[{"issue":"2","type":"journal_article","day":"01","publisher":"VÖB","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"200 - 207","has_accepted_license":"1","date_published":"2017-08-01T00:00:00Z","date_updated":"2025-07-10T11:55:09Z","year":"2017","oa_version":"Published Version","article_processing_charge":"No","citation":{"apa":"Petritsch, B. (2017). Metadata for research data in practice. <i>Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. VÖB. <a href=\"https://doi.org/10.31263/voebm.v70i2.1678\">https://doi.org/10.31263/voebm.v70i2.1678</a>","ama":"Petritsch B. Metadata for research data in practice. <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. 2017;70(2):200-207. doi:<a href=\"https://doi.org/10.31263/voebm.v70i2.1678\">10.31263/voebm.v70i2.1678</a>","ista":"Petritsch B. 2017. Metadata for research data in practice. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare. 70(2), 200–207.","chicago":"Petritsch, Barbara. “Metadata for Research Data in Practice.” <i>Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. VÖB, 2017. <a href=\"https://doi.org/10.31263/voebm.v70i2.1678\">https://doi.org/10.31263/voebm.v70i2.1678</a>.","mla":"Petritsch, Barbara. “Metadata for Research Data in Practice.” <i>Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>, vol. 70, no. 2, VÖB, 2017, pp. 200–07, doi:<a href=\"https://doi.org/10.31263/voebm.v70i2.1678\">10.31263/voebm.v70i2.1678</a>.","short":"B. Petritsch, Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare 70 (2017) 200–207.","ieee":"B. Petritsch, “Metadata for research data in practice,” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>, vol. 70, no. 2. VÖB, pp. 200–207, 2017."},"_id":"825","month":"08","title":"Metadata for research data in practice","publist_id":"6823","doi":"10.31263/voebm.v70i2.1678","ddc":["020"],"file":[{"access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:48:11Z","content_type":"application/pdf","checksum":"7c4544d07efa2c2add8612b489abb4e2","file_size":7843975,"file_name":"2017_VOEB_Petritsch.pdf","creator":"dernst","date_created":"2019-01-18T13:32:17Z","file_id":"5850"}],"oa":1,"publication_identifier":{"issn":["1022-2588"]},"scopus_import":"1","author":[{"orcid":"0000-0003-2724-4614","last_name":"Petritsch","full_name":"Petritsch, Barbara","id":"406048EC-F248-11E8-B48F-1D18A9856A87","first_name":"Barbara"}],"file_date_updated":"2020-07-14T12:48:11Z","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare","intvolume":"        70","abstract":[{"lang":"eng","text":"What data is needed about data? Describing the process to answer this question for the institutional data repository IST DataRep."}],"license":"https://creativecommons.org/licenses/by/4.0/","volume":70,"date_created":"2018-12-11T11:48:42Z","department":[{"_id":"E-Lib"}],"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published"},{"publication_status":"published","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"external_id":{"isi":["000404728300001"]},"date_created":"2018-12-11T11:49:21Z","language":[{"iso":"eng"}],"volume":6,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes."}],"article_number":"e26792","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"publication":"eLife","scopus_import":"1","file_date_updated":"2020-07-14T12:48:15Z","author":[{"orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Von Wangenheim","full_name":"Von Wangenheim, Daniel"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699"},{"orcid":"0000-0003-2676-3367","first_name":"Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","last_name":"Barone"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"ec_funded":1,"isi":1,"intvolume":"         6","acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","file":[{"access_level":"open_access","relation":"main_file","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","creator":"system","date_created":"2018-12-12T10:17:57Z","file_id":"5315","file_size":19581847,"checksum":"9af3398cb0d81f99d79016a616df22e9","content_type":"application/pdf","date_updated":"2020-07-14T12:48:15Z"}],"oa":1,"ddc":["570"],"pubrep_id":"847","publist_id":"6471","doi":"10.7554/eLife.26792","_id":"946","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","month":"06","article_processing_charge":"Yes","citation":{"ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>.","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017."},"related_material":{"record":[{"relation":"popular_science","status":"public","id":"5566"}]},"has_accepted_license":"1","project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"grant_number":"M02128","_id":"2572ED28-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular basis of root growth inhibition by auxin"},{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development"},{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"date_published":"2017-06-19T00:00:00Z","oa_version":"Published Version","year":"2017","date_updated":"2025-04-15T06:37:26Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publisher":"eLife Sciences Publications","type":"journal_article","day":"19"},{"publication_status":"published","pubrep_id":"724","ddc":["020"],"language":[{"iso":"eng"}],"corr_author":"1","OA_place":"publisher","OA_type":"gold","department":[{"_id":"E-Lib"}],"date_created":"2018-12-12T11:39:24Z","title":"Implementing the institutional data repository IST DataRep","month":"06","_id":"5450","citation":{"ista":"Petritsch B. 2017. Implementing the institutional data repository IST DataRep, Institute of Science and Technology Austria,p.","chicago":"Petritsch, Barbara. <i>Implementing the Institutional Data Repository IST DataRep</i>. Institute of Science and Technology Austria, 2017.","apa":"Petritsch, B. (2017). <i>Implementing the institutional data repository IST DataRep</i>. Institute of Science and Technology Austria.","ama":"Petritsch B. <i>Implementing the Institutional Data Repository IST DataRep</i>. Institute of Science and Technology Austria; 2017.","ieee":"B. Petritsch, <i>Implementing the institutional data repository IST DataRep</i>. Institute of Science and Technology Austria, 2017.","mla":"Petritsch, Barbara. <i>Implementing the Institutional Data Repository IST DataRep</i>. Institute of Science and Technology Austria, 2017.","short":"B. Petritsch, Implementing the Institutional Data Repository IST DataRep, Institute of Science and Technology Austria, 2017."},"abstract":[{"text":"In this report the implementation of the institutional data repository IST DataRep at IST Austria will be covered: Starting with the research phase when requirements for a repository were established, the procedure of choosing a repository-software and its customization based on the results of user-testings will be discussed. Followed by reflections on the marketing strategies in regard of impact, and at the end sharing some experiences of one year operating IST DataRep.","lang":"eng"}],"article_processing_charge":"No","year":"2017","oa_version":"Published Version","date_updated":"2025-07-10T11:52:50Z","date_published":"2017-06-26T00:00:00Z","has_accepted_license":"1","file_date_updated":"2020-07-14T12:46:59Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"406048EC-F248-11E8-B48F-1D18A9856A87","first_name":"Barbara","full_name":"Petritsch, Barbara","last_name":"Petritsch","orcid":"0000-0003-2724-4614"}],"oa":1,"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","file":[{"checksum":"6321792dcfa82bf490f17615a9b22355","content_type":"application/pdf","date_updated":"2020-07-14T12:46:59Z","date_created":"2018-12-12T11:53:22Z","file_id":"5483","creator":"system","file_name":"IST-2017-724-v1+1_DataRep_Project_Report_2017.pdf","file_size":3460985,"relation":"main_file","access_level":"open_access"}],"type":"report","day":"26","publisher":"Institute of Science and Technology Austria"},{"ddc":["571"],"doi":"10.15479/AT:ISTA:53","date_created":"2018-12-12T12:31:32Z","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"Bio"}],"month":"03","title":"Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity","datarep_id":"53","_id":"5560","related_material":{"record":[{"id":"665","relation":"research_paper","status":"public"}]},"citation":{"ieee":"T. Bergmiller <i>et al.