--- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20187' abstract: - lang: eng text: Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development. acknowledged_ssus: - _id: Bio - _id: EM-Fac - _id: LifeSc acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007)." article_number: e70396 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: David full_name: Babic, David id: db566d23-f6e0-11ea-865d-e6f270e968e7 last_name: Babic - first_name: Rashed full_name: Abualia, Rashed id: 4827E134-F248-11E8-B48F-1D18A9856A87 last_name: Abualia orcid: 0000-0002-9357-9415 - first_name: Lukas full_name: Fiedler, Lukas id: 7c417475-8972-11ed-ae7b-8b674ca26986 last_name: Fiedler - first_name: Linlin full_name: Qi, Linlin id: 44B04502-A9ED-11E9-B6FC-583AE6697425 last_name: Qi orcid: 0000-0001-5187-8401 - first_name: Frédérique full_name: Tellier, Frédérique last_name: Tellier - first_name: Adrijana full_name: Smoljan, Adrijana id: cced8a85-223e-11ed-af04-b0596c55053b last_name: Smoljan - first_name: Hana full_name: Rakusova, Hana id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA last_name: Rakusova - first_name: Petr full_name: Valošek, Petr id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87 last_name: Valošek - first_name: Huibin full_name: Han, Huibin id: 31435098-F248-11E8-B48F-1D18A9856A87 last_name: Han - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Jean Denis full_name: Faure, Jean Denis last_name: Faure - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396 apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. Wiley. https://doi.org/10.1111/tpj.70396 chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396. ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant Journal, vol. 123, no. 3. Wiley, 2025. ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396. mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396. short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025). corr_author: '1' date_created: 2025-08-17T22:01:36Z date_published: 2025-08-01T00:00:00Z date_updated: 2026-10-26T23:31:07Z day: '01' ddc: - '580' department: - _id: EvBe - _id: JiFr - _id: GradSch doi: 10.1111/tpj.70396 external_id: isi: - '001547884300001' pmid: - '40782342' file: - access_level: open_access checksum: 1cdc3341d2d23101abca72521f1f23cb content_type: application/pdf creator: dernst date_created: 2025-09-01T14:09:31Z date_updated: 2025-09-01T14:09:31Z file_id: '20264' file_name: 2025_PlantJournal_Babic.pdf file_size: 5791111 relation: main_file success: 1 file_date_updated: 2025-09-01T14:09:31Z fulldoi: https://doi.org/10.1111/tpj.70396 has_accepted_license: '1' intvolume: ' 123' isi: 1 issue: '3' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '08' oa: 1 oa_version: Published Version pmid: 1 project: - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis publication: Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley quality_controlled: '1' related_material: record: - id: '20362' relation: dissertation_contains status: public scopus_import: '1' status: public title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 123 year: '2025' ... --- OA_place: publisher _id: '20362' abstract: - lang: eng text: "Arabidopsis embryo has long served as a model system for understanding how plant body \r\npatterning and axis establishment arise. Previous work established that auxin flows, directed \r\nby polar transport and reinforced by local biosynthesis, underpin axes formation, while recent \r\nstudies suggest that maternal tissue, membrane composition and surface receptor signaling add \r\nfurther regulatory layers. In this thesis, we dissect the layers of auxin-mediated processes which \r\nguide plant embryonic and post-embryonic development. We review methodological advances \r\nthat allow the embryo to be imaged and studied. These tools offer exciting venues for future \r\naddressing of outstanding research questions. Furthermore, we dissect the contribution of \r\nmaternal auxin for early embryo patterning. Targeted auxin scavenging in the integuments \r\nlowers the hormone reaching the proembryo, destabilizes PIN7 in the suspensor and weakens \r\nthe initial apical maximum. The resulting deficit dampens downstream embryonic auxin \r\nbiosynthesis, as well as auxin flow towards the root pole, indicating that maternally provided \r\nauxin controls auxin flows in the embryos on a larger scale than previously understood. \r\nIntriguingly, we uncover a prolonged maternal effect mediated by the receptor-like kinases \r\nTMK1 and TMK4. Reciprocal crosses and transgene analyses hint that these genes could be \r\npreferentially maternally-derived in both embryos and seedlings. Loss of maternal TMK \r\nactivity arrests embryos, affects seedling development and persists into adult growth, \r\nsuggesting that maternal effect could shape development beyond fertilization and early \r\nembryogenesis. Finally, we describe the role of very-long-chain fatty acids and membrane \r\ncomposition on PIN proteins and their auxin-induced relocalization. Disruption in this lipid \r\nmetabolism triggers widespread patterning errors. \r\nTogether, these results reposition early plant patterning as a hierarchical cascade that begins \r\nwith maternal auxin, gets amplified by auxin transport and biosynthesis, potentially modulated \r\nby maternal receptor signaling and could ultimately be gated by the biophysical properties of \r\nthe membrane. By integrating auxin supply, genetic imprinting and lipid-dependent trafficking \r\ninto a single narrative, this thesis helps shifting the approach of viewing the embryo as an \r\nautonomous unit to regarding it as a dynamic system, continuously sculpted by its maternal \r\ncontext." acknowledged_ssus: - _id: Bio - _id: LifeSc alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: David full_name: Babic, David id: db566d23-f6e0-11ea-865d-e6f270e968e7 last_name: Babic citation: ama: Babic D. Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. 2025. doi:10.15479/AT-ISTA-20362 apa: Babic, D. (2025). Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20362 chicago: Babic, David. “Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20362. ieee: D. Babic, “Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2025. ista: Babic D. 2025. Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. Institute of Science and Technology Austria. mla: Babic, David. Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20362. short: D. Babic, Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-09-17T13:28:01Z date_published: 2025-09-18T00:00:00Z date_updated: 2026-10-16T07:59:32Z day: '18' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr - _id: EvBe doi: 10.15479/AT-ISTA-20362 doi_confirm: '1' file: - access_level: open_access checksum: 5ecf274281a54a41e0288bc79edf7492 content_type: application/pdf creator: dbabic date_created: 2025-09-24T13:43:14Z date_updated: 2026-09-25T22:30:02Z embargo: 2026-09-25 file_id: '20388' file_name: 2025_David_Babic_Thesis.pdf file_size: 7501548 relation: main_file - access_level: closed checksum: 2703e548390de0a1af7a707137e8ab3b content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: dbabic date_created: 2025-09-24T13:43:14Z date_updated: 2026-09-25T22:30:02Z embargo_to: open_access file_id: '20389' file_name: Thesis_Babic_draft.docx file_size: 23206052 relation: source_file file_date_updated: 2026-09-25T22:30:02Z fulldoi: https://doi.org/10.15479/AT-ISTA-20362 has_accepted_license: '1' language: - iso: eng month: '09' oa: 1 oa_version: Published Version page: '116' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20187' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 title: Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher _id: '20364' abstract: - lang: eng text: "Auxin is a central regulator of plant growth and development, orchestrating processes from \r\nembryogenesis to senescence through a complex network of biosynthesis, transport, and \r\nsignaling pathways. \r\nIn Arabidopsis thaliana, canonical nuclear auxin signaling is mediated by the TIR1/AFB \r\nreceptors, which promote degradation of AUX/IAA repressors to enable ARF transcription \r\nfactors-driven transcriptional reprogramming. Parallel to this well-established nuclear \r\nmechanism, auxin also triggers rapid, non-transcriptional responses. In particular, at the cell \r\nsurface, most notably through ABP1 and TMKs. Despite decades of research, the integration \r\nof these pathways and their links to fundamental cellular processes remains not fully \r\nunderstood. \r\nIn this study, we uncover a new role for TIR1/AFB signaling in rapidly activating \r\nmacroautophagy during developmental reprogramming. Auxin induces autophagy via \r\nTIR1/AFB adenylate cyclase activity, linking auxin perception to cAMP production and \r\ntranscriptional activation of autophagy machinery. Autophagy-deficient mutants exhibit \r\ndelayed meristem differentiation, impaired lateral root progression, and reduced shoot \r\norganogenesis, demonstrating its necessity for timely developmental transitions. \r\nWe also revisit the ABP1-TMK1 cell-surface auxin perception complex, providing \r\nbiochemical and mass photometry evidence for auxin-dependent complex formation. We show \r\nthat ABP1-TMK1 triggers an ultrafast global phosphorylation response targeting ~1000 \r\nproteins, including the auxin transporter PIN2, and Rho-of-plants (ROP) GTPases regulators. \r\nThis pathway directly regulates PIN2 phosphorylation, for gravitropism and auxin dependent \r\nROP activation. \r\nThese findings establish an integrated framework in which nuclear TIR1/AFB signaling and \r\ncell-surface ABP1-TMK signaling act in parallel to synchronize gene expression, cytoplasmic \r\nremodelling, and transporter regulation, thereby ensuring robust developmental plasticity in \r\nArabidopsis thaliana. " acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: "Plant Facility,\r\nProtein Service Facility" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Caterina full_name: Giannini, Caterina id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 last_name: Giannini citation: ama: Giannini C. Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. 2025. doi:10.15479/AT-ISTA-20364 apa: Giannini, C. (2025). Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20364 chicago: Giannini, Caterina. “Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20364. ieee: C. Giannini, “Nuclear and cell surface auxin signaling in A. thaliana developmental transitions,” Institute of Science and Technology Austria, 2025. ista: Giannini C. 2025. Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. Institute of Science and Technology Austria. mla: Giannini, Caterina. Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20364. short: C. Giannini, Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-09-19T12:23:38Z date_published: 2025-09-19T00:00:00Z date_updated: 2026-10-16T08:24:09Z day: '19' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr - _id: MaLo doi: 10.15479/AT-ISTA-20364 doi_confirm: '1' file: - access_level: open_access checksum: 536ba1701453b0b2346be14c046b2911 content_type: application/pdf creator: cgiannin date_created: 2025-09-24T14:46:34Z date_updated: 2026-09-30T22:30:03Z embargo: 2026-09-30 file_id: '20390' file_name: 2025_Giannini_Caterina_Thesis...pdf file_size: 14278965 relation: main_file - access_level: closed checksum: 192f55262f2da2ea0a59d9c23a1b8573 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: cgiannin date_created: 2025-09-24T14:46:35Z date_updated: 2026-09-30T22:30:03Z embargo_to: open_access file_id: '20391' file_name: 2025_Giannini_Caterina_Thesis...docx file_size: 24499022 relation: source_file file_date_updated: 2026-09-30T22:30:03Z fulldoi: https://doi.org/10.15479/AT-ISTA-20364 has_accepted_license: '1' keyword: - Auxin Signaling - Plant Development language: - iso: eng month: '09' oa: 1 oa_version: Published Version page: '151' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12291' relation: part_of_dissertation status: public - id: '19399' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 title: Nuclear and cell surface auxin signaling in A. thaliana developmental transitions type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: repository OA_type: green _id: '19399' abstract: - lang: eng text: Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a key prerequisite for (i) self-organizing processes including vascular tissue formation and (ii) directional growth responses such as gravitropism. Here we identify a mechanism, by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface ABP1-TMK1 receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE auxin receptor ABL3. