[{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"date_published":"2014-05-14T00:00:00Z","year":"2014","day":"14","publisher":"Nature Publishing Group","author":[{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","first_name":"Jörg","orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg","last_name":"Renkawitz"},{"first_name":"Claudio","last_name":"Lademann","full_name":"Lademann, Claudio"},{"first_name":"Stefan","last_name":"Jentsch","full_name":"Jentsch, Stefan"}],"_id":"2215","article_processing_charge":"No","status":"public","volume":15,"acknowledgement":"J.R. was supported by a Boehringer Ingelheim Fonds PhD stipend.","publist_id":"4755","issue":"6","citation":{"mla":"Renkawitz, Jörg, et al. “Mechanisms and Principles of Homology Search during Recombination.” <i>Nature Reviews Molecular Cell Biology</i>, vol. 15, no. 6, Nature Publishing Group, 2014, pp. 369–83, doi:<a href=\"https://doi.org/10.1038/nrm3805\">10.1038/nrm3805</a>.","ista":"Renkawitz J, Lademann C, Jentsch S. 2014. Mechanisms and principles of homology search during recombination. Nature Reviews Molecular Cell Biology. 15(6), 369–383.","ama":"Renkawitz J, Lademann C, Jentsch S. Mechanisms and principles of homology search during recombination. <i>Nature Reviews Molecular Cell Biology</i>. 2014;15(6):369-383. doi:<a href=\"https://doi.org/10.1038/nrm3805\">10.1038/nrm3805</a>","chicago":"Renkawitz, Jörg, Claudio Lademann, and Stefan Jentsch. “Mechanisms and Principles of Homology Search during Recombination.” <i>Nature Reviews Molecular Cell Biology</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nrm3805\">https://doi.org/10.1038/nrm3805</a>.","short":"J. Renkawitz, C. Lademann, S. Jentsch, Nature Reviews Molecular Cell Biology 15 (2014) 369–383.","ieee":"J. Renkawitz, C. Lademann, and S. Jentsch, “Mechanisms and principles of homology search during recombination,” <i>Nature Reviews Molecular Cell Biology</i>, vol. 15, no. 6. Nature Publishing Group, pp. 369–383, 2014.","apa":"Renkawitz, J., Lademann, C., &#38; Jentsch, S. (2014). Mechanisms and principles of homology search during recombination. <i>Nature Reviews Molecular Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nrm3805\">https://doi.org/10.1038/nrm3805</a>"},"quality_controlled":"1","type":"journal_article","language":[{"iso":"eng"}],"oa_version":"None","scopus_import":"1","publication_status":"published","date_updated":"2025-09-29T11:30:10Z","abstract":[{"lang":"eng","text":"Homologous recombination is crucial for genome stability and for genetic exchange. Although our knowledge of the principle steps in recombination and its machinery is well advanced, homology search, the critical step of exploring the genome for homologous sequences to enable recombination, has remained mostly enigmatic. However, recent methodological advances have provided considerable new insights into this fundamental step in recombination that can be integrated into a mechanistic model. These advances emphasize the importance of genomic proximity and nuclear organization for homology search and the critical role of homology search mediators in this process. They also aid our understanding of how homology search might lead to unwanted and potentially disease-promoting recombination events."}],"title":"Mechanisms and principles of homology search during recombination","external_id":{"isi":["000337245500009"]},"doi":"10.1038/nrm3805","date_created":"2018-12-11T11:56:22Z","publication":"Nature Reviews Molecular Cell Biology","page":"369 - 383","month":"05","isi":1,"intvolume":"        15"},{"external_id":{"isi":["000331115700016"]},"title":"A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation","abstract":[{"text":"MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR-155 on the expression levels of other miRNAs. For example, miR-455 is negatively regulated in miR-155-deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR-155 in DCs and macrophages.","lang":"eng"}],"doi":"10.1016/j.febslet.2014.01.009","language":[{"iso":"eng"}],"oa_version":"None","publication_status":"published","date_updated":"2025-09-29T11:19:23Z","scopus_import":"1","intvolume":"       588","date_created":"2018-12-11T11:56:31Z","publication":"FEBS Letters","page":"632 - 640","month":"02","isi":1,"volume":588,"status":"public","publist_id":"4714","date_published":"2014-02-14T00:00:00Z","day":"14","year":"2014","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"2242","article_processing_charge":"No","author":[{"first_name":"Anne","last_name":"Dueck","full_name":"Dueck, Anne"},{"first_name":"Alexander","id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","full_name":"Eichner, Alexander","last_name":"Eichner"},{"full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"last_name":"Meister","full_name":"Meister, Gunter","first_name":"Gunter"}],"publisher":"Elsevier","citation":{"mla":"Dueck, Anne, et al. “A MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell Maturation and Macrophage Activation.” <i>FEBS Letters</i>, vol. 588, no. 4, Elsevier, 2014, pp. 632–40, doi:<a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">10.1016/j.febslet.2014.01.009</a>.","ieee":"A. Dueck, A. Eichner, M. K. Sixt, and G. Meister, “A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation,” <i>FEBS Letters</i>, vol. 588, no. 4. Elsevier, pp. 632–640, 2014.","apa":"Dueck, A., Eichner, A., Sixt, M. K., &#38; Meister, G. (2014). A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">https://doi.org/10.1016/j.febslet.2014.01.009</a>","short":"A. Dueck, A. Eichner, M.K. Sixt, G. Meister, FEBS Letters 588 (2014) 632–640.","chicago":"Dueck, Anne, Alexander Eichner, Michael K Sixt, and Gunter Meister. “A MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell Maturation and Macrophage Activation.” <i>FEBS Letters</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">https://doi.org/10.1016/j.febslet.2014.01.009</a>.","ama":"Dueck A, Eichner A, Sixt MK, Meister G. A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. <i>FEBS Letters</i>. 2014;588(4):632-640. doi:<a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">10.1016/j.febslet.2014.01.009</a>","ista":"Dueck A, Eichner A, Sixt MK, Meister G. 2014. A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. FEBS Letters. 588(4), 632–640."},"quality_controlled":"1","type":"journal_article","publication_identifier":{"issn":["0014-5793"]},"issue":"4"},{"month":"05","isi":1,"corr_author":"1","date_created":"2018-12-11T11:59:49Z","page":"853 - 854","publication":"Immunity","intvolume":"        38","scopus_import":"1","date_updated":"2025-09-29T13:51:00Z","publication_status":"published","language":[{"iso":"eng"}],"oa_version":"None","doi":"10.1016/j.immuni.2013.05.005","title":"A conduit to amplify innate immunity","external_id":{"isi":["000330942500005"]},"issue":"5","quality_controlled":"1","type":"journal_article","citation":{"mla":"Moussion, Christine, and Michael K. Sixt. “A Conduit to Amplify Innate Immunity.” <i>Immunity</i>, vol. 38, no. 5, Cell Press, 2013, pp. 853–54, doi:<a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">10.1016/j.immuni.2013.05.005</a>.","ama":"Moussion C, Sixt MK. A conduit to amplify innate immunity. <i>Immunity</i>. 2013;38(5):853-854. doi:<a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">10.1016/j.immuni.2013.05.005</a>","ista":"Moussion C, Sixt MK. 2013. A conduit to amplify innate immunity. Immunity. 38(5), 853–854.","chicago":"Moussion, Christine, and Michael K Sixt. “A Conduit to Amplify Innate Immunity.” <i>Immunity</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">https://doi.org/10.1016/j.immuni.2013.05.005</a>.","short":"C. Moussion, M.K. Sixt, Immunity 38 (2013) 853–854.","apa":"Moussion, C., &#38; Sixt, M. K. (2013). A conduit to amplify innate immunity. <i>Immunity</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">https://doi.org/10.1016/j.immuni.2013.05.005</a>","ieee":"C. Moussion and M. K. Sixt, “A conduit to amplify innate immunity,” <i>Immunity</i>, vol. 38, no. 5. Cell Press, pp. 853–854, 2013."