</i>, “Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity.” Institute of Science and Technology Austria, 2017.","short":"T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild, G. Tkačik, C.C. Guet, (2017).","mla":"Bergmiller, Tobias, et al. <i>Biased Partitioning of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:53\">10.15479/AT:ISTA:53</a>.","chicago":"Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza, Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:53\">https://doi.org/10.15479/AT:ISTA:53</a>.","ista":"Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik G, Guet CC. 2017. Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:53\">10.15479/AT:ISTA:53</a>.","ama":"Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:53\">10.15479/AT:ISTA:53</a>","apa":"Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild, R., … Guet, C. C. (2017). Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:53\">https://doi.org/10.15479/AT:ISTA:53</a>"},"abstract":[{"text":"This repository contains the data collected for the manuscript \"Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity\".\r\nThe data is compressed into a single archive. Within the archive, different folders correspond to figures of the main text and the SI of the related publication.\r\nData is saved as plain text, with each folder containing a separate readme file describing the format. Typically, the data is from fluorescence microscopy measurements of single cells growing in a microfluidic \"mother machine\" device, and consists of relevant values (primarily arbitrary unit or normalized fluorescence measurements, and division times / growth rates) after raw microscopy images have been processed, segmented, and their features extracted, as described in the methods section of the related publication.","lang":"eng"}],"license":"https://creativecommons.org/publicdomain/zero/1.0/","article_processing_charge":"No","date_updated":"2025-09-11T07:05:03Z","oa_version":"Published Version","year":"2017","date_published":"2017-03-10T00:00:00Z","has_accepted_license":"1","keyword":["single cell microscopy","mother machine microfluidic device","AcrAB-TolC pump","multi-drug efflux","Escherichia coli"],"author":[{"orcid":"0000-0001-5396-4346","first_name":"Tobias","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","last_name":"Bergmiller","full_name":"Bergmiller, Tobias"},{"full_name":"Andersson, Anna M","last_name":"Andersson","first_name":"Anna M","id":"2B8A40DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2912-6769"},{"id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","first_name":"Kathrin","last_name":"Tomasek","full_name":"Tomasek, Kathrin","orcid":"0000-0003-3768-877X"},{"last_name":"Balleza","full_name":"Balleza, Enrique","first_name":"Enrique"},{"first_name":"Daniel","last_name":"Kiviet","full_name":"Kiviet, Daniel"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-6699-1455","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","first_name":"Gasper","last_name":"Tkacik","full_name":"Tkacik, Gasper"},{"orcid":"0000-0001-6220-2052","last_name":"Guet","full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2020-07-14T12:47:03Z","tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","short":"CC0 (1.0)","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)"},"oa":1,"file":[{"date_updated":"2020-07-14T12:47:03Z","content_type":"application/zip","checksum":"d77859af757ac8025c50c7b12b52eaf3","file_size":6773204,"file_name":"IST-2017-53-v1+1_Data_MDE.zip","creator":"system","file_id":"5603","date_created":"2018-12-12T13:02:38Z","access_level":"open_access","relation":"main_file"}],"status":"public","publisher":"Institute of Science and Technology Austria","type":"research_data","day":"10"},{"related_material":{"record":[{"id":"1078","relation":"research_paper","status":"public"}]},"citation":{"ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017.","mla":"von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","short":"D. von Wangenheim, R. Hauschild, J. Friml, (2017).","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>.","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","ama":"von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>"},"abstract":[{"text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. \r\nThe Video is licensed under a CC BY NC ND license. ","lang":"eng"}],"article_processing_charge":"No","month":"04","datarep_id":"66","title":"Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel","_id":"5565","doi":"10.15479/AT:ISTA:66","publist_id":"6302","date_created":"2018-12-12T12:31:34Z","department":[{"_id":"JiFr"},{"_id":"Bio"}],"ddc":["580"],"publisher":"Institute of Science and Technology Austria","type":"research_data","day":"10","oa":1,"acknowledgement":"fund: FP7-ERC 0101109","status":"public","file":[{"access_level":"open_access","relation":"main_file","file_size":101497758,"file_name":"IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4","creator":"system","file_id":"5599","date_created":"2018-12-12T13:02:33Z","date_updated":"2020-07-14T12:47:03Z","checksum":"b7552fc23540a85dc5a22fd4484eae71","content_type":"video/mp4"}],"ec_funded":1,"file_date_updated":"2020-07-14T12:47:03Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","orcid":"0000-0002-6862-1247"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"date_updated":"2025-04-15T07:48:04Z","oa_version":"Published Version","year":"2017","has_accepted_license":"1","project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"date_published":"2017-04-10T00:00:00Z"},{"citation":{"ama":"Hauschild R. Live tracking of moving samples in confocal microscopy for vertically grown roots. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:69\">10.15479/AT:ISTA:69</a>","apa":"Hauschild, R. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:69\">https://doi.org/10.15479/AT:ISTA:69</a>","chicago":"Hauschild, Robert. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:69\">https://doi.org/10.15479/AT:ISTA:69</a>.","ista":"Hauschild R. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:69\">10.15479/AT:ISTA:69</a>.","mla":"Hauschild, Robert. <i>Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:69\">10.15479/AT:ISTA:69</a>.","short":"R. Hauschild, (2017).","ieee":"R. Hauschild, “Live tracking of moving samples in confocal microscopy for vertically grown roots.” Institute of Science and Technology Austria, 2017."},"related_material":{"record":[{"id":"946","relation":"research_paper","status":"public"}]},"license":"https://creativecommons.org/licenses/by-sa/4.0/","article_processing_charge":"No","abstract":[{"text":"Current minimal version of TipTracker","lang":"eng"}],"title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","datarep_id":"69","month":"07","_id":"5566","doi":"10.15479/AT:ISTA:69","department":[{"_id":"Bio"}],"date_created":"2018-12-12T12:31:34Z","ddc":["570"],"type":"research_data","day":"21","publisher":"Institute of Science and Technology Austria","oa":1,"tmp":{"short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png"},"status":"public","file":[{"relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:47:04Z","checksum":"a976000e6715106724a271cc9422be4a","content_type":"application/zip","file_size":1587986,"date_created":"2018-12-12T13:04:12Z","file_id":"5636","file_name":"IST-2017-69-v1+2_TipTrackerZeissLSM700.zip","creator":"system"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2020-07-14T12:47:04Z","author":[{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"}],"keyword":["tool","tracking","confocal microscopy"],"year":"2017","oa_version":"Published Version","date_updated":"2025-04-15T07:48:05Z","date_published":"2017-07-21T00:00:00Z","has_accepted_license":"1"},{"date_published":"2017-10-04T00:00:00Z","has_accepted_license":"1","oa_version":"Published Version","year":"2017","date_updated":"2024-02-21T13:47:14Z","file_date_updated":"2020-07-14T12:47:04Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","keyword":["Cell migration","tracking","forward migration index","FMI"],"author":[{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"}],"file":[{"access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:47:04Z","content_type":"application/octet-stream","checksum":"cb7a2fa622460eca6231d659ce590e32","file_size":799,"file_name":"IST-2017-75-v1+1_FMI.m","creator":"system","date_created":"2018-12-12T13:02:29Z","file_id":"5596"}],"status":"public","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","short":"CC0 (1.0)","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)"},"type":"research_data","publisher":"Institute of Science and Technology Austria","day":"04","ddc":["570"],"department":[{"_id":"Bio"}],"date_created":"2018-12-12T12:31:35Z","doi":"10.15479/AT:ISTA:75","_id":"5570","title":"Forward migration indexes","datarep_id":"75","month":"10","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Matlab script to calculate the forward migration indexes (<d_y>/<L>) from TrackMate spot-statistics files."