[{"_id":"21711","scopus_import":"1","file_date_updated":"2026-05-04T10:31:35Z","acknowledgement":"The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel Nicolas, Institute for Science and Technology Austria, for their support. This research was conducted during a student residency in Vienna under the auspices of OeAD. ZI: ICM-2016-03196.","day":"01","article_number":"10","issue":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses","OA_place":"publisher","PlanS_conform":"1","date_updated":"2026-05-04T10:36:21Z","citation":{"apa":"Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., &#38; Mitulović, G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. MDPI. <a href=\"https://doi.org/10.3390/proteomes14010010\">https://doi.org/10.3390/proteomes14010010</a>","chicago":"Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković, and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>. MDPI, 2026. <a href=\"https://doi.org/10.3390/proteomes14010010\">https://doi.org/10.3390/proteomes14010010</a>.","ama":"Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. 2026;14(1). doi:<a href=\"https://doi.org/10.3390/proteomes14010010\">10.3390/proteomes14010010</a>","ista":"Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.","short":"J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović, Proteomes 14 (2026).","ieee":"J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović, “Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses,” <i>Proteomes</i>, vol. 14, no. 1. MDPI, 2026.","mla":"Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>, vol. 14, no. 1, 10, MDPI, 2026, doi:<a href=\"https://doi.org/10.3390/proteomes14010010\">10.3390/proteomes14010010</a>."},"oa":1,"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"file_name":"2026_Proteomes_Vukajlovic.pdf","date_updated":"2026-05-04T10:31:35Z","date_created":"2026-05-04T10:31:35Z","access_level":"open_access","relation":"main_file","creator":"dernst","file_size":1009723,"content_type":"application/pdf","file_id":"21790","checksum":"1e0c66bbf4b6e0be626a8639ea664b63","success":1}],"type":"journal_article","month":"03","pmid":1,"author":[{"last_name":"Miletić Vukajlović","first_name":"Jadranka","full_name":"Miletić Vukajlović, Jadranka"},{"full_name":"Ilić, Bojana","last_name":"Ilić","first_name":"Bojana"},{"full_name":"Bruszel, Bella","id":"70abbbb3-88ea-11ec-8e0a-e8c939944834","first_name":"Bella","last_name":"Bruszel"},{"full_name":"Panić-Janković, Tanja","last_name":"Panić-Janković","first_name":"Tanja"},{"full_name":"Mitulović, Goran","last_name":"Mitulović","first_name":"Goran"}],"publication_status":"published","date_created":"2026-04-12T22:01:49Z","external_id":{"pmid":["41893725"]},"department":[{"_id":"MassSpec"}],"OA_type":"gold","intvolume":"        14","publication":"Proteomes","date_published":"2026-03-01T00:00:00Z","volume":14,"language":[{"iso":"eng"}],"DOAJ_listed":"1","article_processing_charge":"Yes","publisher":"MDPI","abstract":[{"text":"Background: Low-volume trapping columns are essential for sample enrichment, desalting, and injection profile focusing on nano-LC–MS-based proteomics. They enable higher sample loading, improve chromatographic performance, and protect the analytical column by removing salts and contaminants. Recently, monolithic trap columns with micropillar architecture have emerged as alternatives to conventionally packed traps. This study compares the performance of a packed and a micropillar monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic digest of HeLa cell lysate was analyzed under identical LC–MS conditions using both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities was performed in Perseus using Welch’s t-test and clustering analysis. Results: Over 2500 proteins were identified with both setups. The packed trap column yielded more total peptides, particularly those with post-translational modifications and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column design significantly influences peptide recovery and proteome coverage.","lang":"eng"}],"has_accepted_license":"1","quality_controlled":"1","doi":"10.3390/proteomes14010010","publication_identifier":{"eissn":["2227-7382"]},"article_type":"original","ddc":["540"],"oa_version":"Published