},"publisher":"Cell Press","_id":"2830","article_processing_charge":"No","author":[{"full_name":"Moussion, Christine","last_name":"Moussion","first_name":"Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2013-05-23T00:00:00Z","day":"23","year":"2013","publist_id":"3969","status":"public","volume":38},{"year":"2013","day":"18","date_published":"2013-01-18T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"},{"_id":"Bio"}],"author":[{"first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","full_name":"Weber, Michele","last_name":"Weber"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Jan","last_name":"Schwarz"},{"first_name":"Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87","last_name":"Moussion","full_name":"Moussion, Christine"},{"first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","last_name":"De Vries"},{"first_name":"Daniel","last_name":"Legler","full_name":"Legler, Daniel"},{"last_name":"Luther","full_name":"Luther, Sanjiv","first_name":"Sanjiv"},{"orcid":"0000-0003-4398-476X","first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"}],"_id":"2839","article_processing_charge":"No","publisher":"American Association for the Advancement of Science","volume":339,"ec_funded":1,"status":"public","publist_id":"3959","acknowledgement":"We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst, and E. Kiermaier for critical reading of the manuscript. This work was supported by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011 to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).","issue":"6117","main_file_link":[{"open_access":"1","url":"https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf"}],"article_type":"original","citation":{"ieee":"M. Weber <i>et al.</i>, “Interstitial dendritic cell guidance by haptotactic chemokine gradients,” <i>Science</i>, vol. 339, no. 6117. American Association for the Advancement of Science, pp. 328–332, 2013.","apa":"Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler, D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>","chicago":"Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>. American Association for the Advancement of Science, 2013. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>.","short":"M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler, S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.","ama":"Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. 2013;339(6117):328-332. doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>","ista":"Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. 339(6117), 328–332.","mla":"Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>, vol. 339, no. 6117, American Association for the Advancement of Science, 2013, pp. 328–32, doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>."},"type":"journal_article","project":[{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7"},{"_id":"25ABD200-B435-11E9-9278-68D0E5697425","name":"Cell migration in complex environments: from in vivo experiments to theoretical models","grant_number":"RGP0058/2011"}],"quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2025-09-29T13:45:52Z","scopus_import":"1","external_id":{"isi":["000313622000047"]},"abstract":[{"lang":"eng","text":"Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues."}],"title":"Interstitial dendritic cell guidance by haptotactic chemokine gradients","doi":"10.1126/science.1228456","publication":"Science","page":"328 - 332","date_created":"2018-12-11T11:59:52Z","corr_author":"1","isi":1,"month":"01","intvolume":"       339","oa":1},{"doi":"10.1007/978-1-62703-426-5_14","external_id":{"pmid":["23625502"]},"abstract":[{"text":"Leukocyte migration through the interstitial space is crucial for the maintenance of tolerance and immunity. The main cues for leukocyte trafficking are chemokines thought to directionally guide these cells towards their targets. However, model systems that facilitate quantification of chemokine-guided leukocyte migration in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted mouse ears that allows the visualization of chemokine-dependent dendritic cell (DC) motility in the dermal interstitium in real time. We present methods for the preparation of mouse ear sheets and their use in multidimensional confocal imaging experiments to monitor and analyze the directional migration of fluorescently labelled DCs through the dermis and into afferent lymphatic vessels. The assay provides a more physiological approach to study leukocyte migration than in vitro three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen gels and transwell assays.","lang":"eng"}],"title":"Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations","date_updated":"2024-10-09T21:02:37Z","publication_status":"published","series_title":"MIMB","scopus_import":"1","oa_version":"None","language":[{"iso":"eng"}],"intvolume":"      1013","corr_author":"1","editor":[{"first_name":"Astrid","full_name":"Cardona, Astrid","last_name":"Cardona"},{"full_name":"Ubogu, Eroboghene","last_name":"Ubogu","first_name":"Eroboghene"}],"month":"04","page":"215-226","publication":"Chemokines","date_created":"2022-03-21T07:47:41Z","acknowledgement":"We would like to thank Alexander Eichner and Ingrid de Vries for discussion and critical reading of the manuscript, and Mary Frank for assistance with the recording of videos and images in Fig. 1. M.S. is supported through funding from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt Foundation for funding.","volume":1013,"status":"public","author":[{"first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","last_name":"Weber","full_name":"Weber, Michele"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"_id":"10900","article_processing_charge":"No","publisher":"Humana Press","alternative_title":["Methods in Molecular Biology"],"year":"2013","day":"03","date_published":"2013-04-03T00:00:00Z","place":"Totowa, NJ","department":[{"_id":"MiSi"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","pmid":1,"type":"book_chapter","quality_controlled":"1","citation":{"mla":"Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26, doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>.","ama":"Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. <i>Chemokines</i>. Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>","ista":"Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology, vol. 1013, 215–226.","chicago":"Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ: Humana Press, 2013. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>.","short":"M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana Press, Totowa, NJ, 2013, pp. 215–226.","ieee":"M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations,” in <i>Chemokines</i>, vol. 1013, A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.","apa":"Weber, M., &#38; Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona &#38; E. Ubogu (Eds.), <i>Chemokines</i> (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>"},"publication_identifier":{"issn":["1064-3745"],"eisbn":["9781627034265"],"isbn":["9781627034258"],"eissn":["1940-6029"]}},{"oa_version":"None","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2025-09-30T07:11:09Z","scopus_import":"1","external_id":{"isi":["000326559400006"]},"abstract":[{"lang":"eng","text":"Podoplanin, a mucin-like plasma membrane protein, is expressed by lymphatic endothelial cells and responsible for separation of blood and lymphatic circulation through activation of platelets. Here we show that podoplanin is also expressed by thymic fibroblastic reticular cells (tFRC), a novel thymic medulla stroma cell type associated with thymic conduits, and involved in development of natural regulatory T cells (nTreg). Young mice deficient in podoplanin lack nTreg owing to retardation of CD4+CD25+ thymocytes in the cortex and missing differentiation of Foxp3+ thymocytes in the medulla. This might be due to CCL21 that delocalizes upon deletion of the CCL21-binding podoplanin from medullar tFRC to cortex areas. The animals do not remain devoid of nTreg but generate them delayed within the first month resulting in Th2-biased hypergammaglobulinemia but not in the death-causing autoimmune phenotype of Foxp3-deficient Scurfy mice."}],"title":"Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells","doi":"10.1016/j.imlet.2013.07.007","publication":"Immunology Letters","page":"31 - 41","date_created":"2018-12-11T11:46:57Z","isi":1,"month":"07","intvolume":"       154","day":"01","year":"2013","date_published":"2013-07-01T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"_id":"522","author":[{"full_name":"Fuertbauer, Elke","last_name":"Fuertbauer","first_name":"Elke"},{"first_name":"Jan","full_name":"Zaujec, Jan","last_name":"Zaujec"},{"full_name":"Uhrin, Pavel","last_name":"Uhrin","first_name":"Pavel"},{"last_name":"Raab","full_name":"Raab, Ingrid","first_name":"Ingrid"},{"last_name":"Weber","full_name":"Weber, Michele","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Helga","full_name":"Schachner, Helga","last_name":"Schachner"},{"full_name":"Bauer, Miroslav","last_name":"Bauer","first_name":"Miroslav"},{"first_name":"Gerhard","full_name":"Schütz, Gerhard","last_name":"Schütz"},{"last_name":"Binder","full_name":"Binder, Bernd","first_name":"Bernd"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"full_name":"Kerjaschki, Dontscho","last_name":"Kerjaschki","first_name":"Dontscho"},{"first_name":"Hannes","full_name":"Stockinger, Hannes","last_name":"Stockinger"}],"article_processing_charge":"No","publisher":"Elsevier","volume":154,"status":"public","publist_id":"7300","issue":"1-2","citation":{"ieee":"E. Fuertbauer <i>et al.