[{"has_accepted_license":"1","OA_type":"gold","volume":12,"ddc":["570"],"publication_status":"published","day":"05","article_processing_charge":"Yes (via OA deal)","citation":{"short":"J. Arruda, E. Alamoudi, R. Mueller, M. Vaisband, R. Molkenbur, J. Merrin, E. Kiermaier, J. Hasenauer, Npj Systems Biology and Applications 12 (2026).","mla":"Arruda, Jonas, et al. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” <i>Npj Systems Biology and Applications</i>, vol. 12, 20, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41540-026-00648-9\">10.1038/s41540-026-00648-9</a>.","ista":"Arruda J, Alamoudi E, Mueller R, Vaisband M, Molkenbur R, Merrin J, Kiermaier E, Hasenauer J. 2026. Simulation-based inference of cell migration dynamics in complex spatial environments. npj Systems Biology and Applications. 12, 20.","ama":"Arruda J, Alamoudi E, Mueller R, et al. Simulation-based inference of cell migration dynamics in complex spatial environments. <i>npj Systems Biology and Applications</i>. 2026;12. doi:<a href=\"https://doi.org/10.1038/s41540-026-00648-9\">10.1038/s41540-026-00648-9</a>","chicago":"Arruda, Jonas, Emad Alamoudi, Robert Mueller, Marc Vaisband, Ronja Molkenbur, Jack Merrin, Eva Kiermaier, and Jan Hasenauer. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” <i>Npj Systems Biology and Applications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41540-026-00648-9\">https://doi.org/10.1038/s41540-026-00648-9</a>.","ieee":"J. Arruda <i>et al.</i>, “Simulation-based inference of cell migration dynamics in complex spatial environments,” <i>npj Systems Biology and Applications</i>, vol. 12. Springer Nature, 2026.","apa":"Arruda, J., Alamoudi, E., Mueller, R., Vaisband, M., Molkenbur, R., Merrin, J., … Hasenauer, J. (2026). Simulation-based inference of cell migration dynamics in complex spatial environments. <i>Npj Systems Biology and Applications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41540-026-00648-9\">https://doi.org/10.1038/s41540-026-00648-9</a>"},"date_published":"2026-02-05T00:00:00Z","quality_controlled":"1","intvolume":"        12","OA_place":"publisher","date_updated":"2026-02-23T10:10:10Z","author":[{"last_name":"Arruda","full_name":"Arruda, Jonas","first_name":"Jonas"},{"last_name":"Alamoudi","first_name":"Emad","full_name":"Alamoudi, Emad"},{"last_name":"Mueller","first_name":"Robert","full_name":"Mueller, Robert"},{"first_name":"Marc","full_name":"Vaisband, Marc","last_name":"Vaisband"},{"last_name":"Molkenbur","full_name":"Molkenbur, Ronja","first_name":"Ronja"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kiermaier, Eva","first_name":"Eva","last_name":"Kiermaier"},{"full_name":"Hasenauer, Jan","first_name":"Jan","last_name":"Hasenauer"}],"oa":1,"file":[{"content_type":"application/pdf","file_size":10217687,"date_updated":"2026-02-23T10:09:03Z","success":1,"relation":"main_file","creator":"dernst","file_id":"21346","checksum":"99b2e6bbaaedf45f22e07751948669f5","file_name":"2026_npjSysBioApp_Arruda.pdf","date_created":"2026-02-23T10:09:03Z","access_level":"open_access"}],"status":"public","language":[{"iso":"eng"}],"publication":"npj Systems Biology and Applications","date_created":"2026-02-16T10:44:31Z","publisher":"Springer Nature","article_type":"original","PlanS_conform":"1","oa_version":"Published Version","article_number":"20","acknowledgement":"This work was supported by the German Federal Ministry of Education and Research (BMBF) (EMUNE/031L0293C), the European Union via the ERC grant INTEGRATE, grant agreement number 101126146, and under Germany’s Excellence Strategy by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (EXC 2047—390685813, EXC 2151—390873048, FOR5775 — 533863915, and 524747443), the University of Bonn via the Schlegel Professorship of J.H., and the returning experts fellowship of the Ministry of Innovation, Science, and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069). J.M. is a member of the Nanofabrication Facility and is supported by the Institute of Science and Technology Austria. E.K. acknowledges the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments. The authors thank Laeschkir Würthner for his insightful comments on the implementation of the authors’ model. The views and opinions expressed are those of the authors only and do not necessarily reflect those of the funding agencies. Parts of Fig. 1 were created using BioRender. Open Access funding enabled and organized by Projekt DEAL.","pmid":1,"abstract":[{"lang":"eng","text":"To assess cell migration in complex spatial environments, microfabricated chips, such as mazes and pillar forests, are routinely used to impose spatial and mechanical constraints, and cell trajectories are followed within these structures by advanced imaging techniques. In systems mechanobiology, computational models serve as essential tools to uncover how physical geometry influences intracellular dynamics; however, decoding such complex behaviors requires advanced inference techniques. Here, we integrated experimental observations of dendritic cell migration in a geometrically constrained microenvironment into a Cellular Potts model. We demonstrated that these spatial constraints modulate the motility dynamics, including speed and directional changes. We show that classical summary statistics, such as mean squared displacement and turning angle distributions, can resolve key mechanistic features but fail to extract richer spatiotemporal patterns, limiting accurate parameter inference. To solve this, we applied neural posterior estimation with in-the-loop learning of summary features. This learned summary representation of the data enables robust and flexible parameter inference, providing a data-driven framework for model calibration and advancing quantitative analysis of cell migration in structured microenvironments."}],"DOAJ_listed":"1","title":"Simulation-based inference of cell migration dynamics in complex spatial environments","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2026","external_id":{"pmid":["41611727"]},"type":"journal_article","month":"02","_id":"21231","department":[{"_id":"NanoFab"}],"file_date_updated":"2026-02-23T10:09:03Z","scopus_import":"1","publication_identifier":{"eissn":["2056-7189"]},"doi":"10.1038/s41540-026-00648-9"},{"_id":"19663","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"file_date_updated":"2025-05-12T07:46:10Z","scopus_import":"1","publication_identifier":{"eissn":["2375-2548"]},"issue":"17","doi":"10.1126/sciadv.adx4047","article_number":"eadx4047","acknowledgement":"We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids; M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids; M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts, Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support; M. Benjamin Braun for critical reading of the manuscript; and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.); German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (J.R.); Medical & Clinician Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048 (D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag 457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.); Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments (E.K.); and CZI grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (R.H.).","pmid":1,"DOAJ_listed":"1","abstract":[{"text":"The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules that grow radially and exert forces on cargos. At the same time, mechanical stresses from the microenvironment and cellular shape changes compress and bend microtubules. Yet, centrosomes are membraneless organelles, raising the question of how centrosomes withstand mechanical forces. Here, we discover that centrosomes can deform and even fracture. We reveal that centrosomes experience deformations during navigational pathfinding within motile cells. Coherence of the centrosome is maintained by Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes cellular function by generating coexisting microtubule organizing centers that compete during path navigation and thereby cause cellular entanglement in the microenvironment. Our findings show that cells actively maintain the integrity of the centrosome to withstand mechanical forces. These results suggest that centrosome stability preservation is fundamental, given that almost all cells in multicellular organisms experience forces.","lang":"eng"}],"title":"Protecting centrosomes from fracturing enables efficient cell navigation","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2025","external_id":{"pmid":["40279414"],"isi":["001476113400016"]},"type":"journal_article","month":"04","quality_controlled":"1","intvolume":"        11","OA_place":"publisher","date_updated":"2025-09-30T12:26:21Z","author":[{"first_name":"Madeleine T.","full_name":"Schmitt, Madeleine T.","last_name":"Schmitt"},{"full_name":"Kroll, Janina","first_name":"Janina","last_name":"Kroll"},{"first_name":"Mauricio J.A.","full_name":"Ruiz-Fernandez, Mauricio J.A.","last_name":"Ruiz-Fernandez"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522"},{"first_name":"Shaunak","full_name":"Ghosh, Shaunak","last_name":"Ghosh"},{"last_name":"Kameritsch","full_name":"Kameritsch, Petra","first_name":"Petra"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack"},{"first_name":"Johanna","full_name":"Schmid, Johanna","last_name":"Schmid"},{"full_name":"Stefanowski, Kasia","first_name":"Kasia","last_name":"Stefanowski"},{"last_name":"Thomae","full_name":"Thomae, Andreas W.","first_name":"Andreas W."},{"full_name":"Cheng, Jingyuan","first_name":"Jingyuan","last_name":"Cheng"},{"first_name":"Gamze Naz","full_name":"Öztan, Gamze Naz","last_name":"Öztan"},{"last_name":"Konopka","full_name":"Konopka, Peter","first_name":"Peter"},{"full_name":"Ortega, Germán Camargo","first_name":"Germán Camargo","last_name":"Ortega"},{"last_name":"Penz","first_name":"Thomas","full_name":"Penz, Thomas"},{"last_name":"Bach","full_name":"Bach, Luisa","first_name":"Luisa"},{"first_name":"Dirk","full_name":"Baumjohann, Dirk","last_name":"Baumjohann"},{"full_name":"Bock, Christoph","first_name":"Christoph","last_name":"Bock"},{"last_name":"Straub","first_name":"Tobias","full_name":"Straub, Tobias"},{"last_name":"Meissner","first_name":"Felix","full_name":"Meissner, Felix"},{"last_name":"Kiermaier","orcid":"0000-0001-6165-5738","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva","first_name":"Eva"},{"last_name":"Renkawitz","orcid":"0000-0003-2856-3369","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","first_name":"Jörg","full_name":"Renkawitz, Jörg"}],"oa":1,"isi":1,"file":[{"date_created":"2025-05-12T07:46:10Z","access_level":"open_access","file_id":"19679","file_name":"2025_ScienceAdvance_Schmitt.pdf","checksum":"e8ba22922fa5b23ccfcce8865f57226c","date_updated":"2025-05-12T07:46:10Z","success":1,"relation":"main_file","creator":"dernst","content_type":"application/pdf","file_size":2707050}],"status":"public","language":[{"iso":"eng"}],"publication":"Science Advances","date_created":"2025-05-11T22:02:38Z","publisher":"AAAS","article_type":"original","oa_version":"Published Version","has_accepted_license":"1","OA_type":"gold","volume":11,"ddc":["570"],"publication_status":"published","day":"25","article_processing_charge":"Yes","citation":{"short":"M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P. Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N. Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub, F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).","mla":"Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>, vol. 11, no. 17, eadx4047, AAAS, 2025, doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>.","ama":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. 2025;11(17). doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>","ista":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P, Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 11(17), eadx4047.","chicago":"Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>. AAAS, 2025. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>.","apa":"Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh, S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>","ieee":"M. T. Schmitt <i>et al.