}],"citation":{"ieee":"R. Hauschild, “Forward migration indexes.” Institute of Science and Technology Austria, 2017.","mla":"Hauschild, Robert. <i>Forward Migration Indexes</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:75\">10.15479/AT:ISTA:75</a>.","short":"R. Hauschild, (2017).","ista":"Hauschild R. 2017. Forward migration indexes, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:75\">10.15479/AT:ISTA:75</a>.","chicago":"Hauschild, Robert. “Forward Migration Indexes.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:75\">https://doi.org/10.15479/AT:ISTA:75</a>.","apa":"Hauschild, R. (2017). Forward migration indexes. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:75\">https://doi.org/10.15479/AT:ISTA:75</a>","ama":"Hauschild R. Forward migration indexes. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:75\">10.15479/AT:ISTA:75</a>"}},{"language":[{"iso":"eng"}],"department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"external_id":{"isi":["000426828000047"]},"date_created":"2018-12-11T11:47:36Z","publication_status":"published","abstract":[{"lang":"eng","text":"Background: Standards have become available to share semantically encoded vital parameters from medical devices, as required for example by personal healthcare records. Standardised sharing of biosignal data largely remains open. Objectives: The goal of this work is to explore available biosignal file format and data exchange standards and profiles, and to conceptualise end-To-end solutions. Methods: The authors reviewed and discussed available biosignal file format standards with other members of international standards development organisations (SDOs). Results: A raw concept for standards based acquisition, storage, archiving and sharing of biosignals was developed. The GDF format may serve for storing biosignals. Signals can then be shared using FHIR resources and may be stored on FHIR servers or in DICOM archives, with DICOM waveforms as one possible format. Conclusion: Currently a group of international SDOs (e.g. HL7, IHE, DICOM, IEEE) is engaged in intensive discussions. This discussion extends existing work that already was adopted by large implementer communities. The concept presented here only reports the current status of the discussion in Austria. The discussion will continue internationally, with results to be expected over the coming years."}],"license":"https://creativecommons.org/licenses/by-nc/4.0/","quality_controlled":"1","volume":236,"intvolume":"       236","isi":1,"file_date_updated":"2020-07-14T12:47:27Z","scopus_import":"1","author":[{"first_name":"Stefan","full_name":"Sauermann, Stefan","last_name":"Sauermann"},{"first_name":"Veronika","last_name":"David","full_name":"David, Veronika"},{"orcid":"0000-0002-5621-8100","last_name":"Schlögl","full_name":"Schlögl, Alois","first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Reinhard","full_name":"Egelkraut, Reinhard","last_name":"Egelkraut"},{"last_name":"Frohner","full_name":"Frohner, Matthias","first_name":"Matthias"},{"last_name":"Pohn","full_name":"Pohn, Birgit","first_name":"Birgit"},{"last_name":"Urbauer","full_name":"Urbauer, Philipp","first_name":"Philipp"},{"full_name":"Mense, Alexander","last_name":"Mense","first_name":"Alexander"}],"publication_identifier":{"isbn":["978-161499758-0"]},"oa":1,"file":[{"content_type":"application/pdf","checksum":"1254dcc5b04a996d97fad9a726b42727","date_updated":"2020-07-14T12:47:27Z","file_name":"IST-2017-906-v1+1_SHTI236-0356.pdf","creator":"system","date_created":"2018-12-12T10:11:56Z","file_id":"4913","file_size":443635,"access_level":"open_access","relation":"main_file"}],"publist_id":"7164","doi":"10.3233/978-1-61499-759-7-356","alternative_title":["Studies in Health Technology and Informatics"],"pubrep_id":"906","ddc":["005"],"citation":{"ieee":"S. Sauermann <i>et al.</i>, “Biosignals standards and FHIR: The way to go,” presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria, 2017, vol. 236, pp. 356–362.","short":"S. Sauermann, V. David, A. Schlögl, R. Egelkraut, M. Frohner, B. Pohn, P. Urbauer, A. Mense, in:, IOS Press, 2017, pp. 356–362.","mla":"Sauermann, Stefan, et al. <i>Biosignals Standards and FHIR: The Way to Go</i>. Vol. 236, IOS Press, 2017, pp. 356–62, doi:<a href=\"https://doi.org/10.3233/978-1-61499-759-7-356\">10.3233/978-1-61499-759-7-356</a>.","chicago":"Sauermann, Stefan, Veronika David, Alois Schlögl, Reinhard Egelkraut, Matthias Frohner, Birgit Pohn, Philipp Urbauer, and Alexander Mense. “Biosignals Standards and FHIR: The Way to Go,” 236:356–62. IOS Press, 2017. <a href=\"https://doi.org/10.3233/978-1-61499-759-7-356\">https://doi.org/10.3233/978-1-61499-759-7-356</a>.","ista":"Sauermann S, David V, Schlögl A, Egelkraut R, Frohner M, Pohn B, Urbauer P, Mense A. 2017. Biosignals standards and FHIR: The way to go. eHealth: Health Informatics Meets eHealth, Studies in Health Technology and Informatics, vol. 236, 356–362.","ama":"Sauermann S, David V, Schlögl A, et al. Biosignals standards and FHIR: The way to go. In: Vol 236. IOS Press; 2017:356-362. doi:<a href=\"https://doi.org/10.3233/978-1-61499-759-7-356\">10.3233/978-1-61499-759-7-356</a>","apa":"Sauermann, S., David, V., Schlögl, A., Egelkraut, R., Frohner, M., Pohn, B., … Mense, A. (2017). Biosignals standards and FHIR: The way to go (Vol. 236, pp. 356–362). Presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria: IOS Press. <a href=\"https://doi.org/10.3233/978-1-61499-759-7-356\">https://doi.org/10.3233/978-1-61499-759-7-356</a>"},"article_processing_charge":"No","conference":{"name":"eHealth: Health Informatics Meets eHealth","start_date":"2017-05-23","location":"Vienna, Austria","end_date":"2017-05-24"},"title":"Biosignals standards and FHIR: The way to go","month":"01","_id":"630","page":"356 - 362","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2017","oa_version":"Published Version","date_updated":"2025-09-11T07:27:53Z","date_published":"2017-01-01T00:00:00Z","has_accepted_license":"1","type":"conference","publisher":"IOS Press","day":"01","tmp":{"short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"status":"public"},{"language":[{"iso":"eng"}],"corr_author":"1","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"Bio"}],"external_id":{"isi":["000399540100060"]},"date_created":"2018-12-11T11:47:48Z","publication_status":"published","abstract":[{"lang":"eng","text":"The molecular mechanisms underlying phenotypic variation in isogenic bacterial populations remain poorly understood.We report that AcrAB-TolC, the main multidrug efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex formation. Mother cells inheriting old poles are phenotypically distinct and display increased drug efflux activity relative to daughters. Consequently, we find systematic and long-lived growth differences between mother and daughter cells in the presence of subinhibitory drug concentrations. A simple model for biased partitioning predicts a population structure of long-lived and highly heterogeneous phenotypes. This straightforward mechanism of generating sustained growth rate differences at subinhibitory antibiotic concentrations has implications for understanding the emergence of multidrug resistance in bacteria."}],"quality_controlled":"1","volume":356,"isi":1,"intvolume":"       356","publication":"Science","author":[{"full_name":"Bergmiller, Tobias","last_name":"Bergmiller","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","first_name":"Tobias","orcid":"0000-0001-5396-4346"},{"first_name":"Anna M","id":"2B8A40DA-F248-11E8-B48F-1D18A9856A87","last_name":"Andersson","full_name":"Andersson, Anna M","orcid":"0000-0003-2912-6769"},{"full_name":"Tomasek, Kathrin","last_name":"Tomasek","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","first_name":"Kathrin","orcid":"0000-0003-3768-877X"},{"full_name":"Balleza, Enrique","last_name":"Balleza","first_name":"Enrique"},{"last_name":"Kiviet","full_name":"Kiviet, Daniel","first_name":"Daniel"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"orcid":"0000-0002-6699-1455","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","first_name":"Gasper","last_name":"Tkacik","full_name":"Tkacik, Gasper"},{"first_name":"Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C","last_name":"Guet","orcid":"0000-0001-6220-2052"}],"scopus_import":"1","publication_identifier":{"issn":["0036-8075"]},"doi":"10.1126/science.aaf4762","publist_id":"7064","article_type":"original","citation":{"chicago":"Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza, Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” <i>Science</i>. American Association for the Advancement of Science, 2017. <a href=\"https://doi.org/10.1126/science.aaf4762\">https://doi.org/10.1126/science.aaf4762</a>.","ista":"Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik G, Guet CC. 2017. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. 356(6335), 311–315.","ama":"Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. <i>Science</i>. 2017;356(6335):311-315. doi:<a href=\"https://doi.org/10.1126/science.aaf4762\">10.1126/science.aaf4762</a>","apa":"Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild, R., … Guet, C. C. (2017). Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aaf4762\">https://doi.org/10.1126/science.aaf4762</a>","ieee":"T. Bergmiller <i>et al.</i>, “Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity,” <i>Science</i>, vol. 356, no. 6335. American Association for the Advancement of Science, pp. 311–315, 2017.","short":"T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild, G. Tkačik, C.C. Guet, Science 356 (2017) 311–315.","mla":"Bergmiller, Tobias, et al. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” <i>Science</i>, vol. 356, no. 6335, American Association for the Advancement of Science, 2017, pp. 311–15, doi:<a href=\"https://doi.org/10.1126/science.aaf4762\">10.1126/science.aaf4762</a>."},"related_material":{"record":[{"relation":"popular_science","status":"public","id":"5560"}]},"article_processing_charge":"No","title":"Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity","month":"04","_id":"665","page":"311 - 315","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa_version":"None","year":"2017","date_updated":"2025-09-11T07:05:04Z","date_published":"2017-04-21T00:00:00Z","project":[{"grant_number":"P28844-B27","_id":"254E9036-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Biophysics of information processing in gene regulation"}],"day":"21","type":"journal_article","publisher":"American Association for the Advancement of Science","issue":"6335","status":"public"},{"publication_status":"published","date_created":"2018-12-11T11:47:50Z","external_id":{"isi":["000402124100002"]},"department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"corr_author":"1","language":[{"iso":"eng"}],"volume":19,"quality_controlled":"1","abstract":[{"text":"Trafficking cells frequently transmigrate through epithelial and endothelial monolayers. How monolayers cooperate with the penetrating cells to support their transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic capillaries as a model system for transendothelial migration. We find that the chemokine CCL21, which is the decisive guidance cue for intravasation, mainly localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes extracellularly enriched at the sites of endothelial cell-cell junctions. When we reconstitute the transmigration process in vitro, we find that secretion of CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and selective calcium chelation in lymphatic endothelium attenuates transmigration. Altogether, our data demonstrate a chemokine-mediated feedback between DCs and lymphatic endothelium, which facilitates transendothelial migration.","lang":"eng"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","file_date_updated":"2020-07-14T12:47:38Z","publication":"Cell Reports","scopus_import":"1","author":[{"last_name":"Vaahtomeri","full_name":"Vaahtomeri, Kari","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518"},{"id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","first_name":"Markus","last_name":"Brown","full_name":"Brown, Markus"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid","last_name":"De Vries"},{"orcid":"0000-0002-1073-744X","last_name":"Leithner","full_name":"Leithner, Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F"},{"orcid":"0000-0001-8599-1226","full_name":"Mehling, Matthias","last_name":"Mehling","first_name":"Matthias","id":"3C23B994-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","full_name":"Kaufmann, Walter","last_name":"Kaufmann"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K"}],"intvolume":"        19","isi":1,"ec_funded":1,"file":[{"relation":"main_file","access_level":"open_access","content_type":"application/pdf","checksum":"8fdddaab1f1d76a6ec9ca94dcb6b07a2","date_updated":"2020-07-14T12:47:38Z","file_id":"5109","date_created":"2018-12-12T10:14:54Z","file_name":"IST-2017-900-v1+1_1-s2.0-S2211124717305211-main.pdf","creator":"system","file_size":2248814}],"publication_identifier":{"issn":["2211-1247"]},"oa":1,"ddc":["570"],"pubrep_id":"900","doi":"10.1016/j.celrep.2017.04.027","publist_id":"7052","_id":"672","month":"05","title":"Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia","article_processing_charge":"Yes","citation":{"ieee":"K. Vaahtomeri <i>et al.</i>, “Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia,” <i>Cell Reports</i>, vol. 19, no. 5. Cell Press, pp. 902–909, 2017.","mla":"Vaahtomeri, Kari, et al. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” <i>Cell Reports</i>, vol. 19, no. 5, Cell Press, 2017, pp. 902–09, doi:<a href=\"https://doi.org/10.1016/j.celrep.2017.04.027\">10.1016/j.celrep.2017.04.027</a>.","short":"K. Vaahtomeri, M. Brown, R. Hauschild, I. de Vries, A.F. Leithner, M. Mehling, W. Kaufmann, M.K. Sixt, Cell Reports 19 (2017) 902–909.","ista":"Vaahtomeri K, Brown M, Hauschild R, de Vries I, Leithner AF, Mehling M, Kaufmann W, Sixt MK. 2017. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. 19(5), 902–909.","chicago":"Vaahtomeri, Kari, Markus Brown, Robert Hauschild, Ingrid de Vries, Alexander F Leithner, Matthias Mehling, Walter Kaufmann, and Michael K Sixt. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” <i>Cell Reports</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.celrep.2017.04.027\">https://doi.org/10.1016/j.celrep.2017.04.027</a>.","apa":"Vaahtomeri, K., Brown, M., Hauschild, R., de Vries, I., Leithner, A. F., Mehling, M., … Sixt, M. K. (2017). Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2017.04.027\">https://doi.org/10.1016/j.celrep.2017.04.027</a>","ama":"Vaahtomeri K, Brown M, Hauschild R, et al. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. <i>Cell Reports</i>. 2017;19(5):902-909. doi:<a href=\"https://doi.org/10.1016/j.celrep.2017.04.027\">10.1016/j.celrep.2017.04.027</a>"},"has_accepted_license":"1","date_published":"2017-05-02T00:00:00Z","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Cytoskeletal force generation and force transduction of migrating leukocytes"}],"date_updated":"2025-09-10T14:27:34Z","year":"2017","oa_version":"Published Version","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"902 - 909","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"issue":"5","type":"journal_article","day":"02","publisher":"Cell Press"},{"_id":"674","title":"Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6","month":"05","article_processing_charge":"No","citation":{"chicago":"Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current Biology</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">https://doi.org/10.1016/j.cub.2017.04.004</a>.","ista":"Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.","ama":"Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current Biology</i>. 2017;27(9):1314-1325. doi:<a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">10.1016/j.cub.2017.04.004</a>","apa":"Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries, I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">https://doi.org/10.1016/j.cub.2017.04.004</a>","ieee":"J. Schwarz <i>et al.</i>, “Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6,” <i>Current Biology</i>, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017.","short":"J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries, A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt, Current Biology 27 (2017) 1314–1325.","mla":"Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current Biology</i>, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:<a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">10.1016/j.cub.2017.04.004</a>."