[{"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","project":[{"call_identifier":"H2020","name":"Epidemics in ant societies on a chip","grant_number":"771402","_id":"2649B4DE-B435-11E9-9278-68D0E5697425"}],"has_accepted_license":"1","date_published":"2025-12-01T00:00:00Z","date_updated":"2026-04-28T12:57:04Z","year":"2025","oa_version":"Published Version","publisher":"Springer Nature","type":"journal_article","day":"01","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","OA_type":"gold","doi":"10.1038/s41467-025-66175-z","OA_place":"publisher","ddc":["570"],"article_processing_charge":"Yes","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/ants-signal-deadly-infection/","description":"News on ISTA website"}],"record":[{"id":"20471","status":"public","relation":"research_data"}]},"PlanS_conform":"1","citation":{"chicago":"Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse, Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>.","ista":"Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic disease signalling in ant colonies. Nature Communications. 16, 10511.","ama":"Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>","apa":"Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L., Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>","ieee":"E. Dawson <i>et al.</i>, “Altruistic disease signalling in ant colonies,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","mla":"Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>, vol. 16, 10511, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>.","short":"E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J. Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S. Cremer, Nature Communications 16 (2025)."},"_id":"18892","month":"12","title":"Altruistic disease signalling in ant colonies","author":[{"full_name":"Dawson, Erika","last_name":"Dawson","first_name":"Erika","id":"31B4E2D0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hönigsberger","full_name":"Hönigsberger, Michaela","first_name":"Michaela","id":"953894f3-25bd-11ec-8556-f70a9d38ef60"},{"full_name":"Kampleitner, Niklas","last_name":"Kampleitner","first_name":"Niklas","id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Grasse, Anna V","last_name":"Grasse","first_name":"Anna V","id":"406F989C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Lindorfer","full_name":"Lindorfer, Lukas","id":"85f0e6d3-06b3-11ec-8982-8c5049fa4455","first_name":"Lukas"},{"first_name":"Jennifer","id":"7bc2734a-e2c6-11ea-9824-a2ed5f0662a8","last_name":"Robb","full_name":"Robb, Jennifer"},{"id":"0344bfb9-3feb-11ee-87e9-c27edc800bcd","first_name":"Farnaz","last_name":"Beikzadeh Abbasi","full_name":"Beikzadeh Abbasi, Farnaz"},{"last_name":"Strahodinsky","full_name":"Strahodinsky, Florian","id":"979E35EE-C996-11E9-8C7C-CF13E6697425","first_name":"Florian"},{"full_name":"Leitner, Hanna","last_name":"Leitner","first_name":"Hanna","id":"8fc5c6f6-5903-11ec-abad-c83f046253e7"},{"last_name":"Rajendran","full_name":"Rajendran, Harikrishnan","first_name":"Harikrishnan","id":"876b6b34-8ff4-11ec-97c9-8d95a7aae416"},{"first_name":"Thomas","full_name":"Schmitt, Thomas","last_name":"Schmitt"},{"orcid":"0000-0002-2193-3868","last_name":"Cremer","full_name":"Cremer, Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","first_name":"Sylvia"}],"file_date_updated":"2025-12-15T13:30:33Z","scopus_import":"1","publication":"Nature Communications","intvolume":"        16","ec_funded":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.27.582277"}],"pmid":1,"file":[{"date_created":"2025-12-15T13:30:33Z","file_id":"20826","file_name":"2025_NatureComm_Dawson.pdf","creator":"dernst","file_size":805323,"checksum":"06244623bb7611c636652ecbc4787889","content_type":"application/pdf","date_updated":"2025-12-15T13:30:33Z","success":1,"relation":"main_file","access_level":"open_access"}],"acknowledgement":"We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant collection. We also thank the Social Immunity team, in particular David Moreno Martínez, Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular support, as well as Friedrich Fochler for technical support with the chemical analysis, and the ISTA Lab Support Facility, including the mass spectrometry unit, for general and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull and Michael Sixt for project discussion, and the Social Immunity team for comments on the manuscript. The study was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation Programme (No. 771402; EPIDEMICSonCHIP) to SC.","DOAJ_listed":"1","oa":1,"publication_identifier":{"eissn":["2041-1723"]},"date_created":"2025-01-27T11:28:05Z","department":[{"_id":"SyCr"},{"_id":"LifeSc"}],"external_id":{"pmid":["41330896"]},"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published","article_number":"10511","license":"https://creativecommons.org/licenses/by/4.0/","abstract":[{"text":"Sick individuals often conceal their disease status to group members, thereby preventing social exclusion or aggression. Here we show by behavioural, chemical, immunological and infection load analyses that sick ant pupae instead actively emit a chemical signal that in itself is sufficient to trigger their own destruction by colony members. In our experiments, this altruistic disease-signalling was performed only by worker but not queen pupae. The lack of signalling by queen pupae did not constitute cheating behaviour, but reflected their superior immune capabilities. Worker pupae suffered from extensive pathogen replication whereas queen pupae were able to restrain their infection. Our data suggest the evolution of a finely-tuned signalling system in which it is not the induction of an individual’s immune response, but rather its failure to overcome the infection, that triggers pupal signalling for sacrifice. This demonstrates a balanced interplay between individual and social immunity that efficiently achieves whole-colony health.","lang":"eng"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"}],"volume":16,"quality_controlled":"1"},{"date_updated":"2026-04-28T13:18:33Z","oa_version":"Published Version","year":"2025","project":[{"_id":"8df062be-16d5-11f0-9cad-f559b6612c7e","grant_number":"P37169","name":"Singlet oxygen in non-aqueous oxygen redox chemistry"},{"name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","grant_number":"CZI01"}],"date_published":"2025-10-16T00:00:00Z","has_accepted_license":"1","page":"601–605","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","issue":"8085","day":"16","type":"journal_article","publisher":"Springer Nature","ddc":["540"],"article_type":"original","OA_type":"hybrid","OA_place":"publisher","doi":"10.1038/s41586-025-09587-7","month":"10","title":"Marcus kinetics control singlet and triplet oxygen evolving from superoxide","_id":"17468","PlanS_conform":"1","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/taming-the-bad-oxygen/","description":"News on ISTA website"}]},"citation":{"short":"S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025) 601–605.","mla":"Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>, vol. 646, no. 8085, Springer Nature, 2025, pp. 601–605, doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>.","ieee":"S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics control singlet and triplet oxygen evolving from superoxide,” <i>Nature</i>, vol. 646, no. 8085. Springer Nature, pp. 601–605, 2025.","ama":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. 2025;646(8085):601–605. doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>","apa":"Mondal, S., Nguyen, H. T. K., Hauschild, R., &#38; Freunberger, S. A. (2025). Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>","chicago":"Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>.","ista":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605."},"article_processing_charge":"Yes (via OA deal)","intvolume":"       646","isi":1,"author":[{"last_name":"Mondal","full_name":"Mondal, Soumyadip","first_name":"Soumyadip","id":"d25d21ef-dc8d-11ea-abe3-ec4576307f48"},{"full_name":"Nguyen, Huyen T.K.","last_name":"Nguyen","first_name":"Huyen T.K."},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Freunberger, Stefan Alexander","last_name":"Freunberger","first_name":"Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","orcid":"0000-0003-2902-5319"}],"scopus_import":"1","publication":"Nature","file_date_updated":"2025-10-20T10:26:13Z","oa":1,"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"pmid":1,"acknowledgement":"S.A.F. thanks the Institute of Science and Technology Austria (ISTA) for the support. The Scientific Service Units of ISTA supported this research through resources provided by the Imaging and Optics Facility, the Lab Support Facility, the Miba Machine Shop and Scientific Computing. This research was partly funded by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open access purposes, the author has applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. R.H. acknowledges funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989). H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life and R. Jethwa for proofreading. Open access funding was provided by ISTA.","file":[{"file_name":"2025_Nature_Mondal.pdf","creator":"dernst","date_created":"2025-10-20T10:26:13Z","file_id":"20500","file_size":3809247,"checksum":"b507ddd23df0388aa65d04dc9b00fe3d","content_type":"application/pdf","date_updated":"2025-10-20T10:26:13Z","success":1,"access_level":"open_access","relation":"main_file"}],"publication_status":"published","corr_author":"1","language":[{"iso":"eng"}],"date_created":"2024-08-29T10:40:23Z","external_id":{"pmid":["41044415"],"isi":["001586378900001"]},"department":[{"_id":"StFr"},{"_id":"Bio"}],"quality_controlled":"1","volume":646,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"abstract":[{"lang":"eng","text":"Oxygen redox chemistry is central to life1 and many human-made technologies, such as in energy storage2,3,4. The large energy gain from oxygen redox reactions is often connected with the occurrence of harmful reactive oxygen species3,5,6. Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which may evolve from superoxide. However, the factors determining the formation of singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown. Here we report that the release of triplet or singlet oxygen is governed by individual Marcus normal and inverted region behaviour. We found that as the driving force for the reaction increases, the initially dominant evolution of triplet oxygen slows down, and singlet oxygen evolution becomes predominant with higher maximum kinetics. This behaviour also applies to the widely observed superoxide disproportionation, in which one superoxide is oxidized by another, in both non-aqueous and aqueous systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet oxygen yields governed by these conditions are relevant, for example, in batteries or cellular organelles in which superoxide forms. Our findings suggest ways to understand and control spin states and kinetics in oxygen redox chemistry, with implications for fields, including life sciences, pure chemistry and energy storage."