---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21746'
abstract:
- lang: eng
text: As vertebrates transitioned from water to land, locomotion shifted from undulatory
swimming to limb-based movement. How spinal circuits and their cell types evolved
to support this transition remains unclear. We leverage frog metamorphosis, which
recapitulates this transition within a single organism, to define how spinal circuits
generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture
is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons.
As limbs develop and their movement complexifies, spinal circuits expand in neuron
number and subtype diversity. This expansion is most pronounced for V1 inhibitory
neurons, which increase ∼70-fold and diversify into transcriptionally distinct
subtypes. Disrupting transcription factors defining emerging motor and V1 populations
reveals molecular segregation between swim and limb circuits, highlighting the
role of subtype diversity in motor coordination. A multifold increase in inhibitory
neuron diversity thus underlies the tail-to-limb locomotor transition, providing
a framework for spinal circuit adaptation during vertebrate evolution.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de
Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript.
We are also grateful to Tom Jessell and Chris Kintner for their scientific insight
and mentorship during the conception of this project. It would also have not been
possible without the technical support of the Aquatics and Imaging and Optics Facility
support teams (ISTA). We thank Martin Estermann for preparing the initial draft
of the graphical abstract and Niki Barolini for the final version. In addition,
we thank our funding sources for providing the resources to do these experiments:
GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon
Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special
Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.),
Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858
(to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH
grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC)
(to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472
(to Z.H.), and Project A.L.S. (to S.B.-M.).'
article_number: '117227'
article_processing_charge: Yes
article_type: original
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Y
full_name: Ignatyev, Y
last_name: Ignatyev
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matthijs Geert
full_name: Smits, Matthijs Geert
id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
last_name: Smits
- first_name: Marco
full_name: Dalla Vecchia, Marco
id: 02a7a869-ff06-11ed-a87f-86649d6077e5
last_name: Dalla Vecchia
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227
apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M.,
Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2026.117227
chicago: Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington,
Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold
Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell
Reports. Elsevier, 2026. https://doi.org/10.1016/j.celrep.2026.117227.
ieee: D. Vijatovic et al., “Multifold increase in spinal inhibitory cell
types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Elsevier,
2026.
ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits
MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto
MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory
cell types with emergence of limb movement. Cell Reports. 45(4), 117227.
mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types
with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227,
Elsevier, 2026, doi:10.1016/j.celrep.2026.117227.
short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild,
M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton,
M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).
corr_author: '1'
date_created: 2026-04-19T22:07:43Z
date_published: 2026-04-28T00:00:00Z
date_updated: 2026-05-04T12:27:06Z
day: '28'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1016/j.celrep.2026.117227
external_id:
pmid:
- '41964955 '
file:
- access_level: open_access
checksum: 0d26cdb5b8d8dec3a911d8261a65cdef
content_type: application/pdf
creator: dernst
date_created: 2026-05-04T12:20:10Z
date_updated: 2026-05-04T12:20:10Z
file_id: '21795'
file_name: 2026_CellReports_Vijatovic.pdf
file_size: 14925958
relation: main_file
success: 1
file_date_updated: 2026-05-04T12:20:10Z
has_accepted_license: '1'
intvolume: ' 45'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Multifold increase in spinal inhibitory cell types with emergence of limb movement
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
OA_place: repository
OA_type: green
_id: '21291'
abstract:
- lang: eng
text: The complexity and specificity of movement in vertebrates is driven by a rich
diversity of spinal motor and interneuron cell types. During development, eleven
spinal cord progenitor domains generate an equivalent number of cardinal neuron
types. How progenitor domains, individual progenitors, and post-mitotic diversity
relate is still unknown. We performed high-resolution, single-progenitor cell
lineage tracing in the embryonic mouse spinal cord using mosaic analysis with
double markers (MADM). Our quantitative study of lineage progression revealed
that spinal cord progenitors undergo highly variable numbers of proliferative,
neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons
migrate radially over large distances, span the dorsoventral axis, and even cross
the midline, demonstrating striking bilaterality. Molecular and morphometric analysis
indicate high levels of progenitor multipotency, with an individual progenitor
capable of producing several molecularly and morphologically distinct neuron types,
as well as astrocytes. These findings redefine spinal cord development as a process
in which lineage variability — rather than rigid progenitor identity — drives
the generation of cellular diversity.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko,
and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB
consortium for comments on\r\nthe manuscript. We are also grateful for the technical
support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA).
In addition, we thank our funding sources for providing\r\nthe resources to do these
experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.);
Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation
Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and
S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby
an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C.
by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S.
and G.C. were\r\nadditionally supported by institutional funds from the ISTA and
the University of Exeter,\r\nrespectively. "
article_processing_charge: No
author:
- first_name: Sophie A
full_name: Gobeil, Sophie A
id: 2f3e9efb-eb24-11ec-86b2-88efb11d59fa
last_name: Gobeil
- first_name: Francisco
full_name: Da Silveira Neto, Francisco
id: 8cfb7412-10a7-11f1-add1-82b44e6418f2
last_name: Da Silveira Neto
- first_name: Giulia
full_name: Silvestrelli, Giulia
id: 12632ae8-799e-11ef-94a2-e5a3b5ef49e9
last_name: Silvestrelli
- first_name: Matthijs Geert
full_name: Smits, Matthijs Geert
id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
last_name: Smits
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal
cord cell type diversity. bioRxiv. doi:10.64898/2026.02.12.705305
apa: Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher,
C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell
type diversity. bioRxiv. https://doi.org/10.64898/2026.02.12.705305
chicago: Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs
Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B.
Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” BioRxiv,
n.d. https://doi.org/10.64898/2026.02.12.705305.
ieee: S. A. Gobeil et al., “Lineage origin of spinal cord cell type diversity,”
bioRxiv. .
ista: Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung
GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity.
bioRxiv, 10.64898/2026.02.12.705305.
mla: Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.”
BioRxiv, doi:10.64898/2026.02.12.705305.
short: S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher,
G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-02-17T11:36:20Z
date_published: 2026-02-16T00:00:00Z
date_updated: 2026-04-14T08:16:55Z
day: '16'
ddc:
- '570'
department:
- _id: SiHi
- _id: LoSw
doi: 10.64898/2026.02.12.705305
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.64898/2026.02.12.705305
month: '02'
oa: 1
oa_version: Preprint
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: bioRxiv
publication_status: submitted
status: public
title: Lineage origin of spinal cord cell type diversity
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: repository
OA_type: green
_id: '21920'
abstract:
- lang: eng
text: 'Vertebrates display remarkable diversity of sensorimotor behaviors, each
adapted to distinct ecological and survival demands. This diversity raises fundamental
questions about the evolutionary origin of motor control: do conserved spinal
circuits underlie these behaviors, and how have they diverged across species.
Recent studies detail spinal cell-type architecture in mammals but comparable,
high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we
compare spinal cord cell types between fish, frogs, mice and humans, spanning
∼450 million years of evolution. Across species, we define highly conserved programs
of cell type specification that segregate spinal neurons into nearly identical
cardinal classes during development. This contrasts with adult stages, when spinal
cell-type composition selectively diverges for excitatory neuron subpopulations.
Using spatial transcriptomics, we localize this species divergence to the superficial,
dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific
cell types. The most dorsal spinal cord thus emerges as a recently evolved hub
for sensory integration in mammals, a neospinal cord analogous to the neocortex.'
acknowledgement: "We would like to thank the members of the Sweeney Lab for discussion
and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing
processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana
Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon
Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program
(SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian
Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of
Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH
award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of
the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions
of the NIH author are considered Works of the United States\r\nGovernment. The findings
and conclusions presented in this paper are those of\r\nthe authors and do not necessarily
reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. "
article_processing_charge: No
author:
- first_name: Yuri
full_name: Ignatyev, Yuri
last_name: Ignatyev
- first_name: Stavros
full_name: Papadopoulos, Stavros
id: 40606b92-f128-11eb-9611-bf66a98cfa5c
last_name: Papadopoulos
- first_name: Mateja
full_name: Soretić, Mateja
last_name: Soretić
- first_name: Jake
full_name: Yeung, Jake
id: 123012b2-db30-11eb-b4d8-a35840c0551b
last_name: Yeung
orcid: 0000-0003-1732-1559
- first_name: Tzi-Yang
full_name: Lin, Tzi-Yang
last_name: Lin
- first_name: Elly M
full_name: Tanaka, Elly M
last_name: Tanaka
- first_name: Leonid
full_name: Peshkin, Leonid
last_name: Peshkin
- first_name: Ariel J
full_name: Levine, Ariel J
last_name: Levine
- first_name: Mariano I
full_name: Gabitto, Mariano I
last_name: Gabitto
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell
type heterogeneity across vertebrate evolution. bioRxiv. doi:10.1101/2025.10.09.680955
apa: Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka,
E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity
across vertebrate evolution. bioRxiv. https://doi.org/10.1101/2025.10.09.680955
chicago: Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang
Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora
B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate
Evolution.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.09.680955.
