---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21711'
abstract:
- lang: eng
text: 'Background: Low-volume trapping columns are essential for sample enrichment,
desalting, and injection profile focusing on nano-LC–MS-based proteomics. They
enable higher sample loading, improve chromatographic performance, and protect
the analytical column by removing salts and contaminants. Recently, monolithic
trap columns with micropillar architecture have emerged as alternatives to conventionally
packed traps. This study compares the performance of a packed and a micropillar
monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic
digest of HeLa cell lysate was analyzed under identical LC–MS conditions using
both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on
a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database
(February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities
was performed in Perseus using Welch’s t-test and clustering analysis. Results:
Over 2500 proteins were identified with both setups. The packed trap column yielded
more total peptides, particularly those with post-translational modifications
and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate
hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping
efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column
design significantly influences peptide recovery and proteome coverage.'
acknowledgement: 'The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel
Nicolas, Institute for Science and Technology Austria, for their support. This research
was conducted during a student residency in Vienna under the auspices of OeAD. ZI:
ICM-2016-03196.'
article_number: '10'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jadranka
full_name: Miletić Vukajlović, Jadranka
last_name: Miletić Vukajlović
- first_name: Bojana
full_name: Ilić, Bojana
last_name: Ilić
- first_name: Bella
full_name: Bruszel, Bella
id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
last_name: Bruszel
- first_name: Tanja
full_name: Panić-Janković, Tanja
last_name: Panić-Janković
- first_name: Goran
full_name: Mitulović, Goran
last_name: Mitulović
citation:
ama: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison
of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar
trap columns for proteomics analyses. Proteomes. 2026;14(1). doi:10.3390/proteomes14010010
apa: Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., & Mitulović,
G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses. Proteomes. MDPI.
https://doi.org/10.3390/proteomes14010010
chicago: Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković,
and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides
on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” Proteomes.
MDPI, 2026. https://doi.org/10.3390/proteomes14010010.
ieee: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović,
“Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses,” Proteomes, vol.
14, no. 1. MDPI, 2026.
ista: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026.
Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.
mla: Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency
for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics
Analyses.” Proteomes, vol. 14, no. 1, 10, MDPI, 2026, doi:10.3390/proteomes14010010.
short: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović,
Proteomes 14 (2026).
date_created: 2026-04-12T22:01:49Z
date_published: 2026-03-01T00:00:00Z
date_updated: 2026-05-04T10:36:21Z
day: '01'
ddc:
- '540'
department:
- _id: MassSpec
doi: 10.3390/proteomes14010010
external_id:
pmid:
- '41893725'
file:
- access_level: open_access
checksum: 1e0c66bbf4b6e0be626a8639ea664b63
content_type: application/pdf
creator: dernst
date_created: 2026-05-04T10:31:35Z
date_updated: 2026-05-04T10:31:35Z
file_id: '21790'
file_name: 2026_Proteomes_Vukajlovic.pdf
file_size: 1009723
relation: main_file
success: 1
file_date_updated: 2026-05-04T10:31:35Z
has_accepted_license: '1'
intvolume: ' 14'
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proteomes
publication_identifier:
eissn:
- 2227-7382
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Comparison of the trapping efficiency for tryptic peptides on particle-packed
and micro-pillar trap columns for proteomics analyses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '21779'
abstract:
- lang: eng
text: Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action,
targeting the biotin biosynthesis pathway. Despite being highly active against
mycobacteria in vitro, its development as an anti-tubercular agent has been hindered
due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis
may yield new analogues with improved pharmacological properties. Here, we describe
the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces
bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin
BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary
metabolites targeting a different enzyme in the biotin biosynthesis pathway, and
two genes for streptavidins, proteins that strongly bind and sequester biotin.
