---
_id: '2215'
abstract:
- lang: eng
text: Homologous recombination is crucial for genome stability and for genetic exchange.
Although our knowledge of the principle steps in recombination and its machinery
is well advanced, homology search, the critical step of exploring the genome for
homologous sequences to enable recombination, has remained mostly enigmatic. However,
recent methodological advances have provided considerable new insights into this
fundamental step in recombination that can be integrated into a mechanistic model.
These advances emphasize the importance of genomic proximity and nuclear organization
for homology search and the critical role of homology search mediators in this
process. They also aid our understanding of how homology search might lead to
unwanted and potentially disease-promoting recombination events.
acknowledgement: J.R. was supported by a Boehringer Ingelheim Fonds PhD stipend.
article_processing_charge: No
author:
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
- first_name: Claudio
full_name: Lademann, Claudio
last_name: Lademann
- first_name: Stefan
full_name: Jentsch, Stefan
last_name: Jentsch
citation:
ama: Renkawitz J, Lademann C, Jentsch S. Mechanisms and principles of homology search
during recombination. Nature Reviews Molecular Cell Biology. 2014;15(6):369-383.
doi:10.1038/nrm3805
apa: Renkawitz, J., Lademann, C., & Jentsch, S. (2014). Mechanisms and principles
of homology search during recombination. Nature Reviews Molecular Cell Biology.
Nature Publishing Group. https://doi.org/10.1038/nrm3805
chicago: Renkawitz, Jörg, Claudio Lademann, and Stefan Jentsch. “Mechanisms and
Principles of Homology Search during Recombination.” Nature Reviews Molecular
Cell Biology. Nature Publishing Group, 2014. https://doi.org/10.1038/nrm3805.
ieee: J. Renkawitz, C. Lademann, and S. Jentsch, “Mechanisms and principles of homology
search during recombination,” Nature Reviews Molecular Cell Biology, vol.
15, no. 6. Nature Publishing Group, pp. 369–383, 2014.
ista: Renkawitz J, Lademann C, Jentsch S. 2014. Mechanisms and principles of homology
search during recombination. Nature Reviews Molecular Cell Biology. 15(6), 369–383.
mla: Renkawitz, Jörg, et al. “Mechanisms and Principles of Homology Search during
Recombination.” Nature Reviews Molecular Cell Biology, vol. 15, no. 6,
Nature Publishing Group, 2014, pp. 369–83, doi:10.1038/nrm3805.
short: J. Renkawitz, C. Lademann, S. Jentsch, Nature Reviews Molecular Cell Biology
15 (2014) 369–383.
date_created: 2018-12-11T11:56:22Z
date_published: 2014-05-14T00:00:00Z
date_updated: 2025-09-29T11:30:10Z
day: '14'
department:
- _id: MiSi
doi: 10.1038/nrm3805
external_id:
isi:
- '000337245500009'
intvolume: ' 15'
isi: 1
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 369 - 383
publication: Nature Reviews Molecular Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '4755'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanisms and principles of homology search during recombination
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2014'
...
---
_id: '2242'
abstract:
- lang: eng
text: MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in
many cellular pathways. MiRNAs associate with members of the Argonaute protein
family and bind to partially complementary sequences on mRNAs and induce translational
repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized
miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and
macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct
influence of miR-155 on the expression levels of other miRNAs. For example, miR-455
is negatively regulated in miR-155-deficient cells possibly due to inhibition
of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data
sets, we propose a model of hierarchical miRNA expression dominated by miR-155
in DCs and macrophages.
article_processing_charge: No
author:
- first_name: Anne
full_name: Dueck, Anne
last_name: Dueck
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Gunter
full_name: Meister, Gunter
last_name: Meister
citation:
ama: Dueck A, Eichner A, Sixt MK, Meister G. A miR-155-dependent microRNA hierarchy
in dendritic cell maturation and macrophage activation. FEBS Letters. 2014;588(4):632-640.
doi:10.1016/j.febslet.2014.01.009
apa: Dueck, A., Eichner, A., Sixt, M. K., & Meister, G. (2014). A miR-155-dependent
microRNA hierarchy in dendritic cell maturation and macrophage activation. FEBS
Letters. Elsevier. https://doi.org/10.1016/j.febslet.2014.01.009
chicago: Dueck, Anne, Alexander Eichner, Michael K Sixt, and Gunter Meister. “A
MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell Maturation and Macrophage
Activation.” FEBS Letters. Elsevier, 2014. https://doi.org/10.1016/j.febslet.2014.01.009.
ieee: A. Dueck, A. Eichner, M. K. Sixt, and G. Meister, “A miR-155-dependent microRNA
hierarchy in dendritic cell maturation and macrophage activation,” FEBS Letters,
vol. 588, no. 4. Elsevier, pp. 632–640, 2014.
ista: Dueck A, Eichner A, Sixt MK, Meister G. 2014. A miR-155-dependent microRNA
hierarchy in dendritic cell maturation and macrophage activation. FEBS Letters.
588(4), 632–640.
mla: Dueck, Anne, et al. “A MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell
Maturation and Macrophage Activation.” FEBS Letters, vol. 588, no. 4, Elsevier,
2014, pp. 632–40, doi:10.1016/j.febslet.2014.01.009.
short: A. Dueck, A. Eichner, M.K. Sixt, G. Meister, FEBS Letters 588 (2014) 632–640.
date_created: 2018-12-11T11:56:31Z
date_published: 2014-02-14T00:00:00Z
date_updated: 2025-09-29T11:19:23Z
day: '14'
department:
- _id: MiSi
doi: 10.1016/j.febslet.2014.01.009
external_id:
isi:
- '000331115700016'
intvolume: ' 588'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 632 - 640
publication: FEBS Letters
publication_identifier:
issn:
- 0014-5793
publication_status: published
publisher: Elsevier
publist_id: '4714'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage
activation
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 588
year: '2014'
...
---
_id: '2830'
article_processing_charge: No
author:
- first_name: Christine
full_name: Moussion, Christine
id: 3356F664-F248-11E8-B48F-1D18A9856A87
last_name: Moussion
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Moussion C, Sixt MK. A conduit to amplify innate immunity. Immunity.
2013;38(5):853-854. doi:10.1016/j.immuni.2013.05.005
apa: Moussion, C., & Sixt, M. K. (2013). A conduit to amplify innate immunity.
Immunity. Cell Press. https://doi.org/10.1016/j.immuni.2013.05.005
chicago: Moussion, Christine, and Michael K Sixt. “A Conduit to Amplify Innate Immunity.”
Immunity. Cell Press, 2013. https://doi.org/10.1016/j.immuni.2013.05.005.
ieee: C. Moussion and M. K. Sixt, “A conduit to amplify innate immunity,” Immunity,
vol. 38, no. 5. Cell Press, pp. 853–854, 2013.
ista: Moussion C, Sixt MK. 2013. A conduit to amplify innate immunity. Immunity.
38(5), 853–854.
mla: Moussion, Christine, and Michael K. Sixt. “A Conduit to Amplify Innate Immunity.”
Immunity, vol. 38, no. 5, Cell Press, 2013, pp. 853–54, doi:10.1016/j.immuni.2013.05.005.
short: C. Moussion, M.K. Sixt, Immunity 38 (2013) 853–854.
corr_author: '1'
date_created: 2018-12-11T11:59:49Z
date_published: 2013-05-23T00:00:00Z
date_updated: 2025-09-29T13:51:00Z
day: '23'
department:
- _id: MiSi
doi: 10.1016/j.immuni.2013.05.005
external_id:
isi:
- '000330942500005'
intvolume: ' 38'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa_version: None
page: 853 - 854
publication: Immunity
publication_status: published
publisher: Cell Press
publist_id: '3969'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A conduit to amplify innate immunity
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 38
year: '2013'
...
