[{"status":"public","intvolume":" 39","external_id":{"isi":["001292894900001"],"pmid":["39157943"]},"author":[{"full_name":"Pan, Yi","first_name":"Yi","last_name":"Pan"},{"last_name":"Hochgerner","full_name":"Hochgerner, Mathias","first_name":"Mathias"},{"id":"d63197a3-c188-11ed-9387-8d33a3f13871","full_name":"Cichon, Malgorzata Anna","first_name":"Malgorzata Anna","last_name":"Cichon"},{"last_name":"Benezeder","full_name":"Benezeder, Theresa","first_name":"Theresa"},{"first_name":"Thomas","full_name":"Bieber, Thomas","last_name":"Bieber"},{"first_name":"Peter","full_name":"Wolf, Peter","last_name":"Wolf"}],"language":[{"iso":"eng"}],"title":"Langerhans cells: Central players in the pathophysiology of atopic dermatitis","quality_controlled":"1","license":"https://creativecommons.org/licenses/by/4.0/","page":"278-289","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":39,"OA_type":"hybrid","has_accepted_license":"1","ddc":["570"],"date_created":"2024-08-25T22:01:07Z","article_type":"original","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"This work was supported by the CK-CARE of the KühneFoundation, Switzerland; the China Scholarship Counciland Shanghai Biocelline Enterprise Co. Ltd, China.","publication_identifier":{"eissn":["1468-3083"],"issn":["0926-9959"]},"doi":"10.1111/jdv.20291","department":[{"_id":"MiSi"}],"citation":{"chicago":"Pan, Yi, Mathias Hochgerner, Malgorzata Anna Cichon, Theresa Benezeder, Thomas Bieber, and Peter Wolf. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” Journal of the European Academy of Dermatology and Venereology. Wiley, 2025. https://doi.org/10.1111/jdv.20291.","ieee":"Y. Pan, M. Hochgerner, M. A. Cichon, T. Benezeder, T. Bieber, and P. Wolf, “Langerhans cells: Central players in the pathophysiology of atopic dermatitis,” Journal of the European Academy of Dermatology and Venereology, vol. 39, no. 2. Wiley, pp. 278–289, 2025.","ista":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. 2025. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. Journal of the European Academy of Dermatology and Venereology. 39(2), 278–289.","apa":"Pan, Y., Hochgerner, M., Cichon, M. A., Benezeder, T., Bieber, T., & Wolf, P. (2025). Langerhans cells: Central players in the pathophysiology of atopic dermatitis. Journal of the European Academy of Dermatology and Venereology. Wiley. https://doi.org/10.1111/jdv.20291","short":"Y. Pan, M. Hochgerner, M.A. Cichon, T. Benezeder, T. Bieber, P. Wolf, Journal of the European Academy of Dermatology and Venereology 39 (2025) 278–289.","mla":"Pan, Yi, et al. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” Journal of the European Academy of Dermatology and Venereology, vol. 39, no. 2, Wiley, 2025, pp. 278–89, doi:10.1111/jdv.20291.","ama":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. Journal of the European Academy of Dermatology and Venereology. 2025;39(2):278-289. doi:10.1111/jdv.20291"},"type":"journal_article","oa":1,"publication":"Journal of the European Academy of Dermatology and Venereology","issue":"2","file_date_updated":"2025-04-16T09:59:37Z","_id":"17459","day":"01","month":"02","isi":1,"date_updated":"2025-05-19T13:58:50Z","abstract":[{"lang":"eng","text":"Atopic dermatitis (AD) is the most common chronic inflammatory skin disease worldwide. AD is a highly complex disease with different subtypes. Many elements of AD pathophysiology have been described, but if/how they interact with each other or which mechanisms are important in which patients is still unclear. Langerhans cells (LCs) are antigen-presenting cells (APCs) in the epidermis. Depending on the context, they can act either pro- or anti-inflammatory. Many different studies have investigated LCs in the context of AD and found them to be connected to all major mechanisms of AD pathophysiology. As APCs, LCs recruit other immune cells and shape the immune response, especially adaptive immunity via polarization of T cells. As sentinel cells, LCs are primary sensors of the skin microbiome and are important for the decision of immunity versus tolerance. LCs are also involved with the integrity of the skin barrier by influencing tight junctions. Finally, LCs are important cells in the neuro-immune crosstalk in the skin. In this review, we provide an overview about the many different roles of LCs in AD. Understanding LCs might bring us closer to a more complete understanding of this highly complex disease. Potentially, modulating LCs might offer new options for targeted therapies for AD patients."}],"year":"2025","scopus_import":"1","publisher":"Wiley","date_published":"2025-02-01T00:00:00Z","article_processing_charge":"Yes (in subscription journal)","OA_place":"publisher","publication_status":"published","oa_version":"Published Version","file":[{"content_type":"application/pdf","checksum":"12555ddb3490daf10b8d44e334e7312e","file_name":"2025_JEADV_Pan.pdf","file_size":457698,"success":1,"date_updated":"2025-04-16T09:59:37Z","file_id":"19583","creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2025-04-16T09:59:37Z"}]},{"scopus_import":"1","year":"2025","abstract":[{"lang":"eng","text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence."}],"isi":1,"month":"08","date_updated":"2026-04-28T13:26:50Z","publisher":"Springer Nature","date_published":"2025-08-01T00:00:00Z","OA_place":"publisher","publication_status":"published","article_processing_charge":"Yes (via OA deal)","project":[{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"},{"grant_number":"944-2020","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration"}],"file":[{"file_name":"2025_NatureImmunology_ReisRodrigues.pdf","checksum":"0c725123dca7797c682609bff2c4c5ac","content_type":"application/pdf","date_updated":"2025-07-31T08:00:33Z","success":1,"file_size":13514646,"file_id":"20096","date_created":"2025-07-31T08:00:33Z","relation":"main_file","access_level":"open_access","creator":"dernst"}],"oa_version":"Published Version","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"doi":"10.1038/s41590-025-02211-w","oa":1,"type":"journal_article","citation":{"chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology. Springer Nature, 2025. https://doi.org/10.1038/s41590-025-02211-w.","ieee":"P. Dos Reis Rodrigues et al., “Migrating immune cells globally coordinate protrusive forces,” Nature Immunology, vol. 26. Springer Nature, pp. 1258–1266, 2025.","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-025-02211-w","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:10.1038/s41590-025-02211-w.","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 2025;26:1258–1266. doi:10.1038/s41590-025-02211-w","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266."},"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"file_date_updated":"2025-07-31T08:00:33Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication":"Nature Immunology","day":"01","_id":"20082","related_material":{"record":[{"id":"20149","relation":"dissertation_contains","status":"public"}],"link":[{"url":"https://ista.ac.at/en/news/bench-pressing-cells/","relation":"press_release","description":"News on ISTA website"}]},"volume":26,"has_accepted_license":"1","OA_type":"hybrid","article_type":"letter_note","corr_author":"1","ddc":["570"],"date_created":"2025-07-27T22:01:26Z","PlanS_conform":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","pmid":1,"intvolume":" 26","status":"public","author":[{"first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia","orcid":"0000-0003-1681-508X","last_name":"Dos Reis Rodrigues","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F"},{"full_name":"Avellaneda Sarrió, Mario","first_name":"Mario","orcid":"0000-0001-6406-524X","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"id":"3795523E-F248-11E8-B48F-1D18A9856A87","last_name":"Canigova","orcid":"0000-0002-8518-5926","first_name":"Nikola","full_name":"Canigova, Nikola"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","full_name":"Gärtner, Florian R","last_name":"Gärtner","orcid":"0000-0001-6120-3723"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518","first_name":"Kari","full_name":"Vaahtomeri, Kari"},{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","first_name":"Michael","full_name":"Riedl, Michael","last_name":"Riedl","orcid":"0000-0003-4844-6311"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries","full_name":"De Vries, Ingrid","first_name":"Ingrid"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert"},{"full_name":"Fukui, Yoshinori","first_name":"Yoshinori","last_name":"Fukui"},{"first_name":"Alba","full_name":"Juanes Garcia, Alba","orcid":"0000-0002-1009-9652","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt"}],"external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"language":[{"iso":"eng"}],"title":"Migrating immune cells globally coordinate protrusive forces","quality_controlled":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"page":"1258–1266"},{"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"10703"},{"id":"20082","status":"public","relation":"part_of_dissertation"}]},"has_accepted_license":"1","corr_author":"1","ddc":["570"],"date_created":"2025-08-08T09:18:02Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K"}],"status":"public","author":[{"last_name":"Dos Reis Rodrigues","orcid":"0000-0003-1681-508X","first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F"}],"language":[{"iso":"eng"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","title":"Coordination of protrusive forces in immune cell migration ","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"page":"114","year":"2025","abstract":[{"lang":"eng","text":"Immune responses depend on the coordinated and efficient migration of leukocytes. These\r\ncells, which are embedded and tightly confined within tissues, must navigate and traverse\r\ndiverse and complex three-dimensional environments. Leukocytes adapt their locomotory\r\nbehavior to the mechanical, geometrical, and biochemical characteristics of their\r\nsurroundings. In low-density environments, where the pore size of the interstitial matrix\r\nallows free passage, these cells position the nucleus directly behind the lamellipodium, the\r\nprotrusive actin structure that forms the leading front of the cell. In this configuration, they\r\nuse the nucleus as a gauge to identify the path of least resistance.\r\nHere, we show that in high-density environments, where the pore size precludes free passage\r\nof the cell body, leukocytes reposition the microtubule-organizing center (MTOC) and\r\nassociated organelles in front of the nucleus. In this configuration, they use actin structures\r\nprotruding orthogonally to the direction of migration in order to open a path for the cell body.\r\nWe identify two distinct actin populations that serve this purpose at different subcellular\r\nlocalizations. At the leading edge, local indentation of the plasma membrane leads to\r\nrecruitment of the Wiskott-Aldrich syndrome protein (WASp), which, via Arp2/3, results in\r\nthe formation of individual actin foci. At the cell body, actin polymerization is triggered by\r\nDOCK8, a Cdc42 exchange factor, resulting in the formation of a central actin pool.\r\nWe demonstrate that the central and peripheral actin pools are functionally communicating\r\nand that depletion of the central actin pool leads to increased actin accumulation at the cell\r\nfront, resulting in excessive extension of the leading edge."