[{"quality_controlled":"1","project":[{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"}],"type":"journal_article","pmid":1,"citation":{"mla":"Liu, Jiayi, et al. “Modelling Chemotaxis of Branched Cells in Complex Environments Provides Insights into Immune Cell Navigation.” <i>PLOS Computational Biology</i>, vol. 22, no. 2, e1013934, Public Library of Science, 2026, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">10.1371/journal.pcbi.1013934</a>.","ista":"Liu J, Ron JE, Rinaldi G, Williantarra I, Georgantzoglou A, de Vries I, Sixt MK, Sarris M, Gov NS. 2026. Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. PLOS Computational Biology. 22(2), e1013934.","ama":"Liu J, Ron JE, Rinaldi G, et al. Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. <i>PLOS Computational Biology</i>. 2026;22(2). doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">10.1371/journal.pcbi.1013934</a>","ieee":"J. Liu <i>et al.</i>, “Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation,” <i>PLOS Computational Biology</i>, vol. 22, no. 2. Public Library of Science, 2026.","apa":"Liu, J., Ron, J. E., Rinaldi, G., Williantarra, I., Georgantzoglou, A., de Vries, I., … Gov, N. S. (2026). Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. <i>PLOS Computational Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">https://doi.org/10.1371/journal.pcbi.1013934</a>","chicago":"Liu, Jiayi, Jonathan E. Ron, Giulia Rinaldi, Ivanna Williantarra, Antonios Georgantzoglou, Ingrid de Vries, Michael K Sixt, Milka Sarris, and Nir S. Gov. “Modelling Chemotaxis of Branched Cells in Complex Environments Provides Insights into Immune Cell Navigation.” <i>PLOS Computational Biology</i>. Public Library of Science, 2026. <a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">https://doi.org/10.1371/journal.pcbi.1013934</a>.","short":"J. Liu, J.E. Ron, G. Rinaldi, I. Williantarra, A. Georgantzoglou, I. de Vries, M.K. Sixt, M. Sarris, N.S. Gov, PLOS Computational Biology 22 (2026)."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"OA_type":"gold","article_type":"original","article_number":"e1013934","issue":"2","has_accepted_license":"1","publication_identifier":{"eissn":["1553-7358"]},"PlanS_conform":"1","acknowledgement":"N.S.G. is the incumbent of the Lee and William Abramowitz Professorial Chair of Biophysics (Weizmann Institute), and acknowledges support from the Royal Society Wolfson Visiting Fellowship, and Human Frontier Science Program grant RGP0032/2022. Work by M.S., I.W., G.R. and A.G. was supported by the Leverhulme Trust (grant RPG-2021-226) and the European Research Council (ERC) under the Horizon 2020 program and UKRI, Grant agreement No.\r\nEP/Y02799X/1. M.S. and I.d.V acknowledge support by the European Research Council (grant ERC-SyG 101071793 to M.S). The funders had no role in study design, data collection and\r\nanalysis, decision to publish, or preparation of the manuscript.","status":"public","license":"https://creativecommons.org/licenses/by/4.0/","file":[{"success":1,"creator":"dernst","file_name":"2026_PloSCompBio_.pdf","checksum":"564041089e7334804ad3cade973f80b4","content_type":"application/pdf","relation":"main_file","file_id":"21388","date_updated":"2026-03-02T14:11:14Z","access_level":"open_access","date_created":"2026-03-02T14:11:14Z","file_size":20688452}],"file_date_updated":"2026-03-02T14:11:14Z","volume":22,"publisher":"Public Library of Science","author":[{"first_name":"Jiayi","last_name":"Liu","full_name":"Liu, Jiayi"},{"first_name":"Jonathan E.","full_name":"Ron, Jonathan E.","last_name":"Ron"},{"first_name":"Giulia","last_name":"Rinaldi","full_name":"Rinaldi, Giulia"},{"first_name":"Ivanna","last_name":"Williantarra","full_name":"Williantarra, Ivanna"},{"last_name":"Georgantzoglou","full_name":"Georgantzoglou, Antonios","first_name":"Antonios"},{"full_name":"de Vries, Ingrid","last_name":"de Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"last_name":"Sarris","full_name":"Sarris, Milka","first_name":"Milka"},{"first_name":"Nir S.","full_name":"Gov, Nir S.","last_name":"Gov"}],"_id":"21384","article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"date_published":"2026-02-03T00:00:00Z","year":"2026","day":"03","oa":1,"intvolume":"        22","month":"02","ddc":["570"],"date_created":"2026-03-02T10:08:38Z","publication":"PLOS Computational Biology","OA_place":"publisher","doi":"10.1371/journal.pcbi.1013934","abstract":[{"lang":"eng","text":"Cell migration in vivo is often guided by chemical signaling, i.e., chemotaxis. For immune cells performing chemotaxis in the organism, this process is influenced by the complex geometry of the tissue environment. In this study, we use a theoretical model of branched cell migration on a network to explore the cellular response to chemical gradients. The model predicts the response of a branched cell to a chemical gradient: how the cell reorients its internal polarity and how it navigates through a complex environment up a chemical gradient. We then compare the model’s predictions with experimental observations of neutrophils migrating to the site of a laser-inflicted wound in a zebrafish larva fin, and neutrophils migrating in vitro inside a regular lattice of pillars. We find that the model captures the details of the subcellular response to the chemokine gradient, as well as qualitative characteristics of the large-scale migration, suggesting that the neutrophils behave as fast cells, which explains the functionality of these immune cells."}],"title":"Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation","DOAJ_listed":"1","external_id":{"pmid":["41632822"]},"scopus_import":"1","publication_status":"published","date_updated":"2026-03-02T14:12:22Z","language":[{"iso":"eng"}],"oa_version":"Published Version"},{"date_created":"2026-04-12T22:01:48Z","publication":"Science Advances","month":"03","ddc":["570"],"intvolume":"        12","oa":1,"language":[{"iso":"eng"}],"oa_version":"Published Version","publication_status":"published","date_updated":"2026-05-04T09:18:06Z","scopus_import":"1","DOAJ_listed":"1","abstract":[{"text":"Structural and functional differences between brain hemispheres are a common feature of animal nervous systems with reduced bilateral asymmetry often linked to impaired cognitive performance. How neuronal left-right asymmetry is initiated and integrated into a bilaterally symmetrical ground pattern is poorly understood. Here, we show that the directional asymmetry of a Drosophila central brain circuit originates from axonal interactions of two types of bilateral pioneer neurons. Subsequent recruitment of neighboring neurons into the asymmetric neuropil primordium results in hemisphere-specific microcircuits. Circuit lateralization requires dynamic expression of the cell adhesion molecule Fasciclin 2 to maintain structural plasticity in axonal remodeling. Reduced circuit asymmetry following cell type–specific Fasciclin 2 manipulation affects adult brain function. These results reveal an unexpected degree of developmental plasticity of late-born Drosophila neurons in the formation of a circuit node via the lateralized recruitment of symmetric circuit components.","lang":"eng"}],"title":"Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity","OA_place":"publisher","doi":"10.1126/sciadv.aea6020","has_accepted_license":"1","publication_identifier":{"eissn":["2375-2548"]},"article_number":"eaea6020","issue":"13","OA_type":"gold","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","citation":{"ama":"Markovitsch JW, Mitić D, Del Pilar Jiménez García A, et al. Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. <i>Science Advances</i>. 2026;12(13). doi:<a href=\"https://doi.org/10.1126/sciadv.aea6020\">10.1126/sciadv.aea6020</a>","ista":"Markovitsch JW, Mitić D, Del Pilar Jiménez García A, Zane A, Kainz S, Kaur R, Hummel T. 2026. Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. Science Advances. 12(13), eaea6020.","chicago":"Markovitsch, Johann W., Daniel Mitić, Alisa Del Pilar Jiménez García, Alsberga Zane, Sarah Kainz, Rashmit Kaur, and Thomas Hummel. “Sequential Formation of Drosophila Circuit Asymmetry via Prolonged Structural Plasticity.” <i>Science Advances</i>. American Association for the Advancement of Science, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea6020\">https://doi.org/10.1126/sciadv.aea6020</a>.","short":"J.W. Markovitsch, D. Mitić, A. Del Pilar Jiménez García, A. Zane, S. Kainz, R. Kaur, T. Hummel, Science Advances 12 (2026).","ieee":"J. W. Markovitsch <i>et al.</i>, “Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity,” <i>Science Advances</i>, vol. 12, no. 13. American Association for the Advancement of Science, 2026.","apa":"Markovitsch, J. W., Mitić, D., Del Pilar Jiménez García, A., Zane, A., Kainz, S., Kaur, R., &#38; Hummel, T. (2026). Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aea6020\">https://doi.org/10.1126/sciadv.aea6020</a>","mla":"Markovitsch, Johann W., et al. “Sequential Formation of Drosophila Circuit Asymmetry via Prolonged Structural Plasticity.” <i>Science Advances</i>, vol. 12, no. 13, eaea6020, American Association for the Advancement of Science, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea6020\">10.1126/sciadv.aea6020</a>."},"quality_controlled":"1","type":"journal_article","date_published":"2026-03-27T00:00:00Z","year":"2026","day":"27","department":[{"_id":"MiSi"},{"_id":"GradSch"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21707","article_processing_charge":"Yes","author":[{"last_name":"Markovitsch","full_name":"Markovitsch, Johann W.","first_name":"Johann W."},{"last_name":"Mitić","full_name":"Mitić, Daniel","first_name":"Daniel"},{"last_name":"Del Pilar Jiménez García","full_name":"Del Pilar Jiménez García, Alisa","first_name":"Alisa"},{"full_name":"Zane, Alsberga","last_name":"Zane","id":"60f7509a-f652-11ea-9d86-b963d6490d7c","first_name":"Alsberga","orcid":"0009-0003-0415-7603"},{"first_name":"Sarah","last_name":"Kainz","full_name":"Kainz, Sarah"},{"first_name":"Rashmit","last_name":"Kaur","full_name":"Kaur, Rashmit"},{"last_name":"Hummel","full_name":"Hummel, Thomas","first_name":"Thomas"}],"publisher":"American Association for the Advancement of Science","volume":12,"file_date_updated":"2026-05-04T09:16:36Z","status":"public","file":[{"checksum":"3eed470fe73e53d2a8d55d6fba6934e3","file_name":"2026_ScienceAdv_Markovitsch.pdf","content_type":"application/pdf","creator":"dernst","success":1,"file_id":"21786","relation":"main_file","access_level":"open_access","date_updated":"2026-05-04T09:16:36Z","file_size":11101140,"date_created":"2026-05-04T09:16:36Z"}],"acknowledgement":"We thank I. Salecker (Flybow), B. Altenhein (Fas2-Gal4Mz507), A. Nose (UAS-intra- and extra-Fas2::YFP), and C. S. Goodman (UAS-Fas2PEST+/−), as well as the Bloomington Stock Center for providing materials and fly stocks. We thank S. Waddell and the lab, especially B. Senapati, for providing the opportunity to conduct memory experiments at the CNCB, University of Oxford, and for supervision and discussions during this period. We also thank W. Kallina, S. Ilgerl, D. Bartel, A. Grimm, and A. Litin for technical support and the Hummel Lab for stimulating discussions and critical comments on the manuscript. We acknowledge the early exploratory work of A. Mattia, S. Trkulja, C. Schönherr, S. Bogner, B. Simpson, L. Tomasek, H. Roth, H. Vokač, R. Gredler, F. Kapelari, T. Kolarova, C. Ignitsch, Á. Bautista-Soldevila, and M. Kassem.\r\nThis research was funded by the University of Vienna, the Vienna Doctoral School Cognition, Behaviour and Neuroscience (uni:docs fellowship) (to J.W.M.) and by the Austrian Science Fund (FWF) (Cluster of Excellence Neuronal Circuits in Health and Disease, grant DOI 10.55776/COE16; https://www.fwf.ac.at/en/research-radar/10.55776/COE16) (to T.H.). For open access purposes, the author has applied a CC BY public copyright license to any author-accepted manuscript version arising from this submission."},{"OA_type":"hybrid","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","citation":{"chicago":"Gawish, Riem, Rajagopal Varada, Florian Deckert, Anastasiya Hladik, Linda Steinbichl, Laura Cimatti, Katarina Milanovic, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>. Rockefeller University Press, 2025. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>.","short":"R. Gawish, R. Varada, F. Deckert, A. Hladik, L. Steinbichl, L. Cimatti, K. Milanovic, M. Jain, N. Torgasheva, A. Tanzer, K. De Paepe, T. Van De Wiele, B. Hausmann, M. Lang, M. Pechhacker, N. Ibrahim, I. de Vries, C. Brostjan, M.K. Sixt, C. Gasche, L. Boon, D. Berry, M.F. Jantsch, F.C. Pereira, C. Vesely, Journal of Experimental Medicine 222 (2025).","ieee":"R. Gawish <i>et al.</i>, “Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis,” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9. Rockefeller University Press, 2025.","apa":"Gawish, R., Varada, R., Deckert, F., Hladik, A., Steinbichl, L., Cimatti, L., … Vesely, C. (2025). Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>","ama":"Gawish R, Varada R, Deckert F, et al. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. 2025;222(9). doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>","ista":"Gawish R, Varada R, Deckert F, Hladik A, Steinbichl L, Cimatti L, Milanovic K, Jain M, Torgasheva N, Tanzer A, De Paepe K, Van De Wiele T, Hausmann B, Lang M, Pechhacker M, Ibrahim N, de Vries I, Brostjan C, Sixt MK, Gasche C, Boon L, Berry D, Jantsch MF, Pereira FC, Vesely C. 2025. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. Journal of Experimental Medicine. 222(9), e20240109.","mla":"Gawish, Riem, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9, e20240109, Rockefeller University Press, 2025, doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>."},"pmid":1,"type":"journal_article","quality_controlled":"1","publication_identifier":{"issn":["0022-1007"],"eissn":["1540-9538"]},"has_accepted_license":"1","issue":"9","article_number":"e20240109","volume":222,"file_date_updated":"2025-12-30T09:00:04Z","file":[{"file_id":"20899","relation":"main_file","checksum":"708d61fb8cf1d83ee1e33ddcfde0857e","file_name":"2025_JEM_Gawish.pdf","content_type":"application/pdf","creator":"dernst","success":1,"date_created":"2025-12-30T09:00:04Z","file_size":9349311,"access_level":"open_access","date_updated":"2025-12-30T09:00:04Z"}],"status":"public","acknowledgement":"Sequencing was performed by the Vienna BioCenter Core Facilities (Medical University of Vienna Core Facility) and the Biomedical Sequencing Facility at CeMM, Vienna. Cell sorting and flow cytometry were performed at the Core Facility Flow Cytometry and Imaging (Medical University of Vienna). We thank Jasmin Schwarz, Gudrun Kohl, Petra Pjevac, and Joana Seneca Silva from the Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna for assisting with amplicon and metagenomic sequencing, as well as repositing of sequencing data. We thank Sophia Derdak and Michael Schuster for initial data analysis, Robert Vilvoi and Stephan Hemm for animal handling, Marcel Kertesz for mouse genotyping, and Salwan Roumaia for next generation sequencing sample preparation. Treatment schemes and graphical abstracts were created with https://BioRender.com.\r\n\r\nThis work was supported by the Austrian Science Fund, grant number ZK 57-B28 to C. Vesely, R. Gawish, and F.C. Pereira; grant number V 1025-B to R. Gawish; grant number DOC32-B28 to R. Varada and M.F. Jantsch; and F8007 and P32678 to M.F. Jantsch. Open Access funding provided by Medical University of Vienna.","day":"01","year":"2025","date_published":"2025-09-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"article_processing_charge":"Yes (via OA deal)","_id":"19928","author":[{"first_name":"Riem","full_name":"Gawish, Riem","last_name":"Gawish"},{"last_name":"Varada","full_name":"Varada, Rajagopal","first_name":"Rajagopal"},{"last_name":"Deckert","full_name":"Deckert, Florian","first_name":"Florian"},{"first_name":"Anastasiya","full_name":"Hladik, Anastasiya","last_name":"Hladik"},{"first_name":"Linda","last_name":"Steinbichl","full_name":"Steinbichl, Linda"},{"first_name":"Laura","last_name":"Cimatti","full_name":"Cimatti, Laura"},{"first_name":"Katarina","last_name":"Milanovic","full_name":"Milanovic, Katarina"},{"last_name":"Jain","full_name":"Jain, Mamta","first_name":"Mamta"},{"first_name":"Natalya","last_name":"Torgasheva","full_name":"Torgasheva, Natalya"},{"first_name":"Andrea","last_name":"Tanzer","full_name":"Tanzer, Andrea"},{"last_name":"De Paepe","full_name":"De Paepe, Kim","first_name":"Kim"},{"first_name":"Tom","last_name":"Van De Wiele","full_name":"Van De Wiele, Tom"},{"full_name":"Hausmann, Bela","last_name":"Hausmann","first_name":"Bela"},{"last_name":"Lang","full_name":"Lang, Michaela","first_name":"Michaela"},{"first_name":"Martin","full_name":"Pechhacker, Martin","last_name":"Pechhacker"},{"last_name":"Ibrahim","full_name":"Ibrahim, Nahla","first_name":"Nahla"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid"},{"last_name":"Brostjan","full_name":"Brostjan, Christine","first_name":"Christine"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Christoph","full_name":"Gasche, Christoph","last_name":"Gasche"},{"first_name":"Louis","last_name":"Boon","full_name":"Boon, Louis"},{"full_name":"Berry, David","last_name":"Berry","first_name":"David"},{"last_name":"Jantsch","full_name":"Jantsch, Michael F.","first_name":"Michael F."},{"full_name":"Pereira, Fatima C.","last_name":"Pereira","first_name":"Fatima C."},{"first_name":"Cornelia","last_name":"Vesely","full_name":"Vesely, Cornelia"}],"publisher":"Rockefeller University Press","intvolume":"       222","oa":1,"publication":"Journal of Experimental Medicine","date_created":"2025-06-29T22:01:15Z","ddc":["570"],"isi":1,"month":"09","external_id":{"pmid":["40471139"],"isi":["001502896900001"]},"title":"Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis","abstract":[{"lang":"eng","text":"Patho-mechanistic origins of ulcerative colitis are still poorly understood. The actin cross-linker filamin A (FLNA) impacts cellular responses through interaction with cytosolic proteins. Posttranscriptional A-to-I editing generates two forms of FLNA: genome-encoded FLNAQ and FLNAR. FLNA is edited in colon fibroblasts, smooth muscle cells, and endothelial cells. We found that the FLNA editing status determines colitis severity. Editing was highest in healthy colons and reduced during murine and human colitis. Mice that exclusively express FLNAR were highly resistant to DSS-induced colitis, whereas fully FLNAQ animals developed severe inflammation. While the genetic induction of FLNA editing influenced transcriptional states of structural cells and microbiome composition, we found that FLNAR exerts protection specifically via myeloid cells, which are physiologically unedited. Introducing fixed FLNAR did not hamper cell migration but reduced macrophage inflammation and rendered neutrophils less prone to NETosis. Thus, loss of FLNA editing correlates with colitis severity, and targeted editing of myeloid cells serves as a novel therapeutic approach in intestinal inflammation."}],"doi":"10.1084/jem.20240109","OA_place":"publisher","oa_version":"Published Version","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2025-12-30T09:00:42Z","scopus_import":"1"},{"type":"journal_article","quality_controlled":"1","pmid":1,"citation":{"mla":"LI, ZIQIANG, and Michael K. Sixt. “Cell Migration: How Animal Cells Run and Tumble.” <i>Current Biology</i>, vol. 35, no. 18, Elsevier, 2025, pp. R890–92, doi:<a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">10.1016/j.cub.2025.08.016</a>.","ama":"LI Z, Sixt MK. Cell migration: How animal cells run and tumble. <i>Current Biology</i>. 2025;35(18):R890-R892. doi:<a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">10.1016/j.cub.2025.08.016</a>","ista":"LI Z, Sixt MK. 2025. Cell migration: How animal cells run and tumble. Current Biology. 35(18), R890–R892.","apa":"LI, Z., &#38; Sixt, M. K. (2025). Cell migration: How animal cells run and tumble. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">https://doi.org/10.1016/j.cub.2025.08.016</a>","ieee":"Z. LI and M. K. Sixt, “Cell migration: How animal cells run and tumble,” <i>Current Biology</i>, vol. 35, no. 18. Elsevier, pp. R890–R892, 2025.","short":"Z. LI, M.K. Sixt, Current Biology 35 (2025) R890–R892.","chicago":"LI, ZIQIANG, and Michael K Sixt. “Cell Migration: How Animal Cells Run and Tumble.” <i>Current Biology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">https://doi.org/10.1016/j.cub.2025.08.016</a>."},"article_type":"letter_note","OA_type":"closed access","issue":"18","publication_identifier":{"eissn":["1879-0445"]},"status":"public","volume":35,"publisher":"Elsevier","article_processing_charge":"No","_id":"20427","author":[{"id":"922e68bb-1727-11ee-857c-966e8cc1b6c3","first_name":"Ziqiang","full_name":"Li, Ziqiang","last_name":"Li"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"year":"2025","day":"22","date_published":"2025-09-22T00:00:00Z","intvolume":"        35","isi":1,"month":"09","corr_author":"1","publication":"Current Biology","page":"R890-R892","date_created":"2025-10-05T22:01:35Z","doi":"10.1016/j.cub.2025.08.016","title":"Cell migration: How animal cells run and tumble","abstract":[{"lang":"eng","text":"Animal cells migrating up chemotactic gradients often show speed oscillations. A new study describes a molecular circuit that switches zebrafish germ cells between phases of straight runs, tumbling and directional reorientation."}],"external_id":{"pmid":["40987270"],"isi":["001592664700001"]},"scopus_import":"1","date_updated":"2025-12-01T12:54:02Z","publication_status":"published","oa_version":"None","language":[{"iso":"eng"}]},{"status":"public","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","file":[{"content_type":"application/pdf","file_name":"2025_CellReports_Tavano.pdf","checksum":"57e05dd1598c807af0afdb32cec039d3","success":1,"creator":"dernst","file_id":"19413","relation":"main_file","access_level":"open_access","date_updated":"2025-03-17T10:26:54Z","file_size":9067797,"date_created":"2025-03-17T10:26:54Z"}],"file_date_updated":"2025-03-17T10:26:54Z","volume":44,"acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"date_published":"2025-03-25T00:00:00Z","day":"25","year":"2025","publisher":"Elsevier","_id":"19404","author":[{"id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","first_name":"Ste","orcid":"0000-0001-9970-7804","full_name":"Tavano, Ste","last_name":"Tavano"},{"last_name":"Brückner","full_name":"Brückner, David","orcid":"0000-0001-7205-2975","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d"},{"last_name":"Tasciyan","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren"},{"full_name":"Tong, Xin","last_name":"Tong","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin"},{"last_name":"Kardos","full_name":"Kardos, Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland"},{"full_name":"Schauer, Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","first_name":"Alexandra","orcid":"0000-0001-7659-9142"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"article_processing_charge":"Yes","citation":{"apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>.","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>."