},"doi":"10.1016/j.cub.2017.04.004","publist_id":"7050","status":"public","publisher":"Cell Press","day":"09","type":"journal_article","issue":"9","project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FWF"}],"date_published":"2017-05-09T00:00:00Z","oa_version":"None","year":"2017","date_updated":"2025-09-10T14:26:47Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"1314 - 1325","volume":27,"quality_controlled":"1","abstract":[{"lang":"eng","text":"Navigation of cells along gradients of guidance cues is a determining step in many developmental and immunological processes. Gradients can either be soluble or immobilized to tissues as demonstrated for the haptotactic migration of dendritic cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate how gradient characteristics govern cellular response patterns, we here introduce an in vitro system allowing to track migratory responses of DCs to precisely controlled immobilized gradients of CCL21. We find that haptotactic sensing depends on the absolute CCL21 concentration and local steepness of the gradient, consistent with a scenario where DC directionality is governed by the signal-to-noise ratio of CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore, we find that CCR7 signal termination by the G-protein-coupled receptor kinase 6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient sensing in vitro and confirm those observations in vivo. These findings suggest that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal guidance in vivo."}],"publication_status":"published","external_id":{"isi":["000400741700021"]},"department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"date_created":"2018-12-11T11:47:51Z","corr_author":"1","language":[{"iso":"eng"}],"publication_identifier":{"issn":["09609822"]},"scopus_import":"1","author":[{"full_name":"Schwarz, Jan","last_name":"Schwarz","first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"id":"3FD04378-F248-11E8-B48F-1D18A9856A87","first_name":"Veronika","full_name":"Bierbaum, Veronika","last_name":"Bierbaum"},{"first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"full_name":"Brown, Markus","last_name":"Brown","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","first_name":"Markus"},{"full_name":"De Vries, Ingrid","last_name":"De Vries","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Leithner","full_name":"Leithner, Alexander F","first_name":"Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X"},{"id":"35B76592-F248-11E8-B48F-1D18A9856A87","first_name":"Anne","full_name":"Reversat, Anne","last_name":"Reversat","orcid":"0000-0003-0666-8928"},{"full_name":"Merrin, Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","orcid":"0000-0001-5145-4609"},{"full_name":"Tarrant, Teresa","last_name":"Tarrant","first_name":"Teresa"},{"orcid":"0000-0003-4398-476X","full_name":"Bollenbach, Tobias","last_name":"Bollenbach","first_name":"Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"publication":"Current Biology","ec_funded":1,"intvolume":"        27","isi":1},{"issue":"10","day":"15","publisher":"Optica Publishing Group","type":"journal_article","status":"public","page":"1931 - 1934","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-10T14:25:19Z","year":"2017","oa_version":"None","date_published":"2017-05-15T00:00:00Z","citation":{"ieee":"J. Haase, S. Bagiante, H. Sigg, and J. Van Bokhoven, “Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas,” <i>Optics Letters</i>, vol. 42, no. 10. Optica Publishing Group, pp. 1931–1934, 2017.","mla":"Haase, Johannes, et al. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” <i>Optics Letters</i>, vol. 42, no. 10, Optica Publishing Group, 2017, pp. 1931–34, doi:<a href=\"https://doi.org/10.1364/OL.42.001931\">10.1364/OL.42.001931</a>.","short":"J. Haase, S. Bagiante, H. Sigg, J. Van Bokhoven, Optics Letters 42 (2017) 1931–1934.","chicago":"Haase, Johannes, Salvatore Bagiante, Hans Sigg, and Jeroen Van Bokhoven. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” <i>Optics Letters</i>. Optica Publishing Group, 2017. <a href=\"https://doi.org/10.1364/OL.42.001931\">https://doi.org/10.1364/OL.42.001931</a>.","ista":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. 2017. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics Letters. 42(10), 1931–1934.","ama":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. <i>Optics Letters</i>. 2017;42(10):1931-1934. doi:<a href=\"https://doi.org/10.1364/OL.42.001931\">10.1364/OL.42.001931</a>","apa":"Haase, J., Bagiante, S., Sigg, H., &#38; Van Bokhoven, J. (2017). Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. <i>Optics Letters</i>. Optica Publishing Group. <a href=\"https://doi.org/10.1364/OL.42.001931\">https://doi.org/10.1364/OL.42.001931</a>"},"article_processing_charge":"No","month":"05","title":"Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas","_id":"675","article_type":"original","publist_id":"7048","doi":"10.1364/OL.42.001931","ddc":["530"],"intvolume":"        42","isi":1,"author":[{"first_name":"Johannes","full_name":"Haase, Johannes","last_name":"Haase"},{"orcid":"0000-0002-0122-9603","first_name":"Salvatore","id":"38ED402E-F248-11E8-B48F-1D18A9856A87","last_name":"Bagiante","full_name":"Bagiante, Salvatore"},{"last_name":"Sigg","full_name":"Sigg, Hans","first_name":"Hans"},{"first_name":"Jeroen","full_name":"Van Bokhoven, Jeroen","last_name":"Van Bokhoven"}],"publication":"Optics Letters","scopus_import":"1","abstract":[{"text":"We report the enhancement of infrared absorption of chemisorbed carbon monoxide on platinum in the gap of plasmonic nanoantennas. Our method is based on the self-assembled formation of platinum nanoislands on nanoscopic dipole antenna arrays manufactured via electron beam lithography. We employ systematic variations of the plasmonic antenna resonance to precisely couple to the molecular stretch vibration of carbon monoxide adsorbed on the platinum nanoislands. Ultimately, we reach more than 1500-fold infrared absorption enhancements, allowing for an ultrasensitive detection of a monolayer of chemisorbed carbon monoxide. The developed procedure can be adapted to other metal adsorbents and molecular species and could be utilized for coverage sensing in surface catalytic reactions. ","lang":"eng"}],"quality_controlled":"1","volume":42,"language":[{"iso":"eng"}],"date_created":"2018-12-11T11:47:51Z","external_id":{"isi":["000401424900016"]},"department":[{"_id":"NanoFab"}],"publication_status":"published"},{"publication":"PNAS","file_date_updated":"2020-07-14T12:47:44Z","scopus_import":"1","author":[{"last_name":"Miki","full_name":"Miki, Takafumi","first_name":"Takafumi"},{"orcid":"0000-0001-9735-5315","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","full_name":"Kaufmann, Walter"},{"last_name":"Malagon","full_name":"Malagon, Gerardo","first_name":"Gerardo"},{"last_name":"Gomez","full_name":"Gomez, Laura","first_name":"Laura"},{"full_name":"Tabuchi, Katsuhiko","last_name":"Tabuchi","first_name":"Katsuhiko"},{"first_name":"Masahiko","full_name":"Watanabe, Masahiko","last_name":"Watanabe"},{"orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi"},{"last_name":"Marty","full_name":"Marty, Alain","first_name":"Alain"}],"intvolume":"       114","isi":1,"file":[{"creator":"kschuh","file_name":"2017_PNAS_Miki.pdf","date_created":"2020-01-03T13:27:29Z","file_id":"7223","file_size":2721544,"content_type":"application/pdf","checksum":"2ab75d554f3df4a34d20fa8040589b7e","date_updated":"2020-07-14T12:47:44Z","access_level":"open_access","relation":"main_file"}],"pmid":1,"oa":1,"publication_identifier":{"issn":["0027-8424"]},"external_id":{"pmid":["28607047"],"isi":["000404108400028"]},"department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"date_created":"2018-12-11T11:47:57Z","language":[{"iso":"eng"}],"corr_author":"1","publication_status":"published","abstract":[{"text":"Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such “simple synapses” indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1–10) and 2.03 at 4 wk (range: 1–4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1–8) and 2.37 at 4 wk (range: 1–5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels. ","lang":"eng"}],"volume":114,"quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"E5246 - E5255","has_accepted_license":"1","date_published":"2017-06-27T00:00:00Z","year":"2017","oa_version":"Published Version","date_updated":"2025-09-10T14:00:03Z","type":"journal_article","publisher":"National Academy of Sciences","day":"27","issue":"26","status":"public","publist_id":"7013","doi":"10.1073/pnas.1704470114","ddc":["570"],"article_processing_charge":"Yes (in subscription journal)","citation":{"ista":"Miki T, Kaufmann W, Malagon G, Gomez L, Tabuchi K, Watanabe M, Shigemoto R, Marty A. 2017. Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. PNAS. 114(26), E5246–E5255.","chicago":"Miki, Takafumi, Walter Kaufmann, Gerardo Malagon, Laura Gomez, Katsuhiko Tabuchi, Masahiko Watanabe, Ryuichi Shigemoto, and Alain Marty. “Numbers of Presynaptic Ca2+ Channel Clusters Match Those of Functionally Defined Vesicular Docking Sites in Single Central Synapses.” <i>PNAS</i>. National Academy of Sciences, 2017. <a href=\"https://doi.org/10.1073/pnas.1704470114\">https://doi.org/10.1073/pnas.1704470114</a>.","apa":"Miki, T., Kaufmann, W., Malagon, G., Gomez, L., Tabuchi, K., Watanabe, M., … Marty, A. (2017). Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1704470114\">https://doi.org/10.1073/pnas.1704470114</a>","ama":"Miki T, Kaufmann W, Malagon G, et al. Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. <i>PNAS</i>. 2017;114(26):E5246-E5255. doi:<a href=\"https://doi.org/10.1073/pnas.1704470114\">10.1073/pnas.1704470114</a>","ieee":"T. Miki <i>et al.</i>, “Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses,” <i>PNAS</i>, vol. 114, no. 26. National Academy of Sciences, pp. E5246–E5255, 2017.","mla":"Miki, Takafumi, et al. “Numbers of Presynaptic Ca2+ Channel Clusters Match Those of Functionally Defined Vesicular Docking Sites in Single Central Synapses.” <i>PNAS</i>, vol. 114, no. 26, National Academy of Sciences, 2017, pp. E5246–55, doi:<a href=\"https://doi.org/10.1073/pnas.1704470114\">10.1073/pnas.1704470114</a>.","short":"T. Miki, W. Kaufmann, G. Malagon, L. Gomez, K. Tabuchi, M. Watanabe, R. Shigemoto, A. Marty, PNAS 114 (2017) E5246–E5255."},"_id":"693","title":"Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses","month":"06"},{"status":"public","day":"21","type":"journal_article","publisher":"Cell Press","issue":"1","oa_version":"None","year":"2017","date_updated":"2025-07-10T11:54:27Z","date_published":"2017-09-21T00:00:00Z","project":[{"_id":"25AD6156-B435-11E9-9278-68D0E5697425","grant_number":"LS13-029","name":"Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments"},{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425"}],"page":"188 - 200","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Load adaptation of lamellipodial actin networks","month":"09","_id":"727","citation":{"ieee":"J. Mueller <i>et al.</i>, “Load adaptation of lamellipodial actin networks,” <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.","mla":"Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>, vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">10.1016/j.cell.2017.07.051</a>.","short":"J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K. Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017) 188–200.","ista":"Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K, Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of lamellipodial actin networks. Cell. 171(1), 188–200.","chicago":"Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber, Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">https://doi.org/10.1016/j.cell.2017.07.051</a>.","apa":"Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C., … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">https://doi.org/10.1016/j.cell.2017.07.051</a>","ama":"Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin networks. <i>Cell</i>. 2017;171(1):188-200. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.051\">10.1016/j.cell.2017.07.051</a>"},"article_processing_charge":"No","doi":"10.1016/j.cell.2017.07.051","publist_id":"6951","publication_identifier":{"issn":["0092-8674"]},"ec_funded":1,"isi":1,"intvolume":"       171","scopus_import":"1","publication":"Cell","author":[{"full_name":"Mueller, Jan","last_name":"Mueller","first_name":"Jan"},{"full_name":"Szep, Gregory","last_name":"Szep","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory"},{"id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","full_name":"Nemethova, Maria","last_name":"Nemethova"},{"full_name":"De Vries, Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid"},{"first_name":"Arnon","last_name":"Lieber","full_name":"Lieber, Arnon"},{"last_name":"Winkler","full_name":"Winkler, Christoph","first_name":"Christoph"},{"first_name":"Karsten","last_name":"Kruse","full_name":"Kruse, Karsten"},{"first_name":"John","full_name":"Small, John","last_name":"Small"},{"first_name":"Christian","last_name":"Schmeiser","full_name":"Schmeiser, Christian"},{"first_name":"Kinneret","full_name":"Keren, Kinneret","last_name":"Keren"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"last_name":"Sixt","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179"}],"quality_controlled":"1","volume":171,"acknowledged_ssus":[{"_id":"ScienComp"}],"abstract":[{"text":"Actin filaments polymerizing against membranes power endocytosis, vesicular traffic, and cell motility. In vitro reconstitution studies suggest that the structure and the dynamics of actin networks respond to mechanical forces. We demonstrate that lamellipodial actin of migrating cells responds to mechanical load when membrane tension is modulated. In a steady state, migrating cell filaments assume the canonical dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension triggers a dense network with a broadened range of angles, whereas decreased tension causes a shift to a sparse configuration dominated by filaments growing perpendicularly to the plasma membrane. We show that these responses emerge from the geometry of branched actin: when load per filament decreases, elongation speed increases and perpendicular filaments gradually outcompete others because they polymerize the shortest distance to the membrane, where they are protected from capping. This network-intrinsic geometrical adaptation mechanism tunes protrusive force in response to mechanical load.","lang":"eng"}],"publication_status":"published","language":[{"iso":"eng"}],"corr_author":"1","department":[{"_id":"MiSi"},{"_id":"Bio"}],"external_id":{"isi":["000411331800020"]},"date_created":"2018-12-11T11:48:10Z"},{"publist_id":"6412","doi":"10.1021/acs.nanolett.7b00097","pubrep_id":"826","ddc":["621"],"citation":{"ama":"Nanda G, Aguilera Servin JL, Rakyta P, et al. Current-phase relation of ballistic graphene Josephson junctions. <i>Nano Letters</i>. 2017;17(6):3396-3401. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">10.1021/acs.nanolett.7b00097</a>","apa":"Nanda, G., Aguilera Servin, J. L., Rakyta, P., Kormányos, A., Kleiner, R., Koelle, D., … Goswami, S. (2017). Current-phase relation of ballistic graphene Josephson junctions. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">https://doi.org/10.1021/acs.nanolett.7b00097</a>","chicago":"Nanda, Gaurav, Juan L Aguilera Servin, Péter Rakyta, Andor Kormányos, Reinhold Kleiner, Dieter Koelle, Kazuo Watanabe, Takashi Taniguchi, Lieven Vandersypen, and Srijit Goswami. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” <i>Nano Letters</i>. American Chemical Society, 2017. <a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">https://doi.org/10.1021/acs.nanolett.7b00097</a>.","ista":"Nanda G, Aguilera Servin JL, Rakyta P, Kormányos A, Kleiner R, Koelle D, Watanabe K, Taniguchi T, Vandersypen L, Goswami S. 2017. Current-phase relation of ballistic graphene Josephson junctions. Nano Letters. 17(6), 3396–3401.","mla":"Nanda, Gaurav, et al. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” <i>Nano Letters</i>, vol. 17, no. 6, American Chemical Society, 2017, pp. 3396–401, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">10.1021/acs.nanolett.7b00097</a>.","short":"G. Nanda, J.L. Aguilera Servin, P. Rakyta, A. Kormányos, R. Kleiner, D. Koelle, K. Watanabe, T. Taniguchi, L. Vandersypen, S. Goswami, Nano Letters 17 (2017) 3396–3401.","ieee":"G. Nanda <i>et al.</i>, “Current-phase relation of ballistic graphene Josephson junctions,” <i>Nano Letters</i>, vol. 17, no. 6. American Chemical Society, pp. 3396–3401, 2017."