Version","year":"2026"},{"scopus_import":"1","file_date_updated":"2026-05-07T08:21:06Z","_id":"21779","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"4","article_number":"e70357","day":"01","acknowledgement":"This work was supported by the University of Vienna. The authors thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR Centre (both of the Faculty of Chemistry, University of Vienna and members of the Vienna Life Science Instruments) for assistance with data acquisition. Open Access funding provided by Universitat Wien. This work was supported by Universität Wien.","OA_place":"publisher","title":"Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin","oa":1,"citation":{"ista":"Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. 19(4), e70357.","short":"A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke, R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).","ieee":"A. Vignolle <i>et al.</i>, “Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,” <i>Microbial Biotechnology</i>, vol. 19, no. 4. Wiley, 2026.","mla":"Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>, vol. 19, no. 4, e70357, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>.","apa":"Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke, U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. Wiley. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>","chicago":"Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider, Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>.","ama":"Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. 2026;19(4). doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>"},"date_updated":"2026-05-07T08:22:41Z","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","month":"04","type":"journal_article","file":[{"date_created":"2026-05-07T08:21:06Z","date_updated":"2026-05-07T08:21:06Z","file_name":"2026_MicrobialBiotechnology_Vignolle.pdf","relation":"main_file","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":575492,"file_id":"21835","success":1,"checksum":"8c8aa660cef5394167e06f187adbabf0"}],"publication_status":"published","author":[{"last_name":"Vignolle","first_name":"Anna","full_name":"Vignolle, Anna"},{"id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","last_name":"Zehl","first_name":"Martin","orcid":"0000-0001-9685-0373","full_name":"Zehl, Martin"},{"full_name":"Garzón, Jaime Felipe Guerrero","last_name":"Garzón","first_name":"Jaime Felipe Guerrero"},{"last_name":"Schneider","first_name":"Olha","full_name":"Schneider, Olha"},{"first_name":"Johannes","last_name":"Gafriller","full_name":"Gafriller, Johannes"},{"last_name":"Grienke","first_name":"Ulrike","full_name":"Grienke, Ulrike"},{"full_name":"Kirkegaard, Rasmus H.","last_name":"Kirkegaard","first_name":"Rasmus H."},{"last_name":"Zotchev","first_name":"Sergey B.","full_name":"Zotchev, Sergey B."}],"pmid":1,"department":[{"_id":"MassSpec"}],"date_created":"2026-05-03T22:01:37Z","external_id":{"pmid":["42036976"]},"volume":19,"date_published":"2026-04-01T00:00:00Z","publication":"Microbial Biotechnology","intvolume":"        19","OA_type":"gold","article_processing_charge":"Yes","language":[{"iso":"eng"}],"DOAJ_listed":"1","abstract":[{"text":"Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action, targeting the biotin biosynthesis pathway. Despite being highly active against mycobacteria in vitro, its development as an anti-tubercular agent has been hindered due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis may yield new analogues with improved pharmacological properties. Here, we describe the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary metabolites targeting a different enzyme in the biotin biosynthesis pathway, and two genes for streptavidins, proteins that strongly bind and sequester biotin. The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous expression, which suggested that the fatty acid required for the formation of acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in the acidomycin BGC resulted in a significant decrease in its yield but did not abrogate the biosynthesis completely.","lang":"eng"}],"publisher":"Wiley","doi":"10.1111/1751-7915.70357","quality_controlled":"1","has_accepted_license":"1","publication_identifier":{"eissn":["1751-7915"]},"oa_version":"Published Version","ddc":["570"],"article_type":"original","year":"2026"},{"date_updated":"2026-06-10T07:49:04Z","PlanS_conform":"1","oa":1,"citation":{"ista":"Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17, 1793713.","short":"I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba, J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026).","ieee":"I. Tocino-Márquez <i>et al.