---
_id: '2839'
abstract:
- lang: eng
text: Directional guidance of cells via gradients of chemokines is considered crucial
for embryonic development, cancer dissemination, and immune responses. Nevertheless,
the concept still lacks direct experimental confirmation in vivo. Here, we identify
endogenous gradients of the chemokine CCL21 within mouse skin and show that they
guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots
of CCL21 within lymphatic endothelial cells and steeply decaying gradients within
the perilymphatic interstitium. These gradients match the migratory patterns of
the dendritic cells, which directionally approach vessels from a distance of up
to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and
its experimental delocalization or swamping the endogenous gradients abolishes
directed migration. These findings functionally establish the concept of haptotaxis,
directed migration along immobilized gradients, in tissues.
acknowledgement: We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst,
and E. Kiermaier for critical reading of the manuscript. This work was supported
by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research
Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011
to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss
National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).
article_processing_charge: No
article_type: original
author:
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Jan
full_name: Schwarz, Jan
id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Christine
full_name: Moussion, Christine
id: 3356F664-F248-11E8-B48F-1D18A9856A87
last_name: Moussion
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Daniel
full_name: Legler, Daniel
last_name: Legler
- first_name: Sanjiv
full_name: Luther, Sanjiv
last_name: Luther
- first_name: Mark Tobias
full_name: Bollenbach, Mark Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
orcid: 0000-0003-4398-476X
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance
by haptotactic chemokine gradients. Science. 2013;339(6117):328-332. doi:10.1126/science.1228456
apa: Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler,
D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic
chemokine gradients. Science. American Association for the Advancement
of Science. https://doi.org/10.1126/science.1228456
chicago: Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid
de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K
Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.”
Science. American Association for the Advancement of Science, 2013. https://doi.org/10.1126/science.1228456.
ieee: M. Weber et al., “Interstitial dendritic cell guidance by haptotactic
chemokine gradients,” Science, vol. 339, no. 6117. American Association
for the Advancement of Science, pp. 328–332, 2013.
ista: Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther
S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic
chemokine gradients. Science. 339(6117), 328–332.
mla: Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic
Chemokine Gradients.” Science, vol. 339, no. 6117, American Association
for the Advancement of Science, 2013, pp. 328–32, doi:10.1126/science.1228456.
short: M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler,
S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.
corr_author: '1'
date_created: 2018-12-11T11:59:52Z
date_published: 2013-01-18T00:00:00Z
date_updated: 2025-09-29T13:45:52Z
day: '18'
department:
- _id: MiSi
- _id: Bio
doi: 10.1126/science.1228456
ec_funded: 1
external_id:
isi:
- '000313622000047'
intvolume: ' 339'
isi: 1
issue: '6117'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf
month: '01'
oa: 1
oa_version: Published Version
page: 328 - 332
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25ABD200-B435-11E9-9278-68D0E5697425
grant_number: RGP0058/2011
name: 'Cell migration in complex environments: from in vivo experiments to theoretical
models'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3959'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Interstitial dendritic cell guidance by haptotactic chemokine gradients
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 339
year: '2013'
...
---
_id: '10900'
abstract:
- lang: eng
text: Leukocyte migration through the interstitial space is crucial for the maintenance
of tolerance and immunity. The main cues for leukocyte trafficking are chemokines
thought to directionally guide these cells towards their targets. However, model
systems that facilitate quantification of chemokine-guided leukocyte migration
in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted
mouse ears that allows the visualization of chemokine-dependent dendritic cell
(DC) motility in the dermal interstitium in real time. We present methods for
the preparation of mouse ear sheets and their use in multidimensional confocal
imaging experiments to monitor and analyze the directional migration of fluorescently
labelled DCs through the dermis and into afferent lymphatic vessels. The assay
provides a more physiological approach to study leukocyte migration than in vitro
three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen
gels and transwell assays.
acknowledgement: We would like to thank Alexander Eichner and Ingrid de Vries for
discussion and critical reading of the manuscript, and Mary Frank for assistance
with the recording of videos and images in Fig. 1. M.S. is supported through funding
from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt
Foundation for funding.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration
in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. Chemokines.
Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:10.1007/978-1-62703-426-5_14'
apa: 'Weber, M., & Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic
Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona & E. Ubogu
(Eds.), Chemokines (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press.
https://doi.org/10.1007/978-1-62703-426-5_14'
chicago: 'Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic
Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In Chemokines,
edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ:
Humana Press, 2013. https://doi.org/10.1007/978-1-62703-426-5_14.'
ieee: 'M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell
Migration in Explanted Mouse Ear Preparations,” in Chemokines, vol. 1013,
A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.'
ista: 'Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration
in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology,
vol. 1013, 215–226.'
mla: Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic
Cell Migration in Explanted Mouse Ear Preparations.” Chemokines, edited
by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26,
doi:10.1007/978-1-62703-426-5_14.
short: M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana
Press, Totowa, NJ, 2013, pp. 215–226.
corr_author: '1'
date_created: 2022-03-21T07:47:41Z
date_published: 2013-04-03T00:00:00Z
date_updated: 2024-10-09T21:02:37Z
day: '03'
department:
- _id: MiSi
doi: 10.1007/978-1-62703-426-5_14
editor:
- first_name: Astrid
full_name: Cardona, Astrid
last_name: Cardona
- first_name: Eroboghene
full_name: Ubogu, Eroboghene
last_name: Ubogu
external_id:
pmid:
- '23625502'
intvolume: ' 1013'
language:
- iso: eng
month: '04'
oa_version: None
page: 215-226
place: Totowa, NJ
pmid: 1
publication: Chemokines
publication_identifier:
eisbn:
- '9781627034265'
eissn:
- 1940-6029
isbn:
- '9781627034258'
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse
Ear Preparations
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 1013
year: '2013'
...
---
_id: '522'
abstract:
- lang: eng
text: Podoplanin, a mucin-like plasma membrane protein, is expressed by lymphatic
endothelial cells and responsible for separation of blood and lymphatic circulation
through activation of platelets. Here we show that podoplanin is also expressed
by thymic fibroblastic reticular cells (tFRC), a novel thymic medulla stroma cell
type associated with thymic conduits, and involved in development of natural regulatory
T cells (nTreg). Young mice deficient in podoplanin lack nTreg owing to retardation
of CD4+CD25+ thymocytes in the cortex and missing differentiation of Foxp3+ thymocytes
in the medulla. This might be due to CCL21 that delocalizes upon deletion of the
CCL21-binding podoplanin from medullar tFRC to cortex areas. The animals do not
remain devoid of nTreg but generate them delayed within the first month resulting
in Th2-biased hypergammaglobulinemia but not in the death-causing autoimmune phenotype
of Foxp3-deficient Scurfy mice.
article_processing_charge: No
author:
- first_name: Elke
full_name: Fuertbauer, Elke
last_name: Fuertbauer
- first_name: Jan
full_name: Zaujec, Jan
last_name: Zaujec
- first_name: Pavel
full_name: Uhrin, Pavel
last_name: Uhrin
- first_name: Ingrid
full_name: Raab, Ingrid
last_name: Raab
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
- first_name: Helga
full_name: Schachner, Helga
last_name: Schachner
- first_name: Miroslav
full_name: Bauer, Miroslav
last_name: Bauer
- first_name: Gerhard
full_name: Schütz, Gerhard
last_name: Schütz
- first_name: Bernd
full_name: Binder, Bernd
last_name: Binder
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Dontscho
full_name: Kerjaschki, Dontscho
last_name: Kerjaschki
- first_name: Hannes
full_name: Stockinger, Hannes
last_name: Stockinger
citation:
ama: Fuertbauer E, Zaujec J, Uhrin P, et al. Thymic medullar conduits-associated
podoplanin promotes natural regulatory T cells. Immunology Letters. 2013;154(1-2):31-41.
doi:10.1016/j.imlet.2013.07.007
apa: Fuertbauer, E., Zaujec, J., Uhrin, P., Raab, I., Weber, M., Schachner, H.,
… Stockinger, H. (2013). Thymic medullar conduits-associated podoplanin promotes
natural regulatory T cells. Immunology Letters. Elsevier. https://doi.org/10.1016/j.imlet.2013.07.007
chicago: Fuertbauer, Elke, Jan Zaujec, Pavel Uhrin, Ingrid Raab, Michele Weber,
Helga Schachner, Miroslav Bauer, et al. “Thymic Medullar Conduits-Associated Podoplanin
Promotes Natural Regulatory T Cells.” Immunology Letters. Elsevier, 2013.
https://doi.org/10.1016/j.imlet.2013.07.007.
ieee: E. Fuertbauer et al., “Thymic medullar conduits-associated podoplanin
promotes natural regulatory T cells,” Immunology Letters, vol. 154, no.
