---
_id: '2018'
abstract:
- lang: eng
text: Synaptic cell adhesion molecules are increasingly gaining attention for conferring
specific properties to individual synapses. Netrin-G1 and netrin-G2 are trans-synaptic
adhesion molecules that distribute on distinct axons, and their presence restricts
the expression of their cognate receptors, NGL1 and NGL2, respectively, to specific
subdendritic segments of target neurons. However, the neural circuits and functional
roles of netrin-G isoform complexes remain unclear. Here, we use netrin-G-KO and
NGL-KO mice to reveal that netrin-G1/NGL1 and netrin-G2/NGL2 interactions specify
excitatory synapses in independent hippocampal pathways. In the hippocampal CA1
area, netrin-G1/NGL1 and netrin-G2/NGL2 were expressed in the temporoammonic and
Schaffer collateral pathways, respectively. The lack of presynaptic netrin-Gs
led to the dispersion of NGLs from postsynaptic membranes. In accord, netrin-G
mutant synapses displayed opposing phenotypes in long-term and short-term plasticity
through discrete biochemical pathways. The plasticity phenotypes in netrin-G-KOs
were phenocopied in NGL-KOs, with a corresponding loss of netrin-Gs from presynaptic
membranes. Our findings show that netrin-G/NGL interactions differentially control
synaptic plasticity in distinct circuits via retrograde signaling mechanisms and
explain how synaptic inputs are diversified to control neuronal activity.
acknowledgement: This work was supported by “Funding Program for World-Leading Innovative
R&D on Science and Technology (FIRST Program)” initiated by the Council for Science
and Technology Policy.
article_processing_charge: No
article_type: original
author:
- first_name: Hiroshi
full_name: Matsukawa, Hiroshi
last_name: Matsukawa
- first_name: Sachiko
full_name: Akiyoshi Nishimura, Sachiko
last_name: Akiyoshi Nishimura
- first_name: Qi
full_name: Zhang, Qi
last_name: Zhang
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Kazuhiko
full_name: Yamaguchi, Kazuhiko
last_name: Yamaguchi
- first_name: Hiromichi
full_name: Goto, Hiromichi
last_name: Goto
- first_name: Kunio
full_name: Yaguchi, Kunio
last_name: Yaguchi
- first_name: Tsutomu
full_name: Hashikawa, Tsutomu
last_name: Hashikawa
- first_name: Chie
full_name: Sano, Chie
last_name: Sano
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Toshiaki
full_name: Nakashiba, Toshiaki
last_name: Nakashiba
- first_name: Shigeyoshi
full_name: Itohara, Shigeyoshi
last_name: Itohara
citation:
ama: Matsukawa H, Akiyoshi Nishimura S, Zhang Q, et al. Netrin-G/NGL complexes encode
functional synaptic diversification. Journal of Neuroscience. 2014;34(47):15779-15792.
doi:10.1523/JNEUROSCI.1141-14.2014
apa: Matsukawa, H., Akiyoshi Nishimura, S., Zhang, Q., Luján, R., Yamaguchi, K.,
Goto, H., … Itohara, S. (2014). Netrin-G/NGL complexes encode functional synaptic
diversification. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.1141-14.2014
chicago: Matsukawa, Hiroshi, Sachiko Akiyoshi Nishimura, Qi Zhang, Rafael Luján,
Kazuhiko Yamaguchi, Hiromichi Goto, Kunio Yaguchi, et al. “Netrin-G/NGL Complexes
Encode Functional Synaptic Diversification.” Journal of Neuroscience. Society
for Neuroscience, 2014. https://doi.org/10.1523/JNEUROSCI.1141-14.2014.
ieee: H. Matsukawa et al., “Netrin-G/NGL complexes encode functional synaptic
diversification,” Journal of Neuroscience, vol. 34, no. 47. Society for
Neuroscience, pp. 15779–15792, 2014.
ista: Matsukawa H, Akiyoshi Nishimura S, Zhang Q, Luján R, Yamaguchi K, Goto H,
Yaguchi K, Hashikawa T, Sano C, Shigemoto R, Nakashiba T, Itohara S. 2014. Netrin-G/NGL
complexes encode functional synaptic diversification. Journal of Neuroscience.
34(47), 15779–15792.
mla: Matsukawa, Hiroshi, et al. “Netrin-G/NGL Complexes Encode Functional Synaptic
Diversification.” Journal of Neuroscience, vol. 34, no. 47, Society for
Neuroscience, 2014, pp. 15779–92, doi:10.1523/JNEUROSCI.1141-14.2014.
short: H. Matsukawa, S. Akiyoshi Nishimura, Q. Zhang, R. Luján, K. Yamaguchi, H.
Goto, K. Yaguchi, T. Hashikawa, C. Sano, R. Shigemoto, T. Nakashiba, S. Itohara,
Journal of Neuroscience 34 (2014) 15779–15792.
date_created: 2018-12-11T11:55:14Z
date_published: 2014-11-19T00:00:00Z
date_updated: 2025-09-29T12:00:37Z
day: '19'
ddc:
- '570'
department:
- _id: RySh
doi: 10.1523/JNEUROSCI.1141-14.2014
external_id:
isi:
- '000345907500026'
pmid:
- '25411505'
file:
- access_level: open_access
checksum: 6913e9bc26e9fc1c0441a739a4199229
content_type: application/pdf
creator: dernst
date_created: 2022-05-24T08:41:41Z
date_updated: 2022-05-24T08:41:41Z
file_id: '11410'
file_name: 2014_JournNeuroscience_Matsukawa.pdf
file_size: 3963728
relation: main_file
success: 1
file_date_updated: 2022-05-24T08:41:41Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '47'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 15779 - 15792
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
eissn:
- 1529-2401
issn:
- 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '5054'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Netrin-G/NGL complexes encode functional synaptic diversification
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2014'
...
