[{"OA_place":"repository","OA_type":"green","language":[{"iso":"eng"}],"publication":"bioRxiv","acknowledgement":"We would like to thank the members of the Sweeney Lab for discussion and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions of the NIH author are considered Works of the United States\r\nGovernment. The findings and conclusions presented in this paper are those of\r\nthe authors and do not necessarily reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. ","project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"grant_number":"27229","name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis","_id":"907b765e-16d5-11f0-9cad-fef108a945b1"}],"citation":{"chicago":"Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.09.680955\">https://doi.org/10.1101/2025.10.09.680955</a>.","apa":"Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka, E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity across vertebrate evolution. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.09.680955\">https://doi.org/10.1101/2025.10.09.680955</a>","mla":"Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>.","ama":"Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>","ieee":"Y. Ignatyev <i>et al.</i>, “Innovations in spinal cord cell type heterogeneity across vertebrate evolution,” <i>bioRxiv</i>. .","ista":"Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>.","short":"Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka, L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.)."},"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_updated":"2026-05-27T07:25:41Z","status":"public","date_created":"2026-05-27T06:54:04Z","author":[{"full_name":"Ignatyev, Yuri","last_name":"Ignatyev","first_name":"Yuri"},{"full_name":"Papadopoulos, Stavros","id":"40606b92-f128-11eb-9611-bf66a98cfa5c","first_name":"Stavros","last_name":"Papadopoulos"},{"last_name":"Soretić","first_name":"Mateja","full_name":"Soretić, Mateja"},{"first_name":"Jake","last_name":"Yeung","id":"123012b2-db30-11eb-b4d8-a35840c0551b","full_name":"Yeung, Jake","orcid":"0000-0003-1732-1559"},{"full_name":"Lin, Tzi-Yang","last_name":"Lin","first_name":"Tzi-Yang"},{"full_name":"Tanaka, Elly M","first_name":"Elly M","last_name":"Tanaka"},{"full_name":"Peshkin, Leonid","last_name":"Peshkin","first_name":"Leonid"},{"full_name":"Levine, Ariel J","first_name":"Ariel J","last_name":"Levine"},{"last_name":"Gabitto","first_name":"Mariano I","full_name":"Gabitto, Mariano I"},{"last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}],"_id":"21920","title":"Innovations in spinal cord cell type heterogeneity across vertebrate evolution","abstract":[{"text":"Vertebrates display remarkable diversity of sensorimotor behaviors, each adapted to distinct ecological and survival demands. This diversity raises fundamental questions about the evolutionary origin of motor control: do conserved spinal circuits underlie these behaviors, and how have they diverged across species. Recent studies detail spinal cell-type architecture in mammals but comparable, high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we compare spinal cord cell types between fish, frogs, mice and humans, spanning ∼450 million years of evolution. Across species, we define highly conserved programs of cell type specification that segregate spinal neurons into nearly identical cardinal classes during development. This contrasts with adult stages, when spinal cell-type composition selectively diverges for excitatory neuron subpopulations. Using spatial transcriptomics, we localize this species divergence to the superficial, dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific cell types. The most dorsal spinal cord thus emerges as a recently evolved hub for sensory integration in mammals, a neospinal cord analogous to the neocortex.