}]},{"file":[{"access_level":"open_access","relation":"main_file","success":1,"date_updated":"2025-07-31T08:00:33Z","checksum":"0c725123dca7797c682609bff2c4c5ac","content_type":"application/pdf","file_size":13514646,"file_name":"2025_NatureImmunology_ReisRodrigues.pdf","creator":"dernst","date_created":"2025-07-31T08:00:33Z","file_id":"20096"}],"acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","pmid":1,"publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"oa":1,"publication":"Nature Immunology","file_date_updated":"2025-07-31T08:00:33Z","scopus_import":"1","author":[{"first_name":"Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues","full_name":"Dos Reis Rodrigues, Patricia","orcid":"0000-0003-1681-508X"},{"first_name":"Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","full_name":"Avellaneda Sarrió, Mario","last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X"},{"first_name":"Nikola","id":"3795523E-F248-11E8-B48F-1D18A9856A87","last_name":"Canigova","full_name":"Canigova, Nikola","orcid":"0000-0002-8518-5926"},{"full_name":"Gärtner, Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","orcid":"0000-0001-6120-3723"},{"full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518"},{"orcid":"0000-0003-4844-6311","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","first_name":"Michael","last_name":"Riedl","full_name":"Riedl, Michael"},{"last_name":"De Vries","full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"first_name":"Yoshinori","last_name":"Fukui","full_name":"Fukui, Yoshinori"},{"orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","last_name":"Sixt"}],"intvolume":"        26","isi":1,"volume":26,"quality_controlled":"1","abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publication_status":"published","external_id":{"isi":["001529134300001"],"pmid":["40664976"]},"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"date_created":"2025-07-27T22:01:26Z","language":[{"iso":"eng"}],"corr_author":"1","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","day":"01","publisher":"Springer Nature","has_accepted_license":"1","date_published":"2025-08-01T00:00:00Z","project":[{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"},{"_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","grant_number":"944-2020","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration"}],"year":"2025","oa_version":"Published Version","date_updated":"2026-04-28T13:26:50Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"1258–1266","_id":"20082","title":"Migrating immune cells globally coordinate protrusive forces","month":"08","article_processing_charge":"Yes (via OA deal)","citation":{"ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025."},"related_material":{"record":[{"id":"20149","relation":"dissertation_contains","status":"public"}],"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/bench-pressing-cells/","description":"News on ISTA website"}]},"PlanS_conform":"1","ddc":["570"],"OA_place":"publisher","doi":"10.1038/s41590-025-02211-w","OA_type":"hybrid","article_type":"letter_note"},{"has_accepted_license":"1","date_published":"2025-06-12T00:00:00Z","project":[{"_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","grant_number":"26137","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy"},{"call_identifier":"H2020","name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"grant_number":"101044865","_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders"},{"_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","name":"Molecular Drug Targets","call_identifier":"FWF"}],"oa_version":"Published Version","year":"2025","date_updated":"2026-04-28T13:33:34Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"398-410","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publisher":"Springer Nature","day":"12","type":"journal_article","ddc":["570"],"doi":"10.1038/s41586-025-08985-1","OA_place":"publisher","article_type":"original","OA_type":"hybrid","_id":"19704","title":"Light-microscopy-based connectomic reconstruction of mammalian brain tissue","month":"06","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>, vol. 642, Springer Nature, 2025, pp. 398–410, doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>.","short":"M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas, J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino, V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.","ieee":"M. Tavakoli <i>et al.</i>, “Light-microscopy-based connectomic reconstruction of mammalian brain tissue,” <i>Nature</i>, vol. 642. Springer Nature, pp. 398–410, 2025.","apa":"Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas, N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>","ama":"Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. 2025;642:398-410. doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>","ista":"Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 642, 398–410.","chicago":"Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>."},"related_material":{"record":[{"relation":"earlier_version","status":"public","id":"18677"},{"id":"18697","relation":"research_data","status":"public"}],"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/","description":"News on ISTA website"}]},"PlanS_conform":"1","author":[{"first_name":"Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","last_name":"Tavakoli","full_name":"Tavakoli, Mojtaba","orcid":"0000-0002-7667-6854"},{"full_name":"Lyudchik, Julia","last_name":"Lyudchik","first_name":"Julia","id":"46E28B80-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michał","last_name":"Januszewski","full_name":"Januszewski, Michał"},{"last_name":"Vistunou","full_name":"Vistunou, Vitali","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali"},{"id":"40E7F008-F248-11E8-B48F-1D18A9856A87","first_name":"Nathalie","full_name":"Agudelo Duenas, Nathalie","last_name":"Agudelo Duenas"},{"orcid":"0009-0000-7590-3501","last_name":"Vorlaufer","full_name":"Vorlaufer, Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","first_name":"Jakob"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","last_name":"Sommer","first_name":"Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline"},{"full_name":"Oliveira, Bárbara","last_name":"Oliveira","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","first_name":"Bárbara"},{"id":"9ac8f577-2357-11eb-997a-e566c5550886","first_name":"Alban","last_name":"Cenameri","full_name":"Cenameri, Alban"},{"full_name":"Novarino, Gaia","last_name":"Novarino","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","first_name":"Gaia","orcid":"0000-0002-7673-7178"},{"full_name":"Jain, Viren","last_name":"Jain","first_name":"Viren"},{"orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","first_name":"Johann G","full_name":"Danzl, Johann G","last_name":"Danzl"}],"file_date_updated":"2025-07-03T06:55:20Z","scopus_import":"1","publication":"Nature","ec_funded":1,"intvolume":"       642","isi":1,"file":[{"content_type":"application/pdf","checksum":"ebc99d7108e728f46db0a009292675ef","date_updated":"2025-07-03T06:55:20Z","file_name":"2025_Nature_Tavakoli.pdf","creator":"dernst","date_created":"2025-07-03T06:55:20Z","file_id":"19959","file_size":133201290,"access_level":"open_access","relation":"main_file","success":1}],"acknowledgement":"We thank S. Dorkenwald and P. Li for critical reading of the manuscript, S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open access funding provided by Institute of Science and Technology (IST Austria).","pmid":1,"oa":1,"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publication_status":"published","department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"GaNo"}],"external_id":{"isi":["001483477000001"],"pmid":["40335689"]},"date_created":"2025-05-18T22:02:51Z","corr_author":"1","language":[{"iso":"eng"}],"volume":642,"quality_controlled":"1","abstract":[{"text":"The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction by light microscopy has been out of reach, owing to limitations in resolution, contrast and volumetric imaging capability. Here we describe light-microscopy-based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning-based segmentation and analysis of connectivity, thereby directly incorporating molecular information into synapse-level reconstructions of brain tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological experiments in a readily adoptable manner.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"PreCl"},{"_id":"M-Shop"},{"_id":"E-Lib"}]},{"_id":"19626","month":"04","title":"Pulsatile basal gene expression as a fitness determinant in bacteria","article_processing_charge":"Yes (in subscription journal)","related_material":{"record":[{"id":"19294","relation":"research_data","status":"public"}],"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/","relation":"press_release"}]},"citation":{"ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(15). doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>.","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 122(15), e2413709122.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings of the National Academy of Sciences 122 (2025).","mla":"Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15, e2413709122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>.","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Pulsatile basal gene expression as a fitness determinant in bacteria,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15. National Academy of Sciences, 2025."},"ddc":["570"],"article_type":"original","OA_type":"hybrid","doi":"10.1073/pnas.2413709122","OA_place":"publisher","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"issue":"15","publisher":"National Academy of Sciences","day":"15","type":"journal_article","has_accepted_license":"1","date_published":"2025-04-15T00:00:00Z","project":[{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","grant_number":"CZI01","name":"Tools for automation and feedback microscopy"},{"name":"Non-canonical antibiotic interactions","_id":"bd6f94d1-d553-11ed-ba76-ae9f07250f74","grant_number":"E219"},{"grant_number":"I05127","_id":"34e076d6-11ca-11ed-8bc3-aec76c41a181","name":"Evolutionary analysis of gene regulation"}],"date_updated":"2026-04-28T13:35:46Z","oa_version":"Published Version","year":"2025","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","volume":122,"quality_controlled":"1","article_number":"e2413709122","abstract":[{"lang":"eng","text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the noncanonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures."}],"acknowledged_ssus":[{"_id":"Bio"}],"publication_status":"published","date_created":"2025-04-27T22:02:13Z","external_id":{"isi":["001471235200001"],"pmid":["40193613"]},"department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"language":[{"iso":"eng"}],"corr_author":"1","pmid":1,"file":[{"date_created":"2025-06-24T07:27:43Z","file_id":"19888","creator":"dernst","file_name":"2025_PNAS_Jain.pdf","file_size":2949523,"checksum":"115a687f40009660eb4b38b4f6559d41","content_type":"application/pdf","date_updated":"2025-06-24T07:27:43Z","success":1,"relation":"main_file","access_level":"open_access"}],"acknowledgement":"K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions on the manuscript, all other members from the Guet laboratory for valuable feedback, R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic Technology India for allowing time off to address the revisions. K.J. acknowledges Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi), O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.","oa":1,"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"scopus_import":"1","publication":"Proceedings of the National Academy of Sciences","author":[{"last_name":"Jain","full_name":"Jain, Kirti","first_name":"Kirti","id":"330F0278-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3809-0449"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Bochkareva","full_name":"Bochkareva, Olga","first_name":"Olga","id":"C4558D3C-6102-11E9-A62E-F418E6697425","orcid":"0000-0003-1006-6639"},{"orcid":"0000-0001-9480-5261","id":"68E56E44-62B0-11EA-B963-444F3DDC885E","first_name":"Roderich","full_name":"Römhild, Roderich","last_name":"Römhild"},{"orcid":"0000-0002-6699-1455","full_name":"Tkačik, Gašper","last_name":"Tkačik","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","first_name":"Gašper"},{"last_name":"Guet","full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C","orcid":"0000-0001-6220-2052"}],"file_date_updated":"2025-06-24T07:27:43Z","intvolume":"       122","isi":1},{"author":[{"orcid":"0000-0002-3809-0449","id":"330F0278-F248-11E8-B48F-1D18A9856A87","first_name":"Kirti","last_name":"Jain","full_name":"Jain, Kirti"},{"full_name":"Hauschild, 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In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the non-canonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g. control of costly-to-induce multi-drug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multi-drug resistance is crucial for effective public health measures."}],"article_processing_charge":"No","related_material":{"record":[{"status":"public","relation":"used_in_publication","id":"19626"}]},"citation":{"ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>.","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>.","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” 2025. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’” Institute of Science and Technology Austria, 2025.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).","mla":"Jain, Kirti, et al. <i>Data for “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.”</i> Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>."},"_id":"19294","month":"03","title":"Data for \"Pulsatile basal gene expression as a fitness determinant in bacteria\""},{"month":"02","title":"Spontaneous ordering of identical materials into a triboelectric series","_id":"19278","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20203"}],"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/","relation":"press_release"}]},"citation":{"ieee":"J. C. A. Sobarzo Ponce <i>et al.</i>, “Spontaneous ordering of identical materials into a triboelectric series,” <i>Nature</i>, vol. 638, no. 8051. Springer Nature, 2025.","mla":"Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>, vol. 638, no. 8051, 664–669, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>.","short":"J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske, M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature 638 (2025).","ista":"Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering of identical materials into a triboelectric series. Nature. 638(8051), 664–669.","chicago":"Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo, Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>.","apa":"Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske, A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>","ama":"Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. 2025;638(8051). doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>"},"article_processing_charge":"Yes (via OA deal)","ddc":["530"],"article_type":"original","OA_type":"hybrid","OA_place":"publisher","doi":"10.1038/s41586-024-08530-6","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","issue":"8051","publisher":"Springer Nature","type":"journal_article","day":"20","date_updated":"2026-04-28T13:44:56Z","oa_version":"Published Version","year":"2025","date_published":"2025-02-20T00:00:00Z","has_accepted_license":"1","project":[{"_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","grant_number":"949120","call_identifier":"H2020","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","quality_controlled":"1","volume":638,"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"article_number":"664-669","abstract":[{"text":"When two insulating, neutral materials are contacted and separated, they exchange electrical charge1. Experiments have long suggested that this ‘contact electrification’ is transitive, with different materials ordering into ‘triboelectric series’ based on the sign of charge acquired2. At the same time, the effect is plagued by unpredictability, preventing consensus on the mechanism and casting doubt on the rhyme and reason that series imply3. Here we expose an unanticipated connection between the unpredictability and order in contact electrification: nominally identical materials initially exchange charge randomly and intransitively, but—over repeated experiments—order into triboelectric series. We find that this evolution is driven by the act of contact itself—samples with more contacts in their history charge negatively to ones with fewer contacts. Capturing this ‘contact bias’ in a minimal model, we recreate both the initial randomness and ultimate order in numerical simulations and use it experimentally to force the appearance of a triboelectric series of our choosing. With a set of surface-sensitive techniques to search for the underlying alterations contact creates, we only find evidence of nanoscale morphological changes, pointing to a mechanism strongly coupled with mechanics. Our results highlight the centrality of contact history in contact electrification and suggest that focusing on the unpredictability that has long plagued the effect may hold the key to understanding it.","lang":"eng"}],"publication_status":"published","language":[{"iso":"eng"}],"corr_author":"1","date_created":"2025-03-02T23:01:52Z","department":[{"_id":"ScWa"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"external_id":{"pmid":["39972227"],"isi":["001428076100015"]},"oa":1,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"pmid":1,"acknowledgement":"This project has received financing from the European Research Council grant agreement no. 949120 under the European Union’s Horizon 2020 research and innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility, Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez and G. Anciaux for the suggestion to look into the roughness power spectral density. We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements. Open access funding provided by Institute of Science and Technology (IST Austria).","file":[{"access_level":"open_access","relation":"main_file","success":1,"content_type":"application/pdf","checksum":"fecf302274dd3218d3e7dd22f39a6c0c","date_updated":"2025-03-04T10:05:18Z","file_name":"2025_Nature_Sobarzo.pdf","creator":"dernst","file_id":"19289","date_created":"2025-03-04T10:05:18Z","file_size":3807415}],"isi":1,"intvolume":"       638","ec_funded":1,"file_date_updated":"2025-03-04T10:05:18Z","author":[{"first_name":"Juan Carlos A","id":"4B807D68-AE37-11E9-AC72-31CAE5697425","full_name":"Sobarzo Ponce, Juan Carlos A","last_name":"Sobarzo Ponce"},{"last_name":"Pertl","full_name":"Pertl, Felix","first_name":"Felix","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","orcid":"0000-0003-0463-5794"},{"last_name":"Balazs","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","orcid":"0000-0001-7597-043X"},{"orcid":"0000-0001-9732-3815","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","first_name":"Tommaso","full_name":"Costanzo, Tommaso","last_name":"Costanzo"},{"first_name":"Markus","full_name":"Sauer, Markus","last_name":"Sauer"},{"first_name":"Annette","full_name":"Foelske, Annette","last_name":"Foelske"},{"first_name":"Markus","last_name":"Ostermann","full_name":"Ostermann, Markus"},{"first_name":"Christian M.","full_name":"Pichler, Christian M.","last_name":"Pichler"},{"last_name":"Wang","full_name":"Wang, Yongkang","first_name":"Yongkang"},{"first_name":"Yuki","last_name":"Nagata","full_name":"Nagata, Yuki"},{"last_name":"Bonn","full_name":"Bonn, Mischa","first_name":"Mischa"},{"orcid":"0000-0002-2299-3176","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","first_name":"Scott R","full_name":"Waitukaitis, Scott R","last_name":"Waitukaitis"}],"publication":"Nature","scopus_import":"1"},{"volume":60,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues."}],"publication_status":"published","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"external_id":{"pmid":["39603235"],"isi":["001434279000001"]},"date_created":"2025-01-09T11:25:47Z","corr_author":"1","language":[{"iso":"eng"}],"acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","file":[{"success":1,"access_level":"open_access","relation":"main_file","creator":"dernst","file_name":"2025_DevelopmentalCell_Lehr.pdf","file_id":"19584","date_created":"2025-04-16T10:54:07Z","file_size":6994499,"checksum":"bb58db4a908a1f4aabe4004706154541","content_type":"application/pdf","date_updated":"2025-04-16T10:54:07Z"}],"pmid":1,"oa":1,"publication_identifier":{"issn":["1534-5807"]},"file_date_updated":"2025-04-16T10:54:07Z","publication":"Developmental Cell","author":[{"last_name":"Rus","full_name":"Rus, Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","first_name":"Stefanie","orcid":"0000-0001-8703-1093"},{"orcid":"0000-0001-7205-2975","last_name":"Brückner","full_name":"Brückner, David","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d"},{"full_name":"Minchington, Thomas","last_name":"Minchington","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","first_name":"Thomas"},{"first_name":"Martina","id":"48A59534-F248-11E8-B48F-1D18A9856A87","last_name":"Greunz","full_name":"Greunz, Martina"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"orcid":"0000-0003-4509-4998","last_name":"Kicheva","full_name":"Kicheva, Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","first_name":"Anna"}],"scopus_import":"1","isi":1,"intvolume":"        60","_id":"18807","title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","month":"02","article_processing_charge":"Yes (via OA deal)","citation":{"apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. 2025;60(4):567-580. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>.","mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>, vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>.","short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","ieee":"S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>, vol. 60, no. 4. Elsevier, pp. 567–580, 2025."