ieee: Y. Ignatyev et al., “Innovations in spinal cord cell type heterogeneity
across vertebrate evolution,” bioRxiv. .
ista: Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin
L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity
across vertebrate evolution. bioRxiv, 10.1101/2025.10.09.680955.
mla: Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity
across Vertebrate Evolution.” BioRxiv, doi:10.1101/2025.10.09.680955.
short: Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka,
L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-05-27T06:54:04Z
date_published: 2025-10-11T00:00:00Z
date_updated: 2026-05-27T07:25:41Z
day: '11'
department:
- _id: LoSw
- _id: ScienComp
doi: 10.1101/2025.10.09.680955
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.10.09.680955
month: '10'
oa: 1
oa_version: Preprint
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 907b765e-16d5-11f0-9cad-fef108a945b1
grant_number: '27229'
name: 'A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb
transition of Xenopus metamorphosis'
publication: bioRxiv
publication_status: submitted
status: public
title: Innovations in spinal cord cell type heterogeneity across vertebrate evolution
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19366'
abstract:
- lang: eng
text: Staphylococcus aureus (S. aureus) is one of the most common causative agents
of mammary gland infection and mastitis, but the specific role of S. aureus-derived
extracellular vesicles (SaEVs) in mastitis has been poorly studied to date. Here,
we aimed to investigate the response of bovine monocyte-derived macrophages (boMdM)
to SaEVs of the genotype B (GTB) mastitis-related strain M5512B. Specifically,
we evaluated the effects on the actin cytoskeleton, gene expression, and the SaEV
proteomic cargo. Furthermore, we assessed to what extent the cellular and molecular
response of boMdM to SaEVs differed from peripheral mononuclear blood cells (PBMCs)
used for in vitro derivation of the former. We observed that SaEVs induced morphological
changes in boMdM, leading to a pro-inflammatory and pyroptosis-related increased
gene expression. Additionally, our study revealed that boMdM and PBMCs exhibited
stimulus-specific differing responses. The proteomic analysis of SaEVs identified
clusters of proteins related to virulence and antibiotic resistance, supporting
the theory that S. aureus might use EVs to evade host defences and colonize the
mammary gland. Our results bring new insights into how SaEVs might impact the
host during an S. aureus infection, which can be useful for future S. aureus vaccine
development.
acknowledgement: "The authors thank Michele Guastalla for his contributions to the
boMdM analyses and Stephan Handschin from the Scientific Center for Optical and
Electron Microscopy (ScopeM) of ETH Zurich for the TEM imaging. We gratefully acknowledge
the Functional Genomics Center Zurich (FGCZ) for performing the mass spectrometry
analysis for this study.\r\nOpen access funding provided by Swiss Federal Institute
of Technology Zurich. This work was supported by basic funding from ETH Zurich."
article_processing_charge: Yes
article_type: original
author:
- first_name: Mara D.
full_name: Saenz-De-Juano, Mara D.
last_name: Saenz-De-Juano
- first_name: Giulia
full_name: Silvestrelli, Giulia
id: 12632ae8-799e-11ef-94a2-e5a3b5ef49e9
last_name: Silvestrelli
- first_name: Samuel
full_name: Buri, Samuel
last_name: Buri
- first_name: Léa V.
full_name: Zinsli, Léa V.
last_name: Zinsli
- first_name: Mathias
full_name: Schmelcher, Mathias
last_name: Schmelcher
- first_name: Susanne E.
full_name: Ulbrich, Susanne E.
last_name: Ulbrich
citation:
ama: Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich
SE. Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce
a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific
Reports. 2025;15:6059. doi:10.1038/s41598-025-90466-6
apa: Saenz-De-Juano, M. D., Silvestrelli, G., Buri, S., Zinsli, L. V., Schmelcher,
M., & Ulbrich, S. E. (2025). Mastitis-related Staphylococcus aureus-derived
extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived
macrophages. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-025-90466-6
chicago: Saenz-De-Juano, Mara D., Giulia Silvestrelli, Samuel Buri, Léa V. Zinsli,
Mathias Schmelcher, and Susanne E. Ulbrich. “Mastitis-Related Staphylococcus Aureus-Derived
Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived
Macrophages.” Scientific Reports. Springer Nature, 2025. https://doi.org/10.1038/s41598-025-90466-6.
ieee: M. D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L. V. Zinsli, M. Schmelcher,
and S. E. Ulbrich, “Mastitis-related Staphylococcus aureus-derived extracellular
vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages,”
Scientific Reports, vol. 15. Springer Nature, p. 6059, 2025.
ista: Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich
SE. 2025. Mastitis-related Staphylococcus aureus-derived extracellular vesicles
induce a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific
Reports. 15, 6059.
mla: Saenz-De-Juano, Mara D., et al. “Mastitis-Related Staphylococcus Aureus-Derived
Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived
Macrophages.” Scientific Reports, vol. 15, Springer Nature, 2025, p. 6059,
doi:10.1038/s41598-025-90466-6.
short: M.D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L.V. Zinsli, M. Schmelcher,
S.E. Ulbrich, Scientific Reports 15 (2025) 6059.
date_created: 2025-03-09T23:01:26Z
date_published: 2025-02-19T00:00:00Z
date_updated: 2025-09-30T10:58:59Z
day: '19'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1038/s41598-025-90466-6
external_id:
isi:
- '001426697000031'
pmid:
- '39972051'
file:
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checksum: 51b55ae299de1fa126016a11024b499a
content_type: application/pdf
creator: dernst
date_created: 2025-03-10T12:00:34Z
date_updated: 2025-03-10T12:00:34Z
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page: '6059'
pmid: 1
publication: Scientific Reports
publication_identifier:
eissn:
- 2045-2322
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce
a pro-inflammatory response in bovine monocyte-derived macrophages
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '15016'
abstract:
- lang: eng
text: Amphibians, by virtue of their phylogenetic position, provide invaluable insights
on nervous system evolution, development, and remodeling. The genetic toolkit
for amphibians, however, remains limited. Recombinant adeno-associated viral vectors
(AAVs) are a powerful alternative to transgenesis for labeling and manipulating
neurons. Although successful in mammals, AAVs have never been shown to transduce
amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs
Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and
identified at least two AAV serotypes per species that transduce neurons. In developing
amphibians, AAVs labeled groups of neurons generated at the same time during development.
In the mature brain, AAVrg retrogradely traced long-range projections. Our study
introduces AAVs as a tool for amphibian research, establishes a generalizable
workflow for AAV screening in new species, and expands opportunities for cross-species
comparisons of nervous system development, function, and evolution.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: 'We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi,
Kelley, and Cline Labs for their contributions to this project, discussion, and
support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru
(Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral
Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska
Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination;
our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC,
and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T.,
A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.);
FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting
Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund
(FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award
GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.);
EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Eliza C.B.
full_name: Jaeger, Eliza C.B.
last_name: Jaeger
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: Astrid
full_name: Deryckere, Astrid
last_name: Deryckere
- first_name: Nikol
full_name: Zorin, Nikol
last_name: Zorin
- first_name: Akemi L.
full_name: Nguyen, Akemi L.
last_name: Nguyen
- first_name: Georgiy
full_name: Ivanian, Georgiy
id: eaf2b366-cfd1-11ee-bbdf-c8790f800a05
last_name: Ivanian
- first_name: Jamie
full_name: Woych, Jamie
last_name: Woych
- first_name: Rebecca C
full_name: Arnold, Rebecca C
id: d6cce458-14c9-11ed-a755-c1c8fc6fde6f
last_name: Arnold
- first_name: Alonso
full_name: Ortega Gurrola, Alonso
last_name: Ortega Gurrola
- first_name: Arik
full_name: Shvartsman, Arik
last_name: Shvartsman
- first_name: Francesca
full_name: Barbieri, Francesca
id: a9492887-8972-11ed-ae7b-bfae10998254
last_name: Barbieri
- first_name: Florina-Alexandra
full_name: Toma, Florina-Alexandra
id: 85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5
last_name: Toma
- first_name: Gary J.
full_name: Gorbsky, Gary J.
last_name: Gorbsky
- first_name: Marko E.
full_name: Horb, Marko E.
last_name: Horb
- first_name: Hollis T.
full_name: Cline, Hollis T.
last_name: Cline
- first_name: Timothy F.
full_name: Shay, Timothy F.
last_name: Shay
- first_name: Darcy B.
full_name: Kelley, Darcy B.
last_name: Kelley
- first_name: Ayako
full_name: Yamaguchi, Ayako
last_name: Yamaguchi
- first_name: Mark
full_name: Shein-Idelson, Mark
last_name: Shein-Idelson
- first_name: Maria Antonietta
full_name: Tosches, Maria Antonietta
last_name: Tosches
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to
trace neural development and connectivity across amphibians. Developmental
Cell. 2025;60(5):794-812.e6. doi:10.1016/j.devcel.2024.10.025
apa: Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian,
G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development
and connectivity across amphibians. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.10.025
chicago: Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi
L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools
to Trace Neural Development and Connectivity across Amphibians.” Developmental
Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.025.
ieee: E. C. B. Jaeger et al., “Adeno-associated viral tools to trace neural
development and connectivity across amphibians,” Developmental Cell, vol.