1–2. Elsevier, pp. 31–41, 2013.
ista: Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, Bauer M, Schütz
G, Binder B, Sixt MK, Kerjaschki D, Stockinger H. 2013. Thymic medullar conduits-associated
podoplanin promotes natural regulatory T cells. Immunology Letters. 154(1–2),
31–41.
mla: Fuertbauer, Elke, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes
Natural Regulatory T Cells.” Immunology Letters, vol. 154, no. 1–2, Elsevier,
2013, pp. 31–41, doi:10.1016/j.imlet.2013.07.007.
short: E. Fuertbauer, J. Zaujec, P. Uhrin, I. Raab, M. Weber, H. Schachner, M. Bauer,
G. Schütz, B. Binder, M.K. Sixt, D. Kerjaschki, H. Stockinger, Immunology Letters
154 (2013) 31–41.
date_created: 2018-12-11T11:46:57Z
date_published: 2013-07-01T00:00:00Z
date_updated: 2025-09-30T07:11:09Z
day: '01'
department:
- _id: MiSi
doi: 10.1016/j.imlet.2013.07.007
external_id:
isi:
- '000326559400006'
intvolume: ' 154'
isi: 1
issue: 1-2
language:
- iso: eng
month: '07'
oa_version: None
page: 31 - 41
publication: Immunology Letters
publication_status: published
publisher: Elsevier
publist_id: '7300'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Thymic medullar conduits-associated podoplanin promotes natural regulatory
T cells
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 154
year: '2013'
...
---
_id: '2945'
abstract:
- lang: eng
text: In search of foreign antigens, lymphocytes recirculate from the blood, through
lymph nodes, into lymphatics and back to the blood. Dendritic cells also migrate
to lymph nodes for optimal interaction with lymphocytes. This continuous trafficking
of immune cells into and out of lymph nodes is essential for immune surveillance
of foreign invaders. In this article, we review our current understanding of the
functions of high endothelial venules (HEVs), stroma and lymphatics in the entry,
positioning and exit of immune cells in lymph nodes during homeostasis, and we
highlight the unexpected role of dendritic cells in the control of lymphocyte
homing through HEVs.
acknowledgement: We thank M. Sixt and A. Peixoto for helpful comments on the manuscript.
Work in the laboratory of J.-P.G. is supported by grants from Fondation ARC pour
la Recherche sur le Cancer, Agence Nationale de la Recherche (ANR), Institut National
du Cancer (INCA), Fondation RITC and Région Midi-Pyrénées. Research by R.F. is supported
by Deutsche Forschungsgemeinschaft (DFG) grants SFB621-A1, SFB738-B5, SFB587-B3,
SFB900-B1 and KFO 250-FO 334/2-1. We regret that, owing to space limitations, we
could not always quote the work of colleagues who have contributed to the field.
article_processing_charge: No
author:
- first_name: Jean
full_name: Girard, Jean
last_name: Girard
- first_name: Christine
full_name: Moussion, Christine
id: 3356F664-F248-11E8-B48F-1D18A9856A87
last_name: Moussion
- first_name: Reinhold
full_name: Förster, Reinhold
last_name: Förster
citation:
ama: Girard J, Moussion C, Förster R. HEVs, lymphatics and homeostatic immune cell
trafficking in lymph nodes. Nature Reviews Immunology. 2012;12(11):762-773.
doi:10.1038/nri3298
apa: Girard, J., Moussion, C., & Förster, R. (2012). HEVs, lymphatics and homeostatic
immune cell trafficking in lymph nodes. Nature Reviews Immunology. Nature
Publishing Group. https://doi.org/10.1038/nri3298
chicago: Girard, Jean, Christine Moussion, and Reinhold Förster. “HEVs, Lymphatics
and Homeostatic Immune Cell Trafficking in Lymph Nodes.” Nature Reviews Immunology.
Nature Publishing Group, 2012. https://doi.org/10.1038/nri3298.
ieee: J. Girard, C. Moussion, and R. Förster, “HEVs, lymphatics and homeostatic
immune cell trafficking in lymph nodes,” Nature Reviews Immunology, vol.
12, no. 11. Nature Publishing Group, pp. 762–773, 2012.
ista: Girard J, Moussion C, Förster R. 2012. HEVs, lymphatics and homeostatic immune
cell trafficking in lymph nodes. Nature Reviews Immunology. 12(11), 762–773.
mla: Girard, Jean, et al. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking
in Lymph Nodes.” Nature Reviews Immunology, vol. 12, no. 11, Nature Publishing
Group, 2012, pp. 762–73, doi:10.1038/nri3298.
short: J. Girard, C. Moussion, R. Förster, Nature Reviews Immunology 12 (2012) 762–773.
date_created: 2018-12-11T12:00:29Z
date_published: 2012-11-01T00:00:00Z
date_updated: 2025-09-30T08:12:46Z
day: '01'
department:
- _id: MiSi
doi: 10.1038/nri3298
external_id:
isi:
- '000310523400010'
intvolume: ' 12'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa_version: None
page: 762 - 773
publication: Nature Reviews Immunology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3787'
quality_controlled: '1'
scopus_import: '1'
status: public
title: HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 12
year: '2012'
...
---
_id: '2946'
abstract:
- lang: eng
text: MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all
cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them
to specific target sites located in the 3′-untranslated region (3′-UTR) of target
mRNAs leading to translational repression and deadenylation-induced mRNA degradation.
Most miRNAs are processed from hairpin-structured precursors by the consecutive
action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451
is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we
have characterized miR-451 sequence and structure requirements for processing
as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears
to be optimized for Ago2 cleavage and changes result in reduced processing. In
addition, we show that the mature miR-451 only associates with Ago2 suggesting
that mature miRNAs are not exchanged between different members of the Ago protein
family. Based on cloning and deep sequencing of endogenous miRNAs associated with
Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago
identity appears to influence the length of some miRNAs, while others remain unaffected.
acknowledgement: "Deutsche Forschungsgemeinschaft (DFG) (SFB 960 and FOR855); European
Research Council (ERC grant ‘sRNAs’); European Union (FP7 project ‘ONCOMIRs’); German
Bundesministerium für Bildung und Forschung (BMBF, NGFN+, FKZ PIM-01GS0804-5); Bavarian
Genome Research Network (BayGene to G.M.); The Netherlands Organization for Scientific
Research (NWO, VIDI grant to E.B.). Funding for open access charge: DFG via the
open access publishing program. \r\n\r\nWe thank Sigrun Ammon and Corinna Friederich
for technical assistance and Sebastian Petri and Daniel Schraivogel for helpful
discussions."
article_processing_charge: No
author:
- first_name: Anne
full_name: Dueck, Anne
last_name: Dueck
- first_name: Christian
full_name: Ziegler, Christian
last_name: Ziegler
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Eugène
full_name: Berezikov, Eugène
last_name: Berezikov
- first_name: Gunter
full_name: Meister, Gunter
last_name: Meister
citation:
ama: Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. MicroRNAs associated
with the different human Argonaute proteins. Nucleic Acids Research. 2012;40(19):9850-9862.
doi:10.1093/nar/gks705
apa: Dueck, A., Ziegler, C., Eichner, A., Berezikov, E., & Meister, G. (2012).