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably also for other self-organizing developmental phenomena. acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: 'We thank W. Gray for providing material; N. Gnyliukh and E. Cervenova for help with manuscript preparation; J. Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at ISTA for their excellent service and assistance. The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F; National Natural Science Foundation of China (Grant 32130010, 31422008), start-up funds from FAFU to T.X., Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985- 2016). S.T. was supported by the National Natural Science Foundation of China (32321001).' article_processing_charge: No author: - first_name: Lesia full_name: Rodriguez Solovey, Lesia id: 3922B506-F248-11E8-B48F-1D18A9856A87 last_name: Rodriguez Solovey orcid: 0000-0002-7244-7237 - first_name: Lukas full_name: Fiedler, Lukas id: 7c417475-8972-11ed-ae7b-8b674ca26986 last_name: Fiedler - first_name: Minxia full_name: Zou, Minxia id: 5c243f41-03f3-11ec-841c-96faf48a7ef9 last_name: Zou - first_name: Caterina full_name: Giannini, Caterina id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 last_name: Giannini - first_name: Aline full_name: Monzer, Aline id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425 last_name: Monzer - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Marek full_name: Randuch, Marek id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae last_name: Randuch - first_name: Yongfan full_name: Yu, Yongfan last_name: Yu - first_name: Zuzana full_name: Gelová, Zuzana id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425 last_name: Gelová orcid: 0000-0003-4783-1752 - first_name: Inge full_name: Verstraeten, Inge id: 362BF7FE-F248-11E8-B48F-1D18A9856A87 last_name: Verstraeten orcid: 0000-0001-7241-2328 - first_name: Jakub full_name: Hajny, Jakub id: 4800CC20-F248-11E8-B48F-1D18A9856A87 last_name: Hajny orcid: 0000-0003-2140-7195 - first_name: Meng full_name: Chen, Meng last_name: Chen - first_name: Shutang full_name: Tan, Shutang id: 2DE75584-F248-11E8-B48F-1D18A9856A87 last_name: Tan orcid: 0000-0002-0471-8285 - first_name: Lukas full_name: Hörmayer, Lukas id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87 last_name: Hörmayer orcid: 0000-0001-8295-2926 - first_name: Lanxin full_name: Li, Lanxin id: 367EF8FA-F248-11E8-B48F-1D18A9856A87 last_name: Li orcid: 0000-0002-5607-272X - first_name: Maria Mar full_name: Marques-Bueno, Maria Mar last_name: Marques-Bueno - first_name: Zainab full_name: Quddoos, Zainab id: 32ff3c64-04a0-11f0-a50f-d0c45bfac466 last_name: Quddoos - first_name: Gergely full_name: Molnar, Gergely id: 34F1AF46-F248-11E8-B48F-1D18A9856A87 last_name: Molnar - first_name: Tongda full_name: Xu, Tongda last_name: Xu - first_name: Ivan full_name: Kulich, Ivan id: 57a1567c-8314-11eb-9063-c9ddc3451a54 last_name: Kulich - first_name: Yvon full_name: Jaillais, Yvon last_name: Jaillais - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv. doi:10.1101/2022.11.30.518503 apa: Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (n.d.). ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv. https://doi.org/10.1101/2022.11.30.518503 chicago: Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” BioRxiv, n.d. https://doi.org/10.1101/2022.11.30.518503. ieee: L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism,” bioRxiv. . ista: Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Xu T, Kulich I, Jaillais Y, Friml J. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv, 10.1101/2022.11.30.518503. mla: Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” BioRxiv, doi:10.1101/2022.11.30.518503. short: L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, T. Xu, I. Kulich, Y. Jaillais, J. Friml, BioRxiv (n.d.). corr_author: '1' das_tickbox: '1' date_created: 2025-03-13T08:36:48Z date_published: 2025-02-20T00:00:00Z date_updated: 2026-10-26T23:31:08Z day: '20' ddc: - '580' department: - _id: JiFr - _id: XiFe doi: 10.1101/2022.11.30.518503 ec_funded: 1 fulldoi: https://doi.org/10.1101/2022.11.30.518503 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/2022.11.30.518503 month: '02' oa: 1 oa_version: Published Version project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 26538374-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: I03630 name: Molecular mechanisms of endocytic cargo recognition in plants - _id: 25681D80-B435-11E9-9278-68D0E5697425 call_identifier: FP7 grant_number: '291734' name: International IST Postdoc Fellowship Programme - _id: 26060676-B435-11E9-9278-68D0E5697425 grant_number: ALTF 985-2016 name: Cell surface receptor complexes for auxin signaling in plants publication: bioRxiv publication_status: draft related_material: record: - id: '19395' relation: dissertation_contains status: public - id: '20656' relation: later_version status: public - id: '20364' relation: dissertation_contains status: public status: public title: ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism type: preprint user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- OA_place: publisher _id: '19478' abstract: - lang: eng text: "Auxin is one of the most important phytohormones, playing a crucial role in plant growth and \r\ndevelopment. Genetic studies in Arabidopsis have identified core components of auxin \r\nsignalling and established the classical transcriptional regulation mechanism. This classical \r\nparadigm recognizes TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN \r\nSIGNALLING F-BOX (AFB) proteins as auxin receptors possessing E3 ubiquitin ligase \r\nactivity. Auxin functions as a \"molecular glue\" enhancing TIR1/AFB-Aux/IAA \r\n(AUXIN/INDOLE-3-ACETIC ACID) inhibitor interactions, leading to Aux/IAAs’ \r\nubiquitination and degradation. This process releases AUXIN RESPONSE FACTORs (ARFs) \r\nfrom repression, enabling transcriptional activation of auxin-responsive genes. Notably, the \r\ndiscovery of adenylate cyclase (AC) activity in TIR1/AFBs, catalysing cAMP production from \r\nATP, presents novel transcriptional regulatory possibilities, though cAMP's mechanistic role \r\nin auxin signalling remains unclear. Beyond transcriptional regulation (slow response) a non\r\ntranscriptional pathway (rapid response) mediated by AFB1 also exists, as auxin triggers rapid \r\nroot growth inhibition within 30 seconds. How TIR1 and AFB1, with their distinct subcellular \r\nlocalizations, differentially regulate transcriptional and non-transcriptional processes remains \r\nunclear. To address these two key questions, this study conducted the following investigations. \r\nTIR1 and AFB1 display distinct subcellular localization patterns, with AFB1 specifically \r\nmediating auxin-induced rapid root growth inhibition. To examine whether subcellular \r\nlocalization differences contribute to functional divergence between TIR1 and AFB1, we \r\nutilized microfluidic systems combined with genetic complementation assays. Nuclear export \r\nsignal-fused TIR1 failed to rescue the rapid response defects observed in afb1 mutants. In \r\ncontrast, nuclear localization signal-fused AFB1 exhibited dual functional deficiencies: it \r\nneither restored the rapid response impairment in afb1 mutants nor compensated for \r\ntranscriptional regulation defects in tir1 mutants which limitation stemmed from AFB1's \r\nintrinsic incapacity to assemble functional SCF complexes. These results collectively establish \r\nthat the functional specialization of TIR1 and AFB1 is governed by both their subcellular \r\ncompartmentalization and inherent biochemical characteristics. \r\nThe mechanism by which cAMP derived from the auxin receptor TIR1/AFB modulates \r\ntranscriptional regulation remains undefined. To address this, we genetically dissociated TIR1 \r\nAC activity from its E3 ubiquitin ligase function to investigate cAMP's signalling role. AC\r\ninactivating mutations preserved TIR1-mediated Aux/IAAs’ degradation but abolished ARF\r\ni \r\ndependent transcriptional activation. tir1afb2 mutants expressing AC-deficient TIR1 variants \r\nfailed to restore transcriptional responses. Crucially, compartmentalized cAMP accumulation \r\nwithin Aux/IAA - ARF complexes vicinity triggered auxin signalling independently of both \r\nauxin perception and Aux/IAAs’ degradation, confirming its secondary messenger identity. \r\nFurther mechanistic analysis demonstrated cAMP directly interact with ARF. cAMP \r\ndirectly promotes ARF dissociation from Aux/IAA repressors and enhances ARF \r\nhomodimerization. Genetic complementation assays revealed that ARF mutants lacking \r\nconserved cAMP-binding motifs exhibited partial functional recovery, providing direct \r\nevidence for cAMP-ARF physical interaction during signal propagation. \r\nThese findings collectively challenge the established model of auxin signalling and define \r\na novel TIR1-cAMP-ARF regulatory axis, substantially advancing our comprehension of \r\nauxin-mediated physiological regulation. " acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: "This project was funded by the European Research Council Advanced Grant (ETAP-742985),\r\nEuropean Research Council (ERC; 101142681 CYNIPS), Austrian Science Fund (FWF; P\r\n37051-B)." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Huihuang full_name: Chen, Huihuang id: 83c96512-15b2-11ec-abd3-b7eede36184f last_name: Chen citation: ama: Chen H. The cAMP second messenger in auxin signalling. 2025. doi:10.15479/AT-ISTA-19478 apa: Chen, H. (2025). The cAMP second messenger in auxin signalling. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19478 chicago: Chen, Huihuang. “The CAMP Second Messenger in Auxin Signalling.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19478. ieee: H. Chen, “The cAMP second messenger in auxin signalling,” Institute of Science and Technology Austria, 2025. ista: Chen H. 2025. The cAMP second messenger in auxin signalling. Institute of Science and Technology Austria. mla: Chen, Huihuang. The CAMP Second Messenger in Auxin Signalling. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19478. short: H. Chen, The CAMP Second Messenger in Auxin Signalling, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-04-04T07:48:24Z date_published: 2025-04-04T00:00:00Z date_updated: 2026-10-23T07:25:54Z day: '04' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr doi: 10.15479/AT-ISTA-19478 doi_confirm: '1' ec_funded: 1 file: - access_level: closed checksum: b154973663a1bba505683faab7ae5ead content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: hchen date_created: 2025-04-08T08:00:07Z date_updated: 2026-10-08T22:30:02Z embargo_to: open_access file_id: '19526' file_name: Thesis_0403_Huihuang.docx file_size: 16344814 relation: source_file - access_level: open_access checksum: 0099565f024388830c125ec17375c1a0 content_type: application/pdf creator: hchen date_created: 2025-04-08T08:00:06Z date_updated: 2026-10-09T22:30:03Z embargo: 2026-10-08 file_id: '19527' file_name: Thesis_0406_PDFA_Huihuang_1.pdf file_size: 8482147 relation: main_file file_date_updated: 2026-10-09T22:30:03Z fulldoi: https://doi.org/10.15479/AT-ISTA-19478 has_accepted_license: '1' language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: '118' project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19421' relation: part_of_dissertation status: public - id: '13212' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 title: The cAMP second messenger in auxin signalling type: dissertation user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '19421' abstract: - lang: eng text: The phytohormone auxin (Aux) is a principal endogenous developmental signal in plants. It mediates transcriptional reprogramming by a well-established canonical signalling mechanism. TIR1/AFB auxin receptors are F-box subunits of an ubiquitin ligase complex; after auxin perception, they associate with Aux/IAA transcriptional repressors and ubiquitinate them for degradation, thus enabling the activation of auxin response factor (ARF) transcription factors1,2,3. Here we revise this paradigm by showing that without TIR1 adenylate cyclase (AC) activity4, auxin-induced degradation of Aux/IAAs is not sufficient to mediate the transcriptional auxin response. Abolishing the TIR1 AC activity does not affect auxin-induced degradation of Aux/IAAs but renders TIR1 non-functional in mediating transcriptional reprogramming and auxin-regulated development, including shoot, root, root hair growth and lateral root formation. Transgenic plants show that local cAMP production in the vicinity of the Aux/IAA–ARF complex by unrelated AC enzymes bypasses the need for auxin perception and is sufficient to induce ARF-mediated transcription. These discoveries revise the canonical model of auxin signalling and establish TIR1/AFB-produced cAMP as a second messenger essential for transcriptional reprograming. acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: We are grateful to J. Callis and H.-Q. Yang for sharing materials and to M. Estelle and S. Kepinski for inspiring discussions. This research was supported by the Laboratory Support Facility, the Plant Facility and the Imaging and Optics Facility of the Institute of Science and Technology Austria. This project has received funding from the European Research Council (101142681 CYNIPS) and Austrian Science Fund (P 37051-B). L.Q. was supported by the National Natural Science Foundation of China (grant no. 32470327). M.Z. was supported by the Interdisciplinary Project Committee of the Institute of Science and Technology Austria, and Y.P. was supported by an EMBO Postdoctoral Fellowship (ALTF 38-2023). Open access funding provided by Institute of Science and Technology (IST Austria). article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Huihuang full_name: Chen, Huihuang id: 83c96512-15b2-11ec-abd3-b7eede36184f last_name: Chen - first_name: Linlin full_name: Qi, Linlin id: 44B04502-A9ED-11E9-B6FC-583AE6697425 last_name: Qi orcid: 0000-0001-5187-8401 - first_name: Minxia full_name: Zou, Minxia id: 5c243f41-03f3-11ec-841c-96faf48a7ef9 last_name: Zou - first_name: Mengting full_name: Lu, Mengting id: a8198a14-1ffe-11ee-8b67-d2bdff9d9178 last_name: Lu - first_name: M full_name: Kwiatkowski, M last_name: Kwiatkowski - first_name: Yuanrong full_name: Pei, Yuanrong id: 98605edc-6ce7-11ee-95f3-cc16b866efcd last_name: Pei - first_name: K full_name: Jaworski, K last_name: Jaworski - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Chen H, Qi L, Zou M, et al. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 2025;640:1011-1016. doi:10.1038/s41586-025-08669-w apa: Chen, H., Qi, L., Zou, M., Lu, M., Kwiatkowski, M., Pei, Y., … Friml, J. (2025). TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08669-w chicago: Chen, Huihuang, Linlin Qi, Minxia Zou, Mengting Lu, M Kwiatkowski, Yuanrong Pei, K Jaworski, and Jiří Friml. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-08669-w. ieee: H. Chen et al., “TIR1-produced cAMP as a second messenger in transcriptional auxin signalling,” Nature, vol. 640. Springer Nature, pp. 1011–1016, 2025. ista: Chen H, Qi L, Zou M, Lu M, Kwiatkowski M, Pei Y, Jaworski K, Friml J. 2025. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 640, 1011–1016. mla: Chen, Huihuang, et al. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” Nature, vol. 640, Springer Nature, 2025, pp. 1011–16, doi:10.1038/s41586-025-08669-w. short: H. Chen, L. Qi, M. Zou, M. Lu, M. Kwiatkowski, Y. Pei, K. Jaworski, J. Friml, Nature 640 (2025) 1011–1016. corr_author: '1' date_created: 2025-03-19T09:44:39Z date_published: 2025-04-24T00:00:00Z date_updated: 2026-10-26T23:31:11Z day: '24' ddc: - '580' department: - _id: JiFr doi: 10.1038/s41586-025-08669-w external_id: isi: - '001437493900001' pmid: - '40044868' file: - access_level: open_access checksum: f5f18081003e7a1b8e372ecb7da82e7d content_type: application/pdf creator: dernst date_created: 2025-08-05T12:29:35Z date_updated: 2025-08-05T12:29:35Z file_id: '20132' file_name: 2025_Nature_Chen.pdf file_size: 13549245 relation: main_file success: 1 file_date_updated: 2025-08-05T12:29:35Z fulldoi: https://doi.org/10.1038/s41586-025-08669-w has_accepted_license: '1' intvolume: ' 640' isi: 1 language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: 1011-1016 pmid: 1 project: - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/updating-the-textbook/ record: - id: '19478' relation: dissertation_contains status: public status: public title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 640 year: '2025' ... --- OA_place: publisher OA_type: hybrid _id: '17141' abstract: - lang: eng text: The TIR1/AFB–Aux/IAA–ARF canonical auxin signaling pathway is widely accepted to (de)active transcriptional regulation, thus controlling auxin-associated developmental processes. However, the theme of a rapid auxin response has emerged since the 2018 Auxins and Cytokinin in Plant Development conference. To date, a few signaling components have been identified to mediate both slow and rapid auxin responses, which unveils the complexity of auxin signaling. acknowledgement: "We thank other lab members for their critical comments on this manuscript. We also thank the editor and reviewers for their constructive comments to improve our manuscript. We apologize to authors whose important work we could not include due to space limitations.\r\nThis work is supported by funding from Jiangxi Agricultural University (9232308314) and the Science and Technology Department of Jiangxi Province (20223BCJ25037) to HBH, and the Science and Technology Department of Jiangxi Province (20202ACB215002) to SYP." article_number: erae246 article_processing_charge: No article_type: original author: - first_name: Zilin full_name: Zhang, Zilin last_name: Zhang - first_name: Huihuang full_name: Chen, Huihuang id: 83c96512-15b2-11ec-abd3-b7eede36184f last_name: Chen - first_name: Shuaiying full_name: Peng, Shuaiying last_name: Peng - first_name: Huibin full_name: Han, Huibin last_name: Han citation: ama: Zhang Z, Chen H, Peng S, Han H. Slow and rapid auxin responses in Arabidopsis. Journal of Experimental Botany. 2024;75(18). doi:10.1093/jxb/erae246 apa: Zhang, Z., Chen, H., Peng, S., & Han, H. (2024). Slow and rapid auxin responses in Arabidopsis. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erae246 chicago: Zhang, Zilin, Huihuang Chen, Shuaiying Peng, and Huibin Han. “Slow and Rapid Auxin Responses in Arabidopsis.” Journal of Experimental Botany. Oxford University Press, 2024. https://doi.org/10.1093/jxb/erae246. ieee: Z. Zhang, H. Chen, S. Peng, and H. Han, “Slow and rapid auxin responses in Arabidopsis,” Journal of Experimental Botany, vol. 75, no. 18. Oxford University Press, 2024. ista: Zhang Z, Chen H, Peng S, Han H. 2024. Slow and rapid auxin responses in Arabidopsis. Journal of Experimental Botany. 75(18), erae246. mla: Zhang, Zilin, et al. “Slow and Rapid Auxin Responses in Arabidopsis.” Journal of Experimental Botany, vol. 75, no. 18, erae246, Oxford University Press, 2024, doi:10.1093/jxb/erae246. short: Z. Zhang, H. Chen, S. Peng, H. Han, Journal of Experimental Botany 75 (2024). das_tickbox: '0' date_created: 2024-06-15T19:50:15Z date_published: 2024-09-27T00:00:00Z date_updated: 2026-10-02T11:25:38Z day: '27' ddc: - '580' department: - _id: GradSch - _id: JiFr doi: 10.1093/jxb/erae246 external_id: isi: - '001270051200001' pmid: - '38794966' file: - access_level: open_access checksum: 91b9435ed0f6640809c7588df19abf2f content_type: application/pdf creator: dernst date_created: 2025-01-02T10:26:22Z date_updated: 2025-01-02T10:26:22Z file_id: '18720' file_name: 2024_JourExperimentalBotany_Zhang.pdf file_size: 763097 relation: main_file success: 1 file_date_updated: 2025-01-02T10:26:22Z fulldoi: https://doi.org/10.1093/jxb/erae246 has_accepted_license: '1' intvolume: ' 75' isi: 1 issue: '18' language: - iso: eng month: '09' oa: 1 oa_version: Published Version pmid: 1 publication: Journal of Experimental Botany publication_identifier: issn: - 0022-0957 publication_status: published publisher: Oxford University Press quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: Slow and rapid auxin responses in Arabidopsis tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 75 year: '2024' ... --- OA_place: repository _id: '18689' abstract: - lang: eng text: Multiplexed fluorescence microscopy imaging is widely used in biomedical applications. However, simultaneous imaging of multiple fluorophores can result in spectral leaks and overlapping, which greatly degrades image quality and subsequent analysis. Existing popular spectral unmixing methods are mainly based on computational intensive linear models and the performance is heavily dependent on the reference spectra, which may greatly preclude its further applications. In this paper, we propose a deep learning-based blindly spectral unmixing method, termed AutoUnmix, to imitate the physical spectral mixing process. A tranfer learning framework is further devised to allow our AutoUnmix adapting to a variety of imaging systems without retraining the network. Our proposed method has demonstrated real-time unmixing capabilities, surpassing existing methods by up to 100-fold in terms of unmixing speed. We further validate the reconstruction performance on both synthetic datasets and biological samples. The unmixing results of AutoUnmix achieve a highest SSIM of 0.99 in both three- and four-color imaging, with nearly up to 20% higher than other popular unmixing methods. Due to the desirable property of data independency and superior blind unmixing performance, we believe AutoUnmix is a powerful tool to study the interaction process of different organelles labeled by multiple fluorophores. acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: M-Shop - _id: E-Lib acknowledgement: "We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\n\r\nThis project has received funding from the Austrian Science Fund (FWF): I 3630-B25 (J.G.D) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 742985, J.F.). It has also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385. S.T. has received funding as an ISTplus Fellow from the European Union’s Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from an EMBO Long-Term Fellowship (grant number ALTF 679-2018). It has further received funding from the Austrian Science Fund (FWF) grant DK W1232 (M.T, N.A-D., J.G.D). W.J. received funding via a Human Frontier Science Program postdoctoral fellowship LT000557/2018.