60, no. 5. Elsevier, p. 794–812.e6, 2025.
ista: Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych
J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ,
Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA,
Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and
connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.
mla: Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development
and Connectivity across Amphibians.” Developmental Cell, vol. 60, no. 5,
Elsevier, 2025, p. 794–812.e6, doi:10.1016/j.devcel.2024.10.025.
short: E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian,
J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma,
G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M.
Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.
corr_author: '1'
date_created: 2024-02-20T09:20:32Z
date_published: 2025-03-10T00:00:00Z
date_updated: 2025-09-30T10:00:55Z
day: '10'
ddc:
- '570'
department:
- _id: LoSw
- _id: MaDe
- _id: GaNo
doi: 10.1016/j.devcel.2024.10.025
external_id:
isi:
- '001444798600001'
pmid:
- '39603234'
file:
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isi: 1
issue: '5'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 794-812.e6
pmid: 1
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adeno-associated viral tools to trace neural development and connectivity across
amphibians
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 60
year: '2025'
...
---
OA_place: publisher
_id: '20735'
abstract:
- lang: eng
text: "Left–right alternation is a defining feature of spinal locomotor circuits,
yet the level of neuronal\r\ndetail required to generate and maintain this pattern
remains unclear. This thesis investigates how\r\nmodels spanning multiple levels
of abstraction—from biophysically detailed Hodgkin–Huxley (HH)\r\nneurons to adaptive
integrate–and–fire (I&F) formulations and synfire-chain modules—can account\r\nfor
the generation of fictive swimming in the spinal cord of the Xenopus laevis tadpole.
The guiding\r\nhypothesis is that a small set of neuronal mechanisms is sufficient
to reproduce the essential features\r\nof rhythmic alternation, and that moving
between modeling scales helps distinguish core principles\r\nfrom biological detail.\r\nA
minimal bilateral HH network comprising only four canonical neuron classes—excitatory\r\ndescending
interneurons (dINs), inhibitory commissural interneurons (cINs), ipsilateral inhibitory\r\ninterneurons
(aINs) and motoneurons—served as a biophysical proof of concept. Tuned to reproduce\r\nexperimentally
observed firing modes, the model demonstrated that rebound-prone dIN excitability,\r\ncontralateral
inhibition and modest electrical coupling are sufficient to generate stable alternating\r\nactivity,
even in very small networks. These results motivated the transition to simpler
models\r\ncapable of efficient analysis and scaling.\r\nAdaptive exponential I&F
(AdEx) neurons were calibrated to physiological recordings using\r\nsimulation-based
inference, yielding tonic and phasic/rebound templates that preserved the key\r\ndynamical
signatures of the HH model. Phase-plane analysis clarified the mechanisms underlying\r\nsingle-spike
responses and rebound firing in dINs. At network level, the I&F models robustly\r\nreproduced
left–right alternation, while highlighting constraints on synaptic kinetics and
adaptation\r\nneeded to avoid multi-spike responses.\r\nFinally, a synfire-chain
framework provided a complementary, timing-centric perspective, demonstrating
how precise spike synchrony, synaptic delays and minimal inhibitory coupling can
generate\r\nalternating left–right sequences in a feedforward setting. Together,
these approaches converge on a\r\ncommon conclusion: rebound-prone ipsilateral
excitation combined with precisely timed contralateral inhibition constitutes
a sufficient substrate for alternating spinal rhythms.\r\nBy integrating bottom-up
and top-down modeling strategies, this thesis provides a unified, extensible framework
for studying spinal pattern generation. The results show that essential locomotor\r\ndynamics
can be captured across multiple abstraction levels, offering both mechanistic
insight and\r\npractical tools for future data-driven investigations of spinal
circuit development, robustness and\r\nmodulation."
alternative_title:
- ISTA Master's Thesis
article_processing_charge: No
author:
- first_name: Alexia C
full_name: Wilson, Alexia C
id: 5230e794-15b2-11ec-abd3-e2d5335ebd1d
last_name: Wilson
orcid: 0000-0001-6191-1367
citation:
ama: 'Wilson AC. Modelling the spinal cord of a tadpole : Exploring different ways
to model the spinal cord in the Xenopus frog. 2025. doi:10.15479/AT-ISTA-20735'
apa: 'Wilson, A. C. (2025). Modelling the spinal cord of a tadpole : Exploring
different ways to model the spinal cord in the Xenopus frog. Institute of
Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20735'
chicago: 'Wilson, Alexia C. “Modelling the Spinal Cord of a Tadpole : Exploring
Different Ways to Model the Spinal Cord in the Xenopus Frog.” Institute of Science
and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20735.'
ieee: 'A. C. Wilson, “Modelling the spinal cord of a tadpole : Exploring different
ways to model the spinal cord in the Xenopus frog,” Institute of Science and Technology
Austria, 2025.'
ista: 'Wilson AC. 2025. Modelling the spinal cord of a tadpole : Exploring different
ways to model the spinal cord in the Xenopus frog. Institute of Science and Technology
Austria.'
mla: 'Wilson, Alexia C. Modelling the Spinal Cord of a Tadpole : Exploring Different
Ways to Model the Spinal Cord in the Xenopus Frog. Institute of Science and
Technology Austria, 2025, doi:10.15479/AT-ISTA-20735.'
short: 'A.C. Wilson, Modelling the Spinal Cord of a Tadpole : Exploring Different
Ways to Model the Spinal Cord in the Xenopus Frog, Institute of Science and Technology
Austria, 2025.'
corr_author: '1'
date_created: 2025-12-08T09:49:41Z
date_published: 2025-12-09T00:00:00Z
date_updated: 2026-04-07T12:36:08Z
day: '09'
ddc:
- '570'
- '596'
- '005'
degree_awarded: MS
department:
- _id: GradSch
- _id: TiVo
- _id: LoSw
doi: 10.15479/AT-ISTA-20735
file:
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checksum: 9e3b6b73f8cbec2c3687d17fe8e30410
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date_created: 2026-01-01T17:26:30Z
date_updated: 2026-01-02T13:05:07Z
file_id: '20919'
file_name: tadpoleAdEx.zip
file_size: 566072368
relation: source_file
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checksum: 13f4c0d33923e9d5c9d56731345cf21d
content_type: application/pdf
creator: awilson
date_created: 2026-01-04T12:58:49Z
date_updated: 2026-01-04T12:58:49Z
file_id: '20923'
file_name: Masters_Thesis_Alexia_Wilson_FINAL_pdfA.pdf
file_size: 7170097
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success: 1
file_date_updated: 2026-01-04T12:58:49Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: '110'
publication_identifier:
issn:
- 2791-4585
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '13097'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Tim P
full_name: Vogels, Tim P
id: CB6FF8D2-008F-11EA-8E08-2637E6697425
last_name: Vogels
orcid: 0000-0003-3295-6181
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
title: 'Modelling the spinal cord of a tadpole : Exploring different ways to model
the spinal cord in the Xenopus frog'
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '19520'
abstract:
- lang: eng
text: Vertebrates exhibit a wide range of motor behaviors, ranging from swimming
to complex limb-based movements. Here we take advantage of frog metamorphosis,
which captures a swim-to-limb-based movement transformation during the development
of a single organism, to explore changes in the underlying spinal circuits. We
find that the tadpole spinal cord contains small and largely homogeneous populations
of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages.
These neuronal populations only modestly increase in number and subtype heterogeneity
with the emergence of free swimming. In contrast, during frog metamorphosis and
the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron
number and transcriptional heterogeneity, culminating in cohorts of neurons that
exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated
gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively,
results in severe but selective deficits in tail and limb function. Our work thus
demonstrates that neural diversity scales exponentially with increasing behavioral
complexity and illustrates striking evolutionary conservation in the molecular
organization and function of motor circuits across species.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We would like to thank the members of the Sweeney Lab (especially
Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project
and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion,
and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their
scientific insight and mentorship during the conception of this project. This\r\nproject
would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena
Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA).
