[{"project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805"}],"citation":{"apa":"Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher, C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell type diversity. bioRxiv. https://doi.org/10.64898/2026.02.12.705305","ama":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal cord cell type diversity. bioRxiv. doi:10.64898/2026.02.12.705305","short":"S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher, G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).","mla":"Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.” BioRxiv, doi:10.64898/2026.02.12.705305.","chicago":"Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B. Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” BioRxiv, n.d. https://doi.org/10.64898/2026.02.12.705305.","ista":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity. bioRxiv, 10.64898/2026.02.12.705305.","ieee":"S. A. Gobeil et al., “Lineage origin of spinal cord cell type diversity,” bioRxiv. ."},"month":"02","type":"preprint","oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"OA_place":"repository","date_updated":"2026-04-14T08:16:55Z","day":"16","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"oa_version":"Preprint","article_processing_charge":"No","date_published":"2026-02-16T00:00:00Z","status":"public","publication_status":"submitted","publication":"bioRxiv","abstract":[{"lang":"eng","text":"The complexity and specificity of movement in vertebrates is driven by a rich diversity of spinal motor and interneuron cell types. During development, eleven spinal cord progenitor domains generate an equivalent number of cardinal neuron types. How progenitor domains, individual progenitors, and post-mitotic diversity relate is still unknown. We performed high-resolution, single-progenitor cell lineage tracing in the embryonic mouse spinal cord using mosaic analysis with double markers (MADM). Our quantitative study of lineage progression revealed that spinal cord progenitors undergo highly variable numbers of proliferative, neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons migrate radially over large distances, span the dorsoventral axis, and even cross the midline, demonstrating striking bilaterality. Molecular and morphometric analysis indicate high levels of progenitor multipotency, with an individual progenitor capable of producing several molecularly and morphologically distinct neuron types, as well as astrocytes. These findings redefine spinal cord development as a process in which lineage variability — rather than rigid progenitor identity — drives the generation of cellular diversity."}],"ddc":["570"],"title":"Lineage origin of spinal cord cell type diversity","_id":"21291","doi":"10.64898/2026.02.12.705305","has_accepted_license":"1","acknowledgement":"We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko, and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB consortium for comments on\r\nthe manuscript. We are also grateful for the technical support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA). In addition, we thank our funding sources for providing\r\nthe resources to do these experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.); Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C. by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S. and G.C. were\r\nadditionally supported by institutional funds from the ISTA and the University of Exeter,\r\nrespectively. ","author":[{"id":"2f3e9efb-eb24-11ec-86b2-88efb11d59fa","last_name":"Gobeil","full_name":"Gobeil, Sophie A","first_name":"Sophie A"},{"full_name":"Da Silveira Neto, Francisco","id":"8cfb7412-10a7-11f1-add1-82b44e6418f2","last_name":"Da Silveira Neto","first_name":"Francisco"},{"first_name":"Giulia","full_name":"Silvestrelli, Giulia","id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9","last_name":"Silvestrelli"},{"first_name":"Matthijs Geert","full_name":"Smits, Matthijs Geert","last_name":"Smits","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0"},{"last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","first_name":"Carmen"},{"first_name":"Giselle T","orcid":"0000-0001-8457-2572","id":"471195F6-F248-11E8-B48F-1D18A9856A87","last_name":"Cheung","full_name":"Cheung, Giselle T"},{"full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","first_name":"Simon"},{"full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"}],"date_created":"2026-02-17T11:36:20Z","department":[{"_id":"SiHi"},{"_id":"LoSw"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.02.12.705305"}],"OA_type":"green","corr_author":"1"},{"external_id":{"pmid":["41964955 "]},"type":"journal_article","month":"04","quality_controlled":"1","OA_place":"publisher","intvolume":" 45","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"volume":45,"publisher":"Elsevier","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"},{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"}],"article_type":"original","file":[{"relation":"main_file","success":1,"date_updated":"2026-05-04T12:20:10Z","access_level":"open_access","file_size":14925958,"file_name":"2026_CellReports_Vijatovic.pdf","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef","file_id":"21795","creator":"dernst","content_type":"application/pdf","date_created":"2026-05-04T12:20:10Z"}],"citation":{"ieee":"D. Vijatovic et al., “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Elsevier, 2026.","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","chicago":"Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports. Elsevier, 2026. https://doi.org/10.1016/j.celrep.2026.117227.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227, Elsevier, 2026, doi:10.1016/j.celrep.2026.117227.","short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2026.117227"},"pmid":1,"abstract":[{"lang":"eng","text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution."}],"ddc":["570"],"title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","article_processing_charge":"Yes","date_published":"2026-04-28T00:00:00Z","date_updated":"2026-05-04T12:27:06Z","day":"28","oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication_status":"published","publication":"Cell Reports","status":"public","date_created":"2026-04-19T22:07:43Z","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"author":[{"full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic","first_name":"David"},{"id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","last_name":"Toma","full_name":"Toma, Florina Alexandra ","first_name":"Florina Alexandra "},{"first_name":"Y","last_name":"Ignatyev","full_name":"Ignatyev, Y"},{"id":"a8144562-32c9-11ee-b5ce-d9800628bda2","last_name":"Harrington","full_name":"Harrington, Zoe P","first_name":"Zoe P","orcid":"0009-0008-0158-4032"},{"full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"first_name":"Matthijs Geert","last_name":"Smits","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","full_name":"Smits, Matthijs Geert"},{"last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","full_name":"Dalla Vecchia, Marco","first_name":"Marco"},{"last_name":"Trevisan","full_name":"Trevisan, Alexandra J.","first_name":"Alexandra J."},{"full_name":"Chapman, Phillip","last_name":"Chapman","first_name":"Phillip"},{"full_name":"Julseth, Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","last_name":"Julseth","first_name":"Mara"},{"first_name":"Susan","full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"first_name":"Jeremy S.","full_name":"Dasen, Jeremy S.","last_name":"Dasen"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B.","first_name":"Jay B."},{"full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"}],"PlanS_conform":"1","doi":"10.1016/j.celrep.2026.117227","has_accepted_license":"1","DOAJ_listed":"1","_id":"21746","scopus_import":"1","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","issue":"4","language":[{"iso":"eng"}],"file_date_updated":"2026-05-04T12:20:10Z","OA_type":"gold","corr_author":"1","year":"2026","article_number":"117227","license":"https://creativecommons.org/licenses/by/4.0/","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"title":"Innovations in spinal cord cell type heterogeneity across vertebrate evolution","corr_author":"1","OA_type":"green","abstract":[{"lang":"eng","text":"Vertebrates display remarkable diversity of sensorimotor behaviors, each adapted to distinct ecological and survival demands. This diversity raises fundamental questions about the evolutionary origin of motor control: do conserved spinal circuits underlie these behaviors, and how have they diverged across species. Recent studies detail spinal cell-type architecture in mammals but comparable, high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we compare spinal cord cell types between fish, frogs, mice and humans, spanning ∼450 million years of evolution. Across species, we define highly conserved programs of cell type specification that segregate spinal neurons into nearly identical cardinal classes during development. This contrasts with adult stages, when spinal cell-type composition selectively diverges for excitatory neuron subpopulations. Using spatial transcriptomics, we localize this species divergence to the superficial, dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific cell types. The most dorsal spinal cord thus emerges as a recently evolved hub for sensory integration in mammals, a neospinal cord analogous to the neocortex."