\r\n\r\nThe funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript." article_processing_charge: No author: - first_name: Michelle C full_name: Gallei, Michelle C id: 35A03822-F248-11E8-B48F-1D18A9856A87 last_name: Gallei orcid: 0000-0003-1286-7368 - first_name: Sven M full_name: Truckenbrodt, Sven M id: 45812BD4-F248-11E8-B48F-1D18A9856A87 last_name: Truckenbrodt - first_name: Caroline full_name: Kreuzinger, Caroline id: 382077BA-F248-11E8-B48F-1D18A9856A87 last_name: Kreuzinger - first_name: Syamala full_name: Inumella, Syamala id: F8660870-D756-11E9-98C5-34DFE5697425 last_name: Inumella orcid: 0009-0002-5890-120X - first_name: Vitali full_name: Vistunou, Vitali id: 7e146587-8972-11ed-ae7b-d7a32ea86a81 last_name: Vistunou - first_name: Christoph M full_name: Sommer, Christoph M id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87 last_name: Sommer orcid: 0000-0003-1216-9105 - first_name: Mojtaba full_name: Tavakoli, Mojtaba id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87 last_name: Tavakoli orcid: 0000-0002-7667-6854 - first_name: Nathalie full_name: Agudelo Duenas, Nathalie id: 40E7F008-F248-11E8-B48F-1D18A9856A87 last_name: Agudelo Duenas - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 - first_name: Wiebke full_name: Jahr, Wiebke id: 425C1CE8-F248-11E8-B48F-1D18A9856A87 last_name: Jahr orcid: 0000-0003-0201-2315 - first_name: Marek full_name: Randuch, Marek id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae last_name: Randuch - first_name: Alexander J full_name: Johnson, Alexander J id: 46A62C3A-F248-11E8-B48F-1D18A9856A87 last_name: Johnson orcid: 0000-0002-2739-8843 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 citation: ama: Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. bioRxiv. doi:10.1101/2024.02.21.581330 apa: Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (n.d.). Super-resolution expansion microscopy in plant roots. bioRxiv. https://doi.org/10.1101/2024.02.21.581330 chicago: Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” BioRxiv, n.d. https://doi.org/10.1101/2024.02.21.581330. ieee: M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” bioRxiv. . ista: Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. Super-resolution expansion microscopy in plant roots. bioRxiv, 10.1101/2024.02.21.581330. mla: Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” BioRxiv, doi:10.1101/2024.02.21.581330. short: M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, BioRxiv (n.d.). corr_author: '1' das_tickbox: '0' date_created: 2024-12-19T12:28:00Z date_published: 2024-02-21T00:00:00Z date_updated: 2026-10-02T11:27:43Z day: '21' department: - _id: EvBe - _id: JoDa - _id: JiFr doi: 10.1101/2024.02.21.581330 ec_funded: 1 fulldoi: https://doi.org/10.1101/2024.02.21.581330 language: - iso: eng license: https://creativecommons.org/licenses/by-nc/4.0/ main_file_link: - open_access: '1' url: https://doi.org/10.1101/2024.02.21.581330 month: '02' oa: 1 oa_version: Preprint project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships - _id: 26AA4EF2-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232-B24 name: Molecular Drug Targets - _id: 269B5B22-B435-11E9-9278-68D0E5697425 grant_number: ALTF 679-2018 name: UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED publication: bioRxiv publication_status: draft related_material: record: - id: '19003' relation: later_version status: public - id: '18681' relation: dissertation_contains status: public researchdata_availability: no status: public supplementarymaterial: no title: Super-resolution expansion microscopy in plant roots tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: preprint user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 year: '2024' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '14251' abstract: - lang: eng text: The phytohormone auxin and its directional transport through tissues play a fundamental role in development of higher plants. This polar auxin transport predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization is crucial for development, but its evolution during the rise of morphological complexity in land plants remains unclear. Here, we performed a cross-species investigation by observing the trafficking and localization of endogenous and exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha, and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion did not compromise the auxin export function of all examined PINs by using radioactive auxin export assay and by observing the phenotypic changes in transgenic bryophytes. Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs distribute symmetrically on the membrane in both bryophytes. In Arabidopsis root epidermis, bryophytic PINs show no defined polarity. Pharmacological interference revealed a strong cytoskeleton dependence of bryophytic but not Arabidopsis PIN polarization. The divergence of PIN polarization and trafficking is also observed within the bryophyte clade and between tissues of individual species. These results collectively reveal a divergence of PIN trafficking and polarity mechanisms throughout land plant evolution and a co-evolution of PIN sequence-based and cell-based polarity mechanisms. acknowledgement: This work was supported by the ERC grant (PR1023ERC02) to H. T. and J. F., and by the ministry of science and technology (grant number 110-2636-B-005-001) to K. J. L. article_number: '100669' article_processing_charge: Yes article_type: original author: - first_name: Han full_name: Tang, Han id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E last_name: Tang orcid: 0000-0001-6152-6637 - first_name: KJ full_name: Lu, KJ last_name: Lu - first_name: Y full_name: Zhang, Y last_name: Zhang - first_name: YL full_name: Cheng, YL last_name: Cheng - first_name: SL full_name: Tu, SL last_name: Tu - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution. Plant Communications. 2024;5(1). doi:10.1016/j.xplc.2023.100669 apa: Tang, H., Lu, K., Zhang, Y., Cheng, Y., Tu, S., & Friml, J. (2024). Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2023.100669 chicago: Tang, Han, KJ Lu, Y Zhang, YL Cheng, SL Tu, and Jiří Friml. “Divergence of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land Plant Evolution.” Plant Communications. Elsevier, 2024. https://doi.org/10.1016/j.xplc.2023.100669. ieee: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, and J. Friml, “Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution,” Plant Communications, vol. 5, no. 1. Elsevier, 2024. ista: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. 2024. Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution. Plant Communications. 5(1), 100669. mla: Tang, Han, et al. “Divergence of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land Plant Evolution.” Plant Communications, vol. 5, no. 1, 100669, Elsevier, 2024, doi:10.1016/j.xplc.2023.100669. short: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, J. Friml, Plant Communications 5 (2024). corr_author: '1' das_tickbox: '0' date_created: 2023-09-01T11:32:02Z date_published: 2024-01-08T00:00:00Z date_updated: 2026-10-02T12:23:32Z day: '08' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.xplc.2023.100669 ec_funded: 1 external_id: isi: - '001158054500001' pmid: - '37528584' file: - access_level: open_access checksum: edbc44c6d4a394d2bf70f92fdbb08f0a content_type: application/pdf creator: dernst date_created: 2024-01-30T12:59:57Z date_updated: 2024-01-30T12:59:57Z file_id: '14911' file_name: 2023_PlantCommunications_Tang.pdf file_size: 2825565 relation: main_file success: 1 file_date_updated: 2024-01-30T12:59:57Z fulldoi: https://doi.org/10.1016/j.xplc.2023.100669 has_accepted_license: '1' intvolume: ' 5' isi: 1 issue: '1' language: - iso: eng month: '01' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants publication: Plant Communications publication_identifier: issn: - 2590-3462 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: yes title: Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 5 year: '2024' ... --- _id: '14447' abstract: - lang: eng text: "Auxin belongs among major phytohormones and governs multiple aspects of plant growth and development. The establishment of auxin concentration gradients, determines, among other processes, plant organ positioning and growth responses to environmental stimuli.\r\nHerein we report the synthesis of new NBD- or DNS-labelled IAA derivatives and the elucidation of their biological activity, fluorescence properties and subcellular accumulation patterns in planta. These novel compounds did not show auxin-like activity, but instead antagonized physiological auxin effects. The DNS-labelled derivatives FL5 and FL6 showed strong anti-auxin activity in roots and hypocotyls, which also occurred at the level of gene transcription as confirmed by quantitative PCR analysis. The auxin antagonism of our derivatives was further demonstrated in vitro using an SPR-based binding assay. The NBD-labelled compound FL4 with the best fluorescence properties proved to be unsuitable to study auxin accumulation patterns in planta. On the other hand, the strongest anti-auxin activity possessing compounds FL5 and FL6 could be useful to study binding mechanisms to auxin receptors and for manipulations of auxin-regulated processes." acknowledgement: The authors would like to thank Karolína Kubiasová and Iñigo Saiz-Fernández for valuable scientific discussions. Open access publishing supported by the National Technical Library in Prague. This work was supported by the Palacký University Olomouc Young Researcher Grant Competition (JG_2020_002), by the Internal Grant Agency of Palacký University Olomouc (IGA_PrF_2023_016, IGA_PrF_2023_031), by the Ministry of Education, Youth and Sports of the Czech Republic through the European Regional Development Fund-Project Plants as a tool for sustainable global development (CZ.02.1.01/0.0/0.0/16_019/0000827) and the project Support of mobility at Palacký University Olomouc II. (CZ.02.2.69/0.0/0.0/18_053/0016919). The Biacore T200 SPR instrument was provided by the WISB Research Technology Facility within the School of Life Sciences, University of Warwick. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Kristýna full_name: Bieleszová, Kristýna last_name: Bieleszová - first_name: Pavel full_name: Hladík, Pavel last_name: Hladík - first_name: Martin full_name: Kubala, Martin last_name: Kubala - first_name: Richard full_name: Napier, Richard last_name: Napier - first_name: Federica full_name: Brunoni, Federica last_name: Brunoni - first_name: Zuzana full_name: Gelová, Zuzana id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425 last_name: Gelová orcid: 0000-0003-4783-1752 - first_name: Lukas full_name: Fiedler, Lukas id: 7c417475-8972-11ed-ae7b-8b674ca26986 last_name: Fiedler - first_name: Ivan full_name: Kulich, Ivan id: 57a1567c-8314-11eb-9063-c9ddc3451a54 last_name: Kulich - first_name: Miroslav full_name: Strnad, Miroslav last_name: Strnad - first_name: Karel full_name: Doležal, Karel last_name: Doležal - first_name: Ondřej full_name: Novák, Ondřej last_name: Novák - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Asta full_name: Žukauskaitė, Asta last_name: Žukauskaitė citation: ama: 'Bieleszová K, Hladík P, Kubala M, et al. New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis thaliana. Plant Growth Regulation. 2024;102:589-602. doi:10.1007/s10725-023-01083-0' apa: 'Bieleszová, K., Hladík, P., Kubala, M., Napier, R., Brunoni, F., Gelová, Z., … Žukauskaitė, A. (2024). New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis thaliana. Plant Growth Regulation. Springer Nature. https://doi.org/10.1007/s10725-023-01083-0' chicago: 'Bieleszová, Kristýna, Pavel Hladík, Martin Kubala, Richard Napier, Federica Brunoni, Zuzana Gelová, Lukas Fiedler, et al. “New Fluorescent Auxin Derivatives: Anti-Auxin Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant Growth Regulation. Springer Nature, 2024. https://doi.org/10.1007/s10725-023-01083-0.' ieee: 'K. Bieleszová et al., “New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis thaliana,” Plant Growth Regulation, vol. 102. Springer Nature, pp. 589–602, 2024.' ista: 'Bieleszová K, Hladík P, Kubala M, Napier R, Brunoni F, Gelová Z, Fiedler L, Kulich I, Strnad M, Doležal K, Novák O, Friml J, Žukauskaitė A. 2024. New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis thaliana. Plant Growth Regulation. 