---
_id: '2064'
abstract:
- lang: eng
text: We examined the synaptic structure, quantity, and distribution of α-amino-3-hydroxy-5-methylisoxazole-4-propionic
acid (AMPA)- and N-methyl-D-aspartate (NMDA)-type glutamate receptors (AMPARs
and NMDARs, respectively) in rat cochlear nuclei by a highly sensitive freeze-fracture
replica labeling technique. Four excitatory synapses formed by two distinct inputs,
auditory nerve (AN) and parallel fibers (PF), on different cell types were analyzed.
These excitatory synapse types included AN synapses on bushy cells (AN-BC synapses)
and fusiform cells (AN-FC synapses) and PF synapses on FC (PF-FC synapses) and
cartwheel cell spines (PF-CwC synapses). Immunogold labeling revealed differences
in synaptic structure as well as AMPAR and NMDAR number and/or density in both
AN and PF synapses, indicating a target-dependent organization. The immunogold
receptor labeling also identified differences in the synaptic organization of
FCs based on AN or PF connections, indicating an input-dependent organization
in FCs. Among the four excitatory synapse types, the AN-BC synapses were the smallest
and had the most densely packed intramembrane particles (IMPs), whereas the PF-CwC
synapses were the largest and had sparsely packed IMPs. All four synapse types
showed positive correlations between the IMP-cluster area and the AMPAR number,
indicating a common intrasynapse-type relationship for glutamatergic synapses.
Immunogold particles for AMPARs were distributed over the entire area of individual
AN synapses; PF synapses often showed synaptic areas devoid of labeling. The gold-labeling
for NMDARs occurred in a mosaic fashion, with less positive correlations between
the IMP-cluster area and the NMDAR number. Our observations reveal target- and
input-dependent features in the structure, number, and organization of AMPARs
and NMDARs in AN and PF synapses.
acknowledgement: "National Institutes of Health (NIH) Grant Number: 1R01DC013048‐0;
Biotechnology and Biological Sciences Research Council, UK Grant Number: BB/J015938/1\r\n"
article_processing_charge: No
author:
- first_name: Maía
full_name: Rubio, Maía
last_name: Rubio
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Naomi
full_name: Kamasawa, Naomi
last_name: Kamasawa
- first_name: Cheryl
full_name: Clarkson, Cheryl
last_name: Clarkson
- first_name: Elek
full_name: Molnár, Elek
last_name: Molnár
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Rubio M, Fukazawa Y, Kamasawa N, Clarkson C, Molnár E, Shigemoto R. Target-
and input-dependent organization of AMPA and NMDA receptors in synaptic connections
of the cochlear nucleus. Journal of Comparative Neurology. 2014;522(18):4023-4042.
doi:10.1002/cne.23654
apa: Rubio, M., Fukazawa, Y., Kamasawa, N., Clarkson, C., Molnár, E., & Shigemoto,
R. (2014). Target- and input-dependent organization of AMPA and NMDA receptors
in synaptic connections of the cochlear nucleus. Journal of Comparative Neurology.
Wiley-Blackwell. https://doi.org/10.1002/cne.23654
chicago: Rubio, Maía, Yugo Fukazawa, Naomi Kamasawa, Cheryl Clarkson, Elek Molnár,
and Ryuichi Shigemoto. “Target- and Input-Dependent Organization of AMPA and NMDA
Receptors in Synaptic Connections of the Cochlear Nucleus.” Journal of Comparative
Neurology. Wiley-Blackwell, 2014. https://doi.org/10.1002/cne.23654.
ieee: M. Rubio, Y. Fukazawa, N. Kamasawa, C. Clarkson, E. Molnár, and R. Shigemoto,
“Target- and input-dependent organization of AMPA and NMDA receptors in synaptic
connections of the cochlear nucleus,” Journal of Comparative Neurology,
vol. 522, no. 18. Wiley-Blackwell, pp. 4023–4042, 2014.
ista: Rubio M, Fukazawa Y, Kamasawa N, Clarkson C, Molnár E, Shigemoto R. 2014.
Target- and input-dependent organization of AMPA and NMDA receptors in synaptic
connections of the cochlear nucleus. Journal of Comparative Neurology. 522(18),
4023–4042.
mla: Rubio, Maía, et al. “Target- and Input-Dependent Organization of AMPA and NMDA
Receptors in Synaptic Connections of the Cochlear Nucleus.” Journal of Comparative
Neurology, vol. 522, no. 18, Wiley-Blackwell, 2014, pp. 4023–42, doi:10.1002/cne.23654.
short: M. Rubio, Y. Fukazawa, N. Kamasawa, C. Clarkson, E. Molnár, R. Shigemoto,
Journal of Comparative Neurology 522 (2014) 4023–4042.
date_created: 2018-12-11T11:55:30Z
date_published: 2014-07-29T00:00:00Z
date_updated: 2025-09-29T11:47:23Z
day: '29'
department:
- _id: RySh
doi: 10.1002/cne.23654
external_id:
isi:
- '000343973100005'
intvolume: ' 522'
isi: 1
issue: '18'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198489/
month: '07'
oa: 1
oa_version: Submitted Version
page: 4023 - 4042
publication: Journal of Comparative Neurology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4974'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Target- and input-dependent organization of AMPA and NMDA receptors in synaptic
connections of the cochlear nucleus
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 522
year: '2014'
...