</jats:p>","lang":"eng"}],"oa_version":"Preprint","type":"preprint","doi":"10.1101/2025.10.09.680955","corr_author":"1","oa":1,"day":"11","department":[{"_id":"LoSw"},{"_id":"ScienComp"}],"month":"10","article_processing_charge":"No","date_published":"2025-10-11T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1101/2025.10.09.680955","open_access":"1"}],"fulldoi":"https://doi.org/10.1101/2025.10.09.680955","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"year":"2025","publication_status":"submitted"},{"doi":"10.1038/s41593-025-01874-w","volume":28,"isi":1,"article_number":"7278","publisher":"Springer Nature","department":[{"_id":"MaJö"},{"_id":"PreCl"}],"month":"03","external_id":{"isi":["001416866800001"],"pmid":["39930095"]},"corr_author":"1","oa":1,"day":"01","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"Bio"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41593-025-01874-w"}],"fulldoi":"https://doi.org/10.1038/s41593-025-01874-w","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","date_published":"2025-03-01T00:00:00Z","intvolume":"        28","publication_status":"published","quality_controlled":"1","year":"2025","pmid":1,"ddc":["570"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"hybrid","ec_funded":1,"publication_identifier":{"issn":["1097-6256"],"eissn":["1546-1726"]},"acknowledgement":"We thank Y. Ben-Simon for generously making viral vectors for retrograde tracing available, as well as J. Watson and F. Marr for reagents. We also thank R. Shigemoto, W. Młynarski and members of the Neuroethology group for their comments on the manuscript and L. Burnett for her schematic drawings. This research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Preclinical Facility, the Lab Support Facility and the Imaging and Optics Facility, in particular F. Lange, M. Schunn and T. Asenov. This work was supported by European Research Council Starting Grant no. 756502 (M.J.) and European Research Council Consolidator Grant no. 101086580 (M.J.); and EMBO ALTF grant no. 1098-2017 (A.S.) and Human Frontiers Science Program grant no. LT000256/2018-L (A.S.). Open access funding provided by Institute of Science and Technology (IST Austria).","scopus_import":"1","publication":"Nature Neuroscience","date_updated":"2026-06-18T18:12:08Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Vega Zuniga, Tomas A, Anton L Sumser, Olga Symonova, Peter Koppensteiner, Florian Schmidt, and Maximilian A Jösch. “A Thalamic Hub-and-Spoke Network Enables Visual Perception during Action by Coordinating Visuomotor Dynamics.” <i>Nature Neuroscience</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41593-025-01874-w\">https://doi.org/10.1038/s41593-025-01874-w</a>.","apa":"Vega Zuniga, T. A., Sumser, A. L., Symonova, O., Koppensteiner, P., Schmidt, F., &#38; Jösch, M. A. (2025). A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. <i>Nature Neuroscience</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41593-025-01874-w\">https://doi.org/10.1038/s41593-025-01874-w</a>","mla":"Vega Zuniga, Tomas A., et al. “A Thalamic Hub-and-Spoke Network Enables Visual Perception during Action by Coordinating Visuomotor Dynamics.” <i>Nature Neuroscience</i>, vol. 28, 7278, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41593-025-01874-w\">10.1038/s41593-025-01874-w</a>.","ama":"Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA. A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. <i>Nature Neuroscience</i>. 2025;28. doi:<a href=\"https://doi.org/10.1038/s41593-025-01874-w\">10.1038/s41593-025-01874-w</a>","ieee":"T. A. Vega Zuniga, A. L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt, and M. A. Jösch, “A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics,” <i>Nature Neuroscience</i>, vol. 28. Springer Nature, 2025.","ista":"Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA. 2025. A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. Nature Neuroscience. 28, 7278.","short":"T.A. Vega Zuniga, A.L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt, M.A. Jösch, Nature Neuroscience 28 (2025)."},"project":[{"_id":"2634E9D2-B435-11E9-9278-68D0E5697425","name":"Circuits of Visual Attention","call_identifier":"H2020","grant_number":"756502"},{"grant_number":"101086580","name":"Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses","_id":"bdaf81a8-d553-11ed-ba76-c95961984540"},{"grant_number":"ALTF 1098-2017","name":"Connecting sensory with motor processing in the superior colliculus","_id":"264FEA02-B435-11E9-9278-68D0E5697425"},{"grant_number":"LT000256","name":"Neuronal networks of salience and spatial detection in the murine superior colliculus","_id":"266D407A-B435-11E9-9278-68D0E5697425"}],"license":"https://creativecommons.org/licenses/by/4.0/","related_material":{"record":[{"status":"public","relation":"research_data","id":"18579"}],"link":[{"url":"https://ista.ac.at/en/news/high-tech-video-optimization-in-our-brain/","relation":"press_release","description":"News on ISTA Website"}]},"abstract":[{"text":"For accurate perception and motor control, an animal must distinguish between sensory experiences elicited by external