--- OA_place: repository OA_type: green _id: '21994' abstract: - lang: eng text: Adaptive plant development is orchestrated, among others, by directional, intercellular transport of the phytohormone auxin. Self-organizing development, such as flexible vasculature formation, depends on so-called auxin canalization, manifested by the gradual formation of auxin transport channels through feedback between auxin signalling and transport. Herein, we identify MAKR6 as an important, novel component in this feedback. MAKR6 expression accumulates strongly in vascular cells and is tightly regulated by auxin via the Aux/IAA-ARF-WRKY23 transcriptional network. MAKR6 is required for auxin canalization-dependent processes, including leaf venation, vasculature regeneration, and de novo auxin channel formation from local auxin sources. Mechanistically, MAKR6 interacts with the PIN1 auxin transporter, modulating its trafficking and polarization. MAKR6 also associates with and integrates two key receptor-like kinase complexes involved in canalization, TMK1/4 and the CAMEL-CANAR. Together, our study establishes MAKR6 as a multifaceted regulator that couples transcriptional auxin signalling to PIN1 repolarization and coordinates multiple RLK-mediated signalling pathways during canalization. This provides mechanistic insights into auxin canalization and exemplifies a framework for exploring similar regulatory nodes in other developmental contexts. acknowledgement: 'We would like to thank Dr. Yvon Jaillais (ENS, Lyon) for sharing MAKR2 materials. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The research in the Friml group leading to these results was funded by the European Research Council (ERC): 101142681 CYNIPS; and the Austrian Science Fund (FWF): I 6123-B and P 37051-B. Ewa Mazur was supported by the National Science Centre (NCN), Poland, under the OPUS call in the WEAVE programme: 2021/43/I/NZ1/01835.' article_processing_charge: No author: - first_name: Zengxiang full_name: Ge, Zengxiang id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8 last_name: Ge orcid: 0000-0001-9381-3577 - first_name: Lilla full_name: Koczka, Lilla last_name: Koczka - first_name: Ewa full_name: Mazur, Ewa last_name: Mazur - first_name: Gergely full_name: Molnar, Gergely id: 34F1AF46-F248-11E8-B48F-1D18A9856A87 last_name: Molnar - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Nada full_name: Kassem, Nada last_name: Kassem - first_name: Sara full_name: Ait Ikene, Sara id: 6a0bb896-6bad-11f1-9bef-906e9eb76034 last_name: Ait Ikene - first_name: Lukas full_name: Fiedler, Lukas id: 7c417475-8972-11ed-ae7b-8b674ca26986 last_name: Fiedler - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Ge Z, Koczka L, Mazur E, et al. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv. doi:10.1101/2025.10.07.680881 apa: Ge, Z., Koczka, L., Mazur, E., Molnar, G., Vladimirtsev, D., Kassem, N., … Friml, J. (n.d.). MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv. https://doi.org/10.1101/2025.10.07.680881 chicago: Ge, Zengxiang, Lilla Koczka, Ewa Mazur, Gergely Molnar, Dmitrii Vladimirtsev, Nada Kassem, Sara Ait Ikene, Lukas Fiedler, and Jiří Friml. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.07.680881. ieee: Z. Ge et al., “MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis,” bioRxiv. . ista: Ge Z, Koczka L, Mazur E, Molnar G, Vladimirtsev D, Kassem N, Ait Ikene S, Fiedler L, Friml J. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv, 10.1101/2025.10.07.680881. mla: Ge, Zengxiang, et al. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” BioRxiv, doi:10.1101/2025.10.07.680881. short: Z. Ge, L. Koczka, E. Mazur, G. Molnar, D. Vladimirtsev, N. Kassem, S. Ait Ikene, L. Fiedler, J. Friml, BioRxiv (n.d.). corr_author: '1' date_created: 2026-06-13T16:57:07Z date_published: 2026-05-30T00:00:00Z date_updated: 2026-06-19T07:14:01Z day: '30' ddc: - '580' department: - _id: GradSch - _id: JiFr doi: 10.1101/2025.10.07.680881 has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ main_file_link: - open_access: '1' url: https://doi.org/10.1101/2025.10.07.680881 month: '05' oa: 1 oa_version: Preprint project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: bioRxiv publication_status: submitted scopus_import: '1' status: public title: MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: preprint user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher _id: '20964' acknowledged_ssus: - _id: LifeSc - _id: Bio alternative_title: - ISTA Master’s Thesis article_processing_charge: No author: - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev citation: ama: Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:10.15479/AT-ISTA-20964 apa: Vladimirtsev, D. (2026). Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20964 chicago: Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-20964. ieee: D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026. ista: Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria. mla: Vladimirtsev, Dmitrii. Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-20964. short: D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026. corr_author: '1' date_created: 2026-01-09T09:22:48Z date_published: 2026-01-14T00:00:00Z date_updated: 2026-04-07T11:41:44Z day: '14' ddc: - '570' degree_awarded: MS department: - _id: GradSch - _id: JiFr doi: 10.15479/AT-ISTA-20964 file: - access_level: closed checksum: 812857b2fbe3f6113bef22fd04bccd3e content_type: application/pdf creator: dvladimi date_created: 2026-01-21T14:12:13Z date_updated: 2026-01-21T14:12:13Z embargo: 2027-01-01 embargo_to: open_access file_id: '21033' file_name: 2026_Vladimirtsev_Dmitrii_Thesis.pdf file_size: 2867531 relation: main_file - access_level: closed checksum: 2b969f97f8d7461bea3d255f48c2219c content_type: application/x-zip-compressed creator: dvladimi date_created: 2026-01-21T14:41:58Z date_updated: 2026-01-28T12:38:19Z file_id: '21034' file_name: Source Files.zip file_size: 25023066 relation: source_file file_date_updated: 2026-01-28T12:38:19Z has_accepted_license: '1' language: - iso: eng month: '01' oa_version: Published Version page: '22' project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants publication_identifier: issn: - 2791-4585 publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20982' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 title: Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '21490' abstract: - lang: eng text: Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development. acknowledged_ssus: - _id: MassSpec - _id: Bio - _id: LifeSc acknowledgement: We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Mingyue full_name: Li, Mingyue id: 01f96916-0235-11eb-9379-a323192643b7 last_name: Li - first_name: Nikola full_name: Rydza, Nikola last_name: Rydza - first_name: Ewa full_name: Mazur, Ewa last_name: Mazur - first_name: Gergely full_name: Molnar, Gergely id: 34F1AF46-F248-11E8-B48F-1D18A9856A87 last_name: Molnar - first_name: Tomasz full_name: Nodzyński, Tomasz last_name: Nodzyński - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023 apa: Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023 chicago: Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023. ieee: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026. ista: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6. mla: Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023. short: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6. corr_author: '1' date_created: 2026-03-23T15:11:16Z date_published: 2026-03-23T00:00:00Z date_updated: 2026-03-24T08:36:40Z day: '23' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.cub.2026.02.023 external_id: pmid: - '41831441' file: - access_level: open_access checksum: fe6c41fdab58a55df5f2a5860c02acdc content_type: application/pdf creator: dernst date_created: 2026-03-24T08:34:37Z date_updated: 2026-03-24T08:34:37Z file_id: '21496' file_name: 2026_CurrentBiology_Li.pdf file_size: 12986894 relation: main_file success: 1 file_date_updated: 2026-03-24T08:34:37Z has_accepted_license: '1' intvolume: ' 36' issue: '6' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '03' oa: 1 oa_version: Published Version page: 1468-1480.e6 pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: bd906599-d553-11ed-ba76-abf8547645d7 grant_number: E271 name: Identification of a novel regulator in auxin canalization publication: Current Biology publication_identifier: issn: - 0960-9822 publication_status: published publisher: Elsevier quality_controlled: '1' status: public title: Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 36 year: '2026' ... --- OA_place: repository OA_type: green _id: '21763' abstract: - lang: eng text: Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil. acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: "We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license." article_processing_charge: No article_type: original