</i>, “Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments,” <i>Frontiers in Microbiology</i>, vol. 17. Frontiers Media, 2026.","mla":"Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>, vol. 17, 1793713, Frontiers Media, 2026, doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>.","apa":"Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba, J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>","ama":"Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. 2026;17. doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>","chicago":"Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca, Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B. Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>. Frontiers Media, 2026. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>."},"OA_place":"publisher","title":"Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments","day":"20","article_number":"1793713","acknowledgement":"The computational results of this work have been achieved using the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally thank Julia Ramesmayer for assistance during DNA extraction and sample preparation for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by University of Vienna. ","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21953","file_date_updated":"2026-06-10T07:46:30Z","date_created":"2026-06-08T08:34:10Z","external_id":{"pmid":["42088272"]},"department":[{"_id":"MassSpec"}],"author":[{"first_name":"Inmaculada","last_name":"Tocino-Márquez","full_name":"Tocino-Márquez, Inmaculada"},{"full_name":"Zehl, Martin","last_name":"Zehl","orcid":"0000-0001-9685-0373","first_name":"Martin","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55"},{"first_name":"Jovana","last_name":"Batajic","full_name":"Batajic, Jovana"},{"full_name":"Séneca, Joana","first_name":"Joana","last_name":"Séneca"},{"full_name":"Pjevac, Petra","last_name":"Pjevac","first_name":"Petra"},{"full_name":"Murillo-Alba, José","last_name":"Murillo-Alba","first_name":"José"},{"full_name":"Martín, Jesús","first_name":"Jesús","last_name":"Martín"},{"full_name":"Sekurova, Olga N.","last_name":"Sekurova","first_name":"Olga N."},{"full_name":"Zotchev, Sergey B.","first_name":"Sergey B.","last_name":"Zotchev"}],"pmid":1,"publication_status":"published","type":"journal_article","month":"04","file":[{"file_size":3582644,"content_type":"application/pdf","success":1,"file_id":"21989","checksum":"31fb6b98c8a6d4007cb21808c6d2d9e3","creator":"dernst","file_name":"2026_FrontiersMicrobiology_TocinoMarquez.pdf","date_updated":"2026-06-10T07:46:30Z","date_created":"2026-06-10T07:46:30Z","access_level":"open_access","relation":"main_file"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","publisher":"Frontiers Media","abstract":[{"lang":"eng","text":"Several Streptomyces strains were isolated from freshwater sediments collected in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses revealed biosynthetic gene clusters (BGCs) that may specify production of chemically diverse secondary metabolites. Various culture conditions were employed to induce metabolite production, and subsequent LC-MS analyses facilitated the identification of the produced compounds and their correlation with the corresponding BGCs. These analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic potential, revealing a diverse range of bioactive secondary metabolites, including siderophores, antibiotics, and other compounds with potential therapeutic applications. Genomes of two Streptomyces isolates, one of them representing a potentially new species, harbored several uncharacterized BGCs that may specify biosynthesis of novel secondary metabolites. Although targeted overexpression of pathway-specific regulators from these BGCs did not yield additional metabolites, whereas knockout experiments led to metabolic changes, presumably reflecting regulatory or compensatory interactions between multiple biosynthetic pathways. Continued exploration of these strains and their BGCs may lead to the discovery of new bioactive molecules with pharmaceutical and biotechnological applications."