}],"alternative_title":["ISTA Thesis"],"month":"08","date_updated":"2026-04-28T13:26:50Z","publisher":"Institute of Science and Technology Austria","date_published":"2025-08-08T00:00:00Z","OA_place":"publisher","publication_status":"published","article_processing_charge":"No","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"}],"oa_version":"Published Version","file":[{"date_created":"2025-08-27T12:59:10Z","relation":"main_file","access_level":"open_access","creator":"prodrigu","file_id":"20232","date_updated":"2025-08-27T12:59:10Z","success":1,"file_size":63885565,"file_name":"2025_ReisRodrigues_Patricia_Thesis.pdf","checksum":"fda8a1070667c3562263f4867609b41b","content_type":"application/pdf"},{"file_id":"20233","creator":"prodrigu","access_level":"closed","relation":"source_file","date_created":"2025-08-27T13:00:30Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"e8b65affcbce846a926454df4b2867b9","file_name":"2025_ReisRodrigues_Patricia_Thesis.docx","file_size":50483434,"date_updated":"2025-08-27T13:02:28Z"}],"degree_awarded":"PhD","publication_identifier":{"issn":["2663-337X"]},"acknowledgement":"I would like to acknowledge the\r\nfinancial support of the European Research Council through the ERC-SyG grant “Pushing from\r\nwithin: Control of cell shape, integrity and motility by cytoskeletal pushing forces”\r\n(01071793), which made this research possible. ","doi":"10.15479/AT-ISTA-20149","oa":1,"department":[{"_id":"GradSch"},{"_id":"MiSi"}],"type":"dissertation","citation":{"ista":"Dos Reis Rodrigues P. 2025. Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria.","apa":"Dos Reis Rodrigues, P. (2025). Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20149","short":"P. Dos Reis Rodrigues, Coordination of Protrusive Forces in Immune Cell Migration , Institute of Science and Technology Austria, 2025.","mla":"Dos Reis Rodrigues, Patricia. Coordination of Protrusive Forces in Immune Cell Migration . Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20149.","ama":"Dos Reis Rodrigues P. Coordination of protrusive forces in immune cell migration . 2025. doi:10.15479/AT-ISTA-20149","chicago":"Dos Reis Rodrigues, Patricia. “Coordination of Protrusive Forces in Immune Cell Migration .” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20149.","ieee":"P. Dos Reis Rodrigues, “Coordination of protrusive forces in immune cell migration ,” Institute of Science and Technology Austria, 2025."},"file_date_updated":"2025-08-27T13:02:28Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"NanoFab"}],"_id":"20149","day":"08"},{"publisher":"National Academy of Sciences","date_published":"2025-08-26T00:00:00Z","year":"2025","abstract":[{"text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations.","lang":"eng"}],"scopus_import":"1","isi":1,"month":"08","date_updated":"2026-05-20T08:59:54Z","file":[{"file_size":16069140,"success":1,"date_updated":"2025-09-08T07:23:29Z","content_type":"application/pdf","checksum":"b36abd92673b6d76376fc9434bad52cc","file_name":"2025_PNAS_Ucar.pdf","creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2025-09-08T07:23:29Z","file_id":"20307"}],"oa_version":"Published Version","publication_status":"published","OA_place":"publisher","article_processing_charge":"Yes (in subscription journal)","project":[{"call_identifier":"H2020","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"oa":1,"department":[{"_id":"EdHa"},{"_id":"MiSi"}],"citation":{"apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., & Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2504064122","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 2025;122(34). doi:10.1073/pnas.2504064122","mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings of the National Academy of Sciences, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:10.1073/pnas.2504064122.","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2504064122.","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” Proceedings of the National Academy of Sciences, vol. 122, no. 34. National Academy of Sciences, 2025."},"type":"journal_article","acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"doi":"10.1073/pnas.2504064122","day":"26","_id":"20289","issue":"34","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"file_date_updated":"2025-09-08T07:23:29Z","publication":"Proceedings of the National Academy of Sciences","has_accepted_license":"1","OA_type":"hybrid","related_material":{"link":[{"relation":"software","url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis"}]},"volume":122,"ec_funded":1,"PlanS_conform":"1","article_number":"e2504064122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"article_type":"original","corr_author":"1","ddc":["570"],"date_created":"2025-09-07T22:01:32Z","author":[{"orcid":"0000-0003-0506-4217","last_name":"Ucar","full_name":"Ucar, Mehmet C","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"first_name":"Alsberga","full_name":"Zane, Alsberga","orcid":"0009-0003-0415-7603","last_name":"Zane","id":"60f7509a-f652-11ea-9d86-b963d6490d7c"},{"orcid":"0000-0002-7698-3061","last_name":"Alanko","full_name":"Alanko, Jonna H","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"APC_amount":"5766,07 EUR","external_id":{"pmid":["40838890"],"isi":["001562181600001"]},"language":[{"iso":"eng"}],"intvolume":" 122","status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"Self-generated chemotaxis of mixed cell populations","quality_controlled":"1"},{"article_processing_charge":"No","project":[{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793"},{"_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","name":"Motile active matter models of migrating cells and chiral filaments","grant_number":"26360"}],"OA_place":"repository","publication_status":"draft","oa_version":"Preprint","date_updated":"2026-06-10T09:41:11Z","month":"09","abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"year":"2025","date_published":"2025-09-25T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1101/2025.05.20.655037","open_access":"1"}],"publisher":"bioRxiv","_id":"21427","day":"25","doi":"10.1101/2025.05.20.655037","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"citation":{"chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” bioRxiv, n.d. https://doi.org/10.1101/2025.05.20.655037.","ieee":"Z. Dunajova et al., “Substrate heterogeneity promotes cancer cell dissemination through interface roughening.” bioRxiv.","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. 10.1101/2025.05.20.655037.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., & Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv. https://doi.org/10.1101/2025.05.20.655037","mla":"Dunajova, Zuzana, et al. Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening. bioRxiv, doi:10.1101/2025.05.20.655037.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. doi:10.1101/2025.05.20.655037","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, (n.d.)."},"type":"preprint","oa":1,"date_created":"2026-03-11T08:40:06Z","ddc":["539","570"],"corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","related_material":{"record":[{"id":"21423","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"research_data","id":"21439"}]},"has_accepted_license":"1","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"status":"public","language":[{"iso":"eng"}],"author":[{"last_name":"Dunajova","first_name":"Zuzana","full_name":"Dunajova, Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tasciyan","orcid":"0000-0003-1671-393X","first_name":"Saren","full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Juraj","full_name":"Majek, Juraj","last_name":"Majek","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sahai, Erik","first_name":"Erik","last_name":"Sahai"},{"full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"}]},{"OA_place":"publisher","publication_status":"published","project":[{"name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","call_identifier":"H2020"},{"call_identifier":"FWF","grant_number":"W01250-B20","name":"Nano-Analytics of Cellular Systems","_id":"265E2996-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","file":[{"file_id":"19748","date_created":"2025-05-28T07:38:17Z","relation":"source_file","access_level":"closed","creator":"cchlebak","file_name":"NikolaCanigova_Thesis_final.docx","checksum":"1a2d1525d19347fbb879ef57c02951bf","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2025-11-27T23:30:02Z","embargo_to":"open_access","file_size":103879193},{"file_size":194530600,"date_updated":"2025-11-27T23:30:02Z","content_type":"application/pdf","file_name":"NikolaCanigova_Thesis_final_PDFA2a_fixed.pdf","checksum":"c1d8f9a40a8e19fcf895373f4b773a46","access_level":"open_access","creator":"cchlebak","date_created":"2025-05-28T07:39:53Z","relation":"main_file","embargo":"2025-11-27","file_id":"19749"}],"oa_version":"Published Version","abstract":[{"text":"Cell migration is a crucial process in animal development and maintenance. It is incredibly\r\nheterogeneous, with different cell types utilizing fundamentally distinct migration strategies.\r\nThe strategies also depend on the cellular microenvironment, where cells can switch between\r\nmigration modes as they encounter new environmental cues. In this thesis, we investigated\r\nhow dendritic cells adapt their migration strategy when encountering geometrically,\r\nmechanically and chemically distinct environments.\r\nWhen dendritic cells are embedded in a homogeneous fibrous network, they migrate in a fast\r\nand directional amoeboid manner. In this migration strategy, extracellular proteolysis and\r\nintegrin-mediated adhesions are dispensable. Instead, the cells use topography of the\r\nenvironment to propel their cell body forward. To migrate efficiently in the maze of different\r\npore sizes, they position the nucleus ahead of the microtubule organizing center (MTOC) and\r\nuse it to gauge the pores to identify the path of least resistance. Our aim was to identify\r\nwhether dendritic cells adapt their migration strategy when encountering asymmetrical\r\ntransitions into much denser environments with limited choice of large pores. In such invasive\r\ntransitions it is unclear if the cells can cross tight pores without the use of adhesions and\r\nextracellular proteolysis and whether they maintain the nucleus in the cell front.\r\nUsing various cell migration assays such as fibrous 3D collagen gels, geometrically defined\r\nmicrochannels with constrictions and simplistic under agarose migration assay, we provide\r\na comprehensive characterization of invasive migration of dendritic cells. We show that\r\nduring invasion the cells stall and stretch, reflecting the difficulty to translocate the bulky cell\r\nbody into the dense environment. In collagen gels, we show that dendritic cells can invade\r\nwithout proteolysis and adhesions. Instead, they utilize contractility, which can lead to largescale collagen compressions. During invasion, the nucleus stalls at tight constrictions, leading\r\nto a transient organelle reorientation. To resolve the stalling, upregulated rear contractility is\r\nrequired. This contractile force is simultaneously necessary for reverting the nucleus back to\r\nthe cell front after invasion and maintaining this positioning during permissive migration.\r\nA functional role of the reorientation was uncovered in the first collaboration project.\r\nA prominent central actin pool was identified around the MTOC, especially pronounced in\r\ndense and compressive environments. The actin pool was shown to generate pushing forces\r\nto dilate the space for cell translocation. These forces are only necessary in non-permissive\r\nenvironments, where the nucleus reorients to the cell rear, allowing the actin pool to\r\ngenerate space. In permissive environments where space generation is dispensable, the\r\nMTOC is located behind the nucleus and the actin cloud has reduced intensity, allowing more\r\nactin to be incorporated into the lamellipodium, speeding up migration.\r\nIn the second collaboration project, we investigated the effects of distinct chemical\r\nenvironments on dendritic cell migration. The strikingly persistent migration of these cells\r\nwas explained by their ability to modulate and even self-generate chemokine gradients. This\r\nallows the cells to migrate faster and more persistent in uniform chemokine fields compared\r\nto imposed chemokine gradients. The chemokine receptor CCR7 was identified as a crucial\r\nplayer in this process, both sensing the signal and internalizing the chemokine to create a sink.","lang":"eng"}],"year":"2025","alternative_title":["ISTA Thesis"],"month":"05","date_updated":"2026-04-07T12:38:44Z","publisher":"Institute of Science and Technology Austria","date_published":"2025-05-27T00:00:00Z","file_date_updated":"2025-11-27T23:30:02Z","day":"27","_id":"19745","degree_awarded":"PhD","acknowledgement":"This project has received funding from the Austrian Science Fund (FWF) via the doctorate\r\ncollege DK NanoCell and from the European Union’s Horizon 2020 research and innovation\r\nprogramme under the Marie Skłodowska-Curie Grant Agreement No. 665385.