author: - first_name: Ivan full_name: Kulich, Ivan id: 57a1567c-8314-11eb-9063-c9ddc3451a54 last_name: Kulich - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Marek full_name: Randuch, Marek id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae last_name: Randuch - first_name: Shiqiang full_name: Gao, Shiqiang last_name: Gao - first_name: Matteo full_name: Citterico, Matteo last_name: Citterico - first_name: Kai R. full_name: Konrad, Kai R. last_name: Konrad - first_name: Georg full_name: Nagel, Georg last_name: Nagel - first_name: Michael full_name: Wrzaczek, Michael last_name: Wrzaczek - first_name: Léa full_name: Cascaro, Léa last_name: Cascaro - first_name: Pauline full_name: Vinet, Pauline last_name: Vinet - first_name: Pauline full_name: Durand, Pauline last_name: Durand - first_name: Atef full_name: Asnacios, Atef last_name: Asnacios - first_name: Lokesh full_name: Verma, Lokesh last_name: Verma - first_name: Malcolm J. full_name: Bennett, Malcolm J. last_name: Bennett - first_name: Bipin K. full_name: Pandey, Bipin K. last_name: Pandey - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 2026;392(6795):296-300. doi:10.1126/science.adu8197 apa: Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. AAAS. https://doi.org/10.1126/science.adu8197 chicago: Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” Science. AAAS, 2026. https://doi.org/10.1126/science.adu8197. ieee: I. Kulich et al., “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” Science, vol. 392, no. 6795. AAAS, pp. 296–300, 2026. ista: Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300. mla: Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” Science, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:10.1126/science.adu8197. short: I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300. corr_author: '1' date_created: 2026-04-26T22:01:47Z date_published: 2026-04-16T00:00:00Z date_updated: 2026-05-07T06:20:07Z day: '16' ddc: - '580' department: - _id: JiFr - _id: GradSch doi: 10.1126/science.adu8197 external_id: pmid: - '41990180' file: - access_level: open_access checksum: eb5b29247832ecdc53c8146da0509bbe content_type: application/pdf creator: dernst date_created: 2026-05-07T05:54:43Z date_updated: 2026-05-07T05:54:43Z file_id: '21832' file_name: 2026_Science_Kulich_accepted.pdf file_size: 6150733 relation: main_file success: 1 file_date_updated: 2026-05-07T05:54:43Z has_accepted_license: '1' intvolume: ' 392' issue: '6795' language: - iso: eng month: '04' oa: 1 oa_version: Accepted Version page: 296-300 pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Science publication_identifier: eissn: - 1095-9203 issn: - 0036-8075 publication_status: published publisher: AAAS quality_controlled: '1' scopus_import: '1' status: public title: Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 392 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21914' abstract: - lang: eng text: 'Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology.' acknowledged_ssus: - _id: MassSpec - _id: LifeSc acknowledgement: " We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-­HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-­HC2022/02 and Max-Planck-Society to A.S. " article_number: aea7828 article_processing_charge: Yes article_type: original author: - first_name: Mingyue full_name: Li, Mingyue id: 01f96916-0235-11eb-9379-a323192643b7 last_name: Li - first_name: Monika full_name: Chodasiewicz, Monika last_name: Chodasiewicz - first_name: Malavika full_name: Muraleedharan, Malavika last_name: Muraleedharan - first_name: Israel M. full_name: Lopez, Israel M. last_name: Lopez - first_name: Michal full_name: Gorka, Michal last_name: Gorka - first_name: Olga full_name: Kerber, Olga last_name: Kerber - first_name: Saqer S. full_name: Alotaibi, Saqer S. last_name: Alotaibi - first_name: Andrew D.L. full_name: Nelson, Andrew D.L. last_name: Nelson - first_name: Rene full_name: Lenobel, Rene last_name: Lenobel - first_name: Jaroslava full_name: Friedecká, Jaroslava last_name: Friedecká - first_name: Aleksandra full_name: Skirycz, Aleksandra last_name: Skirycz - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 2026;12(19). doi:10.1126/sciadv.aea7828 apa: Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.aea7828 chicago: Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” Science Advances. AAAS, 2026. https://doi.org/10.1126/sciadv.aea7828. ieee: M. Li et al., “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” Science Advances, vol. 12, no. 19. AAAS, 2026. ista: Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828. mla: Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” Science Advances, vol. 12, no. 19, aea7828, AAAS, 2026, doi:10.1126/sciadv.aea7828. short: M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026). corr_author: '1' date_created: 2026-05-24T22:01:31Z date_published: 2026-05-08T00:00:00Z date_updated: 2026-06-02T14:36:41Z day: '08' ddc: - '580' department: - _id: JiFr doi: 10.1126/sciadv.aea7828 external_id: pmid: - '42102187' file: - access_level: open_access checksum: 75b8ef2db078652c750e34e9cd98a808 content_type: application/pdf creator: dernst date_created: 2026-06-02T14:33:55Z date_updated: 2026-06-02T14:33:55Z file_id: '21941' file_name: 2026_ScienceAdv_Li2.pdf file_size: 2014452 relation: main_file success: 1 file_date_updated: 2026-06-02T14:33:55Z has_accepted_license: '1' intvolume: ' 12' issue: '19' language: - iso: eng month: '05' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Science Advances publication_identifier: eissn: - 2375-2548 publication_status: published publisher: AAAS quality_controlled: '1' scopus_import: '1' status: public title: Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 12 year: '2026' ... --- OA_type: closed access _id: '22293' abstract: - lang: eng text: In order to cope with arid terrestrial environments, angiosperms have evolved a unique fertilization way—siphonogamy. The success of siphonogamy requires several prerequisites, including the normal development of the female gametophyte and male gametophyte (pollen). Appropriate pollination methods ensure the successful encounter between pollen and the stigma. After pollen lands on the stigma, pollen/pollen tube interacts with different tissues and cells of the pistil, completing a series of male-female communications. The smooth progress of these interactions ensures that the pollen tube can enter the female gametophyte, burst, and release sperm cells to complete the double fertilization. In this chapter, we provide an overview of pollination and the interactions between male and female, comprehensively summarizing the factors involved in these processes. article_processing_charge: No author: - first_name: Sheng full_name: Zhong, Sheng last_name: Zhong - first_name: Zijun full_name: Lan, Zijun last_name: Lan - first_name: Zengxiang full_name: Ge, Zengxiang id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8 last_name: Ge orcid: 0000-0001-9381-3577 - first_name: Li-Jia full_name: Qu, Li-Jia last_name: Qu citation: ama: 'Zhong S, Lan Z, Ge Z, Qu L-J. Pollination and Fertilization. In: Chang F, Wang Y, Ma H, eds. Regulation of Plant Development. Singapore: Springer Nature; 2026:537-615. doi:10.1007/978-981-95-7033-1_14' apa: 'Zhong, S., Lan, Z., Ge, Z., & Qu, L.-J. (2026). Pollination and Fertilization. In F. Chang, Y. Wang, & H. Ma (Eds.), Regulation of Plant Development (pp. 537–615). Singapore: Springer Nature. https://doi.org/10.1007/978-981-95-7033-1_14' chicago: 'Zhong, Sheng, Zijun Lan, Zengxiang Ge, and Li-Jia Qu. “Pollination and Fertilization.” In Regulation of Plant Development, edited by Fang Chang, Yingxiang Wang, and Hong Ma, 537–615. Singapore: Springer Nature, 2026. https://doi.org/10.1007/978-981-95-7033-1_14.' ieee: 'S. Zhong, Z. Lan, Z. Ge, and L.-J. Qu, “Pollination and Fertilization,” in Regulation of Plant Development, F. Chang, Y. Wang, and H. Ma, Eds. Singapore: Springer Nature, 2026, pp. 537–615.' ista: 'Zhong S, Lan Z, Ge Z, Qu L-J. 2026.Pollination and Fertilization. In: Regulation of Plant Development. , 537–615.' mla: Zhong, Sheng, et al. “Pollination and Fertilization.” Regulation of Plant Development, edited by Fang Chang et al., Springer Nature, 2026, pp. 537–615, doi:10.1007/978-981-95-7033-1_14. short: S. Zhong, Z. Lan, Z. Ge, L.