</i>, “Protecting centrosomes from fracturing enables efficient cell navigation,” <i>Science Advances</i>, vol. 11, no. 17. AAAS, 2025."},"date_published":"2025-04-25T00:00:00Z","project":[{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"}]},{"month":"08","type":"journal_article","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"year":"2025","abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"pmid":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","title":"Migrating immune cells globally coordinate protrusive forces","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","scopus_import":"1","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"doi":"10.1038/s41590-025-02211-w","file_date_updated":"2025-07-31T08:00:33Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"_id":"20082","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"date_published":"2025-08-01T00:00:00Z","project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"},{"name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","grant_number":"944-2020"}],"corr_author":"1","volume":26,"OA_type":"hybrid","citation":{"apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266."},"article_processing_charge":"Yes (via OA deal)","publication_status":"published","ddc":["570"],"day":"01","has_accepted_license":"1","PlanS_conform":"1","article_type":"letter_note","related_material":{"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/bench-pressing-cells/","relation":"press_release"}],"record":[{"id":"20149","status":"public","relation":"dissertation_contains"}]},"oa_version":"Published Version","page":"1258–1266","publication":"Nature Immunology","language":[{"iso":"eng"}],"date_created":"2025-07-27T22:01:26Z","publisher":"Springer Nature","isi":1,"file":[{"content_type":"application/pdf","file_size":13514646,"success":1,"date_updated":"2025-07-31T08:00:33Z","relation":"main_file","creator":"dernst","file_id":"20096","file_name":"2025_NatureImmunology_ReisRodrigues.pdf","checksum":"0c725123dca7797c682609bff2c4c5ac","date_created":"2025-07-31T08:00:33Z","access_level":"open_access"}],"oa":1,"author":[{"last_name":"Dos Reis Rodrigues","orcid":"0000-0003-1681-508X","first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F"},{"last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X","first_name":"Mario","full_name":"Avellaneda Sarrió, Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"full_name":"Canigova, Nikola","first_name":"Nikola","id":"3795523E-F248-11E8-B48F-1D18A9856A87","last_name":"Canigova","orcid":"0000-0002-8518-5926"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","last_name":"Gärtner"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","full_name":"Vaahtomeri, Kari","first_name":"Kari","orcid":"0000-0001-7829-3518","last_name":"Vaahtomeri"},{"last_name":"Riedl","orcid":"0000-0003-4844-6311","full_name":"Riedl, Michael","first_name":"Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","first_name":"Ingrid","last_name":"De Vries"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"last_name":"Hauschild","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert"},{"last_name":"Fukui","full_name":"Fukui, Yoshinori","first_name":"Yoshinori"},{"last_name":"Juanes Garcia","orcid":"0000-0002-1009-9652","id":"40F05888-F248-11E8-B48F-1D18A9856A87","full_name":"Juanes Garcia, Alba","first_name":"Alba"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K"}],"status":"public","quality_controlled":"1","intvolume":"        26","date_updated":"2026-04-28T13:26:50Z","OA_place":"publisher"},{"has_accepted_license":"1","project":[{"name":"Tribocharge: a multi-scale approach to an enduring problem in physics","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","call_identifier":"H2020","grant_number":"949120"}],"date_published":"2025-10-01T00:00:00Z","corr_author":"1","volume":12,"OA_type":"gold","article_processing_charge":"Yes","citation":{"chicago":"Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” <i>Advanced Materials Interfaces</i>. Wiley, 2025. <a href=\"https://doi.org/10.1002/admi.202500521\">https://doi.org/10.1002/admi.202500521</a>.","ieee":"I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between surface charge and work function in scanning Kelvin probe microscopy,” <i>Advanced Materials Interfaces</i>, vol. 12, no. 19. Wiley, 2025.","apa":"Lenton, I. C., Pertl, F., Shafeek, L. B., &#38; Waitukaitis, S. R. (2025). A duality between surface charge and work function in scanning Kelvin probe microscopy. <i>Advanced Materials Interfaces</i>. Wiley. <a href=\"https://doi.org/10.1002/admi.202500521\">https://doi.org/10.1002/admi.202500521</a>","ista":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 12(19), e00521.","ama":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge and work function in scanning Kelvin probe microscopy. <i>Advanced Materials Interfaces</i>. 2025;12(19). doi:<a href=\"https://doi.org/10.1002/admi.202500521\">10.1002/admi.202500521</a>","mla":"Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” <i>Advanced Materials Interfaces</i>, vol. 12, no. 19, e00521, Wiley, 2025, doi:<a href=\"https://doi.org/10.1002/admi.202500521\">10.1002/admi.202500521</a>.","short":"I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials Interfaces 12 (2025)."},"ddc":["530"],"day":"01","publication_status":"published","file":[{"date_created":"2025-12-30T09:31:11Z","access_level":"open_access","file_id":"20908","file_name":"2025_AdvMaterialsInterfaces_Lenton.pdf","checksum":"906fcc7733be8ce8a83600427b82cd5a","date_updated":"2025-12-30T09:31:11Z","success":1,"relation":"main_file","creator":"dernst","file_size":1830117,"content_type":"application/pdf"}],"isi":1,"author":[{"first_name":"Isaac C","full_name":"Lenton, Isaac C","id":"a550210f-223c-11ec-8182-e2d45e817efb","orcid":"0000-0002-5010-6984","last_name":"Lenton"},{"last_name":"Pertl","orcid":"0000-0003-0463-5794","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","full_name":"Pertl, Felix","first_name":"Felix"},{"id":"3CD37A82-F248-11E8-B48F-1D18A9856A87","first_name":"Lubuna B","full_name":"Shafeek, Lubuna B","orcid":"0000-0001-7180-6050","last_name":"Shafeek"},{"id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","first_name":"Scott R","full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176","last_name":"Waitukaitis"}],"oa":1,"status":"public","intvolume":"        12","quality_controlled":"1","date_updated":"2025-12-30T09:31:25Z","OA_place":"publisher","PlanS_conform":"1","article_type":"original","oa_version":"Published Version","arxiv":1,"publication":"Advanced Materials Interfaces","language":[{"iso":"eng"}],"publisher":"Wiley","date_created":"2025-09-07T22:01:33Z","DOAJ_listed":"1","abstract":[{"text":"Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic imaging of the electrostatic potential above a surface. Though most often used to image work-function variations of conductive surfaces, it can also be used to probe the surface charge on insulating surfaces. In both cases, relating the measured potential to the underlying signal is non-trivial. Here, general relationships are derived between the measured SKPM voltage and the underlying source, revealing either can be cast as a convolution with an appropriately scaled point spread function (PSF). For charge that exists on a thin insulating layer above a conductor, the PSF has the same shape as what would occur from a work-function variation alone, differing by a simple scaling factor. This relationship is confirmed by: (1) backing it out from finite-element simulations of work-function and charge signals, and (2) experimentally comparing the measured PSF from a small work-function target to that from a small charge spot. This scaling factor is further validated by comparing SKPM charge measurements with Faraday cup measurements for highly charged samples from contact-charging experiments. These results highlight a heretofore unappreciated connection between SKPM voltage and charge signals, offering a rigorous recipe to extract either from experimental data.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"A duality between surface charge and work function in scanning Kelvin probe microscopy","article_number":"e00521","acknowledgement":"This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant agreement No. 949120). This research was supported by the Scientific Service Units of The Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions of the whole Waitukaitis group for useful discussions and feedback.","month":"10","type":"journal_article","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"external_id":{"isi":["001560163400001"],"arxiv":["2506.07187"]},"year":"2025","_id":"20295","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"department":[{"_id":"ScWa"},{"_id":"NanoFab"}],"scopus_import":"1","doi":"10.1002/admi.202500521","publication_identifier":{"eissn":["2196-7350"]},"issue":"19","ec_funded":1,"file_date_updated":"2025-12-30T09:31:11Z"},{"doi":"10.1016/j.devcel.2025.10.006","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"scopus_import":"1","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"acknowledged_ssus":[{"_id":"NanoFab"}],"_id":"20859","external_id":{"pmid":["41192429"]},"year":"2025","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"month":"11","type":"journal_article","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","abstract":[{"lang":"eng","text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo."}],"pmid":1,"date_created":"2025-12-28T23:01:27Z","publisher":"Elsevier","publication":"Developmental Cell","language":[{"iso":"eng"}],"oa_version":"Published Version","PlanS_conform":"1","article_type":"original","date_updated":"2025-12-29T09:23:58Z","OA_place":"publisher","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2025.10.006"}],"quality_controlled":"1","status":"public","author":[{"first_name":"Iván","full_name":"Company-Garrido, Iván","last_name":"Company-Garrido"},{"last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto","first_name":"Alberto"},{"first_name":"Mariona","full_name":"Colomer-Rosell, Mariona","last_name":"Colomer-Rosell"},{"last_name":"Ciraulo","full_name":"Ciraulo, Bernard","first_name":"Bernard"},{"full_name":"Molkenbur, Ronja","first_name":"Ronja","last_name":"Molkenbur"},{"first_name":"Peter","full_name":"Lanzerstorfer, Peter","last_name":"Lanzerstorfer"},{"full_name":"Pezzano, Fabio","first_name":"Fabio","last_name":"Pezzano"},{"last_name":"Agazzi","first_name":"Costanza","full_name":"Agazzi, Costanza"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","first_name":"Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild"},{"full_name":"Jain, Saumey","first_name":"Saumey","last_name":"Jain"},{"last_name":"Jacques","full_name":"Jacques, Jeroen M.","first_name":"Jeroen M."},{"full_name":"Venturini, Valeria","first_name":"Valeria","last_name":"Venturini"},{"last_name":"Knapp","first_name":"Christian","full_name":"Knapp, Christian"},{"last_name":"Xie","full_name":"Xie, Yufei","first_name":"Yufei"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack"},{"full_name":"Weghuber, Julian","first_name":"Julian","last_name":"Weghuber"},{"last_name":"Schaaf","first_name":"Marcel","full_name":"Schaaf, Marcel"},{"first_name":"Romain","full_name":"Quidant, Romain","last_name":"Quidant"},{"last_name":"Kiermaier","orcid":"0000-0001-6165-5738","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","full_name":"Kiermaier, Eva"},{"last_name":"Ortega Arroyo","full_name":"Ortega Arroyo, Jaime","first_name":"Jaime"},{"orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena","full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Wieser","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"}],"oa":1,"citation":{"chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>. Elsevier, 2025.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2025). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell (2025).","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2025. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2025. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>."