},"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"article_type":"original","OA_type":"gold","quality_controlled":"1","project":[{"name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022"},{"name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","_id":"269CD5C4-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1159-2018"}],"type":"journal_article","pmid":1,"has_accepted_license":"1","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"article_number":"115387","issue":"3","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"DOAJ_listed":"1","external_id":{"isi":["001443652700001"],"pmid":["40057955"]},"OA_place":"publisher","doi":"10.1016/j.celrep.2025.115387","language":[{"iso":"eng"}],"oa_version":"Published Version","scopus_import":"1","date_updated":"2025-10-22T07:00:04Z","publication_status":"published","intvolume":"        44","oa":1,"date_created":"2025-03-16T23:01:24Z","publication":"Cell Reports","month":"03","ddc":["570"],"isi":1,"corr_author":"1"},{"OA_place":"publisher","doi":"10.1111/jdv.20291","title":"Langerhans cells: Central players in the pathophysiology of atopic dermatitis","abstract":[{"text":"Atopic dermatitis (AD) is the most common chronic inflammatory skin disease worldwide. AD is a highly complex disease with different subtypes. Many elements of AD pathophysiology have been described, but if/how they interact with each other or which mechanisms are important in which patients is still unclear. Langerhans cells (LCs) are antigen-presenting cells (APCs) in the epidermis. Depending on the context, they can act either pro- or anti-inflammatory. Many different studies have investigated LCs in the context of AD and found them to be connected to all major mechanisms of AD pathophysiology. As APCs, LCs recruit other immune cells and shape the immune response, especially adaptive immunity via polarization of T cells. As sentinel cells, LCs are primary sensors of the skin microbiome and are important for the decision of immunity versus tolerance. LCs are also involved with the integrity of the skin barrier by influencing tight junctions. Finally, LCs are important cells in the neuro-immune crosstalk in the skin. In this review, we provide an overview about the many different roles of LCs in AD. Understanding LCs might bring us closer to a more complete understanding of this highly complex disease. Potentially, modulating LCs might offer new options for targeted therapies for AD patients.","lang":"eng"}],"external_id":{"isi":["001292894900001"],"pmid":["39157943"]},"scopus_import":"1","publication_status":"published","date_updated":"2025-05-19T13:58:50Z","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"intvolume":"        39","month":"02","isi":1,"ddc":["570"],"date_created":"2024-08-25T22:01:07Z","publication":"Journal of the European Academy of Dermatology and Venereology","page":"278-289","acknowledgement":"This work was supported by the CK-CARE of the KühneFoundation, Switzerland; the China Scholarship Counciland Shanghai Biocelline Enterprise Co. Ltd, China.","status":"public","file":[{"date_created":"2025-04-16T09:59:37Z","file_size":457698,"access_level":"open_access","date_updated":"2025-04-16T09:59:37Z","file_id":"19583","relation":"main_file","checksum":"12555ddb3490daf10b8d44e334e7312e","file_name":"2025_JEADV_Pan.pdf","content_type":"application/pdf","creator":"dernst","success":1}],"volume":39,"file_date_updated":"2025-04-16T09:59:37Z","publisher":"Wiley","_id":"17459","article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"Yi","last_name":"Pan","full_name":"Pan, Yi"},{"first_name":"Mathias","full_name":"Hochgerner, Mathias","last_name":"Hochgerner"},{"last_name":"Cichon","full_name":"Cichon, Malgorzata Anna","id":"d63197a3-c188-11ed-9387-8d33a3f13871","first_name":"Malgorzata Anna"},{"last_name":"Benezeder","full_name":"Benezeder, Theresa","first_name":"Theresa"},{"full_name":"Bieber, Thomas","last_name":"Bieber","first_name":"Thomas"},{"last_name":"Wolf","full_name":"Wolf, Peter","first_name":"Peter"}],"department":[{"_id":"MiSi"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-02-01T00:00:00Z","year":"2025","day":"01","quality_controlled":"1","type":"journal_article","pmid":1,"citation":{"ista":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. 2025. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. Journal of the European Academy of Dermatology and Venereology. 39(2), 278–289.","ama":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. <i>Journal of the European Academy of Dermatology and Venereology</i>. 2025;39(2):278-289. doi:<a href=\"https://doi.org/10.1111/jdv.20291\">10.1111/jdv.20291</a>","ieee":"Y. Pan, M. Hochgerner, M. A. Cichon, T. Benezeder, T. Bieber, and P. Wolf, “Langerhans cells: Central players in the pathophysiology of atopic dermatitis,” <i>Journal of the European Academy of Dermatology and Venereology</i>, vol. 39, no. 2. Wiley, pp. 278–289, 2025.","apa":"Pan, Y., Hochgerner, M., Cichon, M. A., Benezeder, T., Bieber, T., &#38; Wolf, P. (2025). Langerhans cells: Central players in the pathophysiology of atopic dermatitis. <i>Journal of the European Academy of Dermatology and Venereology</i>. Wiley. <a href=\"https://doi.org/10.1111/jdv.20291\">https://doi.org/10.1111/jdv.20291</a>","chicago":"Pan, Yi, Mathias Hochgerner, Malgorzata Anna Cichon, Theresa Benezeder, Thomas Bieber, and Peter Wolf. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” <i>Journal of the European Academy of Dermatology and Venereology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/jdv.20291\">https://doi.org/10.1111/jdv.20291</a>.","short":"Y. Pan, M. Hochgerner, M.A. Cichon, T. Benezeder, T. Bieber, P. Wolf, Journal of the European Academy of Dermatology and Venereology 39 (2025) 278–289.","mla":"Pan, Yi, et al. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” <i>Journal of the European Academy of Dermatology and Venereology</i>, vol. 39, no. 2, Wiley, 2025, pp. 278–89, doi:<a href=\"https://doi.org/10.1111/jdv.20291\">10.1111/jdv.20291</a>."},"OA_type":"hybrid","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","issue":"2","has_accepted_license":"1","publication_identifier":{"issn":["0926-9959"],"eissn":["1468-3083"]}},{"oa":1,"corr_author":"1","month":"09","ddc":["539","570"],"date_created":"2026-03-11T08:40:06Z","OA_place":"repository","doi":"10.1101/2025.05.20.655037","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"date_updated":"2026-03-18T14:11:35Z","publication_status":"draft","language":[{"iso":"eng"}],"oa_version":"Preprint","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"grant_number":"26360","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","name":"Motile active matter models of migrating cells and chiral filaments"}],"type":"preprint","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"citation":{"ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” bioRxiv, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, (n.d.).","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening.” bioRxiv.","mla":"Dunajova, Zuzana, et al. <i>Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening</i>. bioRxiv, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>."},"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"21423"},{"id":"21439","relation":"research_data","status":"public"}]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.20.655037"}],"has_accepted_license":"1","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","status":"public","article_processing_charge":"No","_id":"21427","author":[{"full_name":"Dunajova, Zuzana","last_name":"Dunajova","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","first_name":"Zuzana"},{"full_name":"Tasciyan, Saren","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","orcid":"0000-0003-1671-393X"},{"id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","first_name":"Juraj","full_name":"Majek, Juraj","last_name":"Majek"},{"last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack"},{"full_name":"Sahai, Erik","last_name":"Sahai","first_name":"Erik"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"}],"publisher":"bioRxiv","date_published":"2025-09-25T00:00:00Z","year":"2025","day":"25","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}]},{"related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/bench-pressing-cells/","description":"News on ISTA website"}],"record":[{"relation":"dissertation_contains","status":"public","id":"20149"}]},"has_accepted_license":"1","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"PlanS_conform":"1","quality_controlled":"1","project":[{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"},{"grant_number":"944-2020","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration"}],"type":"journal_article","pmid":1,"citation":{"ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"letter_note","OA_type":"hybrid","publisher":"Springer Nature","_id":"20082","author":[{"full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","first_name":"Patricia","orcid":"0000-0003-1681-508X"},{"full_name":"Avellaneda Sarrió, Mario","last_name":"Avellaneda Sarrió","first_name":"Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","orcid":"0000-0001-6406-524X"},{"full_name":"Canigova, Nikola","last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87","first_name":"Nikola","orcid":"0000-0002-8518-5926"},{"orcid":"0000-0001-6120-3723","first_name":"Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","full_name":"Gärtner, Florian R"},{"full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518"},{"last_name":"Riedl","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","first_name":"Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid","last_name":"De Vries"},{"full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"full_name":"Fukui, Yoshinori","last_name":"Fukui","first_name":"Yoshinori"},{"full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia","first_name":"Alba","id":"40F05888-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1009-9652"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K"}],"article_processing_charge":"Yes (via OA deal)","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"date_published":"2025-08-01T00:00:00Z","day":"01","year":"2025","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","status":"public","file":[{"date_updated":"2025-07-31T08:00:33Z","access_level":"open_access","date_created":"2025-07-31T08:00:33Z","file_size":13514646,"success":1,"creator":"dernst","file_name":"2025_NatureImmunology_ReisRodrigues.pdf","checksum":"0c725123dca7797c682609bff2c4c5ac","content_type":"application/pdf","relation":"main_file","file_id":"20096"}],"file_date_updated":"2025-07-31T08:00:33Z","volume":26,"month":"08","isi":1,"ddc":["570"],"corr_author":"1","date_created":"2025-07-27T22:01:26Z","publication":"Nature Immunology","page":"1258–1266","oa":1,"intvolume":"        26","scopus_import":"1","publication_status":"published","date_updated":"2026-04-28T13:26:50Z","language":[{"iso":"eng"}],"oa_version":"Published Version","OA_place":"publisher","doi":"10.1038/s41590-025-02211-w","abstract":[{"lang":"eng","text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Migrating immune cells globally coordinate protrusive forces","external_id":{"isi":["001529134300001"],"pmid":["40664976"]}},{"supervisor":[{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"corr_author":"1","month":"08","ddc":["570"],"date_created":"2025-08-08T09:18:02Z","page":"114","oa":1,"date_updated":"2026-04-28T13:26:50Z","publication_status":"published","language":[{"iso":"eng"}],"oa_version":"Published Version","OA_place":"publisher","doi":"10.15479/AT-ISTA-20149","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"NanoFab"}],"title":"Coordination of protrusive forces in immune cell migration ","abstract":[{"lang":"eng","text":"Immune responses depend on the coordinated and efficient migration of leukocytes. These\r\ncells, which are embedded and tightly confined within tissues, must navigate and traverse\r\ndiverse and complex three-dimensional environments. Leukocytes adapt their locomotory\r\nbehavior to the mechanical, geometrical, and biochemical characteristics of their\r\nsurroundings. In low-density environments, where the pore size of the interstitial matrix\r\nallows free passage, these cells position the nucleus directly behind the lamellipodium, the\r\nprotrusive actin structure that forms the leading front of the cell. In this configuration, they\r\nuse the nucleus as a gauge to identify the path of least resistance.\r\nHere, we show that in high-density environments, where the pore size precludes free passage\r\nof the cell body, leukocytes reposition the microtubule-organizing center (MTOC) and\r\nassociated organelles in front of the nucleus. In this configuration, they use actin structures\r\nprotruding orthogonally to the direction of migration in order to open a path for the cell body.\r\nWe identify two distinct actin populations that serve this purpose at different subcellular\r\nlocalizations. At the leading edge, local indentation of the plasma membrane leads to\r\nrecruitment of the Wiskott-Aldrich syndrome protein (WASp), which, via Arp2/3, results in\r\nthe formation of individual actin foci. At the cell body, actin polymerization is triggered by\r\nDOCK8, a Cdc42 exchange factor, resulting in the formation of a central actin pool.\r\nWe demonstrate that the central and peripheral actin pools are functionally communicating\r\nand that depletion of the central actin pool leads to increased actin accumulation at the cell\r\nfront, resulting in excessive extension of the leading edge."}],"degree_awarded":"PhD","related_material":{"record":[{"id":"10703","status":"public","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","status":"public","id":"20082"}]},"has_accepted_license":"1","publication_identifier":{"issn":["2663-337X"]},"project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"}],"type":"dissertation","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"citation":{"ista":"Dos Reis Rodrigues P. 2025. Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria.","ama":"Dos Reis Rodrigues P. Coordination of protrusive forces in immune cell migration . 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>","apa":"Dos Reis Rodrigues, P. (2025). <i>Coordination of protrusive forces in immune cell migration </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>","ieee":"P. Dos Reis Rodrigues, “Coordination of protrusive forces in immune cell migration ,” Institute of Science and Technology Austria, 2025.","chicago":"Dos Reis Rodrigues, Patricia. “Coordination of Protrusive Forces in Immune Cell Migration .” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>.","short":"P. Dos Reis Rodrigues, Coordination of Protrusive Forces in Immune Cell Migration , Institute of Science and Technology Austria, 2025.","mla":"Dos Reis Rodrigues, Patricia. <i>Coordination of Protrusive Forces in Immune Cell Migration </i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>."},"_id":"20149","article_processing_charge":"No","author":[{"last_name":"Dos Reis Rodrigues","full_name":"Dos Reis Rodrigues, Patricia","orcid":"0000-0003-1681-508X","first_name":"Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F"}],"alternative_title":["ISTA Thesis"],"publisher":"Institute of Science and Technology Austria","date_published":"2025-08-08T00:00:00Z","year":"2025","day":"08","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"acknowledgement":"I would like to acknowledge the\r\nfinancial support of the European Research Council through the ERC-SyG grant “Pushing from\r\nwithin: Control of cell shape, integrity and motility by cytoskeletal pushing forces”\r\n(01071793), which made this research possible. ","file_date_updated":"2025-08-27T13:02:28Z","status":"public","file":[{"file_size":63885565,"date_created":"2025-08-27T12:59:10Z","access_level":"open_access","date_updated":"2025-08-27T12:59:10Z","file_id":"20232","relation":"main_file","checksum":"fda8a1070667c3562263f4867609b41b","content_type":"application/pdf","file_name":"2025_ReisRodrigues_Patricia_Thesis.pdf","creator":"prodrigu","success":1},{"file_size":50483434,"date_created":"2025-08-27T13:00:30Z","access_level":"closed","date_updated":"2025-08-27T13:02:28Z","file_id":"20233","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"e8b65affcbce846a926454df4b2867b9","file_name":"2025_ReisRodrigues_Patricia_Thesis.docx","creator":"prodrigu"}]},{"oa":1,"intvolume":"       122","isi":1,"ddc":["570"],"month":"08","corr_author":"1","publication":"Proceedings of the National Academy of Sciences","date_created":"2025-09-07T22:01:32Z","doi":"10.1073/pnas.2504064122","OA_place":"publisher","abstract":[{"text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"title":"Self-generated chemotaxis of mixed cell populations","external_id":{"pmid":["40838890"],"isi":["001562181600001"]},"scopus_import":"1","APC_amount":"5766,07 EUR","date_updated":"2026-05-20T08:59:54Z","publication_status":"published","oa_version":"Published Version","language":[{"iso":"eng"}],"type":"journal_article","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"quality_controlled":"1","pmid":1,"citation":{"short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>.","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., &#38; Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34. National Academy of Sciences, 2025.","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(34). doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>."},"OA_type":"hybrid","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","issue":"34","article_number":"e2504064122","related_material":{"link":[{"url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis","relation":"software"}]},"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"has_accepted_license":"1","PlanS_conform":"1","acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","file":[{"date_created":"2025-09-08T07:23:29Z","file_size":16069140,"access_level":"open_access","date_updated":"2025-09-08T07:23:29Z","file_id":"20307","relation":"main_file","content_type":"application/pdf","checksum":"b36abd92673b6d76376fc9434bad52cc","file_name":"2025_PNAS_Ucar.pdf","success":1,"creator":"dernst"}],"ec_funded":1,"status":"public","volume":122,"file_date_updated":"2025-09-08T07:23:29Z","publisher":"National Academy of Sciences","_id":"20289","article_processing_charge":"Yes (in subscription journal)","author":[{"orcid":"0000-0003-0506-4217","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","last_name":"Ucar","full_name":"Ucar, Mehmet C"},{"last_name":"Zane","full_name":"Zane, Alsberga","orcid":"0009-0003-0415-7603","first_name":"Alsberga","id":"60f7509a-f652-11ea-9d86-b963d6490d7c"},{"full_name":"Alanko, Jonna H","last_name":"Alanko","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7698-3061"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"department":[{"_id":"EdHa"},{"_id":"MiSi"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","day":"26","date_published":"2025-08-26T00:00:00Z"},{"language":[{"iso":"eng"}],"oa_version":"Published Version","publication_status":"published","date_updated":"2026-04-07T12:38:44Z","abstract":[{"lang":"eng","text":"Cell migration is a crucial process in animal development and maintenance. It is incredibly\r\nheterogeneous, with different cell types utilizing fundamentally distinct migration strategies.\r\nThe strategies also depend on the cellular microenvironment, where cells can switch between\r\nmigration modes as they encounter new environmental cues. In this thesis, we investigated\r\nhow dendritic cells adapt their migration strategy when encountering geometrically,\r\nmechanically and chemically distinct environments.\r\nWhen dendritic cells are embedded in a homogeneous fibrous network, they migrate in a fast\r\nand directional amoeboid manner. In this migration strategy, extracellular proteolysis and\r\nintegrin-mediated adhesions are dispensable. Instead, the cells use topography of the\r\nenvironment to propel their cell body forward. To migrate efficiently in the maze of different\r\npore sizes, they position the nucleus ahead of the microtubule organizing center (MTOC) and\r\nuse it to gauge the pores to identify the path of least resistance. Our aim was to identify\r\nwhether dendritic cells adapt their migration strategy when encountering asymmetrical\r\ntransitions into much denser environments with limited choice of large pores. In such invasive\r\ntransitions it is unclear if the cells can cross tight pores without the use of adhesions and\r\nextracellular proteolysis and whether they maintain the nucleus in the cell front.\r\nUsing various cell migration assays such as fibrous 3D collagen gels, geometrically defined\r\nmicrochannels with constrictions and simplistic under agarose migration assay, we provide\r\na comprehensive characterization of invasive migration of dendritic cells. We show that\r\nduring invasion the cells stall and stretch, reflecting the difficulty to translocate the bulky cell\r\nbody into the dense environment. In collagen gels, we show that dendritic cells can invade\r\nwithout proteolysis and adhesions. Instead, they utilize contractility, which can lead to largescale collagen compressions. During invasion, the nucleus stalls at tight constrictions, leading\r\nto a transient organelle reorientation. To resolve the stalling, upregulated rear contractility is\r\nrequired. This contractile force is simultaneously necessary for reverting the nucleus back to\r\nthe cell front after invasion and maintaining this positioning during permissive migration.\r\nA functional role of the reorientation was uncovered in the first collaboration project.\r\nA prominent central actin pool was identified around the MTOC, especially pronounced in\r\ndense and compressive environments. The actin pool was shown to generate pushing forces\r\nto dilate the space for cell translocation. These forces are only necessary in non-permissive\r\nenvironments, where the nucleus reorients to the cell rear, allowing the actin pool to\r\ngenerate space. In permissive environments where space generation is dispensable, the\r\nMTOC is located behind the nucleus and the actin cloud has reduced intensity, allowing more\r\nactin to be incorporated into the lamellipodium, speeding up migration.\r\nIn the second collaboration project, we investigated the effects of distinct chemical\r\nenvironments on dendritic cell migration. The strikingly persistent migration of these cells\r\nwas explained by their ability to modulate and even self-generate chemokine gradients. This\r\nallows the cells to migrate faster and more persistent in uniform chemokine fields compared\r\nto imposed chemokine gradients. The chemokine receptor CCR7 was identified as a crucial\r\nplayer in this process, both sensing the signal and internalizing the chemokine to create a sink."}],"title":"Adaptive strategies of dendritic cell migration in response to environmental cues","degree_awarded":"PhD","OA_place":"publisher","doi":"10.15479/AT-ISTA-19745","date_created":"2025-05-26T08:49:00Z","page":"133","month":"05","ddc":["570"],"supervisor":[{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179"}],"corr_author":"1","oa":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","department":[{"_id":"MiSi"},{"_id":"GradSch"}],"date_published":"2025-05-27T00:00:00Z","day":"27","year":"2025","alternative_title":["ISTA Thesis"],"publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","_id":"19745","author":[{"orcid":"0000-0002-8518-5926","id":"3795523E-F248-11E8-B48F-1D18A9856A87","first_name":"Nikola","last_name":"Canigova","full_name":"Canigova, Nikola"}],"ec_funded":1,"status":"public","file":[{"file_name":"NikolaCanigova_Thesis_final.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"1a2d1525d19347fbb879ef57c02951bf","creator":"cchlebak","file_id":"19748","embargo_to":"open_access","relation":"source_file","access_level":"closed","date_updated":"2025-11-27T23:30:02Z","file_size":103879193,"date_created":"2025-05-28T07:38:17Z"},{"date_updated":"2025-11-27T23:30:02Z","access_level":"open_access","date_created":"2025-05-28T07:39:53Z","file_size":194530600,"creator":"cchlebak","content_type":"application/pdf","checksum":"c1d8f9a40a8e19fcf895373f4b773a46","file_name":"NikolaCanigova_Thesis_final_PDFA2a_fixed.pdf","relation":"main_file","file_id":"19749","embargo":"2025-11-27"}],"file_date_updated":"2025-11-27T23:30:02Z","acknowledgement":"This project has received funding from the Austrian Science Fund (FWF) via the doctorate\r\ncollege DK NanoCell and from the European Union’s Horizon 2020 research and innovation\r\nprogramme under the Marie Skłodowska-Curie Grant Agreement No. 665385.\r\n","has_accepted_license":"1","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-058-9"]},"OA_embargo":"6","related_material":{"record":[{"id":"14274","status":"public","relation":"part_of_dissertation"}]},"citation":{"apa":"Canigova, N. (2025). <i>Adaptive strategies of dendritic cell migration in response to environmental cues</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19745\">https://doi.org/10.15479/AT-ISTA-19745</a>","ieee":"N. Canigova, “Adaptive strategies of dendritic cell migration in response to environmental cues,” Institute of Science and Technology Austria, 2025.","chicago":"Canigova, Nikola. “Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19745\">https://doi.org/10.15479/AT-ISTA-19745</a>.","short":"N. Canigova, Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues, Institute of Science and Technology Austria, 2025.","ista":"Canigova N. 2025. Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria.","ama":"Canigova N. Adaptive strategies of dendritic cell migration in response to environmental cues. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19745\">10.15479/AT-ISTA-19745</a>","mla":"Canigova, Nikola. <i>Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19745\">10.15479/AT-ISTA-19745</a>."