</i>, “Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells,” <i>Immunology Letters</i>, vol. 154, no. 1–2. Elsevier, pp. 31–41, 2013.","apa":"Fuertbauer, E., Zaujec, J., Uhrin, P., Raab, I., Weber, M., Schachner, H., … Stockinger, H. (2013). Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>","chicago":"Fuertbauer, Elke, Jan Zaujec, Pavel Uhrin, Ingrid Raab, Michele Weber, Helga Schachner, Miroslav Bauer, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>.","short":"E. Fuertbauer, J. Zaujec, P. Uhrin, I. Raab, M. Weber, H. Schachner, M. Bauer, G. Schütz, B. Binder, M.K. Sixt, D. Kerjaschki, H. Stockinger, Immunology Letters 154 (2013) 31–41.","ama":"Fuertbauer E, Zaujec J, Uhrin P, et al. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. 2013;154(1-2):31-41. doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>","ista":"Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, Bauer M, Schütz G, Binder B, Sixt MK, Kerjaschki D, Stockinger H. 2013. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. Immunology Letters. 154(1–2), 31–41.","mla":"Fuertbauer, Elke, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>, vol. 154, no. 1–2, Elsevier, 2013, pp. 31–41, doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>."},"type":"journal_article","quality_controlled":"1"},{"volume":12,"status":"public","publist_id":"3787","acknowledgement":"We thank M. Sixt and A. Peixoto for helpful comments on the manuscript. Work in the laboratory of J.-P.G. is supported by grants from Fondation ARC pour la Recherche sur le Cancer, Agence Nationale de la Recherche (ANR), Institut National du Cancer (INCA), Fondation RITC and Région Midi-Pyrénées. Research by R.F. is supported by Deutsche Forschungsgemeinschaft (DFG) grants SFB621-A1, SFB738-B5, SFB587-B3, SFB900-B1 and KFO 250-FO 334/2-1. We regret that, owing to space limitations, we could not always quote the work of colleagues who have contributed to the field.","year":"2012","day":"01","date_published":"2012-11-01T00:00:00Z","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"No","_id":"2945","author":[{"first_name":"Jean","last_name":"Girard","full_name":"Girard, Jean"},{"first_name":"Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87","full_name":"Moussion, Christine","last_name":"Moussion"},{"last_name":"Förster","full_name":"Förster, Reinhold","first_name":"Reinhold"}],"publisher":"Nature Publishing Group","citation":{"mla":"Girard, Jean, et al. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking in Lymph Nodes.” <i>Nature Reviews Immunology</i>, vol. 12, no. 11, Nature Publishing Group, 2012, pp. 762–73, doi:<a href=\"https://doi.org/10.1038/nri3298\">10.1038/nri3298</a>.","ista":"Girard J, Moussion C, Förster R. 2012. HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. Nature Reviews Immunology. 12(11), 762–773.","ama":"Girard J, Moussion C, Förster R. HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. <i>Nature Reviews Immunology</i>. 2012;12(11):762-773. doi:<a href=\"https://doi.org/10.1038/nri3298\">10.1038/nri3298</a>","chicago":"Girard, Jean, Christine Moussion, and Reinhold Förster. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking in Lymph Nodes.” <i>Nature Reviews Immunology</i>. Nature Publishing Group, 2012. <a href=\"https://doi.org/10.1038/nri3298\">https://doi.org/10.1038/nri3298</a>.","short":"J. Girard, C. Moussion, R. Förster, Nature Reviews Immunology 12 (2012) 762–773.","ieee":"J. Girard, C. Moussion, and R. Förster, “HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes,” <i>Nature Reviews Immunology</i>, vol. 12, no. 11. Nature Publishing Group, pp. 762–773, 2012.","apa":"Girard, J., Moussion, C., &#38; Förster, R. (2012). HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. <i>Nature Reviews Immunology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nri3298\">https://doi.org/10.1038/nri3298</a>"},"type":"journal_article","quality_controlled":"1","issue":"11","external_id":{"isi":["000310523400010"]},"title":"HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes","abstract":[{"lang":"eng","text":"In search of foreign antigens, lymphocytes recirculate from the blood, through lymph nodes, into lymphatics and back to the blood. Dendritic cells also migrate to lymph nodes for optimal interaction with lymphocytes. This continuous trafficking of immune cells into and out of lymph nodes is essential for immune surveillance of foreign invaders. In this article, we review our current understanding of the functions of high endothelial venules (HEVs), stroma and lymphatics in the entry, positioning and exit of immune cells in lymph nodes during homeostasis, and we highlight the unexpected role of dendritic cells in the control of lymphocyte homing through HEVs."}],"doi":"10.1038/nri3298","oa_version":"None","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2025-09-30T08:12:46Z","scopus_import":"1","intvolume":"        12","publication":"Nature Reviews Immunology","page":"762 - 773","date_created":"2018-12-11T12:00:29Z","isi":1,"month":"11"},{"issue":"19","has_accepted_license":"1","type":"journal_article","quality_controlled":"1","citation":{"ieee":"A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, and G. Meister, “MicroRNAs associated with the different human Argonaute proteins,” <i>Nucleic Acids Research</i>, vol. 40, no. 19. Oxford University Press, pp. 9850–9862, 2012.","apa":"Dueck, A., Ziegler, C., Eichner, A., Berezikov, E., &#38; Meister, G. (2012). MicroRNAs associated with the different human Argonaute proteins. <i>Nucleic Acids Research</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/nar/gks705\">https://doi.org/10.1093/nar/gks705</a>","short":"A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, G. Meister, Nucleic Acids Research 40 (2012) 9850–9862.","chicago":"Dueck, Anne, Christian Ziegler, Alexander Eichner, Eugène Berezikov, and Gunter Meister. “MicroRNAs Associated with the Different Human Argonaute Proteins.” <i>Nucleic Acids Research</i>. Oxford University Press, 2012. <a href=\"https://doi.org/10.1093/nar/gks705\">https://doi.org/10.1093/nar/gks705</a>.","ista":"Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. 2012. MicroRNAs associated with the different human Argonaute proteins. Nucleic Acids Research. 40(19), 9850–9862.","ama":"Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. MicroRNAs associated with the different human Argonaute proteins. <i>Nucleic Acids Research</i>. 2012;40(19):9850-9862. doi:<a href=\"https://doi.org/10.1093/nar/gks705\">10.1093/nar/gks705</a>","mla":"Dueck, Anne, et al. “MicroRNAs Associated with the Different Human Argonaute Proteins.” <i>Nucleic Acids Research</i>, vol. 40, no. 19, Oxford University Press, 2012, pp. 9850–62, doi:<a href=\"https://doi.org/10.1093/nar/gks705\">10.1093/nar/gks705</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"publisher":"Oxford University Press","author":[{"last_name":"Dueck","full_name":"Dueck, Anne","first_name":"Anne"},{"first_name":"Christian","full_name":"Ziegler, Christian","last_name":"Ziegler"},{"first_name":"Alexander","id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","last_name":"Eichner","full_name":"Eichner, Alexander"},{"first_name":"Eugène","full_name":"Berezikov, Eugène","last_name":"Berezikov"},{"first_name":"Gunter","full_name":"Meister, Gunter","last_name":"Meister"}],"_id":"2946","article_processing_charge":"No","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"01","year":"2012","date_published":"2012-10-01T00:00:00Z","pubrep_id":"383","acknowledgement":"Deutsche Forschungsgemeinschaft (DFG) (SFB 960 and FOR855); European Research Council (ERC grant ‘sRNAs’); European Union (FP7 project ‘ONCOMIRs’); German Bundesministerium für Bildung und Forschung (BMBF, NGFN+, FKZ PIM-01GS0804-5); Bavarian Genome Research Network (BayGene to G.M.); The Netherlands Organization for Scientific Research (NWO, VIDI grant to E.B.). Funding for open access charge: DFG via the open access publishing program. \r\n\r\nWe thank Sigrun Ammon and Corinna Friederich for technical assistance and Sebastian Petri and Daniel Schraivogel for helpful discussions.","publist_id":"3786","file":[{"date_created":"2018-12-12T10:13:12Z","file_size":8126936,"date_updated":"2020-07-14T12:45:55Z","access_level":"open_access","relation":"main_file","file_id":"4993","creator":"system","content_type":"application/pdf","file_name":"IST-2015-383-v1+1_Nucl._Acids_Res.