-J. Qu, in:, F. Chang, Y. Wang, H. Ma (Eds.), Regulation of Plant Development, Springer Nature, Singapore, 2026, pp. 537–615. das_tickbox: '1' date_created: 2026-07-13T09:46:18Z date_published: 2026-04-24T00:00:00Z date_updated: 2026-07-13T12:25:19Z day: '24' department: - _id: JiFr doi: 10.1007/978-981-95-7033-1_14 editor: - first_name: 'Fang ' full_name: 'Chang, Fang ' last_name: Chang - first_name: Yingxiang full_name: Wang, Yingxiang last_name: Wang - first_name: Hong full_name: Ma, Hong last_name: Ma language: - iso: eng month: '04' oa_version: None page: 537-615 place: Singapore publication: Regulation of Plant Development publication_identifier: eisbn: - '9789819570331' isbn: - '9789819570324' publication_status: published publisher: Springer Nature quality_controlled: '1' scopus_import: '1' status: public title: Pollination and Fertilization type: book_chapter user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '22301' abstract: - lang: eng text: Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage. acknowledged_ssus: - _id: Bio - _id: NanoFab acknowledgement: Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria. article_number: jipb.70309 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Adrijana full_name: Smoljan, Adrijana id: cced8a85-223e-11ed-af04-b0596c55053b last_name: Smoljan - first_name: Sarah full_name: Koutnik‐Abele, Sarah last_name: Koutnik‐Abele - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Petr full_name: Klíma, Petr last_name: Klíma - first_name: Anita full_name: Bírošíková, Anita last_name: Bírošíková - first_name: Yuzhou full_name: Zhang, Yuzhou id: 3B6137F2-F248-11E8-B48F-1D18A9856A87 last_name: Zhang orcid: 0000-0003-2627-6956 - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Maximilian full_name: Schuster, Maximilian id: 37e65def-d415-11eb-ae59-a7b67be103db last_name: Schuster - first_name: Katarina full_name: Kurtović, Katarina last_name: Kurtović - first_name: Ulrich Z. full_name: Hammes, Ulrich Z. last_name: Hammes - first_name: Jan full_name: Petrášek, Jan last_name: Petrášek - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026. doi:10.1111/jipb.70309 apa: Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309 chicago: Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology. Wiley, 2026. https://doi.org/10.1111/jipb.70309. ieee: A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte algae,” Journal of Integrative Plant Biology. Wiley, 2026. ista: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309. mla: Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026, doi:10.1111/jipb.70309. short: A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026). corr_author: '1' das_tickbox: '0' date_created: 2026-07-13T10:44:55Z date_published: 2026-06-10T00:00:00Z date_updated: 2026-07-13T14:26:31Z day: '10' ddc: - '580' department: - _id: JiFr - _id: GradSch - _id: NanoFab - _id: Bio doi: 10.1111/jipb.70309 external_id: pmid: - '42271607' has_accepted_license: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1111/jipb.70309 month: '06' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Journal of Integrative Plant Biology publication_identifier: eissn: - 1744-7909 issn: - 1672-9072 publication_status: epub_ahead publisher: Wiley quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: yes title: Auxin response and PIN‐mediated transport in chlorophyte algae tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_type: closed access _id: '22366' abstract: - lang: eng text: Gravitropism is a fundamental adaptive response in plants that enables directional growth to optimize resource acquisition. In this study, we employed forward genetic screening to identify Arabidopsis mutants with defective hypocotyl gravitropism and isolated the short and agravitropic hypocotyl in dark1 (sad1) mutant, which carries a point mutation (G110E) in the SAC1 gene encoding a phosphoinositide phosphatase. Deficiency of SAC1 disrupted gravity-induced polar localization of PIN3 in endodermal cells, impairing auxin redistribution and leading to hypocotyl gravitropism defects. Subcellular localization analysis revealed that SAC1 is partially localized to the PVC/tonoplast and participates in late endosomal trafficking. The sac1 mutation leads to abnormal vacuolar morphology, which is associated with defects in amyloplast sedimentation during the gravitropic response in Arabidopsis shoots. We further revealed that SAC1 interacts with GRV2, a key regulator of the late endocytic pathway, and that both proteins cooperatively regulate shoot gravitropism. In summary, this study identified SAC1 as a regulator of shoot gravitropism, revealing its important role in modulating vacuolar homeostasis, amyloplast sedimentation, PIN3 trafficking, and auxin distribution. These findings provide insights into the molecular mechanisms linking membrane transport to environmental adaptation in plants. acknowledgement: We acknowledge Prof. Dolf Weijers (Wageningen University), Prof.Karin Schumacher (Heidelberg University), Prof. Yohann Boutt ´e(Universit ´e de Bordeaux), and Prof. Jinbo Shen (Zhejiang A&FUniversity) for providing published plasmids and Arabidopsislines. We thank Dr. Gergely Moln ´ar (ISTA) for help with NGS dataanalysis, and Prof. Jianru Zuo (IGDB, CAS), Prof. Chengbin Xiang(USTC), and Prof. Zhong Zhao (USTC) for critical comments onthe manuscript. We thank the staff members of the Mass Spec-trometry System at the National Facility for Protein Science inShanghai (NFPS), Zhangjiang Lab, China for providing technicalsupport and assistance in data collection and analysis. This workwas supported by grants from the National Natural Science Foun-dation of China (32570366, and 32321001 to ST), the Natural Sci-ence Foundation of Anhui Province (2508085QC070 to MK), theFundamental Research Funds for the Central Universities(WK9100250095 to MK, and WK9100000021 to ST), the ForestryBureau of Anhui Province (AHLYJBGS-2024-01 to ST), the Centerfor Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine, University of Science andTechnology of China (QYPY20220012 to ST), the USTC ResearchFunds of the Double First-Class Initiative (YD9100002016 to ST),and start-up funding from the University of Science and Technol-ogy of China and the Chinese Academy of Sciences(GG9100007007, KY9100000026, KY9100000051, XKTS-202591014,XKTS-2026910122, and KJ2070000079 to ST). article_number: e71042 article_processing_charge: No article_type: original author: - first_name: Lianghanxiao full_name: Sun, Lianghanxiao last_name: Sun - first_name: Wenxin full_name: Jia, Wenxin last_name: Jia - first_name: Yanbo full_name: Mao, Yanbo last_name: Mao - first_name: Xin full_name: Li, Xin last_name: Li - first_name: Mengjuan full_name: Kong, Mengjuan last_name: Kong - first_name: Ji full_name: She, Ji last_name: She - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Shutang full_name: Tan, Shutang id: 2DE75584-F248-11E8-B48F-1D18A9856A87 last_name: Tan orcid: 0000-0002-0471-8285 citation: ama: Sun L, Jia W, Mao Y, et al. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 2026;127(1). doi:10.1111/tpj.71042 apa: Sun, L., Jia, W., Mao, Y., Li, X., Kong, M., She, J., … Tan, S. (2026). Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. Wiley. https://doi.org/10.1111/tpj.71042 chicago: Sun, Lianghanxiao, Wenxin Jia, Yanbo Mao, Xin Li, Mengjuan Kong, Ji She, Jiří Friml, and Shutang Tan. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal. Wiley, 2026. https://doi.org/10.1111/tpj.71042. ieee: L. Sun et al., “Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis,” The Plant Journal, vol. 127, no. 1. Wiley, 2026. ista: Sun L, Jia W, Mao Y, Li X, Kong M, She J, Friml J, Tan S. 2026. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 127(1), e71042. mla: Sun, Lianghanxiao, et al. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal, vol. 127, no. 1, e71042, Wiley, 2026, doi:10.1111/tpj.71042. short: L. Sun, W. Jia, Y. Mao, X. Li, M. Kong, J. She, J. Friml, S. Tan, The Plant Journal 127 (2026). das_tickbox: '1' dataavailabilitystatement: Biological materials (seeds, plasmids) are available upon request from ST (sttan@ustc.edu.cn). The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. date_created: 2026-07-19T22:01:47Z date_published: 2026-07-01T00:00:00Z date_updated: 2026-07-20T13:53:44Z day: '01' department: - _id: JiFr doi: 10.1111/tpj.71042 external_id: pmid: - '42438075' intvolume: ' 127' issue: '1' keyword: - auxin - SAC1 - GRV2 - PIN3 - vacuole - gravitropism - Arabidopsis language: - iso: eng month: '07' oa_version: None pmid: 1 publication: The Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 127 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20725' abstract: - lang: eng text: The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article. acknowledgement: I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input. article_processing_charge: Yes (via OA deal) article_type: review author: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: 'Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 2026;31(2):136-138. doi:10.1016/j.tplants.2025.10.018' apa: 'Friml, J. (2026). Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2025.10.018' chicago: 'Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science. Elsevier, 2026. https://doi.org/10.1016/j.tplants.2025.10.018.' ieee: 'J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” Trends in Plant Science, vol. 31, no. 2. Elsevier, pp. 136–138, 2026.' ista: 'Friml J. 2026. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 31(2), 136–138.' mla: 'Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science, vol. 31, no. 2, Elsevier, 2026, pp. 136–38, doi:10.1016/j.tplants.2025.10.018.' short: J. Friml, Trends in Plant Science 31 (2026) 136–138. corr_author: '1' das_tickbox: '0' date_created: 2025-12-02T16:29:22Z date_published: 2026-02-01T00:00:00Z date_updated: 2026-07-27T08:27:07Z day: '01' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.tplants.2025.10.018 external_id: pmid: - '41249070' file: - access_level: open_access checksum: e60e903fb4b3e917dba763976ec652cd content_type: application/pdf creator: dernst date_created: 2026-07-27T08:26:40Z date_updated: 2026-07-27T08:26:40Z file_id: '22413' file_name: 2026_TrendsPlantScience_Friml.pdf file_size: 432792 relation: main_file success: 1 file_date_updated: 2026-07-27T08:26:40Z has_accepted_license: '1' intvolume: ' 31' issue: '2' language: - iso: eng month: '02' oa: 1 oa_version: Published Version page: 136-138 pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Trends in Plant Science publication_identifier: eissn: - 1878-4372 issn: - 1360-1385 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: 'Role of cAMP in TIR1/AFB auxin signaling: Open issues' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 31 year: '2026' ... --- OA_type: closed access _id: '20636' abstract: - lang: eng text: The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis. acknowledgement: We thank Lukáš Fiedler‬ for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X. article_processing_charge: No article_type: original author: - first_name: R full_name: Huang, R last_name: Huang - first_name: J full_name: Wang, J last_name: Wang - first_name: M full_name: Chang, M last_name: Chang - first_name: W full_name: Tang, W last_name: Tang - first_name: Y full_name: Yu, Y last_name: Yu - first_name: Y full_name: Zhang, Y last_name: Zhang - first_name: Y full_name: Peng, Y last_name: Peng - first_name: Y full_name: Wang, Y last_name: Wang - first_name: Y full_name: Guo, Y last_name: Guo - first_name: T full_name: Lu, T last_name: Lu - first_name: Y full_name: Cao, Y last_name: Cao - first_name: Y full_name: Zhou, Y last_name: Zhou - first_name: Q full_name: Zhang, Q last_name: Zhang - first_name: Y full_name: Huang, Y last_name: Huang - first_name: A full_name: Wu, A last_name: Wu - first_name: L full_name: Ren, L last_name: Ren - first_name: Michelle C full_name: Gallei, Michelle C id: 35A03822-F248-11E8-B48F-1D18A9856A87 last_name: Gallei orcid: 0000-0003-1286-7368 - first_name: J full_name: Dong, J last_name: Dong - first_name: H full_name: Chen, H last_name: Chen - first_name: J full_name: He, J last_name: He - first_name: M full_name: Wen, M last_name: Wen - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: L full_name: Sun, L last_name: Sun - first_name: Y full_name: Xiong, Y last_name: Xiong - first_name: Z full_name: Yang, Z last_name: Yang - first_name: T full_name: Xu, T last_name: Xu citation: ama: Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 2026;61(1):73-84. doi:10.1016/j.devcel.2025.09.009 apa: Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2026). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.09.009 chicago: Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell. Elsevier, 2026. https://doi.org/10.1016/j.devcel.2025.09.009. ieee: R. Huang et al., “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” Developmental Cell, vol. 61, no. 1. Elsevier, pp. 73–84, 2026. ista: Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2026. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 61(1), 73–84. mla: Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell, vol. 61, no. 1, Elsevier, 2026, pp. 73–84, doi:10.1016/j.devcel.2025.09.009. short: R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell 61 (2026) 73–84. das_tickbox: '1' dataavailabilitystatement: "Plasmids and genetic materials generated in this study will be made available upon request from the lead contact.The mass spectrometry data reported in this work have been deposited at the iProX database. Original western blot gel images have been deposited to Mendeley Data. The accession code and the DOI are listed in the key resources table.\r\nThis paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request." date_created: 2025-11-12T10:03:39Z date_published: 2026-01-14T00:00:00Z date_updated: 2026-07-27T08:02:04Z day: '14' department: - _id: JiFr doi: 10.1016/j.devcel.2025.09.009 external_id: pmid: - '41043435' intvolume: ' 61' issue: '1' language: - iso: eng month: '01' oa_version: None page: 73-84 pmid: 1 publication: Developmental Cell publication_identifier: eissn: - 1878-1551 issn: - 1534-5807 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 61 year: '2026' ... --- OA_type: closed access _id: '20818' abstract: - lang: eng text: "This study demonstrates that Marchantia non-canonical PINs are predominantly localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric distribution.\r\nA newly identified miniW domain within the MpPINW hydrophilic loop governs subcellular trafficking and asymmetric PM localization of non-canonical PINs in Marchantia." acknowledgement: The authors sincerely thank Dr. Shutang Tan for experimental support and Dr. Barbara Kloeckener Gruissem for critical reading and constructive advice on the manuscript. This study was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.), by the Ministry of Science and Technology (grant 112-2636-B-005-001- to K.-J.L.), and by the Ministry of Education (grant MOE-109-YSFAG-0006-001-P1 to K.-J.L.). article_processing_charge: No article_type: comment author: - first_name: Han full_name: Tang, Han id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E last_name: Tang orcid: 0000-0001-6152-6637 - first_name: Adrijana full_name: Smoljan, Adrijana id: cced8a85-223e-11ed-af04-b0596c55053b last_name: Smoljan - first_name: Minxia full_name: Zou, Minxia id: 5c243f41-03f3-11ec-841c-96faf48a7ef9 last_name: Zou - first_name: Yuzhou full_name: Zhang, Yuzhou id: 3B6137F2-F248-11E8-B48F-1D18A9856A87 last_name: Zhang orcid: 0000-0003-2627-6956 - first_name: Kuan Ju full_name: Lu, Kuan Ju last_name: Lu - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. 2026;49(3):1505-1508. doi:10.1111/pce.70295 apa: Tang, H., Smoljan, A., Zou, M., Zhang, Y., Lu, K. J., & Friml, J. (2026). The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. Wiley. https://doi.org/10.1111/pce.70295 chicago: Tang, Han, Adrijana Smoljan, Minxia Zou, Yuzhou Zhang, Kuan Ju Lu, and Jiří Friml. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment. Wiley, 2026. https://doi.org/10.1111/pce.70295. ieee: H. Tang, A. Smoljan, M. Zou, Y. Zhang, K. J. Lu, and J. Friml, “The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha,” Plant Cell and Environment, vol. 49, no. 3. Wiley, pp. 1505–1508, 2026. ista: Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. 2026. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. 49(3), 1505–1508. mla: Tang, Han, et al. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment, vol. 49, no. 3, Wiley, 2026, pp. 1505–08, doi:10.1111/pce.70295. short: H. Tang, A. Smoljan, M. Zou, Y. Zhang, K.J. Lu, J. Friml, Plant Cell and Environment 49 (2026) 1505–1508. das_tickbox: '1' dataavailabilitystatement: The data that support the findings of this study are available from the corresponding author upon reasonable request. date_created: 2025-12-14T23:02:05Z date_published: 2026-03-01T00:00:00Z date_updated: 2026-07-27T10:27:47Z day: '01' department: - _id: JiFr doi: 10.1111/pce.70295 ec_funded: 1 external_id: pmid: - '41340422' intvolume: ' 49' issue: '3' language: - iso: eng month: '03' oa_version: None page: 1505-1508 pmid: 1 project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants publication: Plant Cell and Environment publication_identifier: eissn: - 1365-3040 issn: - 0140-7791 publication_status: published publisher: Wiley quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 49 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '21483' abstract: - lang: eng text: 'Embryogenesis in the model plant Arabidopsis thaliana provides a framework for understanding how cell polarity and patterning coordinate with hormonal signalling to establish the plant body plan. Following fertilisation, the zygote divides asymmetrically to generate apical and basal lineages, establishing the apical–basal axis that defines future shoot and root poles. Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical–basal axis establishment and organ initiation. The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors. Advances in microscopy have transformed the study of Arabidopsis embryogenesis: fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation, and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development, while laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity. Together, these methodological breakthroughs position Arabidopsis embryos as a prime model for dissecting the chemical and biophysical cues that shape plant development.' acknowledgement: The authors would like to acknowledge the many colleagues whose valuable contributions to the field could not be included in this review due to space limitations and reference constraints. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: David full_name: Babic, David id: db566d23-f6e0-11ea-865d-e6f270e968e7 last_name: Babic - first_name: Milan full_name: Zupunski, Milan id: f6a21fce-573e-11f0-a150-a8d96aee2539 last_name: Zupunski - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 2026;250(3):1483-1491. doi:10.1111/nph.71072 apa: Babic, D., Zupunski, M., & Friml, J. (2026). Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. Wiley. https://doi.org/10.1111/nph.71072 chicago: Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist. Wiley, 2026. https://doi.org/10.1111/nph.71072. ieee: D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study Arabidopsis embryogenesis,” New Phytologist, vol. 250, no. 3. Wiley, pp. 1483–1491, 2026. ista: Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 250(3), 1483–1491. mla: Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist, vol. 250, no. 3, Wiley, 2026, pp. 1483–91, doi:10.1111/nph.71072. short: D. Babic, M. Zupunski, J. Friml, New Phytologist 250 (2026) 1483–1491. corr_author: '1' das_tickbox: '0' date_created: 2026-03-23T14:59:06Z date_published: 2026-05-01T00:00:00Z date_updated: 2026-07-27T11:47:24Z day: '01' ddc: - '580' department: - _id: JiFr - _id: GradSch doi: 10.1111/nph.71072 external_id: pmid: - '41808651' file: - access_level: open_access checksum: 67513dde983631bed9613fdaadbbee06 content_type: application/pdf creator: dernst date_created: 2026-07-27T11:46:25Z date_updated: 2026-07-27T11:46:25Z file_id: '22424' file_name: 2026_NewPhytologist_Babic.pdf file_size: 1708392 relation: main_file success: 1 file_date_updated: 2026-07-27T11:46:25Z has_accepted_license: '1' intvolume: ' 250' issue: '3' language: - iso: eng month: '05' oa: 1 oa_version: Published Version page: 1483-1491 pmid: 1 publication: New Phytologist publication_identifier: eissn: - 1469-8137 issn: - 0028-646X publication_status: published publisher: Wiley quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: Imaging and genetic toolbox to study Arabidopsis embryogenesis tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 250 year: '2026' ... --- OA_place: publisher OA_type: hybrid _id: '22647' abstract: - lang: eng text: "Within the plant endomembrane system, the vesicle coat protein clathrin localizes to the plasma membrane (PM) and the trans-Golgi Network/early endosome (TGN/EE). While the role of clathrin in endocytosis at the PM is well established, its function at TGN/EE, presumably in late secretion (trafficking from the TGN/EE to the cell surface) or en route to the vacuole, is debated. Similarly debated are potential homeostatic mechanisms balancing the trafficking routes, especially endocytosis and late secretion.\r\nWe address these questions in Arabidopsis thaliana using conditional silencing of CLATHRIN HEAVY CHAIN (CHC), conditional overexpression of the clathrin uncoating factor AUXILIN-LIKE1, and secretory mutants.\r\nCHC silencing interferes with trafficking of cargoes destined for the apoplast and the PM, supporting a function of clathrin in late secretion. The secretory cargoes become abnormally rerouted from the TGN/EE to the vacuole. Unlike CHC silencing, overexpression of AUXILIN-LIKE1 selectively inhibits clathrin-mediated endocytosis while secretion continues normally at early points of induction. Conversely, secretory mutants exhibit a reduced PM recruitment of clathrin, and variably, of the TPLATE endocytic component.\r\nTogether, our data show a role of clathrin in secretion and suggest secretion as a fundamental trafficking process to which endocytosis is adjusted by a weak homeostatic mechanism." acknowledgement: 'The authors wish to acknowledge Dr. Paweł Baster for cloning PIN1-GFP-2/pDONR221, Ms. Aline Monzer and Dr. Mingyue Li for help with CHC protein level evaluation, Dr. Michał Rychłowski for help with confocal microscopy, Prof. Ari Pekka Mähönen for sharing the p1R4-pUBQ10:XVE plasmid, and Prof. Ying Gu for sharing seeds of the sec5 mutant. M.A. would like to thank Dr. Xixi Zhang and Prof. Sebastian Bednarek for inspiring discussions. This work was supported by the Taif University Researchers Supporting Project, TURSP-HC2022/02 to JF and SA and Austrian Science Fund (FWF): I 3630-B25 to JF. Open Access funding provided by Institute of Science and Technology Austria.' article_number: nph.71454 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Maciek full_name: Adamowski, Maciek id: 45F536D2-F248-11E8-B48F-1D18A9856A87 last_name: Adamowski orcid: 0000-0001-6463-5257 - first_name: Adam full_name: Gackowski, Adam last_name: Gackowski - first_name: Ivana full_name: Matijevic, Ivana id: 83c17ce3-15b2-11ec-abd3-f486545870bd last_name: Matijevic - first_name: Saqer S. full_name: Alotaibi, Saqer S. last_name: Alotaibi - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. The role of clathrin in post‐Golgi secretion in plant cells. New Phytologist. 2026. doi:10.1111/nph.71454 apa: Adamowski, M., Gackowski, A., Matijevic, I., Alotaibi, S. S., & Friml, J. (2026). The role of clathrin in post‐Golgi secretion in plant cells. New Phytologist. Wiley. https://doi.org/10.1111/nph.71454 chicago: Adamowski, Maciek, Adam Gackowski, Ivana Matijevic, Saqer S. Alotaibi, and Jiří Friml. “The Role of Clathrin in Post‐Golgi Secretion in Plant Cells.” New Phytologist. Wiley, 2026. https://doi.org/10.1111/nph.71454. ieee: M. Adamowski, A. Gackowski, I. Matijevic, S. S. Alotaibi, and J. Friml, “The role of clathrin in post‐Golgi secretion in plant cells,” New Phytologist. Wiley, 2026. ista: Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. 2026. The role of clathrin in post‐Golgi secretion in plant cells. New Phytologist., nph. 71454. mla: Adamowski, Maciek, et al. “The Role of Clathrin in Post‐Golgi Secretion in Plant Cells.” New Phytologist, nph. 71454, Wiley, 2026, doi:10.1111/nph.71454. short: M. Adamowski, A. Gackowski, I. Matijevic, S.S. Alotaibi, J. Friml, New Phytologist (2026). corr_author: '1' das_tickbox: '1' dataavailabilitystatement: 'Original data associated with this study have been deposited in Dataset S1. The accession nos. of A. thaliana genes used in this study are as follows: CHC1 (AT3G11130), CHC2 (AT3G08530), CLC2 (AT2G40060), TPLATE (AT3G01780), AP2A1 (AT5G22770), DRP1C (AT1G14830), GNOM-LIKE1 (AT5G39500), BEN3/BIG2 (AT3G60860), TMK4 (AT3G23750), PIN1 (AT1G73590), AUXILIN-LIKE1 (AT4G12780), AP1M2 (AT1G60780), ECHIDNA (AT1G09330), SEC5A (AT1G76850), SEC5B (AT1G21170), TUB2 (AT5G62690), and PP2AA3 (AT1G13320).' date_created: 2026-08-04T06:48:41Z date_published: 2026-07-20T00:00:00Z date_updated: 2026-08-04T07:58:27Z day: '20' department: - _id: JiFr - _id: MaLo - _id: GradSch doi: 10.1111/nph.71454 external_id: pmid: - '42477503' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1111/nph.71454 month: '07' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 26538374-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: I03630 name: Molecular mechanisms of endocytic cargo recognition in plants publication: New Phytologist publication_identifier: eissn: - 1469-8137 issn: - 0028-646X publication_status: epub_ahead publisher: Wiley quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: The role of clathrin in post‐Golgi secretion in plant cells type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_type: closed access _id: '22315' abstract: - lang: eng text: Plant tropisms enable roots to navigate complex soils by responding to directional environmental cues. Biological decay, although central to nutrient cycling, also creates microbially active and potentially hostile niches. In this work, we identified “saprotropism,” a previously unrecognized growth response that enables roots to actively bend away from decaying plant-derived matter. Fungal-driven microbial decomposition released organic acids and formed stable pH gradients in surrounding soil, allowing roots to pinpoint decay without direct contact. Root epidermal cells sensed this acidic gradient through the root meristem growth factor peptide-receptor module, converting external pH asymmetry into asymmetric abscisic acid (ABA) distribution. ABA asymmetry drove microtubule reorganization, which was decoded into decay-avoidant root bending. Together, these findings establish microbial decay–derived chemical gradients as an instructive signal for root navigation and expand the framework of microbe-soil-plant communication. acknowledgement: 'We are grateful to H. Guo and L. Liu (Department of Biology, Southern University of Science and Technology) for providing the rgf1/2/3, rgi1/2/3/4, tpst-1, and pepr1/2 lines. We thank K.