\r\n","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-058-9"]},"doi":"10.15479/AT-ISTA-19745","OA_embargo":"6","oa":1,"department":[{"_id":"MiSi"},{"_id":"GradSch"}],"type":"dissertation","citation":{"chicago":"Canigova, Nikola. “Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19745.","ieee":"N. Canigova, “Adaptive strategies of dendritic cell migration in response to environmental cues,” Institute of Science and Technology Austria, 2025.","ista":"Canigova N. 2025. Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria.","apa":"Canigova, N. (2025). Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19745","mla":"Canigova, Nikola. Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19745.","ama":"Canigova N. Adaptive strategies of dendritic cell migration in response to environmental cues. 2025. doi:10.15479/AT-ISTA-19745","short":"N. Canigova, Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues, Institute of Science and Technology Austria, 2025."},"corr_author":"1","ddc":["570"],"date_created":"2025-05-26T08:49:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"14274"}]},"ec_funded":1,"has_accepted_license":"1","title":"Adaptive strategies of dendritic cell migration in response to environmental cues","page":"133","supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"}],"status":"public","author":[{"full_name":"Canigova, Nikola","first_name":"Nikola","orcid":"0000-0002-8518-5926","last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}]},{"month":"09","isi":1,"date_updated":"2025-09-08T09:50:13Z","scopus_import":"1","abstract":[{"lang":"eng","text":"Venous thromboembolism (VTE) is a common, deadly disease with an increasing incidence despite preventive efforts. Clinical observations have associated elevated antibody concentrations or antibody-based therapies with thrombotic events. However, how antibodies contribute to thrombosis is unknown. Here, we show that reduced blood flow enabled immunoglobulin M (IgM) to bind to FcμR and the polymeric immunoglobulin receptor (pIgR), initiating endothelial activation and platelet recruitment. Subsequently, the procoagulant surface of activated platelets accommodated antigen- and FcγR-independent IgG deposition. This leads to classical complement activation, setting in motion a prothrombotic vicious circle. Key elements of this mechanism were present in humans in the setting of venous stasis as well as in the dysregulated immunothrombosis of COVID-19. This antibody-driven thrombosis can be prevented by pharmacologically targeting complement. Hence, our results uncover antibodies as previously unrecognized central regulators of thrombosis. These findings carry relevance for therapeutic application of antibodies and open innovative avenues to target thrombosis without compromising hemostasis."}],"year":"2024","publisher":"Elsevier","date_published":"2024-09-10T00:00:00Z","article_processing_charge":"Yes (in subscription journal)","publication_status":"published","oa_version":"Published Version","file":[{"date_updated":"2024-09-30T09:16:03Z","success":1,"file_size":6892750,"checksum":"4683de43d06a8fd8e3fc91af4ddc1ba2","file_name":"2024_Immunity_Stark.pdf","content_type":"application/pdf","relation":"main_file","date_created":"2024-09-30T09:16:03Z","creator":"dernst","access_level":"open_access","file_id":"18162"}],"acknowledgement":"We thank Michael Carroll (Harvard Medical School, Boston) for providing Ighmtm1Che, C4−/−, and C3−/− mice; Mark Suter (University of Zurich, Zurich) for providing Aicda−/− mice; Marina Botto (Imperial College London, London) for providing C1q−/− and fB−/− mice; Craig Gerard (Harvard Medical School, Boston) for providing C3aR−/− mice; Falk Nimmerjahn (University Erlangen-Nuernberg, Erlangen) for providing Fcgr−/−Fcgr2b−/− mice; Karl Lang (University of Duisburg-Essen, Essen) for providing Fcmr−/− mice; Hans Hengartner and Rolf Zinkernagel (ETH Zurich, Zurich) for providing KL25 mice; Mark Zabel (University Hospital of Zurich, Zurich) for providing CR2−/− mice; Christie Ballantyne (Baylor College of Medicine, Houston) for providing CD11c−/− mice; and Siamon Gordon (University of Oxford, Oxford) for providing CD11b−/− mice. A.V. wishes to thank Michael Grünaug and dedicates this work to Annette, Rita, and Hans.\r\nThis project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. \r\n947611) (K.S.). This study was supported by the Deutsche Forschungsgemeinschaft through the collaborative research center 914 project B02 (K.S. and S.M.), project B04 (A.V.), project A01 (M.M.), project B01 (M.S.), the collaborative research center 1123 project B07 (K.S. and S.M.), the collaborative research center 359 (project A03 [K.S.] and B02 [M.S.]), the international research training group 1911 project B09 (A.V.), the clinical research unit 303 project 7 (A.V.), cluster of excellence 2167 (A.V.), collaborative research center 1526 project 05 (A.V.), the ANR-DFG project JAKPOT (K.S.), LMUexcellent (K.S.), and the Deutsche Zentrum für Herz-Kreislauf-Forschung (PostDoc Grant and partner site project [K.S. and S.M.]). M.I. is supported by the European Research Council (ERC) Advanced Grant 101141363, ERC Proof of Concept Grant 101138728, Italian Association for Cancer Research (AIRC) Grants 19891 and \r\n22737, Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and PRIN \r\n2022FMESXL, Funded Research Agreement from Asher Biotherapeutics, VIR Biotechnology, and BlueJay Therapeutics. V.F. is supported by the Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and Fondazione Prossimo Mio.","publication_identifier":{"eissn":["1097-4180"]},"doi":"10.1016/j.immuni.2024.08.007","department":[{"_id":"MiSi"}],"citation":{"ieee":"K. Stark et al., “Antibodies and complement are key drivers of thrombosis,” Immunity, vol. 57, no. 9. Elsevier, pp. 2140–2156, 2024.","chicago":"Stark, Konstantin, Badr Kilani, Sven Stockhausen, Johanna Busse, Irene Schubert, Thuy Duong Tran, Florian R Gärtner, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” Immunity. Elsevier, 2024. https://doi.org/10.1016/j.immuni.2024.08.007.","mla":"Stark, Konstantin, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” Immunity, vol. 57, no. 9, Elsevier, 2024, pp. 2140–56, doi:10.1016/j.immuni.2024.08.007.","ama":"Stark K, Kilani B, Stockhausen S, et al. Antibodies and complement are key drivers of thrombosis. Immunity. 2024;57(9):2140-2156. doi:10.1016/j.immuni.2024.08.007","short":"K. Stark, B. Kilani, S. Stockhausen, J. Busse, I. Schubert, T.D. Tran, F.R. Gärtner, A. Leunig, K. Pekayvaz, L. Nicolai, V. Fumagalli, J. Stermann, F. Stephan, C. David, M.B. Müller, B. Heyman, A. Lux, A. Da Palma Guerreiro, L.P. Frenzel, C.Q. Schmidt, A. Dopler, M. Moser, S. Chandraratne, M.L. Von Brühl, M. Lorenz, T. Korff, M. Rudelius, O. Popp, M. Kirchner, P. Mertins, F. Nimmerjahn, M. Iannacone, M. Sperandio, B. Engelmann, A. Verschoor, S. Massberg, Immunity 57 (2024) 2140–2156.","apa":"Stark, K., Kilani, B., Stockhausen, S., Busse, J., Schubert, I., Tran, T. D., … Massberg, S. (2024). Antibodies and complement are key drivers of thrombosis. Immunity. Elsevier. https://doi.org/10.1016/j.immuni.2024.08.007","ista":"Stark K, Kilani B, Stockhausen S, Busse J, Schubert I, Tran TD, Gärtner FR, Leunig A, Pekayvaz K, Nicolai L, Fumagalli V, Stermann J, Stephan F, David C, Müller MB, Heyman B, Lux A, Da Palma Guerreiro A, Frenzel LP, Schmidt CQ, Dopler A, Moser M, Chandraratne S, Von Brühl ML, Lorenz M, Korff T, Rudelius M, Popp O, Kirchner M, Mertins P, Nimmerjahn F, Iannacone M, Sperandio M, Engelmann B, Verschoor A, Massberg S. 2024. Antibodies and complement are key drivers of thrombosis. Immunity. 57(9), 2140–2156."},"type":"journal_article","oa":1,"publication":"Immunity","issue":"9","file_date_updated":"2024-09-30T09:16:03Z","_id":"18109","day":"10","volume":57,"has_accepted_license":"1","ddc":["570"],"date_created":"2024-09-22T22:01:42Z","article_type":"original","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","intvolume":" 57","external_id":{"isi":["001317438500001"],"pmid":["39226900"]},"author":[{"full_name":"Stark, Konstantin","first_name":"Konstantin","last_name":"Stark"},{"full_name":"Kilani, Badr","first_name":"Badr","last_name":"Kilani"},{"last_name":"Stockhausen","full_name":"Stockhausen, Sven","first_name":"Sven"},{"first_name":"Johanna","full_name":"Busse, Johanna","last_name":"Busse"},{"full_name":"Schubert, Irene","first_name":"Irene","last_name":"Schubert"},{"last_name":"Tran","first_name":"Thuy Duong","full_name":"Tran, Thuy Duong"},{"full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Leunig","full_name":"Leunig, Alexander","first_name":"Alexander"},{"full_name":"Pekayvaz, Kami","first_name":"Kami","last_name":"Pekayvaz"},{"first_name":"Leo","full_name":"Nicolai, Leo","last_name":"Nicolai"},{"full_name":"Fumagalli, Valeria","first_name":"Valeria","last_name":"Fumagalli"},{"last_name":"Stermann","first_name":"Julia","full_name":"Stermann, Julia"},{"full_name":"Stephan, Felix","first_name":"Felix","last_name":"Stephan"},{"last_name":"David","full_name":"David, Christian","first_name":"Christian"},{"last_name":"Müller","full_name":"Müller, Martin B.","first_name":"Martin B."},{"last_name":"Heyman","first_name":"Birgitta","full_name":"Heyman, Birgitta"},{"last_name":"Lux","first_name":"Anja","full_name":"Lux, Anja"},{"full_name":"Da Palma Guerreiro, Alexandra","first_name":"Alexandra","last_name":"Da Palma Guerreiro"},{"last_name":"Frenzel","full_name":"Frenzel, Lukas P.","first_name":"Lukas P."},{"first_name":"Christoph Q.","full_name":"Schmidt, Christoph Q.","last_name":"Schmidt"},{"last_name":"Dopler","first_name":"Arthur","full_name":"Dopler, Arthur"},{"last_name":"Moser","first_name":"Markus","full_name":"Moser, Markus"},{"last_name":"Chandraratne","first_name":"Sue","full_name":"Chandraratne, Sue"},{"full_name":"Von Brühl, Marie Luise","first_name":"Marie Luise","last_name":"Von Brühl"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"last_name":"Korff","full_name":"Korff, Thomas","first_name":"Thomas"},{"last_name":"Rudelius","full_name":"Rudelius, Martina","first_name":"Martina"},{"first_name":"Oliver","full_name":"Popp, Oliver","last_name":"Popp"},{"last_name":"Kirchner","full_name":"Kirchner, Marieluise","first_name":"Marieluise"},{"last_name":"Mertins","first_name":"Philipp","full_name":"Mertins, Philipp"},{"first_name":"Falk","full_name":"Nimmerjahn, Falk","last_name":"Nimmerjahn"},{"first_name":"Matteo","full_name":"Iannacone, Matteo","last_name":"Iannacone"},{"first_name":"Markus","full_name":"Sperandio, Markus","last_name":"Sperandio"},{"first_name":"Bernd","full_name":"Engelmann, Bernd","last_name":"Engelmann"},{"last_name":"Verschoor","first_name":"Admar","full_name":"Verschoor, Admar"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"}],"language":[{"iso":"eng"}],"title":"Antibodies and complement are key drivers of thrombosis","quality_controlled":"1","page":"2140-2156","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"publication_status":"published","article_processing_charge":"Yes (in subscription journal)","project":[{"grant_number":"I03601","call_identifier":"FWF","name":"Control of embryonic cleavage pattern","_id":"2646861A-B435-11E9-9278-68D0E5697425"}],"oa_version":"Published Version","file":[{"file_id":"17267","access_level":"open_access","creator":"dernst","date_created":"2024-07-16T12:12:43Z","relation":"main_file","content_type":"application/pdf","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","checksum":"7891ebe7c900ae47469ab127031dd1ec","file_size":9897883,"success":1,"date_updated":"2024-07-16T12:12:43Z"}],"abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}],"scopus_import":"1","year":"2024","date_updated":"2025-09-04T11:48:28Z","isi":1,"month":"02","date_published":"2024-02-01T00:00:00Z","publisher":"Springer Nature","file_date_updated":"2024-07-16T12:12:43Z","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"publication":"Nature Physics","day":"01","_id":"14846","doi":"10.1038/s41567-023-02302-1","acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"oa":1,"type":"journal_article","citation":{"ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02302-1","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 2024;20:310-321. doi:10.1038/s41567-023-02302-1","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics, vol. 20, Springer Nature, 2024, pp. 310–21, doi:10.1038/s41567-023-02302-1.