In addition, we thank our funding sources for providing the resources to do these\r\nexperiments:
FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon
Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch
Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS
5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.);
NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities
(ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472
(Z.H.); and Project A.L.S. (S.B-M.). "
article_processing_charge: No
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales
exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv.
doi:10.1101/2024.09.20.614050
apa: Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R.,
Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis. bioRxiv. https://doi.org/10.1101/2024.09.20.614050
chicago: Vijatovic, David, Florina Alexandra Toma, Zoe P Harrington, Christoph
M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal
Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during
Frog Metamorphosis.” BioRxiv, n.d. https://doi.org/10.1101/2024.09.20.614050.
ieee: D. Vijatovic et al., “Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis,” bioRxiv. .
ista: Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ,
Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney
LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis. bioRxiv, 10.1101/2024.09.20.614050.
mla: Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with
Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, doi:10.1101/2024.09.20.614050.
short: D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J.
Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen,
J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2025-04-07T08:48:28Z
date_published: 2024-09-27T00:00:00Z
date_updated: 2025-05-14T11:40:13Z
day: '27'
department:
- _id: LoSw
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1101/2024.09.20.614050
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.09.20.614050
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: bioRxiv
publication_status: submitted
status: public
title: Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
OA_embargo: 6 months
OA_place: publisher
OA_type: hybrid
_id: '18305'
abstract:
- lang: eng
text: Motor circuits represent the main output of the central nervous system and
produce dynamic behaviors ranging from relatively simple rhythmic activities like
swimming in fish and breathing in mammals to highly sophisticated dexterous movements
in humans. Despite decades of research, the development and function of motor
circuits remain poorly understood. Breakthroughs in the field recently provided
new tools and tractable model systems that set the stage to discover the molecular
mechanisms and circuit logic underlying motor control. Here, we describe recent
advances from both vertebrate (mouse, frog) and invertebrate (nematode, fruit
fly) systems on cellular and molecular mechanisms that enable motor circuits to
develop and function and highlight conserved and divergent mechanisms necessary
for motor circuit development.
acknowledgement: Work in the authors’ labs is funded by the Helmholtz Association
(N.Z.), National Institute of Neurological Disorders and Stroke (NINDS) R01NS116365
(P.K.), NINDS R01NS123439 and National Science Foundation IOS-2048080 (R.C.), NINDS
R01NS114510 (P.P.), Natural Sciences and Engineering Research Council of Canada
RGPIN-2021-03154 (K.M.) and Horizon Europe European Research Council Starting Grant
Number 101041551 (L.B.S.). P.P. is the Weidenthal Family Designated Professor in
Career Development.
article_number: e1238242024
article_processing_charge: No
article_type: original
author:
- first_name: Paschalis
full_name: Kratsios, Paschalis
last_name: Kratsios
- first_name: Niccolò
full_name: Zampieri, Niccolò
last_name: Zampieri
- first_name: Robert
full_name: Carrillo, Robert
last_name: Carrillo
- first_name: Kota
full_name: Mizumoto, Kota
last_name: Mizumoto
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
- first_name: Polyxeni
full_name: Philippidou, Polyxeni
last_name: Philippidou
citation:
ama: Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P.
Molecular and cellular mechanisms of motor circuit development. The Journal
of Neuroscience. 2024;44(40). doi:10.1523/JNEUROSCI.1238-24.2024
apa: Kratsios, P., Zampieri, N., Carrillo, R., Mizumoto, K., Sweeney, L. B., &
Philippidou, P. (2024). Molecular and cellular mechanisms of motor circuit development.
The Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.1238-24.2024
chicago: Kratsios, Paschalis, Niccolò Zampieri, Robert Carrillo, Kota Mizumoto,
Lora B. Sweeney, and Polyxeni Philippidou. “Molecular and Cellular Mechanisms
of Motor Circuit Development.” The Journal of Neuroscience. Society for
Neuroscience, 2024. https://doi.org/10.1523/JNEUROSCI.1238-24.2024.
ieee: P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L. B. Sweeney, and P.
Philippidou, “Molecular and cellular mechanisms of motor circuit development,”
The Journal of Neuroscience, vol. 44, no. 40. Society for Neuroscience,
2024.
ista: Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P.
2024. Molecular and cellular mechanisms of motor circuit development. The Journal
of Neuroscience. 44(40), e1238242024.
mla: Kratsios, Paschalis, et al. “Molecular and Cellular Mechanisms of Motor Circuit
Development.” The Journal of Neuroscience, vol. 44, no. 40, e1238242024,
Society for Neuroscience, 2024, doi:10.1523/JNEUROSCI.1238-24.2024.
short: P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L.B. Sweeney, P. Philippidou,
The Journal of Neuroscience 44 (2024).
date_created: 2024-10-13T22:01:49Z
date_published: 2024-10-02T00:00:00Z
date_updated: 2026-01-05T14:01:26Z
day: '02'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1523/JNEUROSCI.1238-24.2024
external_id:
isi:
- '001335212200016'
pmid:
- '39358025'
has_accepted_license: '1'
intvolume: ' 44'
isi: 1
issue: '40'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1523/JNEUROSCI.1238-24.2024
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
publication: The Journal of Neuroscience
publication_identifier:
eissn:
- 1529-2401
publication_status: published
publisher: Society for Neuroscience
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular and cellular mechanisms of motor circuit development
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2024'
...
---
_id: '15335'
abstract:
- lang: eng
text: Stable matching of neurotransmitters with their receptors is fundamental to
synapse function and reliable communication in neural circuits. Presynaptic neurotransmitters
regulate the stabilization of postsynaptic transmitter receptors. Whether postsynaptic
receptors regulate stabilization of presynaptic transmitters has received less
attention. Here, we show that blockade of endogenous postsynaptic acetylcholine
receptors (AChR) at the neuromuscular junction destabilizes the cholinergic phenotype
in motor neurons and stabilizes an earlier, developmentally transient glutamatergic
phenotype. Further, expression of exogenous postsynaptic gamma-aminobutyric acid
type A receptors (GABAA receptors) in muscle cells stabilizes an earlier, developmentally
transient GABAergic motor neuron phenotype. Both AChR and GABAA receptors are
linked to presynaptic neurons through transsynaptic bridges. Knockdown of specific
components of these transsynaptic bridges prevents stabilization of the cholinergic
or GABAergic phenotypes. Bidirectional communication can enforce a match between
transmitter and receptor and ensure the fidelity of synaptic transmission. Our
findings suggest a potential role of dysfunctional transmitter receptors in neurological
disorders that involve the loss of the presynaptic transmitter.
acknowledgement: We thank all members of the Spitzer laboratory for discussions and critical feedback; K. Marek for discussions of acknowledgment signals;
I. Gregor and R. Aricescu for discussions of receptor pharmacology and transsynaptic bridges; C. Kintner for advice on Xenopus blastomere lineage; A. Ray
and E. Park for guidance on miniature analysis; A. Glavis- Bloom, S.U. Choi, S.
Atkins, M. Gupta, and S. Malladi for technical assistance; and D. K. Berg and L.
R. Squire for comments on the manuscript. This work was supported by NSF 2051555
and the Overland Foundation. Microscopy for five- channel imaging utilized the UCSD
School of Medicine Microscopy Core, supported by NIH grant NS047101.
article_number: e2318041121
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Swetha K.
full_name: Godavarthi, Swetha K.
last_name: Godavarthi
- first_name: Masaki
full_name: Hiramoto, Masaki
last_name: Hiramoto
- first_name: Yuri
full_name: Ignatyev, Yuri
last_name: Ignatyev
- first_name: Jacqueline B.
full_name: Levin, Jacqueline B.
last_name: Levin
- first_name: Hui Quan
full_name: Li, Hui Quan
last_name: Li
- first_name: Marta
full_name: Pratelli, Marta
last_name: Pratelli
- first_name: Jennifer
full_name: Borchardt, Jennifer
last_name: Borchardt
- first_name: Cynthia
full_name: Czajkowski, Cynthia
last_name: Czajkowski
- first_name: Laura N.
full_name: Borodinsky, Laura N.
last_name: Borodinsky
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
- first_name: Hollis T.
full_name: Cline, Hollis T.
last_name: Cline
- first_name: Nicholas C.
full_name: Spitzer, Nicholas C.
last_name: Spitzer
citation:
ama: Godavarthi SK, Hiramoto M, Ignatyev Y, et al. Postsynaptic receptors regulate
presynaptic transmitter stability through transsynaptic bridges. Proceedings
of the National Academy of Sciences of the United States of America. 2024;121(15).
doi:10.1073/pnas.2318041121
apa: Godavarthi, S. K., Hiramoto, M., Ignatyev, Y., Levin, J. B., Li, H. Q., Pratelli,
M., … Spitzer, N. C. (2024). Postsynaptic receptors regulate presynaptic transmitter
stability through transsynaptic bridges. Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences.
https://doi.org/10.1073/pnas.2318041121
chicago: Godavarthi, Swetha K., Masaki Hiramoto, Yuri Ignatyev, Jacqueline B. Levin,
Hui Quan Li, Marta Pratelli, Jennifer Borchardt, et al. “Postsynaptic Receptors
Regulate Presynaptic Transmitter Stability through Transsynaptic Bridges.” Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2024. https://doi.org/10.1073/pnas.2318041121.
ieee: S. K. Godavarthi et al., “Postsynaptic receptors regulate presynaptic
transmitter stability through transsynaptic bridges,” Proceedings of the National
Academy of Sciences of the United States of America, vol. 121, no. 15. National
Academy of Sciences, 2024.
ista: Godavarthi SK, Hiramoto M, Ignatyev Y, Levin JB, Li HQ, Pratelli M, Borchardt
J, Czajkowski C, Borodinsky LN, Sweeney LB, Cline HT, Spitzer NC. 2024. Postsynaptic
receptors regulate presynaptic transmitter stability through transsynaptic bridges.