102, 589–602.' mla: 'Bieleszová, Kristýna, et al. “New Fluorescent Auxin Derivatives: Anti-Auxin Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant Growth Regulation, vol. 102, Springer Nature, 2024, pp. 589–602, doi:10.1007/s10725-023-01083-0.' short: K. Bieleszová, P. Hladík, M. Kubala, R. Napier, F. Brunoni, Z. Gelová, L. Fiedler, I. Kulich, M. Strnad, K. Doležal, O. Novák, J. Friml, A. Žukauskaitė, Plant Growth Regulation 102 (2024) 589–602. das_tickbox: '1' dataavailabilitystatement: The data that supports the findings of this study are available from the corresponding authors upon reasonable request. date_created: 2023-10-22T22:01:15Z date_published: 2024-04-01T00:00:00Z date_updated: 2026-10-02T12:24:54Z day: '01' ddc: - '580' department: - _id: JiFr doi: 10.1007/s10725-023-01083-0 external_id: isi: - '001084334300001' file: - access_level: open_access checksum: e63271b1d57c1f03c1d993fd62cba59c content_type: application/pdf creator: dernst date_created: 2024-07-16T08:13:24Z date_updated: 2024-07-16T08:13:24Z file_id: '17252' file_name: 2024_PlantGrowthReg_Bieleszova.pdf file_size: 2847929 relation: main_file success: 1 file_date_updated: 2024-07-16T08:13:24Z fulldoi: https://doi.org/10.1007/s10725-023-01083-0 has_accepted_license: '1' intvolume: ' 102' isi: 1 language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: 589-602 publication: Plant Growth Regulation publication_identifier: eissn: - 1573-5087 issn: - 0167-6903 publication_status: published publisher: Springer Nature quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: 'New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis thaliana' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 102 year: '2024' ... --- DOAJ_listed: '1' _id: '17048' abstract: - lang: eng text: A key mechanism employed by plants to adapt to salinity stress involves maintaining ion homeostasis via the actions of ion transporters. While the function of cation transporters in maintaining ion homeostasis in plants has been extensively studied, little is known about the roles of their anion counterparts in this process. Here, we describe a mechanism of salt adaptation in plants. We characterized the chloride channel (CLC) gene AtCLCf, whose expression is regulated by WRKY transcription factor under salt stress in Arabidopsis thaliana. Loss-of-function atclcf seedlings show increased sensitivity to salt, whereas AtCLCf overexpression confers enhanced resistance to salt stress. Salt stress induces the translocation of GFP-AtCLCf fusion protein to the plasma membrane (PM). Blocking AtCLCf translocation using the exocytosis inhibitor brefeldin-A or mutating the small GTPase gene AtRABA1b/BEX5 (RAS GENES FROM RAT BRAINA1b homolog) increases salt sensitivity in plants. Electrophysiology and liposome-based assays confirm the Cl−/H+ antiport function of AtCLCf. Therefore, we have uncovered a mechanism of plant adaptation to salt stress involving the NaCl-induced translocation of AtCLCf to the PM, thus facilitating Cl− removal at the roots, and increasing the plant’s salinity tolerance. acknowledgement: The authors thank Drs. Akihiko Nakano and Tomohiro Uemura (RIKEN and Ochanomizu University, Japan) for providing plant material (seeds of GFP-RABA1bQ72L GFP-RABA1bS27N), Dr. Prakash Arumugam (SIFBI, A*STAR, Singapore) for providing the yeast strains used in this study, and Dr. Jobichen Chacko for help with homology model building. We thank Prof. Elliot Meyerowitz (Caltech) and Dr. On Sun Lau (NUS) for critical reading of our manuscript. The National University of Singapore provided partial financial support as grant number A−8000149-03-00, and PhD research scholarship to S.R. article_number: '3978' article_processing_charge: Yes article_type: original author: - first_name: Sivamathini full_name: Rajappa, Sivamathini last_name: Rajappa - first_name: Pannaga full_name: Krishnamurthy, Pannaga last_name: Krishnamurthy - first_name: Hua full_name: Huang, Hua last_name: Huang - first_name: Dejie full_name: Yu, Dejie last_name: Yu - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Jian full_name: Xu, Jian last_name: Xu - first_name: Prakash P. full_name: Kumar, Prakash P. last_name: Kumar citation: ama: Rajappa S, Krishnamurthy P, Huang H, et al. The translocation of a chloride channel from the Golgi to the plasma membrane helps plants adapt to salt stress. Nature Communications. 2024;15. doi:10.1038/s41467-024-48234-z apa: Rajappa, S., Krishnamurthy, P., Huang, H., Yu, D., Friml, J., Xu, J., & Kumar, P. P. (2024). The translocation of a chloride channel from the Golgi to the plasma membrane helps plants adapt to salt stress. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-48234-z chicago: Rajappa, Sivamathini, Pannaga Krishnamurthy, Hua Huang, Dejie Yu, Jiří Friml, Jian Xu, and Prakash P. Kumar. “The Translocation of a Chloride Channel from the Golgi to the Plasma Membrane Helps Plants Adapt to Salt Stress.” Nature Communications. Springer Nature, 2024. https://doi.org/10.1038/s41467-024-48234-z. ieee: S. Rajappa et al., “The translocation of a chloride channel from the Golgi to the plasma membrane helps plants adapt to salt stress,” Nature Communications, vol. 15. Springer Nature, 2024. ista: Rajappa S, Krishnamurthy P, Huang H, Yu D, Friml J, Xu J, Kumar PP. 2024. The translocation of a chloride channel from the Golgi to the plasma membrane helps plants adapt to salt stress. Nature Communications. 15, 3978. mla: Rajappa, Sivamathini, et al. “The Translocation of a Chloride Channel from the Golgi to the Plasma Membrane Helps Plants Adapt to Salt Stress.” Nature Communications, vol. 15, 3978, Springer Nature, 2024, doi:10.1038/s41467-024-48234-z. short: S. Rajappa, P. Krishnamurthy, H. Huang, D. Yu, J. Friml, J. Xu, P.P. Kumar, Nature Communications 15 (2024). das_tickbox: '1' dataavailabilitystatement: All data are available in the main text or the supplementary materials. Source data are provided with this paper. date_created: 2024-05-26T22:00:57Z date_published: 2024-05-10T00:00:00Z date_updated: 2026-10-02T12:26:55Z day: '10' ddc: - '580' department: - _id: JiFr doi: 10.1038/s41467-024-48234-z external_id: isi: - '001221549300004' pmid: - '38729926' file: - access_level: open_access checksum: 79aacbe31cf7626b78da062b1339bdb0 content_type: application/pdf creator: dernst date_created: 2024-05-27T07:43:46Z date_updated: 2024-05-27T07:43:46Z file_id: '17056' file_name: 2024_NatureComm_Rajappa.pdf file_size: 20961818 relation: main_file success: 1 file_date_updated: 2024-05-27T07:43:46Z fulldoi: https://doi.org/10.1038/s41467-024-48234-z has_accepted_license: '1' intvolume: ' 15' isi: 1 language: - iso: eng month: '05' oa: 1 oa_version: Published Version pmid: 1 publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' researchdata_availability: yes (supplementary data) scopus_import: '1' status: public supplementarymaterial: yes title: The translocation of a chloride channel from the Golgi to the plasma membrane helps plants adapt to salt stress tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 15 year: '2024' ... --- OA_place: repository OA_type: green _id: '22776' article_processing_charge: No author: - first_name: Yunlong full_name: Du, Yunlong last_name: Du citation: ama: Du Y. SeRrbJ609. 2024. doi:10.6084/m9.figshare.25448191 apa: Du, Y. (2024). SeRrbJ609. Repository. https://doi.org/10.6084/m9.figshare.25448191 chicago: Du, Yunlong. “SeRrbJ609.” Repository, 2024. https://doi.org/10.6084/m9.figshare.25448191. ieee: Y. Du, “SeRrbJ609.” Repository, 2024. ista: Du Y. 2024. SeRrbJ609, Repository, 10.6084/m9.figshare.25448191. mla: Du, Yunlong. SeRrbJ609. Repository, 2024, doi:10.6084/m9.figshare.25448191. short: Y. Du, (2024). date_created: 2026-09-03T09:17:46Z date_published: 2024-03-21T00:00:00Z date_updated: 2026-10-02T12:31:36Z day: '21' ddc: - '580' department: - _id: JiFr doi: 10.6084/m9.figshare.25448191 fulldoi: https://doi.org/10.6084/m9.figshare.25448191 main_file_link: - open_access: '1' url: https://doi.org/10.6084/m9.figshare.25448191 month: '03' oa: 1 oa_version: None publisher: Repository related_material: record: - id: '17103' relation: research_data status: public status: public title: SeRrbJ609 type: research_data_reference user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 year: '2024' ... --- DOAJ_listed: '1' _id: '17103' abstract: - lang: eng text: Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription. acknowledgement: We thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese Academy of Sciences) for his generous support with the sakuranetin measurement. We thank International Rice Research Institute (IRRI) for provision of the rice NILs. We thank Professor Zhongkai Zhang (Yunnan Academy of Agricultural Sciences) for his generous support with subcellular structure observation of rice roots. We thank Professor Barbara Valent (Kansas State University) for her generously provision of the plasmid containing the PWL2 gene. We thank Professor Zuhua He (Chinese Academy of Sciences) for the gift of the transgenic rice line expressing the Pigm gene, OsNPR1-RNAi mutant line and ROD1-overexpression rice line. We thank Professor Yinong Yang (The Pennsylvania State University) for provision of the rice line NahG. We thank Professor Muyuan Zhu (Zhejiang University) for provision of the transgenic plants overexpressing miR393a. We thank Professor Zhengge Zhu (Hebei Normal University) for provision of the rice line overexpressing OsPIN3t-GFP. We thank Professor Yanhua Qi (Zhejiang University) for provision of the arf12 mutant line. Thanks also go to Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision of the fungus M. oryzae strain Guy11 (AvrCo39-mRFP). This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 32260085, 31460453, 31660501, 31860064, 31760500 and 31901870), the Major Special Program for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005). The project was also sponsored by SRF for ROCS, SEM (Grant No. [2013] 1792), the Key Projects of Applied Basic Research Plan of Yunnan Province (Grant No. 2017FA018, 202301AS070082), the Major Science and Technology Project in Yunnan Province (202102AE090042, 202202AE090036 and 202102AE090017), the Young and Middle-Aged Academic and Technical Leaders Reserve Talent Program in Yunnan Province (202205AC160076), the China Postdoctoral Science Foundation (2019M653849XB) and the National Key Research and Development Program of China (2023YFE0107500). article_number: '3437' article_processing_charge: Yes article_type: original author: - first_name: Lihui full_name: Jiang, Lihui last_name: Jiang - first_name: Xiaoyan full_name: Zhang, Xiaoyan last_name: Zhang - first_name: Yiting full_name: Zhao, Yiting last_name: Zhao - first_name: Haiyan full_name: Zhu, Haiyan last_name: Zhu - first_name: Qijing full_name: Fu, Qijing last_name: Fu - first_name: Xinqi full_name: Lu, Xinqi last_name: Lu - first_name: Wuying full_name: Huang, Wuying last_name: Huang - first_name: Xinyue full_name: Yang, Xinyue last_name: Yang - first_name: Xuan full_name: Zhou, Xuan last_name: Zhou - first_name: Lixia full_name: Wu, Lixia last_name: Wu - first_name: Ao full_name: Yang, Ao last_name: Yang - first_name: Xie full_name: He, Xie last_name: He - first_name: Man full_name: Dong, Man last_name: Dong - first_name: Ziai full_name: Peng, Ziai last_name: Peng - first_name: Jing full_name: Yang, Jing last_name: Yang - first_name: Liwei full_name: Guo, Liwei last_name: Guo - first_name: Jiancheng full_name: Wen, Jiancheng last_name: Wen - first_name: Huichuan full_name: Huang, Huichuan last_name: Huang - first_name: Yong full_name: Xie, Yong last_name: Xie - first_name: Shusheng full_name: Zhu, Shusheng last_name: Zhu - first_name: Chengyun full_name: Li, Chengyun last_name: Li - first_name: Xiahong full_name: He, Xiahong last_name: He - first_name: Youyong full_name: Zhu, Youyong last_name: Zhu - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Yunlong full_name: Du, Yunlong last_name: Du citation: ama: Jiang L, Zhang X, Zhao Y, et al. Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast. Nature Communications. 2024;15. doi:10.1038/s41467-024-47746-y apa: Jiang, L., Zhang, X., Zhao, Y., Zhu, H., Fu, Q., Lu, X., … Du, Y. (2024). Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-47746-y chicago: Jiang, Lihui, Xiaoyan Zhang, Yiting Zhao, Haiyan Zhu, Qijing Fu, Xinqi Lu, Wuying Huang, et al. “Phytoalexin Sakuranetin Attenuates Endocytosis and Enhances Resistance to Rice Blast.” Nature Communications. Springer Nature, 2024. https://doi.org/10.1038/s41467-024-47746-y. ieee: L. Jiang et al., “Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast,” Nature Communications, vol. 15. Springer Nature, 2024. ista: Jiang L, Zhang X, Zhao Y, Zhu H, Fu Q, Lu X, Huang W, Yang X, Zhou X, Wu L, Yang A, He X, Dong M, Peng Z, Yang J, Guo L, Wen J, Huang H, Xie Y, Zhu S, Li C, He X, Zhu Y, Friml J, Du Y. 2024. Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast. Nature Communications. 15, 3437. mla: Jiang, Lihui, et al. “Phytoalexin Sakuranetin Attenuates Endocytosis and Enhances Resistance to Rice Blast.” Nature Communications, vol. 15, 3437, Springer Nature, 2024, doi:10.1038/s41467-024-47746-y. short: L. Jiang, X. Zhang, Y. Zhao, H. Zhu, Q. Fu, X. Lu, W. Huang, X. Yang, X. Zhou, L. Wu, A. Yang, X. He, M. Dong, Z. Peng, J. Yang, L. Guo, J. Wen, H. Huang, Y. Xie, S. Zhu, C. Li, X. He, Y. Zhu, J. Friml, Y. Du, Nature Communications 15 (2024). das_tickbox: '1' dataavailabilitystatement: All data supporting the findings of this study are available within the paper, its Supplementary Information file, and the Source Data file. The LC-MS/MS data and microscopy data have been deposited at the public repository figshare with the dataset identifier SeRrbJ609 [https://doi.org/10.6084/m9.figshare.25448191.v3]. Original data points in graphs and qPCR data are shown in the Source Data files. Source data are provided with this paper. date_created: 2024-06-03T08:54:50Z date_published: 2024-04-23T00:00:00Z date_updated: 2026-10-02T12:32:10Z day: '23' ddc: - '580' department: - _id: JiFr doi: 10.1038/s41467-024-47746-y external_id: isi: - '001207290500013' pmid: - '38653755' file: - access_level: open_access checksum: 80cb2f2c538e81064f4836b54bc313ca content_type: application/pdf creator: dernst date_created: 2024-06-03T12:05:10Z date_updated: 2024-06-03T12:05:10Z file_id: '17110' file_name: 2024_NatureComm_Jiang.pdf file_size: 8013695 relation: main_file success: 1 file_date_updated: 2024-06-03T12:05:10Z fulldoi: https://doi.org/10.1038/s41467-024-47746-y has_accepted_license: '1' intvolume: ' 15' isi: 1 language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: record: - id: '22776' relation: used_in_publication status: public - id: '23039' relation: research_data status: public researchdata_availability: yes (supplementary data) scopus_import: '1' status: public supplementarymaterial: yes title: Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 15 year: '2024' ... --- APC_amount: 2792,52 EUR DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '15033' abstract: - lang: eng text: The GNOM (GN) Guanine nucleotide Exchange Factor for ARF small GTPases (ARF-GEF) is among the best studied trafficking regulators in plants, playing crucial and unique developmental roles in patterning and polarity. The current models place GN at the Golgi apparatus (GA), where it mediates secretion/recycling, and at the plasma membrane (PM) presumably contributing to clathrin-mediated endocytosis (CME). The mechanistic basis of the developmental function of GN, distinct from the other ARF-GEFs including its closest homologue GNOM-LIKE1 (GNL1), remains elusive. Insights from this study largely extend the current notions of GN function. We show that GN, but not GNL1, localizes to the cell periphery at long-lived structures distinct from clathrin-coated pits, while CME and secretion proceed normally in gn knockouts. The functional GN mutant variant GNfewerroots, absent from the GA, suggests that the cell periphery is the major site of GN action responsible for its developmental function. Following inhibition by Brefeldin A, GN, but not GNL1, relocates to the PM likely on exocytic vesicles, suggesting selective molecular associations en route to the cell periphery. A study of GN-GNL1 chimeric ARF-GEFs indicates that all GN domains contribute to the specific GN function in a partially redundant manner. Together, this study offers significant steps toward the elucidation of the mechanism underlying unique cellular and development functions of GNOM. acknowledgement: The authors would like to gratefully acknowledge Dr Xixi Zhang for cloning the GNL1/pDONR221 construct and for useful discussions.H2020 European Research Council Advanced Grant ETAP742985 to Jiří Friml, Austrian Science Fund I 3630-B25 to Jiří Friml article_processing_charge: Yes article_type: original author: - first_name: Maciek full_name: Adamowski, Maciek id: 45F536D2-F248-11E8-B48F-1D18A9856A87 last_name: Adamowski orcid: 0000-0001-6463-5257 - first_name: Ivana full_name: Matijevic, Ivana id: 83c17ce3-15b2-11ec-abd3-f486545870bd last_name: Matijevic - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Adamowski M, Matijevic I, Friml J. Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery. eLife. 2024;13. doi:10.7554/elife.68993 apa: Adamowski, M., Matijevic, I., & Friml, J. (2024). Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.68993 chicago: Adamowski, Maciek, Ivana Matijevic, and Jiří Friml. “Developmental Patterning Function of GNOM ARF-GEF Mediated from the Cell Periphery.” ELife. eLife Sciences Publications, 2024. https://doi.org/10.7554/elife.68993. ieee: M. Adamowski, I. Matijevic, and J. Friml, “Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery,” eLife, vol. 13. eLife Sciences Publications, 2024. ista: Adamowski M, Matijevic I, Friml J. 2024. Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery. eLife. 13. mla: Adamowski, Maciek, et al. “Developmental Patterning Function of GNOM ARF-GEF Mediated from the Cell Periphery.” ELife, vol. 13, eLife Sciences Publications, 2024, doi:10.7554/elife.68993. short: M. Adamowski, I. Matijevic, J. Friml, ELife 13 (2024). corr_author: '1' das_tickbox: '1' dataavailabilitystatement: All data generated or analysed during this study are included in the manuscript and supporting files. No additional source files were provided. date_created: 2024-02-27T07:10:11Z date_published: 2024-02-21T00:00:00Z date_updated: 2026-10-15T08:07:21Z day: '21' ddc: - '580' department: - _id: JiFr doi: 10.7554/elife.68993 ec_funded: 1 external_id: isi: - '001174278000001' pmid: - '38381485' file: - access_level: open_access checksum: b2b2d583b433823af731842f1420113e content_type: application/pdf creator: dernst date_created: 2024-07-22T11:51:50Z date_updated: 2024-07-22T11:51:50Z file_id: '17310' file_name: 2024_eLife_Adamowski.pdf file_size: 15675744 relation: main_file success: 1 file_date_updated: 2024-07-22T11:51:50Z fulldoi: https://doi.org/10.7554/elife.68993 has_accepted_license: '1' intvolume: ' 13' isi: 1 keyword: - General Immunology and Microbiology - General Biochemistry - Genetics and Molecular Biology - General Medicine - General Neuroscience language: - iso: eng month: '02' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 26538374-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: I03630 name: Molecular mechanisms of endocytic cargo recognition in plants - _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1 call_identifier: FWF name: FWF Open Access Fund publication: eLife publication_identifier: issn: - 2050-084X publication_status: published publisher: eLife Sciences Publications quality_controlled: '1' researchdata_availability: yes (supplementary data) scopus_import: '1' status: public supplementarymaterial: no title: Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 13 year: '2024' ... --- _id: '14826' abstract: - lang: eng text: The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that this response occurs across 5 land plant and algal species and converges on a core group of shared targets. We found conserved rapid physiological responses to auxin in the same species and identified rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation across species. Genetic analysis connects this kinase to both auxin-triggered protein phosphorylation and rapid cellular response, thus identifying an ancient mechanism for fast auxin responses in the green lineage. acknowledgement: 'We are grateful to Asuka Shitaku and Eri Koide for generating and sharing the Marchantia PRAF-mCitrine line and Peng-Cheng Wang for sharing the Arabidopsis raf mutant. We are grateful to our team members for discussions and helpful advice. This work was supported by funding from the Netherlands Organization for Scientific Research (NWO): VICI grant 865.14.001 and ENW-KLEIN OCENW.KLEIN.027 grants to D.W.; VENI grant VI.VENI.212.003 to A.K.; the European Research Council AdG DIRNDL (contract number 833867) to D.W.; CoG CATCH to J.S.; StG CELLONGATE (contract 803048) to M.F.; and AdG ETAP (contract 742985) to J.F.; MEXT KAKENHI grant number JP19H05675 to T.K.; JSPS KAKENHI grant number JP20H03275 to R.N.; Takeda Science Foundation to R.N.; and the Austrian Science Fund (FWF, P29988) to J.F.' article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Andre full_name: Kuhn, Andre last_name: Kuhn - first_name: Mark full_name: Roosjen, Mark last_name: Roosjen - first_name: Sumanth full_name: Mutte, Sumanth last_name: Mutte - first_name: Shiv Mani full_name: Dubey, Shiv Mani last_name: Dubey - first_name: Vanessa Polet full_name: Carrillo Carrasco, Vanessa Polet last_name: Carrillo Carrasco - first_name: Sjef full_name: Boeren, Sjef last_name: Boeren - first_name: Aline full_name: Monzer, Aline id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425 last_name: Monzer - first_name: Jasper full_name: Koehorst, Jasper last_name: Koehorst - first_name: Takayuki full_name: Kohchi, Takayuki last_name: Kohchi - first_name: Ryuichi full_name: Nishihama, Ryuichi last_name: Nishihama - first_name: Matyas full_name: Fendrych, Matyas id: 43905548-F248-11E8-B48F-1D18A9856A87 last_name: Fendrych orcid: 0000-0002-9767-8699 - first_name: Joris full_name: Sprakel, Joris last_name: Sprakel - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Dolf full_name: Weijers, Dolf last_name: Weijers citation: ama: Kuhn A, Roosjen M, Mutte S, et al. RAF-like protein kinases mediate a deeply conserved, rapid auxin response. Cell. 2024;187(1):130-148.e17. doi:10.1016/j.cell.2023.11.021 apa: Kuhn, A., Roosjen, M., Mutte, S., Dubey, S. M., Carrillo Carrasco, V. P., Boeren, S., … Weijers, D. (2024). RAF-like protein kinases mediate a deeply conserved, rapid auxin response. Cell. Elsevier. https://doi.org/10.1016/j.cell.2023.11.021 chicago: Kuhn, Andre, Mark Roosjen, Sumanth Mutte, Shiv Mani Dubey, Vanessa Polet Carrillo Carrasco, Sjef Boeren, Aline Monzer, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid Auxin Response.” Cell. Elsevier, 2024. https://doi.org/10.1016/j.cell.2023.11.021. ieee: A. Kuhn et al., “RAF-like protein kinases mediate a deeply conserved, rapid auxin response,” Cell, vol. 187, no. 1. Elsevier, p. 130–148.e17, 2024. ista: Kuhn A, Roosjen M, Mutte S, Dubey SM, Carrillo Carrasco VP, Boeren S, Monzer A, Koehorst J, Kohchi T, Nishihama R, Fendrych M, Sprakel J, Friml J, Weijers D. 2024. RAF-like protein kinases mediate a deeply conserved, rapid auxin response. Cell. 187(1), 130–148.e17. mla: Kuhn, Andre, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid Auxin Response.” Cell, vol. 187, no. 1, Elsevier, 2024, p. 130–148.e17, doi:10.1016/j.cell.2023.11.021. short: A. Kuhn, M. Roosjen, S. Mutte, S.M. Dubey, V.P. Carrillo Carrasco, S. Boeren, A. Monzer, J. Koehorst, T. Kohchi, R. Nishihama, M. Fendrych, J. Sprakel, J. Friml, D. Weijers, Cell 187 (2024) 130–148.e17. das_tickbox: '1' dataavailabilitystatement: "Phosphoproteome data are available in the ProteomeXchange PRIDE database: PXD038369 and PXD038363. Transcriptome data are available in the NCBI Sequence Read Archive: PRJNA881051. Data underlying RAF-like kinase phylogeny is deposited at the iTOL: https://itol.embl.de/shared/dolfweijers. This paper analyzes existing, publicly available data phospho-proteome data. The accession number for the datasets are listed in the key resources table.