[{"day":"10","file":[{"date_created":"2025-06-04T05:43:27Z","file_id":"19790","checksum":"a83a4cb58f5941096d3ad91ca0172594","success":1,"file_name":"2025_DevelopmentalCell_Jaeger.pdf","file_size":11936258,"date_updated":"2025-06-04T05:43:27Z","creator":"dernst","access_level":"open_access","content_type":"application/pdf","relation":"main_file"}],"corr_author":"1","issue":"5","oa_version":"Published Version","date_updated":"2025-09-30T10:00:55Z","oa":1,"language":[{"iso":"eng"}],"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"OA_type":"hybrid","month":"03","pmid":1,"doi":"10.1016/j.devcel.2024.10.025","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","abstract":[{"lang":"eng","text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution."}],"ddc":["570"],"status":"public","date_published":"2025-03-10T00:00:00Z","publisher":"Elsevier","intvolume":"        60","type":"journal_article","isi":1,"citation":{"mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>.","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","ieee":"E. C. B. Jaeger <i>et al.</i>, “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” <i>Developmental Cell</i>, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. 2025;60(5):794-812.e6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.","chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>."},"has_accepted_license":"1","scopus_import":"1","quality_controlled":"1","author":[{"last_name":"Jaeger","first_name":"Eliza C.B.","full_name":"Jaeger, Eliza C.B."},{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic","first_name":"David","full_name":"Vijatovic, David"},{"full_name":"Deryckere, Astrid","first_name":"Astrid","last_name":"Deryckere"},{"last_name":"Zorin","first_name":"Nikol","full_name":"Zorin, Nikol"},{"last_name":"Nguyen","first_name":"Akemi L.","full_name":"Nguyen, Akemi L."},{"first_name":"Georgiy","id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","last_name":"Ivanian","full_name":"Ivanian, Georgiy"},{"last_name":"Woych","first_name":"Jamie","full_name":"Woych, Jamie"},{"first_name":"Rebecca C","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f","last_name":"Arnold","full_name":"Arnold, Rebecca C"},{"full_name":"Ortega Gurrola, Alonso","last_name":"Ortega Gurrola","first_name":"Alonso"},{"full_name":"Shvartsman, Arik","first_name":"Arik","last_name":"Shvartsman"},{"last_name":"Barbieri","id":"a9492887-8972-11ed-ae7b-bfae10998254","first_name":"Francesca","full_name":"Barbieri, Francesca"},{"full_name":"Toma, Florina-Alexandra","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5","last_name":"Toma","first_name":"Florina-Alexandra"},{"last_name":"Gorbsky","first_name":"Gary J.","full_name":"Gorbsky, Gary J."},{"full_name":"Horb, Marko E.","first_name":"Marko E.","last_name":"Horb"},{"full_name":"Cline, Hollis T.","last_name":"Cline","first_name":"Hollis T."},{"first_name":"Timothy F.","last_name":"Shay","full_name":"Shay, Timothy F."},{"full_name":"Kelley, Darcy B.","first_name":"Darcy B.","last_name":"Kelley"},{"full_name":"Yamaguchi, Ayako","first_name":"Ayako","last_name":"Yamaguchi"},{"full_name":"Shein-Idelson, Mark","last_name":"Shein-Idelson","first_name":"Mark"},{"full_name":"Tosches, Maria Antonietta","last_name":"Tosches","first_name":"Maria Antonietta"},{"full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger"}],"_id":"15016","file_date_updated":"2025-06-04T05:43:27Z","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"date_created":"2024-02-20T09:20:32Z","acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","article_processing_charge":"Yes (via OA deal)","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"OA_place":"publisher","page":"794-812.e6","year":"2025","publication":"Developmental Cell","project":[{"grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"},{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"}],"external_id":{"pmid":["39603234"],"isi":["001444798600001"]},"article_type":"original","title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","volume":60},{"intvolume":"        16","PlanS_conform":"1","publisher":"Springer Nature","type":"journal_article","DOAJ_listed":"1","has_accepted_license":"1","quality_controlled":"1","article_number":"11355","scopus_import":"1","citation":{"chicago":"Artan, Murat, Hanna Schön, and Mario de Bono. “Proximity Labeling of DAF-16 FOXO Highlights Aging Regulatory Proteins.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66409-0\">https://doi.org/10.1038/s41467-025-66409-0</a>.","short":"M. Artan, H. Schön, M. de Bono, Nature Communications 16 (2025).","apa":"Artan, M., Schön, H., &#38; de Bono, M. (2025). Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66409-0\">https://doi.org/10.1038/s41467-025-66409-0</a>","ama":"Artan M, Schön H, de Bono M. Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66409-0\">10.1038/s41467-025-66409-0</a>","ieee":"M. Artan, H. Schön, and M. de Bono, “Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","ista":"Artan M, Schön H, de Bono M. 2025. Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins. Nature Communications. 16, 11355.","mla":"Artan, Murat, et al. “Proximity Labeling of DAF-16 FOXO Highlights Aging Regulatory Proteins.” <i>Nature Communications</i>, vol. 16, 11355, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66409-0\">10.1038/s41467-025-66409-0</a>."},"author":[{"last_name":"Artan","id":"C407B586-6052-11E9-B3AE-7006E6697425","orcid":"0000-0001-8945-6992","first_name":"Murat","full_name":"Artan, Murat"},{"full_name":"Schön, Hanna","id":"C8E17EDC-D7AA-11E9-B7B7-45ECE5697425","last_name":"Schön","first_name":"Hanna"},{"full_name":"De Bono, Mario","first_name":"Mario","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"De Bono","orcid":"0000-0001-8347-0443"}],"file_date_updated":"2026-01-05T10:58:28Z","_id":"20929","date_created":"2026-01-04T23:01:34Z","acknowledgement":"We thank de Bono lab members for helpful comments on the manuscript, and the Mass Spec Facility at the Max Perutz Labs, notably WeiQiang Chen and Markus Hartl, for invaluable discussions and comments on mass spec analyses of worm samples. All LC-MS/MS analyses were performed on instruments of the Vienna BioCenter Core Facilities (VBCF). Microscopy was supported by the Scientific Services Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF). We are grateful to Dr. Geraldine Seydoux (Johns Hopkins University) for worm strains and plasmids, and Dr. Seung-Jae V. Lee (KAIST) for RNAi clones. We are grateful to Ekaterina Lashmanova for designing the daf-16::TbID::mNG::3xFLAG knock-in construct and for her outstanding support in the lab. This work was supported by a Wellcome Investigator Award (209504/A/17/Z) to MdB and an ISTplus Fellowship to MA (Marie Sklodowska-Curie agreement No 754411).","article_processing_charge":"Yes","acknowledged_ssus":[{"_id":"Bio"}],"publication_identifier":{"eissn":["2041-1723"]},"department":[{"_id":"MaDe"}],"OA_place":"publisher","external_id":{"pmid":["41381452"]},"title":"Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins","article_type":"original","volume":16,"year":"2025","project":[{"call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"Molecular mechanisms of neural circuit function","_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","grant_number":"209504/A/17/Z"}],"publication":"Nature Communications","day":"11","corr_author":"1","file":[{"file_id":"20941","date_created":"2026-01-05T10:58:28Z","checksum":"748e2e003b878b85b6048d51621d6aae","file_name":"2025_NatureComm_Artan.pdf","file_size":1642352,"success":1,"date_updated":"2026-01-05T10:58:28Z","access_level":"open_access","creator":"dernst","relation":"main_file","content_type":"application/pdf"}],"date_updated":"2026-01-05T11:00:03Z","oa_version":"Published Version","language":[{"iso":"eng"}],"oa":1,"ec_funded":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"12","OA_type":"gold","pmid":1,"doi":"10.1038/s41467-025-66409-0","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"Insulin/insulin-like growth factor signaling inhibits FOXO transcription factors to control development, homeostasis, and aging. Here, we use proximity labeling to identify proteins interacting with the C. elegans FOXO DAF-16. We show that in well-fed, unstressed animals harboring active insulin signaling, DAF-16 forms a complex with the PAR-1/MARK serine/threonine kinase, a key regulator of cell polarity. PAR-1 inhibits DAF-16 accumulation and promotes DAF-16 phosphorylation at S249, at a conserved motif that PAR-1/human MARK2 phosphorylates in vitro. DAF-2 insulin-like receptor signaling stimulates DAF-16 S249 phosphorylation, suggesting DAF-2 activates PAR-1. DAF-2 also promotes PAR-1 expression by inhibiting DAF-16. PAR-1 knockdown, or DAF-16 S249A, prolong lifespan, whereas phosphomimetic DAF-16 S249D suppresses the longevity of daf-2 mutants. At low insulin signaling, DAF-16 proximity labeling highlights transcription factors, chromatin regulators, and DNA repair proteins. One interactor, the zinc finger/homeobox protein ZFH-2/ZFHX3, forms a complex with DAF-16 and prolongs lifespan. Our work provides entry points for hypothesis-driven studies of FOXO function and longevity.","lang":"eng"}],"publication_status":"published","ddc":["570"],"date_published":"2025-12-11T00:00:00Z","status":"public"},{"_id":"20167","file_date_updated":"2025-09-18T14:12:29Z","author":[{"full_name":"Schön, Hanna","id":"C8E17EDC-D7AA-11E9-B7B7-45ECE5697425","last_name":"Schön","first_name":"Hanna"}],"has_accepted_license":"1","citation":{"ama":"Schön H. The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20167\">10.15479/AT-ISTA-20167</a>","ista":"Schön H. 2025. The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs. Institute of Science and Technology Austria.","ieee":"H. Schön, “The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs,” Institute of Science and Technology Austria, 2025.","mla":"Schön, Hanna. <i>The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20167\">10.15479/AT-ISTA-20167</a>.","chicago":"Schön, Hanna. “The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20167\">https://doi.org/10.15479/AT-ISTA-20167</a>.","short":"H. Schön, The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs, Institute of Science and Technology Austria, 2025.","apa":"Schön, H. (2025). <i>The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20167\">https://doi.org/10.15479/AT-ISTA-20167</a>"},"type":"dissertation","publisher":"Institute of Science and Technology Austria","title":"The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs","project":[{"name":"Molecular mechanisms of neural circuit function","_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","grant_number":"209504/A/17/Z"},{"grant_number":"ALTF 302-2019","_id":"23813290-32DE-11EA-91FC-C7463DDC885E","name":"Control of gene expression at the endoplasmic reticulum"}],"year":"2025","degree_awarded":"PhD","OA_place":"publisher","page":"171","department":[{"_id":"GradSch"},{"_id":"MaDe"}],"article_processing_charge":"No","date_created":"2025-08-13T11:13:13Z","acknowledgement":"This work was supported by EMBO (ALTF 302-2019 to Niko Amin-Wetzel), the FWF\r\n(ESPRIT PR1054E140 to Niko Amin-Wetzel), the European Research Council\r\n(Advanced Grant 269058 to Mario de Bono) and Wellcome (209504/A/17/Z\r\nInvestigator Award to Mario de Bono). ","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication_identifier":{"isbn":["978-3-99078-061-9"],"issn":["2663-337X"]},"language":[{"iso":"eng"}],"oa_version":"Published Version","date_updated":"2026-04-07T11:50:26Z","alternative_title":["ISTA Thesis"],"corr_author":"1","file":[{"access_level":"closed","creator":"hschoen","date_updated":"2025-09-09T08:57:04Z","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"b40c74404b8d9593802dabf57bfdf10f","file_id":"20311","date_created":"2025-09-08T14:33:50Z","file_name":"2025_Schoen_Hanna_Thesis.docx","file_size":78812587},{"file_size":9667057,"file_name":"2025_Schoen_Hanna_Thesis.pdf","date_created":"2025-09-11T14:20:59Z","file_id":"20347","checksum":"16abc3ff66396ce2457fe07ffa8bed90","content_type":"application/pdf","relation":"main_file","embargo_to":"open_access","embargo":"2026-09-15","date_updated":"2025-09-18T14:12:29Z","creator":"hschoen","access_level":"closed"}],"day":"13","date_published":"2025-08-13T00:00:00Z","status":"public","ddc":["570"],"publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","doi":"10.15479/AT-ISTA-20167","supervisor":[{"id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"de Bono","orcid":"0000-0001-8347-0443","first_name":"Mario","full_name":"de Bono, Mario"}],"month":"08","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"supervisor":[{"full_name":"de Bono, Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"de