}],"article_processing_charge":"Yes","DOAJ_listed":"1","language":[{"iso":"eng"}],"intvolume":"        17","OA_type":"gold","volume":17,"publication":"Frontiers in Microbiology","date_published":"2026-04-20T00:00:00Z","year":"2026","article_type":"original","ddc":["572"],"oa_version":"Published Version","publication_identifier":{"issn":["1664-302X"]},"has_accepted_license":"1","doi":"10.3389/fmicb.2026.1793713","quality_controlled":"1"},{"publication_status":"published","author":[{"full_name":"Kralova, Stanislava","first_name":"Stanislava","last_name":"Kralova"},{"full_name":"Spacek, Peter","last_name":"Spacek","first_name":"Peter"},{"full_name":"Gafriller, Johannes","first_name":"Johannes","last_name":"Gafriller"},{"last_name":"Bezdicek","first_name":"Matej","full_name":"Bezdicek, Matej"},{"full_name":"Medvedcova, Viktoria","first_name":"Viktoria","last_name":"Medvedcova"},{"full_name":"Séneca, Joana","first_name":"Joana","last_name":"Séneca"},{"full_name":"Osvatic, Jay","first_name":"Jay","last_name":"Osvatic"},{"last_name":"Grienke","first_name":"Ulrike","full_name":"Grienke, Ulrike"},{"last_name":"Rattei","first_name":"Thomas","full_name":"Rattei, Thomas"},{"first_name":"Olga N.","last_name":"Sekurova","full_name":"Sekurova, Olga N."},{"full_name":"Zotchev, Sergey B.","last_name":"Zotchev","first_name":"Sergey B."},{"orcid":"0000-0001-9685-0373","first_name":"Martin","last_name":"Zehl","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","full_name":"Zehl, Martin"},{"full_name":"Loy, Alexander","first_name":"Alexander","last_name":"Loy"}],"pmid":1,"department":[{"_id":"MassSpec"}],"date_created":"2026-07-08T09:19:43Z","external_id":{"biorxivid":["10.64898/2026.02.23.707395"],"pmid":["42210522"]},"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"keyword":["Actinokineospora","Antarctica","antimicrobial discovery","biosynthetic gene cluster","genome mining","microbial competition","nonribosomalpeptide synthetase","siderophores"],"file":[{"file_id":"22271","success":1,"checksum":"4f735714644f1049b22b014225843d8d","content_type":"application/pdf","file_size":2497486,"creator":"dernst","relation":"main_file","access_level":"open_access","date_created":"2026-07-13T06:57:19Z","date_updated":"2026-07-13T06:57:19Z","file_name":"2026_MicrobialBiotechnology_Kralova.pdf"}],"type":"journal_article","month":"06","corr_author":"1","supplementarymaterial":"yes","title":"Kineochelins - A new group of siderophores from an antarctic bacterium","OA_place":"publisher","oa":1,"citation":{"ama":"Kralova S, Spacek P, Gafriller J, et al. Kineochelins - A new group of siderophores from an antarctic bacterium. <i>Microbial Biotechnology</i>. 2026;19(6). doi:<a href=\"https://doi.org/10.1111/1751-7915.70386\">10.1111/1751-7915.70386</a>","chicago":"Kralova, Stanislava, Peter Spacek, Johannes Gafriller, Matej Bezdicek, Viktoria Medvedcova, Joana Séneca, Jay Osvatic, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” <i>Microbial Biotechnology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/1751-7915.70386\">https://doi.org/10.1111/1751-7915.70386</a>.","apa":"Kralova, S., Spacek, P., Gafriller, J., Bezdicek, M., Medvedcova, V., Séneca, J., … Loy, A. (2026). Kineochelins - A new group of siderophores from an antarctic bacterium. <i>Microbial Biotechnology</i>. Wiley. <a href=\"https://doi.org/10.1111/1751-7915.70386\">https://doi.org/10.1111/1751-7915.70386</a>","ieee":"S. Kralova <i>et al.</i>, “Kineochelins - A new group of siderophores from an antarctic bacterium,” <i>Microbial Biotechnology</i>, vol. 19, no. 6. Wiley, 2026.","mla":"Kralova, Stanislava, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” <i>Microbial Biotechnology</i>, vol. 19, no. 6, e70386, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/1751-7915.70386\">10.1111/1751-7915.70386</a>.","short":"S. Kralova, P. Spacek, J. Gafriller, M. Bezdicek, V. Medvedcova, J. Séneca, J. Osvatic, U. Grienke, T. Rattei, O.N. Sekurova, S.B. Zotchev, M. Zehl, A. Loy, Microbial Biotechnology 19 (2026).","ista":"Kralova S, Spacek P, Gafriller J, Bezdicek M, Medvedcova V, Séneca J, Osvatic J, Grienke U, Rattei T, Sekurova ON, Zotchev SB, Zehl M, Loy A. 2026. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 19(6), e70386."