MicroRNAs associated with the different human Argonaute proteins. Nucleic Acids
Research. Oxford University Press. https://doi.org/10.1093/nar/gks705
chicago: Dueck, Anne, Christian Ziegler, Alexander Eichner, Eugène Berezikov, and
Gunter Meister. “MicroRNAs Associated with the Different Human Argonaute Proteins.”
Nucleic Acids Research. Oxford University Press, 2012. https://doi.org/10.1093/nar/gks705.
ieee: A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, and G. Meister, “MicroRNAs
associated with the different human Argonaute proteins,” Nucleic Acids Research,
vol. 40, no. 19. Oxford University Press, pp. 9850–9862, 2012.
ista: Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. 2012. MicroRNAs associated
with the different human Argonaute proteins. Nucleic Acids Research. 40(19), 9850–9862.
mla: Dueck, Anne, et al. “MicroRNAs Associated with the Different Human Argonaute
Proteins.” Nucleic Acids Research, vol. 40, no. 19, Oxford University Press,
2012, pp. 9850–62, doi:10.1093/nar/gks705.
short: A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, G. Meister, Nucleic Acids
Research 40 (2012) 9850–9862.
corr_author: '1'
date_created: 2018-12-11T12:00:29Z
date_published: 2012-10-01T00:00:00Z
date_updated: 2025-09-30T08:12:07Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1093/nar/gks705
external_id:
isi:
- '000310377200046'
file:
- access_level: open_access
checksum: 1bb8d1ff894014b481657a21083c941c
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:12Z
date_updated: 2020-07-14T12:45:55Z
file_id: '4993'
file_name: IST-2015-383-v1+1_Nucl._Acids_Res.-2012-Dueck-9850-62.pdf
file_size: 8126936
relation: main_file
file_date_updated: 2020-07-14T12:45:55Z
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '19'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '10'
oa: 1
oa_version: Published Version
page: 9850 - 9862
publication: Nucleic Acids Research
publication_status: published
publisher: Oxford University Press
publist_id: '3786'
pubrep_id: '383'
quality_controlled: '1'
scopus_import: '1'
status: public
title: MicroRNAs associated with the different human Argonaute proteins
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 40
year: '2012'
...
---
_id: '3158'
abstract:
- lang: eng
text: We describe here the development and characterization of a conditionally inducible
mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous
actin. We have used this model to study platelets, megakaryocytes and melanoblasts
and we provide evidence that Lifeact-GFP is a useful reporter in these cell types
ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable
by traditional methods, so conditional activation of Lifeact allows the study
of actin dynamics in these cells live. We studied melanoblasts in native skin
explants from embryos, allowing the visualization of live actin dynamics during
cytokinesis and migration. Our study revealed that melanoblasts lacking the small
GTPase Rac1 show a delay in the formation of new pseudopodia following cytokinesis
that accounts for the previously reported cytokinesis delay in these cells. Thus,
through use of this mouse model, we were able to gain insights into the actin
dynamics of cells that could only previously be studied using fixed specimens
or following isolation from their native tissue environment.
article_processing_charge: No
author:
- first_name: Hannah
full_name: Schachtner, Hannah
last_name: Schachtner
- first_name: Ang
full_name: Li, Ang
last_name: Li
- first_name: David
full_name: Stevenson, David
last_name: Stevenson
- first_name: Simon
full_name: Calaminus, Simon
last_name: Calaminus
- first_name: Steven
full_name: Thomas, Steven
last_name: Thomas
- first_name: Steve
full_name: Watson, Steve
last_name: Watson
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Roland
full_name: Wedlich Söldner, Roland
last_name: Wedlich Söldner
- first_name: Douglas
full_name: Strathdee, Douglas
last_name: Strathdee
- first_name: Laura
full_name: Machesky, Laura
last_name: Machesky
citation:
ama: Schachtner H, Li A, Stevenson D, et al. Tissue inducible Lifeact expression
allows visualization of actin dynamics in vivo and ex vivo. European Journal
of Cell Biology. 2012;91(11-12):923-929. doi:10.1016/j.ejcb.2012.04.002
apa: Schachtner, H., Li, A., Stevenson, D., Calaminus, S., Thomas, S., Watson, S.,
… Machesky, L. (2012). Tissue inducible Lifeact expression allows visualization
of actin dynamics in vivo and ex vivo. European Journal of Cell Biology.
Elsevier. https://doi.org/10.1016/j.ejcb.2012.04.002
chicago: Schachtner, Hannah, Ang Li, David Stevenson, Simon Calaminus, Steven Thomas,
Steve Watson, Michael K Sixt, Roland Wedlich Söldner, Douglas Strathdee, and Laura
Machesky. “Tissue Inducible Lifeact Expression Allows Visualization of Actin Dynamics
in Vivo and Ex Vivo.” European Journal of Cell Biology. Elsevier, 2012.
https://doi.org/10.1016/j.ejcb.2012.04.002.
ieee: H. Schachtner et al., “Tissue inducible Lifeact expression allows visualization
of actin dynamics in vivo and ex vivo,” European Journal of Cell Biology,
vol. 91, no. 11–12. Elsevier, pp. 923–929, 2012.
ista: Schachtner H, Li A, Stevenson D, Calaminus S, Thomas S, Watson S, Sixt MK,
Wedlich Söldner R, Strathdee D, Machesky L. 2012. Tissue inducible Lifeact expression
allows visualization of actin dynamics in vivo and ex vivo. European Journal of
Cell Biology. 91(11–12), 923–929.
mla: Schachtner, Hannah, et al. “Tissue Inducible Lifeact Expression Allows Visualization
of Actin Dynamics in Vivo and Ex Vivo.” European Journal of Cell Biology,
vol. 91, no. 11–12, Elsevier, 2012, pp. 923–29, doi:10.1016/j.ejcb.2012.04.002.
short: H. Schachtner, A. Li, D. Stevenson, S. Calaminus, S. Thomas, S. Watson, M.K.
Sixt, R. Wedlich Söldner, D. Strathdee, L. Machesky, European Journal of Cell
Biology 91 (2012) 923–929.
date_created: 2018-12-11T12:01:44Z
date_published: 2012-11-01T00:00:00Z
date_updated: 2025-09-30T07:54:24Z
day: '01'
department:
- _id: MiSi
doi: 10.1016/j.ejcb.2012.04.002
external_id:
isi:
- '000311775000013'
pmid:
- '22658956'
intvolume: ' 91'
isi: 1
issue: 11-12
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930012/
month: '11'
oa: 1
oa_version: Submitted Version
page: 923 - 929
pmid: 1
publication: European Journal of Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '3534'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tissue inducible Lifeact expression allows visualization of actin dynamics
in vivo and ex vivo
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 91
year: '2012'
...
---
OA_type: free access
_id: '3167'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
citation:
ama: Weber M. NextGen speaks 13 . Science. 2012;336(6077):32-34. doi:10.1126/science.336.6077.32
apa: Weber, M. (2012). NextGen speaks 13 . Science. American Association
for the Advancement of Science. https://doi.org/10.1126/science.336.6077.32
chicago: Weber, Michele. “NextGen Speaks 13 .” Science. American Association
for the Advancement of Science, 2012. https://doi.org/10.1126/science.336.6077.32.
ieee: M. Weber, “NextGen speaks 13 ,” Science, vol. 336, no. 6077. American
Association for the Advancement of Science, pp. 32–34, 2012.
ista: Weber M. 2012. NextGen speaks 13 . Science. 336(6077), 32–34.
mla: Weber, Michele. “NextGen Speaks 13 .” Science, vol. 336, no. 6077, American
Association for the Advancement of Science, 2012, pp. 32–34, doi:10.1126/science.336.6077.32.
short: M. Weber, Science 336 (2012) 32–34.
date_created: 2018-12-11T12:01:47Z
date_published: 2012-04-06T00:00:00Z
date_updated: 2025-05-20T07:14:51Z
day: '06'
department:
- _id: MiSi
doi: 10.1126/science.336.6077.32
external_id:
pmid:
- '22491839'
intvolume: ' 336'
issue: '6077'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1126/science.336.6077.32
month: '04'
oa: 1
oa_version: None
page: 32-34
pmid: 1
popular_science: '1'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3516'
status: public
title: 'NextGen speaks 13 '
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 336
year: '2012'
...