},"project":[{"grant_number":"665385","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program"},{"call_identifier":"FWF","_id":"265E2996-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W01250-B20"}],"type":"dissertation"},{"language":[{"iso":"eng"}],"oa_version":"Published Version","scopus_import":"1","date_updated":"2025-09-08T09:50:13Z","publication_status":"published","title":"Antibodies and complement are key drivers of thrombosis","abstract":[{"text":"Venous thromboembolism (VTE) is a common, deadly disease with an increasing incidence despite preventive efforts. Clinical observations have associated elevated antibody concentrations or antibody-based therapies with thrombotic events. However, how antibodies contribute to thrombosis is unknown. Here, we show that reduced blood flow enabled immunoglobulin M (IgM) to bind to FcμR and the polymeric immunoglobulin receptor (pIgR), initiating endothelial activation and platelet recruitment. Subsequently, the procoagulant surface of activated platelets accommodated antigen- and FcγR-independent IgG deposition. This leads to classical complement activation, setting in motion a prothrombotic vicious circle. Key elements of this mechanism were present in humans in the setting of venous stasis as well as in the dysregulated immunothrombosis of COVID-19. This antibody-driven thrombosis can be prevented by pharmacologically targeting complement. Hence, our results uncover antibodies as previously unrecognized central regulators of thrombosis. These findings carry relevance for therapeutic application of antibodies and open innovative avenues to target thrombosis without compromising hemostasis.","lang":"eng"}],"external_id":{"isi":["001317438500001"],"pmid":["39226900"]},"doi":"10.1016/j.immuni.2024.08.007","date_created":"2024-09-22T22:01:42Z","publication":"Immunity","page":"2140-2156","month":"09","ddc":["570"],"isi":1,"intvolume":"        57","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"date_published":"2024-09-10T00:00:00Z","day":"10","year":"2024","publisher":"Elsevier","_id":"18109","author":[{"last_name":"Stark","full_name":"Stark, Konstantin","first_name":"Konstantin"},{"last_name":"Kilani","full_name":"Kilani, Badr","first_name":"Badr"},{"last_name":"Stockhausen","full_name":"Stockhausen, Sven","first_name":"Sven"},{"full_name":"Busse, Johanna","last_name":"Busse","first_name":"Johanna"},{"full_name":"Schubert, Irene","last_name":"Schubert","first_name":"Irene"},{"full_name":"Tran, Thuy Duong","last_name":"Tran","first_name":"Thuy Duong"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","first_name":"Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"first_name":"Kami","last_name":"Pekayvaz","full_name":"Pekayvaz, Kami"},{"first_name":"Leo","last_name":"Nicolai","full_name":"Nicolai, Leo"},{"first_name":"Valeria","full_name":"Fumagalli, Valeria","last_name":"Fumagalli"},{"first_name":"Julia","last_name":"Stermann","full_name":"Stermann, Julia"},{"full_name":"Stephan, Felix","last_name":"Stephan","first_name":"Felix"},{"last_name":"David","full_name":"David, Christian","first_name":"Christian"},{"full_name":"Müller, Martin B.","last_name":"Müller","first_name":"Martin B."},{"full_name":"Heyman, Birgitta","last_name":"Heyman","first_name":"Birgitta"},{"first_name":"Anja","full_name":"Lux, Anja","last_name":"Lux"},{"full_name":"Da Palma Guerreiro, Alexandra","last_name":"Da Palma Guerreiro","first_name":"Alexandra"},{"first_name":"Lukas P.","full_name":"Frenzel, Lukas P.","last_name":"Frenzel"},{"last_name":"Schmidt","full_name":"Schmidt, Christoph Q.","first_name":"Christoph Q."},{"last_name":"Dopler","full_name":"Dopler, Arthur","first_name":"Arthur"},{"first_name":"Markus","full_name":"Moser, Markus","last_name":"Moser"},{"last_name":"Chandraratne","full_name":"Chandraratne, Sue","first_name":"Sue"},{"first_name":"Marie Luise","full_name":"Von Brühl, Marie Luise","last_name":"Von Brühl"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"first_name":"Thomas","last_name":"Korff","full_name":"Korff, Thomas"},{"first_name":"Martina","last_name":"Rudelius","full_name":"Rudelius, Martina"},{"first_name":"Oliver","full_name":"Popp, Oliver","last_name":"Popp"},{"full_name":"Kirchner, Marieluise","last_name":"Kirchner","first_name":"Marieluise"},{"full_name":"Mertins, Philipp","last_name":"Mertins","first_name":"Philipp"},{"full_name":"Nimmerjahn, Falk","last_name":"Nimmerjahn","first_name":"Falk"},{"first_name":"Matteo","full_name":"Iannacone, Matteo","last_name":"Iannacone"},{"first_name":"Markus","full_name":"Sperandio, Markus","last_name":"Sperandio"},{"first_name":"Bernd","last_name":"Engelmann","full_name":"Engelmann, Bernd"},{"first_name":"Admar","last_name":"Verschoor","full_name":"Verschoor, Admar"},{"full_name":"Massberg, Steffen","last_name":"Massberg","first_name":"Steffen"}],"article_processing_charge":"Yes (in subscription journal)","status":"public","file":[{"content_type":"application/pdf","file_name":"2024_Immunity_Stark.pdf","checksum":"4683de43d06a8fd8e3fc91af4ddc1ba2","creator":"dernst","success":1,"file_id":"18162","relation":"main_file","access_level":"open_access","date_updated":"2024-09-30T09:16:03Z","file_size":6892750,"date_created":"2024-09-30T09:16:03Z"}],"file_date_updated":"2024-09-30T09:16:03Z","volume":57,"acknowledgement":"We thank Michael Carroll (Harvard Medical School, Boston) for providing Ighmtm1Che, C4−/−, and C3−/− mice; Mark Suter (University of Zurich, Zurich) for providing Aicda−/− mice; Marina Botto (Imperial College London, London) for providing C1q−/− and fB−/− mice; Craig Gerard (Harvard Medical School, Boston) for providing C3aR−/− mice; Falk Nimmerjahn (University Erlangen-Nuernberg, Erlangen) for providing Fcgr−/−Fcgr2b−/− mice; Karl Lang (University of Duisburg-Essen, Essen) for providing Fcmr−/− mice; Hans Hengartner and Rolf Zinkernagel (ETH Zurich, Zurich) for providing KL25 mice; Mark Zabel (University Hospital of Zurich, Zurich) for providing CR2−/− mice; Christie Ballantyne (Baylor College of Medicine, Houston) for providing CD11c−/− mice; and Siamon Gordon (University of Oxford, Oxford) for providing CD11b−/− mice. A.V. wishes to thank Michael Grünaug and dedicates this work to Annette, Rita, and Hans.\r\nThis project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. \r\n947611) (K.S.). This study was supported by the Deutsche Forschungsgemeinschaft through the collaborative research center 914 project B02 (K.S. and S.M.), project B04 (A.V.), project A01 (M.M.), project B01 (M.S.), the collaborative research center 1123 project B07 (K.S. and S.M.), the collaborative research center 359 (project A03 [K.S.] and B02 [M.S.]), the international research training group 1911 project B09 (A.V.), the clinical research unit 303 project 7 (A.V.), cluster of excellence 2167 (A.V.), collaborative research center 1526 project 05 (A.V.), the ANR-DFG project JAKPOT (K.S.), LMUexcellent (K.S.), and the Deutsche Zentrum für Herz-Kreislauf-Forschung (PostDoc Grant and partner site project [K.S. and S.M.]). M.I. is supported by the European Research Council (ERC) Advanced Grant 101141363, ERC Proof of Concept Grant 101138728, Italian Association for Cancer Research (AIRC) Grants 19891 and \r\n22737, Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and PRIN \r\n2022FMESXL, Funded Research Agreement from Asher Biotherapeutics, VIR Biotechnology, and BlueJay Therapeutics. V.F. is supported by the Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and Fondazione Prossimo Mio.","has_accepted_license":"1","publication_identifier":{"eissn":["1097-4180"]},"issue":"9","citation":{"ama":"Stark K, Kilani B, Stockhausen S, et al. Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. 2024;57(9):2140-2156. doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>","ista":"Stark K, Kilani B, Stockhausen S, Busse J, Schubert I, Tran TD, Gärtner FR, Leunig A, Pekayvaz K, Nicolai L, Fumagalli V, Stermann J, Stephan F, David C, Müller MB, Heyman B, Lux A, Da Palma Guerreiro A, Frenzel LP, Schmidt CQ, Dopler A, Moser M, Chandraratne S, Von Brühl ML, Lorenz M, Korff T, Rudelius M, Popp O, Kirchner M, Mertins P, Nimmerjahn F, Iannacone M, Sperandio M, Engelmann B, Verschoor A, Massberg S. 2024. Antibodies and complement are key drivers of thrombosis. Immunity. 57(9), 2140–2156.","apa":"Stark, K., Kilani, B., Stockhausen, S., Busse, J., Schubert, I., Tran, T. D., … Massberg, S. (2024). Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>","ieee":"K. Stark <i>et al.</i>, “Antibodies and complement are key drivers of thrombosis,” <i>Immunity</i>, vol. 57, no. 9. Elsevier, pp. 2140–2156, 2024.","chicago":"Stark, Konstantin, Badr Kilani, Sven Stockhausen, Johanna Busse, Irene Schubert, Thuy Duong Tran, Florian R Gärtner, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>.","short":"K. Stark, B. Kilani, S. Stockhausen, J. Busse, I. Schubert, T.D. Tran, F.R. Gärtner, A. Leunig, K. Pekayvaz, L. Nicolai, V. Fumagalli, J. Stermann, F. Stephan, C. David, M.B. Müller, B. Heyman, A. Lux, A. Da Palma Guerreiro, L.P. Frenzel, C.Q. Schmidt, A. Dopler, M. Moser, S. Chandraratne, M.L. Von Brühl, M. Lorenz, T. Korff, M. Rudelius, O. Popp, M. Kirchner, P. Mertins, F. Nimmerjahn, M. Iannacone, M. Sperandio, B. Engelmann, A. Verschoor, S. Massberg, Immunity 57 (2024) 2140–2156.","mla":"Stark, Konstantin, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>, vol. 57, no. 9, Elsevier, 2024, pp. 2140–56, doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>."},"article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"quality_controlled":"1","type":"journal_article","pmid":1},{"intvolume":"        20","oa":1,"date_created":"2024-01-21T23:00:57Z","page":"310-321","publication":"Nature Physics","corr_author":"1","month":"02","ddc":["530"],"isi":1,"external_id":{"pmid":["38370025"],"isi":["001138880800005"]},"title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","abstract":[{"text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces.","lang":"eng"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"doi":"10.1038/s41567-023-02302-1","language":[{"iso":"eng"}],"oa_version":"Published Version","date_updated":"2025-09-04T11:48:28Z","publication_status":"published","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","citation":{"mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321."},"pmid":1,"quality_controlled":"1","project":[{"grant_number":"I03601","call_identifier":"FWF","name":"Control of embryonic cleavage pattern","_id":"2646861A-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","has_accepted_license":"1","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"related_material":{"link":[{"url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","description":"News on ISTA Website","relation":"press_release"}]},"file_date_updated":"2024-07-16T12:12:43Z","volume":20,"status":"public","file":[{"date_created":"2024-07-16T12:12:43Z","file_size":9897883,"date_updated":"2024-07-16T12:12:43Z","access_level":"open_access","relation":"main_file","file_id":"17267","creator":"dernst","success":1,"content_type":"application/pdf","checksum":"7891ebe7c900ae47469ab127031dd1ec","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf"}],"acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","date_published":"2024-02-01T00:00:00Z","day":"01","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"_id":"14846","author":[{"last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia","orcid":"0000-0002-5223-3346","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia"},{"last_name":"Shinde","full_name":"Shinde, Rushikesh","first_name":"Rushikesh"},{"id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","first_name":"Madison","orcid":"0000-0002-8176-4824","full_name":"Bolger-Munro, Madison","last_name":"Bolger-Munro"},{"orcid":"0000-0002-3415-4628","first_name":"Matilda","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","last_name":"Peruzzo","full_name":"Peruzzo, Matilda"},{"last_name":"Szep","full_name":"Szep, Gregory","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory"},{"last_name":"Steccari","full_name":"Steccari, Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene"},{"last_name":"Labrousse Arias","full_name":"Labrousse Arias, David","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","first_name":"David"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa","last_name":"Zheden"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin"},{"full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones","first_name":"Andrew"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"article_processing_charge":"Yes (in subscription journal)","publisher":"Springer Nature"},{"issue":"1","has_accepted_license":"1","publication_identifier":{"eissn":["1469-3178"]},"quality_controlled":"1","type":"journal_article","pmid":1,"citation":{"ieee":"A. Pimenta-Marques <i>et al.