}],"language":[{"iso":"eng"}],"main_file_link":[{"url":"https://doi.org/10.1101/2025.10.09.680955","open_access":"1"}],"publication_status":"submitted","publication":"bioRxiv","status":"public","article_processing_charge":"No","date_published":"2025-10-11T00:00:00Z","year":"2025","oa_version":"Preprint","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","day":"11","date_updated":"2026-05-27T07:25:41Z","OA_place":"repository","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"department":[{"_id":"LoSw"},{"_id":"ScienComp"}],"date_created":"2026-05-27T06:54:04Z","month":"10","type":"preprint","author":[{"last_name":"Ignatyev","full_name":"Ignatyev, Yuri","first_name":"Yuri"},{"first_name":"Stavros","full_name":"Papadopoulos, Stavros","id":"40606b92-f128-11eb-9611-bf66a98cfa5c","last_name":"Papadopoulos"},{"full_name":"Soretić, Mateja","last_name":"Soretić","first_name":"Mateja"},{"last_name":"Yeung","id":"123012b2-db30-11eb-b4d8-a35840c0551b","full_name":"Yeung, Jake","first_name":"Jake","orcid":"0000-0003-1732-1559"},{"first_name":"Tzi-Yang","full_name":"Lin, Tzi-Yang","last_name":"Lin"},{"first_name":"Elly M","full_name":"Tanaka, Elly M","last_name":"Tanaka"},{"first_name":"Leonid","full_name":"Peshkin, Leonid","last_name":"Peshkin"},{"first_name":"Ariel J","last_name":"Levine","full_name":"Levine, Ariel J"},{"full_name":"Gabitto, Mariano I","last_name":"Gabitto","first_name":"Mariano I"},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger"}],"citation":{"apa":"Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka, E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. https://doi.org/10.1101/2025.10.09.680955","ama":"Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. doi:10.1101/2025.10.09.680955","mla":"Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, doi:10.1101/2025.10.09.680955.","short":"Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka, L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.).","ieee":"Y. Ignatyev et al., “Innovations in spinal cord cell type heterogeneity across vertebrate evolution,” bioRxiv. .","ista":"Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv, 10.1101/2025.10.09.680955.","chicago":"Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.09.680955."},"acknowledgement":"We would like to thank the members of the Sweeney Lab for discussion and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions of the NIH author are considered Works of the United States\r\nGovernment. The findings and conclusions presented in this paper are those of\r\nthe authors and do not necessarily reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. ","project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis","grant_number":"27229","_id":"907b765e-16d5-11f0-9cad-fef108a945b1"}],"doi":"10.1101/2025.10.09.680955","_id":"21920"},{"language":[{"iso":"eng"}],"file_date_updated":"2025-06-04T05:43:27Z","corr_author":"1","OA_type":"hybrid","year":"2025","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-02-20T09:20:32Z","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"author":[{"first_name":"Eliza C.B.","last_name":"Jaeger","full_name":"Jaeger, Eliza C.B."},{"full_name":"Vijatovic, David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58","first_name":"David"},{"last_name":"Deryckere","full_name":"Deryckere, Astrid","first_name":"Astrid"},{"last_name":"Zorin","full_name":"Zorin, Nikol","first_name":"Nikol"},{"full_name":"Nguyen, Akemi L.","last_name":"Nguyen","first_name":"Akemi L."},{"first_name":"Georgiy","last_name":"Ivanian","id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","full_name":"Ivanian, Georgiy"},{"first_name":"Jamie","last_name":"Woych","full_name":"Woych, Jamie"},{"first_name":"Rebecca C","full_name":"Arnold, Rebecca C","last_name":"Arnold","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f"},{"first_name":"Alonso","full_name":"Ortega Gurrola, Alonso","last_name":"Ortega Gurrola"},{"first_name":"Arik","full_name":"Shvartsman, Arik","last_name":"Shvartsman"},{"first_name":"Francesca","last_name":"Barbieri","id":"a9492887-8972-11ed-ae7b-bfae10998254","full_name":"Barbieri, Francesca"},{"first_name":"Florina-Alexandra","last_name":"Toma","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5","full_name":"Toma, Florina-Alexandra"},{"full_name":"Gorbsky, Gary J.","last_name":"Gorbsky","first_name":"Gary J."},{"first_name":"Marko E.","last_name":"Horb","full_name":"Horb, Marko E."},{"last_name":"Cline","full_name":"Cline, Hollis T.","first_name":"Hollis T."},{"first_name":"Timothy F.","full_name":"Shay, Timothy F.","last_name":"Shay"},{"first_name":"Darcy B.","full_name":"Kelley, Darcy B.","last_name":"Kelley"},{"first_name":"Ayako","last_name":"Yamaguchi","full_name":"Yamaguchi, Ayako"},{"first_name":"Mark","last_name":"Shein-Idelson","full_name":"Shein-Idelson, Mark"},{"last_name":"Tosches","full_name":"Tosches, Maria Antonietta","first_name":"Maria Antonietta"},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger"}],"has_accepted_license":"1","doi":"10.1016/j.devcel.2024.10.025","_id":"15016","scopus_import":"1","acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","issue":"5","abstract":[{"lang":"eng","text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution."}],"ddc":["570"],"title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","article_processing_charge":"Yes (via OA deal)","date_published":"2025-03-10T00:00:00Z","date_updated":"2025-09-30T10:00:55Z","day":"10","oa_version":"Published Version","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"publication":"Developmental Cell","publication_status":"published","status":"public","external_id":{"isi":["001444798600001"],"pmid":["39603234"]},"type":"journal_article","month":"03","page":"794-812.e6","quality_controlled":"1","OA_place":"publisher","intvolume":" 60","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":60,"publisher":"Elsevier","oa":1,"project":[{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"},{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"}],"article_type":"original","file":[{"success":1,"relation":"main_file","date_updated":"2025-06-04T05:43:27Z","access_level":"open_access","file_size":11936258,"file_id":"19790","checksum":"a83a4cb58f5941096d3ad91ca0172594","file_name":"2025_DevelopmentalCell_Jaeger.pdf","content_type":"application/pdf","date_created":"2025-06-04T05:43:27Z","creator":"dernst"}],"citation":{"apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.10.025","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 2025;60(5):794-812.e6. doi:10.1016/j.devcel.2024.10.025","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” Developmental Cell, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:10.1016/j.devcel.2024.10.025.","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.025.","ieee":"E. C. B. Jaeger et al., “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” Developmental Cell, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025."},"pmid":1},{"quality_controlled":"1","external_id":{"isi":["001426697000031"],"pmid":["39972051"]},"month":"02","type":"journal_article","page":"6059","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Springer Nature","oa":1,"volume":15,"intvolume":" 15","OA_place":"publisher","publication_identifier":{"eissn":["2045-2322"]},"article_type":"original","file":[{"success":1,"relation":"main_file","access_level":"open_access","file_size":2780316,"date_updated":"2025-03-10T12:00:34Z","file_id":"19380","checksum":"51b55ae299de1fa126016a11024b499a","file_name":"2025_ScientificReports_SaenzdeJuano.pdf","date_created":"2025-03-10T12:00:34Z","content_type":"application/pdf","creator":"dernst"}],"pmid":1,"citation":{"ista":"Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich SE. 2025. Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific Reports. 15, 6059.","chicago":"Saenz-De-Juano, Mara D., Giulia Silvestrelli, Samuel Buri, Léa V. Zinsli, Mathias Schmelcher, and Susanne E. Ulbrich. “Mastitis-Related Staphylococcus Aureus-Derived Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived Macrophages.” Scientific Reports. Springer Nature, 2025. https://doi.org/10.1038/s41598-025-90466-6.","ieee":"M. D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L. V. Zinsli, M. Schmelcher, and S. E. Ulbrich, “Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages,” Scientific Reports, vol. 15. Springer Nature, p. 6059, 2025.","short":"M.D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L.V. Zinsli, M. Schmelcher, S.E. Ulbrich, Scientific Reports 15 (2025) 6059.","mla":"Saenz-De-Juano, Mara D., et al. “Mastitis-Related Staphylococcus Aureus-Derived Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived Macrophages.” Scientific Reports, vol. 15, Springer Nature, 2025, p. 6059, doi:10.1038/s41598-025-90466-6.","ama":"Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich SE. Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific Reports. 2025;15:6059. doi:10.1038/s41598-025-90466-6","apa":"Saenz-De-Juano, M. D., Silvestrelli, G., Buri, S., Zinsli, L. V., Schmelcher, M., & Ulbrich, S. E. (2025). Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-025-90466-6"},"abstract":[{"lang":"eng","text":"Staphylococcus aureus (S. aureus) is one of the most common causative agents of mammary gland infection and mastitis, but the specific role of S. aureus-derived extracellular vesicles (SaEVs) in mastitis has been poorly studied to date. Here, we aimed to investigate the response of bovine monocyte-derived macrophages (boMdM) to SaEVs of the genotype B (GTB) mastitis-related strain M5512B. Specifically, we evaluated the effects on the actin cytoskeleton, gene expression, and the SaEV proteomic cargo. Furthermore, we assessed to what extent the cellular and molecular response of boMdM to SaEVs differed from peripheral mononuclear blood cells (PBMCs) used for in vitro derivation of the former. We observed that SaEVs induced morphological changes in boMdM, leading to a pro-inflammatory and pyroptosis-related increased gene expression. Additionally, our study revealed that boMdM and PBMCs exhibited stimulus-specific differing responses. The proteomic analysis of SaEVs identified clusters of proteins related to virulence and antibiotic resistance, supporting the theory that S. aureus might use EVs to evade host defences and colonize the mammary gland. Our results bring new insights into how SaEVs might impact the host during an S. aureus infection, which can be useful for future S. aureus vaccine development."}],"ddc":["570"],"title":"Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages","date_updated":"2025-09-30T10:58:59Z","day":"19","oa_version":"Published Version","date_published":"2025-02-19T00:00:00Z","article_processing_charge":"Yes","status":"public","publication":"Scientific Reports","publication_status":"published","author":[{"first_name":"Mara D.","full_name":"Saenz-De-Juano, Mara D.","last_name":"Saenz-De-Juano"},{"first_name":"Giulia","full_name":"Silvestrelli, Giulia","id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9","last_name":"Silvestrelli"},{"full_name":"Buri, Samuel","last_name":"Buri","first_name":"Samuel"},{"first_name":"Léa V.","full_name":"Zinsli, Léa V.","last_name":"Zinsli"},{"first_name":"Mathias","full_name":"Schmelcher, Mathias","last_name":"Schmelcher"},{"first_name":"Susanne E.","last_name":"Ulbrich","full_name":"Ulbrich, Susanne E."}],"department":[{"_id":"LoSw"}],"date_created":"2025-03-09T23:01:26Z","DOAJ_listed":"1","_id":"19366","doi":"10.1038/s41598-025-90466-6","has_accepted_license":"1","acknowledgement":"The authors thank Michele Guastalla for his contributions to the boMdM analyses and Stephan Handschin from the Scientific Center for Optical and Electron Microscopy (ScopeM) of ETH Zurich for the TEM imaging. We gratefully acknowledge the Functional Genomics Center Zurich (FGCZ) for performing the mass spectrometry analysis for this study.\r\nOpen access funding provided by Swiss Federal Institute of Technology Zurich. This work was supported by basic funding from ETH Zurich.","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"gold","file_date_updated":"2025-03-10T12:00:34Z","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2025"},{"doi":"10.15479/AT-ISTA-20735","has_accepted_license":"1","_id":"20735","department":[{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"LoSw"}],"date_created":"2025-12-08T09:49:41Z","author":[{"last_name":"Wilson","id":"5230e794-15b2-11ec-abd3-e2d5335ebd1d","full_name":"Wilson, Alexia C","first_name":"Alexia C","orcid":"0000-0001-6191-1367"}],"year":"2025","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"13097"}]},"supervisor":[{"orcid":"0000-0003-3295-6181","first_name":"Tim P","full_name":"Vogels, Tim P","last_name":"Vogels","id":"CB6FF8D2-008F-11EA-8E08-2637E6697425"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","degree_awarded":"MS","language":[{"iso":"eng"}],"file_date_updated":"2026-01-04T12:58:49Z","corr_author":"1","citation":{"short":"A.C. Wilson, Modelling the Spinal Cord of a Tadpole: Exploring Different Ways to Model the Spinal Cord in the Xenopus Frog, Institute of Science and Technology Austria, 2025.","mla":"Wilson, Alexia C. Modelling the Spinal Cord of a Tadpole: Exploring Different Ways to Model the Spinal Cord in the Xenopus Frog. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20735.","ista":"Wilson AC. 2025. Modelling the spinal cord of a tadpole: Exploring different ways to model the spinal cord in the Xenopus frog. Institute of Science and Technology Austria.","chicago":"Wilson, Alexia C. “Modelling the Spinal Cord of a Tadpole: Exploring Different Ways to Model the Spinal Cord in the Xenopus Frog.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20735.","ieee":"A. C. Wilson, “Modelling the spinal cord of a tadpole: Exploring different ways to model the spinal cord in the Xenopus frog,” Institute of Science and Technology Austria, 2025.","apa":"Wilson, A. C. (2025). Modelling the spinal cord of a tadpole: Exploring different ways to model the spinal cord in the Xenopus frog. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20735","ama":"Wilson AC. Modelling the spinal cord of a tadpole: Exploring different ways to model the spinal cord in the Xenopus frog. 2025. doi:10.15479/AT-ISTA-20735"},"file":[{"access_level":"closed","file_size":566072368,"date_updated":"2026-01-02T13:05:07Z","relation":"source_file","creator":"awilson","content_type":"application/zip","date_created":"2026-01-01T17:26:30Z","checksum":"9e3b6b73f8cbec2c3687d17fe8e30410","file_name":"tadpoleAdEx.zip","file_id":"20919"},{"success":1,"relation":"main_file","file_size":7170097,"access_level":"open_access","date_updated":"2026-01-04T12:58:49Z","file_id":"20923","checksum":"13f4c0d33923e9d5c9d56731345cf21d","file_name":"Masters_Thesis_Alexia_Wilson_FINAL_pdfA.pdf","content_type":"application/pdf","date_created":"2026-01-04T12:58:49Z","creator":"awilson"}],"page":"110","month":"12","type":"dissertation","publication_identifier":{"issn":["2791-4585"]},"OA_place":"publisher","publisher":"Institute of Science and Technology Austria","oa":1,"article_processing_charge":"No","date_published":"2025-12-09T00:00:00Z","oa_version":"Published Version","alternative_title":["ISTA Master's Thesis"],"day":"09","date_updated":"2026-07-29T12:55:12Z","status":"public","publication_status":"published","doi_confirm":"1","abstract":[{"text":"Left–right alternation is a defining feature of spinal locomotor circuits, yet the level of neuronal\r\ndetail required to generate and maintain this pattern remains unclear. This thesis investigates how\r\nmodels spanning multiple levels of abstraction—from biophysically detailed Hodgkin–Huxley (HH)\r\nneurons to adaptive integrate–and–fire (I&F) formulations and synfire-chain modules—can account\r\nfor the generation of fictive swimming in the spinal cord of the Xenopus laevis tadpole. The guiding\r\nhypothesis is that a small set of neuronal mechanisms is sufficient to reproduce the essential features\r\nof rhythmic alternation, and that moving between modeling scales helps distinguish core principles\r\nfrom biological detail.\r\nA minimal bilateral HH network comprising only four canonical neuron classes—excitatory\r\ndescending interneurons (dINs), inhibitory commissural interneurons (cINs), ipsilateral inhibitory\r\ninterneurons (aINs) and motoneurons—served as a biophysical proof of concept. Tuned to reproduce\r\nexperimentally observed firing modes, the model demonstrated that rebound-prone dIN excitability,\r\ncontralateral inhibition and modest electrical coupling are sufficient to generate stable alternating\r\nactivity, even in very small networks. These results motivated the transition to simpler models\r\ncapable of efficient analysis and scaling.\r\nAdaptive exponential I&F (AdEx) neurons were calibrated to physiological recordings using\r\nsimulation-based inference, yielding tonic and phasic/rebound templates that preserved the key\r\ndynamical signatures of the HH model. Phase-plane analysis clarified the mechanisms underlying\r\nsingle-spike responses and rebound firing in dINs. At network level, the I&F models robustly\r\nreproduced left–right alternation, while highlighting constraints on synaptic kinetics and adaptation\r\nneeded to avoid multi-spike responses.\r\nFinally, a synfire-chain framework provided a complementary, timing-centric perspective, demonstrating how precise spike synchrony, synaptic delays and minimal inhibitory coupling can generate\r\nalternating left–right sequences in a feedforward setting. Together, these approaches converge on a\r\ncommon conclusion: rebound-prone ipsilateral excitation combined with precisely timed contralateral inhibition constitutes a sufficient substrate for alternating spinal rhythms.\r\nBy integrating bottom-up and top-down modeling strategies, this thesis provides a unified, extensible framework for studying spinal pattern generation. The results show that essential locomotor\r\ndynamics can be captured across multiple abstraction levels, offering both mechanistic insight and\r\npractical tools for future data-driven investigations of spinal circuit development, robustness and\r\nmodulation.","lang":"eng"}],"title":"Modelling the spinal cord of a tadpole: Exploring different ways to model the spinal cord in the Xenopus frog","ddc":["570","596","005"]},{"_id":"15335","has_accepted_license":"1","doi":"10.1073/pnas.2318041121","acknowledgement":"We thank all members of the Spitzer laboratory for discussions and critical feedback; K. Marek for discussions of acknowledgment signals; I. Gregor and R. Aricescu for discussions of receptor pharmacology and transsynaptic bridges; C. Kintner for advice on Xenopus blastomere lineage; A. Ray and E. Park for guidance on miniature analysis; A. Glavis- Bloom, S.U. Choi, S. Atkins, M. Gupta, and S. Malladi for technical assistance; and D. K. Berg and L. R. Squire for comments on the manuscript. This work was supported by NSF 2051555 and the Overland Foundation. Microscopy for five- channel imaging utilized the UCSD School of Medicine Microscopy Core, supported by NIH grant NS047101.","issue":"15","scopus_import":"1","author":[{"first_name":"Swetha K.","last_name":"Godavarthi","full_name":"Godavarthi, Swetha K."},{"first_name":"Masaki","last_name":"Hiramoto","full_name":"Hiramoto, Masaki"},{"last_name":"Ignatyev","full_name":"Ignatyev, Yuri","first_name":"Yuri"},{"first_name":"Jacqueline B.","last_name":"Levin","full_name":"Levin, Jacqueline B."