},"article_processing_charge":"Yes (in subscription journal)","ddc":["570"],"day":"04","publication_status":"epub_ahead","OA_type":"hybrid","date_published":"2025-11-04T00:00:00Z","has_accepted_license":"1"},{"ddc":["539","570"],"publication_status":"draft","day":"25","article_processing_charge":"No","citation":{"ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening.” bioRxiv.","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” bioRxiv, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, (n.d.).","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","mla":"Dunajova, Zuzana, et al. <i>Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening</i>. bioRxiv, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>."},"corr_author":"1","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"name":"Motile active matter models of migrating cells and chiral filaments","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360"}],"date_published":"2025-09-25T00:00:00Z","has_accepted_license":"1","date_created":"2026-03-11T08:40:06Z","publisher":"bioRxiv","language":[{"iso":"eng"}],"oa_version":"Preprint","related_material":{"record":[{"relation":"dissertation_contains","id":"21423","status":"public"},{"id":"21439","status":"public","relation":"research_data"}]},"OA_place":"repository","date_updated":"2026-06-10T09:41:11Z","main_file_link":[{"url":"https://doi.org/10.1101/2025.05.20.655037","open_access":"1"}],"status":"public","author":[{"id":"4B39F286-F248-11E8-B48F-1D18A9856A87","first_name":"Zuzana","full_name":"Dunajova, Zuzana","last_name":"Dunajova"},{"last_name":"Tasciyan","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","full_name":"Majek, Juraj","first_name":"Juraj","last_name":"Majek"},{"orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack"},{"last_name":"Sahai","first_name":"Erik","full_name":"Sahai, Erik"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","last_name":"Hannezo"}],"oa":1,"year":"2025","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"type":"preprint","month":"09","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"doi":"10.1101/2025.05.20.655037","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"_id":"21427"},{"acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","pmid":1,"abstract":[{"lang":"eng","text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues."}],"title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2025","external_id":{"pmid":["39603235"],"isi":["001434279000001"]},"type":"journal_article","month":"02","_id":"18807","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"file_date_updated":"2025-04-16T10:54:07Z","scopus_import":"1","issue":"4","doi":"10.1016/j.devcel.2024.10.024","publication_identifier":{"issn":["1534-5807"]},"has_accepted_license":"1","OA_type":"hybrid","volume":60,"publication_status":"published","ddc":["570"],"day":"24","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>, vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>.","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. 2025;60(4):567-580. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>.","apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>","ieee":"S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>, vol. 60, no. 4. Elsevier, pp. 567–580, 2025."},"project":[{"name":"Mechanisms of tissue size regulation in spinal cord development","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F7802"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011"}],"date_published":"2025-02-24T00:00:00Z","corr_author":"1","intvolume":"        60","quality_controlled":"1","OA_place":"publisher","date_updated":"2026-06-23T22:30:48Z","oa":1,"author":[{"first_name":"Stefanie","full_name":"Rus, Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","last_name":"Rus","orcid":"0000-0001-8703-1093"},{"id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David","full_name":"Brückner, David","last_name":"Brückner","orcid":"0000-0001-7205-2975"},{"last_name":"Minchington","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","full_name":"Minchington, Thomas","first_name":"Thomas"},{"last_name":"Greunz","first_name":"Martina","full_name":"Greunz, Martina","id":"48A59534-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kicheva","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","first_name":"Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87"}],"file":[{"access_level":"open_access","date_created":"2025-04-16T10:54:07Z","file_name":"2025_DevelopmentalCell_Lehr.pdf","checksum":"bb58db4a908a1f4aabe4004706154541","file_id":"19584","creator":"dernst","relation":"main_file","date_updated":"2025-04-16T10:54:07Z","success":1,"content_type":"application/pdf","file_size":6994499}],"isi":1,"status":"public","language":[{"iso":"eng"}],"publication":"Developmental Cell","date_created":"2025-01-09T11:25:47Z","publisher":"Elsevier","article_type":"original","page":"567-580","related_material":{"record":[{"id":"19763","status":"public","relation":"dissertation_contains"}]},"oa_version":"Published Version"},{"has_accepted_license":"1","volume":34,"ddc":["570"],"day":"08","publication_status":"published","citation":{"short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>.","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024."},"article_processing_charge":"Yes (via OA deal)","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742573"}],"date_published":"2024-01-08T00:00:00Z","corr_author":"1","intvolume":"        34","quality_controlled":"1","date_updated":"2025-09-04T11:39:10Z","author":[{"first_name":"Feyza N","full_name":"Arslan, Feyza N","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","last_name":"Arslan","orcid":"0000-0001-5809-9566"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin"},{"id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin","first_name":"Martin","last_name":"Loose","orcid":"0000-0001-7309-9724"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"oa":1,"isi":1,"file":[{"file_size":5183861,"content_type":"application/pdf","success":1,"date_updated":"2024-01-16T10:53:31Z","relation":"main_file","creator":"dernst","file_id":"14813","checksum":"51220b76d72a614208f84bdbfbaf9b72","file_name":"2024_CurrentBiology_Arslan.pdf","date_created":"2024-01-16T10:53:31Z","access_level":"open_access"}],"status":"public","language":[{"iso":"eng"}],"publication":"Current Biology","publisher":"Elsevier","date_created":"2024-01-14T23:00:56Z","article_type":"original","page":"171-182.e8","oa_version":"Published Version","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","pmid":1,"abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","external_id":{"isi":["001154500400001"],"pmid":["38134934"]},"type":"journal_article","month":"01","_id":"14795","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"file_date_updated":"2024-01-16T10:53:31Z","ec_funded":1,"scopus_import":"1","issue":"1","doi":"10.1016/j.cub.2023.11.067","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]}},{"corr_author":"1","project":[{"_id":"2646861A-B435-11E9-9278-68D0E5697425","name":"Control of embryonic cleavage pattern","call_identifier":"FWF","grant_number":"I03601"}],"date_published":"2024-02-01T00:00:00Z","citation":{"apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321."},"article_processing_charge":"Yes (in subscription journal)","day":"01","ddc":["530"],"publication_status":"published","volume":20,"has_accepted_license":"1","oa_version":"Published Version","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","description":"News on ISTA Website"}]},"page":"310-321","article_type":"original","date_created":"2024-01-21T23:00:57Z","publisher":"Springer Nature","publication":"Nature Physics","language":[{"iso":"eng"}],"status":"public","isi":1,"file":[{"content_type":"application/pdf","file_size":9897883,"creator":"dernst","relation":"main_file","success":1,"date_updated":"2024-07-16T12:12:43Z","checksum":"7891ebe7c900ae47469ab127031dd1ec","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","file_id":"17267","access_level":"open_access","date_created":"2024-07-16T12:12:43Z"}],"author":[{"orcid":"0000-0002-5223-3346","last_name":"Caballero Mancebo","first_name":"Silvia","full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Shinde, Rushikesh","first_name":"Rushikesh","last_name":"Shinde"},{"orcid":"0000-0002-8176-4824","last_name":"Bolger-Munro","first_name":"Madison","full_name":"Bolger-Munro, Madison","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E"},{"orcid":"0000-0002-3415-4628","last_name":"Peruzzo","first_name":"Matilda","full_name":"Peruzzo, Matilda","id":"3F920B30-F248-11E8-B48F-1D18A9856A87"},{"id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory","full_name":"Szep, Gregory","last_name":"Szep"},{"last_name":"Steccari","first_name":"Irene","full_name":"Steccari, Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E"},{"last_name":"Labrousse Arias","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","first_name":"David","full_name":"Labrousse Arias, David"},{"orcid":"0000-0002-9438-4783","last_name":"Zheden","first_name":"Vanessa","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack"},{"first_name":"Andrew","full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones"},{"last_name":"Voituriez","full_name":"Voituriez, Raphaël","first_name":"Raphaël"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"oa":1,"date_updated":"2025-09-04T11:48:28Z","quality_controlled":"1","intvolume":"        20","month":"02","type":"journal_article","external_id":{"pmid":["38370025"],"isi":["001138880800005"]},"year":"2024","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}],"pmid":1,"acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","doi":"10.1038/s41567-023-02302-1","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"scopus_import":"1","file_date_updated":"2024-07-16T12:12:43Z","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"_id":"14846"},{"_id":"15018","department":[{"_id":"GeKa"},{"_id":"NanoFab"}],"keyword":["Mechanical Engineering","Mechanics of Materials","Condensed Matter Physics","General Materials Science"],"file_date_updated":"2024-07-22T11:56:08Z","scopus_import":"1","issue":"5","doi":"10.1016/j.mssp.2024.108231","publication_identifier":{"issn":["1369-8001"]},"article_number":"108231","acknowledgement":"The Ge project received funding from the European Union's Horizon Europe programme under the Grant Agreement 101069515 – IGNITE. Siltronic AG is acknowledged for providing the SRB wafers. This work was supported by Imec's Industrial Affiliation Program on Quantum Computing.","abstract":[{"lang":"eng","text":"The epitaxial growth of a strained Ge layer, which is a promising candidate for the channel material of a hole spin qubit, has been demonstrated on 300 mm Si wafers using commercially available Si0.3Ge0.7 strain relaxed buffer (SRB) layers. The assessment of the layer and the interface qualities for a buried strained Ge layer embedded in Si0.3Ge0.7 layers is reported. The XRD reciprocal space mapping confirmed that the reduction of the growth temperature enables the 2-dimensional growth of the Ge layer fully strained with respect to the Si0.3Ge0.7. Nevertheless, dislocations at the top and/or bottom interface of the Ge layer were observed by means of electron channeling contrast imaging, suggesting the importance of the careful dislocation assessment. The interface abruptness does not depend on the selection of the precursor gases, but it is strongly influenced by the growth temperature which affects the coverage of the surface H-passivation. The mobility of 2.7 × 105 cm2/Vs is promising, while the low percolation density of 3 × 1010 /cm2 measured with a Hall-bar device at 7 K illustrates the high quality of the heterostructure thanks to the high Si0.3Ge0.7 SRB quality."