},"related_material":{"record":[{"id":"19763","relation":"dissertation_contains","status":"public"}]},"ddc":["570"],"doi":"10.1016/j.devcel.2024.10.024","OA_place":"publisher","OA_type":"hybrid","article_type":"original","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publisher":"Elsevier","type":"journal_article","day":"24","issue":"4","project":[{"grant_number":"101044579","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","name":"Mechanisms of tissue size regulation in spinal cord development"},{"_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F7802","name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord"},{"_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011","name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube"}],"has_accepted_license":"1","date_published":"2025-02-24T00:00:00Z","year":"2025","oa_version":"Published Version","date_updated":"2026-04-28T22:30:58Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"567-580"},{"ddc":["570"],"article_type":"original","OA_type":"gold","OA_place":"publisher","doi":"10.1038/s41467-024-51754-3","month":"08","title":"The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation","_id":"17885","citation":{"chicago":"Kofler, Lisa, Lorenz Grundmann, Magdalena Gerhalter, Michael Prattes, Juliane Merl-Pham, Gertrude Zisser, Irina Grishkovskaya, et al. “The Novel Ribosome Biogenesis Inhibitor Usnic Acid Blocks Nucleolar Pre-60S Maturation.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-51754-3\">https://doi.org/10.1038/s41467-024-51754-3</a>.","ista":"Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau V-V, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. 2024. The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. Nature Communications. 15, 7511.","ama":"Kofler L, Grundmann L, Gerhalter M, et al. The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-51754-3\">10.1038/s41467-024-51754-3</a>","apa":"Kofler, L., Grundmann, L., Gerhalter, M., Prattes, M., Merl-Pham, J., Zisser, G., … Bergler, H. (2024). The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-51754-3\">https://doi.org/10.1038/s41467-024-51754-3</a>","ieee":"L. Kofler <i>et al.</i>, “The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","short":"L. Kofler, L. Grundmann, M. Gerhalter, M. Prattes, J. Merl-Pham, G. Zisser, I. Grishkovskaya, V.-V. Hodirnau, M. Vareka, R. Breinbauer, S.M. Hauck, D. Haselbach, H. Bergler, Nature Communications 15 (2024).","mla":"Kofler, Lisa, et al. “The Novel Ribosome Biogenesis Inhibitor Usnic Acid Blocks Nucleolar Pre-60S Maturation.” <i>Nature Communications</i>, vol. 15, 7511, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-51754-3\">10.1038/s41467-024-51754-3</a>."},"article_processing_charge":"Yes","date_updated":"2025-09-08T09:13:01Z","year":"2024","oa_version":"Published Version","has_accepted_license":"1","date_published":"2024-08-29T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","day":"29","publisher":"Springer Nature","type":"journal_article","publication_status":"published","language":[{"iso":"eng"}],"date_created":"2024-09-08T22:01:10Z","department":[{"_id":"EM-Fac"}],"external_id":{"pmid":["39209816"],"isi":["001457895200001"]},"quality_controlled":"1","volume":15,"acknowledged_ssus":[{"_id":"EM-Fac"}],"article_number":"7511","abstract":[{"text":"The formation of new ribosomes is tightly coordinated with cell growth and proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and RNAs follows an intricate scheme of maturation and rearrangement steps across three cellular compartments: the nucleolus, nucleoplasm, and cytoplasm. We demonstrate that usnic acid, a lichen secondary metabolite, inhibits the maturation of the large ribosomal subunit in yeast. We combine biochemical characterization of pre-ribosomal particles with a quantitative single-particle cryo-EM approach to monitor changes in nucleolar particle populations upon drug treatment. Usnic acid rapidly blocks the transition from nucleolar state B to C of Nsa1-associated pre-ribosomes, depleting key maturation factors such as Dbp10 and hindering pre-rRNA processing. This primary nucleolar block rapidly rebounds on earlier stages of the pathway which highlights the regulatory linkages between different steps. In summary, we provide an in-depth characterization of the effect of usnic acid on ribosome biogenesis, which may have implications for its reported anti-cancer activities.","lang":"eng"}],"isi":1,"intvolume":"        15","file_date_updated":"2024-09-09T08:56:12Z","publication":"Nature Communications","scopus_import":"1","author":[{"last_name":"Kofler","full_name":"Kofler, Lisa","first_name":"Lisa"},{"full_name":"Grundmann, Lorenz","last_name":"Grundmann","first_name":"Lorenz"},{"first_name":"Magdalena","last_name":"Gerhalter","full_name":"Gerhalter, Magdalena"},{"last_name":"Prattes","full_name":"Prattes, Michael","first_name":"Michael"},{"last_name":"Merl-Pham","full_name":"Merl-Pham, Juliane","first_name":"Juliane"},{"first_name":"Gertrude","full_name":"Zisser, Gertrude","last_name":"Zisser"},{"full_name":"Grishkovskaya, Irina","last_name":"Grishkovskaya","first_name":"Irina"},{"last_name":"Hodirnau","full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X"},{"last_name":"Vareka","full_name":"Vareka, Martin","first_name":"Martin"},{"full_name":"Breinbauer, Rolf","last_name":"Breinbauer","first_name":"Rolf"},{"last_name":"Hauck","full_name":"Hauck, Stefanie M.","first_name":"Stefanie M."},{"last_name":"Haselbach","full_name":"Haselbach, David","first_name":"David"},{"first_name":"Helmut","full_name":"Bergler, Helmut","last_name":"Bergler"}],"publication_identifier":{"eissn":["2041-1723"]},"oa":1,"pmid":1,"acknowledgement":"We thank Michael A. McAlear, Micheline Fromont-Racin, Philipp Milkereit, Arlen W. Johnson, Sabine Rospert, Ed Hurt, C. Yam, Günter Daum, Wolfgang Zachariae, Katrin Karbstein, Juan P. G. Ballesta, Mercedes Dosil, Miguel Remacha und Jesus de la Cruz for sharing strains or providing antibodies. We thank the members of the Bergler lab and the Haselbach lab for their helpful discussion. We thank Ellen Zhong for helpful discussions about the quantitative cryoDRGN analysis. This research was supported by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility. This research was funded in whole, or in part, by the Austrian Science Foundation grants [https://doi.org/10.55776/P32977], [https://doi.org/10.55776/P29451] and [https://doi.org/10.55776/P32536] (to H.B.). Research at the IMP is generously supported by Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter grant FFG-852936). For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","DOAJ_listed":"1","file":[{"file_name":"2024_NatureComm_Kofler.pdf","creator":"dernst","date_created":"2024-09-09T08:56:12Z","file_id":"17946","file_size":3735024,"content_type":"application/pdf","checksum":"7c044538a47182c826d1b526c52958a2","date_updated":"2024-09-09T08:56:12Z","success":1,"access_level":"open_access","relation":"main_file"}]},{"article_processing_charge":"No","citation":{"short":"D. Kleindienst, T. Costanzo, R. Shigemoto, in:, J.H.R. Lübke, A. Rollenhagen (Eds.), New Aspects in Analyzing the Synaptic Organization of the Brain, 1st ed., Springer Nature, New York, 2024, pp. 123–137.","mla":"Kleindienst, David, et al. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., Springer Nature, 2024, pp. 123–37, doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>.","ieee":"D. Kleindienst, T. Costanzo, and R. Shigemoto, “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning,” in <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, 1st ed., J. H. R. Lübke and A. Rollenhagen, Eds. New York: Springer Nature, 2024, pp. 123–137.","ama":"Kleindienst D, Costanzo T, Shigemoto R. Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: Lübke JHR, Rollenhagen A, eds. <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>. 1st ed. New York: Springer Nature; 2024:123-137. doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>","apa":"Kleindienst, D., Costanzo, T., &#38; Shigemoto, R. (2024). Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In J. H. R. Lübke &#38; A. Rollenhagen (Eds.), <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i> (1st ed., pp. 123–137). New York: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>","chicago":"Kleindienst, David, Tommaso Costanzo, and Ryuichi Shigemoto. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” In <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., 123–37. New York: Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>.","ista":"Kleindienst D, Costanzo T, Shigemoto R. 2024.Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: New Aspects in Analyzing the Synaptic Organization of the Brain. Neuromethods, , 123–137."},"_id":"18052","month":"08","title":"Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning","alternative_title":["Neuromethods"],"doi":"10.1007/978-1-0716-4019-7_8","day":"27","type":"book_chapter","publisher":"Springer Nature","status":"public","edition":"1","place":"New York","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"123-137","date_published":"2024-08-27T00:00:00Z","project":[{"_id":"25CA28EA-B435-11E9-9278-68D0E5697425","grant_number":"694539","call_identifier":"H2020","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour"}],"date_updated":"2025-04-14T07:27:15Z","year":"2024","oa_version":"None","abstract":[{"lang":"eng","text":"Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is an electron microscope (EM) sample preparation technique which allows for high-resolution visualization of membrane proteins with high sensitivity. However, image acquisition of specific replica profiles such as synapses in a large field of EM view needs a valid experience and a long time for manual searching. Here, we describe how to utilize deep learning for automatizing image acquisition of specific profiles of interest in replica samples. This protocol facilitates the labor-intensive collection of EM images, in particular for rare profiles. We provide instructions for using SerialEM image acquisition software in conjunction with object detection by our newly developed deep learning software DarEM, to automatically acquire tilt series of all synapses in a selected region. We then show how to perform a mostly automated analysis of gold particle labeling in the acquired images by utilizing Darea software."