Bono","first_name":"Mario"}],"doi":"10.15479/AT-ISTA-20485","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"10","date_published":"2025-10-23T00:00:00Z","status":"public","publication_status":"published","ddc":["570"],"corr_author":"1","file":[{"relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","creator":"mmisova","date_updated":"2025-11-06T11:08:06Z","file_name":"2025-Misova-Michaela-Thesis.docx","file_size":75070995,"checksum":"e042ea314e7e13fce76c6c95e126779a","file_id":"20518","date_created":"2025-10-23T08:22:35Z"},{"date_created":"2025-10-23T08:21:21Z","file_id":"20519","checksum":"fcd8973d6a025256eb0eb1a82c02172c","file_name":"2025-Misova-Michaela-Thesis.pdf","file_size":10974630,"embargo":"2026-10-23","date_updated":"2025-10-23T08:21:21Z","creator":"mmisova","access_level":"closed","content_type":"application/pdf","relation":"main_file","embargo_to":"open_access"}],"day":"23","language":[{"iso":"eng"}],"ec_funded":1,"oa_version":"Published Version","alternative_title":["ISTA Thesis"],"date_updated":"2026-04-07T11:54:00Z","department":[{"_id":"GradSch"},{"_id":"MaDe"}],"date_created":"2025-10-17T16:15:09Z","acknowledgement":"I would also like to acknowledge the funding that I received from the European Union’s\r\nHorizon 2020 research and Innovation programme under the Marie Sklodowska-Curie\r\nGrant Agreement No. 665385. This work would not have been possible without the contribution and support of people\r\nbehind the scientific service units at ISTA: the Life Science Facility (LSF), Imaging and\r\nOptics Facility (IOF), the Bioinformatics Unit, Protein Services Unit and\r\nElectrophysiology Unit. I would also like to recognize the work of people at the Vienna\r\nBiocenter (VBC) Mass Spectrometry Facility, particularly Markus Hartl and WeiQiang\r\nChen. ","article_processing_charge":"No","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"publication_identifier":{"isbn":["978-3-99078-068-8"],"issn":["2663-337X"]},"title":"Dissecting gap junction biology using the C. elegans nervous system","year":"2025","project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","grant_number":"665385","call_identifier":"H2020"}],"page":"155","OA_place":"publisher","degree_awarded":"PhD","type":"dissertation","publisher":"Institute of Science and Technology Austria","author":[{"last_name":"Misova","id":"495A3C32-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2427-6856","first_name":"Michaela","full_name":"Misova, Michaela"}],"file_date_updated":"2025-11-06T11:08:06Z","_id":"20485","has_accepted_license":"1","citation":{"chicago":"Misova, Michaela. “Dissecting Gap Junction Biology Using the C. Elegans Nervous System.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20485\">https://doi.org/10.15479/AT-ISTA-20485</a>.","apa":"Misova, M. (2025). <i>Dissecting gap junction biology using the C. elegans nervous system</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20485\">https://doi.org/10.15479/AT-ISTA-20485</a>","short":"M. Misova, Dissecting Gap Junction Biology Using the C. Elegans Nervous System, Institute of Science and Technology Austria, 2025.","ama":"Misova M. Dissecting gap junction biology using the C. elegans nervous system. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20485\">10.15479/AT-ISTA-20485</a>","mla":"Misova, Michaela. <i>Dissecting Gap Junction Biology Using the C. Elegans Nervous System</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20485\">10.15479/AT-ISTA-20485</a>.","ieee":"M. Misova, “Dissecting gap junction biology using the C. elegans nervous system,” Institute of Science and Technology Austria, 2025.","ista":"Misova M. 2025. Dissecting gap junction biology using the C. elegans nervous system. Institute of Science and Technology Austria."}},{"file_date_updated":"2025-09-27T22:30:02Z","_id":"19498","author":[{"last_name":"Stratigi","first_name":"Aikaterini","full_name":"Stratigi, Aikaterini"},{"last_name":"Soler-García","first_name":"Miguel","full_name":"Soler-García, Miguel"},{"first_name":"Mia","last_name":"Krout","full_name":"Krout, Mia"},{"last_name":"Shukla","first_name":"Shikha","full_name":"Shukla, Shikha"},{"full_name":"De Bono, Mario","first_name":"Mario","last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443"},{"first_name":"Janet E.","last_name":"Richmond","full_name":"Richmond, Janet E."},{"full_name":"Laurent, Patrick","first_name":"Patrick","last_name":"Laurent"}],"article_number":"e1767232024","quality_controlled":"1","scopus_import":"1","has_accepted_license":"1","citation":{"mla":"Stratigi, Aikaterini, et al. “Neuroendocrine Control of Synaptic Transmission by PHAC-1 in C. Elegans.” <i>Journal of Neuroscience</i>, vol. 45, no. 13, e1767232024, Society for Neuroscience, 2025, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1767-23.2024\">10.1523/JNEUROSCI.1767-23.2024</a>.","ista":"Stratigi A, Soler-García M, Krout M, Shukla S, de Bono M, Richmond JE, Laurent P. 2025. Neuroendocrine control of synaptic transmission by PHAC-1 in C. elegans. Journal of Neuroscience. 45(13), e1767232024.","ieee":"A. Stratigi <i>et al.</i>, “Neuroendocrine control of synaptic transmission by PHAC-1 in C. elegans,” <i>Journal of Neuroscience</i>, vol. 45, no. 13. Society for Neuroscience, 2025.","ama":"Stratigi A, Soler-García M, Krout M, et al. Neuroendocrine control of synaptic transmission by PHAC-1 in C. elegans. <i>Journal of Neuroscience</i>. 2025;45(13). doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1767-23.2024\">10.1523/JNEUROSCI.1767-23.2024</a>","apa":"Stratigi, A., Soler-García, M., Krout, M., Shukla, S., de Bono, M., Richmond, J. E., &#38; Laurent, P. (2025). Neuroendocrine control of synaptic transmission by PHAC-1 in C. elegans. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.1767-23.2024\">https://doi.org/10.1523/JNEUROSCI.1767-23.2024</a>","short":"A. Stratigi, M. Soler-García, M. Krout, S. Shukla, M. de Bono, J.E. Richmond, P. Laurent, Journal of Neuroscience 45 (2025).","chicago":"Stratigi, Aikaterini, Miguel Soler-García, Mia Krout, Shikha Shukla, Mario de Bono, Janet E. Richmond, and Patrick Laurent. “Neuroendocrine Control of Synaptic Transmission by PHAC-1 in C. Elegans.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2025. <a href=\"https://doi.org/10.1523/JNEUROSCI.1767-23.2024\">https://doi.org/10.1523/JNEUROSCI.1767-23.2024</a>."},"isi":1,"type":"journal_article","intvolume":"        45","publisher":"Society for Neuroscience","volume":45,"article_type":"original","title":"Neuroendocrine control of synaptic transmission by PHAC-1 in C. elegans","external_id":{"pmid":["39919830"],"isi":["001460952700001"]},"publication":"Journal of Neuroscience","year":"2025","OA_place":"publisher","department":[{"_id":"MaDe"}],"article_processing_charge":"No","acknowledgement":"P.L. is a research associate of the Belgian National Fund for Scientific Research (FRS-FNRS). K.S., M.S.-G., S.S., and P.L. are supported by grants from the FRS-FNRS. This work was supported by an Advanced ERC Grant (269058 ACMO) to M.D.B. We thank the team of Alexander Gottschalk for the snn-1(S9A) strain. We thank the Imaging Facility of the Faculty of Medicine (LiMiF) of the Universite Libre de Bruxelles, supported by FRS-FNRS. This work made use of instruments in the Electron Microscopy Core of the University of Illinois Chicago Research Resources Center as well as the BioCryo facility of Northwestern University's NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern's MRSEC program (NSF DMR-2308691). Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).","date_created":"2025-04-06T22:01:32Z","publication_identifier":{"issn":["0270-6474"],"eissn":["1529-2401"]},"language":[{"iso":"eng"}],"oa":1,"oa_version":"Published Version","date_updated":"2025-09-30T11:29:28Z","issue":"13","file":[{"access_level":"open_access","creator":"dernst","date_updated":"2025-09-27T22:30:02Z","embargo":"2025-09-27","relation":"main_file","content_type":"application/pdf","checksum":"7befc0168f4cd5bd2b0fcff9e2a94784","file_id":"19525","date_created":"2025-04-07T11:57:19Z","file_size":3111735,"file_name":"2025_JourNeuroscience_Stratigi.pdf"}],"day":"26","date_published":"2025-03-26T00:00:00Z","status":"public","ddc":["570"],"abstract":[{"lang":"eng","text":"A dynamic interplay between fast synaptic signals and slower neuromodulatory signals controls the excitatory/inhibitory (E/I) balance within neuronal circuits. The mechanisms by which neuropeptide signaling is regulated to maintain E/I balance remain uncertain. We designed a genetic screen to isolate genes involved in the peptidergic maintenance of the E/I balance in the C. elegans motor circuit. This screen identified the C. elegans orthologs of the presynaptic phosphoprotein synapsin (snn-1) and the protein phosphatase 1 (PP1) regulatory subunit PHACTR1 (phac-1). We demonstrate that both phac-1 and snn-1 alter the motor behavior of C. elegans, and genetic interactions suggest that SNN-1 contributes to PP1-PHAC-1 holoenzyme signaling. De novo variants of human PHACTR1, associated with early-onset epilepsies [developmental and epileptic encephalopathy 70 (DEE70)], when expressed in C. elegans resulted in constitutive PP1-PHAC-1 holoenzyme activity. Unregulated PP1-PHAC-1 signaling alters the synapsin and actin cytoskeleton and increases neuropeptide release by cholinergic motor neurons, which secondarily affects the presynaptic vesicle cycle. Together, these results clarify the dominant mechanisms of action of the DEE70 alleles and suggest that altered neuropeptide release may alter E/I balance in DEE70."}],"publication_status":"published","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1523/JNEUROSCI.1767-23.2024","pmid":1,"month":"03","OA_type":"hybrid","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"type":"book_chapter","intvolume":"       181","editor":[{"first_name":"Daisuke","last_name":"Yamamoto","full_name":"Yamamoto, Daisuke"}],"publisher":"Springer Nature","author":[{"full_name":"Artan, Murat","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan","orcid":"0000-0001-8945-6992","first_name":"Murat"},{"full_name":"de Bono, Mario","first_name":"Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"de Bono"}],"_id":"11456","quality_controlled":"1","scopus_import":"1","citation":{"apa":"Artan, M., &#38; de Bono, M. (2022). Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling. In D. Yamamoto (Ed.), <i>Behavioral Neurogenetics</i> (Vol. 181, pp. 277–294). New York: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-2321-3_15\">https://doi.org/10.1007/978-1-0716-2321-3_15</a>","short":"M. Artan, M. de Bono, in:, D. Yamamoto (Ed.), Behavioral Neurogenetics, Springer Nature, New York, 2022, pp. 277–294.","chicago":"Artan, Murat, and Mario de Bono. “Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling.” In <i>Behavioral Neurogenetics</i>, edited by Daisuke Yamamoto, 181:277–94. NM. New York: Springer Nature, 2022. <a href=\"https://doi.org/10.1007/978-1-0716-2321-3_15\">https://doi.org/10.1007/978-1-0716-2321-3_15</a>.","ista":"Artan M, de Bono M. 2022.Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling. In: Behavioral Neurogenetics. Neuromethods, vol. 181, 277–294.","ieee":"M. Artan and M. de Bono, “Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling,” in <i>Behavioral Neurogenetics</i>, vol. 181, D. Yamamoto, Ed. New York: Springer Nature, 2022, pp. 277–294.","mla":"Artan, Murat, and Mario de Bono. “Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling.” <i>Behavioral Neurogenetics</i>, edited by Daisuke Yamamoto, vol. 181, Springer Nature, 2022, pp. 277–94, doi:<a href=\"https://doi.org/10.1007/978-1-0716-2321-3_15\">10.1007/978-1-0716-2321-3_15</a>.","ama":"Artan M, de Bono M. Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling. In: Yamamoto D, ed. <i>Behavioral Neurogenetics</i>. Vol 181. NM. New York: Springer Nature; 2022:277-294. doi:<a href=\"https://doi.org/10.1007/978-1-0716-2321-3_15\">10.1007/978-1-0716-2321-3_15</a>"},"department":[{"_id":"MaDe"}],"acknowledgement":"We thank de Bono lab members for the helpful comments on the manuscript. The biotin-auxotrophic E. coli strain MG1655bioB:kan was a generous gift from J. Cronan (University of Illinois) and was kindly sent to us by Jessica Feldman and Ariana Sanchez (Stanford University). dg398 pEntryslot2_mNeongreen::3XFLAG::stop and dg397 pEntryslot3_mNeongreen::3XFLAG::stop::unc-54 3’UTR entry vector were kindly sent by Dr. Dominique Glauser (University of Fribourg). This work was supported by an Advanced ERC Grant (269058 ACMO) and a Wellcome Investigator Award (209504/Z/17/Z) to MdB and an ISTplus Fellowship to MA (Marie Sklodowska-Curie agreement No 754411).","date_created":"2022-06-20T08:10:34Z","article_processing_charge":"No","publication_identifier":{"eisbn":["9781071623213"],"issn":["0893-2336"],"eissn":["1940-6045"],"isbn":["9781071623206"]},"title":"Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling","volume":181,"place":"New York","year":"2022","project":[{"_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","name":"Molecular mechanisms of neural circuit function","grant_number":"209504/A/17/Z"},{"grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"}],"publication":"Behavioral Neurogenetics","page":"277-294","corr_author":"1","day":"04","language":[{"iso":"eng"}],"ec_funded":1,"series_title":"NM","date_updated":"2025-04-14T07:43:58Z","oa_version":"None","alternative_title":["Neuromethods"],"doi":"10.1007/978-1-0716-2321-3_15","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06","date_published":"2022-06-04T00:00:00Z","status":"public","abstract":[{"text":"The proteomes of specialized structures, and the interactomes of proteins of interest, provide entry points to elucidate the functions of molecular machines. Here, we review a proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes. Although the focus is on C. elegans neurons, the method is applicable regardless of cell type. We describe detailed extraction procedures that solubilize the bulk of C. elegans proteins and highlight the importance of tagging endogenous genes, to ensure physiological expression levels. We review issues associated with non-specific background noise and the importance of appropriate controls. As proof of principle, we review our analysis of the interactome of a presynaptic active zone protein, ELKS-1. Our aim is to provide a detailed protocol for TurboID-based proximity labeling in C. elegans and to highlight its potential and its limitations to characterize protein complexes and subcellular compartments in this animal.","lang":"eng"}],"publication_status":"published"},{"citation":{"ama":"Zhao L, Fenk LA, Nilsson L, et al. ROS and cGMP signaling modulate persistent escape from hypoxia in Caenorhabditis elegans. <i>PLoS Biology</i>. 2022;20(6). doi:<a href=\"https://doi.org/10.1371/journal.pbio.3001684\">10.1371/journal.pbio.3001684</a>","ieee":"L. Zhao <i>et al.</i>, “ROS and cGMP signaling modulate persistent escape from hypoxia in Caenorhabditis elegans,” <i>PLoS Biology</i>, vol. 20, no. 6. Public Library of Science, 2022.","mla":"Zhao, Lina, et al. “ROS and CGMP Signaling Modulate Persistent Escape from Hypoxia in Caenorhabditis Elegans.” <i>PLoS Biology</i>, vol. 20, no. 6, e3001684, Public Library of Science, 2022, doi:<a href=\"https://doi.org/10.1371/journal.pbio.3001684\">10.1371/journal.pbio.3001684</a>.","ista":"Zhao L, Fenk LA, Nilsson L, Amin-Wetzel NP, Ramirez N, de Bono M, Chen C. 2022. ROS and cGMP signaling modulate persistent escape from hypoxia in Caenorhabditis elegans. PLoS Biology. 20(6), e3001684.","chicago":"Zhao, Lina, Lorenz A. Fenk, Lars Nilsson, Niko Paresh Amin-Wetzel, Nelson Ramirez, Mario de Bono, and Changchun Chen. “ROS and CGMP Signaling Modulate Persistent Escape from Hypoxia in Caenorhabditis Elegans.” <i>PLoS Biology</i>. Public Library of Science, 2022. <a href=\"https://doi.org/10.1371/journal.pbio.3001684\">https://doi.org/10.1371/journal.pbio.3001684</a>.","short":"L. Zhao, L.A. Fenk, L. Nilsson, N.P. Amin-Wetzel, N. Ramirez, M. de Bono, C. Chen, PLoS Biology 20 (2022).","apa":"Zhao, L., Fenk, L. A., Nilsson, L., Amin-Wetzel, N. P., Ramirez, N., de Bono, M., &#38; Chen, C. (2022). ROS and cGMP signaling modulate persistent escape from hypoxia in Caenorhabditis elegans. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.3001684\">https://doi.org/10.1371/journal.pbio.3001684</a>"},"quality_controlled":"1","scopus_import":"1","article_number":"e3001684","has_accepted_license":"1","file_date_updated":"2022-07-25T07:38:49Z","_id":"11637","author":[{"full_name":"Zhao, Lina","last_name":"Zhao","first_name":"Lina"},{"first_name":"Lorenz A.","last_name":"Fenk","full_name":"Fenk, Lorenz A."},{"first_name":"Lars","last_name":"Nilsson","full_name":"Nilsson, Lars"},{"full_name":"Amin-Wetzel, Niko Paresh","first_name":"Niko Paresh","id":"E95D3014-9D8C-11E9-9C80-D2F8E5697425","last_name":"Amin-Wetzel"},{"full_name":"Ramirez, Nelson","id":"39831956-E4FE-11E9-85DE-0DC7E5697425","last_name":"Ramirez","first_name":"Nelson"},{"last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443","first_name":"Mario","full_name":"De Bono, Mario"},{"full_name":"Chen, Changchun","last_name":"Chen","first_name":"Changchun"}],"publisher":"Public Library of Science","intvolume":"        20","isi":1,"type":"journal_article","publication":"PLoS Biology","project":[{"grant_number":"209504/A/17/Z","name":"Molecular mechanisms of neural circuit function","_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E"}],"year":"2022","volume":20,"article_type":"original","title":"ROS and cGMP signaling modulate persistent escape from hypoxia in Caenorhabditis elegans","external_id":{"pmid":["35727855"],"isi":["000828679600001"]},"publication_identifier":{"eissn":["1545-7885"]},"article_processing_charge":"No","date_created":"2022-07-24T22:01:42Z","acknowledgement":" This work was funded by H2020 European Research Council (ERC Advanced grant, 269058 ACMO, https://erc.europa.eu/funding/advanced-grants) and Wellcome Trust UK (Wellcome Investigator Award, 209504/Z/17/Z, https://wellcome.org/grant-funding/people-and-projects/grants-awarded/molecular-mechanisms-neural-circuit-function-0) to M.d.B, and by H2020 European Research Council (ERC starting grant, 802653 OXYGEN SENSING, https://erc.europa.eu/funding/starting-grants) and Vetenskapsrådet (VR starting grant, 2018-02216, https://www.vr.se/english.html) to C.C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","department":[{"_id":"MaDe"}],"oa_version":"Published Version","date_updated":"2025-04-15T07:32:21Z","issue":"6","oa":1,"language":[{"iso":"eng"}],"day":"21","file":[{"checksum":"df4902f854ad76769d3203bfdc69f16c","file_id":"11643","date_created":"2022-07-25T07:38:49Z","file_size":3721585,"file_name":"2022_PLoSBiology_Zhao.pdf","success":1,"access_level":"open_access","creator":"dernst","date_updated":"2022-07-25T07:38:49Z","relation":"main_file","content_type":"application/pdf"}],"corr_author":"1","ddc":["570"],"abstract":[{"text":"The ability to detect and respond to acute oxygen (O2) shortages is indispensable to aerobic life. The molecular mechanisms and circuits underlying this capacity are poorly understood. Here, we characterize the behavioral responses of feeding Caenorhabditis elegans to approximately 1% O2. Acute hypoxia triggers a bout of turning maneuvers followed by a persistent switch to rapid forward movement as animals seek to avoid and escape hypoxia. While the behavioral responses to 1% O2 closely resemble those evoked by 21% O2, they have distinct molecular and circuit underpinnings. Disrupting phosphodiesterases (PDEs), specific G proteins, or BBSome function inhibits escape from 1% O2 due to increased cGMP signaling. A primary source of cGMP is GCY-28, the ortholog of the atrial natriuretic peptide (ANP) receptor. cGMP activates the protein kinase G EGL-4 and enhances neuroendocrine secretion to inhibit acute responses to 1% O2. Triggering a rise in cGMP optogenetically in multiple neurons, including AIA interneurons, rapidly and reversibly inhibits escape from 1% O2. Ca2+ imaging reveals that a 7% to 1% O2 stimulus evokes a Ca2+ decrease in several neurons. Defects in mitochondrial complex I (MCI) and mitochondrial complex I (MCIII), which lead to persistently high reactive oxygen species (ROS), abrogate acute hypoxia responses. In particular, repressing the expression of isp-1, which encodes the iron sulfur protein of MCIII, inhibits escape from 1% O2 without affecting responses to 21% O2. Both genetic and pharmacological up-regulation of mitochondrial ROS increase cGMP levels, which contribute to the reduced hypoxia responses. Our results implicate ROS and precise regulation of intracellular cGMP in the modulation of acute responses to hypoxia by C. elegans.","lang":"eng"}],"publication_status":"published","status":"public","date_published":"2022-06-21T00:00:00Z","month":"06","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","pmid":1,"doi":"10.1371/journal.pbio.3001684"},{"article_processing_charge":"No","acknowledgement":"We thank de Bono laboratory members for helpful comments on the article and the Mass Spec Facilities at IST Austria and Max Perutz Labs for invaluable discussions and comments on how to optimize mass spec analyses of worm samples. We are grateful to Ekaterina Lashmanova for designing the degron knock-in constructs and preparing the injection mixes for CRISPR/Cas9-mediated genome editing. All LC–MS/MS analyses were performed on instruments of the Vienna BioCenter Core Facilities instrument pool.\r\nThis work was supported by a Wellcome Investigator Award (grant no.: 209504/Z/17/Z ) to M.d.B. and an ISTplus Fellowship to M.A. (Marie Sklodowska-Curie agreement no.: 754411).","date_created":"2022-09-11T22:01:55Z","publication_identifier":{"eissn":["1083-351X"],"issn":["0021-9258"]},"acknowledged_ssus":[{"_id":"Bio"}],"department":[{"_id":"MaDe"}],"volume":298,"article_type":"original","title":"Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans","external_id":{"pmid":["35933017"],"isi":["000884241800011"]},"project":[{"name":"Molecular mechanisms of neural circuit function","_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","grant_number":"209504/A/17/Z"},{"call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"}],"publication":"Journal of Biological Chemistry","year":"2022","intvolume":"       298","publisher":"Elsevier","isi":1,"type":"journal_article","article_number":"102343","scopus_import":"1","quality_controlled":"1","has_accepted_license":"1","citation":{"ieee":"M. Artan, M. Hartl, W. Chen, and M. de Bono, “Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans,” <i>Journal of Biological Chemistry</i>, vol. 298, no. 9. Elsevier, 2022.","ista":"Artan M, Hartl M, Chen W, de Bono M. 2022. Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans. Journal of Biological Chemistry. 298(9), 102343.","mla":"Artan, Murat, et al. “Depletion of Endogenously Biotinylated Carboxylases Enhances the Sensitivity of TurboID-Mediated Proximity Labeling in Caenorhabditis Elegans.” <i>Journal of Biological Chemistry</i>, vol. 298, no. 9, 102343, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.jbc.2022.102343\">10.1016/j.jbc.2022.102343</a>.","ama":"Artan M, Hartl M, Chen W, de Bono M. Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans. <i>Journal of Biological Chemistry</i>. 2022;298(9). doi:<a href=\"https://doi.org/10.1016/j.jbc.2022.102343\">10.1016/j.jbc.2022.102343</a>","apa":"Artan, M., Hartl, M., Chen, W., &#38; de Bono, M. (2022). Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans. <i>Journal of Biological Chemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jbc.2022.102343\">https://doi.org/10.1016/j.jbc.2022.102343</a>","short":"M. Artan, M. Hartl, W. Chen, M. de Bono, Journal of Biological Chemistry 298 (2022).","chicago":"Artan, Murat, Markus Hartl, Weiqiang Chen, and Mario de Bono. “Depletion of Endogenously Biotinylated Carboxylases Enhances the Sensitivity of TurboID-Mediated Proximity Labeling in Caenorhabditis Elegans.” <i>Journal of Biological Chemistry</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.jbc.2022.102343\">https://doi.org/10.1016/j.jbc.2022.102343</a>."