sun, Lianghanxiao","last_name":"Sun","first_name":"Lianghanxiao"},{"first_name":"Yakun","last_name":"Peng","full_name":"Peng, Yakun"},{"full_name":"Mao, Yanbo","last_name":"Mao","first_name":"Yanbo"},{"first_name":"Hong","full_name":"Wei, Hong","last_name":"Wei"},{"first_name":"Wei","last_name":"Ying","full_name":"Ying, Wei"},{"first_name":"Yongxiao","full_name":"Gao, Yongxiao","last_name":"Gao"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"first_name":"Jianping","full_name":"Weng, Jianping","last_name":"Weng"},{"last_name":"Liu","full_name":"Liu, Xin","first_name":"Xin"},{"first_name":"Linfeng","full_name":"Sun, Linfeng","last_name":"Sun"},{"full_name":"Tan, Shutang","last_name":"Tan","first_name":"Shutang"}],"publication":"Plant Communications","article_processing_charge":"Yes","date_created":"2023-07-12T07:32:00Z","quality_controlled":"1","publication_status":"published","volume":4,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"month":"11"},{"file_date_updated":"2023-12-13T23:30:03Z","abstract":[{"lang":"eng","text":"As a crucial nitrogen source, nitrate (NO3−) is a key nutrient for plants. Accordingly, root systems adapt to maximize NO3− availability, a developmental regulation also involving the phytohormone auxin. Nonetheless, the molecular mechanisms underlying this regulation remain poorly understood. Here, we identify low-nitrate-resistant mutant (lonr) in Arabidopsis (Arabidopsis thaliana), whose root growth fails to adapt to low-NO3− conditions. lonr2 is defective in the high-affinity NO3− transporter NRT2.1. lonr2 (nrt2.1) mutants exhibit defects in polar auxin transport, and their low-NO3−-induced root phenotype depends on the PIN7 auxin exporter activity. NRT2.1 directly associates with PIN7 and antagonizes PIN7-mediated auxin efflux depending on NO3− levels. These results reveal a mechanism by which NRT2.1 in response to NO3− limitation directly regulates auxin transport activity and, thus, root growth. This adaptive mechanism contributes to the root developmental plasticity to help plants cope with changes in NO3− availability."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-12-13T23:30:04Z","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"fulldoi":"https://doi.org/10.1073/pnas.2221313120","article_type":"original","has_accepted_license":"1","date_published":"2023-06-12T00:00:00Z","ddc":["570"],"publisher":"National Academy of Sciences","citation":{"ista":"Wang Y, Yuan Z, Wang J, Xiao H, Wan L, Li L, Guo Y, Gong Z, Friml J, Zhang J. 2023. The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation. Proceedings of the National Academy of Sciences of the United States of America. 120(25), e2221313120.","ama":"Wang Y, Yuan Z, Wang J, et al. The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2023;120(25). doi:<a href=\"https://doi.org/10.1073/pnas.2221313120\">10.1073/pnas.2221313120</a>","ieee":"Y. Wang <i>et al.</i>, “The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 120, no. 25. National Academy of Sciences, 2023.","apa":"Wang, Y., Yuan, Z., Wang, J., Xiao, H., Wan, L., Li, L., … Zhang, J. (2023). The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2221313120\">https://doi.org/10.1073/pnas.2221313120</a>","chicago":"Wang, Yalu, Zhi Yuan, Jinyi Wang, Huixin Xiao, Lu Wan, Lanxin Li, Yan Guo, Zhizhong Gong, Jiří Friml, and Jing Zhang. “The Nitrate Transporter NRT2.1 Directly Antagonizes PIN7-Mediated Auxin Transport for Root Growth Adaptation.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2023. <a href=\"https://doi.org/10.1073/pnas.2221313120\">https://doi.org/10.1073/pnas.2221313120</a>.","mla":"Wang, Yalu, et al. “The Nitrate Transporter NRT2.1 Directly Antagonizes PIN7-Mediated Auxin Transport for Root Growth Adaptation.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 120, no. 25, e2221313120, National Academy of Sciences, 2023, doi:<a href=\"https://doi.org/10.1073/pnas.2221313120\">10.1073/pnas.2221313120</a>.","short":"Y. Wang, Z. Yuan, J. Wang, H. Xiao, L. Wan, L. Li, Y. Guo, Z. Gong, J. Friml, J. Zhang, Proceedings of the National Academy of Sciences of the United States of America 120 (2023)."},"year":"2023","acknowledgement":"We are grateful to Caifu Jiang for providing ethyl metha-nesulfonate- mutagenized population, Yi Wang for providing Xenopus oocytes, Jun Fan and Zhaosheng Kong for providing tobacco BY- 2 cells, and Claus Schwechheimer, Alain Gojon, and Shutang Tan for helpful discussions. This work was supported by the National Key Research and Development Program of China (2021YFF1000500), the  National  Natural  Science  Foundation  of  China  (32170265  and  32022007),  Hainan  Provincial  Natural  Science  Foundation  of  China  (323CXTD379),  Chinese  Universities  Scientific  Fund  (2023TC019),  Beijing  Municipal  Natural  Science  Foundation  (5192011),  Beijing  Outstanding  University  Discipline  Program,  and  China Postdoctoral Science Foundation (BH2020259460).","isi":1,"day":"12","oa_version":"Published Version","_id":"13201","status":"public","intvolume":"       120","file":[{"content_type":"application/pdf","date_updated":"2023-12-13T23:30:03Z","creator":"alisjak","access_level":"open_access","checksum":"d800e06252eaefba28531fa9440f23f0","relation":"main_file","file_name":"2023_PNAS_Wang.pdf","file_size":5244581,"date_created":"2023-07-10T08:48:40Z","embargo":"2023-12-12","file_id":"13204"}],"issue":"25","external_id":{"pmid":["37307446"],"isi":["001030689600003"]},"author":[{"first_name":"Yalu","last_name":"Wang","full_name":"Wang, Yalu"},{"first_name":"Zhi","last_name":"Yuan","full_name":"Yuan, Zhi"},{"first_name":"Jinyi","full_name":"Wang, Jinyi","last_name":"Wang"},{"first_name":"Huixin","full_name":"Xiao, Huixin","last_name":"Xiao"},{"first_name":"Lu","last_name":"Wan","full_name":"Wan, Lu"},{"first_name":"Lanxin","orcid":"0000-0002-5607-272X","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"last_name":"Guo","full_name":"Guo, Yan","first_name":"Yan"},{"first_name":"Zhizhong","last_name":"Gong","full_name":"Gong, Zhizhong"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Zhang, Jing","last_name":"Zhang","first_name":"Jing"}],"publication":"Proceedings of the National Academy of Sciences of the United States of America","scopus_import":"1","article_number":"e2221313120","type":"journal_article","doi":"10.1073/pnas.2221313120","oa":1,"title":"The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation","publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"pmid":1,"month":"06","publication_status":"published","department":[{"_id":"JiFr"}],"volume":120,"language":[{"iso":"eng"}],"quality_controlled":"1","article_processing_charge":"No","date_created":"2023-07-09T22:01:12Z"}]