-2012-Dueck-9850-62.pdf","checksum":"1bb8d1ff894014b481657a21083c941c"}],"status":"public","file_date_updated":"2020-07-14T12:45:55Z","volume":40,"isi":1,"ddc":["570"],"month":"10","corr_author":"1","page":"9850 - 9862","publication":"Nucleic Acids Research","date_created":"2018-12-11T12:00:29Z","oa":1,"intvolume":"        40","scopus_import":"1","publication_status":"published","date_updated":"2025-09-30T08:12:07Z","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1093/nar/gks705","title":"MicroRNAs associated with the different human Argonaute proteins","abstract":[{"lang":"eng","text":"MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3′-untranslated region (3′-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected."}],"external_id":{"isi":["000310377200046"]}},{"issue":"11-12","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930012/","open_access":"1"}],"citation":{"chicago":"Schachtner, Hannah, Ang Li, David Stevenson, Simon Calaminus, Steven Thomas, Steve Watson, Michael K Sixt, Roland Wedlich Söldner, Douglas Strathdee, and Laura Machesky. “Tissue Inducible Lifeact Expression Allows Visualization of Actin Dynamics in Vivo and Ex Vivo.” <i>European Journal of Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">https://doi.org/10.1016/j.ejcb.2012.04.002</a>.","short":"H. Schachtner, A. Li, D. Stevenson, S. Calaminus, S. Thomas, S. Watson, M.K. Sixt, R. Wedlich Söldner, D. Strathdee, L. Machesky, European Journal of Cell Biology 91 (2012) 923–929.","apa":"Schachtner, H., Li, A., Stevenson, D., Calaminus, S., Thomas, S., Watson, S., … Machesky, L. (2012). Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. <i>European Journal of Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">https://doi.org/10.1016/j.ejcb.2012.04.002</a>","ieee":"H. Schachtner <i>et al.</i>, “Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo,” <i>European Journal of Cell Biology</i>, vol. 91, no. 11–12. Elsevier, pp. 923–929, 2012.","ama":"Schachtner H, Li A, Stevenson D, et al. Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. <i>European Journal of Cell Biology</i>. 2012;91(11-12):923-929. doi:<a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">10.1016/j.ejcb.2012.04.002</a>","ista":"Schachtner H, Li A, Stevenson D, Calaminus S, Thomas S, Watson S, Sixt MK, Wedlich Söldner R, Strathdee D, Machesky L. 2012. Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European Journal of Cell Biology. 91(11–12), 923–929.","mla":"Schachtner, Hannah, et al. “Tissue Inducible Lifeact Expression Allows Visualization of Actin Dynamics in Vivo and Ex Vivo.” <i>European Journal of Cell Biology</i>, vol. 91, no. 11–12, Elsevier, 2012, pp. 923–29, doi:<a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">10.1016/j.ejcb.2012.04.002</a>."},"quality_controlled":"1","type":"journal_article","pmid":1,"department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2012-11-01T00:00:00Z","day":"01","year":"2012","publisher":"Elsevier","_id":"3158","author":[{"full_name":"Schachtner, Hannah","last_name":"Schachtner","first_name":"Hannah"},{"last_name":"Li","full_name":"Li, Ang","first_name":"Ang"},{"first_name":"David","last_name":"Stevenson","full_name":"Stevenson, David"},{"first_name":"Simon","full_name":"Calaminus, Simon","last_name":"Calaminus"},{"first_name":"Steven","full_name":"Thomas, Steven","last_name":"Thomas"},{"first_name":"Steve","full_name":"Watson, Steve","last_name":"Watson"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"last_name":"Wedlich Söldner","full_name":"Wedlich Söldner, Roland","first_name":"Roland"},{"first_name":"Douglas","last_name":"Strathdee","full_name":"Strathdee, Douglas"},{"full_name":"Machesky, Laura","last_name":"Machesky","first_name":"Laura"}],"article_processing_charge":"No","status":"public","volume":91,"publist_id":"3534","date_created":"2018-12-11T12:01:44Z","page":"923 - 929","publication":"European Journal of Cell Biology","month":"11","isi":1,"intvolume":"        91","oa":1,"language":[{"iso":"eng"}],"oa_version":"Submitted Version","scopus_import":"1","date_updated":"2025-09-30T07:54:24Z","publication_status":"published","abstract":[{"lang":"eng","text":"We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable by traditional methods, so conditional activation of Lifeact allows the study of actin dynamics in these cells live. We studied melanoblasts in native skin explants from embryos, allowing the visualization of live actin dynamics during cytokinesis and migration. Our study revealed that melanoblasts lacking the small GTPase Rac1 show a delay in the formation of new pseudopodia following cytokinesis that accounts for the previously reported cytokinesis delay in these cells. Thus, through use of this mouse model, we were able to gain insights into the actin dynamics of cells that could only previously be studied using fixed specimens or following isolation from their native tissue environment."}],"title":"Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo","external_id":{"isi":["000311775000013"],"pmid":["22658956"]},"doi":"10.1016/j.ejcb.2012.04.002"},{"publication_status":"published","date_updated":"2025-05-20T07:14:51Z","language":[{"iso":"eng"}],"oa_version":"None","doi":"10.1126/science.336.6077.32","external_id":{"pmid":["22491839"]},"title":"NextGen speaks 13 ","popular_science":"1","month":"04","date_created":"2018-12-11T12:01:47Z","publication":"Science","page":"32-34","oa":1,"intvolume":"       336","article_processing_charge":"No","_id":"3167","author":[{"first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","full_name":"Weber, Michele","last_name":"Weber"}],"publisher":"American Association for the Advancement of Science","date_published":"2012-04-06T00:00:00Z","year":"2012","day":"06","department":[{"_id":"MiSi"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"3516","volume":336,"status":"public","issue":"6077","main_file_link":[{"url":"https://doi.org/10.1126/science.336.6077.32","open_access":"1"}],"pmid":1,"type":"journal_article","OA_type":"free access","article_type":"letter_note","citation":{"mla":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>, vol. 336, no. 6077, American Association for the Advancement of Science, 2012, pp. 32–34, doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>.","apa":"Weber, M. (2012). NextGen speaks 13 . <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>","ieee":"M. Weber, “NextGen speaks 13 ,” <i>Science</i>, vol. 336, no. 6077. American Association for the Advancement of Science, pp. 32–34, 2012.","short":"M. Weber, Science 336 (2012) 32–34.","chicago":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>.","ista":"Weber M. 2012. NextGen speaks 13 . Science. 336(6077), 32–34.","ama":"Weber M. NextGen speaks 13 . <i>Science</i>. 2012;336(6077):32-34. doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>"}},{"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","file":[{"content_type":"application/pdf","file_name":"2012_CellBiology_Sixt.pdf","checksum":"45c02be33ebd99fc3077d60b9c90bdfa","creator":"kschuh","file_id":"5957","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:46:36Z","date_created":"2019-02-12T09:03:09Z","file_size":986566}],"status":"public","file_date_updated":"2020-07-14T12:46:36Z","volume":197,"publist_id":"7314","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2012","day":"30","date_published":"2012-04-30T00:00:00Z","publisher":"Rockefeller University Press","author":[{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179"}],"_id":"506","article_processing_charge":"No","citation":{"mla":"Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.” <i>Journal of Cell Biology</i>, vol. 197, no. 3, Rockefeller University Press, 2012, pp. 347–49, doi:<a href=\"https://doi.org/10.1083/jcb.201204039\">10.1083/jcb.201204039</a>.","ieee":"M. K. Sixt, “Cell migration: Fibroblasts find a new way to get ahead,” <i>Journal of Cell Biology</i>, vol. 197, no. 3. Rockefeller University Press, pp. 347–349, 2012.","apa":"Sixt, M. K. (2012). Cell migration: Fibroblasts find a new way to get ahead. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201204039\">https://doi.org/10.1083/jcb.201204039</a>","chicago":"Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2012. <a href=\"https://doi.org/10.1083/jcb.201204039\">https://doi.org/10.1083/jcb.201204039</a>.","short":"M.K. Sixt, Journal of Cell Biology 197 (2012) 347–349.","ama":"Sixt MK. Cell migration: Fibroblasts find a new way to get ahead. <i>Journal of Cell Biology</i>. 2012;197(3):347-349. doi:<a href=\"https://doi.org/10.1083/jcb.201204039\">10.1083/jcb.201204039</a>","ista":"Sixt MK. 2012. Cell migration: Fibroblasts find a new way to get ahead. Journal of Cell Biology. 197(3), 347–349."},"article_type":"original","tmp":{"short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"type":"journal_article","quality_controlled":"1","has_accepted_license":"1","issue":"3","title":"Cell migration: Fibroblasts find a new way to get ahead","external_id":{"isi":["000303467800004"]},"doi":"10.1083/jcb.201204039","oa_version":"Published Version","language":[{"iso":"eng"}],"scopus_import":"1","date_updated":"2025-09-30T08:24:00Z","publication_status":"published","intvolume":"       197","oa":1,"page":"347 - 349","publication":"Journal of Cell Biology","date_created":"2018-12-11T11:46:51Z","ddc":["570"],"isi":1,"month":"04","corr_author":"1"},{"publication_status":"published","date_updated":"2025-09-30T09:21:31Z","scopus_import":"1","oa_version":"None","language":[{"iso":"eng"}],"doi":"10.2174/138920311798841753","external_id":{"isi":["000299672600005"]},"abstract":[{"text":"Diffusing membrane constituents are constantly exposed to a variety of forces that influence their stochastic path. Single molecule experiments allow for resolving trajectories at extremely high spatial and temporal accuracy, thereby offering insights into en route interactions of the tracer. In this review we discuss approaches to derive information about the underlying processes, based on single molecule tracking experiments. In particular, we focus on a new versatile way to analyze single molecule diffusion in the absence of a full analytical treatment. The method is based on comprehensive comparison of an experimental data set against the hypothetical outcome of multiple experiments performed on the computer. Since Monte Carlo simulations can be easily and rapidly performed even on state-of-the-art PCs, our method provides a simple way for testing various - even complicated - diffusion models. We describe the new method in detail, and show the applicability on two specific examples: firstly, kinetic rate constants can be derived for the transient interaction of mobile membrane proteins; secondly, residence time and corral size can be extracted for confined diffusion.","lang":"eng"}],"title":"What can we learn from single molecule trajectories?","isi":1,"month":"12","publication":"Current Protein & Peptide Science","page":"714 - 724","date_created":"2018-12-11T12:02:28Z","intvolume":"        12","_id":"3287","author":[{"last_name":"Ruprecht","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markus","full_name":"Axmann, Markus","last_name":"Axmann"},{"full_name":"Wieser, Stefan","last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","orcid":"0000-0002-2670-2217"},{"first_name":"Gerhard","last_name":"Schuetz","full_name":"Schuetz, Gerhard"}],"article_processing_charge":"No","publisher":"Bentham Science Publishers","year":"2011","day":"01","date_published":"2011-12-01T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"publist_id":"3358","volume":12,"status":"public","issue":"8","type":"journal_article","quality_controlled":"1","citation":{"ama":"Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. 2011;12(8):714-724. doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>","ista":"Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single molecule trajectories? Current Protein &#38; Peptide Science. 12(8), 714–724.","ieee":"V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from single molecule trajectories?,” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.","apa":"Ruprecht, V., Axmann, M., Wieser, S., &#38; Schuetz, G. (2011). What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>","short":"V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein &#38; Peptide Science 12 (2011) 714–724.","chicago":"Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers, 2011. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>.","mla":"Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8, Bentham Science Publishers, 2011, pp. 714–24, doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>."}},{"issue":"6","has_accepted_license":"1","type":"journal_article","quality_controlled":"1","citation":{"apa":"Sixt, M. K., &#38; Parent, C. (2011). Cells on the move in Philadelphia. <i>Molecular Biology and Evolution</i>. Oxford University Press. <a href=\"https://doi.org/10.1091/mbc.E10-12-0958\">https://doi.org/10.1091/mbc.E10-12-0958</a>","ieee":"M. K. Sixt and C. Parent, “Cells on the move in Philadelphia,” <i>Molecular Biology and Evolution</i>, vol. 22, no. 6. Oxford University Press, p. 724, 2011.","short":"M.K. Sixt, C. Parent, Molecular Biology and Evolution 22 (2011) 724.","chicago":"Sixt, Michael K, and Carole Parent. “Cells on the Move in Philadelphia.” <i>Molecular Biology and Evolution</i>. Oxford University Press, 2011. <a href=\"https://doi.org/10.1091/mbc.E10-12-0958\">https://doi.org/10.1091/mbc.E10-12-0958</a>.","ama":"Sixt MK, Parent C. Cells on the move in Philadelphia. <i>Molecular Biology and Evolution</i>. 2011;22(6):724. doi:<a href=\"https://doi.org/10.1091/mbc.E10-12-0958\">10.1091/mbc.E10-12-0958</a>","ista":"Sixt MK, Parent C. 2011. Cells on the move in Philadelphia. Molecular Biology and Evolution. 22(6), 724.","mla":"Sixt, Michael K., and Carole Parent. “Cells on the Move in Philadelphia.” <i>Molecular Biology and Evolution</i>, vol. 22, no. 6, Oxford University Press, 2011, p. 724, doi:<a href=\"https://doi.org/10.1091/mbc.E10-12-0958\">10.1091/mbc.E10-12-0958</a>."},"article_type":"original","tmp":{"short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"publisher":"Oxford University Press","author":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Carole","full_name":"Parent, Carole","last_name":"Parent"}],"_id":"3371","article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"year":"2011","day":"15","pubrep_id":"373","date_published":"2011-03-15T00:00:00Z","publist_id":"3238","file":[{"checksum":"3467986ab7a64e7694ffd1013b5d9da9","content_type":"application/pdf","file_name":"IST-2015-373-v1+1_Mol._Biol._Cell-2011-Sixt-724.pdf","creator":"system","file_id":"5283","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:46:11Z","file_size":105421,"date_created":"2018-12-12T10:17:29Z"}],"status":"public","volume":22,"file_date_updated":"2020-07-14T12:46:11Z","ddc":["570"],"isi":1,"month":"03","page":"724","publication":"Molecular Biology and Evolution","date_created":"2018-12-11T12:02:57Z","oa":1,"intvolume":"        22","scopus_import":"1","publication_status":"published","date_updated":"2025-09-30T08:58:51Z","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1091/mbc.E10-12-0958","abstract":[{"lang":"eng","text":"The Minisymposium “Cell Migration and Motility” was attended by approximately 500 visitors and covered a broad range of questions in the field using diverse model systems. Topics comprised actin dynamics, cell polarity, force transduction, signal transduction, bar- rier transmigration, and chemotactic guidance."}],"title":"Cells on the move in Philadelphia","external_id":{"isi":["000288368200010"]}},{"month":"07","isi":1,"corr_author":"1","date_created":"2018-12-11T12:03:02Z","page":"32 - 34","publication":"Immunology Letters","intvolume":"       138","scopus_import":"1","date_updated":"2025-09-30T08:47:13Z","publication_status":"published","language":[{"iso":"eng"}],"oa_version":"None","doi":"10.1016/j.imlet.2011.02.013","title":"Interstitial locomotion of leukocytes","external_id":{"isi":["000292714800011"]},"issue":"1","quality_controlled":"1","type":"journal_article","citation":{"mla":"Sixt, Michael K. “Interstitial Locomotion of Leukocytes.” <i>Immunology Letters</i>, vol. 138, no. 1, Elsevier, 2011, pp. 32–34, doi:<a href=\"https://doi.org/10.1016/j.imlet.2011.02.013\">10.1016/j.imlet.2011.02.013</a>.","ista":"Sixt MK. 2011. Interstitial locomotion of leukocytes. Immunology Letters. 138(1), 32–34.","ama":"Sixt MK. Interstitial locomotion of leukocytes. <i>Immunology Letters</i>. 