The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous
expression, which suggested that the fatty acid required for the formation of
acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis
pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in
the acidomycin BGC resulted in a significant decrease in its yield but did not
abrogate the biosynthesis completely.
acknowledgement: This work was supported by the University of Vienna. The authors
thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR
Centre (both of the Faculty of Chemistry, University of Vienna and members of the
Vienna Life Science Instruments) for assistance with data acquisition. Open Access
funding provided by Universitat Wien. This work was supported by Universität Wien.
article_number: e70357
article_processing_charge: Yes
article_type: original
author:
- first_name: Anna
full_name: Vignolle, Anna
last_name: Vignolle
- first_name: Martin
full_name: Zehl, Martin
id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
last_name: Zehl
orcid: 0000-0001-9685-0373
- first_name: Jaime Felipe Guerrero
full_name: Garzón, Jaime Felipe Guerrero
last_name: Garzón
- first_name: Olha
full_name: Schneider, Olha
last_name: Schneider
- first_name: Johannes
full_name: Gafriller, Johannes
last_name: Gafriller
- first_name: Ulrike
full_name: Grienke, Ulrike
last_name: Grienke
- first_name: Rasmus H.
full_name: Kirkegaard, Rasmus H.
last_name: Kirkegaard
- first_name: Sergey B.
full_name: Zotchev, Sergey B.
last_name: Zotchev
citation:
ama: Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation
of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic
acidomycin. Microbial Biotechnology. 2026;19(4). doi:10.1111/1751-7915.70357
apa: Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke,
U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster
governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial
Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70357
chicago: Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider,
Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev.
“Identification and Characterisation of the Gene Cluster Governing Biosynthesis
of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial Biotechnology.
Wiley, 2026. https://doi.org/10.1111/1751-7915.70357.
ieee: A. Vignolle et al., “Identification and characterisation of the gene
cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,”
Microbial Biotechnology, vol. 19, no. 4. Wiley, 2026.
ista: Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard
RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster
governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial
Biotechnology. 19(4), e70357.
mla: Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster
Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial
Biotechnology, vol. 19, no. 4, e70357, Wiley, 2026, doi:10.1111/1751-7915.70357.
short: A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke,
R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).
date_created: 2026-05-03T22:01:37Z
date_published: 2026-04-01T00:00:00Z
date_updated: 2026-05-07T08:22:41Z
day: '01'
ddc:
- '570'
department:
- _id: MassSpec
doi: 10.1111/1751-7915.70357
external_id:
pmid:
- '42036976'
file:
- access_level: open_access
checksum: 8c8aa660cef5394167e06f187adbabf0
content_type: application/pdf
creator: dernst
date_created: 2026-05-07T08:21:06Z
date_updated: 2026-05-07T08:21:06Z
file_id: '21835'
file_name: 2026_MicrobialBiotechnology_Vignolle.pdf
file_size: 575492
relation: main_file
success: 1
file_date_updated: 2026-05-07T08:21:06Z
has_accepted_license: '1'
intvolume: ' 19'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Microbial Biotechnology
publication_identifier:
eissn:
- 1751-7915
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification and characterisation of the gene cluster governing biosynthesis
of the anti-mycobacterial antibiotic acidomycin
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21953'
abstract:
- lang: eng
text: Several Streptomyces strains were isolated from freshwater sediments collected
in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses
revealed biosynthetic gene clusters (BGCs) that may specify production of chemically
diverse secondary metabolites. Various culture conditions were employed to induce
metabolite production, and subsequent LC-MS analyses facilitated the identification
of the produced compounds and their correlation with the corresponding BGCs. These
analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic
potential, revealing a diverse range of bioactive secondary metabolites, including
siderophores, antibiotics, and other compounds with potential therapeutic applications.