---
_id: '506'
article_processing_charge: No
article_type: original
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Sixt MK. Cell migration: Fibroblasts find a new way to get ahead. Journal
of Cell Biology. 2012;197(3):347-349. doi:10.1083/jcb.201204039'
apa: 'Sixt, M. K. (2012). Cell migration: Fibroblasts find a new way to get ahead.
Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.201204039'
chicago: 'Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.”
Journal of Cell Biology. Rockefeller University Press, 2012. https://doi.org/10.1083/jcb.201204039.'
ieee: 'M. K. Sixt, “Cell migration: Fibroblasts find a new way to get ahead,” Journal
of Cell Biology, vol. 197, no. 3. Rockefeller University Press, pp. 347–349,
2012.'
ista: 'Sixt MK. 2012. Cell migration: Fibroblasts find a new way to get ahead. Journal
of Cell Biology. 197(3), 347–349.'
mla: 'Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.”
Journal of Cell Biology, vol. 197, no. 3, Rockefeller University Press,
2012, pp. 347–49, doi:10.1083/jcb.201204039.'
short: M.K. Sixt, Journal of Cell Biology 197 (2012) 347–349.
corr_author: '1'
date_created: 2018-12-11T11:46:51Z
date_published: 2012-04-30T00:00:00Z
date_updated: 2025-09-30T08:24:00Z
day: '30'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1083/jcb.201204039
external_id:
isi:
- '000303467800004'
file:
- access_level: open_access
checksum: 45c02be33ebd99fc3077d60b9c90bdfa
content_type: application/pdf
creator: kschuh
date_created: 2019-02-12T09:03:09Z
date_updated: 2020-07-14T12:46:36Z
file_id: '5957'
file_name: 2012_CellBiology_Sixt.pdf
file_size: 986566
relation: main_file
file_date_updated: 2020-07-14T12:46:36Z
has_accepted_license: '1'
intvolume: ' 197'
isi: 1
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '04'
oa: 1
oa_version: Published Version
page: 347 - 349
publication: Journal of Cell Biology
publication_status: published
publisher: Rockefeller University Press
publist_id: '7314'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Cell migration: Fibroblasts find a new way to get ahead'
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 197
year: '2012'
...
---
_id: '3287'
abstract:
- lang: eng
text: 'Diffusing membrane constituents are constantly exposed to a variety of forces
that influence their stochastic path. Single molecule experiments allow for resolving
trajectories at extremely high spatial and temporal accuracy, thereby offering
insights into en route interactions of the tracer. In this review we discuss approaches
to derive information about the underlying processes, based on single molecule
tracking experiments. In particular, we focus on a new versatile way to analyze
single molecule diffusion in the absence of a full analytical treatment. The method
is based on comprehensive comparison of an experimental data set against the hypothetical
outcome of multiple experiments performed on the computer. Since Monte Carlo simulations
can be easily and rapidly performed even on state-of-the-art PCs, our method provides
a simple way for testing various - even complicated - diffusion models. We describe
the new method in detail, and show the applicability on two specific examples:
firstly, kinetic rate constants can be derived for the transient interaction of
mobile membrane proteins; secondly, residence time and corral size can be extracted
for confined diffusion.'
article_processing_charge: No
author:
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Markus
full_name: Axmann, Markus
last_name: Axmann
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Gerhard
full_name: Schuetz, Gerhard
last_name: Schuetz
citation:
ama: Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule
trajectories? Current Protein & Peptide Science. 2011;12(8):714-724.
doi:10.2174/138920311798841753
apa: Ruprecht, V., Axmann, M., Wieser, S., & Schuetz, G. (2011). What can we
learn from single molecule trajectories? Current Protein & Peptide Science.
Bentham Science Publishers. https://doi.org/10.2174/138920311798841753
chicago: Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What
Can We Learn from Single Molecule Trajectories?” Current Protein & Peptide
Science. Bentham Science Publishers, 2011. https://doi.org/10.2174/138920311798841753.
ieee: V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from
single molecule trajectories?,” Current Protein & Peptide Science,
vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.
ista: Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single
molecule trajectories? Current Protein & Peptide Science. 12(8), 714–724.
mla: Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?”
Current Protein & Peptide Science, vol. 12, no. 8, Bentham Science
Publishers, 2011, pp. 714–24, doi:10.2174/138920311798841753.
short: V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein & Peptide
Science 12 (2011) 714–724.
date_created: 2018-12-11T12:02:28Z
date_published: 2011-12-01T00:00:00Z
date_updated: 2025-09-30T09:21:31Z
day: '01'
department:
- _id: CaHe
- _id: MiSi
doi: 10.2174/138920311798841753
external_id:
isi:
- '000299672600005'
intvolume: ' 12'
isi: 1
issue: '8'
language:
- iso: eng
month: '12'
oa_version: None
page: 714 - 724
publication: Current Protein & Peptide Science
publication_status: published
publisher: Bentham Science Publishers
publist_id: '3358'
quality_controlled: '1'
scopus_import: '1'
status: public
title: What can we learn from single molecule trajectories?
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 12
year: '2011'
...
---
_id: '3371'
abstract:
- lang: eng
text: The Minisymposium “Cell Migration and Motility” was attended by approximately
500 visitors and covered a broad range of questions in the field using diverse
model systems. Topics comprised actin dynamics, cell polarity, force transduction,
signal transduction, bar- rier transmigration, and chemotactic guidance.
article_processing_charge: No
article_type: original
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Carole
full_name: Parent, Carole
last_name: Parent
citation:
ama: Sixt MK, Parent C. Cells on the move in Philadelphia. Molecular Biology
and Evolution. 2011;22(6):724. doi:10.1091/mbc.E10-12-0958
apa: Sixt, M. K., & Parent, C. (2011). Cells on the move in Philadelphia. Molecular
Biology and Evolution. Oxford University Press. https://doi.org/10.1091/mbc.E10-12-0958
chicago: Sixt, Michael K, and Carole Parent. “Cells on the Move in Philadelphia.”
Molecular Biology and Evolution. Oxford University Press, 2011. https://doi.org/10.1091/mbc.E10-12-0958.
ieee: M. K. Sixt and C. Parent, “Cells on the move in Philadelphia,” Molecular
Biology and Evolution, vol. 22, no. 6. Oxford University Press, p. 724, 2011.
ista: Sixt MK, Parent C. 2011. Cells on the move in Philadelphia. Molecular Biology
and Evolution. 22(6), 724.
mla: Sixt, Michael K., and Carole Parent. “Cells on the Move in Philadelphia.” Molecular
Biology and Evolution, vol. 22, no. 6, Oxford University Press, 2011, p. 724,
doi:10.1091/mbc.E10-12-0958.
short: M.K. Sixt, C. Parent, Molecular Biology and Evolution 22 (2011) 724.
date_created: 2018-12-11T12:02:57Z
date_published: 2011-03-15T00:00:00Z
date_updated: 2025-09-30T08:58:51Z
day: '15'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1091/mbc.E10-12-0958
external_id:
isi:
- '000288368200010'
file:
- access_level: open_access
checksum: 3467986ab7a64e7694ffd1013b5d9da9
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:29Z
date_updated: 2020-07-14T12:46:11Z
file_id: '5283'
file_name: IST-2015-373-v1+1_Mol._Biol._Cell-2011-Sixt-724.pdf
file_size: 105421
relation: main_file
file_date_updated: 2020-07-14T12:46:11Z
has_accepted_license: '1'
intvolume: ' 22'
isi: 1
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '724'
publication: Molecular Biology and Evolution
publication_status: published
publisher: Oxford University Press
publist_id: '3238'
pubrep_id: '373'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cells on the move in Philadelphia
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 22
year: '2011'
...
---
_id: '3385'
article_processing_charge: No
article_type: review
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Sixt MK. Interstitial locomotion of leukocytes. Immunology Letters.