2011;138(1):32-34. doi:<a href=\"https://doi.org/10.1016/j.imlet.2011.02.013\">10.1016/j.imlet.2011.02.013</a>","apa":"Sixt, M. K. (2011). Interstitial locomotion of leukocytes. <i>Immunology Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.imlet.2011.02.013\">https://doi.org/10.1016/j.imlet.2011.02.013</a>","ieee":"M. K. Sixt, “Interstitial locomotion of leukocytes,” <i>Immunology Letters</i>, vol. 138, no. 1. Elsevier, pp. 32–34, 2011.","short":"M.K. Sixt, Immunology Letters 138 (2011) 32–34.","chicago":"Sixt, Michael K. “Interstitial Locomotion of Leukocytes.” <i>Immunology Letters</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.imlet.2011.02.013\">https://doi.org/10.1016/j.imlet.2011.02.013</a>."},"article_type":"review","publisher":"Elsevier","author":[{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179"}],"_id":"3385","article_processing_charge":"No","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2011-07-01T00:00:00Z","year":"2011","day":"01","publist_id":"3222","status":"public","volume":138},{"type":"journal_article","quality_controlled":"1","citation":{"short":"S. Soriano, M. Hons, K. Schumann, V. Kumar, T. Dennier, R. Lyck, M.K. Sixt, J. Stein, Journal of Immunology 187 (2011) 2356–2364.","chicago":"Soriano, Silvia, Miroslav Hons, Kathrin Schumann, Varsha Kumar, Timo Dennier, Ruth Lyck, Michael K Sixt, and Jens Stein. “In Vivo Analysis of Uropod Function during Physiological T Cell Trafficking.” <i>Journal of Immunology</i>. American Association of Immunologists, 2011. <a href=\"https://doi.org/10.4049/jimmunol.1100935\">https://doi.org/10.4049/jimmunol.1100935</a>.","ieee":"S. Soriano <i>et al.</i>, “In vivo analysis of uropod function during physiological T cell trafficking,” <i>Journal of Immunology</i>, vol. 187, no. 5. American Association of Immunologists, pp. 2356–2364, 2011.","apa":"Soriano, S., Hons, M., Schumann, K., Kumar, V., Dennier, T., Lyck, R., … Stein, J. (2011). In vivo analysis of uropod function during physiological T cell trafficking. <i>Journal of Immunology</i>. American Association of Immunologists. <a href=\"https://doi.org/10.4049/jimmunol.1100935\">https://doi.org/10.4049/jimmunol.1100935</a>","ama":"Soriano S, Hons M, Schumann K, et al. In vivo analysis of uropod function during physiological T cell trafficking. <i>Journal of Immunology</i>. 2011;187(5):2356-2364. doi:<a href=\"https://doi.org/10.4049/jimmunol.1100935\">10.4049/jimmunol.1100935</a>","ista":"Soriano S, Hons M, Schumann K, Kumar V, Dennier T, Lyck R, Sixt MK, Stein J. 2011. In vivo analysis of uropod function during physiological T cell trafficking. Journal of Immunology. 187(5), 2356–2364.","mla":"Soriano, Silvia, et al. “In Vivo Analysis of Uropod Function during Physiological T Cell Trafficking.” <i>Journal of Immunology</i>, vol. 187, no. 5, American Association of Immunologists, 2011, pp. 2356–64, doi:<a href=\"https://doi.org/10.4049/jimmunol.1100935\">10.4049/jimmunol.1100935</a>."},"article_type":"original","issue":"5","publication_identifier":{"issn":["0022-1767"],"eissn":["1550-6606"]},"publist_id":"3215","status":"public","volume":187,"publisher":"American Association of Immunologists","article_processing_charge":"No","_id":"3392","author":[{"last_name":"Soriano","full_name":"Soriano, Silvia","first_name":"Silvia"},{"orcid":"0000-0002-6625-3348","first_name":"Miroslav","last_name":"Hons","full_name":"Hons, Miroslav"},{"first_name":"Kathrin","last_name":"Schumann","full_name":"Schumann, Kathrin"},{"first_name":"Varsha","full_name":"Kumar, Varsha","last_name":"Kumar"},{"first_name":"Timo","last_name":"Dennier","full_name":"Dennier, Timo"},{"last_name":"Lyck","full_name":"Lyck, Ruth","first_name":"Ruth"},{"full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Jens","full_name":"Stein, Jens","last_name":"Stein"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"MiSi"}],"day":"01","year":"2011","date_published":"2011-09-01T00:00:00Z","intvolume":"       187","isi":1,"month":"09","publication":"Journal of Immunology","page":"2356 - 2364","date_created":"2018-12-11T12:03:04Z","doi":"10.4049/jimmunol.1100935","title":"In vivo analysis of uropod function during physiological T cell trafficking","abstract":[{"text":"Migrating lymphocytes acquire a polarized phenotype with a leading and a trailing edge, or uropod. Although in vitro experiments in cell lines or activated primary cell cultures have established that Rho-p160 coiled-coil kinase (ROCK)-myosin II-mediated uropod contractility is required for integrin de-adhesion on two-dimensional surfaces and nuclear propulsion through narrow pores in three-dimensional matrices, less is known about the role of these two events during the recirculation of primary, nonactivated lymphocytes. Using pharmacological antagonists of ROCK and myosin II, we report that inhibition of uropod contractility blocked integrin-independent mouse T cell migration through narrow, but not large, pores in vitro. T cell crawling on chemokine-coated endothelial cells under shear was severely impaired by ROCK inhibition, whereas transendothelial migration was only reduced through endothelial cells with high, but not low, barrier properties. Using three-dimensional thick-tissue imaging and dynamic two-photon microscopy of T cell motility in lymphoid tissue, we demonstrated a significant role for uropod contractility in intraluminal crawling and transendothelial migration through lymph node, but not bone marrow, endothelial cells. Finally, we demonstrated that ICAM-1, but not anatomical constraints or integrin-independent interactions, reduced parenchymal motility of inhibitor-treated T cells within the dense lymphoid microenvironment, thus assigning context-dependent roles for uropod contraction during lymphocyte recirculation.","lang":"eng"}],"external_id":{"isi":["000294059500040"]},"scopus_import":"1","publication_status":"published","date_updated":"2025-09-30T08:43:55Z","oa_version":"None","language":[{"iso":"eng"}]},{"month":"11","isi":1,"corr_author":"1","date_created":"2018-12-11T11:46:46Z","publication":"Science Signaling","intvolume":"         4","scopus_import":"1","date_updated":"2025-09-30T09:24:17Z","publication_status":"published","language":[{"iso":"eng"}],"oa_version":"None","doi":"10.1126/scisignal.2002617","title":"Setting the clock for recirculating lymphocytes","abstract":[{"lang":"eng","text":"In their search for antigens, lymphocytes continuously shuttle among blood vessels, lymph vessels, and lymphatic tissues. Chemokines mediate entry of lymphocytes into lymphatic tissues, and sphingosine 1-phosphate (S1P) promotes localization of lymphocytes to the vasculature. Both signals are sensed through G protein-coupled receptors (GPCRs). Most GPCRs undergo ligand-dependent homologous receptor desensitization, a process that decreases their signaling output after previous exposure to high ligand concentration. Such desensitization can explain why lymphocytes do not take an intermediate position between two signals but rather oscillate between them. The desensitization of S1P receptor 1 (S1PR1) is mediated by GPCR kinase 2 (GRK2). Deletion of GRK2 in lymphocytes compromises desensitization by high vascular S1P concentrations, thereby reducing responsiveness to the chemokine signal and trapping the cells in the vascular compartment. The desensitization kinetics of S1PR1 allows lymphocytes to dynamically shuttle between vasculature and lymphatic tissue, although the positional information in both compartments is static."}],"external_id":{"isi":["000296800500002"]},"article_number":"pe43","issue":"198","quality_controlled":"1","type":"journal_article","citation":{"ista":"Eichner A, Sixt MK. 2011. Setting the clock for recirculating lymphocytes. Science Signaling. 4(198), pe43.","ama":"Eichner A, Sixt MK. Setting the clock for recirculating lymphocytes. <i>Science Signaling</i>. 2011;4(198). doi:<a href=\"https://doi.org/10.1126/scisignal.2002617\">10.1126/scisignal.2002617</a>","short":"A. Eichner, M.K. Sixt, Science Signaling 4 (2011).","chicago":"Eichner, Alexander, and Michael K Sixt. “Setting the Clock for Recirculating Lymphocytes.” <i>Science Signaling</i>. American Association for the Advancement of Science, 2011. <a href=\"https://doi.org/10.1126/scisignal.2002617\">https://doi.org/10.1126/scisignal.2002617</a>.","apa":"Eichner, A., &#38; Sixt, M. K. (2011). Setting the clock for recirculating lymphocytes. <i>Science Signaling</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/scisignal.2002617\">https://doi.org/10.1126/scisignal.2002617</a>","ieee":"A. Eichner and M. K. Sixt, “Setting the clock for recirculating lymphocytes,” <i>Science Signaling</i>, vol. 4, no. 198. American Association for the Advancement of Science, 2011.","mla":"Eichner, Alexander, and Michael K. Sixt. “Setting the Clock for Recirculating Lymphocytes.” <i>Science Signaling</i>, vol. 4, no. 198, pe43, American Association for the Advancement of Science, 2011, doi:<a href=\"https://doi.org/10.1126/scisignal.2002617\">10.1126/scisignal.2002617</a>."