}],"editor":[{"full_name":"Lübke, Joachim H.R. ","last_name":"Lübke","first_name":"Joachim H.R. "},{"full_name":"Rollenhagen, Astrid","last_name":"Rollenhagen","first_name":"Astrid"}],"acknowledged_ssus":[{"_id":"EM-Fac"}],"quality_controlled":"1","date_created":"2024-09-10T12:32:38Z","department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published","acknowledgement":"This research was supported by the European Research Council Advanced Grant 694539 to RS and by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility.","publication_identifier":{"eisbn":["9781071640197"],"isbn":["9781071640180"],"issn":["0893-2336"],"eissn":["1940-6045"]},"scopus_import":"1","author":[{"full_name":"Kleindienst, David","last_name":"Kleindienst","first_name":"David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9732-3815","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","first_name":"Tommaso","last_name":"Costanzo","full_name":"Costanzo, Tommaso"},{"orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi"}],"publication":"New Aspects in Analyzing the Synaptic Organization of the Brain","ec_funded":1},{"status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publisher":"American Chemical Society","day":"11","type":"journal_article","issue":"3","date_published":"2024-03-11T00:00:00Z","has_accepted_license":"1","year":"2024","oa_version":"Published Version","date_updated":"2025-09-08T09:52:18Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"1759-1774","_id":"18168","title":"Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein","month":"03","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Wu, Dongqing, et al. “Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.” <i>Biomacromolecules</i>, vol. 25, no. 3, American Chemical Society, 2024, pp. 1759–74, doi:<a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">10.1021/acs.biomac.3c01239</a>.","short":"D. Wu, A. Koscic, S. Schneider, R.C.A. Dubini, D.C. Rodriguez Camargo, S. Schneider, P. Rovo, Biomacromolecules 25 (2024) 1759–1774.","ieee":"D. Wu <i>et al.</i>, “Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein,” <i>Biomacromolecules</i>, vol. 25, no. 3. American Chemical Society, pp. 1759–1774, 2024.","ama":"Wu D, Koscic A, Schneider S, et al. Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. <i>Biomacromolecules</i>. 2024;25(3):1759-1774. doi:<a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">10.1021/acs.biomac.3c01239</a>","apa":"Wu, D., Koscic, A., Schneider, S., Dubini, R. C. A., Rodriguez Camargo, D. C., Schneider, S., &#38; Rovo, P. (2024). Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. <i>Biomacromolecules</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">https://doi.org/10.1021/acs.biomac.3c01239</a>","chicago":"Wu, Dongqing, Anamaria Koscic, Sonja Schneider, Romeo C. A. Dubini, Diana C. Rodriguez Camargo, Sabine Schneider, and Petra Rovo. “Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.” <i>Biomacromolecules</i>. American Chemical Society, 2024. <a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">https://doi.org/10.1021/acs.biomac.3c01239</a>.","ista":"Wu D, Koscic A, Schneider S, Dubini RCA, Rodriguez Camargo DC, Schneider S, Rovo P. 2024. Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. Biomacromolecules. 25(3), 1759–1774."},"ddc":["540"],"doi":"10.1021/acs.biomac.3c01239","article_type":"original","acknowledgement":"We thank Dr. Pavel Kielkowski for performing the MS/MS measurement and providing feedback on the manuscript. We are grateful to Rodrigo Ledesma Amaro for introducing the Golden Gate Assembly technique in our lab. We acknowledge the support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)─SFB 1309-325871075, the Center for NanoScience (CeNS), the Fonds der Chemischen Industrie, and Universitätsgesellschaft München.","file":[{"relation":"main_file","access_level":"open_access","success":1,"content_type":"application/pdf","checksum":"9552b6d52f1e8a350764849a535fc13e","date_updated":"2024-10-07T08:33:35Z","date_created":"2024-10-07T08:33:35Z","file_id":"18180","creator":"dernst","file_name":"2024_BioMacromolecules_Wu.pdf","file_size":6597227}],"pmid":1,"oa":1,"publication_identifier":{"issn":["1525-7797"],"eissn":["1526-4602"]},"publication":"Biomacromolecules","file_date_updated":"2024-10-07T08:33:35Z","author":[{"last_name":"Wu","full_name":"Wu, Dongqing","first_name":"Dongqing"},{"full_name":"Koscic, Anamaria","last_name":"Koscic","first_name":"Anamaria"},{"first_name":"Sonja","last_name":"Schneider","full_name":"Schneider, Sonja"},{"first_name":"Romeo C. A.","full_name":"Dubini, Romeo C. A.","last_name":"Dubini"},{"first_name":"Diana C.","full_name":"Rodriguez Camargo, Diana C.","last_name":"Rodriguez Camargo"},{"full_name":"Schneider, Sabine","last_name":"Schneider","first_name":"Sabine"},{"orcid":"0000-0001-8729-7326","id":"c316e53f-b965-11eb-b128-bb26acc59c00","first_name":"Petra","full_name":"Rovo, Petra","last_name":"Rovo"}],"scopus_import":"1","intvolume":"        25","isi":1,"volume":25,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Despite the considerable interest in the recombinant production of synthetic spider silk fibers that possess mechanical properties similar to those of native spider silks, such as the cost-effectiveness, tunability, and scalability realization, is still lacking. To address this long-standing challenge, we have constructed an artificial spider silk gene using Golden Gate assembly for the recombinant bacterial production of dragline-mimicking silk, incorporating all the essential components: the N-terminal domain, a 33-residue-long major-ampullate-spidroin-inspired segment repeated 16 times, and the C-terminal domain (N16C). This designed silk-like protein was successfully expressed in Escherichia coli, purified, and cast into films from formic acid. We produced uniformly 13C–15N-labeled N16C films and employed solid-state magic-angle spinning nuclear magnetic resonance (NMR) for characterization. Thus, we could demonstrate that our bioengineered silk-like protein self-assembles into a film where, when hydrated, the solvent-exposed layer of the rigid, β-nanocrystalline polyalanine core undergoes a transition to an α-helical structure, gaining mobility to the extent that it fully dissolves in water and transforms into a highly dynamic random coil. This hydration-induced behavior induces chain dynamics in the glycine-rich amorphous soft segments on the microsecond time scale, contributing to the elasticity of the solid material. Our findings not only reveal the presence of structurally and dynamically distinct segments within the film’s superstructure but also highlight the complexity of the self-organization responsible for the exceptional mechanical properties observed in proteins that mimic dragline silk."}],"publication_status":"published","external_id":{"isi":["001166501000001"],"pmid":["38343096"]},"department":[{"_id":"NMR"}],"date_created":"2024-10-02T10:09:53Z","corr_author":"1","language":[{"iso":"eng"}]},{"citation":{"chicago":"Kitsara, Maria, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>. SAGE Publications, 2024. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>.","ista":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, Bert B, Bubalo N, Erden S, Franco NH, Chirico G, Gómez Raja J, Gonzalez-Uarquin F, Lang A, Linklater N, Mojsova S, Olsson IAS, Sandvig I, Schaffert A, Schmit M, Schober S, Sevastre B, Wilflingseder D, Ahluwalia A, Neuhaus W. 2024. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to Laboratory Animals. 52(6), 326–333.","ama":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, et al. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. 2024;52(6):326-333. doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>","apa":"Kitsara, M., Smajlhodžić-Deljo, M., Gurbeta Pokvic, L., Bert, B., Bubalo, N., Erden, S., … Neuhaus, W. (2024). Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>","ieee":"M. Kitsara <i>et al.</i>, “Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE),” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6. SAGE Publications, pp. 326–333, 2024.","mla":"Kitsara, Maria, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6, SAGE Publications, 2024, pp. 326–33, doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>.","short":"M. Kitsara, M. Smajlhodžić-Deljo, L. Gurbeta Pokvic, B. Bert, N. Bubalo, S. Erden, N.H. Franco, G. Chirico, J. Gómez Raja, F. Gonzalez-Uarquin, A. Lang, N. Linklater, S. Mojsova, I.A.S. Olsson, I. Sandvig, A. Schaffert, M. Schmit, S. Schober, B. Sevastre, D. Wilflingseder, A. Ahluwalia, W. Neuhaus, Alternatives to Laboratory Animals 52 (2024) 326–333."},"article_processing_charge":"No","title":"Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)","month":"11","_id":"18310","doi":"10.1177/02611929241286024","OA_type":"closed access","article_type":"original","publisher":"SAGE Publications","day":"01","type":"journal_article","issue":"6","status":"public","page":"326-333","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa_version":"None","year":"2024","date_updated":"2025-09-08T09:56:39Z","date_published":"2024-11-01T00:00:00Z","quality_controlled":"1","volume":52,"language":[{"iso":"eng"}],"department":[{"_id":"PreCl"}],"external_id":{"pmid":["39333027"],"isi":["001348633700007"]},"date_created":"2024-10-13T22:01:51Z","publication_status":"published","publication_identifier":{"issn":["0261-1929"],"eissn":["2632-3559"]},"pmid":1,"isi":1,"intvolume":"        52","scopus_import":"1","author":[{"first_name":"Maria","full_name":"Kitsara, Maria","last_name":"Kitsara"},{"first_name":"Merima","last_name":"Smajlhodžić-Deljo","full_name":"Smajlhodžić-Deljo, Merima"},{"first_name":"Lejla","full_name":"Gurbeta Pokvic, Lejla","last_name":"Gurbeta Pokvic"},{"full_name":"Bert, Bettina","last_name":"Bert","first_name":"Bettina"},{"first_name":"Nataliia","last_name":"Bubalo","full_name":"Bubalo, Nataliia"},{"last_name":"Erden","full_name":"Erden, Sevilay","first_name":"Sevilay"},{"first_name":"Nuno Henrique","full_name":"Franco, Nuno Henrique","last_name":"Franco"},{"first_name":"Giuseppe","last_name":"Chirico","full_name":"Chirico, Giuseppe"},{"last_name":"Gómez Raja","full_name":"Gómez Raja, Jonathan","first_name":"Jonathan"},{"first_name":"Fernando","last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando"},{"first_name":"Annemarie","full_name":"Lang, Annemarie","last_name":"Lang"},{"last_name":"Linklater","full_name":"Linklater, Nicole","first_name":"Nicole"},{"last_name":"Mojsova","full_name":"Mojsova, Sandra","first_name":"Sandra"},{"last_name":"Olsson","full_name":"Olsson, I. Anna S.","first_name":"I. Anna S."},{"first_name":"Ioanna","full_name":"Sandvig, Ioanna","last_name":"Sandvig"},{"full_name":"Schaffert, Alexandra","last_name":"Schaffert","first_name":"Alexandra"},{"last_name":"Schmit","full_name":"Schmit, Marthe","first_name":"Marthe"},{"first_name":"Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","full_name":"Schober, Sophie","last_name":"Schober"},{"full_name":"Sevastre, Bogdan","last_name":"Sevastre","first_name":"Bogdan"},{"first_name":"Doris","full_name":"Wilflingseder, Doris","last_name":"Wilflingseder"},{"first_name":"Arti","last_name":"Ahluwalia","full_name":"Ahluwalia, Arti"},{"full_name":"Neuhaus, Winfried","last_name":"Neuhaus","first_name":"Winfried"}],"publication":"Alternatives to Laboratory Animals"},{"status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"type":"journal_article","day":"01","publisher":"Springer Nature","has_accepted_license":"1","date_published":"2024-12-01T00:00:00Z","date_updated":"2025-09-09T11:41:12Z","oa_version":"Published Version","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"18581","month":"12","title":"Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium","article_processing_charge":"Yes","citation":{"apa":"Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek, A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. Springer Nature. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>","ama":"Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. 