Genomes of two Streptomyces isolates, one of them representing a potentially new
species, harbored several uncharacterized BGCs that may specify biosynthesis of
novel secondary metabolites. Although targeted overexpression of pathway-specific
regulators from these BGCs did not yield additional metabolites, whereas knockout
experiments led to metabolic changes, presumably reflecting regulatory or compensatory
interactions between multiple biosynthetic pathways. Continued exploration of
these strains and their BGCs may lead to the discovery of new bioactive molecules
with pharmaceutical and biotechnological applications.
acknowledgement: "The computational results of this work have been achieved using
the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally
thank Julia Ramesmayer for assistance during DNA extraction and sample preparation
for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty
of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s)
declared that financial support was received for this work and/or its publication.
This work was supported by the University of Vienna via the Research Platform Secondary
Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by
University of Vienna. "
article_number: '1793713'
article_processing_charge: Yes
article_type: original
author:
- first_name: Inmaculada
full_name: Tocino-Márquez, Inmaculada
last_name: Tocino-Márquez
- first_name: Martin
full_name: Zehl, Martin
id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
last_name: Zehl
orcid: 0000-0001-9685-0373
- first_name: Jovana
full_name: Batajic, Jovana
last_name: Batajic
- first_name: Joana
full_name: Séneca, Joana
last_name: Séneca
- first_name: Petra
full_name: Pjevac, Petra
last_name: Pjevac
- first_name: José
full_name: Murillo-Alba, José
last_name: Murillo-Alba
- first_name: Jesús
full_name: Martín, Jesús
last_name: Martín
- first_name: Olga N.
full_name: Sekurova, Olga N.
last_name: Sekurova
- first_name: Sergey B.
full_name: Zotchev, Sergey B.
last_name: Zotchev
citation:
ama: Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary
metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology.
2026;17. doi:10.3389/fmicb.2026.1793713
apa: Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba,
J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of
Streptomyces spp. from freshwater sediments. Frontiers in Microbiology.
Frontiers Media. https://doi.org/10.3389/fmicb.2026.1793713
chicago: Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca,
Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B.
Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp.
from Freshwater Sediments.” Frontiers in Microbiology. Frontiers Media,
2026. https://doi.org/10.3389/fmicb.2026.1793713.
ieee: I. Tocino-Márquez et al., “Unveiling the genomes and secondary metabolomes
of Streptomyces spp. from freshwater sediments,” Frontiers in Microbiology,
vol. 17. Frontiers Media, 2026.
ista: Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín
J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes
of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17,
1793713.
mla: Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes
of Streptomyces Spp. from Freshwater Sediments.” Frontiers in Microbiology,
vol. 17, 1793713, Frontiers Media, 2026, doi:10.3389/fmicb.2026.1793713.
short: I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba,
J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026).
date_created: 2026-06-08T08:34:10Z
date_published: 2026-04-20T00:00:00Z
date_updated: 2026-06-10T07:49:04Z
day: '20'
ddc:
- '572'
department:
- _id: MassSpec
doi: 10.3389/fmicb.2026.1793713
external_id:
pmid:
- '42088272'
file:
- access_level: open_access
checksum: 31fb6b98c8a6d4007cb21808c6d2d9e3
content_type: application/pdf
creator: dernst
date_created: 2026-06-10T07:46:30Z
date_updated: 2026-06-10T07:46:30Z
file_id: '21989'
file_name: 2026_FrontiersMicrobiology_TocinoMarquez.pdf
file_size: 3582644
relation: main_file
success: 1
file_date_updated: 2026-06-10T07:46:30Z
has_accepted_license: '1'
intvolume: ' 17'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Microbiology
publication_identifier:
issn:
- 1664-302X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
status: public
title: Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater
sediments
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '22254'
abstract:
- lang: eng
text: The global rise of antimicrobial resistance has intensified the search for
new microbial metabolites from underexplored environments and taxonomic groups.
Extreme and geographically isolated habitats such as Antarctic terrestrial ecosystems
represent promising reservoirs of biosynthetic diversity, particularly among rare
and difficult-to-cultivate actinomycetes that may produce chemically diverse metabolites
with potential biotechnological applications. Here, we report the characterization
of kineochelins, a previously undescribed group of siderophores produced by the
Antarctic isolate Actinokineospora sp. UV203, representing a difficult-to-cultivate
actinomycete lineage. Structural elucidation revealed a set of closely related
congeners with a mixed-ligand architecture consistent with metal-chelating activity.