\r\n\r\nCode for the AuxPhos app and for MATlab code for analysis of cytoplasmic streaming are available at Github: https://github.com/WeijersLab/AuxPhos and https://github.com/jorissprakel/RAF_track_analysis.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead author upon request." date_created: 2024-01-17T12:45:40Z date_published: 2024-01-04T00:00:00Z date_updated: 2026-10-15T08:06:16Z day: '04' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.cell.2023.11.021 ec_funded: 1 external_id: isi: - '001152705700001' pmid: - '38128538' file: - access_level: open_access checksum: 06fd236a9ee0b46ccb05f44695bfc34b content_type: application/pdf creator: dernst date_created: 2024-01-22T13:41:41Z date_updated: 2024-01-22T13:41:41Z file_id: '14874' file_name: 2024_Cell_Kuhn.pdf file_size: 13194060 relation: main_file success: 1 file_date_updated: 2024-01-22T13:41:41Z fulldoi: https://doi.org/10.1016/j.cell.2023.11.021 has_accepted_license: '1' intvolume: ' 187' isi: 1 issue: '1' keyword: - General Biochemistry - Genetics and Molecular Biology language: - iso: eng month: '01' oa: 1 oa_version: Published Version page: 130-148.e17 pmid: 1 project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 262EF96E-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: P29988 name: RNA-directed DNA methylation in plant development publication: Cell publication_identifier: eissn: - 1097-4172 issn: - 0092-8674 publication_status: published publisher: Elsevier quality_controlled: '1' related_material: record: - id: '19395' relation: dissertation_contains status: public researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: RAF-like protein kinases mediate a deeply conserved, rapid auxin response tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 187 year: '2024' ... --- _id: '15319' abstract: - lang: eng text: Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and Fourier transform infrared spectroscopic analyses corroborated deficiencies in the composition of trichome cell walls in these mutants. Transgenic lines expressing fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant. Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo triple mutant plants. In vivo protein–protein interaction assays in plant and yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed synergistically enhanced resistance to powdery mildew attack. Taken together, our data point to an isoform-specific interplay of EXO70 and MLO proteins in the modulation of trichome cell wall biogenesis and powdery mildew susceptibility. article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Jan W. full_name: Huebbers, Jan W. last_name: Huebbers - first_name: George A. full_name: Caldarescu, George A. last_name: Caldarescu - first_name: Zdeňka full_name: Kubátová, Zdeňka last_name: Kubátová - first_name: Peter full_name: Sabol, Peter last_name: Sabol - first_name: Sophie C.J. full_name: Levecque, Sophie C.J. last_name: Levecque - first_name: Hannah full_name: Kuhn, Hannah last_name: Kuhn - first_name: Ivan full_name: Kulich, Ivan id: 57a1567c-8314-11eb-9063-c9ddc3451a54 last_name: Kulich - first_name: Anja full_name: Reinstädler, Anja last_name: Reinstädler - first_name: Kim full_name: Büttgen, Kim last_name: Büttgen - first_name: Alba full_name: Manga-Robles, Alba last_name: Manga-Robles - first_name: Hugo full_name: Mélida, Hugo last_name: Mélida - first_name: Markus full_name: Pauly, Markus last_name: Pauly - first_name: Ralph full_name: Panstruga, Ralph last_name: Panstruga - first_name: Viktor full_name: Žárský, Viktor last_name: Žárský citation: ama: Huebbers JW, Caldarescu GA, Kubátová Z, et al. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. 2024;36(4):1007-1035. doi:10.1093/plcell/koad319 apa: Huebbers, J. W., Caldarescu, G. A., Kubátová, Z., Sabol, P., Levecque, S. C. J., Kuhn, H., … Žárský, V. (2024). Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koad319 chicago: Huebbers, Jan W., George A. Caldarescu, Zdeňka Kubátová, Peter Sabol, Sophie C.J. Levecque, Hannah Kuhn, Ivan Kulich, et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” Plant Cell. Oxford University Press, 2024. https://doi.org/10.1093/plcell/koad319. ieee: J. W. Huebbers et al., “Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew,” Plant Cell, vol. 36, no. 4. Oxford University Press, pp. 1007–1035, 2024. ista: Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský V. 2024. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. 36(4), 1007–1035. mla: Huebbers, Jan W., et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” Plant Cell, vol. 36, no. 4, Oxford University Press, 2024, pp. 1007–35, doi:10.1093/plcell/koad319. short: J.W. Huebbers, G.A. Caldarescu, Z. Kubátová, P. Sabol, S.C.J. Levecque, H. Kuhn, I. Kulich, A. Reinstädler, K. Büttgen, A. Manga-Robles, H. Mélida, M. Pauly, R. Panstruga, V. Žárský, Plant Cell 36 (2024) 1007–1035. das_tickbox: '1' dataavailabilitystatement: All relevant data can be found within the manuscript and its supporting materials. date_created: 2024-04-14T22:01:02Z date_published: 2024-04-01T00:00:00Z date_updated: 2026-10-15T08:09:23Z day: '01' ddc: - '580' department: - _id: JiFr doi: 10.1093/plcell/koad319 external_id: pmid: - '38124479' file: - access_level: open_access checksum: f9994d2e4748feec24c992b39871aba1 content_type: application/pdf creator: dernst date_created: 2024-04-17T06:57:54Z date_updated: 2024-04-17T06:57:54Z file_id: '15326' file_name: 2024_PlantCell_Huebbers.pdf file_size: 2866098 relation: main_file success: 1 file_date_updated: 2024-04-17T06:57:54Z fulldoi: https://doi.org/10.1093/plcell/koad319 has_accepted_license: '1' intvolume: ' 36' issue: '4' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '04' oa: 1 oa_version: Published Version page: 1007-1035 pmid: 1 publication: Plant Cell publication_identifier: eissn: - 1532-298X issn: - 1040-4651 publication_status: published publisher: Oxford University Press quality_controlled: '1' researchdata_availability: yes (supplementary data) scopus_import: '1' status: public supplementarymaterial: yes title: Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 36 year: '2024' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '17436' abstract: - lang: eng text: The auxin signaling molecule controls a variety of growth and developmental processes in land plants. Auxin regulates gene expression through a nuclear auxin signaling pathway (NAP) consisting of the ubiquitin ligase auxin receptor TIR1/AFB, its Aux/IAA degradation substrate, and DNA-binding ARF transcription factors. Although extensive qualitative understanding of the pathway and its interactions has been obtained, mostly by studying the flowering plant Arabidopsis thaliana, it remains unknown how these translate to quantitative system behavior in vivo, a problem that is confounded by the large NAP gene families in most species. Here, we used the minimal NAP of the liverwort Marchantia polymorpha to quantitatively map NAP protein accumulation and dynamics in vivo through the use of knockin fluorescent fusion proteins. Beyond revealing the dynamic native accumulation profile of the entire NAP protein network, we discovered that the two central ARFs, MpARF1 and MpARF2, are proteasomally degraded. This auxin-independent degradation tunes ARF protein stoichiometry to favor gene activation, thereby reprogramming auxin response during the developmental progression. Thus, quantitative analysis of the entire NAP has enabled us to identify ARF degradation and the stoichiometries of activator and repressor ARFs as a potential mechanism for controlling gemma germination. acknowledgement: "We are grateful to Iris Nieuwland and Neri van Laar for experimental support. No conflict of interest declared.\r\nThis work was supported by the Netherlands Organisation for Scientific Research, the Netherlands (grants ALWOP.402 and OCENW.M20.031 to J.W.B.) and the Human Frontiers Research Program (grant RGP0015/2022 to D.W.)." article_number: '101039' article_processing_charge: Yes article_type: original author: - first_name: Shubhajit full_name: Das, Shubhajit id: b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be last_name: Das - first_name: Martijn full_name: De Roij, Martijn last_name: De Roij - first_name: Simon full_name: Bellows, Simon last_name: Bellows - first_name: Melissa Dipp full_name: Alvarez, Melissa Dipp last_name: Alvarez - first_name: Sumanth full_name: Mutte, Sumanth last_name: Mutte - first_name: Wouter full_name: Kohlen, Wouter last_name: Kohlen - first_name: Etienne full_name: Farcot, Etienne last_name: Farcot - first_name: Dolf full_name: Weijers, Dolf last_name: Weijers - first_name: Jan Willem full_name: Borst, Jan Willem last_name: Borst citation: ama: Das S, De Roij M, Bellows S, et al. Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. Plant Communications. 2024;5(11). doi:10.1016/j.xplc.2024.101039 apa: Das, S., De Roij, M., Bellows, S., Alvarez, M. D., Mutte, S., Kohlen, W., … Borst, J. W. (2024). Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101039 chicago: Das, Shubhajit, Martijn De Roij, Simon Bellows, Melissa Dipp Alvarez, Sumanth Mutte, Wouter Kohlen, Etienne Farcot, Dolf Weijers, and Jan Willem Borst. “Quantitative Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.” Plant Communications. Elsevier, 2024. https://doi.org/10.1016/j.xplc.2024.101039. ieee: S. Das et al., “Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination,” Plant Communications, vol. 5, no. 11. Elsevier, 2024. ista: Das S, De Roij M, Bellows S, Alvarez MD, Mutte S, Kohlen W, Farcot E, Weijers D, Borst JW. 2024. Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. Plant Communications. 5(11), 101039. mla: Das, Shubhajit, et al. “Quantitative Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.” Plant Communications, vol. 5, no. 11, 101039, Elsevier, 2024, doi:10.1016/j.xplc.2024.101039. short: S. Das, M. De Roij, S. Bellows, M.D. Alvarez, S. Mutte, W. Kohlen, E. Farcot, D. Weijers, J.W. Borst, Plant Communications 5 (2024). das_tickbox: '0' date_created: 2024-08-18T22:01:04Z date_published: 2024-11-11T00:00:00Z date_updated: 2026-10-15T08:12:01Z day: '11' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.xplc.2024.101039 external_id: pmid: - '38988072' file: - access_level: open_access checksum: 38cabc1042ac7fb70e6c4c510eba88fc content_type: application/pdf creator: dernst date_created: 2025-01-09T12:25:32Z date_updated: 2025-01-09T12:25:32Z file_id: '18811' file_name: 2024_PlantCommunications_Das.pdf file_size: 4970540 relation: main_file success: 1 file_date_updated: 2025-01-09T12:25:32Z fulldoi: https://doi.org/10.1016/j.xplc.2024.101039 has_accepted_license: '1' intvolume: ' 5' issue: '11' language: - iso: eng month: '11' oa: 1 oa_version: Published Version pmid: 1 publication: Plant Communications publication_identifier: eissn: - 2590-3462 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: yes title: Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 5 year: '2024' ... --- OA_place: publisher OA_type: hybrid _id: '18465' abstract: - lang: eng text: The phytohormone auxin is polarly transported in plants by PIN-FORMED (PIN) transporters and controls virtually all growth and developmental processes. Canonical PINs possess a long, largely disordered cytosolic loop. Auxin transport by canonical PINs is activated by loop phosphorylation by certain kinases. The structure of the PIN transmembrane domains was recently determined, their transport properties remained poorly characterized, and the role of the loop in the transport process was unclear. Here, we determined the quantitative kinetic parameters of auxin transport mediated by Arabidopsis PINs to mathematically model auxin distribution in roots and to test these predictions in vivo. Using chimeras between transmembrane and loop domains of different PINs, we demonstrate a strong correlation between transport parameters and physiological output, indicating that the loop domain is not only required to activate PIN-mediated auxin transport, but it has an additional role in the transport process by a currently unknown mechanism. acknowledgement: This work was funded by DFG3468/6-1, DFG3468/6-3, and SFB924 to U.Z.H. We thank Angela Alkofer and Helene Prunkl for excellent technical assistance and Xenopus maintenance. Christian Luschnig is acknowledged for sharing unpublished results and valuable discussions. article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: DP full_name: Janacek, DP last_name: Janacek - first_name: M full_name: Kolb, M last_name: Kolb - first_name: L full_name: Schulz, L last_name: Schulz - first_name: J full_name: Mergner, J last_name: Mergner - first_name: B full_name: Kuster, B last_name: Kuster - first_name: Matous full_name: Glanc, Matous id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2 last_name: Glanc orcid: 0000-0003-0619-7783 - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: K full_name: Ten Tusscher, K last_name: Ten Tusscher - first_name: C full_name: Schwechheimer, C last_name: Schwechheimer - first_name: UZ full_name: Hammes, UZ last_name: Hammes citation: ama: Janacek D, Kolb M, Schulz L, et al. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 2024;59(14):S1534-5807(24)00569-0. doi:10.1016/j.devcel.2024.09.020 apa: Janacek, D., Kolb, M., Schulz, L., Mergner, J., Kuster, B., Glanc, M., … Hammes, U. (2024). Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.09.020 chicago: Janacek, DP, M Kolb, L Schulz, J Mergner, B Kuster, Matous Glanc, Jiří Friml, K Ten Tusscher, C Schwechheimer, and UZ Hammes. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” Developmental Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.09.020. ieee: D. Janacek et al., “Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport,” Developmental Cell, vol. 59, no. 14. Elsevier, pp. S1534-5807(24)00569–0, 2024. ista: Janacek D, Kolb M, Schulz L, Mergner J, Kuster B, Glanc M, Friml J, Ten Tusscher K, Schwechheimer C, Hammes U. 2024. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 59(14), S1534-5807(24)00569–0. mla: Janacek, DP, et al. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” Developmental Cell, vol. 59, no. 14, Elsevier, 2024, pp. S1534-5807(24)00569-0, doi:10.1016/j.devcel.2024.09.020. short: D. Janacek, M. Kolb, L. Schulz, J. Mergner, B. Kuster, M. Glanc, J. Friml, K. Ten Tusscher, C. Schwechheimer, U. Hammes, Developmental Cell 59 (2024) S1534-5807(24)00569–0. das_tickbox: '1' dataavailabilitystatement: "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD044850 (user name: reviewer_pxd044850@ebi.ac.uk, password: 3xBdgLSX).59\r\n\r\nThis paper does not report original codes.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request." date_created: 2024-10-23T08:41:27Z date_published: 2024-12-16T00:00:00Z date_updated: 2026-10-15T08:13:11Z day: '16' ddc: - '570' department: - _id: JiFr doi: 10.1016/j.devcel.2024.09.020 external_id: isi: - '001390774300001' pmid: - '39413780' file: - access_level: open_access checksum: 34423ee9fb4e30334f3572eddf1da2ae content_type: application/pdf creator: dernst date_created: 2025-01-13T09:20:15Z date_updated: 2025-01-13T09:20:15Z file_id: '18835' file_name: 2024_DevelopmentalCell_Janacek.pdf file_size: 3675955 relation: main_file success: 1 file_date_updated: 2025-01-13T09:20:15Z fulldoi: https://doi.org/10.1016/j.devcel.2024.09.020 has_accepted_license: '1' intvolume: ' 59' isi: 1 issue: '14' language: - iso: eng month: '12' oa: 1 oa_version: Published Version page: S1534-5807(24)00569-0 pmid: 1 publication: Developmental Cell publication_identifier: eissn: - 1878-1551 issn: - 1534-5807 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 59 year: '2024' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '18582' abstract: - lang: eng text: Identification of PIN exporters for auxin, the major coordinative signal in plants, some 25 years ago, signifies a landmark in our understanding of plant-specific mechanisms underlying development and adaptation. Auxin is directionally transported throughout the plant body; a unique feature already envisioned by Darwin and solidified by PINs’ discovery and characterization. The PIN-based auxin distribution network with its complex regulations of PIN expression, localization and activity turned out to underlie a remarkable multitude of developmental processes and represents means to integrate endogenous and environmental signals. Given the recent anniversary, we here summarize past and current developments in this exciting field. acknowledgement: We gratefully acknowledge Leo Gälweiler for authorizing his PIN1 story. We would like to thank Yuanrong Pei for invaluable help with preparing figures. Work in the lab of C.L. is supported by grants from the Austrian Science Fund (PAT 8419423) and by the Gesellschaft für Forschungsförderung Niederösterreich m.b.H. (FTI19-008). The lab of J.F. is supported by the Austrian Science Fund (I 6123-B and P 37051-B). article_number: '9904' article_processing_charge: Yes article_type: original author: - first_name: Christian full_name: Luschnig, Christian last_name: Luschnig - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Luschnig C, Friml J. Over 25 years of decrypting PIN-mediated plant development. Nature Communications. 2024;15. doi:10.1038/s41467-024-54240-y apa: Luschnig, C., & Friml, J. (2024). Over 25 years of decrypting PIN-mediated plant development. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-54240-y chicago: Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” Nature Communications. Springer Nature, 2024. https://doi.org/10.1038/s41467-024-54240-y. ieee: C. Luschnig and J. Friml, “Over 25 years of decrypting PIN-mediated plant development,” Nature Communications, vol. 15. Springer Nature, 2024. ista: Luschnig C, Friml J. 2024. Over 25 years of decrypting PIN-mediated plant development. Nature Communications. 15, 9904. mla: Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” Nature Communications, vol. 15, 9904, Springer Nature, 2024, doi:10.1038/s41467-024-54240-y. short: C. Luschnig, J. Friml, Nature Communications 15 (2024). corr_author: '1' das_tickbox: '0' date_created: 2024-11-24T23:01:48Z date_published: 2024-12-01T00:00:00Z date_updated: 2026-10-15T08:10:07Z day: '01' ddc: - '580' department: - _id: JiFr doi: 10.1038/s41467-024-54240-y external_id: isi: - '001356232600004' pmid: - '39548100' file: - access_level: open_access checksum: 3a31af06f52100d287f1e9d9c2aa1d40 content_type: application/pdf creator: dernst date_created: 2024-12-03T14:10:54Z date_updated: 2024-12-03T14:10:54Z file_id: '18615' file_name: 2024_NatureComm_Luschnig.pdf file_size: 1426555 relation: main_file success: 1 file_date_updated: 2024-12-03T14:10:54Z fulldoi: https://doi.org/10.1038/s41467-024-54240-y has_accepted_license: '1' intvolume: ' 15' isi: 1 language: - iso: eng month: '12' oa: 1 oa_version: Published Version pmid: 1 project: - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: Over 25 years of decrypting PIN-mediated plant development tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 15 year: '2024' ... --- OA_place: repository OA_type: green _id: '15251' abstract: - lang: eng text: Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling. acknowledgement: "We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support, Y. Yin (Iowa State University, Ames, IA, USA) for providing the anti-BES1 antibody, Y. Gao and all other staff members for their technical support on cryo-EM data collection, S. Vanneste (VIB, Ghent University, Ghent, Belgium) for useful discussions, and M. De Cock for help in preparing the manuscript.\r\nThis work was supported by the National Natural Science Foundation of China (grants 31870732 and 32322041 to L.S., grant 31900885 to X.L., and grant 32321001 to L.S.); the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB37020103 to L.S.); the Natural Science Foundation of Anhui Province (grant 2008085MC90 to X.L. and grant 2008085J15 to L.S.); Fundamental Research Funds for the Central Universities (grant WK9100000031 to L.S.); USTC Research Funds of the Double First-Class Initiative (grant YD9100002004 to L.S. and grant YD9100002020 to X.L.); Research Foundation-Flanders (grant G002121N to E.R. and postdoctoral fellowships 12R7822N and 12R7819N to N.V.); and Chinese Scholarship Council predoctoral fellowships (Y.W. and H.Z.). L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation." article_processing_charge: No article_type: original author: - first_name: Wei full_name: Ying, Wei last_name: Ying - first_name: Yaowei full_name: Wang, Yaowei last_name: Wang - first_name: Hong full_name: Wei, Hong last_name: Wei - first_name: Yongming full_name: Luo, Yongming last_name: Luo - first_name: Qian full_name: Ma, Qian last_name: Ma - first_name: Heyuan full_name: Zhu, Heyuan last_name: Zhu - first_name: Hilde full_name: Janssens, Hilde last_name: Janssens - first_name: Nemanja full_name: Vukašinović, Nemanja last_name: Vukašinović - first_name: Miroslav full_name: Kvasnica, Miroslav last_name: Kvasnica - first_name: Johan M. full_name: Winne, Johan M. last_name: Winne - first_name: Yongxiang full_name: Gao, Yongxiang last_name: Gao - first_name: Shutang full_name: Tan, Shutang id: 2DE75584-F248-11E8-B48F-1D18A9856A87 last_name: Tan orcid: 0000-0002-0471-8285 - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Xin full_name: Liu, Xin last_name: Liu - first_name: Eugenia full_name: Russinova, Eugenia last_name: Russinova - first_name: Linfeng full_name: Sun, Linfeng last_name: Sun citation: ama: Ying W, Wang Y, Wei H, et al. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. Science. 2024;383(6689):eadj4591. doi:10.1126/science.adj4591 apa: Ying, W., Wang, Y., Wei, H., Luo, Y., Ma, Q., Zhu, H., … Sun, L. (2024). Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.adj4591 chicago: Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde Janssens, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” Science. American Association for the Advancement of Science, 2024. https://doi.org/10.1126/science.adj4591. ieee: W. Ying et al., “Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export,” Science, vol. 383, no. 6689. American Association for the Advancement of Science, p. eadj4591, 2024. ista: Ying W, Wang Y, Wei H, Luo Y, Ma Q, Zhu H, Janssens H, Vukašinović N, Kvasnica M, Winne JM, Gao Y, Tan S, Friml J, Liu X, Russinova E, Sun L. 2024. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. Science. 383(6689), eadj4591. mla: Ying, Wei, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” Science, vol. 383, no. 6689, American Association for the Advancement of Science, 2024, p. eadj4591, doi:10.1126/science.adj4591. short: W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović, M. Kvasnica, J.M. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun, Science 383 (2024) eadj4591. das_tickbox: '1' dataavailabilitystatement: All data are available in the main text or the supplementary materials. Model coordinates and cryo-EM density maps for the WT ABCB19 structures in the substrate-unbound state, BL-bound state, BL and AMP-PNP-bound state, and the E529Q/E1174Q mutant ABCB19 structure in the ATP-bound state have been deposited in the Protein Data Bank with accession codes 8WOI, 8WOM, 8WOO, and 8WP0, respectively, and in the Electron Microscopy Data Bank with accession codes EMD-37690, EMD-37692, EMD-37694, and EMD-37705, respectively. date_created: 2024-03-31T22:01:12Z date_published: 2024-03-22T00:00:00Z date_updated: 2026-10-15T08:08:30Z day: '22' department: - _id: JiFr doi: 10.1126/science.adj4591 external_id: isi: - '001252955200028' pmid: - '38513023' fulldoi: https://doi.org/10.1126/science.adj4591 intvolume: ' 383' isi: 1 issue: '6689' language: - iso: eng main_file_link: - open_access: '1' url: http://hdl.handle.net/1854/LU-01HTMBK3P3PPNB73YFCMFXP3ZZ month: '03' oa: 1 oa_version: Submitted Version page: eadj4591 pmid: 1 publication: Science publication_identifier: eissn: - 1095-9203 publication_status: published publisher: American Association for the Advancement of Science quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 383 year: '2024' ...