2024;15. doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>","ista":"Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research and Therapy. 15, 434.","chicago":"Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin, Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>.","short":"D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek, L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl, W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).","mla":"Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>, vol. 15, 434, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>.","ieee":"D. Marolt Presen <i>et al.</i>, “Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium,” <i>Stem Cell Research and Therapy</i>, vol. 15. Springer Nature, 2024."},"ddc":["570"],"OA_type":"gold","article_type":"original","doi":"10.1186/s13287-024-03960-5","OA_place":"publisher","pmid":1,"file":[{"success":1,"access_level":"open_access","relation":"main_file","file_size":6690494,"file_name":"2024_StemCellResearch_Presen.pdf","creator":"dernst","date_created":"2024-12-10T08:28:17Z","file_id":"18641","date_updated":"2024-12-10T08:28:17Z","checksum":"91edba8edde30d781dce89fdd5cadc39","content_type":"application/pdf"}],"acknowledgement":"We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for providing the human tissue waste for primary cell isolation and the New York Stem Cell Foundation Research Institute for providing the human induced pluripotent stem cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing support of the project. InstaText writing tool (https://instatext.io) was used to edit the English language of the final manuscript.\r\nThis work has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and 853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951) and the Production of the Future program (grant agreement No. 877452).","DOAJ_listed":"1","oa":1,"publication_identifier":{"eissn":["1757-6512"]},"publication":"Stem Cell Research and Therapy","file_date_updated":"2024-12-10T08:28:17Z","author":[{"full_name":"Marolt Presen, Darja","last_name":"Marolt Presen","first_name":"Darja"},{"first_name":"Vanessa","full_name":"Goeschl, Vanessa","last_name":"Goeschl"},{"full_name":"Hanetseder, Dominik","last_name":"Hanetseder","first_name":"Dominik"},{"first_name":"Laura","full_name":"Ogrin, Laura","last_name":"Ogrin"},{"last_name":"Stetco","full_name":"Stetco, Alexandra Larissa","first_name":"Alexandra Larissa"},{"last_name":"Tansek","full_name":"Tansek, Anja","first_name":"Anja"},{"first_name":"Laura","last_name":"Pozenel","full_name":"Pozenel, Laura"},{"id":"70abbbb3-88ea-11ec-8e0a-e8c939944834","first_name":"Bella","last_name":"Bruszel","full_name":"Bruszel, Bella"},{"last_name":"Mitulovic","full_name":"Mitulovic, Goran","first_name":"Goran"},{"first_name":"Johannes","full_name":"Oesterreicher, Johannes","last_name":"Oesterreicher"},{"full_name":"Zipperle, Johannes","last_name":"Zipperle","first_name":"Johannes"},{"full_name":"Schaedl, Barbara","last_name":"Schaedl","first_name":"Barbara"},{"full_name":"Holnthoner, Wolfgang","last_name":"Holnthoner","first_name":"Wolfgang"},{"first_name":"Johannes","full_name":"Grillari, Johannes","last_name":"Grillari"},{"full_name":"Redl, Heinz","last_name":"Redl","first_name":"Heinz"}],"scopus_import":"1","isi":1,"intvolume":"        15","volume":15,"quality_controlled":"1","article_number":"434","abstract":[{"text":"Background: Human induced pluripotent stem cells represent a scalable source of youthful tissue progenitors and secretomes for regenerative therapies. The aim of our study was to investigate the potential of conditioned medium (CM) from hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13 donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures and the effects on proliferation and osteogenic differentiation were examined after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared with the activity of MSC-derived CM and with the activity of CM prepared from hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative proteomic analysis of CM was performed to gain insight into the molecular components responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline phosphatase activity of MSC from several adult/aged donors after 14 days of continuous supplementation under osteogenic conditions. However, CM supplementation failed to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions. hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity. Quantitative proteomic analysis of d14-CM revealed a distinct profile of components that formed a highly interconnected associations network with two clusters, one functionally associated with binding and organization of actin/cytoskeletal components and the other with structural constituents of the extracellular matrix, collagen, and growth factor binding. Several hub proteins were identified that were reported to have functions in cell-extracellular matrix interaction, osteogenic differentiation and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early osteogenic differentiation of human bone marrow-derived MSCs and that prolonged cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis of the upregulated protein components provides the basis for further optimization of hiPSC-MP-CM for bone regenerative therapies.","lang":"eng"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","publication_status":"published","date_created":"2024-11-24T23:01:47Z","department":[{"_id":"LifeSc"}],"external_id":{"isi":["001356479400001"],"pmid":["39551765"]},"language":[{"iso":"eng"}]},{"abstract":[{"lang":"eng","text":"Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts."}],"volume":53,"quality_controlled":"1","external_id":{"isi":["001355264100001"],"pmid":["39548348"]},"department":[{"_id":"PreCl"}],"date_created":"2024-11-24T23:01:49Z","language":[{"iso":"eng"}],"publication_status":"published","acknowledgement":"This publication is based upon work from the Ethics Crossover Group within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology). We acknowledge the input and advice from Dr. Susanna Louhimies.","file":[{"checksum":"67fc140f761581a291591f075e49b88d","content_type":"application/pdf","date_updated":"2024-12-03T14:07:04Z","file_name":"2024_LabAnimal_Lauwereyns.pdf","creator":"dernst","file_id":"18614","date_created":"2024-12-03T14:07:04Z","file_size":967252,"access_level":"open_access","relation":"main_file","success":1}],"pmid":1,"oa":1,"publication_identifier":{"issn":["0093-7355"],"eissn":["1548-4475"]},"author":[{"first_name":"Jan","full_name":"Lauwereyns, Jan","last_name":"Lauwereyns"},{"full_name":"Bajramovic, Jeffrey","last_name":"Bajramovic","first_name":"Jeffrey"},{"last_name":"Bert","full_name":"Bert, Bettina","first_name":"Bettina"},{"first_name":"Samuel","full_name":"Camenzind, Samuel","last_name":"Camenzind"},{"last_name":"De Kock","full_name":"De Kock, Joery","first_name":"Joery"},{"first_name":"Alisa","last_name":"Elezović","full_name":"Elezović, Alisa"},{"full_name":"Erden, Sevilay","last_name":"Erden","first_name":"Sevilay"},{"first_name":"Fernando","last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando"},{"last_name":"Ulman","full_name":"Ulman, Yesim Isil","first_name":"Yesim Isil"},{"first_name":"Orsolya Ivett","last_name":"Hoffmann","full_name":"Hoffmann, Orsolya Ivett"},{"first_name":"Maria","full_name":"Kitsara, Maria","last_name":"Kitsara"},{"last_name":"Kostomitsopoulos","full_name":"Kostomitsopoulos, Nikolaos","first_name":"Nikolaos"},{"first_name":"Winfried","last_name":"Neuhaus","full_name":"Neuhaus, Winfried"},{"last_name":"Petit-Demouliere","full_name":"Petit-Demouliere, Benoit","first_name":"Benoit"},{"full_name":"Pollo, Simone","last_name":"Pollo","first_name":"Simone"},{"last_name":"Riso","full_name":"Riso, Brígida","first_name":"Brígida"},{"id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","first_name":"Sophie","last_name":"Schober","full_name":"Schober, Sophie"},{"full_name":"Sotiropoulos, Athanassia","last_name":"Sotiropoulos","first_name":"Athanassia"},{"first_name":"Aurélie","full_name":"Thomas, Aurélie","last_name":"Thomas"},{"last_name":"Vitale","full_name":"Vitale, Augusto","first_name":"Augusto"},{"last_name":"Wilflingseder","full_name":"Wilflingseder, Doris","first_name":"Doris"},{"first_name":"Arti","full_name":"Ahluwalia, Arti","last_name":"Ahluwalia"}],"scopus_import":"1","publication":"Lab Animal","file_date_updated":"2024-12-03T14:07:04Z","isi":1,"intvolume":"        53","article_processing_charge":"No","citation":{"ieee":"J. Lauwereyns <i>et al.</i>, “Toward a common interpretation of the 3Rs principles in animal research,” <i>Lab Animal</i>, vol. 53. Springer Nature, pp. 347–350, 2024.","mla":"Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>, vol. 53, Springer Nature, 2024, pp. 347–50, doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>.","short":"J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović, S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos, W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos, A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.","ista":"Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs principles in animal research. Lab Animal. 53, 347–350.","chicago":"Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>.","apa":"Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović, A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>","ama":"Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. 2024;53:347-350. doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>"},"_id":"18587","title":"Toward a common interpretation of the 3Rs principles in animal research","month":"12","doi":"10.1038/s41684-024-01476-2","OA_place":"publisher","OA_type":"hybrid","article_type":"letter_note","ddc":["570"],"type":"journal_article","day":"01","publisher":"Springer Nature","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"347-350","has_accepted_license":"1","date_published":"2024-12-01T00:00:00Z","year":"2024","oa_version":"Published Version","date_updated":"2025-09-08T14:50:31Z"},{"language":[{"iso":"eng"}],"external_id":{"pmid":["38598627"]},"department":[{"_id":"EM-Fac"}],"date_created":"2025-01-27T14:32:34Z","publication_status":"published","abstract":[{"lang":"eng","text":"The globally distributed marine alga Emiliania huxleyi has cooling effect on the Earth’s climate. The population density of E. huxleyi is restricted by Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite the impact of E. huxleyi viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from E. huxleyi cells, which enables the continuous release of virions by exocytosis."}],"article_number":"eadk1954 ","quality_controlled":"1","volume":10,"intvolume":"        10","file_date_updated":"2025-01-27T14:40:08Z","publication":"Science Advances","scopus_import":"1","author":[{"first_name":"Miroslav","last_name":"Homola","full_name":"Homola, Miroslav"},{"full_name":"Büttner, Renate Carina","last_name":"Büttner","first_name":"Renate Carina","id":"3b7984c9-17ff-11ed-b6fe-f943c4a5b626"},{"first_name":"Tibor","full_name":"Füzik, Tibor","last_name":"Füzik"},{"full_name":"Křepelka, Pavel","last_name":"Křepelka","first_name":"Pavel"},{"full_name":"Holbová, Radka","last_name":"Holbová","first_name":"Radka"},{"first_name":"Jiří","full_name":"Nováček, Jiří","last_name":"Nováček"},{"full_name":"Chaillet, Marten L.","last_name":"Chaillet","first_name":"Marten L."