Proceedings of the National Academy of Sciences of the United States of America.
121(15), e2318041121.
mla: Godavarthi, Swetha K., et al. “Postsynaptic Receptors Regulate Presynaptic
Transmitter Stability through Transsynaptic Bridges.” Proceedings of the National
Academy of Sciences of the United States of America, vol. 121, no. 15, e2318041121,
National Academy of Sciences, 2024, doi:10.1073/pnas.2318041121.
short: S.K. Godavarthi, M. Hiramoto, Y. Ignatyev, J.B. Levin, H.Q. Li, M. Pratelli,
J. Borchardt, C. Czajkowski, L.N. Borodinsky, L.B. Sweeney, H.T. Cline, N.C. Spitzer,
Proceedings of the National Academy of Sciences of the United States of America
121 (2024).
date_created: 2024-04-21T22:00:53Z
date_published: 2024-04-09T00:00:00Z
date_updated: 2025-09-04T13:42:01Z
day: '09'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1073/pnas.2318041121
external_id:
isi:
- '001243892800004'
pmid:
- '38568976'
file:
- access_level: open_access
checksum: f3b4ffad4ef3d1c443414edf0cd2392c
content_type: application/pdf
creator: dernst
date_created: 2024-04-23T06:53:14Z
date_updated: 2024-04-23T06:53:14Z
file_id: '15340'
file_name: 2024_PNAS_Godavarthi.pdf
file_size: 16187094
relation: main_file
success: 1
file_date_updated: 2024-04-23T06:53:14Z
has_accepted_license: '1'
intvolume: ' 121'
isi: 1
issue: '15'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic
bridges
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 121
year: '2024'
...
---
_id: '13097'
abstract:
- lang: eng
text: 'Vertebrate movement is orchestrated by spinal inter- and motor neurons that,
together with sensory and cognitive input, produce dynamic motor behaviors. These
behaviors vary from the simple undulatory swimming of fish and larval aquatic
species to the highly coordinated running, reaching and grasping of mice, humans
and other mammals. This variation raises the fundamental question of how spinal
circuits have changed in register with motor behavior. In simple, undulatory fish,
exemplified by the lamprey, two broad classes of interneurons shape motor neuron
output: ipsilateral-projecting excitatory neurons, and commissural-projecting
inhibitory neurons. An additional class of ipsilateral inhibitory neurons is required
to generate escape swim behavior in larval zebrafish and tadpoles. In limbed vertebrates,
a more complex spinal neuron composition is observed. In this review, we provide
evidence that movement elaboration correlates with an increase and specialization
of these three basic interneuron types into molecularly, anatomically, and functionally
distinct subpopulations. We summarize recent work linking neuron types to movement-pattern
generation across fish, amphibians, reptiles, birds and mammals.'
acknowledgement: 'This work was supported by the ERC Starting grant, ERC-2021-STG
#101041551.'
article_number: '1146449'
article_processing_charge: Yes
article_type: original
author:
- first_name: Alexia C
full_name: Wilson, Alexia C
id: 5230e794-15b2-11ec-abd3-e2d5335ebd1d
last_name: Wilson
orcid: 0000-0001-6191-1367
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: 'Wilson AC, Sweeney LB. Spinal cords: Symphonies of interneurons across species.
Frontiers in Neural Circuits. 2023;17. doi:10.3389/fncir.2023.1146449'
apa: 'Wilson, A. C., & Sweeney, L. B. (2023). Spinal cords: Symphonies of interneurons
across species. Frontiers in Neural Circuits. Frontiers. https://doi.org/10.3389/fncir.2023.1146449'
chicago: 'Wilson, Alexia C, and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons
across Species.” Frontiers in Neural Circuits. Frontiers, 2023. https://doi.org/10.3389/fncir.2023.1146449.'
ieee: 'A. C. Wilson and L. B. Sweeney, “Spinal cords: Symphonies of interneurons
across species,” Frontiers in Neural Circuits, vol. 17. Frontiers, 2023.'
ista: 'Wilson AC, Sweeney LB. 2023. Spinal cords: Symphonies of interneurons across
species. Frontiers in Neural Circuits. 17, 1146449.'
mla: 'Wilson, Alexia C., and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons
across Species.” Frontiers in Neural Circuits, vol. 17, 1146449, Frontiers,
2023, doi:10.3389/fncir.2023.1146449.'
short: A.C. Wilson, L.B. Sweeney, Frontiers in Neural Circuits 17 (2023).
corr_author: '1'
date_created: 2023-05-28T22:01:04Z
date_published: 2023-04-26T00:00:00Z
date_updated: 2026-04-07T12:36:07Z
day: '26'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.3389/fncir.2023.1146449
external_id:
isi:
- '000984606200001'
pmid:
- '37180760'
file:
- access_level: open_access
checksum: 7efd06de284a28e91e97127611a9c3fd
content_type: application/pdf
creator: dernst
date_created: 2024-01-03T13:33:21Z
date_updated: 2024-01-03T13:33:21Z
file_id: '14729'
file_name: 2023_FrontiersNeuralCircuits_Wilson.pdf
file_size: 6667157
relation: main_file
success: 1
file_date_updated: 2024-01-03T13:33:21Z
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
publication: Frontiers in Neural Circuits
publication_identifier:
issn:
- 1662-5110
publication_status: published
publisher: Frontiers
quality_controlled: '1'
related_material:
record:
- id: '20735'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Spinal cords: Symphonies of interneurons across species'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2023'
...
---
_id: '10918'
abstract:
- lang: eng
text: Cellular metabolism must adapt to changing demands to enable homeostasis.
During immune responses or cancer metastasis, cells leading migration into challenging
environments require an energy boost, but what controls this capacity is unclear.
Here, we study a previously uncharacterized nuclear protein, Atossa (encoded by
CG9005), which supports macrophage invasion into the germband of Drosophila by
controlling cellular metabolism. First, nuclear Atossa increases mRNA levels of
Porthos, a DEAD-box protein, and of two metabolic enzymes, lysine-α-ketoglutarate
reductase (LKR/SDH) and NADPH glyoxylate reductase (GR/HPR), thus enhancing mitochondrial
bioenergetics. Then Porthos supports ribosome assembly and thereby raises the
translational efficiency of a subset of mRNAs, including those affecting mitochondrial
functions, the electron transport chain, and metabolism. Mitochondrial respiration
measurements, metabolomics, and live imaging indicate that Atossa and Porthos
power up OxPhos and energy production to promote the forging of a path into tissues
by leading macrophages. Since many crucial physiological responses require increases
in mitochondrial energy output, this previously undescribed genetic program may
modulate a wide range of cellular behaviors.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We thank the DGRC (NIH grant 2P40OD010949-10A1) for plasmids, the
BDSC (NIH grant P40OD018537) and the VDRC for fly stocks, FlyBase for essential
genomic information, the BDGP in situ database for data (Tomancak et al, 2007),
the IST Austria Bioimaging facility for support, the VBC Core Facilities for RNA
sequencing and analysis, and C. Guet, C. Navarro, C. Desplan, T. Lecuit, I. Miguel-Aliaga,
and Siekhaus group members for comments on the manuscript. The VBCF Metabolomics
Facility is funded by the City of Vienna through the Vienna Business Agency. This
work was supported by the Marie Curie CIG 334077/IRTIM (DES), Austrian Science Fund
(FWF) Lise Meitner Fellowship M2379-B28 (MA and DES), Austrian Science Fund (FWF)
grant ASI_FWF01_P29638S (DES), NIH/NIGMS (R01GM111779-06 (PR), RO1GM135628-01 (PR),
European Research Council (ERC) grant no. 677006 “CMIL” (AB), and Natural Sciences
and Engineering Research Council of Canada\r\n(RGPIN-2019-06766) (TRH). "
article_number: e109049
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Elliot T
full_name: Martin, Elliot T
last_name: Martin
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Julia
full_name: Bicher, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Bicher
- first_name: Jakob-Wendelin
full_name: Genger, Jakob-Wendelin
last_name: Genger
- first_name: Thomas
full_name: Köcher, Thomas
last_name: Köcher
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Mariana
full_name: Pereira Guarda, Mariana
id: 6de81d9d-e2f2-11eb-945a-af8bc2a60b26
last_name: Pereira Guarda
orcid: 0000-0001-8238-480X
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Andreas
full_name: Bergthaler, Andreas
last_name: Bergthaler
- first_name: Thomas R
full_name: Hurd, Thomas R
last_name: Hurd
- first_name: Prashanth
full_name: Rangan, Prashanth
last_name: Rangan
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Emtenani S, Martin ET, György A, et al. Macrophage mitochondrial bioenergetics
and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The
Embo Journal. 2022;41. doi:10.15252/embj.2021109049
apa: Emtenani, S., Martin, E. T., György, A., Bicher, J., Genger, J.-W., Köcher,
T., … Siekhaus, D. E. (2022). Macrophage mitochondrial bioenergetics and tissue
invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal.