-h. Liu (College of Life Science, Northwest A&F University) for generously providing the ABA biosensor nlsABACUS2-400n. We also thank J. Li and J. Chang (School of Life Sciences, Lanzhou University) for providing the ahk2-5/cre1-2, ahp1/2/3, arr16/arr17, and pTCSn::GFP lines. Our thanks further extend to D. Qian, also from the School of Life Sciences at Lanzhou University, for sharing Arabidopsis line pTUB6::mCherry-TUB6. We are grateful to Y. Zhao (CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences) for providing nced3/5, snrk2.2/2.3/2.6, and pyl duodecuple mutants. We also acknowledge the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly N. Fan, for their invaluable technical assistance. We also thank Life Science Research Core Services (LSRCS), Northwest A&F University, for helping with characterization, including CLSM (X. Liu). Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China JYB2025XDXM706 (Y.Z.); Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program QCYRCXM-2022-237 (Y.Z.); Fundamental Research Funds for the Central Universities K20200168 (Y.Z.); National Natural Science Foundation of China 32570375 (Y.Z.); National Natural Science Foundation of China 32400699 (A.Z.); European Research Council (ERC, CYNIPS) 101142681 (J.F.); Austrian Science Fund (FWF): P 37051-B (J.F.).' article_number: eadw6568 article_processing_charge: No article_type: original author: - first_name: Zhulatai full_name: Bao, Zhulatai last_name: Bao - first_name: Huihui full_name: Wang, Huihui last_name: Wang - first_name: Ai full_name: Zhang, Ai last_name: Zhang - first_name: Ruxi full_name: Gao, Ruxi last_name: Gao - first_name: Wen full_name: Gu, Wen last_name: Gu - first_name: Ni full_name: Fan, Ni last_name: Fan - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Yuzhou full_name: Zhang, Yuzhou last_name: Zhang citation: ama: Bao Z, Wang H, Zhang A, et al. Roots navigate around decay regions by sensing local pH gradients. Science. 2026;393(6807). doi:10.1126/science.adw6568 apa: Bao, Z., Wang, H., Zhang, A., Gao, R., Gu, W., Fan, N., … Zhang, Y. (2026). Roots navigate around decay regions by sensing local pH gradients. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.adw6568 chicago: Bao, Zhulatai, Huihui Wang, Ai Zhang, Ruxi Gao, Wen Gu, Ni Fan, Jiří Friml, and Yuzhou Zhang. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” Science. American Association for the Advancement of Science, 2026. https://doi.org/10.1126/science.adw6568. ieee: Z. Bao et al., “Roots navigate around decay regions by sensing local pH gradients,” Science, vol. 393, no. 6807. American Association for the Advancement of Science, 2026. ista: Bao Z, Wang H, Zhang A, Gao R, Gu W, Fan N, Friml J, Zhang Y. 2026. Roots navigate around decay regions by sensing local pH gradients. Science. 393(6807), eadw6568. mla: Bao, Zhulatai, et al. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” Science, vol. 393, no. 6807, eadw6568, American Association for the Advancement of Science, 2026, doi:10.1126/science.adw6568. short: Z. Bao, H. Wang, A. Zhang, R. Gao, W. Gu, N. Fan, J. Friml, Y. Zhang, Science 393 (2026). das_tickbox: '1' dataavailabilitystatement: All data are available in the manuscript or the supplementary materials. The raw RNA-seq data have been deposited in the NCBI Gene Expression Omnibus (GEO) under accession number GSE315473. Microbiome sequencing data have been deposited in the Sequence Read Archive (SRA) under BioProject number PRJNA1397137. Materials are available upon request from the corresponding author. date_created: 2026-07-13T14:57:10Z date_published: 2026-07-09T00:00:00Z date_updated: 2026-08-04T09:22:49Z day: '09' department: - _id: JiFr doi: 10.1126/science.adw6568 external_id: pmid: - '42424472' intvolume: ' 393' issue: '6807' language: - iso: eng month: '07' oa_version: None pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Science publication_identifier: eissn: - 1095-9203 issn: - 0036-8075 publication_status: published publisher: American Association for the Advancement of Science quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/roots-steer-clear-of-plant-rot/ researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Roots navigate around decay regions by sensing local pH gradients type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 393 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '22712' abstract: - lang: eng text: Ginseng (Panax ginseng) derives its renowned therapeutic properties from ginsenoside metabolites. However, the long cultivation cycle and susceptibility to diseases hinder the advancement of the ginseng industry. Here, we demonstrate that the embryonic protoderm of ginseng can efficiently produce ginsenosides. Single-cell transcriptome and mass spectrometry imaging analyses reveal that ginsenosides accumulate in the protoderm of ginseng embryonic callus (EC) at levels comparable to those in forest ginseng. Epigenetic analyses indicate that elevated histone acetylation and enhanced chromatin accessibility at regeneration- and ginsenoside metabolism-related gene loci are associated with the ginsenoside-producing capacity of EC. Increasing histone acetylation levels or overexpressing the regeneration-related WUSCHEL-RELATED HOMEOBOX11 (WOX11) gene further enhances ginsenoside production in EC. Our findings suggest that the protoderm of EC could serve as an in situ biological compartment for high-efficiency ginsenoside producion, offering a complementary approach to traditional ginseng cultivation. acknowledgement: 'The authors are grateful to Professor Linfeng Li from the School of Life Science, Fudan University, for his assistance during the ginseng genome annotation. This work was supported by Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302-2401-08 to L.X. and J.L.), the National Natural Science Foundation of China (82373987 to J.L., 31701294 to L.X., 32225007 to L.X., and 32300285 to N.Z.), the Fundamental Research Funds for the Central public welfare research institutes (ZZ13-YQ-093, ZZXT202508 to J.L.), the CACMS Innovation Fund (CI2025G00-06 to J.L.), the Principle Investigator Program (HBMUPI202104 to Y.Z.), the Key R&D Program of Shandong Province, China (2024LZGC025 to L.X.), the National Key R&D Program of China (2024YFF1000700/2023YFE0101100 to L.X.), Strategic Priority Research Program of Chinese Academy of Sciences (XDB0630000 to L.X.), and China Postdoctoral Science Foundation (2023M733490 to N.Z.).' article_number: '7994' article_processing_charge: Yes article_type: original author: - first_name: Juan full_name: Liu, Juan last_name: Liu - first_name: Ning full_name: Zhai, Ning last_name: Zhai - first_name: Shiyi full_name: Zhang, Shiyi last_name: Zhang - first_name: Yosuke full_name: Tamada, Yosuke last_name: Tamada - first_name: Tonghui full_name: Li, Tonghui last_name: Li - first_name: Linfan full_name: Zhang, Linfan last_name: Zhang - first_name: Tong full_name: Chen, Tong last_name: Chen - first_name: Chenglin full_name: Wang, Chenglin last_name: Wang - first_name: Jian full_name: Yang, Jian last_name: Yang - first_name: Jiaqi full_name: Gao, Jiaqi last_name: Gao - first_name: Xiang full_name: Li, Xiang id: 4B7E523C-F248-11E8-B48F-1D18A9856A87 last_name: Li - first_name: Junhui full_name: Zhou, Junhui last_name: Zhou - first_name: Yonghong full_name: Zhang, Yonghong last_name: Zhang - first_name: Yu full_name: Liu, Yu id: 2A70014E-F248-11E8-B48F-1D18A9856A87 last_name: Liu orcid: 0000-0001-7313-6740 - first_name: Yuan full_name: Wang, Yuan last_name: Wang - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Chen full_name: Li, Chen last_name: Li - first_name: Lin full_name: Xu, Lin last_name: Xu - first_name: Luqi full_name: Huang, Luqi last_name: Huang citation: ama: Liu J, Zhai N, Zhang S, et al. Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. Nature Communications. 2026;17. doi:10.1038/s41467-026-74881-5 apa: Liu, J., Zhai, N., Zhang, S., Tamada, Y., Li, T., Zhang, L., … Huang, L. (2026). Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-74881-5 chicago: Liu, Juan, Ning Zhai, Shiyi Zhang, Yosuke Tamada, Tonghui Li, Linfan Zhang, Tong Chen, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm as a Native Compartment for High-Efficiency Ginsenoside Production.” Nature Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-74881-5. ieee: J. Liu et al., “Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production,” Nature Communications, vol. 17. Springer Nature, 2026. ista: Liu J, Zhai N, Zhang S, Tamada Y, Li T, Zhang L, Chen T, Wang C, Yang J, Gao J, Li X, Zhou J, Zhang Y, Liu Y, Wang Y, Friml J, Benková E, Li C, Xu L, Huang L. 2026. Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. Nature Communications. 