}],"title":"Compressively strained epitaxial Ge layers for quantum computing applications","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","external_id":{"isi":["001188520000001"]},"type":"journal_article","month":"05","quality_controlled":"1","intvolume":"       174","OA_place":"publisher","date_updated":"2025-04-14T08:01:27Z","oa":1,"author":[{"full_name":"Shimura, Yosuke","first_name":"Yosuke","last_name":"Shimura"},{"full_name":"Godfrin, Clement","first_name":"Clement","last_name":"Godfrin"},{"full_name":"Hikavyy, Andriy","first_name":"Andriy","last_name":"Hikavyy"},{"last_name":"Li","full_name":"Li, Roy","first_name":"Roy"},{"last_name":"Aguilera Servin","orcid":"0000-0002-2862-8372","first_name":"Juan L","full_name":"Aguilera Servin, Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Katsaros","orcid":"0000-0001-8342-202X","id":"38DB5788-F248-11E8-B48F-1D18A9856A87","first_name":"Georgios","full_name":"Katsaros, Georgios"},{"first_name":"Paola","full_name":"Favia, Paola","last_name":"Favia"},{"first_name":"Han","full_name":"Han, Han","last_name":"Han"},{"full_name":"Wan, Danny","first_name":"Danny","last_name":"Wan"},{"first_name":"Kristiaan","full_name":"de Greve, Kristiaan","last_name":"de Greve"},{"first_name":"Roger","full_name":"Loo, Roger","last_name":"Loo"}],"isi":1,"file":[{"file_name":"2024_MaterialsScience_Shimura.pdf","checksum":"62e8e9ae960387a3dca32ec7f5e413ab","file_id":"17312","access_level":"open_access","date_created":"2024-07-22T11:56:08Z","content_type":"application/pdf","file_size":4220165,"creator":"dernst","relation":"main_file","date_updated":"2024-07-22T11:56:08Z","success":1}],"status":"public","language":[{"iso":"eng"}],"publication":"Materials Science in Semiconductor Processing","publisher":"Elsevier","date_created":"2024-02-22T14:10:40Z","article_type":"original","oa_version":"Published Version","has_accepted_license":"1","OA_type":"hybrid","volume":174,"day":"20","ddc":["530"],"publication_status":"published","citation":{"ista":"Shimura Y, Godfrin C, Hikavyy A, Li R, Aguilera Servin JL, Katsaros G, Favia P, Han H, Wan D, de Greve K, Loo R. 2024. Compressively strained epitaxial Ge layers for quantum computing applications. Materials Science in Semiconductor Processing. 174(5), 108231.","ama":"Shimura Y, Godfrin C, Hikavyy A, et al. Compressively strained epitaxial Ge layers for quantum computing applications. <i>Materials Science in Semiconductor Processing</i>. 2024;174(5). doi:<a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">10.1016/j.mssp.2024.108231</a>","mla":"Shimura, Yosuke, et al. “Compressively Strained Epitaxial Ge Layers for Quantum Computing Applications.” <i>Materials Science in Semiconductor Processing</i>, vol. 174, no. 5, 108231, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">10.1016/j.mssp.2024.108231</a>.","short":"Y. Shimura, C. Godfrin, A. Hikavyy, R. Li, J.L. Aguilera Servin, G. Katsaros, P. Favia, H. Han, D. Wan, K. de Greve, R. Loo, Materials Science in Semiconductor Processing 174 (2024).","chicago":"Shimura, Yosuke, Clement Godfrin, Andriy Hikavyy, Roy Li, Juan L Aguilera Servin, Georgios Katsaros, Paola Favia, et al. “Compressively Strained Epitaxial Ge Layers for Quantum Computing Applications.” <i>Materials Science in Semiconductor Processing</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">https://doi.org/10.1016/j.mssp.2024.108231</a>.","apa":"Shimura, Y., Godfrin, C., Hikavyy, A., Li, R., Aguilera Servin, J. L., Katsaros, G., … Loo, R. (2024). Compressively strained epitaxial Ge layers for quantum computing applications. <i>Materials Science in Semiconductor Processing</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">https://doi.org/10.1016/j.mssp.2024.108231</a>","ieee":"Y. Shimura <i>et al.</i>, “Compressively strained epitaxial Ge layers for quantum computing applications,” <i>Materials Science in Semiconductor Processing</i>, vol. 174, no. 5. Elsevier, 2024."},"article_processing_charge":"Yes (in subscription journal)","project":[{"_id":"34c0acea-11ca-11ed-8bc3-8775e10fd452","name":"Integrated Germanium Quantum Technology","grant_number":"101069515"}],"date_published":"2024-05-20T00:00:00Z"},{"status":"public","isi":1,"file":[{"content_type":"application/pdf","file_size":2537502,"relation":"main_file","creator":"dernst","success":1,"date_updated":"2024-08-05T08:19:58Z","checksum":"6141d05cd68d540a7446dce9490975db","file_name":"2024_JourApplPhysics_Lenton.pdf","file_id":"17386","access_level":"open_access","date_created":"2024-08-05T08:19:58Z"}],"oa":1,"author":[{"id":"a550210f-223c-11ec-8182-e2d45e817efb","first_name":"Isaac C","full_name":"Lenton, Isaac C","last_name":"Lenton","orcid":"0000-0002-5010-6984"},{"last_name":"Pertl","orcid":"0000-0003-0463-5794","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","full_name":"Pertl, Felix","first_name":"Felix"},{"last_name":"Shafeek","orcid":"0000-0001-7180-6050","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87","first_name":"Lubuna B","full_name":"Shafeek, Lubuna B"},{"id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","full_name":"Waitukaitis, Scott R","first_name":"Scott R","last_name":"Waitukaitis","orcid":"0000-0002-2299-3176"}],"date_updated":"2025-09-08T08:47:42Z","intvolume":"       136","quality_controlled":"1","oa_version":"Published Version","article_type":"original","publisher":"AIP Publishing","date_created":"2024-08-04T22:01:21Z","publication":"Journal of Applied Physics","language":[{"iso":"eng"}],"has_accepted_license":"1","corr_author":"1","date_published":"2024-07-28T00:00:00Z","project":[{"call_identifier":"H2020","grant_number":"949120","name":"Tribocharge: a multi-scale approach to an enduring problem in physics","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa"}],"citation":{"short":"I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Journal of Applied Physics 136 (2024).","ama":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging. <i>Journal of Applied Physics</i>. 2024;136(4). doi:<a href=\"https://doi.org/10.1063/5.0215151\">10.1063/5.0215151</a>","ista":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2024. Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging. Journal of Applied Physics. 136(4), 045305.","mla":"Lenton, Isaac C., et al. “Beyond the Blur: Using Experimentally Determined Point Spread Functions to Improve Scanning Kelvin Probe Imaging.” <i>Journal of Applied Physics</i>, vol. 136, no. 4, 045305, AIP Publishing, 2024, doi:<a href=\"https://doi.org/10.1063/5.0215151\">10.1063/5.0215151</a>.","ieee":"I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging,” <i>Journal of Applied Physics</i>, vol. 136, no. 4. AIP Publishing, 2024.","chicago":"Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis. “Beyond the Blur: Using Experimentally Determined Point Spread Functions to Improve Scanning Kelvin Probe Imaging.” <i>Journal of Applied Physics</i>. AIP Publishing, 2024. <a href=\"https://doi.org/10.1063/5.0215151\">https://doi.org/10.1063/5.0215151</a>.","apa":"Lenton, I. C., Pertl, F., Shafeek, L. B., &#38; Waitukaitis, S. R. (2024). Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging. <i>Journal of Applied Physics</i>. AIP Publishing. <a href=\"https://doi.org/10.1063/5.0215151\">https://doi.org/10.1063/5.0215151</a>"},"article_processing_charge":"No","day":"28","ddc":["530"],"publication_status":"published","volume":136,"department":[{"_id":"ScWa"},{"_id":"NanoFab"}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"_id":"17373","publication_identifier":{"eissn":["1089-7550"],"issn":["0021-8979"]},"doi":"10.1063/5.0215151","issue":"4","scopus_import":"1","ec_funded":1,"file_date_updated":"2024-08-05T08:19:58Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging","abstract":[{"lang":"eng","text":"Scanning Kelvin probe microscopy (SKPM) is a powerful technique for investigating the electrostatic properties of material surfaces, enabling the imaging of variations in work function, topology, surface charge density, or combinations thereof. Regardless of the underlying signal source, SKPM results in a voltage image, which is spatially distorted due to the finite size of the probe, long-range electrostatic interactions, mechanical and electrical noise, and the finite response time of the electronics. In order to recover the underlying signal, it is necessary to deconvolve the measurement with an appropriate point spread function (PSF) that accounts the aforementioned distortions, but determining this PSF is difficult. Here, we describe how such PSFs can be determined experimentally and show how they can be used to recover the underlying information of interest. We first consider the physical principles that enable SKPM and discuss how these affect the system PSF. We then show how one can experimentally measure PSFs by looking at well-defined features, and that these compare well to simulated PSFs, provided scans are performed extremely slowly and carefully. Next, we work at realistic scan speeds and show that the idealized PSFs fail to capture temporal distortions in the scan direction. While simulating PSFs for these situations would be quite challenging, we show that measuring PSFs with similar scan conditions works well. Our approach clarifies the basic principles and inherent challenges to SKPM measurements and gives practical methods to improve results."}],"acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 949120). This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan Carlos Sobarzo for letting us use their equipment. The authors wish to thank the contributions of the whole Waitukaitis Group for useful discussions and feedback.","article_number":"045305","month":"07","type":"journal_article","external_id":{"isi":["001281681100003"]},"year":"2024","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"}},{"department":[{"_id":"NanoFab"}],"_id":"17479","file_date_updated":"2025-01-09T14:01:06Z","doi":"10.1021/acsphotonics.4c00485","issue":"9","publication_identifier":{"eissn":["2330-4022"]},"scopus_import":"1","acknowledgement":"Funding Sources ─ A.I.F.T.-M. and G.Á.-P. acknowledge support through the Severo Ochoa program from the Government of the Principality of Asturias (references PA-21-PF-BP20-117 and PA20-PF-BP19-053, respectively). A.B.K. and J.T.-G. acknowledge support from the Swiss National Science Foundation (grant # 200020_201096). J.M.-S. acknowledges financial support from the Ramón y Cajal Program of the Government of Spain and FSE (RYC2018-026196-I), the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-110308GA-I00/AEI/10.13039/501100011033) and project PCI2022-132953 funded by MCIN/AEI/10.13039/501100011033 and the EU “NextGenerationEU”/PRTR”. P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA and the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-111156GB-I00). A.Y.N. acknowledges the Spanish Ministry of Science and Innovation (grant PID2020-115221GB-C42) and the Basque Department of Education (grant PIBA-2023-1-0007). M.V. and J.I.M. acknowledge support by Spanish MCIN/AEI/10.13039/501100011033/FEDER, UE under grant PID2022-136784NB and by Asturias FICYT under grant AYUD/2021/51185 with the support of FEDER funds. I.E. acknowledges funding from the Spanish Ministry of Science and Innovation (Grant No. PID2022-142861NA-I00) and the Department of Education, Universities, and Research of the Eusko Jaurlaritza and the University of the Basque Country UPV/EHU (Grant No. IT1527-22). J. Duan acknowledges the support from the Beijing Natural Science Foundation (Grant No. Z240005), and National Natural Science Foundation of China.","title":"Unveiling the mechanism of phonon-polariton damping in α‑MoO3","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"abstract":[{"lang":"eng","text":"Phonon polaritons (PhPs), light coupled to lattice vibrations, in the highly anisotropic polar layered material molybdenum trioxide (α-MoO3) are currently the focus of intense research efforts due to their extreme subwavelength field confinement, directional propagation, and unprecedented low losses. Nevertheless, prior research has primarily concentrated on exploiting the squeezing and steering capabilities of α-MoO3 PhPs, without inquiring much into the dominant microscopic mechanism that determines their long lifetimes, which is key for their implementation in nanophotonic applications. This study delves into the fundamental processes that govern PhP damping in α-MoO3 by combining ab initio calculations with scattering-type scanning near-field optical microscopy (s-SNOM) and Fourier transform infrared (FTIR) spectroscopy measurements across a broad temperature range (8–300 K). The remarkable agreement between our theoretical predictions and experimental observations allows us to identify third-order anharmonic phonon–phonon scattering as the main damping mechanism of α-MoO3 PhPs. These findings shed light on the fundamental limits of low-loss PhPs, which is a crucial factor for assessing their implementation into nanophotonic devices."