},{"first_name":"Hui Quan","full_name":"Li, Hui Quan","last_name":"Li"},{"first_name":"Marta","last_name":"Pratelli","full_name":"Pratelli, Marta"},{"first_name":"Jennifer","last_name":"Borchardt","full_name":"Borchardt, Jennifer"},{"first_name":"Cynthia","full_name":"Czajkowski, Cynthia","last_name":"Czajkowski"},{"full_name":"Borodinsky, Laura N.","last_name":"Borodinsky","first_name":"Laura N."},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger"},{"first_name":"Hollis T.","full_name":"Cline, Hollis T.","last_name":"Cline"},{"last_name":"Spitzer","full_name":"Spitzer, Nicholas C.","first_name":"Nicholas C."}],"department":[{"_id":"LoSw"}],"date_created":"2024-04-21T22:00:53Z","isi":1,"article_number":"e2318041121","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2024","language":[{"iso":"eng"}],"file_date_updated":"2024-04-23T06:53:14Z","article_type":"original","file":[{"relation":"main_file","success":1,"date_updated":"2024-04-23T06:53:14Z","access_level":"open_access","file_size":16187094,"file_name":"2024_PNAS_Godavarthi.pdf","checksum":"f3b4ffad4ef3d1c443414edf0cd2392c","file_id":"15340","creator":"dernst","date_created":"2024-04-23T06:53:14Z","content_type":"application/pdf"}],"citation":{"short":"S.K. Godavarthi, M. Hiramoto, Y. Ignatyev, J.B. Levin, H.Q. Li, M. Pratelli, J. Borchardt, C. Czajkowski, L.N. Borodinsky, L.B. Sweeney, H.T. Cline, N.C. Spitzer, Proceedings of the National Academy of Sciences of the United States of America 121 (2024).","mla":"Godavarthi, Swetha K., et al. “Postsynaptic Receptors Regulate Presynaptic Transmitter Stability through Transsynaptic Bridges.” Proceedings of the National Academy of Sciences of the United States of America, vol. 121, no. 15, e2318041121, National Academy of Sciences, 2024, doi:10.1073/pnas.2318041121.","ista":"Godavarthi SK, Hiramoto M, Ignatyev Y, Levin JB, Li HQ, Pratelli M, Borchardt J, Czajkowski C, Borodinsky LN, Sweeney LB, Cline HT, Spitzer NC. 2024. Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. Proceedings of the National Academy of Sciences of the United States of America. 121(15), e2318041121.","chicago":"Godavarthi, Swetha K., Masaki Hiramoto, Yuri Ignatyev, Jacqueline B. Levin, Hui Quan Li, Marta Pratelli, Jennifer Borchardt, et al. “Postsynaptic Receptors Regulate Presynaptic Transmitter Stability through Transsynaptic Bridges.” Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences, 2024. https://doi.org/10.1073/pnas.2318041121.","ieee":"S. K. Godavarthi et al., “Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges,” Proceedings of the National Academy of Sciences of the United States of America, vol. 121, no. 15. National Academy of Sciences, 2024.","apa":"Godavarthi, S. K., Hiramoto, M., Ignatyev, Y., Levin, J. B., Li, H. Q., Pratelli, M., … Spitzer, N. C. (2024). Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.2318041121","ama":"Godavarthi SK, Hiramoto M, Ignatyev Y, et al. Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. Proceedings of the National Academy of Sciences of the United States of America. 2024;121(15). doi:10.1073/pnas.2318041121"},"pmid":1,"quality_controlled":"1","external_id":{"isi":["001243892800004"],"pmid":["38568976"]},"month":"04","type":"journal_article","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"publisher":"National Academy of Sciences","volume":121,"intvolume":" 121","publication_identifier":{"eissn":["1091-6490"]},"date_updated":"2025-09-04T13:42:01Z","day":"09","oa_version":"Published Version","article_processing_charge":"Yes (in subscription journal)","date_published":"2024-04-09T00:00:00Z","status":"public","publication":"Proceedings of the National Academy of Sciences of the United States of America","publication_status":"published","abstract":[{"text":"Stable matching of neurotransmitters with their receptors is fundamental to synapse function and reliable communication in neural circuits. Presynaptic neurotransmitters regulate the stabilization of postsynaptic transmitter receptors. Whether postsynaptic receptors regulate stabilization of presynaptic transmitters has received less attention. Here, we show that blockade of endogenous postsynaptic acetylcholine receptors (AChR) at the neuromuscular junction destabilizes the cholinergic phenotype in motor neurons and stabilizes an earlier, developmentally transient glutamatergic phenotype. Further, expression of exogenous postsynaptic gamma-aminobutyric acid type A receptors (GABAA receptors) in muscle cells stabilizes an earlier, developmentally transient GABAergic motor neuron phenotype. Both AChR and GABAA receptors are linked to presynaptic neurons through transsynaptic bridges. Knockdown of specific components of these transsynaptic bridges prevents stabilization of the cholinergic or GABAergic phenotypes. Bidirectional communication can enforce a match between transmitter and receptor and ensure the fidelity of synaptic transmission. Our findings suggest a potential role of dysfunctional transmitter receptors in neurological disorders that involve the loss of the presynaptic transmitter.","lang":"eng"}],"ddc":["570"],"title":"Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges"},{"abstract":[{"text":"Vertebrates exhibit a wide range of motor behaviors, ranging from swimming to complex limb-based movements. Here we take advantage of frog metamorphosis, which captures a swim-to-limb-based movement transformation during the development of a single organism, to explore changes in the underlying spinal circuits. We find that the tadpole spinal cord contains small and largely homogeneous populations of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages. These neuronal populations only modestly increase in number and subtype heterogeneity with the emergence of free swimming. In contrast, during frog metamorphosis and the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron number and transcriptional heterogeneity, culminating in cohorts of neurons that exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively, results in severe but selective deficits in tail and limb function. Our work thus demonstrates that neural diversity scales exponentially with increasing behavioral complexity and illustrates striking evolutionary conservation in the molecular organization and function of motor circuits across species.","lang":"eng"}],"language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.09.20.614050"}],"OA_type":"green","corr_author":"1","title":"Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis","acknowledged_ssus":[{"_id":"Bio"}],"oa_version":"Preprint","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"27","date_updated":"2025-05-14T11:40:13Z","date_published":"2024-09-27T00:00:00Z","article_processing_charge":"No","year":"2024","status":"public","publication_status":"submitted","publication":"bioRxiv","author":[{"first_name":"David","full_name":"Vijatovic, David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"first_name":"Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","last_name":"Toma","full_name":"Toma, Florina Alexandra "},{"last_name":"Harrington","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","full_name":"Harrington, Zoe P","first_name":"Zoe P","orcid":"0009-0008-0158-4032"},{"last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","first_name":"Christoph M","orcid":"0000-0003-1216-9105"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"last_name":"Trevisan","full_name":"Trevisan, Alexandra J.","first_name":"Alexandra J."},{"first_name":"Phillip","last_name":"Chapman","full_name":"Chapman, Phillip"},{"first_name":"Mara","full_name":"Julseth, Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"last_name":"Brenner-Morton","full_name":"Brenner-Morton, Susan","first_name":"Susan"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"first_name":"Jeremy S.","full_name":"Dasen, Jeremy S.","last_name":"Dasen"},{"first_name":"Jay B.","full_name":"Bikoff, Jay B.","last_name":"Bikoff"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney"}],"department":[{"_id":"LoSw"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"date_created":"2025-04-07T08:48:28Z","type":"preprint","month":"09","oa":1,"OA_place":"repository","_id":"19520","doi":"10.1101/2024.09.20.614050","project":[{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","grant_number":"CZI01"}],"citation":{"mla":"Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, doi:10.1101/2024.09.20.614050.","short":"D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).","ieee":"D. Vijatovic et al., “Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis,” bioRxiv. .","ista":"Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv, 10.1101/2024.09.20.614050.","chicago":"Vijatovic, David, Florina Alexandra Toma, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, n.d. https://doi.org/10.1101/2024.09.20.614050.","apa":"Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R., Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv. https://doi.org/10.1101/2024.09.20.614050","ama":"Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv. doi:10.1101/2024.09.20.614050"},"acknowledgement":"We would like to thank the members of the Sweeney Lab (especially Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion, and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their scientific insight and mentorship during the conception of this project. This\r\nproject would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA). In addition, we thank our funding sources for providing