},"_id":"12082","file_date_updated":"2022-09-12T08:14:50Z","author":[{"orcid":"0000-0001-8945-6992","last_name":"Artan","id":"C407B586-6052-11E9-B3AE-7006E6697425","first_name":"Murat","full_name":"Artan, Murat"},{"last_name":"Hartl","first_name":"Markus","full_name":"Hartl, Markus"},{"full_name":"Chen, Weiqiang","first_name":"Weiqiang","last_name":"Chen"},{"first_name":"Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"De Bono","full_name":"De Bono, Mario"}],"month":"09","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","pmid":1,"doi":"10.1016/j.jbc.2022.102343","ddc":["570"],"abstract":[{"text":"Proximity-dependent protein labeling provides a powerful in vivo strategy to characterize the interactomes of specific proteins. We previously optimized a proximity labeling protocol for Caenorhabditis elegans using the highly active biotin ligase TurboID. A significant constraint on the sensitivity of TurboID is the presence of abundant endogenously biotinylated proteins that take up bandwidth in the mass spectrometer, notably carboxylases that use biotin as a cofactor. In C. elegans, these comprise POD-2/acetyl-CoA carboxylase alpha, PCCA-1/propionyl-CoA carboxylase alpha, PYC-1/pyruvate carboxylase, and MCCC-1/methylcrotonyl-CoA carboxylase alpha. Here, we developed ways to remove these carboxylases prior to streptavidin purification and mass spectrometry by engineering their corresponding genes to add a C-terminal His10 tag. This allows us to deplete them from C. elegans lysates using immobilized metal affinity chromatography. To demonstrate the method's efficacy, we use it to expand the interactome map of the presynaptic active zone protein ELKS-1. We identify many known active zone proteins, including UNC-10/RIM, SYD-2/liprin-alpha, SAD-1/BRSK1, CLA-1/CLArinet, C16E9.2/Sentryn, as well as previously uncharacterized potentially synaptic proteins such as the ortholog of human angiomotin, F59C12.3 and the uncharacterized protein R148.3. Our approach provides a quick and inexpensive solution to a common contaminant problem in biotin-dependent proximity labeling. The approach may be applicable to other model organisms and will enable deeper and more complete analysis of interactors for proteins of interest.","lang":"eng"}],"publication_status":"published","date_published":"2022-09-01T00:00:00Z","status":"public","day":"01","corr_author":"1","file":[{"success":1,"file_name":"2022_JBC_Artan.pdf","file_size":2101656,"date_created":"2022-09-12T08:14:50Z","file_id":"12092","checksum":"e726c7b9315230e6710e0b1f1d1677e9","content_type":"application/pdf","relation":"main_file","date_updated":"2022-09-12T08:14:50Z","creator":"dernst","access_level":"open_access"}],"oa_version":"Published Version","date_updated":"2025-04-14T07:44:00Z","issue":"9","ec_funded":1,"language":[{"iso":"eng"}],"oa":1},{"volume":23,"title":"Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning","article_type":"original","external_id":{"isi":["000797302700001"],"pmid":["35586945"]},"publication":"EMBO Reports","year":"2022","department":[{"_id":"MaDe"}],"article_processing_charge":"No","acknowledgement":"We thank Scott Garforth, Sarah Garrett, Peri Kurshan, Yehuda Salzberg, PamelaStanley, Robert Townley, and members of the B€ulow laboratory for commentson the manuscript or helpful discussions during the course of this work. Wethank David Miller, Shohei Mitani, Kang Shen, and Iain Wilson for reagents,and Yuji Kohara for theyk11g705cDNA clone. We are grateful to MeeraTrivedi for sharing thedzIs117strain prior to publication. Some strains wereprovided by the Caenorhabditis Genome Center (funded by the NIH Office ofResearch Infrastructure Programs P40OD010440). This work was supportedby grants from the National Institute of Health (NIH): R01NS096672andR21NS111145to HEB; F31NS100370to MR; T32GM007288and F31HD066967to CADB; P30HD071593to Albert Einstein College of Medicine. We acknowl-edge support to MR by the Department of Neuroscience. NJRS was the recipi-ent of a Colciencias-Fulbright Fellowship and HEB of an Irma T. Hirschl/Monique Weill-Caulier research fellowship","date_created":"2023-01-16T10:01:44Z","publication_identifier":{"eissn":["1469-3178"],"issn":["1469-221X"]},"_id":"12275","author":[{"first_name":"Maisha","last_name":"Rahman","full_name":"Rahman, Maisha"},{"full_name":"Ramirez, Nelson","first_name":"Nelson","last_name":"Ramirez","id":"39831956-E4FE-11E9-85DE-0DC7E5697425"},{"last_name":"Diaz‐Balzac","first_name":"Carlos A","full_name":"Diaz‐Balzac, Carlos A"},{"full_name":"Bülow, Hannes E","first_name":"Hannes E","last_name":"Bülow"}],"quality_controlled":"1","article_number":"e54163","scopus_import":"1","has_accepted_license":"1","keyword":["Genetics","Molecular Biology","Biochemistry"],"citation":{"chicago":"Rahman, Maisha, Nelson Ramirez, Carlos A Diaz‐Balzac, and Hannes E Bülow. “Specific N-Glycans Regulate an Extracellular Adhesion Complex during Somatosensory Dendrite Patterning.” <i>EMBO Reports</i>. Embo Press, 2022. <a href=\"https://doi.org/10.15252/embr.202154163\">https://doi.org/10.15252/embr.202154163</a>.","short":"M. Rahman, N. Ramirez, C.A. Diaz‐Balzac, H.E. Bülow, EMBO Reports 23 (2022).","apa":"Rahman, M., Ramirez, N., Diaz‐Balzac, C. A., &#38; Bülow, H. E. (2022). Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning. <i>EMBO Reports</i>. Embo Press. <a href=\"https://doi.org/10.15252/embr.202154163\">https://doi.org/10.15252/embr.202154163</a>","ama":"Rahman M, Ramirez N, Diaz‐Balzac CA, Bülow HE. Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning. <i>EMBO Reports</i>. 2022;23(7). doi:<a href=\"https://doi.org/10.15252/embr.202154163\">10.15252/embr.202154163</a>","ista":"Rahman M, Ramirez N, Diaz‐Balzac CA, Bülow HE. 2022. Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning. EMBO Reports. 23(7), e54163.","mla":"Rahman, Maisha, et al. “Specific N-Glycans Regulate an Extracellular Adhesion Complex during Somatosensory Dendrite Patterning.” <i>EMBO Reports</i>, vol. 23, no. 7, e54163, Embo Press, 2022, doi:<a href=\"https://doi.org/10.15252/embr.202154163\">10.15252/embr.202154163</a>.","ieee":"M. Rahman, N. Ramirez, C. A. Diaz‐Balzac, and H. E. Bülow, “Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning,” <i>EMBO Reports</i>, vol. 23, no. 7. Embo Press, 2022."},"isi":1,"type":"journal_article","intvolume":"        23","publisher":"Embo Press","date_published":"2022-07-05T00:00:00Z","status":"public","publication_status":"published","abstract":[{"lang":"eng","text":"N-glycans are molecularly diverse sugars borne by over 70% of proteins transiting the secretory pathway and have been implicated in protein folding, stability, and localization. Mutations in genes important for N-glycosylation result in congenital disorders of glycosylation that are often associated with intellectual disability. Here, we show that structurally distinct N-glycans regulate an extracellular protein complex involved in the patterning of somatosensory dendrites in Caenorhabditis elegans. Specifically, aman-2/Golgi alpha-mannosidase II, a conserved key enzyme in the biosynthesis of specific N-glycans, regulates the activity of the Menorin adhesion complex without obviously affecting the protein stability and localization of its components. AMAN-2 functions cell-autonomously to allow for decoration of the neuronal transmembrane receptor DMA-1/LRR-TM with the correct set of high-mannose/hybrid/paucimannose N-glycans. Moreover, distinct types of N-glycans on specific N-glycosylation sites regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular proteins, forms the Menorin adhesion complex. In summary, specific N-glycan structures regulate dendrite patterning by coordinating the activity of an extracellular adhesion complex, suggesting that the molecular diversity of N-glycans can contribute to developmental specificity in the nervous system."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"doi":"10.15252/embr.202154163","month":"07","main_file_link":[{"open_access":"1","url":"https://doi.org/10.15252/embr.202154163"}],"oa":1,"language":[{"iso":"eng"}],"oa_version":"Published Version","date_updated":"2023-10-03T11:25:54Z","issue":"7","day":"05"},{"page":"1208-1210","title":"MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications","article_type":"original","external_id":{"pmid":["35188063"],"isi":["000758859600001"]},"volume":18,"year":"2022","publication":"Autophagy","acknowledgement":"This work is funded by National Research Foundation of Korea (NRF) grants NRF-2019R1A3B2067745 from the Korean Government (Ministry of Science and Information and Communications Technology (S-J.V.L.). NRF-2017R1A5A1015366 (S.Y.P, S-J.V.L). Korea Institute of Science and Technology (KIST) intramural grant (C.L).","date_created":"2022-03-13T23:01:47Z","article_processing_charge":"No","publication_identifier":{"eissn":["1554-8635"],"issn":["1554-8627"]},"department":[{"_id":"MaDe"}],"quality_controlled":"1","scopus_import":"1","citation":{"ama":"Artan M, Sohn J, Lee C, Park SY, Lee SJV. MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications. <i>Autophagy</i>. 2022;18(5):1208-1210. doi:<a href=\"https://doi.org/10.1080/15548627.2022.2039523\">10.1080/15548627.2022.2039523</a>","ista":"Artan M, Sohn J, Lee C, Park SY, Lee SJV. 2022. MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications. Autophagy. 18(5), 1208–1210.","mla":"Artan, Murat, et al. “MON-2, a Golgi Protein, Promotes Longevity by Upregulating Autophagy through Mediating Inter-Organelle Communications.” <i>Autophagy</i>, vol. 18, no. 5, Taylor &#38; Francis, 2022, pp. 1208–10, doi:<a href=\"https://doi.org/10.1080/15548627.2022.2039523\">10.1080/15548627.2022.2039523</a>.","ieee":"M. Artan, J. Sohn, C. Lee, S. Y. Park, and S. J. V. Lee, “MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications,” <i>Autophagy</i>, vol. 18, no. 5. Taylor &#38; Francis, pp. 1208–1210, 2022.","chicago":"Artan, Murat, Jooyeon Sohn, Cheolju Lee, Seung Yeol Park, and Seung Jae V. Lee. “MON-2, a Golgi Protein, Promotes Longevity by Upregulating Autophagy through Mediating Inter-Organelle Communications.” <i>Autophagy</i>. Taylor &#38; Francis, 2022. <a href=\"https://doi.org/10.1080/15548627.2022.2039523\">https://doi.org/10.1080/15548627.2022.2039523</a>.","short":"M. Artan, J. Sohn, C. Lee, S.Y. Park, S.J.V. Lee, Autophagy 18 (2022) 1208–1210.","apa":"Artan, M., Sohn, J., Lee, C., Park, S. Y., &#38; Lee, S. J. V. (2022). MON-2, a Golgi protein, promotes longevity by upregulating autophagy through mediating inter-organelle communications. <i>Autophagy</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.1080/15548627.2022.2039523\">https://doi.org/10.1080/15548627.2022.2039523</a>"},"author":[{"full_name":"Artan, Murat","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan","orcid":"0000-0001-8945-6992","first_name":"Murat"},{"full_name":"Sohn, Jooyeon","first_name":"Jooyeon","last_name":"Sohn"},{"full_name":"Lee, Cheolju","last_name":"Lee","first_name":"Cheolju"},{"full_name":"Park, Seung Yeol","last_name":"Park","first_name":"Seung Yeol"},{"first_name":"Seung Jae V.","last_name":"Lee","full_name":"Lee, Seung Jae V."}],"_id":"10846","intvolume":"        18","publisher":"Taylor & Francis","type":"journal_article","isi":1,"publication_status":"published","abstract":[{"text":"The Golgi apparatus regulates the process of modification and subcellular localization of macromolecules, including proteins and lipids. Aberrant protein sorting caused by defects in the Golgi leads to various diseases in mammals. However, the role of the Golgi apparatus in organismal longevity remained largely unknown. By employing a quantitative proteomic approach, we demonstrated that MON-2, an evolutionarily conserved Arf-GEF protein implicated in Golgi-to-endosome trafficking, promotes longevity via upregulating macroautophagy/autophagy in C. elegans. Our data using cultured mammalian cells indicate that MON2 translocates from the Golgi to the endosome under starvation conditions, subsequently increasing autophagic flux by binding LGG-1/GABARAPL2. Thus, Golgi-to-endosome trafficking appears to be an evolutionarily conserved process for the upregulation of autophagy, which contributes to organismal longevity.","lang":"eng"}],"date_published":"2022-02-19T00:00:00Z","status":"public","month":"02","main_file_link":[{"url":"https://doi.org/10.1080/15548627.2022.2039523","open_access":"1"}],"pmid":1,"doi":"10.1080/15548627.2022.2039523","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"5","oa_version":"Published Version","date_updated":"2023-10-03T10:54:54Z","language":[{"iso":"eng"}],"oa":1,"day":"19"},{"department":[{"_id":"MaDe"}],"article_processing_charge":"No","date_created":"2022-03-06T23:01:52Z","acknowledgement":"We would like to thank Gemma Chandratillake and Merav Cohen for identifying mutants and José David Moñino Sánchez for his help on neurosecretion assays. We are grateful to Kaveh Ashrafi (UCSF), Piali Sengupta (Brandeis), and the Caenorhabditis Genetic Center (funded by National Institutes of Health Infrastructure Program P40 OD010440) for strains and reagents ... and Rebecca Butcher (Univ. Florida) for C9 pheromone. We thank Tim Stevens, Paula Freire-Pritchett, Alastair Crisp, GurpreetGhattaoraya, and Fabian Amman for help with bioinformatic analysis, Ekaterina Lashmanova for help with injections, Iris Hardege for strains, and Isabel Beets (KU Leuven) and members of the de Bono Lab for comments on the manuscript. We thank the CRUK Cambridge Research Institute Genomics Core for next generation sequencing and the Flow Cytometry Facility at LMB for FACS. This research was supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF) and Scientific Computing (SciCo-p– Bioinformatics).\r\nThis work was supported by the Medical Research Council UK (Studentship to GV), an\r\nAdvanced ERC grant (269,058 ACMO to MdB), and a Wellcome Investigator Award (209504/Z/17/Z to MdB).","publication_identifier":{"eissn":["2050-084X"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"volume":11,"title":"Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans","external_id":{"isi":["000763432300001"],"pmid":["35201977"]},"article_type":"original","project":[{"name":"Molecular mechanisms of neural circuit function","_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","grant_number":"209504/A/17/Z"}],"publication":"eLife","year":"2022","isi":1,"type":"journal_article","intvolume":"        11","publisher":"eLife Sciences Publications","_id":"10826","file_date_updated":"2022-03-07T07:39:25Z","author":[{"first_name":"Giulio","last_name":"Valperga","id":"67F289DE-0D8F-11EA-9BDD-54AE3DDC885E","orcid":"0000-0001-6726-3890","full_name":"Valperga, Giulio"},{"full_name":"De Bono, Mario","first_name":"Mario","orcid":"0000-0001-8347-0443","last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87"}],"quality_controlled":"1","scopus_import":"1","article_number":"e68040","has_accepted_license":"1","citation":{"ama":"Valperga G, de Bono M. Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans. <i>eLife</i>. 2022;11. doi:<a href=\"https://doi.org/10.7554/eLife.68040\">10.7554/eLife.68040</a>","mla":"Valperga, Giulio, and Mario de Bono. “Impairing One Sensory Modality Enhances Another by Reconfiguring Peptidergic Signalling in Caenorhabditis Elegans.” <i>ELife</i>, vol. 11, e68040, eLife Sciences Publications, 2022, doi:<a href=\"https://doi.org/10.7554/eLife.68040\">10.7554/eLife.68040</a>.","ista":"Valperga G, de Bono M. 2022. Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans. eLife. 11, e68040.","ieee":"G. Valperga and M. de Bono, “Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans,” <i>eLife</i>, vol. 11. eLife Sciences Publications, 2022.","chicago":"Valperga, Giulio, and Mario de Bono. “Impairing One Sensory Modality Enhances Another by Reconfiguring Peptidergic Signalling in Caenorhabditis Elegans.” <i>ELife</i>. eLife Sciences Publications, 2022. <a href=\"https://doi.org/10.7554/eLife.68040\">https://doi.org/10.7554/eLife.68040</a>.","apa":"Valperga, G., &#38; de Bono, M. (2022). Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.68040\">https://doi.org/10.7554/eLife.68040</a>","short":"G. Valperga, M. de Bono, ELife 11 (2022)."},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","doi":"10.7554/eLife.68040","pmid":1,"month":"02","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_published":"2022-02-24T00:00:00Z","status":"public","ddc":["570"],"abstract":[{"lang":"eng","text":"Animals that lose one sensory modality often show augmented responses to other sensory inputs. The mechanisms underpinning this cross-modal plasticity are poorly understood. We probe such mechanisms by performing a forward genetic screen for mutants with enhanced O2 perception in Caenorhabditis elegans. Multiple mutants exhibiting increased O2 responsiveness concomitantly show defects in other sensory responses. One mutant, qui-1, defective in a conserved NACHT/WD40 protein, abolishes pheromone-evoked Ca2+ responses in the ADL pheromone-sensing neurons. At the same time, ADL responsiveness to pre-synaptic input from O2-sensing neurons is heightened in qui-1, and other sensory defective mutants, resulting in enhanced neurosecretion although not increased Ca2+ responses. Expressing qui-1 selectively in ADL rescues both the qui-1 ADL neurosecretory phenotype and enhanced escape from 21% O2. Profiling ADL neurons in qui-1 mutants highlights extensive changes in gene expression, notably of many neuropeptide receptors. We show that elevated ADL expression of the conserved neuropeptide receptor NPR-22 is necessary for enhanced ADL neurosecretion in qui-1 mutants, and is sufficient to confer increased ADL neurosecretion in control animals. Sensory loss can thus confer cross-modal plasticity by changing the peptidergic connectome."}],"publication_status":"published","corr_author":"1","file":[{"checksum":"cc1b9bf866d0f61f965556e0dd03d3ac","file_id":"10830","date_created":"2022-03-07T07:39:25Z","file_name":"2022_eLife_Valperga.pdf","file_size":4095591,"success":1,"access_level":"open_access","creator":"dernst","date_updated":"2022-03-07T07:39:25Z","relation":"main_file","content_type":"application/pdf"}],"day":"24","oa":1,"language":[{"iso":"eng"}],"oa_version":"Published Version","date_updated":"2026-04-02T12:45:39Z"},{"related_material":{"record":[{"relation":"used_in_publication","status":"public","id":"10322"}]},"ddc":["570"],"abstract":[{"text":"To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell-autonomous. We have discovered that, in Caenorhabditis elegans, neuronal Heat shock Factor 1 (HSF-1), the conserved master regulator of the heat shock response (HSR)- causes extensive fat remodelling in peripheral tissues. These changes include a decrease in fat desaturase and acid lipase expression in the intestine, and a global shift in the saturation levels of plasma membrane’s phospholipids. The observed remodelling of plasma membrane is in line with ectothermic adaptive responses and gives worms a cumulative advantage to warm temperatures. We have determined that at least six TAX-2/TAX-4 cGMP gated channel expressing sensory neurons and TGF-β/BMP are required for signalling across tissues to modulate fat desaturation. We also find neuronal hsf-1  is not only sufficient but also partially necessary to control the fat remodelling response and for survival at warm temperatures. This is the first study to show that a thermostat-based mechanism can cell non-autonomously coordinate membrane saturation and composition across tissues in a multicellular animal.","lang":"eng"}],"year":"2021","status":"public","date_published":"2021-12-25T00:00:00Z","title":"Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans","main_file_link":[{"url":"https://doi.org/10.5281/zenodo.5547464","open_access":"1"}],"month":"12","article_processing_charge":"No","date_created":"2023-05-23T16:40:56Z","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MaDe"}],"doi":"10.5281/ZENODO.5519410","citation":{"ieee":"L. Chauve <i>et al.</i>, “Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans.” Zenodo, 2021.","mla":"Chauve, Laetitia, et al. <i>Neuronal HSF-1 Coordinates the Propagation of Fat Desaturation across Tissues to Enable Adaptation to High Temperatures in C. Elegans</i>. Zenodo, 2021, doi:<a href=\"https://doi.org/10.5281/ZENODO.5519410\">10.5281/ZENODO.5519410</a>.","ista":"Chauve L, Hodge F, Murdoch S, Masoudzadeh F, Mann H-J, Lopez-Clavijo A, Okkenhaug H, West G, Sousa BC, Segonds-Pichon A, Li C, Wingett S, Kienberger H, Kleigrewe K, de Bono M, Wakelam M, Casanueva O. 2021. Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans, Zenodo, <a href=\"https://doi.org/10.5281/ZENODO.5519410\">10.5281/ZENODO.5519410</a>.","ama":"Chauve L, Hodge F, Murdoch S, et al. Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans. 2021. doi:<a href=\"https://doi.org/10.5281/ZENODO.5519410\">10.5281/ZENODO.5519410</a>","short":"L. Chauve, F. Hodge, S. Murdoch, F. Masoudzadeh, H.-J. Mann, A. Lopez-Clavijo, H. Okkenhaug, G. West, B.C. Sousa, A. Segonds-Pichon, C. Li, S. Wingett, H. Kienberger, K. Kleigrewe, M. de Bono, M. Wakelam, O. Casanueva, (2021).","apa":"Chauve, L., Hodge, F., Murdoch, S., Masoudzadeh, F., Mann, H.-J., Lopez-Clavijo, A., … Casanueva, O. (2021). Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans. Zenodo. <a href=\"https://doi.org/10.5281/ZENODO.5519410\">https://doi.org/10.5281/ZENODO.5519410</a>","chicago":"Chauve, Laetitia, Francesca Hodge, Sharlene Murdoch, Fatemah Masoudzadeh, Harry-Jack Mann, Andrea Lopez-Clavijo, Hanneke Okkenhaug, et al. “Neuronal HSF-1 Coordinates the Propagation of Fat Desaturation across Tissues to Enable Adaptation to High Temperatures in C. Elegans.” Zenodo, 2021. <a href=\"https://doi.org/10.5281/ZENODO.5519410\">https://doi.org/10.5281/ZENODO.5519410</a>."},"oa_version":"Published Version","date_updated":"2023-08-14T11:53:26Z","oa":1,"_id":"13069","author":[{"first_name":"Laetitia","last_name":"Chauve","full_name":"Chauve, Laetitia"},{"first_name":"Francesca","last_name":"Hodge","full_name":"Hodge, Francesca"},{"last_name":"Murdoch","first_name":"Sharlene","full_name":"Murdoch, Sharlene"},{"full_name":"Masoudzadeh, Fatemah","last_name":"Masoudzadeh","first_name":"Fatemah"},{"last_name":"Mann","first_name":"Harry-Jack","full_name":"Mann, Harry-Jack"},{"full_name":"Lopez-Clavijo, Andrea","first_name":"Andrea","last_name":"Lopez-Clavijo"},{"first_name":"Hanneke","last_name":"Okkenhaug","full_name":"Okkenhaug, Hanneke"},{"full_name":"West, Greg","first_name":"Greg","last_name":"West"},{"full_name":"Sousa, Bebiana C.","last_name":"Sousa","first_name":"Bebiana C."},{"full_name":"Segonds-Pichon, Anne","first_name":"Anne","last_name":"Segonds-Pichon"},{"first_name":"Cheryl","last_name":"Li","full_name":"Li, Cheryl"},{"last_name":"Wingett","first_name":"Steven","full_name":"Wingett, Steven"},{"full_name":"Kienberger, Hermine","last_name":"Kienberger","first_name":"Hermine"},{"last_name":"Kleigrewe","first_name":"Karin","full_name":"Kleigrewe, Karin"},{"first_name":"Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"de Bono","full_name":"de Bono, Mario"},{"full_name":"Wakelam, Michael","first_name":"Michael","last_name":"Wakelam"},{"first_name":"Olivia","last_name":"Casanueva","full_name":"Casanueva, Olivia"}],"publisher":"Zenodo","day":"25","type":"research_data_reference"},{"language":[{"iso":"eng"}],"oa":1,"issue":"7","oa_version":"Published Version","date_updated":"2024-04-10T08:57:16Z","file":[{"creator":"dernst","access_level":"open_access","date_updated":"2024-04-10T08:53:43Z","content_type":"application/pdf","relation":"main_file","checksum":"7352b195e4db6d404f702fe6ad8b55ad","date_created":"2024-04-10T08:53:43Z","file_id":"15308","success":1,"file_size":4224934,"file_name":"2021_PlosGenetics_Tang.pdf"}],"day":"01","date_published":"2021-07-01T00:00:00Z","status":"public","abstract":[{"lang":"eng","text":"The assembly of neuronal circuits involves the migrations of neurons from their place of birth to their final location in the nervous system, as well as the coordinated growth and patterning of axons and dendrites. In screens for genes required for patterning of the nervous system, we identified the <jats:italic>catp-8/P5A-ATPase</jats:italic> as an important regulator of neural patterning. P5A-ATPases are part of the P-type ATPases, a family of proteins known to serve a conserved function as transporters of ions, lipids and polyamines in unicellular eukaryotes, plants, and humans. While the function of many P-type ATPases is relatively well understood, the function of P5A-ATPases in metazoans remained elusive. We show here, that the <jats:italic>Caenorhabditis elegans</jats:italic> ortholog <jats:italic>catp-8/P5A-ATPase</jats:italic> is required for defined aspects of nervous system development. Specifically, the <jats:italic>catp-8/P5A-ATPase</jats:italic> serves functions in shaping the elaborately sculpted dendritic trees of somatosensory PVD neurons. Moreover, <jats:italic>catp-8/P5A-ATPase</jats:italic> is required for axonal guidance and repulsion at the midline, as well as embryonic and postembryonic neuronal migrations. Interestingly, not all axons at the midline require <jats:italic>catp-8/P5A-ATPase</jats:italic>, although the axons run in the same fascicles and navigate the same space. Similarly, not all neuronal migrations require <jats:italic>catp-8/P5A-ATPase</jats:italic>. A CATP-8/P5A-ATPase reporter is localized to the ER in most, if not all, tissues and <jats:italic>catp-8/P5A-ATPase</jats:italic> can function both cell-autonomously and non-autonomously to regulate neuronal development. Genetic analyses establish that <jats:italic>catp-8/P5A-ATPase</jats:italic> can function in multiple pathways, including the Menorin pathway, previously shown to control dendritic patterning in PVD, and Wnt signaling, which functions to control neuronal migrations. Lastly, we show that <jats:italic>catp-8/P5A-ATPase</jats:italic> is required for localizing select transmembrane proteins necessary for dendrite morphogenesis. Collectively, our studies suggest that <jats:italic>catp-8/P5A-ATPase</jats:italic> serves diverse, yet specific, roles in different genetic pathways and may be involved in the regulation or localization of transmembrane and secreted proteins to specific subcellular compartments."}],"publication_status":"published","ddc":["570"],"doi":"10.1371/journal.pgen.1009475","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"07","author":[{"first_name":"Leo T. H.","last_name":"Tang","full_name":"Tang, Leo T. H."},{"last_name":"Trivedi","first_name":"Meera","full_name":"Trivedi, Meera"},{"last_name":"Freund","first_name":"Jenna","full_name":"Freund, Jenna"},{"first_name":"Christopher J.","last_name":"Salazar","full_name":"Salazar, Christopher J."},{"full_name":"Rahman, Maisha","first_name":"Maisha","last_name":"Rahman"},{"first_name":"Nelson","id":"39831956-E4FE-11E9-85DE-0DC7E5697425","last_name":"Ramirez","full_name":"Ramirez, Nelson"},{"last_name":"Lee","first_name":"Garrett","full_name":"Lee, Garrett"},{"full_name":"Wang, Yu","last_name":"Wang","first_name":"Yu"},{"full_name":"Grant, Barth D.","first_name":"Barth D.","last_name":"Grant"},{"last_name":"Bülow","first_name":"Hannes E.","full_name":"Bülow, Hannes E."