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics. Springer Nature, 2024. https://doi.org/10.1038/s41567-023-02302-1.","ieee":"S. Caballero Mancebo et al., “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” Nature Physics, vol. 20. Springer Nature, pp. 310–321, 2024."},"department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"corr_author":"1","article_type":"original","date_created":"2024-01-21T23:00:57Z","ddc":["530"],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"volume":20,"related_material":{"link":[{"relation":"press_release","description":"News on ISTA Website","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/"}]},"has_accepted_license":"1","quality_controlled":"1","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"page":"310-321","intvolume":" 20","status":"public","language":[{"iso":"eng"}],"external_id":{"isi":["001138880800005"],"pmid":["38370025"]},"author":[{"last_name":"Caballero Mancebo","orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Shinde, Rushikesh","first_name":"Rushikesh","last_name":"Shinde"},{"first_name":"Madison","full_name":"Bolger-Munro, Madison","orcid":"0000-0002-8176-4824","last_name":"Bolger-Munro","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E"},{"full_name":"Peruzzo, Matilda","first_name":"Matilda","orcid":"0000-0002-3415-4628","last_name":"Peruzzo","id":"3F920B30-F248-11E8-B48F-1D18A9856A87"},{"id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory","full_name":"Szep, Gregory","last_name":"Szep"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","full_name":"Steccari, Irene","last_name":"Steccari"},{"last_name":"Labrousse Arias","first_name":"David","full_name":"Labrousse Arias, David","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425"},{"first_name":"Vanessa","full_name":"Zheden, Vanessa","orcid":"0000-0002-9438-4783","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Callan-Jones","full_name":"Callan-Jones, Andrew","first_name":"Andrew"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}]},{"page":"102-127","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"quality_controlled":"1","title":"Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM","language":[{"iso":"eng"}],"external_id":{"pmid":["38200359"]},"author":[{"first_name":"Ana","full_name":"Pimenta-Marques, Ana","last_name":"Pimenta-Marques"},{"last_name":"Perestrelo","full_name":"Perestrelo, Tania","first_name":"Tania"},{"id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","orcid":"0000-0003-1681-508X","last_name":"Dos Reis Rodrigues","full_name":"Dos Reis Rodrigues, Patricia","first_name":"Patricia"},{"first_name":"Paulo","full_name":"Duarte, Paulo","last_name":"Duarte"},{"last_name":"Ferreira-Silva","full_name":"Ferreira-Silva, Ana","first_name":"Ana"},{"last_name":"Lince-Faria","full_name":"Lince-Faria, Mariana","first_name":"Mariana"},{"last_name":"Bettencourt-Dias","full_name":"Bettencourt-Dias, Mónica","first_name":"Mónica"}],"status":"public","intvolume":" 25","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2024-02-04T23:00:53Z","ddc":["570"],"article_type":"original","has_accepted_license":"1","volume":25,"_id":"14933","day":"10","publication":"EMBO Reports","file_date_updated":"2024-02-05T12:35:03Z","issue":"1","type":"journal_article","citation":{"chicago":"Pimenta-Marques, Ana, Tania Perestrelo, Patricia Dos Reis Rodrigues, Paulo Duarte, Ana Ferreira-Silva, Mariana Lince-Faria, and Mónica Bettencourt-Dias. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” EMBO Reports. Embo Press, 2024. https://doi.org/10.1038/s44319-023-00020-6.","ieee":"A. Pimenta-Marques et al., “Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM,” EMBO Reports, vol. 25, no. 1. Embo Press, pp. 102–127, 2024.","ista":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, Duarte P, Ferreira-Silva A, Lince-Faria M, Bettencourt-Dias M. 2024. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. EMBO Reports. 25(1), 102–127.","apa":"Pimenta-Marques, A., Perestrelo, T., Dos Reis Rodrigues, P., Duarte, P., Ferreira-Silva, A., Lince-Faria, M., & Bettencourt-Dias, M. (2024). Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. EMBO Reports. Embo Press. https://doi.org/10.1038/s44319-023-00020-6","short":"A. Pimenta-Marques, T. Perestrelo, P. Dos Reis Rodrigues, P. Duarte, A. Ferreira-Silva, M. Lince-Faria, M. Bettencourt-Dias, EMBO Reports 25 (2024) 102–127.","ama":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, et al. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. EMBO Reports. 2024;25(1):102-127. doi:10.1038/s44319-023-00020-6","mla":"Pimenta-Marques, Ana, et al. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” EMBO Reports, vol. 25, no. 1, Embo Press, 2024, pp. 102–27, doi:10.1038/s44319-023-00020-6."},"department":[{"_id":"MiSi"}],"oa":1,"doi":"10.1038/s44319-023-00020-6","acknowledgement":"We thank all members of the Cell Cycle and Regulation Lab for the discussions and for the critical reading of the manuscript. We thank Tomer Avidor-Reiss (University of Toledo, Toledo, OH), Daniel St. Johnston (The Gurdon Institute, Cambridge, UK), David Glover (University of Cambridge, Cambridge, UK), Jingyan Fu (Agricultural University, Beijing, China) Jordan Raff (University of Oxford, Oxford, UK) and Timothy Megraw (Florida State University, Tallahassee, FL) for sharing tools. We acknowledge the technical support of Instituto Gulbenkian de Ciência (IGC)‘s Advanced Imaging Facility, in particular Gabriel Martins, Nuno Pimpão Martins and José Marques. We also thank Tiago Paixão from the IGC’s Quantitative & Digital Science Unit and Marco Louro from the CCR lab for the support provided on statistical analysis. IGC’s Advanced Imaging Facility (AIF-UIC) is supported by the national Portuguese funding ref# PPBI-POCI-01-0145-FEDER -022122. We thank the IGC’s Fly Facility, supported by CONGENTO (LISBOA-01-0145-FEDER-022170). This work was supported by an ERC grant (ERC-2015-CoG-683258) awarded to MBD and a grant from the Portuguese Research Council (FCT) awarded to APM (PTDC/BIA-BID/32225/2017).","publication_identifier":{"eissn":["1469-3178"]},"file":[{"content_type":"application/pdf","file_name":"2023_EmboReports_PimentaMarques.pdf","checksum":"53c3ef43d9bd6d7bff3ffcf57d763cac","file_size":9645056,"success":1,"date_updated":"2024-02-05T12:35:03Z","file_id":"14941","access_level":"open_access","creator":"dernst","date_created":"2024-02-05T12:35:03Z","relation":"main_file"}],"oa_version":"Published Version","article_processing_charge":"Yes (in subscription journal)","publication_status":"published","date_published":"2024-01-10T00:00:00Z","publisher":"Embo Press","date_updated":"2025-04-23T07:39:52Z","month":"01","abstract":[{"text":"Centrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, are downregulated in oogenesis and sufficient for maintaining both centrosome structural integrity and MTOC activity. We now show that the expression of specific components of the centriole cartwheel and wall, including ANA1/CEP295, is essential for maintaining centrosome integrity. We find that Polo kinase requires ANA1 to promote centriole stability in cultured cells and eggs. In addition, ANA1 expression prevents the loss of centrioles observed upon PCM-downregulation. However, the centrioles maintained by overexpressing and tethering ANA1 are inactive, unlike the MTOCs observed upon tethering Polo kinase. These findings demonstrate that several centriole components are needed to maintain centrosome structure. Our study also highlights that centrioles are more dynamic than previously believed, with their structural stability relying on the continuous expression of multiple components.","lang":"eng"}],"year":"2024","scopus_import":"1"},{"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix","quality_controlled":"1","external_id":{"pmid":["38506714"],"isi":["001264190100001"]},"author":[{"id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina","full_name":"Zens, Bettina","orcid":"0000-0002-9561-1239","last_name":"Zens"},{"full_name":"Fäßler, Florian","first_name":"Florian","orcid":"0000-0001-7149-769X","last_name":"Fäßler","id":"404F5528-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-7967-2085","last_name":"Hansen","first_name":"Jesse","full_name":"Hansen, Jesse","id":"1063c618-6f9b-11ec-9123-f912fccded63"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Datler","orcid":"0000-0002-3616-8580","full_name":"Datler, Julia","first_name":"Julia","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87"},{"id":"3661B498-F248-11E8-B48F-1D18A9856A87","full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","orcid":"0000-0003-3904-947X","last_name":"Hodirnau"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","full_name":"Zheden, Vanessa","orcid":"0000-0002-9438-4783","last_name":"Zheden"},{"id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","first_name":"Jonna H","full_name":"Alanko, Jonna H","orcid":"0000-0002-7698-3061","last_name":"Alanko"},{"full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","last_name":"Schur","orcid":"0000-0003-4790-8078","first_name":"Florian KM","full_name":"Schur, Florian KM"}],"language":[{"iso":"eng"}],"intvolume":" 223","status":"public","article_number":"e202309125","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"article_type":"original","corr_author":"1","ddc":["570"],"date_created":"2024-03-21T06:45:51Z","has_accepted_license":"1","volume":223,"ec_funded":1,"day":"20","_id":"15146","issue":"6","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"M-Shop"}],"file_date_updated":"2024-03-25T12:52:04Z","publication":"Journal of Cell Biology","oa":1,"citation":{"ista":"Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V, Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6), e202309125.","apa":"Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V., … Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.202309125","short":"B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V. Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).","mla":"Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” Journal of Cell Biology, vol. 223, no. 6, e202309125, Rockefeller University Press, 2024, doi:10.1083/jcb.202309125.","ama":"Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 2024;223(6). doi:10.1083/jcb.202309125","chicago":"Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” Journal of Cell Biology. Rockefeller University Press, 2024. https://doi.org/10.1083/jcb.202309125.","ieee":"B. Zens et al., “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix,” Journal of Cell Biology, vol. 223, no. 6. Rockefeller University Press, 2024."},"type":"journal_article","department":[{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"acknowledgement":"Open Access funding provided by IST Austria. We thank Armel Nicolas and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante, and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the Miba Machine shop for their support. We also thank Wanda Kukulski (University of Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group for helpful discussions. Matt Swulius and Jessica Heebner provided great support in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure illustration.