},"article_processing_charge":"No","title":"Current-phase relation of ballistic graphene Josephson junctions","month":"05","_id":"988","page":"3396 - 3401","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2017","oa_version":"Published Version","date_updated":"2025-07-10T12:02:04Z","date_published":"2017-05-05T00:00:00Z","has_accepted_license":"1","publisher":"American Chemical Society","day":"05","type":"journal_article","issue":"6","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"status":"public","language":[{"iso":"eng"}],"external_id":{"isi":["000403631600011"]},"department":[{"_id":"NanoFab"}],"date_created":"2018-12-11T11:49:33Z","publication_status":"published","abstract":[{"text":"The current-phase relation (CPR) of a Josephson junction (JJ) determines how the supercurrent evolves with the superconducting phase difference across the junction. Knowledge of the CPR is essential in order to understand the response of a JJ to various external parameters. Despite the rising interest in ultraclean encapsulated graphene JJs, the CPR of such junctions remains unknown. Here, we use a fully gate-tunable graphene superconducting quantum intereference device (SQUID) to determine the CPR of ballistic graphene JJs. Each of the two JJs in the SQUID is made with graphene encapsulated in hexagonal boron nitride. By independently controlling the critical current of the JJs, we can operate the SQUID either in a symmetric or asymmetric configuration. The highly asymmetric SQUID allows us to phase-bias one of the JJs and thereby directly obtain its CPR. The CPR is found to be skewed, deviating significantly from a sinusoidal form. The skewness can be tuned with the gate voltage and oscillates in antiphase with Fabry-Pérot resistance oscillations of the ballistic graphene cavity. We compare our experiments with tight-binding calculations that include realistic graphene-superconductor interfaces and find a good qualitative agreement.","lang":"eng"}],"quality_controlled":"1","volume":17,"isi":1,"intvolume":"        17","publication":"Nano Letters","file_date_updated":"2020-07-14T12:48:18Z","author":[{"first_name":"Gaurav","full_name":"Nanda, Gaurav","last_name":"Nanda"},{"first_name":"Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87","last_name":"Aguilera Servin","full_name":"Aguilera Servin, Juan L","orcid":"0000-0002-2862-8372"},{"full_name":"Rakyta, Péter","last_name":"Rakyta","first_name":"Péter"},{"first_name":"Andor","last_name":"Kormányos","full_name":"Kormányos, Andor"},{"full_name":"Kleiner, Reinhold","last_name":"Kleiner","first_name":"Reinhold"},{"first_name":"Dieter","last_name":"Koelle","full_name":"Koelle, Dieter"},{"full_name":"Watanabe, Kazuo","last_name":"Watanabe","first_name":"Kazuo"},{"first_name":"Takashi","last_name":"Taniguchi","full_name":"Taniguchi, Takashi"},{"first_name":"Lieven","last_name":"Vandersypen","full_name":"Vandersypen, Lieven"},{"first_name":"Srijit","full_name":"Goswami, Srijit","last_name":"Goswami"}],"scopus_import":"1","publication_identifier":{"issn":["1530-6984"]},"oa":1,"file":[{"access_level":"open_access","relation":"main_file","file_size":508638,"file_name":"IST-2017-826-v1+1_2017_Aguilera-Servin_Current.pdf","creator":"system","date_created":"2018-12-12T10:13:50Z","file_id":"5037","date_updated":"2020-07-14T12:48:18Z","checksum":"22021daa90cf13b01becd776838acb7b","content_type":"application/pdf"}]},{"ddc":["570"],"article_type":"original","publist_id":"7047","doi":"10.1242/dev.144964","_id":"676","month":"05","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","article_processing_charge":"No","related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"},{"relation":"dissertation_contains","status":"public","id":"50"}]},"citation":{"apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., &#38; Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>","ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. 2017;144(10):1798-1806. doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806.","chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>. Company of Biologists, 2017. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>.","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>.","short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806.","ieee":"G. Krens <i>et al.</i>, “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” <i>Development</i>, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017."},"date_published":"2017-05-15T00:00:00Z","has_accepted_license":"1","date_updated":"2026-04-28T22:31:01Z","year":"2017","oa_version":"Published Version","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"1798 - 1806","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"issue":"10","type":"journal_article","publisher":"Company of Biologists","day":"15","publication_status":"published","date_created":"2018-12-11T11:47:52Z","external_id":{"isi":["000402275900007"],"pmid":["28512197"]},"department":[{"_id":"Bio"},{"_id":"CaHe"}],"language":[{"iso":"eng"}],"corr_author":"1","volume":144,"quality_controlled":"1","abstract":[{"text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.","lang":"eng"}],"scopus_import":"1","publication":"Development","file_date_updated":"2020-07-14T12:47:39Z","author":[{"full_name":"Krens, Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","orcid":"0000-0003-4761-5996"},{"full_name":"Veldhuis, Jim","last_name":"Veldhuis","first_name":"Jim"},{"orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","full_name":"Barone, Vanessa","last_name":"Barone"},{"orcid":"0000-0001-5199-9940","full_name":"Capek, Daniel","last_name":"Capek","id":"31C42484-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel"},{"last_name":"Maître","full_name":"Maître, Jean-Léon","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474"},{"last_name":"Brodland","full_name":"Brodland, Wayne","first_name":"Wayne"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"intvolume":"       144","isi":1,"pmid":1,"file":[{"relation":"main_file","access_level":"open_access","file_size":8194516,"date_created":"2019-09-24T06:56:22Z","file_id":"6905","creator":"dernst","file_name":"2017_Development_Krens.pdf","date_updated":"2020-07-14T12:47:39Z","content_type":"application/pdf","checksum":"bc25125fb664706cdf180e061429f91d"}],"oa":1,"publication_identifier":{"issn":["0950-1991"]}},{"language":[{"iso":"eng"}],"corr_author":"1","external_id":{"isi":["000397917000009"],"pmid":["28346437"]},"department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"date_created":"2018-12-11T11:47:46Z","publication_status":"published","acknowledged_ssus":[{"_id":"SSU"}],"abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"quality_controlled":"1","volume":19,"ec_funded":1,"isi":1,"intvolume":"        19","scopus_import":"1","publication":"Nature Cell Biology","author":[{"orcid":"0000-0002-5920-9090","full_name":"Smutny, Michael","last_name":"Smutny","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ákos","full_name":"Ákos, Zsuzsa","first_name":"Zsuzsa"},{"full_name":"Grigolon, Silvia","last_name":"Grigolon","first_name":"Silvia"},{"last_name":"Shamipour","full_name":"Shamipour, Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan"},{"first_name":"Verena","full_name":"Ruprecht, Verena","last_name":"Ruprecht"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Capek","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940"},{"full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"first_name":"Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","full_name":"Papusheva, Ekaterina","last_name":"Papusheva"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"orcid":"0000-0003-2057-2754","first_name":"Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","full_name":"Hof, Björn"},{"first_name":"Tamás","last_name":"Vicsek","full_name":"Vicsek, Tamás"},{"last_name":"Salbreux","full_name":"Salbreux, Guillaume","first_name":"Guillaume"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"main_file_link":[{"url":"https://europepmc.org/articles/pmc5635970","open_access":"1"}],"publication_identifier":{"issn":["1465-7392"]},"oa":1,"pmid":1,"publist_id":"7074","doi":"10.1038/ncb3492","citation":{"ieee":"M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317.","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>.","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>.","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>"},"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"8350"},{"relation":"dissertation_contains","status":"public","id":"50"}]},"article_processing_charge":"No","title":"Friction forces position the neural anlage","month":"03","_id":"661","page":"306 - 317","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa_version":"Submitted Version","year":"2017","date_updated":"2026-04-28T22:31:01Z","date_published":"2017-03-27T00:00:00Z","project":[{"grant_number":"306589","_id":"25152F3A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Decoding the complexity of turbulence at its origin"},{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I930-B20","call_identifier":"FWF","name":"Control of Epithelial Cell Layer Spreading in Zebrafish"}],"day":"27","type":"journal_article","publisher":"Nature Publishing Group","status":"public"},{"publist_id":"6204","doi":"10.1038/srep36440","ddc":["579"],"pubrep_id":"744","article_processing_charge":"No","citation":{"ieee":"J. Schwarz <i>et al.