}],"year":"2024","external_id":{"arxiv":["2408.09811"],"isi":["001298164600001"],"pmid":["39310295"]},"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","month":"09","OA_place":"publisher","date_updated":"2025-09-08T09:05:01Z","quality_controlled":"1","intvolume":"        11","status":"public","author":[{"full_name":"Taboada-Gutiérrez, Javier","first_name":"Javier","last_name":"Taboada-Gutiérrez"},{"last_name":"Zhou","first_name":"Yixi","full_name":"Zhou, Yixi"},{"last_name":"Tresguerres-Mata","full_name":"Tresguerres-Mata, Ana I.F.","first_name":"Ana I.F."},{"last_name":"Lanza","first_name":"Christian","full_name":"Lanza, Christian"},{"first_name":"Abel","full_name":"Martínez-Suárez, Abel","last_name":"Martínez-Suárez"},{"last_name":"Álvarez-Pérez","first_name":"Gonzalo","full_name":"Álvarez-Pérez, Gonzalo"},{"full_name":"Duan, Jiahua","first_name":"Jiahua","last_name":"Duan"},{"full_name":"Martín, José Ignacio","first_name":"José Ignacio","last_name":"Martín"},{"last_name":"Vélez","full_name":"Vélez, María","first_name":"María"},{"orcid":"0000-0002-7370-5357","last_name":"Prieto Gonzalez","full_name":"Prieto Gonzalez, Ivan","first_name":"Ivan","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Adrien","full_name":"Bercher, Adrien","last_name":"Bercher"},{"last_name":"Teyssier","first_name":"Jérémie","full_name":"Teyssier, Jérémie"},{"first_name":"Ion","full_name":"Errea, Ion","last_name":"Errea"},{"last_name":"Nikitin","first_name":"Alexey Y.","full_name":"Nikitin, Alexey Y."},{"full_name":"Martín-Sánchez, Javier","first_name":"Javier","last_name":"Martín-Sánchez"},{"first_name":"Alexey B.","full_name":"Kuzmenko, Alexey B.","last_name":"Kuzmenko"},{"last_name":"Alonso-González","full_name":"Alonso-González, Pablo","first_name":"Pablo"}],"oa":1,"file":[{"checksum":"bd7e6a138c406e93eaf0a6268fc42bfe","file_name":"2024_ACSPhotonics_TaboadaGutierrez_.pdf","file_id":"18819","access_level":"open_access","date_created":"2025-01-09T14:01:06Z","file_size":2664512,"content_type":"application/pdf","relation":"main_file","creator":"dernst","success":1,"date_updated":"2025-01-09T14:01:06Z"}],"isi":1,"publisher":"American Chemical Society","date_created":"2024-09-01T22:01:09Z","language":[{"iso":"eng"}],"publication":"ACS Photonics","page":"3570-3577","arxiv":1,"oa_version":"Published Version","article_type":"original","has_accepted_license":"1","publication_status":"published","day":"01","ddc":["530"],"citation":{"short":"J. Taboada-Gutiérrez, Y. Zhou, A.I.F. Tresguerres-Mata, C. Lanza, A. Martínez-Suárez, G. Álvarez-Pérez, J. Duan, J.I. Martín, M. Vélez, I. Prieto Gonzalez, A. Bercher, J. Teyssier, I. Errea, A.Y. Nikitin, J. Martín-Sánchez, A.B. Kuzmenko, P. Alonso-González, ACS Photonics 11 (2024) 3570–3577.","mla":"Taboada-Gutiérrez, Javier, et al. “Unveiling the Mechanism of Phonon-Polariton Damping in Α‑MoO3.” <i>ACS Photonics</i>, vol. 11, no. 9, American Chemical Society, 2024, pp. 3570–77, doi:<a href=\"https://doi.org/10.1021/acsphotonics.4c00485\">10.1021/acsphotonics.4c00485</a>.","ista":"Taboada-Gutiérrez J, Zhou Y, Tresguerres-Mata AIF, Lanza C, Martínez-Suárez A, Álvarez-Pérez G, Duan J, Martín JI, Vélez M, Prieto Gonzalez I, Bercher A, Teyssier J, Errea I, Nikitin AY, Martín-Sánchez J, Kuzmenko AB, Alonso-González P. 2024. Unveiling the mechanism of phonon-polariton damping in α‑MoO3. ACS Photonics. 11(9), 3570–3577.","ama":"Taboada-Gutiérrez J, Zhou Y, Tresguerres-Mata AIF, et al. Unveiling the mechanism of phonon-polariton damping in α‑MoO3. <i>ACS Photonics</i>. 2024;11(9):3570-3577. doi:<a href=\"https://doi.org/10.1021/acsphotonics.4c00485\">10.1021/acsphotonics.4c00485</a>","apa":"Taboada-Gutiérrez, J., Zhou, Y., Tresguerres-Mata, A. I. F., Lanza, C., Martínez-Suárez, A., Álvarez-Pérez, G., … Alonso-González, P. (2024). Unveiling the mechanism of phonon-polariton damping in α‑MoO3. <i>ACS Photonics</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsphotonics.4c00485\">https://doi.org/10.1021/acsphotonics.4c00485</a>","chicago":"Taboada-Gutiérrez, Javier, Yixi Zhou, Ana I.F. Tresguerres-Mata, Christian Lanza, Abel Martínez-Suárez, Gonzalo Álvarez-Pérez, Jiahua Duan, et al. “Unveiling the Mechanism of Phonon-Polariton Damping in Α‑MoO3.” <i>ACS Photonics</i>. American Chemical Society, 2024. <a href=\"https://doi.org/10.1021/acsphotonics.4c00485\">https://doi.org/10.1021/acsphotonics.4c00485</a>.","ieee":"J. Taboada-Gutiérrez <i>et al.</i>, “Unveiling the mechanism of phonon-polariton damping in α‑MoO3,” <i>ACS Photonics</i>, vol. 11, no. 9. American Chemical Society, pp. 3570–3577, 2024."},"article_processing_charge":"No","OA_type":"hybrid","volume":11,"date_published":"2024-09-01T00:00:00Z"},{"department":[{"_id":"AnKi"},{"_id":"NanoFab"}],"_id":"18601","acknowledged_ssus":[{"_id":"NanoFab"}],"file_date_updated":"2024-12-03T10:53:23Z","issue":"4","doi":"10.1016/j.xpro.2024.103187","publication_identifier":{"eissn":["2666-1667"]},"scopus_import":"1","acknowledgement":"We thank the nanofabrication facility at ISTA for technical assistance. Work in the A.K. lab is supported by ISTA, the European Research Council under Horizon Europe (grant 101044579), and the Austrian Science Fund (FWF) (grant https://doi.org/10.55776/F78). S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011.","article_number":"103187","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Protocol for fabricating elastomeric stencils for patterned stem cell differentiation","abstract":[{"text":"Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithography to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils with through holes. We then provide instructions for culturing cells on stencils and, finally, removing stencils to allow colony growth and cell migration. This approach yields reproducible two-dimensional organoids tailored for quantitative studies of growth and pattern formation.\r\nFor complete details on the use and execution of this protocol, please refer to Lehr et al.1","lang":"eng"}],"DOAJ_listed":"1","pmid":1,"external_id":{"pmid":["39602310"]},"year":"2024","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"month":"12","type":"journal_article","date_updated":"2026-06-23T22:30:48Z","OA_place":"publisher","intvolume":"         5","quality_controlled":"1","status":"public","APC_amount":"804 EUR","file":[{"date_updated":"2024-12-03T10:53:23Z","success":1,"relation":"main_file","creator":"dernst","file_size":4989169,"content_type":"application/pdf","date_created":"2024-12-03T10:53:23Z","access_level":"open_access","file_id":"18610","checksum":"0c61a6f9978608a103865905e06f4581","file_name":"2024_STARProtoc_Lehr.pdf"}],"author":[{"last_name":"Rus","orcid":"0000-0001-8703-1093","full_name":"Rus, Stefanie","first_name":"Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"3331f5ae-e896-11ec-af79-eeb79769bcb7","first_name":"Monika Aleksandra","full_name":"Kulig, Monika Aleksandra","last_name":"Kulig"},{"first_name":"Thomas","full_name":"Minchington, Thomas","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","last_name":"Minchington"},{"id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","full_name":"Kicheva, Anna","first_name":"Anna","last_name":"Kicheva","orcid":"0000-0003-4509-4998"}],"oa":1,"date_created":"2024-12-01T23:01:53Z","publisher":"Elsevier","publication":"STAR Protocols","language":[{"iso":"eng"}],"related_material":{"record":[{"id":"19763","status":"public","relation":"dissertation_contains"}]},"oa_version":"Published Version","article_type":"original","has_accepted_license":"1","citation":{"chicago":"Rus, Stefanie, Jack Merrin, Monika Aleksandra Kulig, Thomas Minchington, and Anna Kicheva. “Protocol for Fabricating Elastomeric Stencils for Patterned Stem Cell Differentiation.” <i>STAR Protocols</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.xpro.2024.103187\">https://doi.org/10.1016/j.xpro.2024.103187</a>.","ieee":"S. Rus, J. Merrin, M. A. Kulig, T. Minchington, and A. Kicheva, “Protocol for fabricating elastomeric stencils for patterned stem cell differentiation,” <i>STAR Protocols</i>, vol. 5, no. 4. Elsevier, 2024.","apa":"Rus, S., Merrin, J., Kulig, M. A., Minchington, T., &#38; Kicheva, A. (2024). Protocol for fabricating elastomeric stencils for patterned stem cell differentiation. <i>STAR Protocols</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xpro.2024.103187\">https://doi.org/10.1016/j.xpro.2024.103187</a>","ista":"Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. 2024. Protocol for fabricating elastomeric stencils for patterned stem cell differentiation. STAR Protocols. 5(4), 103187.","ama":"Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. Protocol for fabricating elastomeric stencils for patterned stem cell differentiation. <i>STAR Protocols</i>. 2024;5(4). doi:<a href=\"https://doi.org/10.1016/j.xpro.2024.103187\">10.1016/j.xpro.2024.103187</a>","mla":"Rus, Stefanie, et al. “Protocol for Fabricating Elastomeric Stencils for Patterned Stem Cell Differentiation.” <i>STAR Protocols</i>, vol. 5, no. 4, 103187, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.xpro.2024.103187\">10.1016/j.xpro.2024.103187</a>.","short":"S. Rus, J. Merrin, M.A. Kulig, T. Minchington, A. Kicheva, STAR Protocols 5 (2024)."},"article_processing_charge":"Yes","day":"20","publication_status":"published","ddc":["570"],"volume":5,"OA_type":"gold","corr_author":"1","project":[{"grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011"}],"date_published":"2024-12-20T00:00:00Z"},{"department":[{"_id":"MiSi"},{"_id":"NanoFab"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"},{"_id":"M-Shop"}],"_id":"13052","ec_funded":1,"doi":"10.1007/978-1-0716-3135-5_9","publication_identifier":{"eissn":["1940-6029"],"eisbn":["9781071631355"],"isbn":["9781071631348"],"issn":["1064-3745"]},"scopus_import":"1","acknowledgement":"A.L. was funded by an Erwin Schrödinger postdoctoral fellowship of the Austrian Science Fund (FWF, project number: J4542-B) and is an EMBO non-stipendiary postdoctoral fellow. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. We thank the Imaging & Optics facility, the Nanofabrication facility, and the Miba Machine Shop of ISTA for their excellent support.","title":"En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"editor":[{"last_name":"Baldari","first_name":"Cosima","full_name":"Baldari, Cosima"},{"first_name":"Michael","full_name":"Dustin, Michael","last_name":"Dustin"}],"abstract":[{"text":"Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell contacts along the vertical imaging plane. This requires optical sectioning that often results in unsatisfactory resolution in time and space. Here, we present a workflow where DCs and T cells are confined between a layer of glass and polydimethylsiloxane (PDMS) that orients the cells along one, horizontal imaging plane, allowing for fast en-face-imaging of the DC-T cell IS.","lang":"eng"}],"year":"2023","external_id":{"pmid":["37106180"]},"alternative_title":["Methods in Molecular Biology"],"type":"book_chapter","month":"04","date_updated":"2025-04-14T07:42:07Z","intvolume":"      2654","quality_controlled":"1","status":"public","author":[{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F","full_name":"Leithner, Alexander F","orcid":"0000-0002-1073-744X","last_name":"Leithner"},{"first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"series_title":"MIMB","publisher":"Springer Nature","date_created":"2023-05-22T08:41:48Z","place":"New York, NY","language":[{"iso":"eng"}],"publication":"The Immune Synapse","page":"137-147","oa_version":"None","publication_status":"published","day":"28","citation":{"ieee":"A. F. Leithner, J. Merrin, and M. K. Sixt, “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses,” in <i>The Immune Synapse</i>, vol. 2654, C. Baldari and M. Dustin, Eds. New York, NY: Springer Nature, 2023, pp. 137–147.","apa":"Leithner, A. F., Merrin, J., &#38; Sixt, M. K. (2023). En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In C. Baldari &#38; M. Dustin (Eds.), <i>The Immune Synapse</i> (Vol. 2654, pp. 137–147). New York, NY: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">https://doi.org/10.1007/978-1-0716-3135-5_9</a>","chicago":"Leithner, Alexander F, Jack Merrin, and Michael K Sixt. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” In <i>The Immune Synapse</i>, edited by Cosima Baldari and Michael Dustin, 2654:137–47. MIMB. New York, NY: Springer Nature, 2023. <a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">https://doi.org/10.1007/978-1-0716-3135-5_9</a>.","short":"A.F. Leithner, J. Merrin, M.K. Sixt, in:, C. Baldari, M. Dustin (Eds.), The Immune Synapse, Springer Nature, New York, NY, 2023, pp. 137–147.","mla":"Leithner, Alexander F., et al. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” <i>The Immune Synapse</i>, edited by Cosima Baldari and Michael Dustin, vol. 2654, Springer Nature, 2023, pp. 137–47, doi:<a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">10.1007/978-1-0716-3135-5_9</a>.","ista":"Leithner AF, Merrin J, Sixt MK. 2023.En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: The Immune Synapse. Methods in Molecular Biology, vol. 2654, 137–147.","ama":"Leithner AF, Merrin J, Sixt MK. En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: Baldari C, Dustin M, eds. <i>The Immune Synapse</i>. Vol 2654. MIMB. New York, NY: Springer Nature; 2023:137-147. doi:<a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">10.1007/978-1-0716-3135-5_9</a>"},"article_processing_charge":"No","volume":2654,"project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","call_identifier":"H2020","grant_number":"724373"}],"date_published":"2023-04-28T00:00:00Z"},{"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"year":"2023","external_id":{"pmid":["37987147"],"isi":["001120971800001"]},"type":"journal_article","month":"11","article_number":"e114557","acknowledgement":"We thank Christoph Mayr and Bingzhi Wang for initial experiments on amoeboid nucleokinesis, Ana-Maria Lennon-Duménil and Aline Yatim for bone marrow from MyoIIA-Flox*CD11c-Cre mice, Michael Sixt and Aglaja Kopf for EMTB-mCherry, EB3-mCherry, Lifeact-GFP, Lfc knockout, and Myh9-GFP expressing HoxB8 cells, Malte Benjamin Braun, Mauricio Ruiz, and Madeleine T. Schmitt for critical reading of the manuscript, and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support. This study was supported by the Peter Hans Hofschneider Professorship of the foundation “Stiftung Experimentelle Biomedizin” (to JR), the LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (to JR), and the Deutsche Forschungsgemeinschaft (DFG; German Research Foundation; SFB914 project A12, to JR), and the CZI grant DAF2020-225401 (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (to RH; an advised fund of Silicon Valley Community Foundation (funder https://doi.org/10.13039/100014989)). Open Access funding enabled and organized by Projekt DEAL.","pmid":1,"abstract":[{"lang":"eng","text":"Motile cells moving in multicellular organisms encounter microenvironments of locally heterogeneous mechanochemical composition. Individual compositional parameters like chemotactic signals, adhesiveness, and pore sizes are well known to be sensed by motile cells, providing individual guidance cues for cellular pathfinding. However, motile cells encounter diverse mechanochemical signals at the same time, raising the question of how cells respond to locally diverse and potentially competing signals on their migration routes. Here, we reveal that motile amoeboid cells require nuclear repositioning, termed nucleokinesis, for adaptive pathfinding in heterogeneous mechanochemical microenvironments. Using mammalian immune cells and the amoeba<jats:italic>Dictyostelium discoideum</jats:italic>, we discover that frequent, rapid and long-distance nucleokinesis is a basic component of amoeboid pathfinding, enabling cells to reorientate quickly between locally competing cues. Amoeboid nucleokinesis comprises a two-step cell polarity switch and is driven by myosin II-forces, sliding the nucleus from a ‘losing’ to the ‘winning’ leading edge to re-adjust the nuclear to the cellular path. Impaired nucleokinesis distorts fast path adaptions and causes cellular arrest in the microenvironment. Our findings establish that nucleokinesis is required for amoeboid cell navigation. Given that motile single-cell amoebae, many immune cells, and some cancer cells utilize an amoeboid migration strategy, these results suggest that amoeboid nucleokinesis underlies cellular navigation during unicellular biology, immunity, and disease."}],"title":"Adaptive pathfinding by nucleokinesis during amoeboid migration","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2023-11-27T08:45:56Z","scopus_import":"1","doi":"10.15252/embj.2023114557","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"_id":"13342","department":[{"_id":"NanoFab"},{"_id":"Bio"}],"publication_status":"published","day":"21","ddc":["570"],"article_processing_charge":"Yes (via OA deal)","citation":{"chicago":"Kroll, Janina, Robert Hauschild, Arthur Kuznetcov, Kasia Stefanowski, Monika D. Hermann, Jack Merrin, Lubuna B Shafeek, Annette Müller-Taubenberger, and Jörg Renkawitz. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>. Embo Press, 2023. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>.","ieee":"J. Kroll <i>et al.</i>, “Adaptive pathfinding by nucleokinesis during amoeboid migration,” <i>EMBO Journal</i>. Embo Press, 2023.","apa":"Kroll, J., Hauschild, R., Kuznetcov, A., Stefanowski, K., Hermann, M. D., Merrin, J., … Renkawitz, J. (2023). Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>","mla":"Kroll, Janina, et al. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>, e114557, Embo Press, 2023, doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>.","ama":"Kroll J, Hauschild R, Kuznetcov A, et al. Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. 2023. doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>","ista":"Kroll J, Hauschild R, Kuznetcov A, Stefanowski K, Hermann MD, Merrin J, Shafeek LB, Müller-Taubenberger A, Renkawitz J. 2023. Adaptive pathfinding by nucleokinesis during amoeboid migration. EMBO Journal., e114557.","short":"J. Kroll, R. Hauschild, A. Kuznetcov, K. Stefanowski, M.D. Hermann, J. Merrin, L.B. Shafeek, A. Müller-Taubenberger, J. Renkawitz, EMBO Journal (2023)."},"date_published":"2023-11-21T00:00:00Z","has_accepted_license":"1","language":[{"iso":"eng"}],"publication":"EMBO Journal","publisher":"Embo Press","date_created":"2023-08-01T08:59:06Z","article_type":"original","oa_version":"Published Version","quality_controlled":"1","date_updated":"2025-09-09T12:44:04Z","author":[{"first_name":"Janina","full_name":"Kroll, Janina","last_name":"Kroll"},{"last_name":"Hauschild","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kuznetcov","first_name":"Arthur","full_name":"Kuznetcov, Arthur"},{"last_name":"Stefanowski","full_name":"Stefanowski, Kasia","first_name":"Kasia"},{"full_name":"Hermann, Monika D.","first_name":"Monika D.","last_name":"Hermann"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack"},{"last_name":"Shafeek","orcid":"0000-0001-7180-6050","full_name":"Shafeek, Lubuna B","first_name":"Lubuna B","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Annette","full_name":"Müller-Taubenberger, Annette","last_name":"Müller-Taubenberger"},{"full_name":"Renkawitz, Jörg","first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2856-3369","last_name":"Renkawitz"}],"oa":1,"file":[{"content_type":"application/pdf","file_size":4862497,"relation":"main_file","creator":"dernst","success":1,"date_updated":"2023-11-27T08:45:56Z","file_name":"2023_EmboJournal_Kroll.pdf","checksum":"6261d0041c7e8d284c39712c40079730","file_id":"14611","access_level":"open_access","date_created":"2023-11-27T08:45:56Z"}],"isi":1,"status":"public"},{"status":"public","file":[{"content_type":"application/pdf","file_size":2317272,"relation":"main_file","creator":"dernst","date_updated":"2023-09-25T08:32:37Z","success":1,"file_name":"2023_NatureComm_Riedl.pdf","checksum":"82d2d4ad736cc8493db8ce45cd313f7b","file_id":"14366","access_level":"open_access","date_created":"2023-09-25T08:32:37Z"}],"isi":1,"author":[{"orcid":"0000-0003-4844-6311","last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","full_name":"Riedl, Michael","first_name":"Michael"},{"last_name":"Mayer","full_name":"Mayer, Isabelle D","first_name":"Isabelle D","id":"61763940-15b2-11ec-abd3-cfaddfbc66b4"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K"},{"full_name":"Hof, Björn","first_name":"Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","orcid":"0000-0003-2057-2754"}],"oa":1,"date_updated":"2025-04-14T13:10:03Z","intvolume":"        14","quality_controlled":"1","oa_version":"Published Version","article_type":"original","publisher":"Springer Nature","date_created":"2023-09-24T22:01:10Z","publication":"Nature Communications","language":[{"iso":"eng"}],"has_accepted_license":"1","corr_author":"1","project":[{"call_identifier":"FP7","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"grant_number":"724373","call_identifier":"H2020","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"}],"date_published":"2023-09-13T00:00:00Z","article_processing_charge":"Yes","citation":{"apa":"Riedl, M., Mayer, I. D., Merrin, J., Sixt, M. K., &#38; Hof, B. (2023). Synchronization in collectively moving inanimate and living active matter. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-023-41432-1\">https://doi.org/10.1038/s41467-023-41432-1</a>","ieee":"M. Riedl, I. D. Mayer, J. Merrin, M. K. Sixt, and B. Hof, “Synchronization in collectively moving inanimate and living active matter,” <i>Nature Communications</i>, vol. 14. Springer Nature, 2023.","chicago":"Riedl, Michael, Isabelle D Mayer, Jack Merrin, Michael K Sixt, and Björn Hof. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” <i>Nature Communications</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41467-023-41432-1\">https://doi.org/10.1038/s41467-023-41432-1</a>.","ama":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. Synchronization in collectively moving inanimate and living active matter. <i>Nature Communications</i>. 2023;14. doi:<a href=\"https://doi.org/10.1038/s41467-023-41432-1\">10.1038/s41467-023-41432-1</a>","ista":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. 2023. Synchronization in collectively moving inanimate and living active matter. Nature Communications. 14, 5633.","mla":"Riedl, Michael, et al. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” <i>Nature Communications</i>, vol. 14, 5633, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s41467-023-41432-1\">10.1038/s41467-023-41432-1</a>.","short":"M. Riedl, I.D. Mayer, J. Merrin, M.K. Sixt, B. Hof, Nature Communications 14 (2023)."},"day":"13","ddc":["530","570"],"publication_status":"published","volume":14,"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"BjHo"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"_id":"14361","doi":"10.1038/s41467-023-41432-1","publication_identifier":{"eissn":["2041-1723"]},"scopus_import":"1","ec_funded":1,"file_date_updated":"2023-09-25T08:32:37Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Synchronization in collectively moving inanimate and living active matter","abstract":[{"lang":"eng","text":"Whether one considers swarming insects, flocking birds, or bacterial colonies, collective motion arises from the coordination of individuals and entails the adjustment of their respective velocities. In particular, in close confinements, such as those encountered by dense cell populations during development or regeneration, collective migration can only arise coordinately. Yet, how individuals unify their velocities is often not understood. Focusing on a finite number of cells in circular confinements, we identify waves of polymerizing actin that function as a pacemaker governing the speed of individual cells. We show that the onset of collective motion coincides with the synchronization of the wave nucleation frequencies across the population. Employing a simpler and more readily accessible mechanical model system of active spheres, we identify the synchronization of the individuals’ internal oscillators as one of the essential requirements to reach the corresponding collective state. The mechanical ‘toy’ experiment illustrates that the global synchronous state is achieved by nearest neighbor coupling. We suggest by analogy that local coupling and the synchronization of actin waves are essential for the emergent, self-organized motion of cell collectives."