}],"_id":"15272","file_date_updated":"2024-04-10T08:53:43Z","has_accepted_license":"1","quality_controlled":"1","article_number":"e1009475","citation":{"apa":"Tang, L. T. H., Trivedi, M., Freund, J., Salazar, C. J., Rahman, M., Ramirez, N., … Bülow, H. E. (2021). The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning. <i>PLOS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1009475\">https://doi.org/10.1371/journal.pgen.1009475</a>","short":"L.T.H. Tang, M. Trivedi, J. Freund, C.J. Salazar, M. Rahman, N. Ramirez, G. Lee, Y. Wang, B.D. Grant, H.E. Bülow, PLOS Genetics 17 (2021).","chicago":"Tang, Leo T. H., Meera Trivedi, Jenna Freund, Christopher J. Salazar, Maisha Rahman, Nelson Ramirez, Garrett Lee, Yu Wang, Barth D. Grant, and Hannes E. Bülow. “The CATP-8/P5A-Type ATPase Functions in Multiple Pathways during Neuronal Patterning.” <i>PLOS Genetics</i>. Public Library of Science, 2021. <a href=\"https://doi.org/10.1371/journal.pgen.1009475\">https://doi.org/10.1371/journal.pgen.1009475</a>.","ieee":"L. T. H. Tang <i>et al.</i>, “The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning,” <i>PLOS Genetics</i>, vol. 17, no. 7. Public Library of Science, 2021.","ista":"Tang LTH, Trivedi M, Freund J, Salazar CJ, Rahman M, Ramirez N, Lee G, Wang Y, Grant BD, Bülow HE. 2021. The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning. PLOS Genetics. 17(7), e1009475.","mla":"Tang, Leo T. H., et al. “The CATP-8/P5A-Type ATPase Functions in Multiple Pathways during Neuronal Patterning.” <i>PLOS Genetics</i>, vol. 17, no. 7, e1009475, Public Library of Science, 2021, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1009475\">10.1371/journal.pgen.1009475</a>.","ama":"Tang LTH, Trivedi M, Freund J, et al. The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning. <i>PLOS Genetics</i>. 2021;17(7). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1009475\">10.1371/journal.pgen.1009475</a>"},"keyword":["Cancer Research","Genetics (clinical)","Genetics","Molecular Biology","Ecology","Evolution","Behavior and Systematics"],"type":"journal_article","intvolume":"        17","publisher":"Public Library of Science","external_id":{"pmid":["34197450"]},"article_type":"original","title":"The CATP-8/P5A-type ATPase functions in multiple pathways during neuronal patterning","volume":17,"year":"2021","publication":"PLOS Genetics","department":[{"_id":"MaDe"}],"date_created":"2024-04-03T07:57:12Z","article_processing_charge":"No","publication_identifier":{"issn":["1553-7404"]}},{"date_updated":"2025-04-14T07:43:46Z","oa_version":"Published Version","language":[{"iso":"eng"}],"ec_funded":1,"oa":1,"day":"17","file":[{"relation":"main_file","content_type":"application/pdf","date_updated":"2021-10-11T14:15:07Z","access_level":"open_access","creator":"cchlebak","file_size":1774624,"file_name":"2021_eLife_VuongBrender.pdf","success":1,"file_id":"10122","date_created":"2021-10-11T14:15:07Z","checksum":"b465e172d2b1f57aa26a2571a085d052"}],"abstract":[{"lang":"eng","text":"The ubiquitous Ca2+ sensor calmodulin (CaM) binds and regulates many proteins, including ion channels, CaM kinases, and calcineurin, according to Ca2+-CaM levels. What regulates neuronal CaM levels, is, however, unclear. CaM-binding transcription activators (CAMTAs) are ancient proteins expressed broadly in nervous systems and whose loss confers pleiotropic behavioral defects in flies, mice, and humans. Using Caenorhabditis elegans and Drosophila, we show that CAMTAs control neuronal CaM levels. The behavioral and neuronal Ca2+ signaling defects in mutants lacking camt-1, the sole C. elegans CAMTA, can be rescued by supplementing neuronal CaM. CAMT-1 binds multiple sites in the CaM promoter and deleting these sites phenocopies camt-1. Our data suggest CAMTAs mediate a conserved and general mechanism that controls neuronal CaM levels, thereby regulating Ca2+ signaling, physiology, and behavior."}],"publication_status":"published","ddc":["610"],"date_published":"2021-09-17T00:00:00Z","status":"public","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"09","pmid":1,"doi":"10.7554/eLife.68238","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","has_accepted_license":"1","quality_controlled":"1","scopus_import":"1","article_number":"e68238","citation":{"mla":"Vuong-Brender, Thanh, et al. “Neuronal Calmodulin Levels Are Controlled by CAMTA Transcription Factors.” <i>ELife</i>, vol. 10, e68238, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/eLife.68238\">10.7554/eLife.68238</a>.","ieee":"T. Vuong-Brender, S. Flynn, Y. Vallis, and M. de Bono, “Neuronal calmodulin levels are controlled by CAMTA transcription factors,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","ista":"Vuong-Brender T, Flynn S, Vallis Y, de Bono M. 2021. Neuronal calmodulin levels are controlled by CAMTA transcription factors. eLife. 10, e68238.","ama":"Vuong-Brender T, Flynn S, Vallis Y, de Bono M. Neuronal calmodulin levels are controlled by CAMTA transcription factors. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/eLife.68238\">10.7554/eLife.68238</a>","apa":"Vuong-Brender, T., Flynn, S., Vallis, Y., &#38; de Bono, M. (2021). Neuronal calmodulin levels are controlled by CAMTA transcription factors. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.68238\">https://doi.org/10.7554/eLife.68238</a>","short":"T. Vuong-Brender, S. Flynn, Y. Vallis, M. de Bono, ELife 10 (2021).","chicago":"Vuong-Brender, Thanh, Sean Flynn, Yvonne Vallis, and Mario de Bono. “Neuronal Calmodulin Levels Are Controlled by CAMTA Transcription Factors.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/eLife.68238\">https://doi.org/10.7554/eLife.68238</a>."},"author":[{"last_name":"Vuong-Brender","id":"D389312E-10C4-11EA-ABF4-A4B43DDC885E","first_name":"Thanh","full_name":"Vuong-Brender, Thanh"},{"full_name":"Flynn, Sean","first_name":"Sean","last_name":"Flynn"},{"first_name":"Yvonne","id":"05A2795C-31B5-11EA-83A7-7DA23DDC885E","last_name":"Vallis","full_name":"Vallis, Yvonne"},{"full_name":"De Bono, Mario","first_name":"Mario","orcid":"0000-0001-8347-0443","last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87"}],"_id":"10116","file_date_updated":"2021-10-11T14:15:07Z","intvolume":"        10","publisher":"eLife Sciences Publications","type":"journal_article","isi":1,"title":"Neuronal calmodulin levels are controlled by CAMTA transcription factors","article_type":"original","external_id":{"isi":["000695716100001"],"pmid":["34499028"]},"volume":10,"year":"2021","project":[{"name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"754411"}],"publication":"eLife","acknowledgement":"The authors thank the MRC-LMB Flow Cytometry facility and Imaging Service for support, the Cancer Research UK Cambridge Institute Genomics Core for Next Generation Sequencing, Julie Ahringer and Alex Appert for advice and technical help for ChIP-seq experiments, Paula Freire-Pritchett, Tim Stevens, and Gurpreet Ghattaoraya for RNA-seq and ChIP-seq analyses, Nikos Chronis for the TN-XL plasmid, Hong-Sheng Li and Daisuke Yamamoto for generously sending the tes2 and cro mutants, Daria Siekhaus for hosting the fly work, Michaela Misova for technical assistance. The authors are very grateful to Salihah Ece Sönmez for teaching us how to dissect, mount and stain Drosophila retinae. This work was supported by an Advanced ERC grant (269058 ACMO) and a Wellcome Investigator Award (209504/Z/17/Z) to MdB, and an IST Plus Fellowship to TV-B (Marie Sklodowska-Curie Agreement no 754411).","date_created":"2021-10-10T22:01:22Z","article_processing_charge":"No","publication_identifier":{"eissn":["2050-084X"]},"department":[{"_id":"MaDe"}]},{"month":"09","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1016/J.JBC.2021.101094","ddc":["612"],"abstract":[{"text":"Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. The nematode Caenorhabditis elegans is one of the most intensely studied organisms in biology, offering many advantages for biochemistry. Using the highly active biotin ligase TurboID, we optimize here a proximity labeling protocol for C. elegans. An advantage of TurboID is that biotin's high affinity for streptavidin means biotin-labeled proteins can be affinity-purified under harsh denaturing conditions. By combining extensive sonication with aggressive denaturation using SDS and urea, we achieved near-complete solubilization of worm proteins. We then used this protocol to characterize the proteomes of the worm gut, muscle, skin, and nervous system. Neurons are among the smallest C. elegans cells. To probe the method's sensitivity, we expressed TurboID exclusively in the two AFD neurons and showed that the protocol could identify known and previously unknown proteins expressed selectively in AFD. The active zones of synapses are composed of a protein matrix that is difficult to solubilize and purify. To test if our protocol could solubilize active zone proteins, we knocked TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identified many known ELKS-1-interacting active zone proteins, as well as previously uncharacterized synaptic proteins. Versatile vectors and the inherent advantages of using C. elegans, including fast growth and the ability to rapidly make and functionally test knock-ins, make proximity labeling a valuable addition to the armory of this model organism.","lang":"eng"}],"publication_status":"published","status":"public","date_published":"2021-09-01T00:00:00Z","day":"01","file":[{"file_size":1680010,"file_name":"2021_JBC_Artan.pdf","success":1,"checksum":"19e39d36c5b9387c6dc0e89c9ae856ab","file_id":"10121","date_created":"2021-10-11T12:20:58Z","relation":"main_file","content_type":"application/pdf","access_level":"open_access","creator":"cchlebak","date_updated":"2021-10-11T12:20:58Z"}],"date_updated":"2025-04-14T07:43:46Z","oa_version":"Published Version","issue":"3","ec_funded":1,"oa":1,"language":[{"iso":"eng"}],"publication_identifier":{"issn":["0021-9258"],"eissn":["1083-351X"]},"article_processing_charge":"Yes","date_created":"2021-10-10T22:01:23Z","acknowledgement":"We thank de Bono lab members for helpful comments on the manuscript, IST Austria and University of Vienna Mass Spec Facilities for invaluable discussions and comments for the optimization of mass spec analyses of worm samples. The biotin auxotropic E. coli strain MG1655bioB:kan was gift from John Cronan (University of Illinois) and was kindly sent to us by Jessica Feldman and Ariana Sanchez (Stanford University). dg398 pEntryslot2_mNeongreen::3XFLAG::stop and dg397 pEntryslot3_mNeongreen::3XFLAG::stop::unc-54 3′UTR entry vector were kindly shared by Dr Dominique Glauser (University of Fribourg). Codon-optimized mScarlet vector was a generous gift from Dr Manuel Zimmer (University of Vienna).","department":[{"_id":"MaDe"},{"_id":"LifeSc"}],"project":[{"call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"}],"publication":"Journal of Biological Chemistry","year":"2021","volume":297,"title":"Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling","external_id":{"isi":["000706409200006"]},"article_type":"original","publisher":"Elsevier","intvolume":"       297","isi":1,"type":"journal_article","citation":{"chicago":"Artan, Murat, Stephen Barratt, Sean M. Flynn, Farida Begum, Mark Skehel, Armel Nicolas, and Mario de Bono. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>.","short":"M. Artan, S. Barratt, S.M. Flynn, F. Begum, M. Skehel, A. Nicolas, M. de Bono, Journal of Biological Chemistry 297 (2021).","apa":"Artan, M., Barratt, S., Flynn, S. M., Begum, F., Skehel, M., Nicolas, A., &#38; de Bono, M. (2021). Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>","ama":"Artan M, Barratt S, Flynn SM, et al. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. 2021;297(3). doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>","ista":"Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M. 2021. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. Journal of Biological Chemistry. 297(3), 101094.","ieee":"M. Artan <i>et al.</i>, “Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling,” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3. Elsevier, 2021.","mla":"Artan, Murat, et al. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3, 101094, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>."},"scopus_import":"1","article_number":"101094","quality_controlled":"1","has_accepted_license":"1","_id":"10117","file_date_updated":"2021-10-11T12:20:58Z","author":[{"full_name":"Artan, Murat","orcid":"0000-0001-8945-6992","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan","first_name":"Murat"},{"full_name":"Barratt, Stephen","first_name":"Stephen","last_name":"Barratt","id":"57740d2b-2a88-11ec-97cf-d9e6d1b39677"},{"first_name":"Sean M.","last_name":"Flynn","full_name":"Flynn, Sean M."