</i>, “Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM,” <i>EMBO Reports</i>, vol. 25, no. 1. Embo Press, pp. 102–127, 2024.","apa":"Pimenta-Marques, A., Perestrelo, T., Dos Reis Rodrigues, P., Duarte, P., Ferreira-Silva, A., Lince-Faria, M., &#38; Bettencourt-Dias, M. (2024). Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. <i>EMBO Reports</i>. Embo Press. <a href=\"https://doi.org/10.1038/s44319-023-00020-6\">https://doi.org/10.1038/s44319-023-00020-6</a>","chicago":"Pimenta-Marques, Ana, Tania Perestrelo, Patricia Dos Reis Rodrigues, Paulo Duarte, Ana Ferreira-Silva, Mariana Lince-Faria, and Mónica Bettencourt-Dias. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” <i>EMBO Reports</i>. Embo Press, 2024. <a href=\"https://doi.org/10.1038/s44319-023-00020-6\">https://doi.org/10.1038/s44319-023-00020-6</a>.","short":"A. Pimenta-Marques, T. Perestrelo, P. Dos Reis Rodrigues, P. Duarte, A. Ferreira-Silva, M. Lince-Faria, M. Bettencourt-Dias, EMBO Reports 25 (2024) 102–127.","ama":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, et al. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. <i>EMBO Reports</i>. 2024;25(1):102-127. doi:<a href=\"https://doi.org/10.1038/s44319-023-00020-6\">10.1038/s44319-023-00020-6</a>","ista":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, Duarte P, Ferreira-Silva A, Lince-Faria M, Bettencourt-Dias M. 2024. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. EMBO Reports. 25(1), 102–127.","mla":"Pimenta-Marques, Ana, et al. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” <i>EMBO Reports</i>, vol. 25, no. 1, Embo Press, 2024, pp. 102–27, doi:<a href=\"https://doi.org/10.1038/s44319-023-00020-6\">10.1038/s44319-023-00020-6</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","publisher":"Embo Press","_id":"14933","author":[{"first_name":"Ana","full_name":"Pimenta-Marques, Ana","last_name":"Pimenta-Marques"},{"first_name":"Tania","last_name":"Perestrelo","full_name":"Perestrelo, Tania"},{"full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","first_name":"Patricia","orcid":"0000-0003-1681-508X"},{"first_name":"Paulo","full_name":"Duarte, Paulo","last_name":"Duarte"},{"first_name":"Ana","last_name":"Ferreira-Silva","full_name":"Ferreira-Silva, Ana"},{"first_name":"Mariana","full_name":"Lince-Faria, Mariana","last_name":"Lince-Faria"},{"first_name":"Mónica","last_name":"Bettencourt-Dias","full_name":"Bettencourt-Dias, Mónica"}],"article_processing_charge":"Yes (in subscription journal)","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"date_published":"2024-01-10T00:00:00Z","day":"10","year":"2024","acknowledgement":"We thank all members of the Cell Cycle and Regulation Lab for the discussions and for the critical reading of the manuscript. We thank Tomer Avidor-Reiss (University of Toledo, Toledo, OH), Daniel St. Johnston (The Gurdon Institute, Cambridge, UK), David Glover (University of Cambridge, Cambridge, UK), Jingyan Fu (Agricultural University, Beijing, China) Jordan Raff (University of Oxford, Oxford, UK) and Timothy Megraw (Florida State University, Tallahassee, FL) for sharing tools. We acknowledge the technical support of Instituto Gulbenkian de Ciência (IGC)‘s Advanced Imaging Facility, in particular Gabriel Martins, Nuno Pimpão Martins and José Marques. We also thank Tiago Paixão from the IGC’s Quantitative & Digital Science Unit and Marco Louro from the CCR lab for the support provided on statistical analysis. IGC’s Advanced Imaging Facility (AIF-UIC) is supported by the national Portuguese funding ref# PPBI-POCI-01-0145-FEDER -022122. We thank the IGC’s Fly Facility, supported by CONGENTO (LISBOA-01-0145-FEDER-022170). This work was supported by an ERC grant (ERC-2015-CoG-683258) awarded to MBD and a grant from the Portuguese Research Council (FCT) awarded to APM (PTDC/BIA-BID/32225/2017).","status":"public","file":[{"file_id":"14941","relation":"main_file","checksum":"53c3ef43d9bd6d7bff3ffcf57d763cac","file_name":"2023_EmboReports_PimentaMarques.pdf","content_type":"application/pdf","success":1,"creator":"dernst","file_size":9645056,"date_created":"2024-02-05T12:35:03Z","access_level":"open_access","date_updated":"2024-02-05T12:35:03Z"}],"file_date_updated":"2024-02-05T12:35:03Z","volume":25,"month":"01","ddc":["570"],"date_created":"2024-02-04T23:00:53Z","page":"102-127","publication":"EMBO Reports","oa":1,"intvolume":"        25","scopus_import":"1","publication_status":"published","date_updated":"2025-04-23T07:39:52Z","language":[{"iso":"eng"}],"oa_version":"Published Version","doi":"10.1038/s44319-023-00020-6","title":"Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM","abstract":[{"lang":"eng","text":"Centrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, are downregulated in oogenesis and sufficient for maintaining both centrosome structural integrity and MTOC activity. We now show that the expression of specific components of the centriole cartwheel and wall, including ANA1/CEP295, is essential for maintaining centrosome integrity. We find that Polo kinase requires ANA1 to promote centriole stability in cultured cells and eggs. In addition, ANA1 expression prevents the loss of centrioles observed upon PCM-downregulation. However, the centrioles maintained by overexpressing and tethering ANA1 are inactive, unlike the MTOCs observed upon tethering Polo kinase. These findings demonstrate that several centriole components are needed to maintain centrosome structure. Our study also highlights that centrioles are more dynamic than previously believed, with their structural stability relying on the continuous expression of multiple components."}],"external_id":{"pmid":["38200359"]}},{"publisher":"Rockefeller University Press","article_processing_charge":"Yes (via OA deal)","_id":"15146","author":[{"first_name":"Bettina","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9561-1239","full_name":"Zens, Bettina","last_name":"Zens"},{"first_name":"Florian","id":"404F5528-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7149-769X","full_name":"Fäßler, Florian","last_name":"Fäßler"},{"full_name":"Hansen, Jesse","last_name":"Hansen","first_name":"Jesse","id":"1063c618-6f9b-11ec-9123-f912fccded63","orcid":"0000-0001-7967-2085"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert"},{"orcid":"0000-0002-3616-8580","first_name":"Julia","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","last_name":"Datler","full_name":"Datler, Julia"},{"last_name":"Hodirnau","full_name":"Hodirnau, Victor-Valentin","orcid":"0000-0003-3904-947X","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-9438-4783","first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","full_name":"Zheden, Vanessa"},{"last_name":"Alanko","full_name":"Alanko, Jonna H","orcid":"0000-0002-7698-3061","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","full_name":"Schur, Florian KM"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"date_published":"2024-03-20T00:00:00Z","day":"20","year":"2024","acknowledgement":"Open Access funding provided by IST Austria. We thank Armel Nicolas and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante, and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the Miba Machine shop for their support. We also thank Wanda Kukulski (University of Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group for helpful discussions. Matt Swulius and Jessica Heebner provided great support in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure illustration.\r\n\r\nThis research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Lab Support Facility, and the Electron Microscopy Facility. We acknowledge funding support from the following sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds (to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt), and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to F.K.M. Schur).","status":"public","ec_funded":1,"file":[{"access_level":"open_access","date_updated":"2024-03-25T12:52:04Z","date_created":"2024-03-25T12:52:04Z","file_size":11907016,"checksum":"90d1984a93660735e506c2a304bc3f73","file_name":"2024_JCB_Zens.pdf","content_type":"application/pdf","creator":"dernst","success":1,"file_id":"15188","relation":"main_file"}],"volume":223,"file_date_updated":"2024-03-25T12:52:04Z","article_number":"e202309125","issue":"6","has_accepted_license":"1","publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"quality_controlled":"1","project":[{"name":"Structure and isoform diversity of the Arp2/3 complex","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","grant_number":"P33367"},{"_id":"7bd318a1-9f16-11ee-852c-cc9217763180","name":"In Situ Actin Structures via Hybrid Cryo-electron Microscopy","grant_number":"E435"},{"call_identifier":"H2020","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"},{"grant_number":"21317","name":"Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis","_id":"2615199A-B435-11E9-9278-68D0E5697425"},{"name":"CryoMinflux-guided in-situ visual proteomics and structure determination","_id":"62909c6f-2b32-11ec-9570-e1476aab5308","grant_number":"CZI01"}],"type":"journal_article","pmid":1,"citation":{"mla":"Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>, vol. 223, no. 6, e202309125, Rockefeller University Press, 2024, doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>.","ama":"Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. 2024;223(6). doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>","ista":"Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V, Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6), e202309125.","apa":"Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V., … Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>","ieee":"B. Zens <i>et al.</i>, “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix,” <i>Journal of Cell Biology</i>, vol. 223, no. 6. Rockefeller University Press, 2024.","short":"B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V. Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).","chicago":"Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2024. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>."},"article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"scopus_import":"1","publication_status":"published","date_updated":"2025-09-04T13:17:16Z","language":[{"iso":"eng"}],"oa_version":"Published Version","doi":"10.1083/jcb.202309125","abstract":[{"text":"The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly.","lang":"eng"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"M-Shop"}],"title":"Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix","external_id":{"pmid":["38506714"],"isi":["001264190100001"]},"month":"03","isi":1,"ddc":["570"],"corr_author":"1","date_created":"2024-03-21T06:45:51Z","publication":"Journal of Cell Biology","oa":1,"intvolume":"       223"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"day":"01","year":"2024","date_published":"2024-09-01T00:00:00Z","publisher":"Elsevier","article_processing_charge":"Yes (in subscription journal)","_id":"15408","author":[{"last_name":"Link","full_name":"Link, Kristina","first_name":"Kristina"},{"last_name":"Muhandes","full_name":"Muhandes, Lina","first_name":"Lina"},{"last_name":"Polikarpova","full_name":"Polikarpova, Anastasia","first_name":"Anastasia"},{"first_name":"Tim","last_name":"Lämmermann","full_name":"Lämmermann, Tim"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"first_name":"Reinhard","full_name":"Fässler, Reinhard","last_name":"Fässler"},{"full_name":"Roers, Axel","last_name":"Roers","first_name":"Axel"}],"file":[{"checksum":"6a5af05082e1869d7cad6406fa4eb76c","content_type":"application/pdf","file_name":"2024_JourAllergyClinicalImm_Link.pdf","creator":"dernst","success":1,"file_id":"18840","relation":"main_file","access_level":"open_access","date_updated":"2025-01-13T10:55:28Z","date_created":"2025-01-13T10:55:28Z","file_size":1792425}],"status":"public","file_date_updated":"2025-01-13T10:55:28Z","volume":154,"acknowledgement":"This work was funded by Deutsche Forschungsgemeinschaft, Germany, grants RO2133/ 9-1 and RO2133/ 9-2 in the setting of FOR2599 and TR156 project C11 (Project-ID 246807620–TRR 156) to A. Roers and Springboard-to-Postdoc grant of the Dresden International Graduate School for Biomedicine and Bioengineering (DIGS-BB), Dresden, Germany, and Fond zur Förderung der Wissenschaftlichen Forschung (FWF), Austria, Hertha Firnberg grant (project number T-1219) to A. Polikarpova.