},"date_updated":"2026-07-13T06:59:08Z","scopus_import":"1","file_date_updated":"2026-07-13T06:57:19Z","_id":"22254","issue":"6","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","dataavailabilitystatement":"The genome sequence and transcriptomic data of strain Actinokineospora sp. UV203 are available on NCBI (BioProject accession number PRJNA1331526). The nearly full-length 16S rRNA gene (1395 bp) of strain Actinokineospora sp. UV203 is available on NCBI (accession number PX090945). The NMR data of kineochelin E1 and A1 are deposited in the Natural Products Magnetic Resonance Database (NP-MRD) under accession numbers NP0352113 and NP0352114, respectively.","acknowledgement":"This work was supported by the Czech Antarctic Research Programme 2025–2027 (VAN 2025) and the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). S.K. has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 101020356 (DEFCOMANT, https://doi.org/10.3030/101020356) and MASH StG/CoG (MUNI/SC/1946/2024) by Masaryk University. T.R. and A.L. were funded in part by the Austrian Science Fund FWF [grant DOI https://doi.org/10.55776/COE7]. M.B. was funded by the Ministry of Health, Czech Republic—conceptual development of research organization (FNBr, 65269705). The Life Science Compute Cluster LiSC at the University of Vienna provided the high-performance computing infrastructure for this study. We thank Julia Ramesmayer and Sara Malinowski (Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna) for assistance during high molecular weight extraction and RNA extraction. The authors thank Anna Fabisikova and Michael Klemm-Abraham from the Mass Spectrometry Centre and the team of the NMR Centre (both core facilities of the Faculty of Chemistry, University of Vienna, and members of the Vienna Life Science Instruments) for assistance with data acquisition. We are thankful to Dr. Jaime Felipe Guerrero Garzón for helpful discussions on the use of a rrn operon promoter strategy. For open access purposes, the authors have applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. Dr. Martin Kello (Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia) and Dr. Michal Goga (Department of Plant Biology, Faculty of Science and Center for Interdisciplinary Biosciences, Technology and Innovation Park, Pavol Jozef Šafárik University in Košice, Košice, Slovakia), funded by VEGA 1/0498/23, are acknowledged for their assistance with the antiproliferative assays. This work was supported by Horizon 2020 Framework Programme, 101020356; Universität Wien, MetaBac; Ministry of Education, Youth and Sports, VAN 2025; Masarykova Univerzita, MUNI/SC/1946/2024; Austrian Science Fund, 10.55776/COE7; Ministerstvo Zdravotnictví České Republiky, FNBr, 65269705; Vedecká grantová agentúra Ministerstva školstva, výskumu, vývoja a mládeže Slovenskej republiky a Slovenskej akadémie vied, VEGA 1/0498/23.","day":"01","article_number":"e70386","oa_version":"Published Version","ddc":["570"],"biorxivid":1,"article_type":"original","year":"2026","quality_controlled":"1","doi":"10.1111/1751-7915.70386","has_accepted_license":"1","publication_identifier":{"eissn":["1751-7915"]},"DOAJ_listed":"1","language":[{"iso":"eng"}],"article_processing_charge":"Yes","abstract":[{"lang":"eng","text":"The global rise of antimicrobial resistance has intensified the search for new microbial metabolites from underexplored environments and taxonomic groups. Extreme and geographically isolated habitats such as Antarctic terrestrial ecosystems represent promising reservoirs of biosynthetic diversity, particularly among rare and difficult-to-cultivate actinomycetes that may produce chemically diverse metabolites with potential biotechnological applications. Here, we report the characterization of kineochelins, a previously undescribed group of siderophores produced by the Antarctic isolate Actinokineospora sp. UV203, representing a difficult-to-cultivate actinomycete lineage. Structural elucidation revealed a set of closely related congeners with a mixed-ligand architecture consistent with metal-chelating activity. Genome mining combined with transcriptomic analysis identified a dedicated nonribosomal peptide synthetase-encoding biosynthetic gene cluster responsible for kineochelin production. Comparative genomic analyses indicated