the resources to do these\r\nexperiments: FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS 5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.); NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities (ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472 (Z.H.); and Project A.L.S. (S.B-M.). "},{"title":"Molecular and cellular mechanisms of motor circuit development","ddc":["570"],"abstract":[{"text":"Motor circuits represent the main output of the central nervous system and produce dynamic behaviors ranging from relatively simple rhythmic activities like swimming in fish and breathing in mammals to highly sophisticated dexterous movements in humans. Despite decades of research, the development and function of motor circuits remain poorly understood. Breakthroughs in the field recently provided new tools and tractable model systems that set the stage to discover the molecular mechanisms and circuit logic underlying motor control. Here, we describe recent advances from both vertebrate (mouse, frog) and invertebrate (nematode, fruit fly) systems on cellular and molecular mechanisms that enable motor circuits to develop and function and highlight conserved and divergent mechanisms necessary for motor circuit development.","lang":"eng"}],"publication":"The Journal of Neuroscience","status":"public","publication_status":"published","article_processing_charge":"No","date_published":"2024-10-02T00:00:00Z","oa_version":"Published Version","day":"02","date_updated":"2026-01-05T14:01:26Z","publication_identifier":{"eissn":["1529-2401"]},"intvolume":" 44","OA_place":"publisher","publisher":"Society for Neuroscience","oa":1,"OA_embargo":"6 months","volume":44,"month":"10","type":"journal_article","external_id":{"pmid":["39358025"],"isi":["001335212200016"]},"quality_controlled":"1","citation":{"apa":"Kratsios, P., Zampieri, N., Carrillo, R., Mizumoto, K., Sweeney, L. B., & Philippidou, P. (2024). Molecular and cellular mechanisms of motor circuit development. The Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.1238-24.2024","ama":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. Molecular and cellular mechanisms of motor circuit development. The Journal of Neuroscience. 2024;44(40). doi:10.1523/JNEUROSCI.1238-24.2024","short":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L.B. Sweeney, P. Philippidou, The Journal of Neuroscience 44 (2024).","mla":"Kratsios, Paschalis, et al. “Molecular and Cellular Mechanisms of Motor Circuit Development.” The Journal of Neuroscience, vol. 44, no. 40, e1238242024, Society for Neuroscience, 2024, doi:10.1523/JNEUROSCI.1238-24.2024.","chicago":"Kratsios, Paschalis, Niccolò Zampieri, Robert Carrillo, Kota Mizumoto, Lora B. Sweeney, and Polyxeni Philippidou. “Molecular and Cellular Mechanisms of Motor Circuit Development.” The Journal of Neuroscience. Society for Neuroscience, 2024. https://doi.org/10.1523/JNEUROSCI.1238-24.2024.","ista":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. 2024. Molecular and cellular mechanisms of motor circuit development. The Journal of Neuroscience. 44(40), e1238242024.","ieee":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L. B. Sweeney, and P. Philippidou, “Molecular and cellular mechanisms of motor circuit development,” The Journal of Neuroscience, vol. 44, no. 40. Society for Neuroscience, 2024."},"pmid":1,"article_type":"original","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"}],"OA_type":"hybrid","main_file_link":[{"url":"https://doi.org/10.1523/JNEUROSCI.1238-24.2024","open_access":"1"}],"language":[{"iso":"eng"}],"year":"2024","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"e1238242024","isi":1,"department":[{"_id":"LoSw"}],"date_created":"2024-10-13T22:01:49Z","author":[{"last_name":"Kratsios","full_name":"Kratsios, Paschalis","first_name":"Paschalis"},{"first_name":"Niccolò","full_name":"Zampieri, Niccolò","last_name":"Zampieri"},{"last_name":"Carrillo","full_name":"Carrillo, Robert","first_name":"Robert"},{"first_name":"Kota","last_name":"Mizumoto","full_name":"Mizumoto, Kota"},{"first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger"},{"first_name":"Polyxeni","last_name":"Philippidou","full_name":"Philippidou, Polyxeni"}],"scopus_import":"1","issue":"40","acknowledgement":"Work in the authors’ labs is funded by the Helmholtz Association (N.Z.), National Institute of Neurological Disorders and Stroke (NINDS) R01NS116365 (P.K.), NINDS R01NS123439 and National Science Foundation IOS-2048080 (R.C.), NINDS R01NS114510 (P.P.), Natural Sciences and Engineering Research Council of Canada RGPIN-2021-03154 (K.M.) and Horizon Europe European Research Council Starting Grant Number 101041551 (L.B.S.). P.P. is the Weidenthal Family Designated Professor in Career Development.","doi":"10.1523/JNEUROSCI.1238-24.2024","has_accepted_license":"1","_id":"18305"},{"article_processing_charge":"Yes","date_published":"2023-04-26T00:00:00Z","date_updated":"2026-07-29T12:55:11Z","day":"26","oa_version":"Published Version","status":"public","publication_status":"published","publication":"Frontiers in Neural Circuits","abstract":[{"lang":"eng","text":"Vertebrate movement is orchestrated by spinal inter- and motor neurons that, together with sensory and cognitive input, produce dynamic motor behaviors. These behaviors vary from the simple undulatory swimming of fish and larval aquatic species to the highly coordinated running, reaching and grasping of mice, humans and other mammals. This variation raises the fundamental question of how spinal circuits have changed in register with motor behavior. In simple, undulatory fish, exemplified by the lamprey, two broad classes of interneurons shape motor neuron output: ipsilateral-projecting excitatory neurons, and commissural-projecting inhibitory neurons. An additional class of ipsilateral inhibitory neurons is required to generate escape swim behavior in larval zebrafish and tadpoles. In limbed vertebrates, a more complex spinal neuron composition is observed. In this review, we provide evidence that movement elaboration correlates with an increase and specialization of these three basic interneuron types into molecularly, anatomically, and functionally distinct subpopulations. We summarize recent work linking neuron types to movement-pattern generation across fish, amphibians, reptiles, birds and mammals."}],"ddc":["570"],"title":"Spinal cords: Symphonies of interneurons across species","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"}],"article_type":"original","file":[{"content_type":"application/pdf","date_created":"2024-01-03T13:33:21Z","creator":"dernst","file_id":"14729","file_name":"2023_FrontiersNeuralCircuits_Wilson.pdf","checksum":"7efd06de284a28e91e97127611a9c3fd","file_size":6667157,"access_level":"open_access","date_updated":"2024-01-03T13:33:21Z","success":1,"relation":"main_file"}],"citation":{"apa":"Wilson, A. C., & Sweeney, L. B. (2023). Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. Frontiers. https://doi.org/10.3389/fncir.2023.1146449","ama":"Wilson AC, Sweeney LB. Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. 2023;17. doi:10.3389/fncir.2023.1146449","short":"A.C. Wilson, L.B. Sweeney, Frontiers in Neural Circuits 17 (2023).","mla":"Wilson, Alexia C., and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” Frontiers in Neural Circuits, vol. 17, 1146449, Frontiers, 2023, doi:10.3389/fncir.2023.1146449.","chicago":"Wilson, Alexia C, and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” Frontiers in Neural Circuits. Frontiers, 2023. https://doi.org/10.3389/fncir.2023.1146449.","ista":"Wilson AC, Sweeney LB. 2023. Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. 17, 1146449.","ieee":"A. C. Wilson and L. B. Sweeney, “Spinal cords: Symphonies of interneurons across species,” Frontiers in Neural Circuits, vol. 17. Frontiers, 2023."},"pmid":1,"month":"04","type":"journal_article","external_id":{"pmid":["37180760"],"isi":["000984606200001"]},"quality_controlled":"1","intvolume":" 17","publication_identifier":{"issn":["1662-5110"]},"oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":17,"publisher":"Frontiers","related_material":{"record":[{"status":"public","id":"20735","relation":"dissertation_contains"}]},"year":"2023","isi":1,"article_number":"1146449","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"file_date_updated":"2024-01-03T13:33:21Z","corr_author":"1","doi":"10.3389/fncir.2023.1146449","has_accepted_license":"1","_id":"13097","scopus_import":"1","acknowledgement":"This work was supported by the ERC Starting grant, ERC-2021-STG #101041551.","date_created":"2023-05-28T22:01:04Z","department":[{"_id":"LoSw"}],"author":[{"id":"5230e794-15b2-11ec-abd3-e2d5335ebd1d","last_name":"Wilson","full_name":"Wilson, Alexia C","first_name":"Alexia C","orcid":"0000-0001-6191-1367"},{"last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}]},{"department":[{"_id":"DaSi"},{"_id":"LoSw"}],"date_created":"2022-03-24T13:23:09Z","author":[{"first_name":"Shamsi","orcid":"0000-0001-6981-6938","id":"49D32318-F248-11E8-B48F-1D18A9856A87","last_name":"Emtenani","full_name":"Emtenani, Shamsi"},{"full_name":"Martin, Elliot T","last_name":"Martin","first_name":"Elliot T"},{"full_name":"György, Attila","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87","last_name":"György","orcid":"0000-0002-1819-198X","first_name":"Attila"},{"last_name":"Bicher","id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","full_name":"Bicher, Julia","first_name":"Julia"},{"full_name":"Genger, Jakob-Wendelin","last_name":"Genger","first_name":"Jakob-Wendelin"},{"first_name":"Thomas","full_name":"Köcher, Thomas","last_name":"Köcher"},{"full_name":"Akhmanova, Maria","id":"3425EC26-F248-11E8-B48F-1D18A9856A87","last_name":"Akhmanova","orcid":"0000-0003-1522-3162","first_name":"Maria"},{"first_name":"Mariana","orcid":"0000-0001-8238-480X","last_name":"Pereira