\r\nWe thank Dr Michael Gerlach, Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, TU Dresden, for expert support of in vivo imaging experiments; Grace Wurigamule for help with 2-photon imaging and flow cytometric analysis of mouse skin; and Christina Hiller, Livia Schulze, Madelaine Rickauer, and Christa Haase for providing expert technical assistance.","publication_identifier":{"issn":["0091-6749"],"eissn":["1097-6825"]},"has_accepted_license":"1","issue":"3","citation":{"mla":"Link, Kristina, et al. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>, vol. 154, no. 3, Elsevier, 2024, pp. 745–53, doi:<a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">10.1016/j.jaci.2024.03.022</a>.","ama":"Link K, Muhandes L, Polikarpova A, et al. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. 2024;154(3):745-753. doi:<a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">10.1016/j.jaci.2024.03.022</a>","ista":"Link K, Muhandes L, Polikarpova A, Lämmermann T, Sixt MK, Fässler R, Roers A. 2024. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. Journal of Allergy and Clinical Immunology. 154(3), 745–753.","apa":"Link, K., Muhandes, L., Polikarpova, A., Lämmermann, T., Sixt, M. K., Fässler, R., &#38; Roers, A. (2024). Integrin β1–mediated mast cell immune-surveillance of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">https://doi.org/10.1016/j.jaci.2024.03.022</a>","ieee":"K. Link <i>et al.</i>, “Integrin β1–mediated mast cell immune-surveillance of blood vessel content,” <i>Journal of Allergy and Clinical Immunology</i>, vol. 154, no. 3. Elsevier, pp. 745–753, 2024.","chicago":"Link, Kristina, Lina Muhandes, Anastasia Polikarpova, Tim Lämmermann, Michael K Sixt, Reinhard Fässler, and Axel Roers. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">https://doi.org/10.1016/j.jaci.2024.03.022</a>.","short":"K. Link, L. Muhandes, A. Polikarpova, T. Lämmermann, M.K. Sixt, R. Fässler, A. Roers, Journal of Allergy and Clinical Immunology 154 (2024) 745–753."},"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"OA_type":"hybrid","article_type":"original","type":"journal_article","quality_controlled":"1","pmid":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"scopus_import":"1","date_updated":"2025-09-08T07:28:25Z","publication_status":"published","abstract":[{"lang":"eng","text":"Background: IgE-mediated degranulation of mast cells (MCs) provides rapid protection against environmental hazards, including animal venoms. A fraction of tissue-resident MCs intimately associates with blood vessels. These perivascular MCs were reported to extend projections into the vessel lumen and to be the first MCs to acquire intravenously injected IgE, suggesting that IgE loading of MCs depends on their vascular association.\r\nObjective: We sought to elucidate the molecular basis of the MC–blood vessel interaction and to determine its relevance for IgE-mediated immune responses.\r\nMethods: We selectively inactivated the Itgb1 gene, encoding the β1 chain of integrin adhesion molecules (ITGB1), in MCs by conditional gene targeting in mice. We analyzed skin MCs for blood vessel association, surface IgE density, and capability to bind circulating antibody specific for MC surface molecules, as well as in vivo responses to antigen administered via different routes.\r\nResults: Lack of ITGB1 expression severely compromised MC–blood vessel association. ITGB1-deficient MCs showed normal densities of surface IgE but reduced binding of intravenously injected antibodies. While their capacity to degranulate in response to IgE ligation in vivo was unimpaired, anaphylactic responses to antigen circulating in the vasculature were largely abolished.\r\nConclusions: ITGB1-mediated association of MCs with blood vessels is key for MC immune surveillance of blood vessel content, but is dispensable for slow steady-state loading of endogenous IgE onto tissue-resident MCs."}],"title":"Integrin β1–mediated mast cell immune-surveillance of blood vessel content","external_id":{"isi":["001308886700001"],"pmid":["38636606"]},"doi":"10.1016/j.jaci.2024.03.022","OA_place":"publisher","publication":"Journal of Allergy and Clinical Immunology","page":"745-753","date_created":"2024-05-19T22:01:13Z","ddc":["570"],"isi":1,"month":"09","intvolume":"       154","oa":1},{"publication_status":"published","date_updated":"2025-09-08T08:06:56Z","scopus_import":"1","oa_version":"None","language":[{"iso":"eng"}],"doi":"10.1038/s41590-024-01881-2","external_id":{"isi":["001251509300001"],"pmid":["38907047"]},"title":"Nuclear squeezing wakes up dendritic cells","abstract":[{"lang":"eng","text":"Dendritic cells migrate to and from lymph nodes in response to chemokine gradients.Data now show that steady-state migration of these cells can be triggered by a mechanosensitive pathway."}],"corr_author":"1","isi":1,"month":"06","page":"1131–1132 ","publication":"Nature Immunology","date_created":"2024-06-30T22:01:05Z","intvolume":"        25","article_processing_charge":"No","_id":"17191","author":[{"id":"d993a7b2-292f-11ed-aaac-fb045a912e31","first_name":"Sergio","orcid":"0000-0002-2253-8771","full_name":"Lembo, Sergio","last_name":"Lembo"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"publisher":"Springer Nature","day":"21","year":"2024","date_published":"2024-06-21T00:00:00Z","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":25,"status":"public","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"pmid":1,"type":"journal_article","quality_controlled":"1","article_type":"letter_note","citation":{"mla":"Lembo, Sergio, and Michael K. Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” <i>Nature Immunology</i>, vol. 25, Springer Nature, 2024, pp. 1131–1132, doi:<a href=\"https://doi.org/10.1038/s41590-024-01881-2\">10.1038/s41590-024-01881-2</a>.","ista":"Lembo S, Sixt MK. 2024. Nuclear squeezing wakes up dendritic cells. Nature Immunology. 25, 1131–1132.","ama":"Lembo S, Sixt MK. Nuclear squeezing wakes up dendritic cells. <i>Nature Immunology</i>. 2024;25:1131–1132. doi:<a href=\"https://doi.org/10.1038/s41590-024-01881-2\">10.1038/s41590-024-01881-2</a>","ieee":"S. Lembo and M. K. Sixt, “Nuclear squeezing wakes up dendritic cells,” <i>Nature Immunology</i>, vol. 25. Springer Nature, pp. 1131–1132, 2024.","apa":"Lembo, S., &#38; Sixt, M. K. (2024). Nuclear squeezing wakes up dendritic cells. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-024-01881-2\">https://doi.org/10.1038/s41590-024-01881-2</a>","short":"S. Lembo, M.K. Sixt, Nature Immunology 25 (2024) 1131–1132.","chicago":"Lembo, Sergio, and Michael K Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” <i>Nature Immunology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41590-024-01881-2\">https://doi.org/10.1038/s41590-024-01881-2</a>."}},{"status":"public","file":[{"file_size":2896048,"date_created":"2024-07-16T06:16:11Z","date_updated":"2024-07-16T06:16:11Z","access_level":"open_access","relation":"main_file","file_id":"17242","success":1,"creator":"dernst","content_type":"application/pdf","file_name":"2024_BioProtocol_Li.pdf","checksum":"c8671c0ad483da6407cb16cc3fef1990"}],"volume":14,"file_date_updated":"2024-07-16T06:16:11Z","acknowledgement":"This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (project 772103-BRIDGING to E.M.B.).","department":[{"_id":"MiSi"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2024-07-05T00:00:00Z","year":"2024","day":"05","publisher":"Bio-Protocol","author":[{"full_name":"Li, Ziqiang","last_name":"Li","id":"922e68bb-1727-11ee-857c-966e8cc1b6c3","first_name":"Ziqiang"},{"first_name":"Jennifer","full_name":"Huard, Jennifer","last_name":"Huard"},{"first_name":"Emmanuelle M.","full_name":"Bayer, Emmanuelle M.","last_name":"Bayer"},{"last_name":"Wattelet-Boyer","full_name":"Wattelet-Boyer, Valérie","first_name":"Valérie"}],"_id":"17233","article_processing_charge":"Yes","citation":{"ama":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. Versatile cloning strategy for efficient multigene editing in Arabidopsis. <i>Bio-protocol</i>. 2024;14(13). doi:<a href=\"https://doi.org/10.21769/BioProtoc.5029\">10.21769/BioProtoc.5029</a>","ista":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. 2024. Versatile cloning strategy for efficient multigene editing in Arabidopsis. Bio-protocol. 14(13), e5029.","apa":"LI, Z., Huard, J., Bayer, E. M., &#38; Wattelet-Boyer, V. (2024). Versatile cloning strategy for efficient multigene editing in Arabidopsis. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/BioProtoc.5029\">https://doi.org/10.21769/BioProtoc.5029</a>","ieee":"Z. LI, J. Huard, E. M. Bayer, and V. Wattelet-Boyer, “Versatile cloning strategy for efficient multigene editing in Arabidopsis,” <i>Bio-protocol</i>, vol. 14, no. 13. Bio-Protocol, 2024.","short":"Z. LI, J. Huard, E.M. Bayer, V. Wattelet-Boyer, Bio-Protocol 14 (2024).","chicago":"LI, ZIQIANG, Jennifer Huard, Emmanuelle M. Bayer, and Valérie Wattelet-Boyer. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” <i>Bio-Protocol</i>. Bio-Protocol, 2024. <a href=\"https://doi.org/10.21769/BioProtoc.5029\">https://doi.org/10.21769/BioProtoc.5029</a>.","mla":"LI, ZIQIANG, et al. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” <i>Bio-Protocol</i>, vol. 14, no. 13, e5029, Bio-Protocol, 2024, doi:<a href=\"https://doi.org/10.21769/BioProtoc.5029\">10.21769/BioProtoc.5029</a>."},"article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"quality_controlled":"1","type":"journal_article","pmid":1,"has_accepted_license":"1","publication_identifier":{"eissn":["2331-8325"]},"article_number":"e5029","issue":"13","title":"Versatile cloning strategy for efficient multigene editing in Arabidopsis","abstract":[{"text":"CRISPR-Cas9 technology has become an essential tool for plant genome editing. Recent advancements have significantly improved the ability to target multiple genes simultaneously within the same genetic background through various strategies. Additionally, there has been significant progress in developing methods for inducible or tissue-specific editing. These advancements offer numerous possibilities for tailored genome modifications. Building upon existing research, we have developed an optimized and modular strategy allowing the targeting of several genes simultaneously in combination with the synchronized expression of the Cas9 endonuclease in the egg cell. This system allows significant editing efficiency while avoiding mosaicism. In addition, the versatile system we propose allows adaptation to inducible and/or tissue-specific edition according to the promoter chosen to drive the expression of the Cas9 gene. Here, we describe a step-by-step protocol for generating the binary vector necessary for establishing Arabidopsis edited lines using a versatile cloning strategy that combines Gateway® and Golden Gate technologies. We describe a versatile system that allows the cloning of as many guides as needed to target DNA, which can be multiplexed into a polycistronic gene and combined in the same construct with sequences for the expression of the Cas9 endonuclease. The expression of Cas9 is controlled by selecting from among a collection of promoters, including constitutive, inducible, ubiquitous, or tissue-specific promoters. Only one vector containing the polycistronic gene (tRNA-sgRNA) needs to be constructed. For that, sgRNA (composed of protospacers chosen to target the gene of interest and sgRNA scaffold) is cloned in tandem with the pre-tRNA sequence. Then, a single recombination reaction is required to assemble the promoter, the zCas9 coding sequence, and the tRNA-gRNA polycistronic gene. Each element is cloned in an entry vector and finally assembled according to the Multisite Gateway® Technology. Here, we detail the process to express zCas9 under the control of egg cell promoter fused to enhancer sequence (EC1.2en-EC1.1p) and to simultaneously target two multiple C2 domains and transmembrane region protein genes (MCTP3 and MCTP4, respectively at3g57880 and at1g51570), using one or two sgRNA per gene.","lang":"eng"}],"external_id":{"pmid":["39007160"]},"doi":"10.21769/BioProtoc.5029","language":[{"iso":"eng"}],"oa_version":"Published Version","scopus_import":"1","publication_status":"published","date_updated":"2025-03-06T10:28:18Z","intvolume":"        14","oa":1,"date_created":"2024-07-14T22:01:11Z","publication":"Bio-protocol","month":"07","ddc":["570"]},{"issue":"7","publication_identifier":{"eissn":["2451-9448"],"issn":["2451-9456"]},"type":"journal_article","quality_controlled":"1","pmid":1,"citation":{"mla":"Avellaneda Sarrió, Mario, and Michael K. Sixt. “Rescuing T Cells from Stiff Tumors.” <i>Cell Chemical Biology</i>, vol. 31, no. 7, Elsevier, 2024, pp. 1242–43, doi:<a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">10.1016/j.chembiol.2024.06.011</a>.","short":"M. Avellaneda Sarrió, M.K. Sixt, Cell Chemical Biology 31 (2024) 1242–1243.","chicago":"Avellaneda Sarrió, Mario, and Michael K Sixt. “Rescuing T Cells from Stiff Tumors.” <i>Cell Chemical Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">https://doi.org/10.1016/j.chembiol.2024.06.011</a>.","apa":"Avellaneda Sarrió, M., &#38; Sixt, M. K. (2024). Rescuing T cells from stiff tumors. <i>Cell Chemical Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">https://doi.org/10.1016/j.chembiol.2024.06.011</a>","ieee":"M. Avellaneda Sarrió and M. K. Sixt, “Rescuing T cells from stiff tumors,” <i>Cell Chemical Biology</i>, vol. 31, no. 7. Elsevier, pp. 1242–1243, 2024.","ista":"Avellaneda Sarrió M, Sixt MK. 2024. Rescuing T cells from stiff tumors. Cell Chemical Biology. 