},{"full_name":"Begum, Farida","first_name":"Farida","last_name":"Begum"},{"last_name":"Skehel","first_name":"Mark","full_name":"Skehel, Mark"},{"first_name":"Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas","full_name":"Nicolas, Armel"},{"first_name":"Mario","last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443","full_name":"De Bono, Mario"}]},{"publisher":"Public Library of Science","intvolume":"        19","type":"journal_article","isi":1,"citation":{"chicago":"Chauve, Laetitia, Francesca Hodge, Sharlene Murdoch, Fatemah Masoudzadeh, Harry Jack Mann, Andrea Lopez-Clavijo, Hanneke Okkenhaug, et al. “Neuronal HSF-1 Coordinates the Propagation of Fat Desaturation across Tissues to Enable Adaptation to High Temperatures in C. Elegans.” <i>PLoS Biology</i>. Public Library of Science, 2021. <a href=\"https://doi.org/10.1371/journal.pbio.3001431\">https://doi.org/10.1371/journal.pbio.3001431</a>.","short":"L. Chauve, F. Hodge, S. Murdoch, F. Masoudzadeh, H.J. Mann, A. Lopez-Clavijo, H. Okkenhaug, G. West, B.C. Sousa, A. Segonds-Pichon, C. Li, S. Wingett, H. Kienberger, K. Kleigrewe, M. de Bono, M. Wakelam, O. Casanueva, PLoS Biology 19 (2021).","apa":"Chauve, L., Hodge, F., Murdoch, S., Masoudzadeh, F., Mann, H. J., Lopez-Clavijo, A., … Casanueva, O. (2021). Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.3001431\">https://doi.org/10.1371/journal.pbio.3001431</a>","ama":"Chauve L, Hodge F, Murdoch S, et al. Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans. <i>PLoS Biology</i>. 2021;19(11). doi:<a href=\"https://doi.org/10.1371/journal.pbio.3001431\">10.1371/journal.pbio.3001431</a>","mla":"Chauve, Laetitia, et al. “Neuronal HSF-1 Coordinates the Propagation of Fat Desaturation across Tissues to Enable Adaptation to High Temperatures in C. Elegans.” <i>PLoS Biology</i>, vol. 19, no. 11, e3001431, Public Library of Science, 2021, doi:<a href=\"https://doi.org/10.1371/journal.pbio.3001431\">10.1371/journal.pbio.3001431</a>.","ieee":"L. Chauve <i>et al.</i>, “Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans,” <i>PLoS Biology</i>, vol. 19, no. 11. Public Library of Science, 2021.","ista":"Chauve L, Hodge F, Murdoch S, Masoudzadeh F, Mann HJ, Lopez-Clavijo A, Okkenhaug H, West G, Sousa BC, Segonds-Pichon A, Li C, Wingett S, Kienberger H, Kleigrewe K, de Bono M, Wakelam M, Casanueva O. 2021. Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans. PLoS Biology. 19(11), e3001431."},"has_accepted_license":"1","scopus_import":"1","article_number":"e3001431","quality_controlled":"1","author":[{"full_name":"Chauve, Laetitia","last_name":"Chauve","first_name":"Laetitia"},{"last_name":"Hodge","first_name":"Francesca","full_name":"Hodge, Francesca"},{"last_name":"Murdoch","first_name":"Sharlene","full_name":"Murdoch, Sharlene"},{"full_name":"Masoudzadeh, Fatemah","last_name":"Masoudzadeh","first_name":"Fatemah"},{"last_name":"Mann","first_name":"Harry Jack","full_name":"Mann, Harry Jack"},{"first_name":"Andrea","last_name":"Lopez-Clavijo","full_name":"Lopez-Clavijo, Andrea"},{"full_name":"Okkenhaug, Hanneke","last_name":"Okkenhaug","first_name":"Hanneke"},{"last_name":"West","first_name":"Greg","full_name":"West, Greg"},{"full_name":"Sousa, Bebiana C.","first_name":"Bebiana C.","last_name":"Sousa"},{"last_name":"Segonds-Pichon","first_name":"Anne","full_name":"Segonds-Pichon, Anne"},{"first_name":"Cheryl","last_name":"Li","full_name":"Li, Cheryl"},{"last_name":"Wingett","first_name":"Steven","full_name":"Wingett, Steven"},{"first_name":"Hermine","last_name":"Kienberger","full_name":"Kienberger, Hermine"},{"first_name":"Karin","last_name":"Kleigrewe","full_name":"Kleigrewe, Karin"},{"full_name":"De Bono, Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"De Bono","first_name":"Mario"},{"full_name":"Wakelam, Michael","first_name":"Michael","last_name":"Wakelam"},{"full_name":"Casanueva, Olivia","last_name":"Casanueva","first_name":"Olivia"}],"file_date_updated":"2021-11-22T09:34:03Z","_id":"10322","publication_identifier":{"eissn":["1545-7885"],"issn":["1544-9173"]},"acknowledgement":"We dedicate this work to the memory of Michael J.O. Wakelam. We would like to acknowledge Michael Fasseas (Invermis, Magnitude Biosciences) for plasmid injections and Sunny Biotech for transgenics; Catalina Vallejos and John Marioni for statistical advice at the beginning of the work; Simon Walker, Imaging, Bioinformatics and Lipidomics Facilities at Babraham Institute for technical support; and Cindy Voisine, Michael Witting, Jon Houseley, Len Stephens, Carmen Nussbaum Krammer, Rebeca Aldunate, Patricija van Oosten-Hawle, Jean-Louis Bessereau, and Jane Alfred for feedback on the manuscript. We thank Andy Dillin, Atsushi Kuhara, Amy Walker, Andrew Leifer, Yun Zhang, and Michalis Barkoulas for reagents and Julie Ahringer, Anne Ferguson-Smith, and Anne Corcoran for support and helpful discussions. We also acknowledge Babraham Institute Facilities.","date_created":"2021-11-21T23:01:28Z","article_processing_charge":"No","department":[{"_id":"MaDe"}],"related_material":{"record":[{"relation":"research_data","id":"13069","status":"public"}]},"year":"2021","publication":"PLoS Biology","title":"Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans","article_type":"original","external_id":{"isi":["000715818400001"],"pmid":["34723964"]},"volume":19,"day":"01","file":[{"checksum":"0c61b667f814fd9435b3ac42036fc36d","date_created":"2021-11-22T09:34:03Z","file_id":"10330","success":1,"file_name":"2021_PLoSBio_Chauve.pdf","file_size":4069215,"creator":"cchlebak","access_level":"open_access","date_updated":"2021-11-22T09:34:03Z","content_type":"application/pdf","relation":"main_file"}],"issue":"11","date_updated":"2023-08-14T11:53:27Z","oa_version":"Published Version","language":[{"iso":"eng"}],"oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"11","pmid":1,"doi":"10.1371/journal.pbio.3001431","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","abstract":[{"lang":"eng","text":"To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell autonomous. We have discovered that, in Caenorhabditis elegans, neuronal heat shock factor 1 (HSF-1), the conserved master regulator of the heat shock response (HSR), causes extensive fat remodeling in peripheral tissues. These changes include a decrease in fat desaturase and acid lipase expression in the intestine and a global shift in the saturation levels of plasma membrane’s phospholipids. The observed remodeling of plasma membrane is in line with ectothermic adaptive responses and gives worms a cumulative advantage to warm temperatures. We have determined that at least 6 TAX-2/TAX-4 cyclic guanosine monophosphate (cGMP) gated channel expressing sensory neurons, and transforming growth factor ß (TGF-β)/bone morphogenetic protein (BMP) are required for signaling across tissues to modulate fat desaturation. We also find neuronal hsf-1 is not only sufficient but also partially necessary to control the fat remodeling response and for survival at warm temperatures. This is the first study to show that a thermostat-based mechanism can cell nonautonomously coordinate membrane saturation and composition across tissues in a multicellular animal."}],"ddc":["570"],"status":"public","date_published":"2021-11-01T00:00:00Z"},{"date_published":"2020-09-20T00:00:00Z","status":"public","ddc":["570"],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"doi":"10.17912/MICROPUB.BIOLOGY.000303","month":"09","OA_type":"gold","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"language":[{"iso":"eng"}],"oa_version":"Published Version","date_updated":"2025-03-11T08:30:41Z","issue":"9","file":[{"relation":"main_file","content_type":"application/pdf","date_updated":"2025-03-11T08:27:40Z","access_level":"open_access","creator":"dernst","file_name":"2020_MicroPublBio_Kazatskaya.pdf","file_size":1486239,"success":1,"file_id":"19383","date_created":"2025-03-11T08:27:40Z","checksum":"14a7cad20775521ce85e0e3c77aa7936"}],"day":"20","volume":2020,"title":"The URX oxygen-sensing neurons in C. elegans are ciliated","article_type":"original","external_id":{"pmid":["33005885"]},"publication":"microPublication Biology","year":"2020","OA_place":"publisher","department":[{"_id":"MaDe"}],"article_processing_charge":"Yes","acknowledgement":"We thank Maureen Barr, Martin Harterink, Max Heiman and Inna Nechipurenko for reagents, the Caenorhabditis Genetics Center for strains, and the Sengupta lab for comments and advice.\r\nThis work was funded in part by the NIH (R35 GM122463 – P.S., and F32 DC018453 – A.P.), and the EMBO (ALTF 302-2019 – N.A-W.).","date_created":"2025-03-07T08:21:51Z","publication_identifier":{"eissn":["2578-9430"]},"_id":"19306","file_date_updated":"2025-03-11T08:27:40Z","author":[{"full_name":"Kazatskaya, Anna","last_name":"Kazatskaya","first_name":"Anna"},{"last_name":"Yuan","first_name":"Lisa","full_name":"Yuan, Lisa"},{"first_name":"Niko Paresh","last_name":"Amin-Wetzel","id":"E95D3014-9D8C-11E9-9C80-D2F8E5697425","full_name":"Amin-Wetzel, Niko Paresh"},{"last_name":"Philbrook","first_name":"Alison","full_name":"Philbrook, Alison"},{"first_name":"Mario","last_name":"de Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443","full_name":"de Bono, Mario"},{"full_name":"Sengupta, Piali","last_name":"Sengupta","first_name":"Piali"}],"article_number":"303","quality_controlled":"1","has_accepted_license":"1","citation":{"mla":"Kazatskaya, Anna, et al. “The URX Oxygen-Sensing Neurons in C. Elegans Are Ciliated.” <i>MicroPublication Biology</i>, vol. 2020, no. 9, 303, Caltech Library, 2020, doi:<a href=\"https://doi.org/10.17912/MICROPUB.BIOLOGY.000303\">10.17912/MICROPUB.BIOLOGY.000303</a>.","ista":"Kazatskaya A, Yuan L, Amin-Wetzel NP, Philbrook A, de Bono M, Sengupta P. 2020. The URX oxygen-sensing neurons in C. elegans are ciliated. microPublication Biology. 2020(9), 303.","ieee":"A. Kazatskaya, L. Yuan, N. P. Amin-Wetzel, A. Philbrook, M. de Bono, and P. Sengupta, “The URX oxygen-sensing neurons in C. elegans are ciliated,” <i>microPublication Biology</i>, vol. 2020, no. 9. Caltech Library, 2020.","ama":"Kazatskaya A, Yuan L, Amin-Wetzel NP, Philbrook A, de Bono M, Sengupta P. The URX oxygen-sensing neurons in C. elegans are ciliated. <i>microPublication Biology</i>. 2020;2020(9). doi:<a href=\"https://doi.org/10.17912/MICROPUB.BIOLOGY.000303\">10.17912/MICROPUB.BIOLOGY.000303</a>","short":"A. Kazatskaya, L. Yuan, N.P. Amin-Wetzel, A. Philbrook, M. de Bono, P. Sengupta, MicroPublication Biology 2020 (2020).","apa":"Kazatskaya, A., Yuan, L., Amin-Wetzel, N. P., Philbrook, A., de Bono, M., &#38; Sengupta, P. (2020). The URX oxygen-sensing neurons in C. elegans are ciliated. <i>MicroPublication Biology</i>. Caltech Library. <a href=\"https://doi.org/10.17912/MICROPUB.BIOLOGY.000303\">https://doi.org/10.17912/MICROPUB.BIOLOGY.000303</a>","chicago":"Kazatskaya, Anna, Lisa Yuan, Niko Paresh Amin-Wetzel, Alison Philbrook, Mario de Bono, and Piali Sengupta. “The URX Oxygen-Sensing Neurons in C. Elegans Are Ciliated.” <i>MicroPublication Biology</i>. Caltech Library, 2020. <a href=\"https://doi.org/10.17912/MICROPUB.BIOLOGY.000303\">https://doi.org/10.17912/MICROPUB.BIOLOGY.000303</a>."},"DOAJ_listed":"1","type":"journal_article","intvolume":"      2020","publisher":"Caltech Library"},{"publisher":"American Association for the Advancement of Science","intvolume":"         6","type":"journal_article","citation":{"chicago":"Park, Sangsoon, Murat Artan, Seung Hyun Han, Hae-Eun H. Park, Yoonji Jung, Ara B. Hwang, Won Sik Shin, Kyong-Tai Kim, and Seung-Jae V. Lee. “VRK-1 Extends Life Span by Activation of AMPK via Phosphorylation.” <i>Science Advances</i>. American Association for the Advancement of Science, 2020. <a href=\"https://doi.org/10.1126/sciadv.aaw7824\">https://doi.org/10.1126/sciadv.aaw7824</a>.","apa":"Park, S., Artan, M., Han, S. H., Park, H.-E. H., Jung, Y., Hwang, A. B., … Lee, S.-J. V. (2020). VRK-1 extends life span by activation of AMPK via phosphorylation. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aaw7824\">https://doi.org/10.1126/sciadv.aaw7824</a>","short":"S. Park, M. Artan, S.H. Han, H.-E.H. Park, Y. Jung, A.B. Hwang, W.S. Shin, K.-T. Kim, S.-J.V. Lee, Science Advances 6 (2020).","ama":"Park S, Artan M, Han SH, et al. VRK-1 extends life span by activation of AMPK via phosphorylation. <i>Science Advances</i>. 2020;6(27). doi:<a href=\"https://doi.org/10.1126/sciadv.aaw7824\">10.1126/sciadv.aaw7824</a>","ieee":"S. Park <i>et al.</i>, “VRK-1 extends life span by activation of AMPK via phosphorylation,” <i>Science Advances</i>, vol. 6, no. 27. American Association for the Advancement of Science, 2020.","mla":"Park, Sangsoon, et al. “VRK-1 Extends Life Span by Activation of AMPK via Phosphorylation.” <i>Science Advances</i>, vol. 6, no. 27, aaw7824, American Association for the Advancement of Science, 2020, doi:<a href=\"https://doi.org/10.1126/sciadv.aaw7824\">10.1126/sciadv.aaw7824</a>.","ista":"Park S, Artan M, Han SH, Park H-EH, Jung Y, Hwang AB, Shin WS, Kim K-T, Lee S-JV. 2020. VRK-1 extends life span by activation of AMPK via phosphorylation. Science Advances. 6(27), aaw7824."