Genome mining combined with transcriptomic analysis identified a dedicated nonribosomal
peptide synthetase-encoding biosynthetic gene cluster responsible for kineochelin
production. Comparative genomic analyses indicated that, although kineochelin
biosynthetic genes share limited similarity with known mixed-ligand siderophores,
their gene content and organization differ substantially, suggesting a distinct
biosynthetic lineage. Functional characterization of the culture supernatant and
an enriched pre-purified kineochelin fraction demonstrated strong and selective
iron chelation, with high affinity for ferric and ferrous iron. Crude culture
extracts inhibited the growth of bacterial strains isolated from the same Antarctic
environment, indicating that kineochelins may contribute to iron-mediated microbial
competition. In addition, kineochelin-enriched pre-purified fractions showed moderate
selective inhibitory activity against the opportunistic yeast pathogen Nakaseomyces
glabratus and a clinical isolate of Saccharomyces cerevisiae associated with invasive
infection. These findings expand the chemical and biosynthetic diversity known
within the genus Actinokineospora and demonstrate that Antarctic rare actinomycetes
represent valuable sources of previously unexplored natural products. The discovery
of kineochelins highlights the potential of genome-guided exploration of polar
microorganisms for identifying bioactive metabolites with relevance for antimicrobial
discovery and biotechnology.
acknowledgement: This work was supported by the Czech Antarctic Research Programme
2025–2027 (VAN 2025) and the University of Vienna via the Research Platform Secondary
Metabolomes of Bacterial Communities (MetaBac). S.K. has received funding from the
European Union's Horizon 2020 research and innovation programme under the Marie
Skłodowska-Curie grant agreement No. 101020356 (DEFCOMANT, https://doi.org/10.3030/101020356)
and MASH StG/CoG (MUNI/SC/1946/2024) by Masaryk University. T.R. and A.L. were funded
in part by the Austrian Science Fund FWF [grant DOI https://doi.org/10.55776/COE7].
M.B. was funded by the Ministry of Health, Czech Republic—conceptual development
of research organization (FNBr, 65269705). The Life Science Compute Cluster LiSC
at the University of Vienna provided the high-performance computing infrastructure
for this study. We thank Julia Ramesmayer and Sara Malinowski (Joint Microbiome
Facility of the Medical University of Vienna and the University of Vienna) for assistance
during high molecular weight extraction and RNA extraction. The authors thank Anna
Fabisikova and Michael Klemm-Abraham from the Mass Spectrometry Centre and the team
of the NMR Centre (both core facilities of the Faculty of Chemistry, University
of Vienna, and members of the Vienna Life Science Instruments) for assistance with
data acquisition. We are thankful to Dr. Jaime Felipe Guerrero Garzón for helpful
discussions on the use of a rrn operon promoter strategy. For open access purposes,
the authors have applied for a CC BY public copyright licence to any author-accepted
manuscript version arising from this submission. Dr. Martin Kello (Department of
Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia)
and Dr. Michal Goga (Department of Plant Biology, Faculty of Science and Center
for Interdisciplinary Biosciences, Technology and Innovation Park, Pavol Jozef Šafárik
University in Košice, Košice, Slovakia), funded by VEGA 1/0498/23, are acknowledged
for their assistance with the antiproliferative assays. This work was supported
by Horizon 2020 Framework Programme, 101020356; Universität Wien, MetaBac; Ministry
of Education, Youth and Sports, VAN 2025; Masarykova Univerzita, MUNI/SC/1946/2024;
Austrian Science Fund, 10.55776/COE7; Ministerstvo Zdravotnictví České Republiky,
FNBr, 65269705; Vedecká grantová agentúra Ministerstva školstva, výskumu, vývoja
a mládeže Slovenskej republiky a Slovenskej akadémie vied, VEGA 1/0498/23.