}],"pmid":1,"acknowledgement":"We thank K. O’Keeffe, E. Hannezo, P. Devreotes, C. Dessalles, and E. Martens for discussion and/or critical reading of the manuscript; the Bioimaging Facility of ISTA for excellent support, as well as the Life Science Facility and the Miba Machine Shop of ISTA. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S.","article_number":"5633","month":"09","type":"journal_article","external_id":{"isi":["001087583700030"],"pmid":["37704595"]},"year":"2023","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"type":"journal_article","month":"09","year":"2023","external_id":{"pmid":["37656776"],"isi":["001062110600003"]},"pmid":1,"abstract":[{"lang":"eng","text":"Immune responses rely on the rapid and coordinated migration of leukocytes. Whereas it is well established that single-cell migration is often guided by gradients of chemokines and other chemoattractants, it remains poorly understood how these gradients are generated, maintained, and modulated. By combining experimental data with theory on leukocyte chemotaxis guided by the G protein–coupled receptor (GPCR) CCR7, we demonstrate that in addition to its role as the sensory receptor that steers migration, CCR7 also acts as a generator and a modulator of chemotactic gradients. Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and ligand as part of the canonical GPCR desensitization response. We show that CCR7 internalization also acts as an effective sink for the chemoattractant, dynamically shaping the spatiotemporal distribution of the chemokine. This mechanism drives complex collective migration patterns, enabling DCs to create or sharpen chemotactic gradients. We further show that these self-generated gradients can sustain the long-range guidance of DCs, adapt collective migration patterns to the size and geometry of the environment, and provide a guidance cue for other comigrating cells. Such a dual role of CCR7 as a GPCR that both senses and consumes its ligand can thus provide a novel mode of cellular self-organization."}],"title":"CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"adc9584","acknowledgement":"We thank I. de Vries and the Scientific Service Units (Life Sciences, Bioimaging, Nanofabrication, Preclinical and Miba Machine Shop) of the Institute of Science and Technology Austria for excellent support, as well as all the rotation students assisting in the laboratory work (B. Zens, H. Schön, and D. Babic).\r\nThis work was supported by grants from the European Research Council under the European Union’s Horizon 2020 research to M.S. (grant agreement no. 724373) and to E.H. (grant agreement no. 851288), and a grant by the Austrian Science Fund (DK Nanocell W1250-B20) to M.S. J.A. was supported by the Jenny and Antti Wihuri Foundation and Research Council of Finland's Flagship Programme InFLAMES (decision number: 357910). M.C.U. was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754411.","scopus_import":"1","doi":"10.1126/sciimmunol.adc9584","publication_identifier":{"issn":["2470-9468"]},"issue":"87","ec_funded":1,"keyword":["General Medicine","Immunology"],"_id":"14274","department":[{"_id":"MiSi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"date_published":"2023-09-01T00:00:00Z","project":[{"grant_number":"724373","call_identifier":"H2020","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","call_identifier":"H2020"},{"name":"Nano-Analytics of Cellular Systems","_id":"265E2996-B435-11E9-9278-68D0E5697425","grant_number":"W01250-B20","call_identifier":"FWF"},{"grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"}],"corr_author":"1","volume":8,"ddc":["570"],"day":"01","publication_status":"published","article_processing_charge":"No","citation":{"ista":"Alanko JH, Ucar MC, Canigova N, Stopp JA, Schwarz J, Merrin J, Hannezo EB, Sixt MK. 2023. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. Science Immunology. 8(87), adc9584.","ama":"Alanko JH, Ucar MC, Canigova N, et al. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. <i>Science Immunology</i>. 2023;8(87). doi:<a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">10.1126/sciimmunol.adc9584</a>","mla":"Alanko, Jonna H., et al. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” <i>Science Immunology</i>, vol. 8, no. 87, adc9584, American Association for the Advancement of Science, 2023, doi:<a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">10.1126/sciimmunol.adc9584</a>.","short":"J.H. Alanko, M.C. Ucar, N. Canigova, J.A. Stopp, J. Schwarz, J. Merrin, E.B. Hannezo, M.K. Sixt, Science Immunology 8 (2023).","ieee":"J. H. Alanko <i>et al.</i>, “CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration,” <i>Science Immunology</i>, vol. 8, no. 87. American Association for the Advancement of Science, 2023.","apa":"Alanko, J. H., Ucar, M. C., Canigova, N., Stopp, J. A., Schwarz, J., Merrin, J., … Sixt, M. K. (2023). CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. <i>Science Immunology</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">https://doi.org/10.1126/sciimmunol.adc9584</a>","chicago":"Alanko, Jonna H, Mehmet C Ucar, Nikola Canigova, Julian A Stopp, Jan Schwarz, Jack Merrin, Edouard B Hannezo, and Michael K Sixt. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” <i>Science Immunology</i>. American Association for the Advancement of Science, 2023. <a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">https://doi.org/10.1126/sciimmunol.adc9584</a>."},"article_type":"original","oa_version":"Published Version","related_material":{"record":[{"status":"public","id":"14279","relation":"research_data"},{"status":"public","id":"19745","relation":"dissertation_contains"},{"relation":"dissertation_contains","id":"14697","status":"public"}]},"language":[{"iso":"eng"}],"publication":"Science Immunology","publisher":"American Association for the Advancement of Science","date_created":"2023-09-06T08:07:51Z","oa":1,"author":[{"first_name":"Jonna H","full_name":"Alanko, Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","last_name":"Alanko","orcid":"0000-0002-7698-3061"},{"last_name":"Ucar","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"id":"3795523E-F248-11E8-B48F-1D18A9856A87","full_name":"Canigova, Nikola","first_name":"Nikola","last_name":"Canigova","orcid":"0000-0002-8518-5926"},{"first_name":"Julian A","full_name":"Stopp, Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","last_name":"Stopp"},{"full_name":"Schwarz, Jan","first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"isi":1,"status":"public","main_file_link":[{"url":"https://doi.org/10.1126/sciimmunol.adc9584","open_access":"1"}],"quality_controlled":"1","intvolume":"         8","date_updated":"2026-06-23T22:31:00Z"},{"publication_identifier":{"eissn":["2691-1299"]},"issue":"4","doi":"10.1002/cpz1.407","scopus_import":"1","file_date_updated":"2022-05-02T08:16:10Z","department":[{"_id":"NanoFab"}],"_id":"11182","type":"journal_article","month":"04","year":"2022","external_id":{"pmid":["35384410"]},"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"title":"Quantifying the probing and selection of microenvironmental pores by motile immune cells","user_id":"0043cee0-e5fc-11ee-9736-f83bc23afbf0","pmid":1,"abstract":[{"lang":"eng","text":"Immune cells are constantly on the move through multicellular organisms to explore and respond to pathogens and other harmful insults. While moving, immune cells efficiently traverse microenvironments composed of tissue cells and extracellular fibers, which together form complex environments of various porosity, stiffness, topography, and chemical composition. In this protocol we describe experimental procedures to investigate immune cell migration through microenvironments of heterogeneous porosity. In particular, we describe micro-channels, micro-pillars, and collagen networks as cell migration paths with alternative pore size choices. Employing micro-channels or micro-pillars that divide at junctions into alternative paths with initially differentially sized pores allows us to precisely (1) measure the cellular translocation time through these porous path junctions, (2) quantify the cellular preference for individual pore sizes, and (3) image cellular components like the nucleus and the cytoskeleton. This reductionistic experimental setup thus can elucidate how immune cells perform decisions in complex microenvironments of various porosity like the interstitium. The setup further allows investigation of the underlying forces of cellular squeezing and the consequences of cellular deformation on the integrity of the cell and its organelles. As a complementary approach that does not require any micro-engineering expertise, we describe the usage of three-dimensional collagen networks with different pore sizes. Whereas we here focus on dendritic cells as a model for motile immune cells, the described protocols are versatile as they are also applicable for other immune cell types like neutrophils and non-immune cell types such as mesenchymal and cancer cells. In summary, we here describe protocols to identify the mechanisms and principles of cellular probing, decision making, and squeezing during cellular movement through microenvironments of heterogeneous porosity."}],"acknowledgement":"We thank Kasia Stefanowski for excellent technical assistance, and the Core Facility Bioimaging of the Biomedical Center (BMC) of the Ludwig-Maximilian University for excellent support. We gratefully acknowledge financial support from the Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (to J.R), from the DFG (Collaborative Research Center SFB914, project A12; and Priority Programme SPP2332, project 492014049; both to J.R) and from the LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (to J.R).\r\nOpen access funding enabled and organized by Projekt DEAL.","article_number":"e407","oa_version":"Published Version","article_type":"original","publisher":"Wiley","date_created":"2022-04-17T22:01:46Z","language":[{"iso":"eng"}],"publication":"Current Protocols","status":"public","author":[{"last_name":"Kroll","first_name":"Janina","full_name":"Kroll, Janina"},{"full_name":"Ruiz-Fernandez, Mauricio J.A.","first_name":"Mauricio J.A.","last_name":"Ruiz-Fernandez"},{"first_name":"Malte B.","full_name":"Braun, Malte B.","last_name":"Braun"},{"full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","last_name":"Merrin"},{"orcid":"0000-0003-2856-3369","last_name":"Renkawitz","first_name":"Jörg","full_name":"Renkawitz, Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87"}],"oa":1,"file":[{"content_type":"application/pdf","file_size":2142703,"relation":"main_file","creator":"dernst","date_updated":"2022-05-02T08:16:10Z","success":1,"file_name":"2022_CurrentProtocols_Kroll.pdf","checksum":"72152d005c367777f6cf2f6a477f0d52","file_id":"11347","access_level":"open_access","date_created":"2022-05-02T08:16:10Z"}],"OA_place":"publisher","date_updated":"2024-10-14T13:16:54Z","quality_controlled":"1","intvolume":"         2","date_published":"2022-04-05T00:00:00Z","ddc":["570"],"day":"05","publication_status":"published","article_processing_charge":"No","citation":{"ieee":"J. Kroll, M. J. A. Ruiz-Fernandez, M. B. Braun, J. Merrin, and J. Renkawitz, “Quantifying the probing and selection of microenvironmental pores by motile immune cells,” <i>Current Protocols</i>, vol. 2, no. 4. Wiley, 2022.","chicago":"Kroll, Janina, Mauricio J.A. Ruiz-Fernandez, Malte B. Braun, Jack Merrin, and Jörg Renkawitz. “Quantifying the Probing and Selection of Microenvironmental Pores by Motile Immune Cells.” <i>Current Protocols</i>. Wiley, 2022. <a href=\"https://doi.org/10.1002/cpz1.407\">https://doi.org/10.1002/cpz1.407</a>.","apa":"Kroll, J., Ruiz-Fernandez, M. J. A., Braun, M. B., Merrin, J., &#38; Renkawitz, J. (2022). Quantifying the probing and selection of microenvironmental pores by motile immune cells. <i>Current Protocols</i>. Wiley. <a href=\"https://doi.org/10.1002/cpz1.407\">https://doi.org/10.1002/cpz1.407</a>","ama":"Kroll J, Ruiz-Fernandez MJA, Braun MB, Merrin J, Renkawitz J. Quantifying the probing and selection of microenvironmental pores by motile immune cells. <i>Current Protocols</i>. 