</i>, “A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016.","short":"J. Schwarz, V. Bierbaum, J. Merrin, T. Frank, R. Hauschild, M.T. Bollenbach, S. Tay, M.K. Sixt, M. Mehling, Scientific Reports 6 (2016).","mla":"Schwarz, Jan, et al. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” <i>Scientific Reports</i>, vol. 6, 36440, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep36440\">10.1038/srep36440</a>.","chicago":"Schwarz, Jan, Veronika Bierbaum, Jack Merrin, Tino Frank, Robert Hauschild, Mark Tobias Bollenbach, Savaş Tay, Michael K Sixt, and Matthias Mehling. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep36440\">https://doi.org/10.1038/srep36440</a>.","ista":"Schwarz J, Bierbaum V, Merrin J, Frank T, Hauschild R, Bollenbach MT, Tay S, Sixt MK, Mehling M. 2016. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. Scientific Reports. 6, 36440.","ama":"Schwarz J, Bierbaum V, Merrin J, et al. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep36440\">10.1038/srep36440</a>","apa":"Schwarz, J., Bierbaum, V., Merrin, J., Frank, T., Hauschild, R., Bollenbach, M. T., … Mehling, M. (2016). A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep36440\">https://doi.org/10.1038/srep36440</a>"},"_id":"1154","month":"11","title":"A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425"}],"has_accepted_license":"1","date_published":"2016-11-07T00:00:00Z","date_updated":"2025-09-22T09:56:13Z","oa_version":"Published Version","year":"2016","publisher":"Nature Publishing Group","day":"07","type":"journal_article","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_created":"2018-12-11T11:50:27Z","external_id":{"isi":["000387118300001"]},"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"ToBo"}],"language":[{"iso":"eng"}],"publication_status":"published","article_number":"36440","abstract":[{"lang":"eng","text":"Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. \r\n"}],"volume":6,"quality_controlled":"1","author":[{"full_name":"Schwarz, Jan","last_name":"Schwarz","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","first_name":"Jan"},{"last_name":"Bierbaum","full_name":"Bierbaum, Veronika","id":"3FD04378-F248-11E8-B48F-1D18A9856A87","first_name":"Veronika"},{"first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"full_name":"Frank, Tino","last_name":"Frank","first_name":"Tino"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"orcid":"0000-0003-4398-476X","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","first_name":"Mark Tobias"},{"first_name":"Savaş","last_name":"Tay","full_name":"Tay, Savaş"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"3C23B994-F248-11E8-B48F-1D18A9856A87","first_name":"Matthias","full_name":"Mehling, Matthias","last_name":"Mehling","orcid":"0000-0001-8599-1226"}],"file_date_updated":"2018-12-12T10:09:32Z","publication":"Scientific Reports","scopus_import":"1","intvolume":"         6","isi":1,"ec_funded":1,"acknowledgement":"This work was supported by the Swiss National Science Foundation (Ambizione fellowship; PZ00P3-154733 to M.M.), the Swiss Multiple Sclerosis Society (research support to M.M.), a fellowship from the Boehringer Ingelheim Fonds (BIF) to J.S., the European Research Council (grant ERC GA 281556) and a START award from the Austrian Science Foundation (FWF) to M.S. #BioimagingFacility","file":[{"content_type":"application/pdf","date_updated":"2018-12-12T10:09:32Z","file_id":"4756","date_created":"2018-12-12T10:09:32Z","creator":"system","file_name":"IST-2017-744-v1+1_srep36440.pdf","file_size":2353456,"relation":"main_file","access_level":"open_access"}],"oa":1},{"abstract":[{"text":"The hippocampal CA3 region plays a key role in learning and memory. Recurrent CA3–CA3\r\nsynapses are thought to be the subcellular substrate of pattern completion. However, the\r\nsynaptic mechanisms of this network computation remain enigmatic. To investigate these mechanisms, we combined functional connectivity analysis with network modeling.\r\nSimultaneous recording fromup to eight CA3 pyramidal neurons revealed that connectivity was sparse, spatially uniform, and highly enriched in disynaptic motifs (reciprocal, convergence,divergence, and chain motifs). Unitary connections were composed of one or two synaptic contacts, suggesting efficient use of postsynaptic space. Real-size modeling indicated that CA3 networks with sparse connectivity, disynaptic motifs, and single-contact connections robustly generated pattern completion.Thus, macro- and microconnectivity contribute to efficient\r\nmemory storage and retrieval in hippocampal networks.","lang":"eng"}],"acknowledged_ssus":[{"_id":"ScienComp"}],"volume":353,"quality_controlled":"1","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"external_id":{"isi":["000382626800045"]},"date_created":"2018-12-11T11:51:31Z","corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published","file":[{"file_name":"IST-2017-823-v1+1_aaf1836_CombinedPDF_v2-1.pdf","creator":"system","file_id":"4945","date_created":"2018-12-12T10:12:27Z","file_size":19408143,"content_type":"application/pdf","checksum":"89caefa4e181424cbf0aecc835fcc5ec","date_updated":"2020-07-14T12:44:46Z","access_level":"open_access","relation":"main_file"}],"oa":1,"author":[{"id":"30CC5506-F248-11E8-B48F-1D18A9856A87","first_name":"José","last_name":"Guzmán","full_name":"Guzmán, José","orcid":"0000-0003-2209-5242"},{"orcid":"0000-0002-5621-8100","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","full_name":"Schlögl, Alois","last_name":"Schlögl"},{"last_name":"Frotscher","full_name":"Frotscher, Michael","first_name":"Michael"},{"full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804"}],"scopus_import":"1","file_date_updated":"2020-07-14T12:44:46Z","publication":"Science","ec_funded":1,"intvolume":"       353","isi":1,"article_processing_charge":"No","citation":{"ista":"Guzmán J, Schlögl A, Frotscher M, Jonas PM. 2016. Synaptic mechanisms of pattern completion in the hippocampal CA3 network. Science. 353(6304), 1117–1123.","chicago":"Guzmán, José, Alois Schlögl, Michael Frotscher, and Peter M Jonas. “Synaptic Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” <i>Science</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/science.aaf1836\">https://doi.org/10.1126/science.aaf1836</a>.","apa":"Guzmán, J., Schlögl, A., Frotscher, M., &#38; Jonas, P. M. (2016). Synaptic mechanisms of pattern completion in the hippocampal CA3 network. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aaf1836\">https://doi.org/10.1126/science.aaf1836</a>","ama":"Guzmán J, Schlögl A, Frotscher M, Jonas PM. Synaptic mechanisms of pattern completion in the hippocampal CA3 network. <i>Science</i>. 2016;353(6304):1117-1123. doi:<a href=\"https://doi.org/10.1126/science.aaf1836\">10.1126/science.aaf1836</a>","ieee":"J. Guzmán, A. Schlögl, M. Frotscher, and P. M. Jonas, “Synaptic mechanisms of pattern completion in the hippocampal CA3 network,” <i>Science</i>, vol. 353, no. 6304. American Association for the Advancement of Science, pp. 1117–1123, 2016.","mla":"Guzmán, José, et al. “Synaptic Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” <i>Science</i>, vol. 353, no. 6304, American Association for the Advancement of Science, 2016, pp. 1117–23, doi:<a href=\"https://doi.org/10.1126/science.aaf1836\">10.1126/science.aaf1836</a>.","short":"J. Guzmán, A. Schlögl, M. Frotscher, P.M. 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AHPC: Austrian HPC Meeting, 37.","chicago":"Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST Austria: Modelling the Human Hippocampus.” In <i>AHPC16 - Austrian HPC Meeting 2016</i>, 37. 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