article_number: e70386
article_processing_charge: Yes
article_type: original
author:
- first_name: Stanislava
full_name: Kralova, Stanislava
last_name: Kralova
- first_name: Peter
full_name: Spacek, Peter
last_name: Spacek
- first_name: Johannes
full_name: Gafriller, Johannes
last_name: Gafriller
- first_name: Matej
full_name: Bezdicek, Matej
last_name: Bezdicek
- first_name: Viktoria
full_name: Medvedcova, Viktoria
last_name: Medvedcova
- first_name: Joana
full_name: Séneca, Joana
last_name: Séneca
- first_name: Jay
full_name: Osvatic, Jay
last_name: Osvatic
- first_name: Ulrike
full_name: Grienke, Ulrike
last_name: Grienke
- first_name: Thomas
full_name: Rattei, Thomas
last_name: Rattei
- first_name: Olga N.
full_name: Sekurova, Olga N.
last_name: Sekurova
- first_name: Sergey B.
full_name: Zotchev, Sergey B.
last_name: Zotchev
- first_name: Martin
full_name: Zehl, Martin
id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
last_name: Zehl
orcid: 0000-0001-9685-0373
- first_name: Alexander
full_name: Loy, Alexander
last_name: Loy
biorxivid: 1
citation:
ama: Kralova S, Spacek P, Gafriller J, et al. Kineochelins - A new group of siderophores
from an antarctic bacterium. Microbial Biotechnology. 2026;19(6). doi:10.1111/1751-7915.70386
apa: Kralova, S., Spacek, P., Gafriller, J., Bezdicek, M., Medvedcova, V., Séneca,
J., … Loy, A. (2026). Kineochelins - A new group of siderophores from an antarctic
bacterium. Microbial Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70386
chicago: Kralova, Stanislava, Peter Spacek, Johannes Gafriller, Matej Bezdicek,
Viktoria Medvedcova, Joana Séneca, Jay Osvatic, et al. “Kineochelins - A New Group
of Siderophores from an Antarctic Bacterium.” Microbial Biotechnology.
Wiley, 2026. https://doi.org/10.1111/1751-7915.70386.
ieee: S. Kralova et al., “Kineochelins - A new group of siderophores from
an antarctic bacterium,” Microbial Biotechnology, vol. 19, no. 6. Wiley,
2026.
ista: Kralova S, Spacek P, Gafriller J, Bezdicek M, Medvedcova V, Séneca J, Osvatic
J, Grienke U, Rattei T, Sekurova ON, Zotchev SB, Zehl M, Loy A. 2026. Kineochelins
- A new group of siderophores from an antarctic bacterium. Microbial Biotechnology.
19(6), e70386.
mla: Kralova, Stanislava, et al. “Kineochelins - A New Group of Siderophores from
an Antarctic Bacterium.” Microbial Biotechnology, vol. 19, no. 6, e70386,
Wiley, 2026, doi:10.1111/1751-7915.70386.
short: S. Kralova, P. Spacek, J. Gafriller, M. Bezdicek, V. Medvedcova, J. Séneca,
J. Osvatic, U. Grienke, T. Rattei, O.N. Sekurova, S.B. Zotchev, M. Zehl, A. Loy,
Microbial Biotechnology 19 (2026).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: The genome sequence and transcriptomic data of strain Actinokineospora
sp. UV203 are available on NCBI (BioProject accession number PRJNA1331526). The
nearly full-length 16S rRNA gene (1395 bp) of strain Actinokineospora sp. UV203
is available on NCBI (accession number PX090945). The NMR data of kineochelin E1
and A1 are deposited in the Natural Products Magnetic Resonance Database (NP-MRD)
under accession numbers NP0352113 and NP0352114, respectively.