},"has_accepted_license":"1","article_number":"aaw7824","quality_controlled":"1","author":[{"full_name":"Park, Sangsoon","first_name":"Sangsoon","last_name":"Park"},{"last_name":"Artan","id":"C407B586-6052-11E9-B3AE-7006E6697425","orcid":"0000-0001-8945-6992","first_name":"Murat","full_name":"Artan, Murat"},{"full_name":"Han, Seung Hyun","first_name":"Seung Hyun","last_name":"Han"},{"last_name":"Park","first_name":"Hae-Eun H.","full_name":"Park, Hae-Eun H."},{"full_name":"Jung, Yoonji","first_name":"Yoonji","last_name":"Jung"},{"last_name":"Hwang","first_name":"Ara B.","full_name":"Hwang, Ara B."},{"last_name":"Shin","first_name":"Won Sik","full_name":"Shin, Won Sik"},{"first_name":"Kyong-Tai","last_name":"Kim","full_name":"Kim, Kyong-Tai"},{"last_name":"Lee","first_name":"Seung-Jae V.","full_name":"Lee, Seung-Jae V."}],"_id":"15057","file_date_updated":"2024-03-04T09:46:41Z","publication_identifier":{"eissn":["2375-2548"]},"date_created":"2024-03-04T09:41:57Z","acknowledgement":"This research was supported by grants NRF-2019R1A3B2067745 and NRF-2017R1A5A1015366 funded by the Korean Government (MSIT) through the National Research Foundation (NRF) of Korea to S.-J.V.L. and by grant Basic Science Research Program (No. 2019R1A2C2009440) funded by the Korean Government (MSIT) through the NRF of Korea to K.-T.K. ","article_processing_charge":"No","department":[{"_id":"MaDe"}],"year":"2020","publication":"Science Advances","article_type":"original","title":"VRK-1 extends life span by activation of AMPK via phosphorylation","volume":6,"day":"01","file":[{"file_id":"15058","date_created":"2024-03-04T09:46:41Z","checksum":"a37157cd0de709dce5fe03f4a31cd0b6","file_size":1864415,"file_name":"2020_ScienceAdvances_Park.pdf","success":1,"date_updated":"2024-03-04T09:46:41Z","access_level":"open_access","creator":"dernst","relation":"main_file","content_type":"application/pdf"}],"issue":"27","date_updated":"2024-03-04T09:52:09Z","oa_version":"Published Version","oa":1,"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png"},"month":"07","doi":"10.1126/sciadv.aaw7824","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Vaccinia virus–related kinase (VRK) is an evolutionarily conserved nuclear protein kinase. VRK-1, the single Caenorhabditis elegans VRK ortholog, functions in cell division and germline proliferation. However, the role of VRK-1 in postmitotic cells and adult life span remains unknown. Here, we show that VRK-1 increases organismal longevity by activating the cellular energy sensor, AMP-activated protein kinase (AMPK), via direct phosphorylation. We found that overexpression of vrk-1 in the soma of adult C. elegans increased life span and, conversely, inhibition of vrk-1 decreased life span. In addition, vrk-1 was required for longevity conferred by mutations that inhibit C. elegans mitochondrial respiration, which requires AMPK. VRK-1 directly phosphorylated and up-regulated AMPK in both C. elegans and cultured human cells. Thus, our data show that the somatic nuclear kinase, VRK-1, promotes longevity through AMPK activation, and this function appears to be conserved between C. elegans and humans."}],"publication_status":"published","ddc":["570"],"status":"public","date_published":"2020-07-01T00:00:00Z"},{"language":[{"iso":"eng"}],"oa":1,"issue":"1","oa_version":"Published Version","date_updated":"2024-10-09T20:59:20Z","file":[{"creator":"dernst","access_level":"open_access","date_updated":"2020-07-14T12:48:00Z","content_type":"application/pdf","relation":"main_file","checksum":"799bfd297a008753a688b30d3958fa48","date_created":"2020-03-02T15:43:57Z","file_id":"7558","file_size":3294066,"file_name":"2020_Neuron_Beets.pdf"}],"corr_author":"1","day":"08","status":"public","date_published":"2020-01-08T00:00:00Z","abstract":[{"text":"The extent to which behavior is shaped by experience varies between individuals. Genetic differences contribute to this variation, but the neural mechanisms are not understood. Here, we dissect natural variation in the behavioral flexibility of two Caenorhabditis elegans wild strains. In one strain, a memory of exposure to 21% O2 suppresses CO2-evoked locomotory arousal; in the other, CO2 evokes arousal regardless of previous O2 experience. We map that variation to a polymorphic dendritic scaffold protein, ARCP-1, expressed in sensory neurons. ARCP-1 binds the Ca2+-dependent phosphodiesterase PDE-1 and co-localizes PDE-1 with molecular sensors for CO2 at dendritic ends. Reducing ARCP-1 or PDE-1 activity promotes CO2 escape by altering neuropeptide expression in the BAG CO2 sensors. Variation in ARCP-1 alters behavioral plasticity in multiple paradigms. Our findings are reminiscent of genetic accommodation, an evolutionary process by which phenotypic flexibility in response to environmental variation is reset by genetic change.","lang":"eng"}],"publication_status":"published","ddc":["570"],"doi":"10.1016/j.neuron.2019.10.001","pmid":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"01","author":[{"last_name":"Beets","first_name":"Isabel","full_name":"Beets, Isabel"},{"first_name":"Gaotian","last_name":"Zhang","full_name":"Zhang, Gaotian"},{"full_name":"Fenk, Lorenz A.","first_name":"Lorenz A.","last_name":"Fenk"},{"last_name":"Chen","first_name":"Changchun","full_name":"Chen, Changchun"},{"full_name":"Nelson, Geoffrey M.","first_name":"Geoffrey M.","last_name":"Nelson"},{"last_name":"Félix","first_name":"Marie-Anne","full_name":"Félix, Marie-Anne"},{"full_name":"de Bono, Mario","orcid":"0000-0001-8347-0443","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"de Bono","first_name":"Mario"}],"_id":"7546","file_date_updated":"2020-07-14T12:48:00Z","citation":{"ama":"Beets I, Zhang G, Fenk LA, et al. Natural variation in a dendritic scaffold protein remodels experience-dependent plasticity by altering neuropeptide expression. <i>Neuron</i>. 2020;105(1):106-121.e10. doi:<a href=\"https://doi.org/10.1016/j.neuron.2019.10.001\">10.1016/j.neuron.2019.10.001</a>","mla":"Beets, Isabel, et al. “Natural Variation in a Dendritic Scaffold Protein Remodels Experience-Dependent Plasticity by Altering Neuropeptide Expression.” <i>Neuron</i>, vol. 105, no. 1, Cell Press, 2020, p. 106–121.e10, doi:<a href=\"https://doi.org/10.1016/j.neuron.2019.10.001\">10.1016/j.neuron.2019.10.001</a>.","ista":"Beets I, Zhang G, Fenk LA, Chen C, Nelson GM, Félix M-A, de Bono M. 2020. Natural variation in a dendritic scaffold protein remodels experience-dependent plasticity by altering neuropeptide expression. Neuron. 105(1), 106–121.e10.","ieee":"I. Beets <i>et al.</i>, “Natural variation in a dendritic scaffold protein remodels experience-dependent plasticity by altering neuropeptide expression,” <i>Neuron</i>, vol. 105, no. 1. Cell Press, p. 106–121.e10, 2020.","chicago":"Beets, Isabel, Gaotian Zhang, Lorenz A. Fenk, Changchun Chen, Geoffrey M. Nelson, Marie-Anne Félix, and Mario de Bono. “Natural Variation in a Dendritic Scaffold Protein Remodels Experience-Dependent Plasticity by Altering Neuropeptide Expression.” <i>Neuron</i>. Cell Press, 2020. <a href=\"https://doi.org/10.1016/j.neuron.2019.10.001\">https://doi.org/10.1016/j.neuron.2019.10.001</a>.","apa":"Beets, I., Zhang, G., Fenk, L. A., Chen, C., Nelson, G. M., Félix, M.-A., &#38; de Bono, M. (2020). Natural variation in a dendritic scaffold protein remodels experience-dependent plasticity by altering neuropeptide expression. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2019.10.001\">https://doi.org/10.1016/j.neuron.2019.10.001</a>","short":"I. Beets, G. Zhang, L.A. Fenk, C. Chen, G.M. Nelson, M.-A. Félix, M. de Bono, Neuron 105 (2020) 106–121.e10."},"has_accepted_license":"1","quality_controlled":"1","type":"journal_article","isi":1,"publisher":"Cell Press","intvolume":"       105","year":"2020","publication":"Neuron","external_id":{"pmid":["31757604"],"isi":["000507341300012"]},"title":"Natural variation in a dendritic scaffold protein remodels experience-dependent plasticity by altering neuropeptide expression","article_type":"original","volume":105,"page":"106-121.e10","department":[{"_id":"MaDe"}],"publication_identifier":{"issn":["0896-6273"]},"date_created":"2020-02-28T10:43:39Z","article_processing_charge":"No"},{"year":"2020","publication":"Nature Communications","article_type":"original","external_id":{"pmid":["32350248"],"isi":["000531855500029"]},"title":"MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity","volume":11,"department":[{"_id":"MaDe"}],"publication_identifier":{"eissn":["2041-1723"]},"date_created":"2020-05-10T22:00:47Z","article_processing_charge":"No","author":[{"first_name":"Sean M.","last_name":"Flynn","full_name":"Flynn, Sean M."},{"first_name":"Changchun","last_name":"Chen","full_name":"Chen, Changchun"},{"full_name":"Artan, Murat","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan","orcid":"0000-0001-8945-6992","first_name":"Murat"},{"last_name":"Barratt","first_name":"Stephen","full_name":"Barratt, Stephen"},{"first_name":"Alastair","last_name":"Crisp","full_name":"Crisp, Alastair"},{"full_name":"Nelson, Geoffrey M.","first_name":"Geoffrey M.","last_name":"Nelson"},{"first_name":"Sew Yeu","last_name":"Peak-Chew","full_name":"Peak-Chew, Sew Yeu"},{"first_name":"Farida","last_name":"Begum","full_name":"Begum, Farida"},{"first_name":"Mark","last_name":"Skehel","full_name":"Skehel, Mark"},{"last_name":"De Bono","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443","first_name":"Mario","full_name":"De Bono, Mario"}],"file_date_updated":"2020-07-14T12:48:03Z","_id":"7804","citation":{"apa":"Flynn, S. M., Chen, C., Artan, M., Barratt, S., Crisp, A., Nelson, G. M., … de Bono, M. (2020). MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15872-y\">https://doi.org/10.1038/s41467-020-15872-y</a>","short":"S.M. Flynn, C. Chen, M. Artan, S. Barratt, A. Crisp, G.M. Nelson, S.Y. Peak-Chew, F. Begum, M. Skehel, M. de Bono, Nature Communications 11 (2020).","chicago":"Flynn, Sean M., Changchun Chen, Murat Artan, Stephen Barratt, Alastair Crisp, Geoffrey M. Nelson, Sew Yeu Peak-Chew, Farida Begum, Mark Skehel, and Mario de Bono. “MALT-1 Mediates IL-17 Neural Signaling to Regulate C. Elegans Behavior, Immunity and Longevity.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15872-y\">https://doi.org/10.1038/s41467-020-15872-y</a>.","ista":"Flynn SM, Chen C, Artan M, Barratt S, Crisp A, Nelson GM, Peak-Chew SY, Begum F, Skehel M, de Bono M. 2020. MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity. Nature Communications. 11, 2099.","mla":"Flynn, Sean M., et al. “MALT-1 Mediates IL-17 Neural Signaling to Regulate C. Elegans Behavior, Immunity and Longevity.” <i>Nature Communications</i>, vol. 11, 2099, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15872-y\">10.1038/s41467-020-15872-y</a>.","ieee":"S. M. Flynn <i>et al.</i>, “MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ama":"Flynn SM, Chen C, Artan M, et al. MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15872-y\">10.1038/s41467-020-15872-y</a>"},"has_accepted_license":"1","scopus_import":"1","article_number":"2099","quality_controlled":"1","type":"journal_article","isi":1,"publisher":"Springer Nature","intvolume":"        11","status":"public","date_published":"2020-04-29T00:00:00Z","publication_status":"published","abstract":[{"text":"Besides pro-inflammatory roles, the ancient cytokine interleukin-17 (IL-17) modulates neural circuit function. We investigate IL-17 signaling in neurons, and the extent it can alter organismal phenotypes. We combine immunoprecipitation and mass spectrometry to biochemically characterize endogenous signaling complexes that function downstream of IL-17 receptors in C. elegans neurons. We identify the paracaspase MALT-1 as a critical output of the pathway. MALT1 mediates signaling from many immune receptors in mammals, but was not previously implicated in IL-17 signaling or nervous system function. C. elegans MALT-1 forms a complex with homologs of Act1 and IRAK and appears to function both as a scaffold and a protease. MALT-1 is expressed broadly in the C. elegans nervous system, and neuronal IL-17–MALT-1 signaling regulates multiple phenotypes, including escape behavior, associative learning, immunity and longevity. Our data suggest MALT1 has an ancient role modulating neural circuit function downstream of IL-17 to remodel physiology and behavior.","lang":"eng"}],"ddc":["570"],"pmid":1,"doi":"10.1038/s41467-020-15872-y","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"04","language":[{"iso":"eng"}],"oa":1,"oa_version":"Published Version","date_updated":"2026-04-03T09:27:08Z","file":[{"creator":"dernst","access_level":"open_access","date_updated":"2020-07-14T12:48:03Z","content_type":"application/pdf","relation":"main_file","checksum":"dce367abf2c1a1d15f58fe6f7de82893","date_created":"2020-05-11T10:36:33Z","file_id":"7817","file_size":4609120,"file_name":"2020_NatureComm_Flynn.pdf"}],"corr_author":"1","day":"29"}]