17, 7994. mla: Liu, Juan, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm as a Native Compartment for High-Efficiency Ginsenoside Production.” Nature Communications, vol. 17, 7994, Springer Nature, 2026, doi:10.1038/s41467-026-74881-5. short: J. Liu, N. Zhai, S. Zhang, Y. Tamada, T. Li, L. Zhang, T. Chen, C. Wang, J. Yang, J. Gao, X. Li, J. Zhou, Y. Zhang, Y. Liu, Y. Wang, J. Friml, E. Benková, C. Li, L. Xu, L. Huang, Nature Communications 17 (2026). das_tickbox: '1' dataavailabilitystatement: The RNA-seq data generated in this study have been deposited in the GSA database under accession code CRA008967. The scRNA-seq data generated in this study have been deposited in the GSA database under accession code CRA026200. The ATAC-seq data generated in this study have been deposited in the GSA database under accession code CRA008969. The ChIP-seq data generated in this study have been deposited in the GSA database under accession code CRA008968. Single-cell RNA-seq data and scripts are publicly available on Zenodo (https://zenodo.org/records/20392012). Primers are in Supplemental Table 5. Source data are provided with this paper. date_created: 2026-08-16T22:01:42Z date_published: 2026-08-07T00:00:00Z date_updated: 2026-08-20T06:45:46Z day: '07' ddc: - '580' department: - _id: JiFr - _id: EvBe doi: 10.1038/s41467-026-74881-5 external_id: pmid: - '42350386' file: - access_level: open_access checksum: 0b4ff29f0d0168b11be32a5f22ded9e4 content_type: application/pdf creator: dernst date_created: 2026-08-20T06:44:33Z date_updated: 2026-08-20T06:44:33Z file_id: '22742' file_name: 2026_NatureComm_Liu.pdf file_size: 2429551 relation: main_file success: 1 file_date_updated: 2026-08-20T06:44:33Z has_accepted_license: '1' intvolume: ' 17' language: - iso: eng month: '08' oa: 1 oa_version: Published Version pmid: 1 publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 17 year: '2026' ... --- OA_place: repository _id: '20982' abstract: - lang: eng text: Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants. acknowledgement: "This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n" article_processing_charge: No author: - first_name: Ivan full_name: Kulich, Ivan id: 57a1567c-8314-11eb-9063-c9ddc3451a54 last_name: Kulich - first_name: Denisa full_name: Oulehlová, Denisa last_name: Oulehlová - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Minxia full_name: Zou, Minxia id: 5c243f41-03f3-11ec-841c-96faf48a7ef9 last_name: Zou - first_name: Edita full_name: Lileikyte, Edita last_name: Lileikyte - first_name: Alexey full_name: Bondar, Alexey last_name: Bondar - first_name: Katarína full_name: Kulichová, Katarína last_name: Kulichová - first_name: Martin full_name: Janda, Martin last_name: Janda - first_name: Oksana full_name: Iakovenko, Oksana last_name: Iakovenko - first_name: Michaela full_name: Neubergerová, Michaela last_name: Neubergerová - first_name: Tanja full_name: Studtrucker, Tanja last_name: Studtrucker - first_name: Roman full_name: Pleskot, Roman last_name: Pleskot - first_name: Petra full_name: Dietrich, Petra last_name: Dietrich - first_name: Matyas full_name: Fendrych, Matyas id: 43905548-F248-11E8-B48F-1D18A9856A87 last_name: Fendrych orcid: 0000-0002-9767-8699 - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. doi:10.1101/2025.01.06.631460 apa: Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. https://doi.org/10.1101/2025.01.06.631460 chicago: Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, n.d. https://doi.org/10.1101/2025.01.06.631460. ieee: I. Kulich et al., “Armadillo repeat only proteins are required for the function of plant CNGC channels,” bioRxiv. . ista: Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, 10.1101/2025.01.06.631460. mla: Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, doi:10.1101/2025.01.06.631460. short: I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.). corr_author: '1' date_created: 2026-01-13T14:07:58Z date_published: 2025-05-16T00:00:00Z date_updated: 2026-04-07T11:41:43Z day: '16' department: - _id: JiFr doi: 10.1101/2025.01.06.631460 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/2025.01.06.631460 month: '05' oa: 1 oa_version: Preprint publication: bioRxiv publication_status: draft related_material: record: - id: '20964' relation: dissertation_contains status: public status: public title: Armadillo repeat only proteins are required for the function of plant CNGC channels tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: preprint user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher OA_type: hybrid _id: '21136' abstract: - lang: eng text: The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development. acknowledgement: We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript. article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Aline full_name: Monzer, Aline id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425 last_name: Monzer - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 2025;1(1):2. doi:10.1038/s44383-025-00002-8 apa: Monzer, A., & Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. Npj Science of Plants. Springer Nature. https://doi.org/10.1038/s44383-025-00002-8 chicago: Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants. Springer Nature, 2025. https://doi.org/10.1038/s44383-025-00002-8. ieee: A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” npj Science of Plants, vol. 1, no. 1. Springer Nature, p. 2, 2025. ista: Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2. mla: Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:10.1038/s44383-025-00002-8. short: A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2. corr_author: '1' date_created: 2026-02-03T13:03:53Z date_published: 2025-07-01T00:00:00Z date_updated: 2026-02-10T09:39:20Z day: '01' ddc: - '580' department: - _id: JiFr - _id: GradSch doi: 10.1038/s44383-025-00002-8 external_id: pmid: - '40630787' file: - access_level: open_access checksum: 6c190faacf0e3bef98311dc8a12132d4 content_type: application/pdf creator: dernst date_created: 2026-02-10T09:35:43Z date_updated: 2026-02-10T09:35:43Z file_id: '21208' file_name: 2025_NPJSciencePlants_Monzer.pdf file_size: 974106 relation: main_file success: 1 file_date_updated: 2026-02-10T09:35:43Z has_accepted_license: '1' intvolume: ' 1' issue: '1' language: - iso: eng month: '07' oa: 1 oa_version: Published Version page: '2' pmid: 1 project: - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants publication: npj Science of Plants publication_identifier: eissn: - 3005-1401 publication_status: published publisher: Springer Nature quality_controlled: '1' status: public title: Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 1 year: '2025' ... --- OA_type: closed access _id: '21255' abstract: - lang: eng text: As an important plant hormone to regulate growth and development, auxin has been investigated for more than a century. It had been clearly demonstrated and well-accepted that the intracellular auxin receptors, TIR1/AFBs, are F-box proteins mediating transcriptional auxin signaling by their E3 ubiquitin ligase activity, which targets and sends for degradation the Aux/IAA transcriptional repressors. The recent discovery of adenylate cyclase (AC) and guanylate cyclase (GC) activities for TIR1/AFBs open entirely new perspectives on how auxin signaling can operate. This chapter traces back the history of how canonical transcriptional auxin signaling was established and introduces the discovery of the TIR1/AFBs-mediated nontranscriptional signaling branch. Finally, the current understanding and open questions of how TIR1/AFBs’ AC and GC activities contribute to the transcriptional and nontranscriptional auxin signaling are discussed, highlighting the possibility that cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) act as second messengers in auxin signal transduction. alternative_title: - Foundations and Frontiers in Enzymology article_processing_charge: No author: - first_name: Linlin full_name: Qi, Linlin last_name: Qi - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: 'Qi L, Friml J. Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Irving H, Gehring C, Wong A, eds. Cryptic Enzymes and Moonlighting Proteins. Elsevier; 2025:299-322. doi:10.1016/b978-0-443-15719-6.00015-5' apa: 'Qi, L., & Friml, J. (2025). Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In H. Irving, C. Gehring, & A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins (pp. 299–322). Elsevier. https://doi.org/10.1016/b978-0-443-15719-6.00015-5' chicago: 'Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” In Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving, Chris Gehring, and Aloysius Wong, 299–322. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-15719-6.00015-5.' ieee: 'L. Qi and J. Friml, “Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling,” in Cryptic Enzymes and Moonlighting Proteins, H. Irving, C. Gehring, and A. Wong, Eds. Elsevier, 2025, pp. 299–322.' ista: 'Qi L, Friml J. 2025.Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Cryptic Enzymes and Moonlighting Proteins. Foundations and Frontiers in Enzymology, , 299–322.' mla: 'Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving et al., Elsevier, 2025, pp. 299–322, doi:10.1016/b978-0-443-15719-6.00015-5.' short: L. Qi, J. Friml, in:, H. Irving, C. Gehring, A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins, Elsevier, 2025, pp. 299–322. date_created: 2026-02-16T15:53:52Z date_published: 2025-05-02T00:00:00Z date_updated: 2026-02-17T13:28:38Z day: '02' department: - _id: JiFr doi: 10.1016/b978-0-443-15719-6.00015-5 editor: - first_name: Helen full_name: Irving, Helen last_name: Irving - first_name: Chris full_name: Gehring, Chris last_name: Gehring - first_name: Aloysius full_name: Wong, Aloysius last_name: Wong language: - iso: eng month: '05' oa_version: None page: 299-322 publication: Cryptic Enzymes and Moonlighting Proteins publication_identifier: isbn: - '9780443157196' publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: 'Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling' type: book_chapter user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '18619' abstract: - lang: eng text: Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signalling initiation, which requires binding of BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter, ABCB19, functions as a BR exporter, and together with its close homologue, ABCB1, positively regulate BR signalling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 was stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states were determined. In the BL-bound structure, BL was bound to the hydrophobic cavity formed by the transmembrane domain, and triggered local conformational changes. Together, our data provide additional insights into the ABC transporter-mediated BR export. acknowledgement: We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We thank Yaowei Wang, Yongming Luo, and Nemanja Vukašinović (VIB-UGhent, Belgium) for useful discussions and technical support. L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation. No conflict of interest is declared. article_number: '101181' article_processing_charge: Yes article_type: original author: - first_name: H full_name: Wei, H last_name: Wei - first_name: H full_name: Zhu, H last_name: Zhu - first_name: W full_name: Ying, W last_name: Ying - first_name: H full_name: Janssens, H last_name: Janssens - first_name: M full_name: Kvasnica, M last_name: Kvasnica - first_name: JM full_name: Winne, JM last_name: Winne - first_name: Y full_name: Gao, Y last_name: Gao - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Q full_name: Ma, Q last_name: Ma - first_name: S full_name: Tan, S last_name: Tan - first_name: X full_name: Liu, X last_name: Liu - first_name: E full_name: Russinova, E last_name: Russinova - first_name: L full_name: Sun, L last_name: Sun citation: ama: Wei H, Zhu H, Ying W, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 2025;6(1). doi:10.1016/j.xplc.2024.101181 apa: Wei, H., Zhu, H., Ying, W., Janssens, H., Kvasnica, M., Winne, J., … Sun, L. (2025). Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101181 chicago: Wei, H, H Zhu, W Ying, H Janssens, M Kvasnica, JM Winne, Y Gao, et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications. Elsevier, 2025. https://doi.org/10.1016/j.xplc.2024.101181. ieee: H. Wei et al., “Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1,” Plant Communications, vol. 6, no. 1. Elsevier, 2025. ista: Wei H, Zhu H, Ying W, Janssens H, Kvasnica M, Winne J, Gao Y, Friml J, Ma Q, Tan S, Liu X, Russinova E, Sun L. 2025. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 6(1), 101181. mla: Wei, H., et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications, vol. 6, no. 1, 101181, Elsevier, 2025, doi:10.1016/j.xplc.2024.101181. short: H. Wei, H. Zhu, W. Ying, H. Janssens, M. Kvasnica, J. Winne, Y. Gao, J. Friml, Q. Ma, S. Tan, X. Liu, E. Russinova, L. Sun, Plant Communications 6 (2025). date_created: 2024-12-04T11:21:16Z date_published: 2025-01-13T00:00:00Z date_updated: 2025-05-19T14:02:01Z day: '13' ddc: - '580' department: - _id: JiFr doi: 10.1016/j.xplc.2024.101181 external_id: isi: - '001416757300001' pmid: - '39497419' file: - access_level: open_access checksum: 7b0e4511e43cc0da06730c3edb7c1167 content_type: application/pdf creator: dernst date_created: 2025-04-16T09:02:05Z date_updated: 2025-04-16T09:02:05Z file_id: '19575' file_name: 2025_PlantComm_Wei.pdf file_size: 4443183 relation: main_file success: 1 file_date_updated: 2025-04-16T09:02:05Z has_accepted_license: '1' intvolume: ' 6' isi: 1 issue: '1' language: - iso: eng month: '01' oa: 1 oa_version: Published Version pmid: 1 publication: Plant Communications publication_identifier: issn: - 2590-3462 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1 tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 6 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '19003' abstract: - lang: eng text: 'Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ' acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: E-Lib - _id: M-Shop acknowledgement: "We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript." article_number: koaf006 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Michelle C full_name: Gallei, Michelle C id: 35A03822-F248-11E8-B48F-1D18A9856A87 last_name: Gallei orcid: 0000-0003-1286-7368 - first_name: Sven M full_name: Truckenbrodt, Sven M id: 45812BD4-F248-11E8-B48F-1D18A9856A87 last_name: Truckenbrodt - first_name: Caroline full_name: Kreuzinger, Caroline id: 382077BA-F248-11E8-B48F-1D18A9856A87 last_name: Kreuzinger - first_name: Syamala full_name: Inumella, Syamala id: F8660870-D756-11E9-98C5-34DFE5697425 last_name: Inumella orcid: 0009-0002-5890-120X - first_name: Vitali full_name: Vistunou, Vitali id: 7e146587-8972-11ed-ae7b-d7a32ea86a81 last_name: Vistunou - first_name: Christoph M full_name: Sommer, Christoph M id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87 last_name: Sommer orcid: 0000-0003-1216-9105 - first_name: Mojtaba full_name: Tavakoli, Mojtaba id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87 last_name: Tavakoli orcid: 0000-0002-7667-6854 - first_name: Nathalie full_name: Agudelo Duenas, Nathalie id: 40E7F008-F248-11E8-B48F-1D18A9856A87 last_name: Agudelo Duenas - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 - first_name: Wiebke full_name: Jahr, Wiebke id: 425C1CE8-F248-11E8-B48F-1D18A9856A87 last_name: Jahr orcid: 0000-0003-0201-2315 - first_name: Marek full_name: Randuch, Marek id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae last_name: Randuch - first_name: Alexander J full_name: Johnson, Alexander J id: 46A62C3A-F248-11E8-B48F-1D18A9856A87 last_name: Johnson orcid: 0000-0002-2739-8843 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 citation: ama: Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006 apa: Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006 chicago: Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University Press, 2025. https://doi.org/10.1093/plcell/koaf006. ieee: M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025. ista: Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006. mla: Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:10.1093/plcell/koaf006. short: M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025). corr_author: '1' date_created: 2025-02-05T06:52:06Z date_published: 2025-04-01T00:00:00Z date_updated: 2026-06-10T08:30:19Z day: '01' ddc: - '580' department: - _id: EvBe - _id: JoDa - _id: JiFr doi: 10.1093/plcell/koaf006 ec_funded: 1 external_id: isi: - '001462763100001' pmid: - '39792900' file: - access_level: open_access checksum: 9d3f8218ff37a29f29c48a7bbe831bd3 content_type: application/pdf creator: dernst date_created: 2025-07-31T07:03:43Z date_updated: 2025-07-31T07:03:43Z file_id: '20092' file_name: 2025_PlantCell_Gallei.pdf file_size: 53904111 relation: main_file success: 1 file_date_updated: 2025-07-31T07:03:43Z has_accepted_license: '1' intvolume: ' 37' isi: 1 issue: '4' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships - _id: 269B5B22-B435-11E9-9278-68D0E5697425 grant_number: ALTF 679-2018 name: UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED - _id: 6285a163-2b32-11ec-9570-8e204ca2dba5 grant_number: '26137' name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy - _id: 26AA4EF2-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232-B24 name: Molecular Drug Targets publication: The Plant Cell publication_identifier: eissn: - 1532-298X issn: - 1040-4651 publication_status: published publisher: Oxford University Press quality_controlled: '1' related_material: record: - id: '18689' relation: earlier_version status: public - id: '18837' relation: research_data status: public scopus_import: '1' status: public title: Super-resolution expansion microscopy in plant roots tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 37 year: '2025' ...