Embo Press. https://doi.org/10.15252/embj.2021109049
chicago: Emtenani, Shamsi, Elliot T Martin, Attila György, Julia Bicher, Jakob-Wendelin
Genger, Thomas Köcher, Maria Akhmanova, et al. “Macrophage Mitochondrial Bioenergetics
and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The
Embo Journal. Embo Press, 2022. https://doi.org/10.15252/embj.2021109049.
ieee: S. Emtenani et al., “Macrophage mitochondrial bioenergetics and tissue
invasion are boosted by an Atossa-Porthos axis in Drosophila,” The Embo Journal,
vol. 41. Embo Press, 2022.
ista: Emtenani S, Martin ET, György A, Bicher J, Genger J-W, Köcher T, Akhmanova
M, Pereira Guarda M, Roblek M, Bergthaler A, Hurd TR, Rangan P, Siekhaus DE. 2022.
Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos
axis in Drosophila. The Embo Journal. 41, e109049.
mla: Emtenani, Shamsi, et al. “Macrophage Mitochondrial Bioenergetics and Tissue
Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The Embo Journal,
vol. 41, e109049, Embo Press, 2022, doi:10.15252/embj.2021109049.
short: S. Emtenani, E.T. Martin, A. György, J. Bicher, J.-W. Genger, T. Köcher,
M. Akhmanova, M. Pereira Guarda, M. Roblek, A. Bergthaler, T.R. Hurd, P. Rangan,
D.E. Siekhaus, The Embo Journal 41 (2022).
corr_author: '1'
date_created: 2022-03-24T13:23:09Z
date_published: 2022-03-23T00:00:00Z
date_updated: 2025-06-12T06:20:16Z
day: '23'
ddc:
- '570'
department:
- _id: DaSi
- _id: LoSw
doi: 10.15252/embj.2021109049
ec_funded: 1
external_id:
isi:
- '000771957000001'
pmid:
- '35319107'
file:
- access_level: open_access
checksum: dba48580fe0fefaa4c63078d1d2a35df
content_type: application/pdf
creator: siekhaus
date_created: 2022-03-24T13:22:41Z
date_updated: 2022-03-24T13:22:41Z
file_id: '10919'
file_name: Macrophage mitochondrial bioenergetics and tissue invasion are boosted
by an Atossa-Porthos axis in Drosopila.pdf
file_size: 4344585
relation: main_file
file_date_updated: 2022-03-24T13:22:41Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 264CBBAC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02379
name: Modeling epithelial tissue mechanics during cell invasion
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: The role of Drosophila TNF alpha in immune cell invasion
publication: The Embo Journal
publication_identifier:
eissn:
- 1460-2075
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an
Atossa-Porthos axis in Drosophila
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 41
year: '2022'
...
---
_id: '9363'
abstract:
- lang: eng
text: Optogenetics has been harnessed to shed new mechanistic light on current and
future therapeutic strategies. This has been to date achieved by the regulation
of ion flow and electrical signals in neuronal cells and neural circuits that
are known to be affected by disease. In contrast, the optogenetic delivery of
trophic biochemical signals, which support cell survival and are implicated in
degenerative disorders, has never been demonstrated in an animal model of disease.
Here, we reengineered the human and Drosophila melanogaster REarranged during
Transfection (hRET and dRET) receptors to be activated by light, creating one-component
optogenetic tools termed Opto-hRET and Opto-dRET. Upon blue light stimulation,
these receptors robustly induced the MAPK/ERK proliferative signaling pathway
in cultured cells. In PINK1B9 flies that exhibit loss of PTEN-induced putative
kinase 1 (PINK1), a kinase associated with familial Parkinson’s disease (PD),
light activation of Opto-dRET suppressed mitochondrial defects, tissue degeneration
and behavioral deficits. In human cells with PINK1 loss-of-function, mitochondrial
fragmentation was rescued using Opto-dRET via the PI3K/NF-кB pathway. Our results
demonstrate that a light-activated receptor can ameliorate disease hallmarks in
a genetic model of PD. The optogenetic delivery of trophic signals is cell type-specific
and reversible and thus has the potential to inspire novel strategies towards
a spatio-temporal regulation of tissue repair.
acknowledgement: We thank R. Cagan, A. Whitworth and J. Nagpal for fly lines and advice,
S. Herlitze for provision of a tissue culture illuminator, and Verian Bader for
help with statistical analysis.
article_processing_charge: No
author:
- first_name: Álvaro
full_name: Inglés Prieto, Álvaro
id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
last_name: Inglés Prieto
orcid: 0000-0002-5409-8571
- first_name: Nikolas
full_name: Furthmann, Nikolas
last_name: Furthmann
- first_name: Samuel H.
full_name: Crossman, Samuel H.
last_name: Crossman
- first_name: Alexandra Madelaine
full_name: Tichy, Alexandra Madelaine
last_name: Tichy
- first_name: Nina
full_name: Hoyer, Nina
last_name: Hoyer
- first_name: Meike
full_name: Petersen, Meike
last_name: Petersen
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Julia
full_name: Bicher, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Bicher
- first_name: Eva
full_name: Gschaider-Reichhart, Eva
id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
last_name: Gschaider-Reichhart
orcid: 0000-0002-7218-7738
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Peter
full_name: Soba, Peter
last_name: Soba
- first_name: Konstanze F.
full_name: Winklhofer, Konstanze F.
last_name: Winklhofer
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
citation:
ama: Inglés Prieto Á, Furthmann N, Crossman SH, et al. Optogenetic delivery of trophic
signals in a genetic model of Parkinson’s disease. PLoS genetics. 2021;17(4):e1009479.
doi:10.1371/journal.pgen.1009479
apa: Inglés Prieto, Á., Furthmann, N., Crossman, S. H., Tichy, A. M., Hoyer, N.,
Petersen, M., … Janovjak, H. L. (2021). Optogenetic delivery of trophic signals
in a genetic model of Parkinson’s disease. PLoS Genetics. Public Library
of Science. https://doi.org/10.1371/journal.pgen.1009479
chicago: Inglés Prieto, Álvaro, Nikolas Furthmann, Samuel H. Crossman, Alexandra
Madelaine Tichy, Nina Hoyer, Meike Petersen, Vanessa Zheden, et al. “Optogenetic
Delivery of Trophic Signals in a Genetic Model of Parkinson’s Disease.” PLoS
Genetics. Public Library of Science, 2021. https://doi.org/10.1371/journal.pgen.1009479.
ieee: Á. Inglés Prieto et al., “Optogenetic delivery of trophic signals in
a genetic model of Parkinson’s disease,” PLoS genetics, vol. 17, no. 4.