date_created: 2026-07-08T09:19:43Z
date_published: 2026-06-01T00:00:00Z
date_updated: 2026-07-13T06:59:08Z
day: '01'
ddc:
- '570'
department:
- _id: MassSpec
doi: 10.1111/1751-7915.70386
external_id:
biorxivid:
- 10.64898/2026.02.23.707395
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- '42210522'
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intvolume: ' 19'
issue: '6'
keyword:
- Actinokineospora
- Antarctica
- antimicrobial discovery
- biosynthetic gene cluster
- genome mining
- microbial competition
- nonribosomalpeptide synthetase
- siderophores
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Microbial Biotechnology
publication_identifier:
eissn:
- 1751-7915
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Kineochelins - A new group of siderophores from an antarctic bacterium
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
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year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
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_id: '20326'
abstract:
- lang: eng
text: Ag2Se is a promising n-type thermoelectric material, but its performance is
limited by excessive carrier concentration, compositional inhomogeneity, and phase
instability, challenges rooted in a narrow homogeneity range and uncontrolled
Ag+ diffusion in the superionic phase. Here, we address these issues by exploiting
liquid–solid interface reactions using CdSe complexes that remove surface excess
Ag to yield stoichiometric Ag2Se and generate CdSe nanodomains that inhibit Ag+
diffusion and constrain grain growth. The resulting Ag2Se-CdSe nanocomposites
exhibit a reproducible, stable figure of merit (zT) of 1.04 between 300 and 390
K. Beyond demonstrating high performance, we elucidate the interfacial chemical
reactions that give rise to the observed microstructure and transport properties,
providing a foundation for rationally engineering interfacial chemistry to tailor
transport properties across diverse thermoelectric material systems.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: NanoFab
- _id: MassSpec
acknowledgement: 'M.I. acknowledges financial support from ISTA and the Werner Siemens
Foundation. The Scientific Service Units (SSU) of ISTA supported this work through
resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility
(LSF) and the Nanofabrication Facility (NNF) and the LSF Mass Spectrometry Service.
The members of the Ibáñez research group are acknowledged, especially Christine
Fiedler for scientific illustration and Ihor Cherniukh for valuable discussions.
Y.L. acknowledges funding from the National Natural Science Foundation of China
(NSFC) (Grants No. 22209034), the Innovation and Entrepreneurship Project of Overseas
Returnees in Anhui Province (Grant No. 2022LCX002) and the Fundamental Research
Funds for the Central Universities (JZ2024HGTB0239). K.H.L. acknowledges financial
support from the National Natural Science Foundation of China (NSFC) (Grant No.
22208293). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457.
Authors acknowledge the Advanced Materials programme by the Spanish Government with
funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat de
Catalunya (Project In-CAEM). The authors thank support from the project AMaDE (PID2023-149158OB-C43),
funded by MCIN/AEI/10.13039/501100011033/and by “ERDF Away of making Europe”, by
the “European Union”. ICN2 is supported by the Severo Ochoa program from Spanish
MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat
de Catalunya. ICN2 is founding member of e-DREAM. (68) M.H. acknowledges the funding
from the Australian Research Council (FT230100316 and IH200100035). M.H. acknowledges
the computational support from the National Computational Infrastructure (NCI) and
Pawsey Supercomputing Centre, Australia.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yu
full_name: Liu, Yu
id: 2A70014E-F248-11E8-B48F-1D18A9856A87
last_name: Liu