2011;138(1):32-34. doi:10.1016/j.imlet.2011.02.013
apa: Sixt, M. K. (2011). Interstitial locomotion of leukocytes. Immunology Letters.
Elsevier. https://doi.org/10.1016/j.imlet.2011.02.013
chicago: Sixt, Michael K. “Interstitial Locomotion of Leukocytes.” Immunology
Letters. Elsevier, 2011. https://doi.org/10.1016/j.imlet.2011.02.013.
ieee: M. K. Sixt, “Interstitial locomotion of leukocytes,” Immunology Letters,
vol. 138, no. 1. Elsevier, pp. 32–34, 2011.
ista: Sixt MK. 2011. Interstitial locomotion of leukocytes. Immunology Letters.
138(1), 32–34.
mla: Sixt, Michael K. “Interstitial Locomotion of Leukocytes.” Immunology Letters,
vol. 138, no. 1, Elsevier, 2011, pp. 32–34, doi:10.1016/j.imlet.2011.02.013.
short: M.K. Sixt, Immunology Letters 138 (2011) 32–34.
corr_author: '1'
date_created: 2018-12-11T12:03:02Z
date_published: 2011-07-01T00:00:00Z
date_updated: 2025-09-30T08:47:13Z
day: '01'
department:
- _id: MiSi
doi: 10.1016/j.imlet.2011.02.013
external_id:
isi:
- '000292714800011'
intvolume: ' 138'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa_version: None
page: 32 - 34
publication: Immunology Letters
publication_status: published
publisher: Elsevier
publist_id: '3222'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Interstitial locomotion of leukocytes
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 138
year: '2011'
...
---
_id: '3392'
abstract:
- lang: eng
text: Migrating lymphocytes acquire a polarized phenotype with a leading and a trailing
edge, or uropod. Although in vitro experiments in cell lines or activated primary
cell cultures have established that Rho-p160 coiled-coil kinase (ROCK)-myosin
II-mediated uropod contractility is required for integrin de-adhesion on two-dimensional
surfaces and nuclear propulsion through narrow pores in three-dimensional matrices,
less is known about the role of these two events during the recirculation of primary,
nonactivated lymphocytes. Using pharmacological antagonists of ROCK and myosin
II, we report that inhibition of uropod contractility blocked integrin-independent
mouse T cell migration through narrow, but not large, pores in vitro. T cell crawling
on chemokine-coated endothelial cells under shear was severely impaired by ROCK
inhibition, whereas transendothelial migration was only reduced through endothelial
cells with high, but not low, barrier properties. Using three-dimensional thick-tissue
imaging and dynamic two-photon microscopy of T cell motility in lymphoid tissue,
we demonstrated a significant role for uropod contractility in intraluminal crawling
and transendothelial migration through lymph node, but not bone marrow, endothelial
cells. Finally, we demonstrated that ICAM-1, but not anatomical constraints or
integrin-independent interactions, reduced parenchymal motility of inhibitor-treated
T cells within the dense lymphoid microenvironment, thus assigning context-dependent
roles for uropod contraction during lymphocyte recirculation.
article_processing_charge: No
article_type: original
author:
- first_name: Silvia
full_name: Soriano, Silvia
last_name: Soriano
- first_name: Miroslav
full_name: Hons, Miroslav
last_name: Hons
orcid: 0000-0002-6625-3348
- first_name: Kathrin
full_name: Schumann, Kathrin
last_name: Schumann
- first_name: Varsha
full_name: Kumar, Varsha
last_name: Kumar
- first_name: Timo
full_name: Dennier, Timo
last_name: Dennier
- first_name: Ruth
full_name: Lyck, Ruth
last_name: Lyck
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Jens
full_name: Stein, Jens
last_name: Stein
citation:
ama: Soriano S, Hons M, Schumann K, et al. In vivo analysis of uropod function during
physiological T cell trafficking. Journal of Immunology. 2011;187(5):2356-2364.
doi:10.4049/jimmunol.1100935
apa: Soriano, S., Hons, M., Schumann, K., Kumar, V., Dennier, T., Lyck, R., … Stein,
J. (2011). In vivo analysis of uropod function during physiological T cell trafficking.
Journal of Immunology. American Association of Immunologists. https://doi.org/10.4049/jimmunol.1100935
chicago: Soriano, Silvia, Miroslav Hons, Kathrin Schumann, Varsha Kumar, Timo Dennier,
Ruth Lyck, Michael K Sixt, and Jens Stein. “In Vivo Analysis of Uropod Function
during Physiological T Cell Trafficking.” Journal of Immunology. American
Association of Immunologists, 2011. https://doi.org/10.4049/jimmunol.1100935.
ieee: S. Soriano et al., “In vivo analysis of uropod function during physiological
T cell trafficking,” Journal of Immunology, vol. 187, no. 5. American Association
of Immunologists, pp. 2356–2364, 2011.
ista: Soriano S, Hons M, Schumann K, Kumar V, Dennier T, Lyck R, Sixt MK, Stein
J. 2011. In vivo analysis of uropod function during physiological T cell trafficking.
Journal of Immunology. 187(5), 2356–2364.
mla: Soriano, Silvia, et al. “In Vivo Analysis of Uropod Function during Physiological
T Cell Trafficking.” Journal of Immunology, vol. 187, no. 5, American Association
of Immunologists, 2011, pp. 2356–64, doi:10.4049/jimmunol.1100935.
short: S. Soriano, M. Hons, K. Schumann, V. Kumar, T. Dennier, R. Lyck, M.K. Sixt,
J. Stein, Journal of Immunology 187 (2011) 2356–2364.
date_created: 2018-12-11T12:03:04Z
date_published: 2011-09-01T00:00:00Z
date_updated: 2025-09-30T08:43:55Z
day: '01'
department:
- _id: MiSi
doi: 10.4049/jimmunol.1100935
external_id:
isi:
- '000294059500040'
intvolume: ' 187'
isi: 1
issue: '5'
language:
- iso: eng
month: '09'
oa_version: None
page: 2356 - 2364
publication: Journal of Immunology
publication_identifier:
eissn:
- 1550-6606
issn:
- 0022-1767
publication_status: published
publisher: American Association of Immunologists
publist_id: '3215'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vivo analysis of uropod function during physiological T cell trafficking
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 187
year: '2011'
...
---
_id: '491'
abstract:
- lang: eng
text: In their search for antigens, lymphocytes continuously shuttle among blood
vessels, lymph vessels, and lymphatic tissues. Chemokines mediate entry of lymphocytes
into lymphatic tissues, and sphingosine 1-phosphate (S1P) promotes localization
of lymphocytes to the vasculature. Both signals are sensed through G protein-coupled
receptors (GPCRs). Most GPCRs undergo ligand-dependent homologous receptor desensitization,
a process that decreases their signaling output after previous exposure to high
ligand concentration. Such desensitization can explain why lymphocytes do not
take an intermediate position between two signals but rather oscillate between
them. The desensitization of S1P receptor 1 (S1PR1) is mediated by GPCR kinase
2 (GRK2). Deletion of GRK2 in lymphocytes compromises desensitization by high
vascular S1P concentrations, thereby reducing responsiveness to the chemokine
signal and trapping the cells in the vascular compartment. The desensitization
kinetics of S1PR1 allows lymphocytes to dynamically shuttle between vasculature
and lymphatic tissue, although the positional information in both compartments
is static.
article_number: pe43
article_processing_charge: No
author:
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Eichner A, Sixt MK. Setting the clock for recirculating lymphocytes. Science
Signaling. 2011;4(198). doi:10.1126/scisignal.2002617
apa: Eichner, A., & Sixt, M. K. (2011). Setting the clock for recirculating
lymphocytes. Science Signaling. American Association for the Advancement
of Science. https://doi.org/10.1126/scisignal.2002617
chicago: Eichner, Alexander, and Michael K Sixt. “Setting the Clock for Recirculating
Lymphocytes.” Science Signaling. American Association for the Advancement
of Science, 2011. https://doi.org/10.1126/scisignal.2002617.
ieee: A. Eichner and M. K. Sixt, “Setting the clock for recirculating lymphocytes,”
Science Signaling, vol. 4, no. 198. American Association for the Advancement
of Science, 2011.
ista: Eichner A, Sixt MK. 2011. Setting the clock for recirculating lymphocytes.