2022;2(4). doi:<a href=\"https://doi.org/10.1002/cpz1.407\">10.1002/cpz1.407</a>","ista":"Kroll J, Ruiz-Fernandez MJA, Braun MB, Merrin J, Renkawitz J. 2022. Quantifying the probing and selection of microenvironmental pores by motile immune cells. Current Protocols. 2(4), e407.","mla":"Kroll, Janina, et al. “Quantifying the Probing and Selection of Microenvironmental Pores by Motile Immune Cells.” <i>Current Protocols</i>, vol. 2, no. 4, e407, Wiley, 2022, doi:<a href=\"https://doi.org/10.1002/cpz1.407\">10.1002/cpz1.407</a>.","short":"J. Kroll, M.J.A. Ruiz-Fernandez, M.B. Braun, J. Merrin, J. Renkawitz, Current Protocols 2 (2022)."},"OA_type":"hybrid","volume":2,"has_accepted_license":"1"},{"corr_author":"1","project":[{"_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","name":"Tribocharge: a multi-scale approach to an enduring problem in physics","grant_number":"949120","call_identifier":"H2020"}],"date_published":"2022-12-29T00:00:00Z","article_processing_charge":"No","citation":{"mla":"Pertl, Felix, et al. “Quantifying Nanoscale Charge Density Features of Contact-Charged Surfaces with an FEM/KPFM-Hybrid Approach.” <i>Physical Review Materials</i>, vol. 6, no. 12, 125605, American Physical Society, 2022, doi:<a href=\"https://doi.org/10.1103/PhysRevMaterials.6.125605\">10.1103/PhysRevMaterials.6.125605</a>.","ista":"Pertl F, Sobarzo Ponce JCA, Shafeek LB, Cramer T, Waitukaitis SR. 2022. Quantifying nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid approach. Physical Review Materials. 6(12), 125605.","ama":"Pertl F, Sobarzo Ponce JCA, Shafeek LB, Cramer T, Waitukaitis SR. Quantifying nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid approach. <i>Physical Review Materials</i>. 2022;6(12). doi:<a href=\"https://doi.org/10.1103/PhysRevMaterials.6.125605\">10.1103/PhysRevMaterials.6.125605</a>","short":"F. Pertl, J.C.A. Sobarzo Ponce, L.B. Shafeek, T. Cramer, S.R. Waitukaitis, Physical Review Materials 6 (2022).","ieee":"F. Pertl, J. C. A. Sobarzo Ponce, L. B. Shafeek, T. Cramer, and S. R. Waitukaitis, “Quantifying nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid approach,” <i>Physical Review Materials</i>, vol. 6, no. 12. American Physical Society, 2022.","chicago":"Pertl, Felix, Juan Carlos A Sobarzo Ponce, Lubuna B Shafeek, Tobias Cramer, and Scott R Waitukaitis. “Quantifying Nanoscale Charge Density Features of Contact-Charged Surfaces with an FEM/KPFM-Hybrid Approach.” <i>Physical Review Materials</i>. American Physical Society, 2022. <a href=\"https://doi.org/10.1103/PhysRevMaterials.6.125605\">https://doi.org/10.1103/PhysRevMaterials.6.125605</a>.","apa":"Pertl, F., Sobarzo Ponce, J. C. A., Shafeek, L. B., Cramer, T., &#38; Waitukaitis, S. R. (2022). Quantifying nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid approach. <i>Physical Review Materials</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevMaterials.6.125605\">https://doi.org/10.1103/PhysRevMaterials.6.125605</a>"},"day":"29","publication_status":"published","volume":6,"oa_version":"Preprint","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"20203"}]},"arxiv":1,"article_type":"original","publisher":"American Physical Society","date_created":"2023-01-08T23:00:53Z","publication":"Physical Review Materials","language":[{"iso":"eng"}],"status":"public","isi":1,"author":[{"orcid":"0000-0003-0463-5794","last_name":"Pertl","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","full_name":"Pertl, Felix","first_name":"Felix"},{"last_name":"Sobarzo Ponce","full_name":"Sobarzo Ponce, Juan Carlos A","first_name":"Juan Carlos A","id":"4B807D68-AE37-11E9-AC72-31CAE5697425"},{"last_name":"Shafeek","orcid":"0000-0001-7180-6050","full_name":"Shafeek, Lubuna B","first_name":"Lubuna B","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Cramer","first_name":"Tobias","full_name":"Cramer, Tobias"},{"id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","first_name":"Scott R","full_name":"Waitukaitis, Scott R","last_name":"Waitukaitis","orcid":"0000-0002-2299-3176"}],"oa":1,"date_updated":"2026-04-07T11:50:54Z","main_file_link":[{"url":" https://doi.org/10.48550/arXiv.2209.01889","open_access":"1"}],"intvolume":"         6","quality_controlled":"1","month":"12","type":"journal_article","external_id":{"arxiv":["2209.01889"],"isi":["000908384800001"]},"year":"2022","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Quantifying nanoscale charge density features of contact-charged surfaces with an FEM/KPFM-hybrid approach","abstract":[{"lang":"eng","text":"Kelvin probe force microscopy (KPFM) is a powerful tool for studying contact electrification (CE) at the nanoscale, but converting KPFM voltage maps to charge density maps is nontrivial due to long-range forces and complex system geometry. Here we present a strategy using finite-element method (FEM) simulations to determine the Green's function of the KPFM probe/insulator/ground system, which allows us to quantitatively extract surface charge. Testing our approach with synthetic data, we find that accounting for the atomic force microscope (AFM) tip, cone, and cantilever is necessary to recover a known input and that existing methods lead to gross miscalculation or even the incorrect sign of the underlying charge. Applying it to experimental data, we demonstrate its capacity to extract realistic surface charge densities and fine details from contact-charged surfaces. Our method gives a straightforward recipe to convert qualitative KPFM voltage data into quantitative charge data over a range of experimental conditions, enabling quantitative CE at the nanoscale."}],"acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant Agreement\r\nNo. 949120). This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine\r\nShop, the Nanofabrication Facility, and the Scientific Computing Facility. We thank F. Stumpf from Park Systems for useful discussions and support with scanning probe microscopy.\r\nF.P. and J.C.S. contributed equally to this work.","article_number":"125605","publication_identifier":{"eissn":["2475-9953"]},"issue":"12","doi":"10.1103/PhysRevMaterials.6.125605","scopus_import":"1","ec_funded":1,"department":[{"_id":"ScWa"},{"_id":"NanoFab"}],"_id":"12109","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"}]},{"language":[{"iso":"eng"}],"publication":"Chaos: An Interdisciplinary Journal of Nonlinear Science","publisher":"AIP Publishing","date_created":"2023-01-16T09:58:16Z","article_type":"original","arxiv":1,"oa_version":"Published Version","intvolume":"        32","quality_controlled":"1","date_updated":"2025-06-11T13:41:34Z","oa":1,"author":[{"last_name":"Choueiri","id":"448BD5BC-F248-11E8-B48F-1D18A9856A87","full_name":"Choueiri, George H","first_name":"George H"},{"first_name":"Balachandra","full_name":"Suri, Balachandra","id":"47A5E706-F248-11E8-B48F-1D18A9856A87","last_name":"Suri"},{"first_name":"Jack","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"last_name":"Serbyn","orcid":"0000-0002-2399-5827","id":"47809E7E-F248-11E8-B48F-1D18A9856A87","first_name":"Maksym","full_name":"Serbyn, Maksym"},{"id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn","full_name":"Hof, Björn","last_name":"Hof","orcid":"0000-0003-2057-2754"},{"orcid":"0000-0003-0423-5010","last_name":"Budanur","first_name":"Nazmi B","full_name":"Budanur, Nazmi B","id":"3EA1010E-F248-11E8-B48F-1D18A9856A87"}],"isi":1,"file":[{"content_type":"application/pdf","file_size":3209644,"date_updated":"2023-01-30T09:41:12Z","success":1,"relation":"main_file","creator":"dernst","file_id":"12445","file_name":"2022_Chaos_Choueiri.pdf","checksum":"17881eff8b21969359a2dd64620120ba","date_created":"2023-01-30T09:41:12Z","access_level":"open_access"}],"status":"public","volume":32,"day":"26","ddc":["530"],"publication_status":"published","citation":{"ieee":"G. H. Choueiri, B. Suri, J. Merrin, M. Serbyn, B. Hof, and N. B. Budanur, “Crises and chaotic scattering in hydrodynamic pilot-wave experiments,” <i>Chaos: An Interdisciplinary Journal of Nonlinear Science</i>, vol. 32, no. 9. AIP Publishing, 2022.","chicago":"Choueiri, George H, Balachandra Suri, Jack Merrin, Maksym Serbyn, Björn Hof, and Nazmi B Budanur. “Crises and Chaotic Scattering in Hydrodynamic Pilot-Wave Experiments.” <i>Chaos: An Interdisciplinary Journal of Nonlinear Science</i>. AIP Publishing, 2022. <a href=\"https://doi.org/10.1063/5.0102904\">https://doi.org/10.1063/5.0102904</a>.","apa":"Choueiri, G. H., Suri, B., Merrin, J., Serbyn, M., Hof, B., &#38; Budanur, N. B. (2022). Crises and chaotic scattering in hydrodynamic pilot-wave experiments. <i>Chaos: An Interdisciplinary Journal of Nonlinear Science</i>. AIP Publishing. <a href=\"https://doi.org/10.1063/5.0102904\">https://doi.org/10.1063/5.0102904</a>","short":"G.H. Choueiri, B. Suri, J. Merrin, M. Serbyn, B. Hof, N.B. Budanur, Chaos: An Interdisciplinary Journal of Nonlinear Science 32 (2022).","ista":"Choueiri GH, Suri B, Merrin J, Serbyn M, Hof B, Budanur NB. 2022. Crises and chaotic scattering in hydrodynamic pilot-wave experiments. Chaos: An Interdisciplinary Journal of Nonlinear Science. 32(9), 093138.","ama":"Choueiri GH, Suri B, Merrin J, Serbyn M, Hof B, Budanur NB. Crises and chaotic scattering in hydrodynamic pilot-wave experiments. <i>Chaos: An Interdisciplinary Journal of Nonlinear Science</i>. 2022;32(9). doi:<a href=\"https://doi.org/10.1063/5.0102904\">10.1063/5.0102904</a>","mla":"Choueiri, George H., et al. “Crises and Chaotic Scattering in Hydrodynamic Pilot-Wave Experiments.” <i>Chaos: An Interdisciplinary Journal of Nonlinear Science</i>, vol. 32, no. 9, 093138, AIP Publishing, 2022, doi:<a href=\"https://doi.org/10.1063/5.0102904\">10.1063/5.0102904</a>."},"article_processing_charge":"No","date_published":"2022-09-26T00:00:00Z","has_accepted_license":"1","file_date_updated":"2023-01-30T09:41:12Z","scopus_import":"1","doi":"10.1063/5.0102904","publication_identifier":{"eissn":["1089-7682"],"issn":["1054-1500"]},"issue":"9","_id":"12259","department":[{"_id":"MaSe"},{"_id":"BjHo"},{"_id":"NanoFab"}],"keyword":["Applied Mathematics","General Physics and Astronomy","Mathematical Physics","Statistical and Nonlinear Physics"],"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2022","external_id":{"isi":["000861009600005"],"pmid":["36182399"],"arxiv":["2206.01531"]},"type":"journal_article","month":"09","article_number":"093138","acknowledgement":"This work was partially funded by the Institute of Science and Technology Austria Interdisciplinary Project Committee Grant “Pilot-Wave Hydrodynamics: Chaos and Quantum Analogies.”","pmid":1,"abstract":[{"lang":"eng","text":"Theoretical foundations of chaos have been predominantly laid out for finite-dimensional dynamical systems, such as the three-body problem in classical mechanics and the Lorenz model in dissipative systems. In contrast, many real-world chaotic phenomena, e.g., weather, arise in systems with many (formally infinite) degrees of freedom, which limits direct quantitative analysis of such systems using chaos theory. In the present work, we demonstrate that the hydrodynamic pilot-wave systems offer a bridge between low- and high-dimensional chaotic phenomena by allowing for a systematic study of how the former connects to the latter. Specifically, we present experimental results, which show the formation of low-dimensional chaotic attractors upon destabilization of regular dynamics and a final transition to high-dimensional chaos via the merging of distinct chaotic regions through a crisis bifurcation. Moreover, we show that the post-crisis dynamics of the system can be rationalized as consecutive scatterings from the nonattracting chaotic sets with lifetimes following exponential distributions. "}],"title":"Crises and chaotic scattering in hydrodynamic pilot-wave experiments","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}]