that, although kineochelin biosynthetic genes share limited similarity with known mixed-ligand siderophores, their gene content and organization differ substantially, suggesting a distinct biosynthetic lineage. Functional characterization of the culture supernatant and an enriched pre-purified kineochelin fraction demonstrated strong and selective iron chelation, with high affinity for ferric and ferrous iron. Crude culture extracts inhibited the growth of bacterial strains isolated from the same Antarctic environment, indicating that kineochelins may contribute to iron-mediated microbial competition. In addition, kineochelin-enriched pre-purified fractions showed moderate selective inhibitory activity against the opportunistic yeast pathogen Nakaseomyces glabratus and a clinical isolate of Saccharomyces cerevisiae associated with invasive infection. These findings expand the chemical and biosynthetic diversity known within the genus Actinokineospora and demonstrate that Antarctic rare actinomycetes represent valuable sources of previously unexplored natural products. The discovery of kineochelins highlights the potential of genome-guided exploration of polar microorganisms for identifying bioactive metabolites with relevance for antimicrobial discovery and biotechnology."}],"publisher":"Wiley","das_tickbox":"1","publication":"Microbial Biotechnology","date_published":"2026-06-01T00:00:00Z","volume":19,"OA_type":"gold","intvolume":"        19"},{"type":"journal_article","month":"08","page":"32199-32208","file":[{"file_name":"2025_JACS_Liu.pdf","date_updated":"2025-09-10T06:55:17Z","date_created":"2025-09-10T06:55:17Z","access_level":"open_access","relation":"main_file","creator":"dernst","file_size":9997327,"content_type":"application/pdf","success":1,"file_id":"20334","checksum":"52892fa91adadd39a1c42da9e01139a5"}],"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"date_created":"2025-09-10T05:44:03Z","external_id":{"isi":["001558320100001"]},"department":[{"_id":"MaIb"},{"_id":"MassSpec"}],"author":[{"first_name":"Yu","orcid":"0000-0001-7313-6740","last_name":"Liu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","full_name":"Liu, Yu"},{"id":"8BD9DE16-AB3C-11E9-9C8C-2A03E6697425","first_name":"Tobias","orcid":"0000-0003-1537-7436","last_name":"Kleinhanns","full_name":"Kleinhanns, Tobias"},{"id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","first_name":"Sharona","last_name":"Horta","full_name":"Horta, Sharona"},{"id":"0601cc46-c082-11ec-9b07-bb29641d1de9","first_name":"Ewelina","last_name":"Dutkiewicz","full_name":"Dutkiewicz, Ewelina"},{"last_name":"Lu","first_name":"Shaoqing","full_name":"Lu, Shaoqing"},{"last_name":"Spadaro","first_name":"Maria Chiara","full_name":"Spadaro, Maria Chiara"},{"full_name":"Genç, Aziz","last_name":"Genç","first_name":"Aziz"},{"full_name":"Chen, Lei","last_name":"Chen","first_name":"Lei"},{"first_name":"Khak Ho","last_name":"Lim","full_name":"Lim, Khak Ho"},{"full_name":"Hong, Min","first_name":"Min","last_name":"Hong"},{"full_name":"Arbiol, Jordi","first_name":"Jordi","last_name":"Arbiol"},{"orcid":"0000-0001-5013-2843","first_name":"Maria","last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87","full_name":"Ibáñez, Maria"}],"publication_status":"published","related_material":{"record":[{"status":"public","id":"22017","relation":"dissertation_contains"}]},"day":"22","acknowledgement":"M.I. acknowledges financial support from ISTA and the Werner Siemens Foundation. The Scientific Service Units (SSU) of ISTA supported this work through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF) and the Nanofabrication Facility (NNF) and the LSF Mass Spectrometry Service. The members of the Ibáñez research group are acknowledged, especially Christine Fiedler for scientific illustration and Ihor Cherniukh for valuable discussions. Y.L. acknowledges funding from the National Natural Science Foundation of China (NSFC) (Grants No. 22209034), the Innovation and Entrepreneurship Project of Overseas Returnees in Anhui Province (Grant No. 2022LCX002) and the Fundamental Research Funds for the Central Universities (JZ2024HGTB0239). K.H.L. acknowledges financial support from the National Natural Science Foundation of China (NSFC) (Grant No. 22208293). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. Authors acknowledge the Advanced Materials programme by the Spanish Government with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat de Catalunya (Project In-CAEM). The authors thank support from the project AMaDE (PID2023-149158OB-C43), funded by MCIN/AEI/10.13039/501100011033/and by “ERDF Away of making Europe”, by the “European Union”. ICN2 is supported by the Severo Ochoa program from Spanish MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat de Catalunya. ICN2 is founding member of e-DREAM. (68) M.H. acknowledges the funding from the Australian Research Council (FT230100316 and IH200100035). M.H. acknowledges the computational support from the National Computational Infrastructure (NCI) and Pawsey Supercomputing Centre, Australia.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"35","_id":"20326","file_date_updated":"2025-09-10T06:55:17Z","scopus_import":"1","date_updated":"2026-07-28T09:55:14Z","PlanS_conform":"1","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"citation":{"apa":"Liu, Y., Kleinhanns, T., Horta, S., Dutkiewicz, E., Lu, S., Spadaro, M. C., … Ibáñez, M. (2025). Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.5c11435\">https://doi.org/10.1021/jacs.5c11435</a>","ama":"Liu Y, Kleinhanns T, Horta S, et al. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. <i>Journal of the American Chemical Society</i>. 2025;147(35):32199-32208. doi:<a href=\"https://doi.org/10.1021/jacs.5c11435\">10.1021/jacs.5c11435</a>","chicago":"Liu, Yu, Tobias Kleinhanns, Sharona Horta, Ewelina Dutkiewicz, Shaoqing Lu, Maria Chiara Spadaro, Aziz Genç, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2025. <a href=\"https://doi.org/10.1021/jacs.5c11435\">https://doi.org/10.1021/jacs.5c11435</a>.","short":"Y. Liu, T. Kleinhanns, S. Horta, E. Dutkiewicz, S. Lu, M.C. Spadaro, A. Genç, L. Chen, K.H. Lim, M. Hong, J. Arbiol, M. Ibáñez, Journal of the American Chemical Society 147 (2025) 32199–32208.","ista":"Liu Y, Kleinhanns T, Horta S, Dutkiewicz E, Lu S, Spadaro MC, Genç A, Chen L, Lim KH, Hong M, Arbiol J, Ibáñez M. 2025. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical Society. 147(35), 32199–32208.","mla":"Liu, Yu, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” <i>Journal of the American Chemical Society</i>, vol. 147, no. 35, American Chemical Society, 2025, pp. 32199–208, doi:<a href=\"https://doi.org/10.1021/jacs.5c11435\">10.1021/jacs.5c11435</a>.","ieee":"Y. Liu <i>et al.</i>, “Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se,” <i>Journal of the American Chemical Society</i>, vol. 147, no. 35. American Chemical Society, pp. 32199–32208, 2025."},"oa":1,"OA_place":"publisher","title":"Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se","corr_author":"1","isi":1,"publication_identifier":{"eissn":["1520-5126"],"issn":["0002-7863"]},"has_accepted_license":"1","doi":"10.1021/jacs.5c11435","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"NanoFab"},{"_id":"MassSpec"}],"quality_controlled":"1","year":"2025","article_type":"original","oa_version":"Published Version","ddc":["540"],"intvolume":"       147","OA_type":"hybrid","volume":147,"publication":"Journal of the American Chemical Society","date_published":"2025-08-22T00:00:00Z","publisher":"American Chemical Society","abstract":[{"text":"Ag2Se is a promising n-type thermoelectric material, but its performance is limited by excessive carrier concentration, compositional inhomogeneity, and phase instability, challenges rooted in a narrow homogeneity range and uncontrolled Ag+ diffusion in the superionic phase. Here, we address these issues by exploiting liquid–solid interface reactions using CdSe complexes that remove surface excess Ag to yield stoichiometric Ag2Se and generate CdSe nanodomains that inhibit Ag+ diffusion and constrain grain growth. The resulting Ag2Se-CdSe nanocomposites exhibit a reproducible, stable figure of merit (zT) of 1.04 between 300 and 390 K. Beyond demonstrating high performance, we elucidate the interfacial chemical reactions that give rise to the observed microstructure and transport properties, providing a foundation for rationally engineering interfacial chemistry to tailor transport properties across diverse thermoelectric material systems.","lang":"eng"}],"article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}]}]