Public Library of Science, p. e1009479, 2021.
ista: Inglés Prieto Á, Furthmann N, Crossman SH, Tichy AM, Hoyer N, Petersen M,
Zheden V, Bicher J, Gschaider-Reichhart E, György A, Siekhaus DE, Soba P, Winklhofer
KF, Janovjak HL. 2021. Optogenetic delivery of trophic signals in a genetic model
of Parkinson’s disease. PLoS genetics. 17(4), e1009479.
mla: Inglés Prieto, Álvaro, et al. “Optogenetic Delivery of Trophic Signals in a
Genetic Model of Parkinson’s Disease.” PLoS Genetics, vol. 17, no. 4, Public
Library of Science, 2021, p. e1009479, doi:10.1371/journal.pgen.1009479.
short: Á. Inglés Prieto, N. Furthmann, S.H. Crossman, A.M. Tichy, N. Hoyer, M. Petersen,
V. Zheden, J. Bicher, E. Gschaider-Reichhart, A. György, D.E. Siekhaus, P. Soba,
K.F. Winklhofer, H.L. Janovjak, PLoS Genetics 17 (2021) e1009479.
date_created: 2021-05-02T22:01:29Z
date_published: 2021-04-01T00:00:00Z
date_updated: 2026-04-02T14:07:10Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: LoSw
- _id: DaSi
doi: 10.1371/journal.pgen.1009479
external_id:
isi:
- '000640606700001'
pmid:
- '33857132'
file:
- access_level: open_access
checksum: 82a74668f863e8dfb22fdd4f845c92ce
content_type: application/pdf
creator: kschuh
date_created: 2021-05-04T09:05:27Z
date_updated: 2021-05-04T09:05:27Z
file_id: '9369'
file_name: 2021_PLOS_Ingles-Prieto.pdf
file_size: 3072764
relation: main_file
success: 1
file_date_updated: 2021-05-04T09:05:27Z
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: e1009479
pmid: 1
publication: PLoS genetics
publication_identifier:
eissn:
- 1553-7404
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 17
year: '2021'
...
---
_id: '9603'
abstract:
- lang: eng
text: Mosaic analysis with double markers (MADM) offers one approach to visualize
and concomitantly manipulate genetically defined cells in mice with single-cell
resolution. MADM applications include the analysis of lineage, single-cell morphology
and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous
gene functions in vivo in health and disease. Yet, MADM can only be applied to
<25% of all mouse genes on select chromosomes to date. To overcome this limitation,
we generate transgenic mice with knocked-in MADM cassettes near the centromeres
of all 19 autosomes and validate their use across organs. With this resource,
>96% of the entire mouse genome can now be subjected to single-cell genetic mosaic
analysis. Beyond a proof of principle, we apply our MADM library to systematically
trace sister chromatid segregation in distinct mitotic cell lineages. We find
striking chromosome-specific biases in segregation patterns, reflecting a putative
mechanism for the asymmetric segregation of genetic determinants in somatic stem
cell division.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: We thank the Bioimaging, Life Science, and Pre-Clinical Facilities
at IST Austria; M.P. Postiglione, C. Simbriger, K. Valoskova, C. Schwayer, T. Hussain,
M. Pieber, and V. Wimmer for initial experiments, technical support, and/or assistance;
R. Shigemoto for sharing iv (Dnah11 mutant) mice; and M. Sixt and all members of
the Hippenmeyer lab for discussion. This work was supported by National Institutes
of Health grants ( R01-NS050580 to L.L. and F32MH096361 to L.A.S.). L.L. is an investigator
of HHMI. N.A. received support from FWF Firnberg-Programm ( T 1031 ). A.H.H. is
a recipient of a DOC Fellowship (24812) of the Austrian Academy of Sciences . This
work also received support from IST Austria institutional funds , FWF SFB F78 to
S.H., the People Programme (Marie Curie Actions) of the European Union’s Seventh
Framework Programme ( FP7/2007-2013 ) under REA grant agreement no 618444 to S.H.,
and the European Research Council (ERC) under the European Union’s Horizon 2020
Research and Innovation Programme (grant agreement no. 725780 LinPro ) to S.H.
article_number: '109274'
article_processing_charge: No
article_type: original
author:
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Amarbayasgalan
full_name: Davaatseren, Amarbayasgalan
id: 70ADC922-B424-11E9-99E3-BA18E6697425
last_name: Davaatseren
- first_name: Andi H
full_name: Hansen, Andi H
id: 38853E16-F248-11E8-B48F-1D18A9856A87
last_name: Hansen
- first_name: Johanna
full_name: Sonntag, Johanna
id: 32FE7D7C-F248-11E8-B48F-1D18A9856A87
last_name: Sonntag
- first_name: Lill
full_name: Andersen, Lill
last_name: Andersen
- first_name: Tina
full_name: Bernthaler, Tina
last_name: Bernthaler
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Anna-Magdalena
full_name: Heger, Anna-Magdalena
id: 4B76FFD2-F248-11E8-B48F-1D18A9856A87
last_name: Heger
- first_name: Randy L.
full_name: Johnson, Randy L.
last_name: Johnson
- first_name: Lindsay A.
full_name: Schwarz, Lindsay A.
last_name: Schwarz
- first_name: Liqun
full_name: Luo, Liqun
last_name: Luo
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Contreras X, Amberg N, Davaatseren A, et al. A genome-wide library of MADM
mice for single-cell genetic mosaic analysis. Cell Reports. 2021;35(12).
doi:10.1016/j.celrep.2021.109274
apa: Contreras, X., Amberg, N., Davaatseren, A., Hansen, A. H., Sonntag, J., Andersen,
L., … Hippenmeyer, S. (2021). A genome-wide library of MADM mice for single-cell
genetic mosaic analysis. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2021.109274
chicago: Contreras, Ximena, Nicole Amberg, Amarbayasgalan Davaatseren, Andi H Hansen,
Johanna Sonntag, Lill Andersen, Tina Bernthaler, et al. “A Genome-Wide Library
of MADM Mice for Single-Cell Genetic Mosaic Analysis.” Cell Reports. Cell
Press, 2021. https://doi.org/10.1016/j.celrep.2021.109274.
ieee: X. Contreras et al., “A genome-wide library of MADM mice for single-cell
genetic mosaic analysis,” Cell Reports, vol. 35, no. 12. Cell Press, 2021.
ista: Contreras X, Amberg N, Davaatseren A, Hansen AH, Sonntag J, Andersen L, Bernthaler
T, Streicher C, Heger A-M, Johnson RL, Schwarz LA, Luo L, Rülicke T, Hippenmeyer
S. 2021. A genome-wide library of MADM mice for single-cell genetic mosaic analysis.
Cell Reports. 35(12), 109274.
mla: Contreras, Ximena, et al. “A Genome-Wide Library of MADM Mice for Single-Cell
Genetic Mosaic Analysis.” Cell Reports, vol. 35, no. 12, 109274, Cell Press,
2021, doi:10.1016/j.celrep.2021.109274.
short: X. Contreras, N. Amberg, A. Davaatseren, A.H. Hansen, J. Sonntag, L. Andersen,
T. Bernthaler, C. Streicher, A.-M. Heger, R.L. Johnson, L.A. Schwarz, L. Luo,
T. Rülicke, S. Hippenmeyer, Cell Reports 35 (2021).
date_created: 2021-06-27T22:01:48Z
date_published: 2021-06-22T00:00:00Z
date_updated: 2026-04-02T14:04:28Z
day: '22'
ddc:
- '570'
department:
- _id: SiHi
- _id: LoSw
- _id: PreCl
doi: 10.1016/j.celrep.2021.109274
ec_funded: 1
external_id:
isi:
- '000664463600016'
pmid:
- '34161767'
file:
- access_level: open_access
checksum: d49520fdcbbb5c2f883bddb67cee5d77
content_type: application/pdf
creator: asandaue
date_created: 2021-06-28T14:06:24Z
date_updated: 2021-06-28T14:06:24Z
file_id: '9613'
file_name: 2021_CellReports_Contreras.pdf
file_size: 7653149
relation: main_file
success: 1
file_date_updated: 2021-06-28T14:06:24Z
has_accepted_license: '1'
intvolume: ' 35'
isi: 1
issue: '12'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2625A13E-B435-11E9-9278-68D0E5697425
grant_number: '24812'
name: Molecular mechanisms of radial neuronal migration
- _id: 25D61E48-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '618444'
name: Molecular Mechanisms of Cerebral Cortex Development
- _id: 260018B0-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '725780'
name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/boost-for-mouse-genetic-analysis/
scopus_import: '1'
status: public
title: A genome-wide library of MADM mice for single-cell genetic mosaic analysis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 35
year: '2021'
...
---
_id: '8914'
abstract:
- lang: eng
text: Amyotrophic lateral sclerosis (ALS) leads to a loss of specific motor neuron
populations in the spinal cord and cortex. Emerging evidence suggests that interneurons
may also be affected, but a detailed characterization of interneuron loss and
its potential impacts on motor neuron loss and disease progression is lacking.
To examine this issue, the fate of V1 inhibitory neurons during ALS was assessed
in the ventral spinal cord using the SODG93A mouse model. The V1 population makes
up ∼30% of all ventral inhibitory neurons, ∼50% of direct inhibitory synaptic
contacts onto motor neuron cell bodies, and is thought to play a key role in modulating
motor output, in part through recurrent and reciprocal inhibitory circuits. We
find that approximately half of V1 inhibitory neurons are lost in SODG93A mice
at late disease stages, but that this loss is delayed relative to the loss of
motor neurons and V2a excitatory neurons. We further identify V1 subpopulations
based on transcription factor expression that are differentially susceptible to
degeneration in SODG93A mice. At an early disease stage, we show that V1 synaptic
contacts with motor neuron cell bodies increase, suggesting an upregulation of
inhibition before V1 neurons are lost in substantial numbers. These data support
a model in which progressive changes in V1 synaptic contacts early in disease,
and in select V1 subpopulations at later stages, represent a compensatory upregulation
and then deleterious breakdown of specific interneuron circuits within the spinal
cord.
acknowledgement: This work was made possible by the generous support of Project ALS.