Guarda","id":"6de81d9d-e2f2-11eb-945a-af8bc2a60b26","full_name":"Pereira Guarda, Mariana"},{"id":"3047D808-F248-11E8-B48F-1D18A9856A87","last_name":"Roblek","full_name":"Roblek, Marko","first_name":"Marko","orcid":"0000-0001-9588-1389"},{"full_name":"Bergthaler, Andreas","last_name":"Bergthaler","first_name":"Andreas"},{"first_name":"Thomas R","last_name":"Hurd","full_name":"Hurd, Thomas R"},{"last_name":"Rangan","full_name":"Rangan, Prashanth","first_name":"Prashanth"},{"orcid":"0000-0001-8323-8353","first_name":"Daria E","full_name":"Siekhaus, Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus"}],"doi":"10.15252/embj.2021109049","has_accepted_license":"1","_id":"10918","scopus_import":"1","acknowledgement":"We thank the DGRC (NIH grant 2P40OD010949-10A1) for plasmids, the BDSC (NIH grant P40OD018537) and the VDRC for fly stocks, FlyBase for essential genomic information, the BDGP in situ database for data (Tomancak et al, 2007), the IST Austria Bioimaging facility for support, the VBC Core Facilities for RNA sequencing and analysis, and C. Guet, C. Navarro, C. Desplan, T. Lecuit, I. Miguel-Aliaga, and Siekhaus group members for comments on the manuscript. The VBCF Metabolomics Facility is funded by the City of Vienna through the Vienna Business Agency. This work was supported by the Marie Curie CIG 334077/IRTIM (DES), Austrian Science Fund (FWF) Lise Meitner Fellowship M2379-B28 (MA and DES), Austrian Science Fund (FWF) grant ASI_FWF01_P29638S (DES), NIH/NIGMS (R01GM111779-06 (PR), RO1GM135628-01 (PR), European Research Council (ERC) grant no. 677006 “CMIL” (AB), and Natural Sciences and Engineering Research Council of Canada\r\n(RGPIN-2019-06766) (TRH). ","language":[{"iso":"eng"}],"file_date_updated":"2022-03-24T13:22:41Z","corr_author":"1","year":"2022","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"e109049","isi":1,"month":"03","external_id":{"isi":["000771957000001"],"pmid":["35319107"]},"type":"journal_article","quality_controlled":"1","publication_identifier":{"eissn":["1460-2075"]},"intvolume":" 41","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Embo Press","volume":41,"oa":1,"article_type":"original","project":[{"name":"Investigating the role of transporters in invasive migration through junctions","_id":"2536F660-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"334077"},{"name":"Modeling epithelial tissue mechanics during cell invasion","call_identifier":"FWF","grant_number":"M02379","_id":"264CBBAC-B435-11E9-9278-68D0E5697425"},{"name":"The role of Drosophila TNF alpha in immune cell invasion","call_identifier":"FWF","_id":"253B6E48-B435-11E9-9278-68D0E5697425","grant_number":"P29638"}],"pmid":1,"citation":{"apa":"Emtenani, S., Martin, E. T., György, A., Bicher, J., Genger, J.-W., Köcher, T., … Siekhaus, D. E. (2022). Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal. Embo Press. https://doi.org/10.15252/embj.2021109049","ama":"Emtenani S, Martin ET, György A, et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal. 2022;41. doi:10.15252/embj.2021109049","mla":"Emtenani, Shamsi, et al. “Macrophage Mitochondrial Bioenergetics and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The Embo Journal, vol. 41, e109049, Embo Press, 2022, doi:10.15252/embj.2021109049.","short":"S. Emtenani, E.T. Martin, A. György, J. Bicher, J.-W. Genger, T. Köcher, M. Akhmanova, M. Pereira Guarda, M. Roblek, A. Bergthaler, T.R. Hurd, P. Rangan, D.E. Siekhaus, The Embo Journal 41 (2022).","ieee":"S. Emtenani et al., “Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila,” The Embo Journal, vol. 41. Embo Press, 2022.","chicago":"Emtenani, Shamsi, Elliot T Martin, Attila György, Julia Bicher, Jakob-Wendelin Genger, Thomas Köcher, Maria Akhmanova, et al. “Macrophage Mitochondrial Bioenergetics and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The Embo Journal. Embo Press, 2022. https://doi.org/10.15252/embj.2021109049.","ista":"Emtenani S, Martin ET, György A, Bicher J, Genger J-W, Köcher T, Akhmanova M, Pereira Guarda M, Roblek M, Bergthaler A, Hurd TR, Rangan P, Siekhaus DE. 2022. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal. 41, e109049."},"file":[{"date_created":"2022-03-24T13:22:41Z","content_type":"application/pdf","creator":"siekhaus","file_id":"10919","checksum":"dba48580fe0fefaa4c63078d1d2a35df","file_name":"Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosopila.pdf","file_size":4344585,"access_level":"open_access","date_updated":"2022-03-24T13:22:41Z","relation":"main_file"}],"abstract":[{"text":"Cellular metabolism must adapt to changing demands to enable homeostasis. During immune responses or cancer metastasis, cells leading migration into challenging environments require an energy boost, but what controls this capacity is unclear. Here, we study a previously uncharacterized nuclear protein, Atossa (encoded by CG9005), which supports macrophage invasion into the germband of Drosophila by controlling cellular metabolism. First, nuclear Atossa increases mRNA levels of Porthos, a DEAD-box protein, and of two metabolic enzymes, lysine-α-ketoglutarate reductase (LKR/SDH) and NADPH glyoxylate reductase (GR/HPR), thus enhancing mitochondrial bioenergetics. Then Porthos supports ribosome assembly and thereby raises the translational efficiency of a subset of mRNAs, including those affecting mitochondrial functions, the electron transport chain, and metabolism. Mitochondrial respiration measurements, metabolomics, and live imaging indicate that Atossa and Porthos power up OxPhos and energy production to promote the forging of a path into tissues by leading macrophages. Since many crucial physiological responses require increases in mitochondrial energy output, this previously undescribed genetic program may modulate a wide range of cellular behaviors.","lang":"eng"}],"ec_funded":1,"title":"Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila","ddc":["570"],"article_processing_charge":"Yes (via OA deal)","date_published":"2022-03-23T00:00:00Z","oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"}],"day":"23","date_updated":"2025-06-12T06:20:16Z","status":"public","publication_status":"published","publication":"The Embo Journal"},{"abstract":[{"lang":"eng","text":"Optogenetics has been harnessed to shed new mechanistic light on current and future therapeutic strategies. This has been to date achieved by the regulation of ion flow and electrical signals in neuronal cells and neural circuits that are known to be affected by disease. In contrast, the optogenetic delivery of trophic biochemical signals, which support cell survival and are implicated in degenerative disorders, has never been demonstrated in an animal model of disease. Here, we reengineered the human and Drosophila melanogaster REarranged during Transfection (hRET and dRET) receptors to be activated by light, creating one-component optogenetic tools termed Opto-hRET and Opto-dRET. Upon blue light stimulation, these receptors robustly induced the MAPK/ERK proliferative signaling pathway in cultured cells. In PINK1B9 flies that exhibit loss of PTEN-induced putative kinase 1 (PINK1), a kinase associated with familial Parkinson’s disease (PD), light activation of Opto-dRET suppressed mitochondrial defects, tissue degeneration and behavioral deficits. In human cells with PINK1 loss-of-function, mitochondrial fragmentation was rescued using Opto-dRET via the PI3K/NF-кB pathway. Our results demonstrate that a light-activated receptor can ameliorate disease hallmarks in a genetic model of PD. The optogenetic delivery of trophic signals is cell type-specific and reversible and thus has the potential to inspire novel strategies towards a spatio-temporal regulation of tissue repair."}],"ddc":["570"],"title":"Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease","day":"01","date_updated":"2026-04-02T14:07:10Z","oa_version":"Published Version","article_processing_charge":"No","date_published":"2021-04-01T00:00:00Z","publication_status":"published","status":"public","publication":"PLoS genetics","quality_controlled":"1","type":"journal_article","month":"04","external_id":{"isi":["000640606700001"],"pmid":["33857132"]},"page":"e1009479","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"volume":17,"publisher":"Public Library of Science","intvolume":" 17","publication_identifier":{"eissn":["1553-7404"]},"file":[{"file_size":3072764,"access_level":"open_access","date_updated":"2021-05-04T09:05:27Z","relation":"main_file","success":1,"creator":"kschuh","date_created":"2021-05-04T09:05:27Z","content_type":"application/pdf","file_name":"2021_PLOS_Ingles-Prieto.pdf","checksum":"82a74668f863e8dfb22fdd4f845c92ce","file_id":"9369"}],"pmid":1,"citation":{"ieee":"Á. Inglés Prieto et al., “Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease,” PLoS genetics, vol. 17, no. 4. Public Library of Science, p. e1009479, 2021.","ista":"Inglés Prieto Á, Furthmann N, Crossman SH, Tichy AM, Hoyer N, Petersen M, Zheden V, Bicher J, Gschaider-Reichhart E, György A, Siekhaus DE, Soba P, Winklhofer KF, Janovjak HL. 2021. Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease. PLoS genetics. 