31(7), 1242–1243.","ama":"Avellaneda Sarrió M, Sixt MK. Rescuing T cells from stiff tumors. <i>Cell Chemical Biology</i>. 2024;31(7):1242-1243. doi:<a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">10.1016/j.chembiol.2024.06.011</a>"},"article_type":"review","publisher":"Elsevier","_id":"17279","author":[{"last_name":"Avellaneda Sarrió","full_name":"Avellaneda Sarrió, Mario","orcid":"0000-0001-6406-524X","first_name":"Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K"}],"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"year":"2024","day":"18","date_published":"2024-07-18T00:00:00Z","status":"public","volume":31,"isi":1,"month":"07","corr_author":"1","page":"1242-1243","publication":"Cell Chemical Biology","date_created":"2024-07-21T22:01:00Z","intvolume":"        31","scopus_import":"1","date_updated":"2025-09-08T08:27:03Z","publication_status":"published","oa_version":"None","language":[{"iso":"eng"}],"doi":"10.1016/j.chembiol.2024.06.011","title":"Rescuing T cells from stiff tumors","abstract":[{"text":"In a recent issue of Cell, Zhang et al.1 demonstrate that mechanical features of a solid tumor can drive T cells into dysfunctionality and identify pathways that revert this “exhausted” state.","lang":"eng"}],"external_id":{"pmid":["39029454"],"isi":["001275725000001"]}},{"doi":"10.1038/s41586-024-07671-y","title":"Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis","abstract":[{"lang":"eng","text":"Platelet homeostasis is essential for vascular integrity and immune defence1,2. Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, the cellular and molecular mechanisms required to constantly replenish the pool of MKs by their progenitor cells (megakaryopoiesis) remains unclear3,4. Here we use intravital imaging to track the cellular dynamics of megakaryopoiesis over days. We identify plasmacytoid dendritic cells (pDCs) as homeostatic sensors that monitor the bone marrow for apoptotic MKs and deliver IFNα to the MK niche triggering local on-demand proliferation and maturation of MK progenitors. This pDC-dependent feedback loop is crucial for MK and platelet homeostasis at steady state and under stress. pDCs are best known for their ability to function as vigilant detectors of viral infection5. We show that virus-induced activation of pDCs interferes with their function as homeostatic sensors of megakaryopoiesis. Consequently, activation of pDCs by SARS-CoV-2 leads to excessive megakaryopoiesis. Together, we identify a pDC-dependent homeostatic circuit that involves innate immune sensing and demand-adapted release of inflammatory mediators to maintain homeostasis of the megakaryocytic lineage."}],"external_id":{"isi":["001281636500020"],"pmid":["38987596"]},"scopus_import":"1","date_updated":"2025-09-08T08:14:25Z","publication_status":"published","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"intvolume":"       631","month":"07","ddc":["570"],"isi":1,"corr_author":"1","date_created":"2024-07-21T22:01:02Z","publication":"Nature","page":"645-653","acknowledgement":"We thank S. Helmer, N. Blount, E. Raatz and Z. Sisic for technical assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) SFB 1123 (S.M. project B06); SFB 914 (S.M. projects B02 and Z01, H.I.-A. project Z01, S.S. project A06, K.S. project B02, C. Schulz project A10, B.W. project A02, C. Scheiermann project B09); SFB 1054 (T.B. project B03); FOR2033 (F.G., R.A.J.O., S.M.); Individual research grant project ID: 514478744 (F.G.); Heisenberg Programme project ID: 514477451 (F.G.); the DZHK (German Center for Cardiovascular Research) (MHA 1.4VD (S.M.), Postdoc Start-up Grant, 81×3600213 (F.G.)); and LMUexcellence NFF (F.G.). W.F. received funding from China Scholarship Council (CSC, no. 201306270012). P.B. is supported by the German Research Foundation (DFG, project IDs 322900939, 432698239 and 445703531), European Research Council (ERC Consolidator grant no. 101001791) and the Federal Ministry of Education and Research (BMBF, STOP-FSGS-01GM2202C and NATON within the framework of the Network of University Medicine, no. 01KX2121). S.v.S. is supported by the START-Program of the Faculty of Medicine of the RWTH Aachen University (AZ 125/17). A.D. and S.E. are supported by the German Research Foundation (SFB TRR 267); S.E. by the BMBF in the framework of the Cluster4future program (CNATM—Cluster for Nucleic Acid Therapeutics Munich). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 833440 to S.M.). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687. The project is funded by the European Union (ERC, MEKanics, 101078110). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.","ec_funded":1,"status":"public","file":[{"file_size":15704819,"date_created":"2024-07-22T06:16:11Z","access_level":"open_access","date_updated":"2024-07-22T06:16:11Z","file_id":"17286","relation":"main_file","content_type":"application/pdf","file_name":"2024_Nature_Gaertner.pdf","checksum":"aa004afc72d2489f0fb0fcbc9919fbbd","creator":"dernst","success":1}],"volume":631,"file_date_updated":"2024-07-22T06:16:11Z","publisher":"Springer Nature","author":[{"last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R"},{"first_name":"Hellen","full_name":"Ishikawa-Ankerhold, Hellen","last_name":"Ishikawa-Ankerhold"},{"first_name":"Susanne","last_name":"Stutte","full_name":"Stutte, Susanne"},{"last_name":"Fu","full_name":"Fu, Wenwen","first_name":"Wenwen"},{"first_name":"Jutta","last_name":"Weitz","full_name":"Weitz, Jutta"},{"first_name":"Anne","last_name":"Dueck","full_name":"Dueck, Anne"},{"first_name":"Bhavishya","full_name":"Nelakuditi, Bhavishya","last_name":"Nelakuditi"},{"first_name":"Valeria","last_name":"Fumagalli","full_name":"Fumagalli, Valeria"},{"first_name":"Dominic","full_name":"Van Den Heuvel, Dominic","last_name":"Van Den Heuvel"},{"full_name":"Belz, Larissa","last_name":"Belz","first_name":"Larissa"},{"first_name":"Gulnoza","last_name":"Sobirova","full_name":"Sobirova, Gulnoza"},{"last_name":"Zhang","full_name":"Zhang, Zhe","first_name":"Zhe"},{"full_name":"Titova, Anna","last_name":"Titova","first_name":"Anna"},{"last_name":"Navarro","full_name":"Navarro, Alejandro Martinez","first_name":"Alejandro Martinez"},{"first_name":"Kami","full_name":"Pekayvaz, Kami","last_name":"Pekayvaz"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"last_name":"Von Baumgarten","full_name":"Von Baumgarten, Louisa","first_name":"Louisa"},{"full_name":"Kranich, Jan","last_name":"Kranich","first_name":"Jan"},{"first_name":"Tobias","full_name":"Straub, Tobias","last_name":"Straub"},{"full_name":"Popper, Bastian","last_name":"Popper","first_name":"Bastian"},{"orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Zheden","full_name":"Zheden, Vanessa"},{"orcid":"0000-0001-9735-5315","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","full_name":"Kaufmann, Walter"},{"last_name":"Guo","full_name":"Guo, Chenglong","first_name":"Chenglong"},{"last_name":"Piontek","full_name":"Piontek, Guido","first_name":"Guido"},{"last_name":"Von Stillfried","full_name":"Von Stillfried, Saskia","first_name":"Saskia"},{"first_name":"Peter","full_name":"Boor, Peter","last_name":"Boor"},{"last_name":"Colonna","full_name":"Colonna, Marco","first_name":"Marco"},{"last_name":"Clauß","full_name":"Clauß, Sebastian","first_name":"Sebastian"},{"last_name":"Schulz","full_name":"Schulz, Christian","first_name":"Christian"},{"first_name":"Thomas","full_name":"Brocker, Thomas","last_name":"Brocker"},{"first_name":"Barbara","last_name":"Walzog","full_name":"Walzog, Barbara"},{"first_name":"Christoph","full_name":"Scheiermann, Christoph","last_name":"Scheiermann"},{"full_name":"Aird, William C.","last_name":"Aird","first_name":"William C."},{"last_name":"Nerlov","full_name":"Nerlov, Claus","first_name":"Claus"},{"first_name":"Konstantin","full_name":"Stark, Konstantin","last_name":"Stark"},{"last_name":"Petzold","full_name":"Petzold, Tobias","first_name":"Tobias"},{"last_name":"Engelhardt","full_name":"Engelhardt, Stefan","first_name":"Stefan"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"full_name":"Rudelius, Martina","last_name":"Rudelius","first_name":"Martina"},{"first_name":"Robert A.J.","last_name":"Oostendorp","full_name":"Oostendorp, Robert A.J."},{"full_name":"Iannacone, Matteo","last_name":"Iannacone","first_name":"Matteo"},{"first_name":"Matthias","last_name":"Heinig","full_name":"Heinig, Matthias"},{"first_name":"Steffen","last_name":"Massberg","full_name":"Massberg, Steffen"}],"_id":"17284","article_processing_charge":"Yes (in subscription journal)","department":[{"_id":"EM-Fac"},{"_id":"MiSi"},{"_id":"Bio"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-07-18T00:00:00Z","day":"18","year":"2024","quality_controlled":"1","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","grant_number":"747687"}],"type":"journal_article","pmid":1,"citation":{"mla":"Gärtner, Florian R., et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>, vol. 631, Springer Nature, 2024, pp. 645–53, doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>.","ama":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. 2024;631:645-653. doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>","ista":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, Fu W, Weitz J, Dueck A, Nelakuditi B, Fumagalli V, Van Den Heuvel D, Belz L, Sobirova G, Zhang Z, Titova A, Navarro AM, Pekayvaz K, Lorenz M, Von Baumgarten L, Kranich J, Straub T, Popper B, Zheden V, Kaufmann W, Guo C, Piontek G, Von Stillfried S, Boor P, Colonna M, Clauß S, Schulz C, Brocker T, Walzog B, Scheiermann C, Aird WC, Nerlov C, Stark K, Petzold T, Engelhardt S, Sixt MK, Hauschild R, Rudelius M, Oostendorp RAJ, Iannacone M, Heinig M, Massberg S. 2024. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. 631, 645–653.","short":"F.R. Gärtner, H. Ishikawa-Ankerhold, S. Stutte, W. Fu, J. Weitz, A. Dueck, B. Nelakuditi, V. Fumagalli, D. Van Den Heuvel, L. Belz, G. Sobirova, Z. Zhang, A. Titova, A.M. Navarro, K. Pekayvaz, M. Lorenz, L. Von Baumgarten, J. Kranich, T. Straub, B. Popper, V. Zheden, W. Kaufmann, C. Guo, G. Piontek, S. Von Stillfried, P. Boor, M. Colonna, S. Clauß, C. Schulz, T. Brocker, B. Walzog, C. Scheiermann, W.C. Aird, C. Nerlov, K. Stark, T. Petzold, S. Engelhardt, M.K. Sixt, R. Hauschild, M. Rudelius, R.A.J. Oostendorp, M. Iannacone, M. Heinig, S. Massberg, Nature 631 (2024) 645–653.","chicago":"Gärtner, Florian R, Hellen Ishikawa-Ankerhold, Susanne Stutte, Wenwen Fu, Jutta Weitz, Anne Dueck, Bhavishya Nelakuditi, et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>.","apa":"Gärtner, F. R., Ishikawa-Ankerhold, H., Stutte, S., Fu, W., Weitz, J., Dueck, A., … Massberg, S. (2024). Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>","ieee":"F. R. Gärtner <i>et al.</i>, “Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis,” <i>Nature</i>, vol. 631. Springer Nature, pp. 645–653, 2024."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","related_material":{"link":[{"url":"https://github.com/heiniglab/gaertner_megakaryocytes","relation":"software"}]},"has_accepted_license":"1","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]}}]