\r\n\r\nThis research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Lab Support Facility, and the Electron Microscopy Facility. We acknowledge funding support from the following sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds (to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt), and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to F.K.M. Schur).","doi":"10.1083/jcb.202309125","file":[{"file_id":"15188","access_level":"open_access","creator":"dernst","date_created":"2024-03-25T12:52:04Z","relation":"main_file","content_type":"application/pdf","file_name":"2024_JCB_Zens.pdf","checksum":"90d1984a93660735e506c2a304bc3f73","file_size":11907016,"success":1,"date_updated":"2024-03-25T12:52:04Z"}],"oa_version":"Published Version","publication_status":"published","project":[{"grant_number":"P33367","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex"},{"_id":"7bd318a1-9f16-11ee-852c-cc9217763180","name":"In Situ Actin Structures via Hybrid Cryo-electron Microscopy","grant_number":"E435"},{"call_identifier":"H2020","grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÃ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"},{"name":"Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis","_id":"2615199A-B435-11E9-9278-68D0E5697425","grant_number":"21317"},{"grant_number":"CZI01","_id":"62909c6f-2b32-11ec-9570-e1476aab5308","name":"CryoMinflux-guided in-situ visual proteomics and structure determination"}],"article_processing_charge":"Yes (via OA deal)","publisher":"Rockefeller University Press","date_published":"2024-03-20T00:00:00Z","scopus_import":"1","abstract":[{"lang":"eng","text":"The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly."}],"year":"2024","isi":1,"month":"03","date_updated":"2025-09-04T13:17:16Z"},{"status":"public","intvolume":" 154","language":[{"iso":"eng"}],"author":[{"full_name":"Link, Kristina","first_name":"Kristina","last_name":"Link"},{"full_name":"Muhandes, Lina","first_name":"Lina","last_name":"Muhandes"},{"full_name":"Polikarpova, Anastasia","first_name":"Anastasia","last_name":"Polikarpova"},{"full_name":"Lämmermann, Tim","first_name":"Tim","last_name":"Lämmermann"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Reinhard","full_name":"Fässler, Reinhard","last_name":"Fässler"},{"full_name":"Roers, Axel","first_name":"Axel","last_name":"Roers"}],"external_id":{"isi":["001308886700001"],"pmid":["38636606"]},"quality_controlled":"1","title":"Integrin β1–mediated mast cell immune-surveillance of blood vessel content","page":"745-753","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"volume":154,"OA_type":"hybrid","has_accepted_license":"1","date_created":"2024-05-19T22:01:13Z","ddc":["570"],"article_type":"original","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1016/j.jaci.2024.03.022","acknowledgement":"This work was funded by Deutsche Forschungsgemeinschaft, Germany, grants RO2133/ 9-1 and RO2133/ 9-2 in the setting of FOR2599 and TR156 project C11 (Project-ID 246807620–TRR 156) to A. Roers and Springboard-to-Postdoc grant of the Dresden International Graduate School for Biomedicine and Bioengineering (DIGS-BB), Dresden, Germany, and Fond zur Förderung der Wissenschaftlichen Forschung (FWF), Austria, Hertha Firnberg grant (project number T-1219) to A. Polikarpova.\r\nWe thank Dr Michael Gerlach, Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, TU Dresden, for expert support of in vivo imaging experiments; Grace Wurigamule for help with 2-photon imaging and flow cytometric analysis of mouse skin; and Christina Hiller, Livia Schulze, Madelaine Rickauer, and Christa Haase for providing expert technical assistance.","publication_identifier":{"issn":["0091-6749"],"eissn":["1097-6825"]},"department":[{"_id":"MiSi"}],"citation":{"chicago":"Link, Kristina, Lina Muhandes, Anastasia Polikarpova, Tim Lämmermann, Michael K Sixt, Reinhard Fässler, and Axel Roers. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” Journal of Allergy and Clinical Immunology. Elsevier, 2024. https://doi.org/10.1016/j.jaci.2024.03.022.","ieee":"K. Link et al., “Integrin β1–mediated mast cell immune-surveillance of blood vessel content,” Journal of Allergy and Clinical Immunology, vol. 154, no. 3. Elsevier, pp. 745–753, 2024.","ista":"Link K, Muhandes L, Polikarpova A, Lämmermann T, Sixt MK, Fässler R, Roers A. 2024. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. Journal of Allergy and Clinical Immunology. 154(3), 745–753.","apa":"Link, K., Muhandes, L., Polikarpova, A., Lämmermann, T., Sixt, M. K., Fässler, R., & Roers, A. (2024). Integrin β1–mediated mast cell immune-surveillance of blood vessel content. Journal of Allergy and Clinical Immunology. Elsevier. https://doi.org/10.1016/j.jaci.2024.03.022","short":"K. Link, L. Muhandes, A. Polikarpova, T. Lämmermann, M.K. Sixt, R. Fässler, A. Roers, Journal of Allergy and Clinical Immunology 154 (2024) 745–753.","mla":"Link, Kristina, et al. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” Journal of Allergy and Clinical Immunology, vol. 154, no. 3, Elsevier, 2024, pp. 745–53, doi:10.1016/j.jaci.2024.03.022.","ama":"Link K, Muhandes L, Polikarpova A, et al. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. Journal of Allergy and Clinical Immunology. 2024;154(3):745-753. doi:10.1016/j.jaci.2024.03.022"},"type":"journal_article","oa":1,"publication":"Journal of Allergy and Clinical Immunology","file_date_updated":"2025-01-13T10:55:28Z","issue":"3","day":"01","_id":"15408","date_updated":"2025-09-08T07:28:25Z","isi":1,"month":"09","abstract":[{"text":"Background: IgE-mediated degranulation of mast cells (MCs) provides rapid protection against environmental hazards, including animal venoms. A fraction of tissue-resident MCs intimately associates with blood vessels. These perivascular MCs were reported to extend projections into the vessel lumen and to be the first MCs to acquire intravenously injected IgE, suggesting that IgE loading of MCs depends on their vascular association.\r\nObjective: We sought to elucidate the molecular basis of the MC–blood vessel interaction and to determine its relevance for IgE-mediated immune responses.\r\nMethods: We selectively inactivated the Itgb1 gene, encoding the β1 chain of integrin adhesion molecules (ITGB1), in MCs by conditional gene targeting in mice. We analyzed skin MCs for blood vessel association, surface IgE density, and capability to bind circulating antibody specific for MC surface molecules, as well as in vivo responses to antigen administered via different routes.\r\nResults: Lack of ITGB1 expression severely compromised MC–blood vessel association. ITGB1-deficient MCs showed normal densities of surface IgE but reduced binding of intravenously injected antibodies. While their capacity to degranulate in response to IgE ligation in vivo was unimpaired, anaphylactic responses to antigen circulating in the vasculature were largely abolished.\r\nConclusions: ITGB1-mediated association of MCs with blood vessels is key for MC immune surveillance of blood vessel content, but is dispensable for slow steady-state loading of endogenous IgE onto tissue-resident MCs.","lang":"eng"}],"scopus_import":"1","year":"2024","date_published":"2024-09-01T00:00:00Z","publisher":"Elsevier","article_processing_charge":"Yes (in subscription journal)","OA_place":"publisher","publication_status":"published","file":[{"file_size":1792425,"success":1,"date_updated":"2025-01-13T10:55:28Z","content_type":"application/pdf","file_name":"2024_JourAllergyClinicalImm_Link.pdf","checksum":"6a5af05082e1869d7cad6406fa4eb76c","access_level":"open_access","creator":"dernst","date_created":"2025-01-13T10:55:28Z","relation":"main_file","file_id":"18840"}],"oa_version":"Published Version"},{"publisher":"Springer Nature","date_published":"2024-06-21T00:00:00Z","isi":1,"month":"06","date_updated":"2025-09-08T08:06:56Z","abstract":[{"lang":"eng","text":"Dendritic cells migrate to and from lymph nodes in response to chemokine gradients.Data now show that steady-state migration of these cells can be triggered by a mechanosensitive pathway."}],"scopus_import":"1","year":"2024","oa_version":"None","article_processing_charge":"No","publication_status":"published","department":[{"_id":"MiSi"}],"type":"journal_article","citation":{"chicago":"Lembo, Sergio, and Michael K Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” Nature Immunology. Springer Nature, 2024. https://doi.org/10.1038/s41590-024-01881-2.","ieee":"S. Lembo and M. K. Sixt, “Nuclear squeezing wakes up dendritic cells,” Nature Immunology, vol. 25. Springer Nature, pp. 1131–1132, 2024.","apa":"Lembo, S., & Sixt, M. K. (2024). Nuclear squeezing wakes up dendritic cells. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-024-01881-2","ista":"Lembo S, Sixt MK. 2024. Nuclear squeezing wakes up dendritic cells. Nature Immunology. 25, 1131–1132.","ama":"Lembo S, Sixt MK. Nuclear squeezing wakes up dendritic cells. Nature Immunology. 2024;25:1131–1132. doi:10.1038/s41590-024-01881-2","mla":"Lembo, Sergio, and Michael K. Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” Nature Immunology, vol. 25, Springer Nature, 2024, pp. 1131–1132, doi:10.1038/s41590-024-01881-2.","short":"S. Lembo, M.K. Sixt, Nature Immunology 25 (2024) 1131–1132."},"publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"doi":"10.1038/s41590-024-01881-2","_id":"17191","day":"21","publication":"Nature Immunology","volume":25,"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-06-30T22:01:05Z","article_type":"letter_note","corr_author":"1","external_id":{"pmid":["38907047"],"isi":["001251509300001"]},"author":[{"id":"d993a7b2-292f-11ed-aaac-fb045a912e31","orcid":"0000-0002-2253-8771","last_name":"Lembo","first_name":"Sergio","full_name":"Lembo, Sergio"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"status":"public","intvolume":" 25","page":"1131–1132 ","title":"Nuclear squeezing wakes up dendritic cells","quality_controlled":"1"},{"volume":14,"has_accepted_license":"1","date_created":"2024-07-14T22:01:11Z","ddc":["570"],"article_type":"original","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"e5029","status":"public","intvolume":" 14","language":[{"iso":"eng"}],"external_id":{"pmid":["39007160"]},"author":[{"id":"922e68bb-1727-11ee-857c-966e8cc1b6c3","full_name":"Li, Ziqiang","first_name":"Ziqiang","last_name":"Li"},{"last_name":"Huard","first_name":"Jennifer","full_name":"Huard, Jennifer"},{"last_name":"Bayer","first_name":"Emmanuelle M.","full_name":"Bayer, Emmanuelle M."},{"first_name":"Valérie","full_name":"Wattelet-Boyer, Valérie","last_name":"Wattelet-Boyer"}],"quality_controlled":"1","title":"Versatile cloning strategy for efficient multigene editing in Arabidopsis","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2025-03-06T10:28:18Z","month":"07","year":"2024","scopus_import":"1","abstract":[{"lang":"eng","text":"CRISPR-Cas9 technology has become an essential tool for plant genome editing. Recent advancements have significantly improved the ability to target multiple genes simultaneously within the same genetic background through various strategies. Additionally, there has been significant progress in developing methods for inducible or tissue-specific editing. These advancements offer numerous possibilities for tailored genome modifications. Building upon existing research, we have developed an optimized and modular strategy allowing the targeting of several genes simultaneously in combination with the synchronized expression of the Cas9 endonuclease in the egg cell. This system allows significant editing efficiency while avoiding mosaicism. In addition, the versatile system we propose allows adaptation to inducible and/or tissue-specific edition according to the promoter chosen to drive the expression of the Cas9 gene. Here, we describe a step-by-step protocol for generating the binary vector necessary for establishing Arabidopsis edited lines using a versatile cloning strategy that combines Gateway® and Golden Gate technologies. We describe a versatile system that allows the cloning of as many guides as needed to target DNA, which can be multiplexed into a polycistronic gene and combined in the same construct with sequences for the expression of the Cas9 endonuclease. The expression of Cas9 is controlled by selecting from among a collection of promoters, including constitutive, inducible, ubiquitous, or tissue-specific promoters. Only one vector containing the polycistronic gene (tRNA-sgRNA) needs to be constructed. For that, sgRNA (composed of protospacers chosen to target the gene of interest and sgRNA scaffold) is cloned in tandem with the pre-tRNA sequence. Then, a single recombination reaction is required to assemble the promoter, the zCas9 coding sequence, and the tRNA-gRNA polycistronic gene. Each element is cloned in an entry vector and finally assembled according to the Multisite Gateway® Technology. Here, we detail the process to express zCas9 under the control of egg cell promoter fused to enhancer sequence (EC1.2en-EC1.1p) and to simultaneously target two multiple C2 domains and transmembrane region protein genes (MCTP3 and MCTP4, respectively at3g57880 and at1g51570), using one or two sgRNA per gene."