17(4), e1009479.","chicago":"Inglés Prieto, Álvaro, Nikolas Furthmann, Samuel H. Crossman, Alexandra Madelaine Tichy, Nina Hoyer, Meike Petersen, Vanessa Zheden, et al. “Optogenetic Delivery of Trophic Signals in a Genetic Model of Parkinson’s Disease.” PLoS Genetics. Public Library of Science, 2021. https://doi.org/10.1371/journal.pgen.1009479.","mla":"Inglés Prieto, Álvaro, et al. “Optogenetic Delivery of Trophic Signals in a Genetic Model of Parkinson’s Disease.” PLoS Genetics, vol. 17, no. 4, Public Library of Science, 2021, p. e1009479, doi:10.1371/journal.pgen.1009479.","short":"Á. Inglés Prieto, N. Furthmann, S.H. Crossman, A.M. Tichy, N. Hoyer, M. Petersen, V. Zheden, J. Bicher, E. Gschaider-Reichhart, A. György, D.E. Siekhaus, P. Soba, K.F. Winklhofer, H.L. Janovjak, PLoS Genetics 17 (2021) e1009479.","ama":"Inglés Prieto Á, Furthmann N, Crossman SH, et al. Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease. PLoS genetics. 2021;17(4):e1009479. doi:10.1371/journal.pgen.1009479","apa":"Inglés Prieto, Á., Furthmann, N., Crossman, S. H., Tichy, A. M., Hoyer, N., Petersen, M., … Janovjak, H. L. (2021). Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease. PLoS Genetics. Public Library of Science. https://doi.org/10.1371/journal.pgen.1009479"},"language":[{"iso":"eng"}],"file_date_updated":"2021-05-04T09:05:27Z","isi":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","year":"2021","author":[{"orcid":"0000-0002-5409-8571","first_name":"Álvaro","full_name":"Inglés Prieto, Álvaro","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","last_name":"Inglés Prieto"},{"first_name":"Nikolas","full_name":"Furthmann, Nikolas","last_name":"Furthmann"},{"first_name":"Samuel H.","last_name":"Crossman","full_name":"Crossman, Samuel H."},{"last_name":"Tichy","full_name":"Tichy, Alexandra Madelaine","first_name":"Alexandra Madelaine"},{"last_name":"Hoyer","full_name":"Hoyer, Nina","first_name":"Nina"},{"first_name":"Meike","last_name":"Petersen","full_name":"Petersen, Meike"},{"orcid":"0000-0002-9438-4783","first_name":"Vanessa","full_name":"Zheden, Vanessa","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Bicher","id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","full_name":"Bicher, Julia","first_name":"Julia"},{"first_name":"Eva","orcid":"0000-0002-7218-7738","id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","last_name":"Gschaider-Reichhart","full_name":"Gschaider-Reichhart, Eva"},{"first_name":"Attila","orcid":"0000-0002-1819-198X","last_name":"György","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87","full_name":"György, Attila"},{"full_name":"Siekhaus, Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus","orcid":"0000-0001-8323-8353","first_name":"Daria E"},{"first_name":"Peter","full_name":"Soba, Peter","last_name":"Soba"},{"full_name":"Winklhofer, Konstanze F.","last_name":"Winklhofer","first_name":"Konstanze F."},{"id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","full_name":"Janovjak, Harald L","first_name":"Harald L","orcid":"0000-0002-8023-9315"}],"department":[{"_id":"EM-Fac"},{"_id":"LoSw"},{"_id":"DaSi"}],"date_created":"2021-05-02T22:01:29Z","_id":"9363","doi":"10.1371/journal.pgen.1009479","has_accepted_license":"1","acknowledgement":"We thank R. Cagan, A. Whitworth and J. Nagpal for fly lines and advice, S. Herlitze for provision of a tissue culture illuminator, and Verian Bader for help with statistical analysis.","issue":"4","scopus_import":"1"},{"language":[{"iso":"eng"}],"file_date_updated":"2021-06-28T14:06:24Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/boost-for-mouse-genetic-analysis/","description":"News on IST Homepage","relation":"press_release"}]},"year":"2021","article_number":"109274","isi":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_created":"2021-06-27T22:01:48Z","department":[{"_id":"SiHi"},{"_id":"LoSw"},{"_id":"PreCl"}],"author":[{"id":"475990FE-F248-11E8-B48F-1D18A9856A87","last_name":"Contreras","full_name":"Contreras, Ximena","first_name":"Ximena"},{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","full_name":"Amberg, Nicole","first_name":"Nicole","orcid":"0000-0002-3183-8207"},{"full_name":"Davaatseren, Amarbayasgalan","last_name":"Davaatseren","id":"70ADC922-B424-11E9-99E3-BA18E6697425","first_name":"Amarbayasgalan"},{"full_name":"Hansen, Andi H","last_name":"Hansen","id":"38853E16-F248-11E8-B48F-1D18A9856A87","first_name":"Andi H"},{"full_name":"Sonntag, Johanna","id":"32FE7D7C-F248-11E8-B48F-1D18A9856A87","last_name":"Sonntag","first_name":"Johanna"},{"full_name":"Andersen, Lill","last_name":"Andersen","first_name":"Lill"},{"full_name":"Bernthaler, Tina","last_name":"Bernthaler","first_name":"Tina"},{"first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher"},{"full_name":"Heger, Anna-Magdalena","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87","last_name":"Heger","first_name":"Anna-Magdalena"},{"first_name":"Randy L.","full_name":"Johnson, Randy L.","last_name":"Johnson"},{"full_name":"Schwarz, Lindsay A.","last_name":"Schwarz","first_name":"Lindsay A."},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"},{"first_name":"Thomas","full_name":"Rülicke, Thomas","last_name":"Rülicke"},{"full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","first_name":"Simon"}],"doi":"10.1016/j.celrep.2021.109274","has_accepted_license":"1","_id":"9603","scopus_import":"1","acknowledgement":"We thank the Bioimaging, Life Science, and Pre-Clinical Facilities at IST Austria; M.P. Postiglione, C. Simbriger, K. Valoskova, C. Schwayer, T. Hussain, M. Pieber, and V. Wimmer for initial experiments, technical support, and/or assistance; R. Shigemoto for sharing iv (Dnah11 mutant) mice; and M. Sixt and all members of the Hippenmeyer lab for discussion. This work was supported by National Institutes of Health grants ( R01-NS050580 to L.L. and F32MH096361 to L.A.S.). L.L. is an investigator of HHMI. N.A. received support from FWF Firnberg-Programm ( T 1031 ). A.H.H. is a recipient of a DOC Fellowship (24812) of the Austrian Academy of Sciences . This work also received support from IST Austria institutional funds , FWF SFB F78 to S.H., the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme ( FP7/2007-2013 ) under REA grant agreement no 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement no. 725780 LinPro ) to S.H.","issue":"12","abstract":[{"text":"Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to <25% of all mouse genes on select chromosomes to date. To overcome this limitation, we generate transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validate their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.","lang":"eng"}],"ec_funded":1,"ddc":["570"],"title":"A genome-wide library of MADM mice for single-cell genetic mosaic analysis","article_processing_charge":"No","date_published":"2021-06-22T00:00:00Z","date_updated":"2026-04-02T14:04:28Z","day":"22","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"oa_version":"Published Version","status":"public","publication_status":"published","publication":"Cell Reports","external_id":{"pmid":["34161767"],"isi":["000664463600016"]},"type":"journal_article","month":"06","quality_controlled":"1","intvolume":" 35","publication_identifier":{"eissn":["2211-1247"]},"oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"publisher":"Cell Press","volume":35,"project":[{"name":"Molecular mechanisms of radial neuronal migration","grant_number":"24812","_id":"2625A13E-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FP7","_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444","name":"Molecular Mechanisms of Cerebral Cortex Development"},{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","call_identifier":"H2020","_id":"260018B0-B435-11E9-9278-68D0E5697425","grant_number":"725780"}],"article_type":"original","file":[{"success":1,"relation":"main_file","date_updated":"2021-06-28T14:06:24Z","access_level":"open_access","file_size":7653149,"file_id":"9613","checksum":"d49520fdcbbb5c2f883bddb67cee5d77","file_name":"2021_CellReports_Contreras.pdf","content_type":"application/pdf","date_created":"2021-06-28T14:06:24Z","creator":"asandaue"}],"pmid":1,"citation":{"ama":"Contreras X, Amberg N, Davaatseren A, et al. A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. 2021;35(12). doi:10.1016/j.celrep.2021.109274","apa":"Contreras, X., Amberg, N., Davaatseren, A., Hansen, A. H., Sonntag, J., Andersen, L., … Hippenmeyer, S. (2021). A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2021.109274","chicago":"Contreras, Ximena, Nicole Amberg, Amarbayasgalan Davaatseren, Andi H Hansen, Johanna Sonntag, Lill Andersen, Tina Bernthaler, et al. “A Genome-Wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis.” Cell Reports. Cell Press, 2021. https://doi.org/10.1016/j.celrep.2021.109274.","ista":"Contreras X, Amberg N, Davaatseren A, Hansen AH, Sonntag J, Andersen L, Bernthaler T, Streicher C, Heger A-M, Johnson RL, Schwarz LA, Luo L, Rülicke T, Hippenmeyer S. 2021. A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. 35(12), 109274.","ieee":"X. Contreras et al., “A genome-wide library of MADM mice for single-cell genetic mosaic analysis,” Cell Reports, vol. 35, no. 12. Cell Press, 2021.","short":"X. Contreras, N. Amberg, A. Davaatseren, A.H. Hansen, J. Sonntag, L. Andersen, T. Bernthaler, C. Streicher, A.-M. Heger, R.L. Johnson, L.A. Schwarz, L. Luo, T. Rülicke, S. Hippenmeyer, Cell Reports 35 (2021).","mla":"Contreras, Ximena, et al. “A Genome-Wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis.” Cell Reports, vol. 35, no. 12, 109274, Cell Press, 2021, doi:10.1016/j.celrep.2021.109274."