Science Signaling. 4(198), pe43.
mla: Eichner, Alexander, and Michael K. Sixt. “Setting the Clock for Recirculating
Lymphocytes.” Science Signaling, vol. 4, no. 198, pe43, American Association
for the Advancement of Science, 2011, doi:10.1126/scisignal.2002617.
short: A. Eichner, M.K. Sixt, Science Signaling 4 (2011).
corr_author: '1'
date_created: 2018-12-11T11:46:46Z
date_published: 2011-11-08T00:00:00Z
date_updated: 2025-09-30T09:24:17Z
day: '08'
department:
- _id: MiSi
doi: 10.1126/scisignal.2002617
external_id:
isi:
- '000296800500002'
intvolume: ' 4'
isi: 1
issue: '198'
language:
- iso: eng
month: '11'
oa_version: None
publication: Science Signaling
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '7329'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Setting the clock for recirculating lymphocytes
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 4
year: '2011'
...
---
_id: '518'
abstract:
- lang: eng
text: Cancer stem cells or cancer initiating cells are believed to contribute to
cancer recurrence after therapy. MicroRNAs (miRNAs) are short RNA molecules with
fundamental roles in gene regulation. The role of miRNAs in cancer stem cells
is only poorly understood. Here, we report miRNA expression profiles of glioblastoma
stem cell-containing CD133 + cell populations. We find that miR-9, miR-9 * (referred
to as miR-9/9 *), miR-17 and miR-106b are highly abundant in CD133 + cells. Furthermore,
inhibition of miR-9/9 * or miR-17 leads to reduced neurosphere formation and stimulates
cell differentiation. Calmodulin-binding transcription activator 1 (CAMTA1) is
a putative transcription factor, which induces the expression of the anti-proliferative
cardiac hormone natriuretic peptide A (NPPA). We identify CAMTA1 as an miR-9/9
* and miR-17 target. CAMTA1 expression leads to reduced neurosphere formation
and tumour growth in nude mice, suggesting that CAMTA1 can function as tumour
suppressor. Consistently, CAMTA1 and NPPA expression correlate with patient survival.
Our findings could provide a basis for novel strategies of glioblastoma therapy.
article_processing_charge: No
article_type: original
author:
- first_name: Daniel
full_name: Schraivogel, Daniel
last_name: Schraivogel
- first_name: Lasse
full_name: Weinmann, Lasse
last_name: Weinmann
- first_name: Dagmar
full_name: Beier, Dagmar
last_name: Beier
- first_name: Ghazaleh
full_name: Tabatabai, Ghazaleh
last_name: Tabatabai
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Jia
full_name: Zhu, Jia
last_name: Zhu
- first_name: Martina
full_name: Anton, Martina
last_name: Anton
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Michael
full_name: Weller, Michael
last_name: Weller
- first_name: Christoph
full_name: Beier, Christoph
last_name: Beier
- first_name: Gunter
full_name: Meister, Gunter
last_name: Meister
citation:
ama: Schraivogel D, Weinmann L, Beier D, et al. CAMTA1 is a novel tumour suppressor
regulated by miR-9/9 * in glioblastoma stem cells. EMBO Journal. 2011;30(20):4309-4322.
doi:10.1038/emboj.2011.301
apa: Schraivogel, D., Weinmann, L., Beier, D., Tabatabai, G., Eichner, A., Zhu,
J., … Meister, G. (2011). CAMTA1 is a novel tumour suppressor regulated by miR-9/9
* in glioblastoma stem cells. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2011.301
chicago: Schraivogel, Daniel, Lasse Weinmann, Dagmar Beier, Ghazaleh Tabatabai,
Alexander Eichner, Jia Zhu, Martina Anton, et al. “CAMTA1 Is a Novel Tumour Suppressor
Regulated by MiR-9/9 * in Glioblastoma Stem Cells.” EMBO Journal. Wiley-Blackwell,
2011. https://doi.org/10.1038/emboj.2011.301.
ieee: D. Schraivogel et al., “CAMTA1 is a novel tumour suppressor regulated
by miR-9/9 * in glioblastoma stem cells,” EMBO Journal, vol. 30, no. 20.
Wiley-Blackwell, pp. 4309–4322, 2011.
ista: Schraivogel D, Weinmann L, Beier D, Tabatabai G, Eichner A, Zhu J, Anton M,
Sixt MK, Weller M, Beier C, Meister G. 2011. CAMTA1 is a novel tumour suppressor
regulated by miR-9/9 * in glioblastoma stem cells. EMBO Journal. 30(20), 4309–4322.
mla: Schraivogel, Daniel, et al. “CAMTA1 Is a Novel Tumour Suppressor Regulated
by MiR-9/9 * in Glioblastoma Stem Cells.” EMBO Journal, vol. 30, no. 20,
Wiley-Blackwell, 2011, pp. 4309–22, doi:10.1038/emboj.2011.301.
short: D. Schraivogel, L. Weinmann, D. Beier, G. Tabatabai, A. Eichner, J. Zhu,
M. Anton, M.K. Sixt, M. Weller, C. Beier, G. Meister, EMBO Journal 30 (2011) 4309–4322.
date_created: 2018-12-11T11:46:55Z
date_published: 2011-10-19T00:00:00Z
date_updated: 2025-09-30T09:23:51Z
day: '19'
department:
- _id: MiSi
doi: 10.1038/emboj.2011.301
external_id:
isi:
- '000296715800018'
pmid:
- '21857646'
intvolume: ' 30'
isi: 1
issue: '20'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199389/
month: '10'
oa: 1
oa_version: Submitted Version
page: 4309 - 4322
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '7301'
quality_controlled: '1'
scopus_import: '1'
status: public
title: CAMTA1 is a novel tumour suppressor regulated by miR-9/9 * in glioblastoma
stem cells
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 30
year: '2011'
...
---
_id: '3505'
abstract:
- lang: eng
text: Cell migration on two-dimensional (2D) substrates follows entirely different
rules than cell migration in three-dimensional (3D) environments. This is especially
relevant for leukocytes that are able to migrate in the absence of adhesion receptors
within the confined geometry of artificial 3D extracellular matrix scaffolds and
within the interstitial space in vivo. Here, we describe in detail a simple and
economical protocol to visualize dendritic cell migration in 3D collagen scaffolds
along chemotactic gradients. This method can be adapted to other cell types and
may serve as a physiologically relevant paradigm for the directed locomotion of
most amoeboid cells.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
article_type: original
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Tim
full_name: Lämmermann, Tim
last_name: Lämmermann
citation:
ama: Sixt MK, Lämmermann T. In vitro analysis of chemotactic leukocyte migration
in 3D environments. Cell Migration. 2011;769:149-165. doi:10.1007/978-1-61779-207-6_11
apa: Sixt, M. K., & Lämmermann, T. (2011). In vitro analysis of chemotactic
leukocyte migration in 3D environments. Cell Migration. Springer. https://doi.org/10.1007/978-1-61779-207-6_11
chicago: Sixt, Michael K, and Tim Lämmermann. “In Vitro Analysis of Chemotactic
Leukocyte Migration in 3D Environments.” Cell Migration. Springer, 2011.
https://doi.org/10.1007/978-1-61779-207-6_11.
ieee: M. K. Sixt and T. Lämmermann, “In vitro analysis of chemotactic leukocyte
migration in 3D environments,” Cell Migration, vol. 769. Springer, pp.