}],"date_published":"2024-07-05T00:00:00Z","publisher":"Bio-Protocol","article_processing_charge":"Yes","publication_status":"published","file":[{"file_id":"17242","date_created":"2024-07-16T06:16:11Z","relation":"main_file","access_level":"open_access","creator":"dernst","file_name":"2024_BioProtocol_Li.pdf","checksum":"c8671c0ad483da6407cb16cc3fef1990","content_type":"application/pdf","success":1,"date_updated":"2024-07-16T06:16:11Z","file_size":2896048}],"oa_version":"Published Version","doi":"10.21769/BioProtoc.5029","acknowledgement":"This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (project 772103-BRIDGING to E.M.B.).","publication_identifier":{"eissn":["2331-8325"]},"citation":{"ama":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. Versatile cloning strategy for efficient multigene editing in Arabidopsis. Bio-protocol. 2024;14(13). doi:10.21769/BioProtoc.5029","mla":"LI, ZIQIANG, et al. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” Bio-Protocol, vol. 14, no. 13, e5029, Bio-Protocol, 2024, doi:10.21769/BioProtoc.5029.","short":"Z. LI, J. Huard, E.M. Bayer, V. Wattelet-Boyer, Bio-Protocol 14 (2024).","ista":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. 2024. Versatile cloning strategy for efficient multigene editing in Arabidopsis. Bio-protocol. 14(13), e5029.","apa":"LI, Z., Huard, J., Bayer, E. M., & Wattelet-Boyer, V. (2024). Versatile cloning strategy for efficient multigene editing in Arabidopsis. Bio-Protocol. Bio-Protocol. https://doi.org/10.21769/BioProtoc.5029","ieee":"Z. LI, J. Huard, E. M. Bayer, and V. Wattelet-Boyer, “Versatile cloning strategy for efficient multigene editing in Arabidopsis,” Bio-protocol, vol. 14, no. 13. Bio-Protocol, 2024.","chicago":"LI, ZIQIANG, Jennifer Huard, Emmanuelle M. Bayer, and Valérie Wattelet-Boyer. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” Bio-Protocol. Bio-Protocol, 2024. https://doi.org/10.21769/BioProtoc.5029."},"department":[{"_id":"MiSi"}],"type":"journal_article","oa":1,"publication":"Bio-protocol","file_date_updated":"2024-07-16T06:16:11Z","issue":"13","day":"05","_id":"17233"},{"page":"1242-1243","quality_controlled":"1","title":"Rescuing T cells from stiff tumors","language":[{"iso":"eng"}],"author":[{"last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X","full_name":"Avellaneda Sarrió, Mario","first_name":"Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K"}],"external_id":{"isi":["001275725000001"],"pmid":["39029454"]},"status":"public","intvolume":" 31","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-07-21T22:01:00Z","corr_author":"1","article_type":"review","volume":31,"day":"18","_id":"17279","publication":"Cell Chemical Biology","issue":"7","type":"journal_article","department":[{"_id":"MiSi"}],"citation":{"chicago":"Avellaneda Sarrió, Mario, and Michael K Sixt. “Rescuing T Cells from Stiff Tumors.” Cell Chemical Biology. Elsevier, 2024. https://doi.org/10.1016/j.chembiol.2024.06.011.","ieee":"M. Avellaneda Sarrió and M. K. Sixt, “Rescuing T cells from stiff tumors,” Cell Chemical Biology, vol. 31, no. 7. Elsevier, pp. 1242–1243, 2024.","apa":"Avellaneda Sarrió, M., & Sixt, M. K. (2024). Rescuing T cells from stiff tumors. Cell Chemical Biology. Elsevier. https://doi.org/10.1016/j.chembiol.2024.06.011","ista":"Avellaneda Sarrió M, Sixt MK. 2024. Rescuing T cells from stiff tumors. Cell Chemical Biology. 31(7), 1242–1243.","ama":"Avellaneda Sarrió M, Sixt MK. Rescuing T cells from stiff tumors. Cell Chemical Biology. 2024;31(7):1242-1243. doi:10.1016/j.chembiol.2024.06.011","mla":"Avellaneda Sarrió, Mario, and Michael K. Sixt. “Rescuing T Cells from Stiff Tumors.” Cell Chemical Biology, vol. 31, no. 7, Elsevier, 2024, pp. 1242–43, doi:10.1016/j.chembiol.2024.06.011.","short":"M. Avellaneda Sarrió, M.K. Sixt, Cell Chemical Biology 31 (2024) 1242–1243."},"doi":"10.1016/j.chembiol.2024.06.011","publication_identifier":{"issn":["2451-9456"],"eissn":["2451-9448"]},"oa_version":"None","article_processing_charge":"No","publication_status":"published","date_published":"2024-07-18T00:00:00Z","publisher":"Elsevier","date_updated":"2025-09-08T08:27:03Z","month":"07","isi":1,"abstract":[{"text":"In a recent issue of Cell, Zhang et al.1 demonstrate that mechanical features of a solid tumor can drive T cells into dysfunctionality and identify pathways that revert this “exhausted” state.","lang":"eng"}],"scopus_import":"1","year":"2024"},{"quality_controlled":"1","title":"Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis","page":"645-653","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"status":"public","intvolume":" 631","language":[{"iso":"eng"}],"external_id":{"isi":["001281636500020"],"pmid":["38987596"]},"author":[{"orcid":"0000-0001-6120-3723","last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ishikawa-Ankerhold","full_name":"Ishikawa-Ankerhold, Hellen","first_name":"Hellen"},{"full_name":"Stutte, Susanne","first_name":"Susanne","last_name":"Stutte"},{"full_name":"Fu, Wenwen","first_name":"Wenwen","last_name":"Fu"},{"last_name":"Weitz","first_name":"Jutta","full_name":"Weitz, Jutta"},{"last_name":"Dueck","full_name":"Dueck, Anne","first_name":"Anne"},{"full_name":"Nelakuditi, Bhavishya","first_name":"Bhavishya","last_name":"Nelakuditi"},{"first_name":"Valeria","full_name":"Fumagalli, Valeria","last_name":"Fumagalli"},{"last_name":"Van Den Heuvel","first_name":"Dominic","full_name":"Van Den Heuvel, Dominic"},{"first_name":"Larissa","full_name":"Belz, Larissa","last_name":"Belz"},{"last_name":"Sobirova","first_name":"Gulnoza","full_name":"Sobirova, Gulnoza"},{"last_name":"Zhang","full_name":"Zhang, Zhe","first_name":"Zhe"},{"full_name":"Titova, Anna","first_name":"Anna","last_name":"Titova"},{"first_name":"Alejandro Martinez","full_name":"Navarro, Alejandro Martinez","last_name":"Navarro"},{"last_name":"Pekayvaz","first_name":"Kami","full_name":"Pekayvaz, Kami"},{"last_name":"Lorenz","first_name":"Michael","full_name":"Lorenz, Michael"},{"last_name":"Von Baumgarten","first_name":"Louisa","full_name":"Von Baumgarten, Louisa"},{"last_name":"Kranich","first_name":"Jan","full_name":"Kranich, Jan"},{"full_name":"Straub, Tobias","first_name":"Tobias","last_name":"Straub"},{"first_name":"Bastian","full_name":"Popper, Bastian","last_name":"Popper"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa","first_name":"Vanessa"},{"orcid":"0000-0001-9735-5315","last_name":"Kaufmann","first_name":"Walter","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Guo","first_name":"Chenglong","full_name":"Guo, Chenglong"},{"first_name":"Guido","full_name":"Piontek, Guido","last_name":"Piontek"},{"first_name":"Saskia","full_name":"Von Stillfried, Saskia","last_name":"Von Stillfried"},{"full_name":"Boor, Peter","first_name":"Peter","last_name":"Boor"},{"full_name":"Colonna, Marco","first_name":"Marco","last_name":"Colonna"},{"full_name":"Clauß, Sebastian","first_name":"Sebastian","last_name":"Clauß"},{"first_name":"Christian","full_name":"Schulz, Christian","last_name":"Schulz"},{"last_name":"Brocker","full_name":"Brocker, Thomas","first_name":"Thomas"},{"full_name":"Walzog, Barbara","first_name":"Barbara","last_name":"Walzog"},{"full_name":"Scheiermann, Christoph","first_name":"Christoph","last_name":"Scheiermann"},{"last_name":"Aird","first_name":"William C.","full_name":"Aird, William C."},{"last_name":"Nerlov","full_name":"Nerlov, Claus","first_name":"Claus"},{"last_name":"Stark","full_name":"Stark, Konstantin","first_name":"Konstantin"},{"last_name":"Petzold","first_name":"Tobias","full_name":"Petzold, Tobias"},{"full_name":"Engelhardt, Stefan","first_name":"Stefan","last_name":"Engelhardt"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild"},{"last_name":"Rudelius","full_name":"Rudelius, Martina","first_name":"Martina"},{"last_name":"Oostendorp","first_name":"Robert A.J.","full_name":"Oostendorp, Robert A.J."},{"last_name":"Iannacone","full_name":"Iannacone, Matteo","first_name":"Matteo"},{"last_name":"Heinig","full_name":"Heinig, Matthias","first_name":"Matthias"},{"last_name":"Massberg","first_name":"Steffen","full_name":"Massberg, Steffen"}],"date_created":"2024-07-21T22:01:02Z","ddc":["570"],"corr_author":"1","article_type":"original","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","ec_funded":1,"volume":631,"related_material":{"link":[{"url":"https://github.com/heiniglab/gaertner_megakaryocytes","relation":"software"}]},"has_accepted_license":"1","publication":"Nature","file_date_updated":"2024-07-22T06:16:11Z","day":"18","_id":"17284","doi":"10.1038/s41586-024-07671-y","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"acknowledgement":"We thank S. Helmer, N. Blount, E. Raatz and Z. Sisic for technical assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) SFB 1123 (S.M. project B06); SFB 914 (S.M. projects B02 and Z01, H.I.-A. project Z01, S.S. project A06, K.S. project B02, C. Schulz project A10, B.W. project A02, C. Scheiermann project B09); SFB 1054 (T.B. project B03); FOR2033 (F.G., R.A.J.O., S.M.); Individual research grant project ID: 514478744 (F.G.); Heisenberg Programme project ID: 514477451 (F.G.); the DZHK (German Center for Cardiovascular Research) (MHA 1.4VD (S.M.), Postdoc Start-up Grant, 81×3600213 (F.G.)); and LMUexcellence NFF (F.G.). W.F. received funding from China Scholarship Council (CSC, no. 201306270012). P.B. is supported by the German Research Foundation (DFG, project IDs 322900939, 432698239 and 445703531), European Research Council (ERC Consolidator grant no. 101001791) and the Federal Ministry of Education and Research (BMBF, STOP-FSGS-01GM2202C and NATON within the framework of the Network of University Medicine, no. 01KX2121). S.v.S. is supported by the START-Program of the Faculty of Medicine of the RWTH Aachen University (AZ 125/17). A.D. and S.E. are supported by the German Research Foundation (SFB TRR 267); S.E. by the BMBF in the framework of the Cluster4future program (CNATM—Cluster for Nucleic Acid Therapeutics Munich). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 833440 to S.M.). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687. The project is funded by the European Union (ERC, MEKanics, 101078110). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.","department":[{"_id":"EM-Fac"},{"_id":"MiSi"},{"_id":"Bio"}],"citation":{"ieee":"F. R. Gärtner et al., “Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis,” Nature, vol. 631. Springer Nature, pp. 645–653, 2024.","chicago":"Gärtner, Florian R, Hellen Ishikawa-Ankerhold, Susanne Stutte, Wenwen Fu, Jutta Weitz, Anne Dueck, Bhavishya Nelakuditi, et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” Nature. Springer Nature, 2024. https://doi.org/10.1038/s41586-024-07671-y.","short":"F.R. Gärtner, H. Ishikawa-Ankerhold, S. Stutte, W. Fu, J. Weitz, A. Dueck, B. Nelakuditi, V. Fumagalli, D. Van Den Heuvel, L. Belz, G. Sobirova, Z. Zhang, A. Titova, A.M. Navarro, K. Pekayvaz, M. Lorenz, L. Von Baumgarten, J. Kranich, T. Straub, B. Popper, V. Zheden, W. Kaufmann, C. Guo, G. Piontek, S. Von Stillfried, P. Boor, M. Colonna, S. Clauß, C. Schulz, T. Brocker, B. Walzog, C. Scheiermann, W.C. Aird, C. Nerlov, K. Stark, T. Petzold, S. Engelhardt, M.K. Sixt, R. Hauschild, M. Rudelius, R.A.J. Oostendorp, M. Iannacone, M. Heinig, S. Massberg, Nature 631 (2024) 645–653.","mla":"Gärtner, Florian R., et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” Nature, vol. 631, Springer Nature, 2024, pp. 645–53, doi:10.1038/s41586-024-07671-y.","ama":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. 2024;631:645-653. doi:10.1038/s41586-024-07671-y","ista":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, Fu W, Weitz J, Dueck A, Nelakuditi B, Fumagalli V, Van Den Heuvel D, Belz L, Sobirova G, Zhang Z, Titova A, Navarro AM, Pekayvaz K, Lorenz M, Von Baumgarten L, Kranich J, Straub T, Popper B, Zheden V, Kaufmann W, Guo C, Piontek G, Von Stillfried S, Boor P, Colonna M, Clauß S, Schulz C, Brocker T, Walzog B, Scheiermann C, Aird WC, Nerlov C, Stark K, Petzold T, Engelhardt S, Sixt MK, Hauschild R, Rudelius M, Oostendorp RAJ, Iannacone M, Heinig M, Massberg S. 2024. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. 631, 645–653.","apa":"Gärtner, F. R., Ishikawa-Ankerhold, H., Stutte, S., Fu, W., Weitz, J., Dueck, A., … Massberg, S. (2024). Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. Springer Nature. https://doi.org/10.1038/s41586-024-07671-y"},"type":"journal_article","oa":1,"article_processing_charge":"Yes (in subscription journal)","project":[{"call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"publication_status":"published","file":[{"file_id":"17286","access_level":"open_access","creator":"dernst","date_created":"2024-07-22T06:16:11Z","relation":"main_file","content_type":"application/pdf","file_name":"2024_Nature_Gaertner.pdf","checksum":"aa004afc72d2489f0fb0fcbc9919fbbd","file_size":15704819,"date_updated":"2024-07-22T06:16:11Z","success":1}],"oa_version":"Published Version","date_updated":"2025-09-08T08:14:25Z","isi":1,"month":"07","abstract":[{"text":"Platelet homeostasis is essential for vascular integrity and immune defence1,2. Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, the cellular and molecular mechanisms required to constantly replenish the pool of MKs by their progenitor cells (megakaryopoiesis) remains unclear3,4. Here we use intravital imaging to track the cellular dynamics of megakaryopoiesis over days. We identify plasmacytoid dendritic cells (pDCs) as homeostatic sensors that monitor the bone marrow for apoptotic MKs and deliver IFNα to the MK niche triggering local on-demand proliferation and maturation of MK progenitors. This pDC-dependent feedback loop is crucial for MK and platelet homeostasis at steady state and under stress. pDCs are best known for their ability to function as vigilant detectors of viral infection5. We show that virus-induced activation of pDCs interferes with their function as homeostatic sensors of megakaryopoiesis. Consequently, activation of pDCs by SARS-CoV-2 leads to excessive megakaryopoiesis. Together, we identify a pDC-dependent homeostatic circuit that involves innate immune sensing and demand-adapted release of inflammatory mediators to maintain homeostasis of the megakaryocytic lineage.","lang":"eng"}],"scopus_import":"1","year":"2024","date_published":"2024-07-18T00:00:00Z","publisher":"Springer Nature"},{"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"Synchronization in collectively moving inanimate and living active matter","quality_controlled":"1","external_id":{"pmid":["37704595"],"isi":["001087583700030"]},"author":[{"last_name":"Riedl","orcid":"0000-0003-4844-6311","first_name":"Michael","full_name":"Riedl, Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"},{"id":"61763940-15b2-11ec-abd3-cfaddfbc66b4","last_name":"Mayer","full_name":"Mayer, Isabelle D","first_name":"Isabelle D"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K"},{"id":"3A374330-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2057-2754","last_name":"Hof","first_name":"Björn","full_name":"Hof, Björn"}],"language":[{"iso":"eng"}],"intvolume":" 14","status":"public","article_number":"5633","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"article_type":"original","corr_author":"1","ddc":["530","570"],"date_created":"2023-09-24T22:01:10Z","has_accepted_license":"1","volume":14,"ec_funded":1,"day":"13","_id":"14361","file_date_updated":"2023-09-25T08:32:37Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"publication":"Nature Communications","oa":1,"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"BjHo"}],"type":"journal_article","citation":{"ista":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. 2023. Synchronization in collectively moving inanimate and living active matter. Nature Communications. 14, 5633.","apa":"Riedl, M., Mayer, I. D., Merrin, J., Sixt, M. K., & Hof, B. (2023). Synchronization in collectively moving inanimate and living active matter. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-023-41432-1","short":"M. Riedl, I.D. Mayer, J. Merrin, M.K. Sixt, B. Hof, Nature Communications 14 (2023).","mla":"Riedl, Michael, et al. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” Nature Communications, vol. 14, 5633, Springer Nature, 2023, doi:10.1038/s41467-023-41432-1.","ama":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. Synchronization in collectively moving inanimate and living active matter. Nature Communications. 2023;14. doi:10.1038/s41467-023-41432-1","chicago":"Riedl, Michael, Isabelle D Mayer, Jack Merrin, Michael K Sixt, and Björn Hof. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” Nature Communications. Springer Nature, 2023. https://doi.org/10.1038/s41467-023-41432-1.","ieee":"M. Riedl, I. D. Mayer, J. Merrin, M. K. Sixt, and B. Hof, “Synchronization in collectively moving inanimate and living active matter,” Nature Communications, vol. 14. Springer Nature, 2023."},"publication_identifier":{"eissn":["2041-1723"]},"acknowledgement":"We thank K. O’Keeffe, E. Hannezo, P. Devreotes, C. Dessalles, and E. Martens for discussion and/or critical reading of the manuscript; the Bioimaging Facility of ISTA for excellent support, as well as the Life Science Facility and the Miba Machine Shop of ISTA. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S.","doi":"10.1038/s41467-023-41432-1","file":[{"content_type":"application/pdf","file_name":"2023_NatureComm_Riedl.pdf","checksum":"82d2d4ad736cc8493db8ce45cd313f7b","file_size":2317272,"success":1,"date_updated":"2023-09-25T08:32:37Z","file_id":"14366","access_level":"open_access","creator":"dernst","date_created":"2023-09-25T08:32:37Z","relation":"main_file"}],"oa_version":"Published Version","publication_status":"published","project":[{"grant_number":"281556","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"Yes","publisher":"Springer Nature","date_published":"2023-09-13T00:00:00Z","scopus_import":"1","abstract":[{"text":"Whether one considers swarming insects, flocking birds, or bacterial colonies, collective motion arises from the coordination of individuals and entails the adjustment of their respective velocities. In particular, in close confinements, such as those encountered by dense cell populations during development or regeneration, collective migration can only arise coordinately. Yet, how individuals unify their velocities is often not understood. Focusing on a finite number of cells in circular confinements, we identify waves of polymerizing actin that function as a pacemaker governing the speed of individual cells. We show that the onset of collective motion coincides with the synchronization of the wave nucleation frequencies across the population. Employing a simpler and more readily accessible mechanical model system of active spheres, we identify the synchronization of the individuals’ internal oscillators as one of the essential requirements to reach the corresponding collective state. The mechanical ‘toy’ experiment illustrates that the global synchronous state is achieved by nearest neighbor coupling. We suggest by analogy that local coupling and the synchronization of actin waves are essential for the emergent, self-organized motion of cell collectives.","lang":"eng"}],"year":"2023","isi":1,"month":"09","date_updated":"2025-04-14T13:10:03Z"},{"title":"The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction","quality_controlled":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"status":"public","intvolume":" 11","external_id":{"isi":["001100762800001"],"pmid":["38020899"]},"author":[{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","last_name":"Riedl","orcid":"0000-0003-4844-6311","first_name":"Michael","full_name":"Riedl, Michael"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"ddc":["570"],"date_created":"2023-11-19T23:00:55Z","article_type":"original","corr_author":"1","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"1287420","volume":11,"has_accepted_license":"1","publication":"Frontiers in Cell and Developmental Biology","file_date_updated":"2023-11-20T08:41:15Z","_id":"14555","day":"31","publication_identifier":{"eissn":["2296-634X"]},"acknowledgement":"The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.","doi":"10.3389/fcell.2023.1287420","type":"journal_article","department":[{"_id":"MiSi"}],"citation":{"ieee":"M. Riedl and M. K. Sixt, “The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction,” Frontiers in Cell and Developmental Biology, vol. 11. Frontiers, 2023.","chicago":"Riedl, Michael, and Michael K Sixt. “The Excitable Nature of Polymerizing Actin and the Belousov-Zhabotinsky Reaction.” Frontiers in Cell and Developmental Biology. Frontiers, 2023. https://doi.org/10.3389/fcell.2023.1287420.","ama":"Riedl M, Sixt MK. The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. Frontiers in Cell and Developmental Biology. 2023;11. doi:10.3389/fcell.2023.1287420","mla":"Riedl, Michael, and Michael K. Sixt. “The Excitable Nature of Polymerizing Actin and the Belousov-Zhabotinsky Reaction.” Frontiers in Cell and Developmental Biology, vol. 11, 1287420, Frontiers, 2023, doi:10.3389/fcell.2023.1287420.","short":"M. Riedl, M.K. Sixt, Frontiers in Cell and Developmental Biology 11 (2023).","apa":"Riedl, M., & Sixt, M. K. (2023). The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. Frontiers in Cell and Developmental Biology. Frontiers. https://doi.org/10.3389/fcell.2023.1287420","ista":"Riedl M, Sixt MK. 2023. The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. Frontiers in Cell and Developmental Biology. 11, 1287420."},"oa":1,"article_processing_charge":"Yes","publication_status":"published","oa_version":"Published Version","file":[{"file_id":"14561","date_created":"2023-11-20T08:41:15Z","relation":"main_file","access_level":"open_access","creator":"dernst","file_name":"2023_FrontiersCellDevBio_Riedl.pdf","checksum":"61857fc3ebf019354932e7ee684658ce","content_type":"application/pdf","success":1,"date_updated":"2023-11-20T08:41:15Z","file_size":2047622}],"isi":1,"month":"10","date_updated":"2025-09-09T13:22:00Z","year":"2023","scopus_import":"1","abstract":[{"text":"The intricate regulatory processes behind actin polymerization play a crucial role in cellular biology, including essential mechanisms such as cell migration or cell division. However, the self-organizing principles governing actin polymerization are still poorly understood. In this perspective article, we compare the Belousov-Zhabotinsky (BZ) reaction, a classic and well understood chemical oscillator known for its self-organizing spatiotemporal dynamics, with the excitable dynamics of polymerizing actin. While the BZ reaction originates from the domain of inorganic chemistry, it shares remarkable similarities with actin polymerization, including the characteristic propagating waves, which are influenced by geometry and external fields, and the emergent collective behavior. Starting with a general description of emerging patterns, we elaborate on single droplets or cell-level dynamics, the influence of geometric confinements and conclude with collective interactions. Comparing these two systems sheds light on the universal nature of self-organization principles in both living and inanimate systems.","lang":"eng"}],"publisher":"Frontiers","date_published":"2023-10-31T00:00:00Z"},{"page":"137-147","title":"En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses","quality_controlled":"1","external_id":{"pmid":["37106180"]},"author":[{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F","full_name":"Leithner, Alexander F","last_name":"Leithner","orcid":"0000-0002-1073-744X"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","full_name":"Merrin, Jack"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"language":[{"iso":"eng"}],"intvolume":" 2654","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"date_created":"2023-05-22T08:41:48Z","volume":2654,"ec_funded":1,"place":"New York, NY","_id":"13052","day":"28","editor":[{"last_name":"Baldari","first_name":"Cosima","full_name":"Baldari, Cosima"},{"full_name":"Dustin, Michael","first_name":"Michael","last_name":"Dustin"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"},{"_id":"M-Shop"}],"publication":"The Immune Synapse","type":"book_chapter","department":[{"_id":"MiSi"},{"_id":"NanoFab"}],"citation":{"chicago":"Leithner, Alexander F, Jack Merrin, and Michael K Sixt. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” In The Immune Synapse, edited by Cosima Baldari and Michael Dustin, 2654:137–47. MIMB. New York, NY: Springer Nature, 2023. https://doi.org/10.1007/978-1-0716-3135-5_9.","ieee":"A. F. Leithner, J. Merrin, and M. K. Sixt, “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses,” in The Immune Synapse, vol. 2654, C. Baldari and M. Dustin, Eds. New York, NY: Springer Nature, 2023, pp. 137–147.","apa":"Leithner, A. F., Merrin, J., & Sixt, M. K. (2023). En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In C. Baldari & M. Dustin (Eds.), The Immune Synapse (Vol. 2654, pp. 137–147). New York, NY: Springer Nature. https://doi.org/10.1007/978-1-0716-3135-5_9","ista":"Leithner AF, Merrin J, Sixt MK. 2023.En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: The Immune Synapse. Methods in Molecular Biology, vol. 2654, 137–147.","short":"A.F. Leithner, J. Merrin, M.K. Sixt, in:, C. Baldari, M. Dustin (Eds.), The Immune Synapse, Springer Nature, New York, NY, 2023, pp. 137–147.","ama":"Leithner AF, Merrin J, Sixt MK. En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: Baldari C, Dustin M, eds. The Immune Synapse. Vol 2654. MIMB. New York, NY: Springer Nature; 2023:137-147. doi:10.1007/978-1-0716-3135-5_9","mla":"Leithner, Alexander F., et al. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” The Immune Synapse, edited by Cosima Baldari and Michael Dustin, vol. 2654, Springer Nature, 2023, pp. 137–47, doi:10.1007/978-1-0716-3135-5_9."},"publication_identifier":{"eissn":["1940-6029"],"eisbn":["9781071631355"],"issn":["1064-3745"],"isbn":["9781071631348"]},"acknowledgement":"A.L. was funded by an Erwin Schrödinger postdoctoral fellowship of the Austrian Science Fund (FWF, project number: J4542-B) and is an EMBO non-stipendiary postdoctoral fellow. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. We thank the Imaging & Optics facility, the Nanofabrication facility, and the Miba Machine Shop of ISTA for their excellent support.","doi":"10.1007/978-1-0716-3135-5_9","oa_version":"None","publication_status":"published","article_processing_charge":"No","project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","call_identifier":"H2020","grant_number":"724373"}],"series_title":"MIMB","publisher":"Springer Nature","date_published":"2023-04-28T00:00:00Z","year":"2023","abstract":[{"lang":"eng","text":"Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell contacts along the vertical imaging plane. This requires optical sectioning that often results in unsatisfactory resolution in time and space. Here, we present a workflow where DCs and T cells are confined between a layer of glass and polydimethylsiloxane (PDMS) that orients the cells along one, horizontal imaging plane, allowing for fast en-face-imaging of the DC-T cell IS."}],"scopus_import":"1","alternative_title":["Methods in Molecular Biology"],"month":"04","date_updated":"2025-04-14T07:42:07Z"},{"quality_controlled":"1","publication":"Biophysics of Molecular Chaperones","title":"Probing Single Chaperone Substrates","page":"278-318","editor":[{"first_name":"Sebastian","full_name":"Hiller, Sebastian","last_name":"Hiller"},{"first_name":"Maili","full_name":"Liu, Maili","last_name":"Liu"},{"full_name":"He, Lichun","first_name":"Lichun","last_name":"He"}],"day":"01","_id":"14848","status":"public","intvolume":" 29","doi":"10.1039/bk9781839165986-00278","publication_identifier":{"isbn":["9781839162824"],"eisbn":["9781839165993"]},"citation":{"apa":"Wruck, F., Avellaneda Sarrió, M., Naqvi, M. M., Koers, E. J., Till, K., Gross, L., … Tans, S. J. (2023). Probing Single Chaperone Substrates. In S. Hiller, M. Liu, & L. He (Eds.), Biophysics of Molecular Chaperones (Vol. 29, pp. 278–318). Royal Society of Chemistry. https://doi.org/10.1039/bk9781839165986-00278","ista":"Wruck F, Avellaneda Sarrió M, Naqvi MM, Koers EJ, Till K, Gross L, Moayed F, Roland A, Heling LWHJ, Mashaghi A, Tans SJ. 2023.Probing Single Chaperone Substrates. In: Biophysics of Molecular Chaperones. New Developments in NMR, vol. 29, 278–318.","short":"F. Wruck, M. Avellaneda Sarrió, M.M. Naqvi, E.J. Koers, K. Till, L. Gross, F. Moayed, A. Roland, L.W.H.J. Heling, A. Mashaghi, S.J. Tans, in:, S. Hiller, M. Liu, L. He (Eds.), Biophysics of Molecular Chaperones, Royal Society of Chemistry, 2023, pp. 278–318.","ama":"Wruck F, Avellaneda Sarrió M, Naqvi MM, et al. Probing Single Chaperone Substrates. In: Hiller S, Liu M, He L, eds. Biophysics of Molecular Chaperones. Vol 29. Royal Society of Chemistry; 2023:278-318. doi:10.1039/bk9781839165986-00278","mla":"Wruck, F., et al. “Probing Single Chaperone Substrates.” Biophysics of Molecular Chaperones, edited by Sebastian Hiller et al., vol. 29, Royal Society of Chemistry, 2023, pp. 278–318, doi:10.1039/bk9781839165986-00278.","chicago":"Wruck, F., Mario Avellaneda Sarrió, M. M. Naqvi, E. J. Koers, K. Till, L. Gross, F. Moayed, et al. “Probing Single Chaperone Substrates.” In Biophysics of Molecular Chaperones, edited by Sebastian Hiller, Maili Liu, and Lichun He, 29:278–318. Royal Society of Chemistry, 2023. https://doi.org/10.1039/bk9781839165986-00278.","ieee":"F. Wruck et al., “Probing Single Chaperone Substrates,” in Biophysics of Molecular Chaperones, vol. 29, S. Hiller, M. Liu, and L. He, Eds. Royal Society of Chemistry, 2023, pp. 278–318."},"department":[{"_id":"MiSi"}],"type":"book_chapter","language":[{"iso":"eng"}],"author":[{"first_name":"F.","full_name":"Wruck, F.","last_name":"Wruck"},{"orcid":"0000-0001-6406-524X","last_name":"Avellaneda Sarrió","full_name":"Avellaneda Sarrió, Mario","first_name":"Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"first_name":"M. M.","full_name":"Naqvi, M. M.","last_name":"Naqvi"},{"last_name":"Koers","first_name":"E. J.","full_name":"Koers, E. J."},{"full_name":"Till, K.","first_name":"K.","last_name":"Till"},{"first_name":"L.","full_name":"Gross, L.","last_name":"Gross"},{"full_name":"Moayed, F.","first_name":"F.","last_name":"Moayed"},{"last_name":"Roland","first_name":"A.","full_name":"Roland, A."},{"full_name":"Heling, L. W. H. J.","first_name":"L. W. H. J.","last_name":"Heling"},{"last_name":"Mashaghi","full_name":"Mashaghi, A.","first_name":"A."},{"last_name":"Tans","first_name":"S. J.","full_name":"Tans, S. J."}],"article_processing_charge":"No","date_created":"2024-01-22T08:07:02Z","publication_status":"published","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-01-23T12:01:53Z","month":"11","volume":29,"alternative_title":["New Developments in NMR"],"abstract":[{"text":"Regulating protein states is considered the core function of chaperones. However, despite their importance to all major cellular processes, the conformational changes that chaperones impart on polypeptide chains are difficult to study directly due to their heterogeneous, dynamic, and multi-step nature. Here, we review recent advances towards this aim using single-molecule manipulation methods, which are rapidly revealing new mechanisms of conformational control and helping to define a different perspective on the chaperone function.","lang":"eng"}],"year":"2023","date_published":"2023-11-01T00:00:00Z","publisher":"Royal Society of Chemistry"},{"has_accepted_license":"1","related_material":{"record":[{"id":"461","relation":"part_of_dissertation","status":"public"},{"id":"10791","status":"public","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","status":"public","id":"7932"},{"status":"public","relation":"part_of_dissertation","id":"10703"},{"status":"public","relation":"old_edition","id":"12726"}]},"keyword":["Synchronization","Collective Movement","Active Matter","Cell Migration","Active Colloids"],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ddc":["530","570"],"date_created":"2023-11-15T09:59:03Z","corr_author":"1","author":[{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","first_name":"Michael","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","last_name":"Riedl"}],"language":[{"iso":"eng"}],"status":"public","supervisor":[{"orcid":"0000-0003-2057-2754","last_name":"Hof","full_name":"Hof, Björn","first_name":"Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87"}],"page":"260","title":"Synchronization in collectively moving active matter","publisher":"Institute of Science and Technology Austria","date_published":"2023-11-16T00:00:00Z","month":"11","date_updated":"2026-04-07T13:29:13Z","abstract":[{"lang":"eng","text":"Most motions of many-body systems at any scale in nature with sufficient degrees of freedom tend to be chaotic; reaching from the orbital motion of planets, the air currents in our atmosphere, down to the water flowing through our pipelines or the movement of a population of bacteria. To the observer it is therefore intriguing when a moving collective exhibits order. Collective motion of flocks of birds, schools of fish or swarms of self-propelled particles or robots have been studied extensively over the past decades but the mechanisms involved in the transition from chaos to order remain unclear. Here, the interactions, that in most systems give rise to chaos, sustain order. In this thesis we investigate mechanisms that preserve, destabilize or lead to the ordered state. We show that endothelial cells migrating in circular confinements transition to a collective rotating state and concomitantly synchronize the frequencies of nucleating actin waves within individual cells. Consequently, the frequency dependent cell migration speed uniformizes across the population. Complementary to the WAVE dependent nucleation of traveling actin waves, we show that in leukocytes the actin polymerization depending on WASp generates pushing forces locally at stationary patches. Next, in pipe flows, we study methods to disrupt the self--sustaining cycle of turbulence and therefore relaminarize the flow. While we find in pulsating flow conditions that turbulence emerges through a helical instability during the decelerating phase. Finally, we show quantitatively in brain slices of mice that wild-type control neurons can compensate the migratory deficits of a genetically modified neuronal sub--population in the developing cortex. "}],"year":"2023","alternative_title":["ISTA Thesis"],"file":[{"content_type":"application/pdf","checksum":"52e1d0ab6c1abe59c82dfe8c9ff5f83a","file_name":"Thesis_Riedl_2023_corr.pdf","file_size":36743942,"success":1,"date_updated":"2023-11-15T09:52:54Z","file_id":"14536","creator":"mriedl","access_level":"open_access","relation":"main_file","date_created":"2023-11-15T09:52:54Z"}],"oa_version":"Updated Version","article_processing_charge":"No","publication_status":"published","OA_place":"publisher","citation":{"ieee":"M. Riedl, “Synchronization in collectively moving active matter,” Institute of Science and Technology Austria, 2023.","chicago":"Riedl, Michael. “Synchronization in Collectively Moving Active Matter.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/14530.","short":"M. Riedl, Synchronization in Collectively Moving Active Matter, Institute of Science and Technology Austria, 2023.","mla":"Riedl, Michael. Synchronization in Collectively Moving Active Matter. Institute of Science and Technology Austria, 2023, doi:10.15479/14530.","ama":"Riedl M. Synchronization in collectively moving active matter. 2023. doi:10.15479/14530","apa":"Riedl, M. (2023). Synchronization in collectively moving active matter. Institute of Science and Technology Austria. https://doi.org/10.15479/14530","ista":"Riedl M. 2023. Synchronization in collectively moving active matter. Institute of Science and Technology Austria."},"type":"dissertation","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"oa":1,"degree_awarded":"PhD","publication_identifier":{"issn":["2663-337X"]},"doi":"10.15479/14530","day":"16","_id":"14530","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"file_date_updated":"2023-11-15T09:52:54Z"}]