}},{"language":[{"iso":"eng"}],"file_date_updated":"2020-12-03T11:45:26Z","corr_author":"1","year":"2020","isi":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2020-12-03T11:47:31Z","department":[{"_id":"LoSw"}],"author":[{"last_name":"Salamatina","full_name":"Salamatina, Alina","first_name":"Alina"},{"first_name":"Jerry H","full_name":"Yang, Jerry H","last_name":"Yang"},{"full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton","first_name":"Susan"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B ","first_name":"Jay B "},{"full_name":"Fang, Linjing","last_name":"Fang","first_name":"Linjing"},{"last_name":"Kintner","full_name":"Kintner, Christopher R","first_name":"Christopher R"},{"first_name":"Thomas M","full_name":"Jessell, Thomas M","last_name":"Jessell"},{"full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"}],"doi":"10.1016/j.neuroscience.2020.08.011","has_accepted_license":"1","_id":"8914","scopus_import":"1","acknowledgement":"This work was made possible by the generous support of Project ALS. Imaging and related analyses were facilitated by The Waitt Advanced Biophotonics Center Core at the Salk Institute, supported by grants from NIH-NCI CCSG (P30 014195) and NINDS Neuroscience Center (NS072031). The authors would like to additionally thank Drs. Jane Dodd, Robert Brownstone, and Laskaro Zagoraiou for helpful comments on the manuscript. This manuscript is dedicated to Tom Jessell, an inspirational scientist, friend and mentor.","abstract":[{"text":"Amyotrophic lateral sclerosis (ALS) leads to a loss of specific motor neuron populations in the spinal cord and cortex. Emerging evidence suggests that interneurons may also be affected, but a detailed characterization of interneuron loss and its potential impacts on motor neuron loss and disease progression is lacking. To examine this issue, the fate of V1 inhibitory neurons during ALS was assessed in the ventral spinal cord using the SODG93A mouse model. The V1 population makes up ∼30% of all ventral inhibitory neurons, ∼50% of direct inhibitory synaptic contacts onto motor neuron cell bodies, and is thought to play a key role in modulating motor output, in part through recurrent and reciprocal inhibitory circuits. We find that approximately half of V1 inhibitory neurons are lost in SODG93A mice at late disease stages, but that this loss is delayed relative to the loss of motor neurons and V2a excitatory neurons. We further identify V1 subpopulations based on transcription factor expression that are differentially susceptible to degeneration in SODG93A mice. At an early disease stage, we show that V1 synaptic contacts with motor neuron cell bodies increase, suggesting an upregulation of inhibition before V1 neurons are lost in substantial numbers. These data support a model in which progressive changes in V1 synaptic contacts early in disease, and in select V1 subpopulations at later stages, represent a compensatory upregulation and then deleterious breakdown of specific interneuron circuits within the spinal cord.","lang":"eng"}],"ddc":["570"],"title":"Differential loss of spinal interneurons in a mouse model of ALS","article_processing_charge":"Yes (via OA deal)","date_published":"2020-12-01T00:00:00Z","day":"01","date_updated":"2024-10-09T21:00:14Z","oa_version":"Published Version","status":"public","publication":"Neuroscience","publication_status":"published","external_id":{"pmid":["32858144"],"isi":["000595588700008"]},"type":"journal_article","month":"12","page":"81-95","quality_controlled":"1","intvolume":" 450","publication_identifier":{"issn":["0306-4522"]},"publisher":"Elsevier","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"volume":450,"article_type":"original","file":[{"file_id":"8915","file_name":"2020_Neuroscience_Salamatina.pdf","checksum":"da7413c819e079720669c82451b49294","content_type":"application/pdf","date_created":"2020-12-03T11:45:26Z","creator":"dernst","success":1,"relation":"main_file","file_size":4071247,"access_level":"open_access","date_updated":"2020-12-03T11:45:26Z"}],"citation":{"mla":"Salamatina, Alina, et al. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” Neuroscience, vol. 450, Elsevier, 2020, pp. 81–95, doi:10.1016/j.neuroscience.2020.08.011.","short":"A. Salamatina, J.H. Yang, S. Brenner-Morton, J.B. Bikoff, L. Fang, C.R. Kintner, T.M. Jessell, L.B. Sweeney, Neuroscience 450 (2020) 81–95.","ieee":"A. Salamatina et al., “Differential loss of spinal interneurons in a mouse model of ALS,” Neuroscience, vol. 450. Elsevier, pp. 81–95, 2020.","chicago":"Salamatina, Alina, Jerry H Yang, Susan Brenner-Morton, Jay B Bikoff, Linjing Fang, Christopher R Kintner, Thomas M Jessell, and Lora B. Sweeney. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” Neuroscience. Elsevier, 2020. https://doi.org/10.1016/j.neuroscience.2020.08.011.","ista":"Salamatina A, Yang JH, Brenner-Morton S, Bikoff JB, Fang L, Kintner CR, Jessell TM, Sweeney LB. 2020. Differential loss of spinal interneurons in a mouse model of ALS. Neuroscience. 450, 81–95.","apa":"Salamatina, A., Yang, J. H., Brenner-Morton, S., Bikoff, J. B., Fang, L., Kintner, C. R., … Sweeney, L. B. (2020). Differential loss of spinal interneurons in a mouse model of ALS. Neuroscience. Elsevier. https://doi.org/10.1016/j.neuroscience.2020.08.011","ama":"Salamatina A, Yang JH, Brenner-Morton S, et al. Differential loss of spinal interneurons in a mouse model of ALS. Neuroscience. 2020;450:81-95. doi:10.1016/j.neuroscience.2020.08.011"},"pmid":1},{"abstract":[{"lang":"eng","text":"Medicinal bioinorganic chemistry is a thriving field of drug research for cancer treatment. Transition metal complexes coordinated to essential biological scaffolds represent a highly promising class of compounds for design of novel target-specific therapeutics. We report here the biological evaluation of a novel Isatin-Schiff base derivative and its Cu(II) complex in several tumor cell lines by assessing their effects on cellular metabolism, real-time cell proliferation and induction of apoptosis. Further, the impact of compounds on the p53 protein and expression of its target genes, including MDM2, p21/CDKN1A, and PUMA was evaluated. Results obtained in this study provide further evidence in support of our prior data suggesting the p53-mediated mechanism of action for Isatin-Schiff base derivatives and their complexes and also shed light on potential use of these compounds for stimulation of apoptosis in breast cancer cells via activation of the pro-apoptotic PUMA gene."}],"ddc":["570"],"title":"Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors","day":"13","date_updated":"2025-07-10T11:51:52Z","oa_version":"Published Version","date_published":"2018-11-13T00:00:00Z","article_processing_charge":"Yes","publication":"Cell Death Discovery","status":"public","publication_status":"published","extern":"1","quality_controlled":"1","month":"11","external_id":{"pmid":["30455989 "]},"type":"journal_article","oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Springer Nature","volume":4,"intvolume":" 4","OA_place":"publisher","publication_identifier":{"issn":["2058-7716"]},"article_type":"original","citation":{"apa":"Bulatov, E., Sayarova, R., Mingaleeva, R., Miftakhova, R., Gomzikova, M., Ignatev, I., … Barlev, N. A. (2018). Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors. Cell Death Discovery. Springer Nature. https://doi.org/10.1038/s41420-018-0120-z","ama":"Bulatov E, Sayarova R, Mingaleeva R, et al. Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors. Cell Death Discovery. 2018;4. doi:10.1038/s41420-018-0120-z","short":"E. Bulatov, R. Sayarova, R. Mingaleeva, R. Miftakhova, M. Gomzikova, I. Ignatev, A. Petukhov, P. Davidovich, A. Rizvanov, N.A. Barlev, Cell Death Discovery 4 (2018).","mla":"Bulatov, Emil, et al. “Isatin-Schiff Base-Copper (II) Complex Induces Cell Death in P53-Positive Tumors.” Cell Death Discovery, vol. 4, 103, Springer Nature, 2018, doi:10.1038/s41420-018-0120-z.","ista":"Bulatov E, Sayarova R, Mingaleeva R, Miftakhova R, Gomzikova M, Ignatev I, Petukhov A, Davidovich P, Rizvanov A, Barlev NA. 2018. Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors. Cell Death Discovery. 4, 103.","chicago":"Bulatov, Emil, Regina Sayarova, Rimma Mingaleeva, Regina Miftakhova, Marina Gomzikova, Iurii Ignatev, Alexey Petukhov, Pavel Davidovich, Albert Rizvanov, and Nickolai A. Barlev. “Isatin-Schiff Base-Copper (II) Complex Induces Cell Death in P53-Positive Tumors.” Cell Death Discovery. Springer Nature, 2018. https://doi.org/10.1038/s41420-018-0120-z.","ieee":"E. Bulatov et al., “Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors,” Cell Death Discovery, vol. 4. Springer Nature, 2018."},"pmid":1,"language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41420-018-0120-z"}],"OA_type":"gold","article_number":"103","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2018","author":[{"last_name":"Bulatov","full_name":"Bulatov, Emil","first_name":"Emil"},{"full_name":"Sayarova, Regina","last_name":"Sayarova","first_name":"Regina"},{"last_name":"Mingaleeva","full_name":"Mingaleeva, Rimma","first_name":"Rimma"},{"first_name":"Regina","last_name":"Miftakhova","full_name":"Miftakhova, Regina"},{"first_name":"Marina","full_name":"Gomzikova, Marina","last_name":"Gomzikova"},{"full_name":"Ignatev, Iurii","last_name":"Ignatev","id":"2ac71786-dc7d-11ea-9b2f-c5ad4b9faff6","first_name":"Iurii"},{"full_name":"Petukhov, Alexey","last_name":"Petukhov","first_name":"Alexey"},{"full_name":"Davidovich, Pavel","last_name":"Davidovich","first_name":"Pavel"},{"full_name":"Rizvanov, Albert","last_name":"Rizvanov","first_name":"Albert"},{"full_name":"Barlev, Nickolai A.","last_name":"Barlev","first_name":"Nickolai A."}],"date_created":"2025-04-11T01:31:42Z","department":[{"_id":"GradSch"},{"_id":"LoSw"}],"DOAJ_listed":"1","_id":"19544","has_accepted_license":"1","doi":"10.1038/s41420-018-0120-z"}]