orcid: 0000-0001-7313-6740
- first_name: Tobias
full_name: Kleinhanns, Tobias
id: 8BD9DE16-AB3C-11E9-9C8C-2A03E6697425
last_name: Kleinhanns
orcid: 0000-0003-1537-7436
- first_name: Sharona
full_name: Horta, Sharona
id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc
last_name: Horta
- first_name: Ewelina
full_name: Dutkiewicz, Ewelina
id: 0601cc46-c082-11ec-9b07-bb29641d1de9
last_name: Dutkiewicz
- first_name: Shaoqing
full_name: Lu, Shaoqing
last_name: Lu
- first_name: Maria Chiara
full_name: Spadaro, Maria Chiara
last_name: Spadaro
- first_name: Aziz
full_name: Genç, Aziz
last_name: Genç
- first_name: Lei
full_name: Chen, Lei
last_name: Chen
- first_name: Khak Ho
full_name: Lim, Khak Ho
last_name: Lim
- first_name: Min
full_name: Hong, Min
last_name: Hong
- first_name: Jordi
full_name: Arbiol, Jordi
last_name: Arbiol
- first_name: Maria
full_name: Ibáñez, Maria
id: 43C61214-F248-11E8-B48F-1D18A9856A87
last_name: Ibáñez
orcid: 0000-0001-5013-2843
citation:
ama: Liu Y, Kleinhanns T, Horta S, et al. Liquid-solid interface reactions drive
enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical
Society. 2025;147(35):32199-32208. doi:10.1021/jacs.5c11435
apa: Liu, Y., Kleinhanns, T., Horta, S., Dutkiewicz, E., Lu, S., Spadaro, M. C.,
… Ibáñez, M. (2025). Liquid-solid interface reactions drive enhanced thermoelectric
performance in Ag2Se. Journal of the American Chemical Society. American
Chemical Society. https://doi.org/10.1021/jacs.5c11435
chicago: Liu, Yu, Tobias Kleinhanns, Sharona Horta, Ewelina Dutkiewicz, Shaoqing
Lu, Maria Chiara Spadaro, Aziz Genç, et al. “Liquid-Solid Interface Reactions
Drive Enhanced Thermoelectric Performance in Ag2Se.” Journal of the American
Chemical Society. American Chemical Society, 2025. https://doi.org/10.1021/jacs.5c11435.
ieee: Y. Liu et al., “Liquid-solid interface reactions drive enhanced thermoelectric
performance in Ag2Se,” Journal of the American Chemical Society, vol. 147,
no. 35. American Chemical Society, pp. 32199–32208, 2025.
ista: Liu Y, Kleinhanns T, Horta S, Dutkiewicz E, Lu S, Spadaro MC, Genç A, Chen
L, Lim KH, Hong M, Arbiol J, Ibáñez M. 2025. Liquid-solid interface reactions
drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical
Society. 147(35), 32199–32208.
mla: Liu, Yu, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric
Performance in Ag2Se.” Journal of the American Chemical Society, vol. 147,
no. 35, American Chemical Society, 2025, pp. 32199–208, doi:10.1021/jacs.5c11435.
short: Y. Liu, T. Kleinhanns, S. Horta, E. Dutkiewicz, S. Lu, M.C. Spadaro, A. Genç,
L. Chen, K.H. Lim, M. Hong, J. Arbiol, M. Ibáñez, Journal of the American Chemical
Society 147 (2025) 32199–32208.
corr_author: '1'
date_created: 2025-09-10T05:44:03Z
date_published: 2025-08-22T00:00:00Z
date_updated: 2026-07-28T09:55:14Z
day: '22'
ddc:
- '540'
department:
- _id: MaIb
- _id: MassSpec
doi: 10.1021/jacs.5c11435
external_id:
isi:
- '001558320100001'
file:
- access_level: open_access
checksum: 52892fa91adadd39a1c42da9e01139a5
content_type: application/pdf
creator: dernst
date_created: 2025-09-10T06:55:17Z
date_updated: 2025-09-10T06:55:17Z
file_id: '20334'
file_name: 2025_JACS_Liu.pdf
file_size: 9997327
relation: main_file
success: 1
file_date_updated: 2025-09-10T06:55:17Z
has_accepted_license: '1'
intvolume: ' 147'
isi: 1
issue: '35'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 32199-32208
project:
- _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A
name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of
Semiconductors for Waste Heat Recovery'
publication: Journal of the American Chemical Society
publication_identifier:
eissn:
- 1520-5126
issn:
- 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
related_material:
record:
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Liquid-solid interface reactions drive enhanced thermoelectric performance
in Ag2Se
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 147
year: '2025'
...