149–165, 2011.
ista: Sixt MK, Lämmermann T. 2011. In vitro analysis of chemotactic leukocyte migration
in 3D environments. Cell Migration. 769, 149–165.
mla: Sixt, Michael K., and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte
Migration in 3D Environments.” Cell Migration, vol. 769, Springer, 2011,
pp. 149–65, doi:10.1007/978-1-61779-207-6_11.
short: M.K. Sixt, T. Lämmermann, Cell Migration 769 (2011) 149–165.
corr_author: '1'
date_created: 2018-12-11T12:03:41Z
date_published: 2011-05-17T00:00:00Z
date_updated: 2024-10-21T06:03:02Z
day: '17'
department:
- _id: MiSi
doi: 10.1007/978-1-61779-207-6_11
intvolume: ' 769'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://pure.mpg.de/pubman/item/item_3219628_1/component/file_3219630/Sixt%20et%20al..pdf
month: '05'
oa: 1
oa_version: Published Version
page: 149 - 165
publication: Cell Migration
publication_status: published
publisher: Springer
publist_id: '2882'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro analysis of chemotactic leukocyte migration in 3D environments
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 769
year: '2011'
...
---
OA_place: publisher
_id: '3275'
abstract:
- lang: eng
text: 'Chemokines organize immune cell trafficking by inducing either directed (tactic)
or random (kinetic) migration and by activating integrins in order to support
surface adhesion (haptic). Beyond that the same chemokines can establish clearly
defined functional areas in secondary lymphoid organs. Until now it is unclear
how chemokines can fulfill such diverse functions. One decisive prerequisite to
explain these capacities is to know how chemokines are presented in tissue. In
theory chemokines could occur either soluble or immobilized, and could be distributed
either homogenously or as a concentration gradient. To dissect if and how the
presenting mode of chemokines influences immune cells, I tested the response of
dendritic cells (DCs) to differentially displayed chemokines. DCs are antigen
presenting cells that reside in the periphery and migrate into draining lymph
nodes (LNs) once exposed to inflammatory stimuli to activate naïve T cells. DCs
are guided to and within the LN by the chemokine receptor CCR7, which has two
ligands, the chemokines CCL19 and CCL21. Both CCR7 ligands are expressed by fibroblastic
reticular cells in the LN, but differ in their ability to bind to heparan sulfate
residues. CCL21 has a highly charged C-terminal extension, which mediates binding
to anionic surfaces, whereas CCL19 is lacking such residues and likely distributes
as a soluble molecule. This study shows that surface-bound CCL21 causes random,
haptokinetic DC motility, which is confined to the chemokine coated area by insideout
activation of β2 integrins that mediate cell binding to the surface. CCL19 on
the other hand forms concentration gradients which trigger directional, chemotactic
movement, but no surface adhesion. In addition DCs can actively manipulate this
system by recruiting and activating serine proteases on their surfaces, which
create - by proteolytically removing the adhesive C-terminus - a solubilized variant
of CCL21 that functionally resembles CCL19. By generating a CCL21 concentration
gradient DCs establish a positive feedback loop to recruit further DCs from the
periphery to the CCL21 coated region. In addition DCs can sense chemotactic gradients
as well as immobilized haptokinetic fields at the same time and integrate these
signals. The result is chemotactically biased haptokinesis - directional migration
confined to a chemokine coated track or area - which could explain the dynamic
but spatially tightly controlled swarming leukocyte locomotion patterns that have
been observed in lymphatic organs by intravital microscopists. The finding that
DCs can approach soluble cues in a non-adhesive manner while they attach to surfaces
coated with immobilized cues raises the question how these cells transmit intracellular
forces to the environment, especially in the non-adherent migration mode. In order
to migrate, cells have to generate and transmit force to the extracellular substrate.
Force transmission is the prerequisite to procure an expansion of the leading
edge and a forward motion of the whole cell body. In the current conceptions actin
polymerization at the leading edge is coupled to extracellular ligands via the
integrin family of transmembrane receptors, which allows the transmission of intracellular
force. Against the paradigm of force transmission during migration, leukocytes,
like DCs, are able to migrate in threedimensional environments without using integrin
transmembrane receptors (Lämmermann et al., 2008). This reflects the biological
function of leukocytes, as they can invade almost all tissues, whereby their migration
has to be independent from the extracellular environment. How the cells can achieve
this is unclear. For this study I examined DC migration in a defined threedimensional
environment and highlighted actin-dynamics with the probe Lifeact-GFP. The result
was that chemotactic DCs can switch between integrin-dependent and integrin- independent
locomotion and can thereby adapt to the adhesive properties of their environment.
If the cells are able to couple their actin cytoskeleton to the substrate, actin
polymerization is entirely converted into protrusion. Without coupling the actin
cortex undergoes slippage and retrograde actin flow can be observed. But retrograde
actin flow can be completely compensated by higher actin polymerization rate keeping
the migration velocity and the shape of the cells unaltered. Mesenchymal cells
like fibroblast cannot balance the loss of adhesive interaction, cannot protrude
into open space and, therefore, strictly depend on integrinmediated force coupling.
This leukocyte specific phenomenon of “adaptive force transmission” endows these
cells with the unique ability to transit and invade almost every type of tissue. '
acknowledgement: "I would like to express my sincere gratitude to the following people
who made with their continuous support and encouragement this thesis possible: First,
I want to thank Prof. Dr. Michael Sixt for his excellent supervision and mentoring,
especially for the nice, relaxed working atmosphere, a lot of brilliant ideas and
the freedom to work in my own way.\r\n\r\nProf. Dr. Reinhard Fässler for his constant
support of the Sixt lab and for providing excellent working conditions. \r\n\r\nProf.
Dr. Sanjiv Luther and Prof. Dr. Tobias Bollenbach for agreeing to be member of my
thesis committee and to evaluate my work.\r\n\r\nDr. Walther Göhring, Carmen Schmitz,
the Recombinant Protein Production core facility and the animal care takers for
providing the “infrastructure” for this thesis. \r\n\r\nProf. Dr. Daniel Legler,
Markus Bruckner and Dr. Julien Polleux for very fruitful collaborations and discussions.\r\n\r\nMy
labmates for their help, a lot of discussions and to make the Sixt lab to a convenient
place to work : Karin Hirsch, Tim Lämmeramnn, Holger Pflicke, Jörg Renkawitz, Michele
Weber and Alexander Eichner All members of the Department of Molecular Medicine
for their help. Especially I want to thank Sarah Schmidt, Karin Hirsch and Raphael
Ruppert for their friendship, nice chats and their uncensored point of view. "
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Kathrin
full_name: Schumann, Kathrin
id: F44D762E-4F9D-11E9-B64C-9EB26CEFFB5F
last_name: Schumann
citation:
ama: Schumann K. The role of chemotactic gradients in dendritic cell migration.
2011.
apa: Schumann, K. (2011). The role of chemotactic gradients in dendritic cell
migration. Institute of Science and Technology Austria.
chicago: Schumann, Kathrin. “The Role of Chemotactic Gradients in Dendritic Cell
Migration.” Institute of Science and Technology Austria, 2011.
ieee: K. Schumann, “The role of chemotactic gradients in dendritic cell migration,”
Institute of Science and Technology Austria, 2011.
ista: Schumann K. 2011. The role of chemotactic gradients in dendritic cell migration.
Institute of Science and Technology Austria.
mla: Schumann, Kathrin. The Role of Chemotactic Gradients in Dendritic Cell Migration.
Institute of Science and Technology Austria, 2011.
short: K. Schumann, The Role of Chemotactic Gradients in Dendritic Cell Migration,
Institute of Science and Technology Austria, 2011.
corr_author: '1'
date_created: 2018-12-11T12:02:24Z
date_published: 2011-03-01T00:00:00Z
date_updated: 2026-04-09T14:36:24Z
day: '01'
ddc:
- '570'
- '579'
degree_awarded: PhD
department:
- _id: MiSi
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language:
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month: '03'
oa: 1
oa_version: Published Version
page: '141'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '3371'
pubrep_id: '11'
status: public
supervisor:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
title: The role of chemotactic gradients in dendritic cell migration
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2011'
...