Imaging and related analyses were facilitated by The Waitt Advanced Biophotonics
Center Core at the Salk Institute, supported by grants from NIH-NCI CCSG (P30 014195)
and NINDS Neuroscience Center (NS072031). The authors would like to additionally
thank Drs. Jane Dodd, Robert Brownstone, and Laskaro Zagoraiou for helpful comments
on the manuscript. This manuscript is dedicated to Tom Jessell, an inspirational
scientist, friend and mentor.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alina
full_name: Salamatina, Alina
last_name: Salamatina
- first_name: Jerry H
full_name: Yang, Jerry H
last_name: Yang
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: 'Jay B '
full_name: 'Bikoff, Jay B '
last_name: Bikoff
- first_name: Linjing
full_name: Fang, Linjing
last_name: Fang
- first_name: Christopher R
full_name: Kintner, Christopher R
last_name: Kintner
- first_name: Thomas M
full_name: Jessell, Thomas M
last_name: Jessell
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Salamatina A, Yang JH, Brenner-Morton S, et al. Differential loss of spinal
interneurons in a mouse model of ALS. Neuroscience. 2020;450:81-95. doi:10.1016/j.neuroscience.2020.08.011
apa: Salamatina, A., Yang, J. H., Brenner-Morton, S., Bikoff, J. B., Fang, L., Kintner,
C. R., … Sweeney, L. B. (2020). Differential loss of spinal interneurons in a
mouse model of ALS. Neuroscience. Elsevier. https://doi.org/10.1016/j.neuroscience.2020.08.011
chicago: Salamatina, Alina, Jerry H Yang, Susan Brenner-Morton, Jay B Bikoff, Linjing
Fang, Christopher R Kintner, Thomas M Jessell, and Lora B. Sweeney. “Differential
Loss of Spinal Interneurons in a Mouse Model of ALS.” Neuroscience. Elsevier,
2020. https://doi.org/10.1016/j.neuroscience.2020.08.011.
ieee: A. Salamatina et al., “Differential loss of spinal interneurons in
a mouse model of ALS,” Neuroscience, vol. 450. Elsevier, pp. 81–95, 2020.
ista: Salamatina A, Yang JH, Brenner-Morton S, Bikoff JB, Fang L, Kintner CR, Jessell
TM, Sweeney LB. 2020. Differential loss of spinal interneurons in a mouse model
of ALS. Neuroscience. 450, 81–95.
mla: Salamatina, Alina, et al. “Differential Loss of Spinal Interneurons in a Mouse
Model of ALS.” Neuroscience, vol. 450, Elsevier, 2020, pp. 81–95, doi:10.1016/j.neuroscience.2020.08.011.
short: A. Salamatina, J.H. Yang, S. Brenner-Morton, J.B. Bikoff, L. Fang, C.R. Kintner,
T.M. Jessell, L.B. Sweeney, Neuroscience 450 (2020) 81–95.
corr_author: '1'
date_created: 2020-12-03T11:47:31Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2024-10-09T21:00:14Z
day: '01'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1016/j.neuroscience.2020.08.011
external_id:
isi:
- '000595588700008'
pmid:
- '32858144'
file:
- access_level: open_access
checksum: da7413c819e079720669c82451b49294
content_type: application/pdf
creator: dernst
date_created: 2020-12-03T11:45:26Z
date_updated: 2020-12-03T11:45:26Z
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file_name: 2020_Neuroscience_Salamatina.pdf
file_size: 4071247
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has_accepted_license: '1'
intvolume: ' 450'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 81-95
pmid: 1
publication: Neuroscience
publication_identifier:
issn:
- 0306-4522
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential loss of spinal interneurons in a mouse model of ALS
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 450
year: '2020'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19544'
abstract:
- lang: eng
text: Medicinal bioinorganic chemistry is a thriving field of drug research for
cancer treatment. Transition metal complexes coordinated to essential biological
scaffolds represent a highly promising class of compounds for design of novel
target-specific therapeutics. We report here the biological evaluation of a novel
Isatin-Schiff base derivative and its Cu(II) complex in several tumor cell lines
by assessing their effects on cellular metabolism, real-time cell proliferation
and induction of apoptosis. Further, the impact of compounds on the p53 protein
and expression of its target genes, including MDM2, p21/CDKN1A, and PUMA was evaluated.
Results obtained in this study provide further evidence in support of our prior
data suggesting the p53-mediated mechanism of action for Isatin-Schiff base derivatives
and their complexes and also shed light on potential use of these compounds for
stimulation of apoptosis in breast cancer cells via activation of the pro-apoptotic
PUMA gene.
article_number: '103'
article_processing_charge: Yes
article_type: original
author:
- first_name: Emil
full_name: Bulatov, Emil
last_name: Bulatov
- first_name: Regina
full_name: Sayarova, Regina
last_name: Sayarova
- first_name: Rimma
full_name: Mingaleeva, Rimma
last_name: Mingaleeva
- first_name: Regina
full_name: Miftakhova, Regina
last_name: Miftakhova
- first_name: Marina
full_name: Gomzikova, Marina
last_name: Gomzikova
- first_name: Iurii
full_name: Ignatev, Iurii
id: 2ac71786-dc7d-11ea-9b2f-c5ad4b9faff6
last_name: Ignatev
- first_name: Alexey
full_name: Petukhov, Alexey
last_name: Petukhov
- first_name: Pavel
full_name: Davidovich, Pavel
last_name: Davidovich
- first_name: Albert
full_name: Rizvanov, Albert
last_name: Rizvanov
- first_name: Nickolai A.
full_name: Barlev, Nickolai A.
last_name: Barlev
citation:
ama: Bulatov E, Sayarova R, Mingaleeva R, et al. Isatin-Schiff base-copper (II)
complex induces cell death in p53-positive tumors. Cell Death Discovery.
2018;4. doi:10.1038/s41420-018-0120-z
apa: Bulatov, E., Sayarova, R., Mingaleeva, R., Miftakhova, R., Gomzikova, M., Ignatev,
I., … Barlev, N. A. (2018). Isatin-Schiff base-copper (II) complex induces cell
death in p53-positive tumors. Cell Death Discovery. Springer Nature. https://doi.org/10.1038/s41420-018-0120-z
chicago: Bulatov, Emil, Regina Sayarova, Rimma Mingaleeva, Regina Miftakhova, Marina
Gomzikova, Iurii Ignatev, Alexey Petukhov, Pavel Davidovich, Albert Rizvanov,
and Nickolai A. Barlev. “Isatin-Schiff Base-Copper (II) Complex Induces Cell Death
in P53-Positive Tumors.” Cell Death Discovery. Springer Nature, 2018. https://doi.org/10.1038/s41420-018-0120-z.
ieee: E. Bulatov et al., “Isatin-Schiff base-copper (II) complex induces
cell death in p53-positive tumors,” Cell Death Discovery, vol. 4. Springer
Nature, 2018.
ista: Bulatov E, Sayarova R, Mingaleeva R, Miftakhova R, Gomzikova M, Ignatev I,
Petukhov A, Davidovich P, Rizvanov A, Barlev NA. 2018. Isatin-Schiff base-copper
(II) complex induces cell death in p53-positive tumors. Cell Death Discovery.
4, 103.
mla: Bulatov, Emil, et al. “Isatin-Schiff Base-Copper (II) Complex Induces Cell
Death in P53-Positive Tumors.” Cell Death Discovery, vol. 4, 103, Springer
Nature, 2018, doi:10.1038/s41420-018-0120-z.
short: E. Bulatov, R. Sayarova, R. Mingaleeva, R. Miftakhova, M. Gomzikova, I. Ignatev,
A. Petukhov, P. Davidovich, A. Rizvanov, N.A. Barlev, Cell Death Discovery 4 (2018).
date_created: 2025-04-11T01:31:42Z
date_published: 2018-11-13T00:00:00Z
date_updated: 2025-07-10T11:51:52Z
day: '13'
ddc:
- '570'
department:
- _id: GradSch
- _id: LoSw
doi: 10.1038/s41420-018-0120-z
extern: '1'
external_id:
pmid:
- '30455989 '
has_accepted_license: '1'
intvolume: ' 4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41420-018-0120-z
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Death Discovery
publication_identifier:
issn:
- 2058-7716
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2018'
...