},"publisher":"American Association for the Advancement of Science","author":[{"last_name":"Eichner","full_name":"Eichner, Alexander","id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"_id":"491","article_processing_charge":"No","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2011-11-08T00:00:00Z","year":"2011","day":"08","publist_id":"7329","status":"public","volume":4},{"isi":1,"month":"10","page":"4309 - 4322","publication":"EMBO Journal","date_created":"2018-12-11T11:46:55Z","oa":1,"intvolume":"        30","scopus_import":"1","date_updated":"2025-09-30T09:23:51Z","publication_status":"published","oa_version":"Submitted Version","language":[{"iso":"eng"}],"doi":"10.1038/emboj.2011.301","abstract":[{"lang":"eng","text":"Cancer stem cells or cancer initiating cells are believed to contribute to cancer recurrence after therapy. MicroRNAs (miRNAs) are short RNA molecules with fundamental roles in gene regulation. The role of miRNAs in cancer stem cells is only poorly understood. Here, we report miRNA expression profiles of glioblastoma stem cell-containing CD133 + cell populations. We find that miR-9, miR-9 * (referred to as miR-9/9 *), miR-17 and miR-106b are highly abundant in CD133 + cells. Furthermore, inhibition of miR-9/9 * or miR-17 leads to reduced neurosphere formation and stimulates cell differentiation. Calmodulin-binding transcription activator 1 (CAMTA1) is a putative transcription factor, which induces the expression of the anti-proliferative cardiac hormone natriuretic peptide A (NPPA). We identify CAMTA1 as an miR-9/9 * and miR-17 target. CAMTA1 expression leads to reduced neurosphere formation and tumour growth in nude mice, suggesting that CAMTA1 can function as tumour suppressor. Consistently, CAMTA1 and NPPA expression correlate with patient survival. Our findings could provide a basis for novel strategies of glioblastoma therapy."}],"title":"CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma stem cells","external_id":{"pmid":["21857646"],"isi":["000296715800018"]},"issue":"20","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199389/"}],"type":"journal_article","quality_controlled":"1","pmid":1,"citation":{"mla":"Schraivogel, Daniel, et al. “CAMTA1 Is a Novel Tumour Suppressor Regulated by MiR-9/9 * in Glioblastoma Stem Cells.” <i>EMBO Journal</i>, vol. 30, no. 20, Wiley-Blackwell, 2011, pp. 4309–22, doi:<a href=\"https://doi.org/10.1038/emboj.2011.301\">10.1038/emboj.2011.301</a>.","apa":"Schraivogel, D., Weinmann, L., Beier, D., Tabatabai, G., Eichner, A., Zhu, J., … Meister, G. (2011). CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma stem cells. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2011.301\">https://doi.org/10.1038/emboj.2011.301</a>","ieee":"D. Schraivogel <i>et al.</i>, “CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma stem cells,” <i>EMBO Journal</i>, vol. 30, no. 20. Wiley-Blackwell, pp. 4309–4322, 2011.","short":"D. Schraivogel, L. Weinmann, D. Beier, G. Tabatabai, A. Eichner, J. Zhu, M. Anton, M.K. Sixt, M. Weller, C. Beier, G. Meister, EMBO Journal 30 (2011) 4309–4322.","chicago":"Schraivogel, Daniel, Lasse Weinmann, Dagmar Beier, Ghazaleh Tabatabai, Alexander Eichner, Jia Zhu, Martina Anton, et al. “CAMTA1 Is a Novel Tumour Suppressor Regulated by MiR-9/9 * in Glioblastoma Stem Cells.” <i>EMBO Journal</i>. Wiley-Blackwell, 2011. <a href=\"https://doi.org/10.1038/emboj.2011.301\">https://doi.org/10.1038/emboj.2011.301</a>.","ama":"Schraivogel D, Weinmann L, Beier D, et al. CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma stem cells. <i>EMBO Journal</i>. 2011;30(20):4309-4322. doi:<a href=\"https://doi.org/10.1038/emboj.2011.301\">10.1038/emboj.2011.301</a>","ista":"Schraivogel D, Weinmann L, Beier D, Tabatabai G, Eichner A, Zhu J, Anton M, Sixt MK, Weller M, Beier C, Meister G. 2011. CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma stem cells. EMBO Journal. 30(20), 4309–4322."},"article_type":"original","publisher":"Wiley-Blackwell","_id":"518","author":[{"last_name":"Schraivogel","full_name":"Schraivogel, Daniel","first_name":"Daniel"},{"first_name":"Lasse","last_name":"Weinmann","full_name":"Weinmann, Lasse"},{"full_name":"Beier, Dagmar","last_name":"Beier","first_name":"Dagmar"},{"first_name":"Ghazaleh","full_name":"Tabatabai, Ghazaleh","last_name":"Tabatabai"},{"full_name":"Eichner, Alexander","last_name":"Eichner","first_name":"Alexander","id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jia","last_name":"Zhu","full_name":"Zhu, Jia"},{"first_name":"Martina","full_name":"Anton, Martina","last_name":"Anton"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Weller","full_name":"Weller, Michael","first_name":"Michael"},{"first_name":"Christoph","full_name":"Beier, Christoph","last_name":"Beier"},{"first_name":"Gunter","last_name":"Meister","full_name":"Meister, Gunter"}],"article_processing_charge":"No","department":[{"_id":"MiSi"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"19","year":"2011","date_published":"2011-10-19T00:00:00Z","publist_id":"7301","status":"public","volume":30},{"month":"05","corr_author":"1","publication":"Cell Migration","page":"149 - 165","date_created":"2018-12-11T12:03:41Z","oa":1,"intvolume":"       769","scopus_import":"1","date_updated":"2024-10-21T06:03:02Z","publication_status":"published","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1007/978-1-61779-207-6_11","abstract":[{"lang":"eng","text":"Cell migration on two-dimensional (2D) substrates follows entirely different rules than cell migration in three-dimensional (3D) environments. This is especially relevant for leukocytes that are able to migrate in the absence of adhesion receptors within the confined geometry of artificial 3D extracellular matrix scaffolds and within the interstitial space in vivo. Here, we describe in detail a simple and economical protocol to visualize dendritic cell migration in 3D collagen scaffolds along chemotactic gradients. This method can be adapted to other cell types and may serve as a physiologically relevant paradigm for the directed locomotion of most amoeboid cells."}],"title":"In vitro analysis of chemotactic leukocyte migration in 3D environments","main_file_link":[{"url":"https://pure.mpg.de/pubman/item/item_3219628_1/component/file_3219630/Sixt%20et%20al..pdf","open_access":"1"}],"type":"journal_article","quality_controlled":"1","citation":{"ista":"Sixt MK, Lämmermann T. 2011. In vitro analysis of chemotactic leukocyte migration in 3D environments. Cell Migration. 769, 149–165.","ama":"Sixt MK, Lämmermann T. In vitro analysis of chemotactic leukocyte migration in 3D environments. <i>Cell Migration</i>. 2011;769:149-165. doi:<a href=\"https://doi.org/10.1007/978-1-61779-207-6_11\">10.1007/978-1-61779-207-6_11</a>","ieee":"M. K. Sixt and T. Lämmermann, “In vitro analysis of chemotactic leukocyte migration in 3D environments,” <i>Cell Migration</i>, vol. 769. Springer, pp. 149–165, 2011.","apa":"Sixt, M. K., &#38; Lämmermann, T. (2011). In vitro analysis of chemotactic leukocyte migration in 3D environments. <i>Cell Migration</i>. Springer. <a href=\"https://doi.org/10.1007/978-1-61779-207-6_11\">https://doi.org/10.1007/978-1-61779-207-6_11</a>","chicago":"Sixt, Michael K, and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.” <i>Cell Migration</i>. Springer, 2011. <a href=\"https://doi.org/10.1007/978-1-61779-207-6_11\">https://doi.org/10.1007/978-1-61779-207-6_11</a>.","short":"M.K. Sixt, T. Lämmermann, Cell Migration 769 (2011) 149–165.","mla":"Sixt, Michael K., and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.” <i>Cell Migration</i>, vol. 769, Springer, 2011, pp. 149–65, doi:<a href=\"https://doi.org/10.1007/978-1-61779-207-6_11\">10.1007/978-1-61779-207-6_11</a>."