},{"full_name":"Žák, Jakub","last_name":"Žák","first_name":"Jakub"},{"first_name":"Danyil","full_name":"Grybchuk, Danyil","last_name":"Grybchuk"},{"first_name":"Friedrich","last_name":"Förster","full_name":"Förster, Friedrich"},{"full_name":"Wilson, William H.","last_name":"Wilson","first_name":"William H."},{"last_name":"Schroeder","full_name":"Schroeder, Declan C.","first_name":"Declan C."},{"first_name":"Pavel","last_name":"Plevka","full_name":"Plevka, Pavel"}],"publication_identifier":{"eissn":["2375-2548"]},"oa":1,"file":[{"success":1,"relation":"main_file","access_level":"open_access","file_id":"18921","date_created":"2025-01-27T14:40:08Z","file_name":"2024_ScienceAdv_Homola.pdf","creator":"dernst","file_size":40623405,"content_type":"application/pdf","checksum":"291dd7ceccbe6bfd8e0a9157584f88e9","date_updated":"2025-01-27T14:40:08Z"}],"DOAJ_listed":"1","acknowledgement":"We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core Facility and Proteomics Core Facility of the Central European Institute of Technology (CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR); and (iii) Plant Sciences Core Facility for support with obtaining scientific data presented here. We acknowledge support from the project National Institute of Virology and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European Union - Next Generation EU. This work received funding from the Czech Science Foundation grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI” (no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship funded by Brno city municipality to M.H.","pmid":1,"doi":"10.1126/sciadv.adk1954","OA_place":"publisher","OA_type":"gold","article_type":"original","ddc":["570"],"citation":{"mla":"Homola, Miroslav, et al. “Structure and Replication Cycle of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>, vol. 10, no. 15, eadk1954, American Association for the Advancement of Science, 2024, doi:<a href=\"https://doi.org/10.1126/sciadv.adk1954\">10.1126/sciadv.adk1954</a>.","short":"M. Homola, R.C. Büttner, T. Füzik, P. Křepelka, R. Holbová, J. Nováček, M.L. Chaillet, J. Žák, D. Grybchuk, F. Förster, W.H. Wilson, D.C. Schroeder, P. Plevka, Science Advances 10 (2024).","ieee":"M. Homola <i>et al.</i>, “Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi,” <i>Science Advances</i>, vol. 10, no. 15. American Association for the Advancement of Science, 2024.","apa":"Homola, M., Büttner, R. C., Füzik, T., Křepelka, P., Holbová, R., Nováček, J., … Plevka, P. (2024). Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.adk1954\">https://doi.org/10.1126/sciadv.adk1954</a>","ama":"Homola M, Büttner RC, Füzik T, et al. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>. 2024;10(15). doi:<a href=\"https://doi.org/10.1126/sciadv.adk1954\">10.1126/sciadv.adk1954</a>","ista":"Homola M, Büttner RC, Füzik T, Křepelka P, Holbová R, Nováček J, Chaillet ML, Žák J, Grybchuk D, Förster F, Wilson WH, Schroeder DC, Plevka P. 2024. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. Science Advances. 10(15), eadk1954.","chicago":"Homola, Miroslav, Renate Carina Büttner, Tibor Füzik, Pavel Křepelka, Radka Holbová, Jiří Nováček, Marten L. Chaillet, et al. “Structure and Replication Cycle of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>. American Association for the Advancement of Science, 2024. <a href=\"https://doi.org/10.1126/sciadv.adk1954\">https://doi.org/10.1126/sciadv.adk1954</a>."},"related_material":{"link":[{"url":" https://github.com/fuzikt/tomostarpy.","relation":"software"}]},"article_processing_charge":"Yes","title":"Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi","month":"04","_id":"18920","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2024","oa_version":"Published Version","date_updated":"2025-05-14T09:29:04Z","date_published":"2024-04-01T00:00:00Z","has_accepted_license":"1","type":"journal_article","publisher":"American Association for the Advancement of Science","day":"01","issue":"15","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"David","id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic","full_name":"Vijatovic, David"},{"full_name":"Toma, Florina Alexandra ","last_name":"Toma","first_name":"Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e"},{"id":"a8144562-32c9-11ee-b5ce-d9800628bda2","first_name":"Zoe P","last_name":"Harrington","full_name":"Harrington, Zoe P","orcid":"0009-0008-0158-4032"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","full_name":"Sommer, Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J.","last_name":"Trevisan"},{"first_name":"Phillip","full_name":"Chapman, Phillip","last_name":"Chapman"},{"first_name":"Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","last_name":"Julseth","full_name":"Julseth, Mara"},{"first_name":"Susan","full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton"},{"last_name":"Gabitto","full_name":"Gabitto, Mariano I.","first_name":"Mariano I."},{"first_name":"Jeremy S.","full_name":"Dasen, Jeremy S.","last_name":"Dasen"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B.","first_name":"Jay B."},{"orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney"}],"publication":"bioRxiv","date_published":"2024-09-27T00:00:00Z","project":[{"grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"date_updated":"2025-05-14T11:40:13Z","year":"2024","oa_version":"Preprint","day":"27","main_file_link":[{"url":"https://doi.org/10.1101/2024.09.20.614050","open_access":"1"}],"type":"preprint","acknowledgement":"We would like to thank the members of the Sweeney Lab (especially Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion, and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their scientific insight and mentorship during the conception of this project. This\r\nproject would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA). In addition, we thank our funding sources for providing the resources to do these\r\nexperiments: FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS 5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.); NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities (ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472 (Z.H.); and Project A.L.S. (S.B-M.). ","status":"public","oa":1,"date_created":"2025-04-07T08:48:28Z","department":[{"_id":"LoSw"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"OA_type":"green","corr_author":"1","doi":"10.1101/2024.09.20.614050","language":[{"iso":"eng"}],"OA_place":"repository","publication_status":"submitted","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Vertebrates exhibit a wide range of motor behaviors, ranging from swimming to complex limb-based movements. Here we take advantage of frog metamorphosis, which captures a swim-to-limb-based movement transformation during the development of a single organism, to explore changes in the underlying spinal circuits. We find that the tadpole spinal cord contains small and largely homogeneous populations of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages. These neuronal populations only modestly increase in number and subtype heterogeneity with the emergence of free swimming. In contrast, during frog metamorphosis and the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron number and transcriptional heterogeneity, culminating in cohorts of neurons that exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively, results in severe but selective deficits in tail and limb function. Our work thus demonstrates that neural diversity scales exponentially with increasing behavioral complexity and illustrates striking evolutionary conservation in the molecular organization and function of motor circuits across species."}],"acknowledged_ssus":[{"_id":"Bio"}],"citation":{"ama":"Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>","apa":"Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R., Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>","chicago":"Vijatovic, David, Florina Alexandra  Toma, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>.","ista":"Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv, <a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","mla":"Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","short":"D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).","ieee":"D. Vijatovic <i>et al.</i>, “Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis,” <i>bioRxiv</i>. ."},"_id":"19520","month":"09","title":"Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis"},{"file_date_updated":"2024-01-16T10:53:31Z","author":[{"orcid":"0000-0001-5809-9566","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","first_name":"Feyza N","full_name":"Arslan, Feyza N","last_name":"Arslan"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","last_name":"Loose","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"publication":"Current Biology","scopus_import":"1","ec_funded":1,"isi":1,"intvolume":"        34","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","file":[{"date_updated":"2024-01-16T10:53:31Z","checksum":"51220b76d72a614208f84bdbfbaf9b72","content_type":"application/pdf","file_size":5183861,"date_created":"2024-01-16T10:53:31Z","file_id":"14813","creator":"dernst","file_name":"2024_CurrentBiology_Arslan.pdf","relation":"main_file","access_level":"open_access","success":1}],"pmid":1,"publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"oa":1,"publication_status":"published","external_id":{"pmid":["38134934"],"isi":["001154500400001"]},"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"date_created":"2024-01-14T23:00:56Z","language":[{"iso":"eng"}],"corr_author":"1","volume":34,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"has_accepted_license":"1","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"date_published":"2024-01-08T00:00:00Z","oa_version":"Published Version","year":"2024","date_updated":"2025-09-04T11:39:10Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"171-182.e8","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","publisher":"Elsevier","day":"08","issue":"1","ddc":["570"],"doi":"10.1016/j.cub.2023.11.067","article_type":"original","_id":"14795","title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","month":"01","article_processing_charge":"Yes (via OA deal)","citation":{"short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>","chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>.","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8."