},"article_type":"original","publisher":"Springer","alternative_title":["Methods in Molecular Biology"],"article_processing_charge":"No","_id":"3505","author":[{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"full_name":"Lämmermann, Tim","last_name":"Lämmermann","first_name":"Tim"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"day":"17","year":"2011","date_published":"2011-05-17T00:00:00Z","publist_id":"2882","status":"public","volume":769},{"publication_identifier":{"issn":["2663-337X"]},"has_accepted_license":"1","citation":{"ieee":"K. Schumann, “The role of chemotactic gradients in dendritic cell migration,” Institute of Science and Technology Austria, 2011.","apa":"Schumann, K. (2011). <i>The role of chemotactic gradients in dendritic cell migration</i>. Institute of Science and Technology Austria.","chicago":"Schumann, Kathrin. “The Role of Chemotactic Gradients in Dendritic Cell Migration.” Institute of Science and Technology Austria, 2011.","short":"K. Schumann, The Role of Chemotactic Gradients in Dendritic Cell Migration, Institute of Science and Technology Austria, 2011.","ama":"Schumann K. The role of chemotactic gradients in dendritic cell migration. 2011.","ista":"Schumann K. 2011. The role of chemotactic gradients in dendritic cell migration. Institute of Science and Technology Austria.","mla":"Schumann, Kathrin. <i>The Role of Chemotactic Gradients in Dendritic Cell Migration</i>. Institute of Science and Technology Austria, 2011."},"type":"dissertation","department":[{"_id":"MiSi"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","day":"01","year":"2011","pubrep_id":"11","date_published":"2011-03-01T00:00:00Z","publisher":"Institute of Science and Technology Austria","alternative_title":["ISTA Thesis"],"article_processing_charge":"No","_id":"3275","author":[{"last_name":"Schumann","full_name":"Schumann, Kathrin","id":"F44D762E-4F9D-11E9-B64C-9EB26CEFFB5F","first_name":"Kathrin"}],"file":[{"content_type":"application/pdf","file_name":"2011_Thesis_Kathrin_Schumann.pdf","checksum":"e69eee6252660f0b694a2ea8923ddc72","creator":"dernst","file_id":"6177","relation":"main_file","access_level":"closed","date_updated":"2020-07-14T12:46:06Z","date_created":"2019-03-26T08:12:21Z","file_size":4487708},{"file_id":"9175","relation":"main_file","content_type":"application/pdf","file_name":"2011_Thesis_Schumann_noS.pdf","checksum":"71727d63f424b5b446f68f4b87ecadc0","success":1,"creator":"dernst","file_size":4313127,"date_created":"2021-02-22T11:24:30Z","access_level":"open_access","date_updated":"2021-02-22T11:24:30Z"}],"status":"public","file_date_updated":"2021-02-22T11:24:30Z","acknowledgement":"I would like to express my sincere gratitude to the following people who made with their continuous support and encouragement this thesis possible: First, I want to thank Prof. Dr. Michael Sixt for his excellent supervision and mentoring, especially for the nice, relaxed working atmosphere, a lot of brilliant ideas and the freedom to work in my own way.\r\n\r\nProf. Dr. Reinhard Fässler for his constant support of the Sixt lab and for providing excellent working conditions. \r\n\r\nProf. Dr. Sanjiv Luther and Prof. Dr. Tobias Bollenbach for agreeing to be member of my thesis committee and to evaluate my work.\r\n\r\nDr. Walther Göhring, Carmen Schmitz, the Recombinant Protein Production core facility and the animal care takers for providing the “infrastructure” for this thesis. \r\n\r\nProf. Dr. Daniel Legler, Markus Bruckner and Dr. Julien Polleux for very fruitful collaborations and discussions.\r\n\r\nMy labmates for their help, a lot of discussions and to make the Sixt lab to a convenient place to work : Karin Hirsch, Tim Lämmeramnn, Holger Pflicke, Jörg Renkawitz, Michele Weber and Alexander Eichner All members of the Department of Molecular Medicine for their help. Especially I want to thank Sarah Schmidt, Karin Hirsch and Raphael Ruppert for their friendship, nice chats and their uncensored point of view. ","publist_id":"3371","page":"141","date_created":"2018-12-11T12:02:24Z","ddc":["570","579"],"month":"03","supervisor":[{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K"}],"corr_author":"1","oa":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2026-04-09T14:36:24Z","degree_awarded":"PhD","title":"The role of chemotactic gradients in dendritic cell migration","abstract":[{"text":"Chemokines organize immune cell trafficking by inducing either directed (tactic) or random (kinetic) migration and by activating integrins in order to support surface adhesion (haptic). Beyond that the same chemokines can establish clearly defined functional areas in secondary lymphoid organs. Until now it is unclear how chemokines can fulfill such diverse functions. One decisive prerequisite to explain these capacities is to know how chemokines are presented in tissue. In theory chemokines could occur either soluble or immobilized, and could be distributed either homogenously or as a concentration gradient. To dissect if and how the presenting mode of chemokines influences immune cells, I tested the response of dendritic cells (DCs) to differentially displayed chemokines. DCs are antigen presenting cells that reside in the periphery and migrate into draining lymph nodes (LNs) once exposed to inflammatory stimuli to activate naïve T cells. DCs are guided to and within the LN by the chemokine receptor CCR7, which has two ligands, the chemokines CCL19 and CCL21. Both CCR7 ligands are expressed by fibroblastic reticular cells in the LN, but differ in their ability to bind to heparan sulfate residues. CCL21 has a highly charged C-terminal extension, which mediates binding to anionic surfaces, whereas CCL19 is lacking such residues and likely distributes as a soluble molecule. This study shows that surface-bound CCL21 causes random, haptokinetic DC motility, which is confined to the chemokine coated area by insideout activation of β2 integrins that mediate cell binding to the surface. CCL19 on the other hand forms concentration gradients which trigger directional, chemotactic movement, but no surface adhesion. In addition DCs can actively manipulate this system by recruiting and activating serine proteases on their surfaces, which create - by proteolytically removing the adhesive C-terminus - a solubilized variant of CCL21 that functionally resembles CCL19. By generating a CCL21 concentration gradient DCs establish a positive feedback loop to recruit further DCs from the periphery to the CCL21 coated region. In addition DCs can sense chemotactic gradients as well as immobilized haptokinetic fields at the same time and integrate these signals. The result is chemotactically biased haptokinesis - directional migration confined to a chemokine coated track or area - which could explain the dynamic but spatially tightly controlled swarming leukocyte locomotion patterns that have been observed in lymphatic organs by intravital microscopists. The finding that DCs can approach soluble cues in a non-adhesive manner while they attach to surfaces coated with immobilized cues raises the question how these cells transmit intracellular forces to the environment, especially in the non-adherent migration mode. In order to migrate, cells have to generate and transmit force to the extracellular substrate. Force transmission is the prerequisite to procure an expansion of the leading edge and a forward motion of the whole cell body. In the current conceptions actin polymerization at the leading edge is coupled to extracellular ligands via the integrin family of transmembrane receptors, which allows the transmission of intracellular force. Against the paradigm of force transmission during migration, leukocytes, like DCs, are able to migrate in threedimensional environments without using integrin transmembrane receptors (Lämmermann et al., 2008). This reflects the biological function of leukocytes, as they can invade almost all tissues, whereby their migration has to be independent from the extracellular environment. How the cells can achieve this is unclear. For this study I examined DC migration in a defined threedimensional environment and highlighted actin-dynamics with the probe Lifeact-GFP. The result was that chemotactic DCs can switch between integrin-dependent and integrin- independent locomotion and can thereby adapt to the adhesive properties of their environment. If the cells are able to couple their actin cytoskeleton to the substrate, actin polymerization is entirely converted into protrusion. Without coupling the actin cortex undergoes slippage and retrograde actin flow can be observed. But retrograde actin flow can be completely compensated by higher actin polymerization rate keeping the migration velocity and the shape of the cells unaltered. Mesenchymal cells like fibroblast cannot balance the loss of adhesive interaction, cannot protrude into open space and, therefore, strictly depend on integrinmediated force coupling. This leukocyte specific phenomenon of “adaptive force transmission” endows these cells with the unique ability to transit and invade almost every type of tissue. ","lang":"eng"}],"OA_place":"publisher"}]