}},{"volume":20,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"publication_status":"published","date_created":"2024-01-21T23:00:57Z","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"external_id":{"isi":["001138880800005"],"pmid":["38370025"]},"corr_author":"1","language":[{"iso":"eng"}],"pmid":1,"acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","file":[{"access_level":"open_access","relation":"main_file","success":1,"date_updated":"2024-07-16T12:12:43Z","content_type":"application/pdf","checksum":"7891ebe7c900ae47469ab127031dd1ec","file_size":9897883,"file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","creator":"dernst","date_created":"2024-07-16T12:12:43Z","file_id":"17267"}],"publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"oa":1,"author":[{"id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia","last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia","orcid":"0000-0002-5223-3346"},{"first_name":"Rushikesh","full_name":"Shinde, Rushikesh","last_name":"Shinde"},{"last_name":"Bolger-Munro","full_name":"Bolger-Munro, Madison","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","first_name":"Madison","orcid":"0000-0002-8176-4824"},{"last_name":"Peruzzo","full_name":"Peruzzo, Matilda","first_name":"Matilda","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3415-4628"},{"id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory","full_name":"Szep, Gregory","last_name":"Szep"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","full_name":"Steccari, Irene","last_name":"Steccari"},{"id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","first_name":"David","full_name":"Labrousse Arias, David","last_name":"Labrousse Arias"},{"orcid":"0000-0002-9438-4783","last_name":"Zheden","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack"},{"last_name":"Callan-Jones","full_name":"Callan-Jones, Andrew","first_name":"Andrew"},{"last_name":"Voituriez","full_name":"Voituriez, Raphaël","first_name":"Raphaël"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"publication":"Nature Physics","scopus_import":"1","file_date_updated":"2024-07-16T12:12:43Z","isi":1,"intvolume":"        20","_id":"14846","month":"02","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","article_processing_charge":"Yes (in subscription journal)","related_material":{"link":[{"url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","relation":"press_release","description":"News on ISTA Website"}]},"citation":{"apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024."},"ddc":["530"],"article_type":"original","doi":"10.1038/s41567-023-02302-1","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"day":"01","publisher":"Springer Nature","type":"journal_article","date_published":"2024-02-01T00:00:00Z","has_accepted_license":"1","project":[{"_id":"2646861A-B435-11E9-9278-68D0E5697425","grant_number":"I03601","call_identifier":"FWF","name":"Control of embryonic cleavage pattern"}],"date_updated":"2025-09-04T11:48:28Z","oa_version":"Published Version","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"310-321"},{"license":"https://opensource.org/licenses/MIT","citation":{"short":"R. Hauschild, (2024).","mla":"Hauschild, Robert. <i>Matlab Script for Analysis of Clone Dispersal</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14926\">10.15479/AT:ISTA:14926</a>.","ieee":"R. Hauschild, “Matlab script for analysis of clone dispersal.” Institute of Science and Technology Austria, 2024.","ama":"Hauschild R. Matlab script for analysis of clone dispersal. 2024. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14926\">10.15479/AT:ISTA:14926</a>","apa":"Hauschild, R. (2024). Matlab script for analysis of clone dispersal. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:14926\">https://doi.org/10.15479/AT:ISTA:14926</a>","chicago":"Hauschild, Robert. “Matlab Script for Analysis of Clone Dispersal.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/AT:ISTA:14926\">https://doi.org/10.15479/AT:ISTA:14926</a>.","ista":"Hauschild R. 2024. Matlab script for analysis of clone dispersal, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:14926\">10.15479/AT:ISTA:14926</a>."},"related_material":{"record":[{"id":"15048","relation":"used_in_publication","status":"public"}]},"_id":"14926","title":"Matlab script for analysis of clone dispersal","month":"02","department":[{"_id":"Bio"}],"date_created":"2024-02-02T14:42:26Z","doi":"10.15479/AT:ISTA:14926","corr_author":"1","ddc":["570"],"type":"software","day":"02","publisher":"Institute of Science and Technology Austria","file":[{"date_updated":"2024-02-02T14:40:31Z","checksum":"df7f358ae19a176cf710c0a802ce31b1","content_type":"application/octet-stream","file_size":736,"file_name":"README.md","creator":"rhauschild","date_created":"2024-02-02T14:40:31Z","file_id":"14927","access_level":"open_access","relation":"main_file","success":1},{"file_size":3543,"file_id":"14928","date_created":"2024-02-02T14:40:31Z","file_name":"Supplementary_file_1.zip","creator":"rhauschild","date_updated":"2024-02-02T14:40:31Z","content_type":"application/x-zip-compressed","checksum":"10194cc11619eccd8f4b24472e465b7f","success":1,"relation":"main_file","access_level":"open_access"}],"status":"public","oa":1,"tmp":{"name":"The MIT License","legal_code_url":"https://opensource.org/licenses/MIT","short":"MIT"},"author":[{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"}],"file_date_updated":"2024-02-02T14:40:31Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2024-02-02T00:00:00Z","has_accepted_license":"1","year":"2024","date_updated":"2025-09-04T12:10:39Z"},{"file_date_updated":"2024-07-22T11:56:08Z","publication":"Materials Science in Semiconductor Processing","keyword":["Mechanical Engineering","Mechanics of Materials","Condensed Matter Physics","General Materials Science"],"scopus_import":"1","author":[{"first_name":"Yosuke","full_name":"Shimura, Yosuke","last_name":"Shimura"},{"last_name":"Godfrin","full_name":"Godfrin, Clement","first_name":"Clement"},{"first_name":"Andriy","last_name":"Hikavyy","full_name":"Hikavyy, Andriy"},{"full_name":"Li, Roy","last_name":"Li","first_name":"Roy"},{"last_name":"Aguilera Servin","full_name":"Aguilera Servin, Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87","first_name":"Juan L","orcid":"0000-0002-2862-8372"},{"first_name":"Georgios","id":"38DB5788-F248-11E8-B48F-1D18A9856A87","full_name":"Katsaros, Georgios","last_name":"Katsaros","orcid":"0000-0001-8342-202X"},{"full_name":"Favia, Paola","last_name":"Favia","first_name":"Paola"},{"first_name":"Han","last_name":"Han","full_name":"Han, Han"},{"first_name":"Danny","full_name":"Wan, Danny","last_name":"Wan"},{"last_name":"de Greve","full_name":"de Greve, Kristiaan","first_name":"Kristiaan"},{"first_name":"Roger","full_name":"Loo, Roger","last_name":"Loo"}],"intvolume":"       174","isi":1,"acknowledgement":"The Ge project received funding from the European Union's Horizon Europe programme under the Grant Agreement 101069515 – IGNITE. Siltronic AG is acknowledged for providing the SRB wafers. This work was supported by Imec's Industrial Affiliation Program on Quantum Computing.","file":[{"success":1,"relation":"main_file","access_level":"open_access","file_size":4220165,"file_id":"17312","date_created":"2024-07-22T11:56:08Z","file_name":"2024_MaterialsScience_Shimura.pdf","creator":"dernst","date_updated":"2024-07-22T11:56:08Z","checksum":"62e8e9ae960387a3dca32ec7f5e413ab","content_type":"application/pdf"}],"publication_identifier":{"issn":["1369-8001"]},"oa":1,"date_created":"2024-02-22T14:10:40Z","department":[{"_id":"GeKa"},{"_id":"NanoFab"}],"external_id":{"isi":["001188520000001"]},"language":[{"iso":"eng"}],"publication_status":"published","article_number":"108231","abstract":[{"lang":"eng","text":"The epitaxial growth of a strained Ge layer, which is a promising candidate for the channel material of a hole spin qubit, has been demonstrated on 300 mm Si wafers using commercially available Si0.3Ge0.7 strain relaxed buffer (SRB) layers. The assessment of the layer and the interface qualities for a buried strained Ge layer embedded in Si0.3Ge0.7 layers is reported. The XRD reciprocal space mapping confirmed that the reduction of the growth temperature enables the 2-dimensional growth of the Ge layer fully strained with respect to the Si0.3Ge0.7. Nevertheless, dislocations at the top and/or bottom interface of the Ge layer were observed by means of electron channeling contrast imaging, suggesting the importance of the careful dislocation assessment. The interface abruptness does not depend on the selection of the precursor gases, but it is strongly influenced by the growth temperature which affects the coverage of the surface H-passivation. The mobility of 2.7 × 105 cm2/Vs is promising, while the low percolation density of 3 × 1010 /cm2 measured with a Hall-bar device at 7 K illustrates the high quality of the heterostructure thanks to the high Si0.3Ge0.7 SRB quality."}],"volume":174,"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","date_published":"2024-05-20T00:00:00Z","project":[{"grant_number":"101069515","_id":"34c0acea-11ca-11ed-8bc3-8775e10fd452","name":"Integrated Germanium Quantum Technology"}],"date_updated":"2025-04-14T08:01:27Z","year":"2024","oa_version":"Published Version","issue":"5","type":"journal_article","day":"20","publisher":"Elsevier","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"OA_type":"hybrid","article_type":"original","OA_place":"publisher","doi":"10.1016/j.mssp.2024.108231","ddc":["530"],"article_processing_charge":"Yes (in subscription journal)","citation":{"mla":"Shimura, Yosuke, et al. “Compressively Strained Epitaxial Ge Layers for Quantum Computing Applications.” <i>Materials Science in Semiconductor Processing</i>, vol. 174, no. 5, 108231, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">10.1016/j.mssp.2024.108231</a>.","short":"Y. Shimura, C. Godfrin, A. Hikavyy, R. Li, J.L. Aguilera Servin, G. Katsaros, P. Favia, H. Han, D. Wan, K. de Greve, R. Loo, Materials Science in Semiconductor Processing 174 (2024).","ieee":"Y. Shimura <i>et al.</i>, “Compressively strained epitaxial Ge layers for quantum computing applications,” <i>Materials Science in Semiconductor Processing</i>, vol. 174, no. 5. Elsevier, 2024.","ama":"Shimura Y, Godfrin C, Hikavyy A, et al. Compressively strained epitaxial Ge layers for quantum computing applications. <i>Materials Science in Semiconductor Processing</i>. 2024;174(5). doi:<a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">10.1016/j.mssp.2024.108231</a>","apa":"Shimura, Y., Godfrin, C., Hikavyy, A., Li, R., Aguilera Servin, J. L., Katsaros, G., … Loo, R. (2024). Compressively strained epitaxial Ge layers for quantum computing applications. <i>Materials Science in Semiconductor Processing</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">https://doi.org/10.1016/j.mssp.2024.108231</a>","chicago":"Shimura, Yosuke, Clement Godfrin, Andriy Hikavyy, Roy Li, Juan L Aguilera Servin, Georgios Katsaros, Paola Favia, et al. “Compressively Strained Epitaxial Ge Layers for Quantum Computing Applications.” <i>Materials Science in Semiconductor Processing</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.mssp.2024.108231\">https://doi.org/10.1016/j.mssp.2024.108231</a>.","ista":"Shimura Y, Godfrin C, Hikavyy A, Li R, Aguilera Servin JL, Katsaros G, Favia P, Han H, Wan D, de Greve K, Loo R. 2024. Compressively strained epitaxial Ge layers for quantum computing applications. Materials Science in Semiconductor Processing. 174(5), 108231."},"_id":"15018","month":"05","title":"Compressively strained epitaxial Ge layers for quantum computing applications"}]