stimuli and those elicited by its own actions. The diversity of behaviors and their complex influences on the senses make this distinction challenging. Here, we uncover an action–cue hub that coordinates motor commands with visual processing in the brain’s first visual relay. We show that the ventral lateral geniculate nucleus (vLGN) acts as a corollary discharge center, integrating visual translational optic flow signals with motor copies from saccades, locomotion and pupil dynamics. The vLGN relays these signals to correct action-specific visual distortions and to refine perception, as shown for the superior colliculus and in a depth-estimation task. Simultaneously, brain-wide vLGN projections drive corrective actions necessary for accurate visuomotor control. Our results reveal an extended corollary discharge architecture that refines early visual transformations and coordinates actions via a distributed hub-and-spoke network to enable visual perception during action.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","article_type":"original","author":[{"id":"2E7C4E78-F248-11E8-B48F-1D18A9856A87","last_name":"Vega Zuniga","first_name":"Tomas A","full_name":"Vega Zuniga, Tomas A"},{"orcid":"0000-0002-4792-1881","full_name":"Sumser, Anton L","last_name":"Sumser","first_name":"Anton L","id":"3320A096-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Olga","last_name":"Symonova","id":"3C0C7BC6-F248-11E8-B48F-1D18A9856A87","full_name":"Symonova, Olga","orcid":"0000-0003-2012-9947"},{"orcid":"0000-0002-3509-1948","full_name":"Koppensteiner, Peter","last_name":"Koppensteiner","first_name":"Peter","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87"},{"id":"A2EF226A-AF19-11E9-924C-0525E6697425","first_name":"Florian","last_name":"Schmidt","full_name":"Schmidt, Florian"},{"orcid":"0000-0002-3937-1330","full_name":"Jösch, Maximilian A","first_name":"Maximilian A","last_name":"Jösch","id":"2BD278E6-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2025-02-23T23:01:58Z","status":"public","title":"A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics","_id":"19076"},{"abstract":[{"text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the non-canonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g. control of costly-to-induce multi-drug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multi-drug resistance is crucial for effective public health measures.","lang":"eng"}],"year":"2025","type":"research_data","oa_version":"Published Version","date_created":"2025-03-04T13:27:21Z","status":"public","author":[{"id":"330F0278-F248-11E8-B48F-1D18A9856A87","first_name":"Kirti","last_name":"Jain","full_name":"Jain, Kirti","orcid":"0000-0002-3809-0449"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild"},{"id":"C4558D3C-6102-11E9-A62E-F418E6697425","first_name":"Olga","last_name":"Bochkareva","full_name":"Bochkareva, Olga","orcid":"0000-0003-1006-6639"},{"last_name":"Römhild","first_name":"Roderich","id":"68E56E44-62B0-11EA-B963-444F3DDC885E","full_name":"Römhild, Roderich","orcid":"0000-0001-9480-5261"},{"first_name":"Gašper","last_name":"Tkačik","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkačik, Gašper","orcid":"0000-0002-6699-1455"},{"full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","first_name":"Calin C","last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87"}],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"_id":"19294","title":"Data for \"Pulsatile basal gene expression as a fitness determinant in bacteria\"","fulldoi":"https://doi.org/10.15479/AT:ISTA:19294","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-05-20T08:33:07Z","article_processing_charge":"No","citation":{"apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’” Institute of Science and Technology Austria, 2025.","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>.","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” 2025. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>","mla":"Jain, Kirti, et al. <i>Data for “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.”</i> Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>."},"date_published":"2025-03-04T00:00:00Z","related_material":{"record":[{"relation":"used_in_publication","id":"19626","status":"public"}]},"file_date_updated":"2025-03-05T07:39:38Z","has_accepted_license":"1","month":"03","department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"publisher":"Institute of Science and Technology Austria","oa":1,"corr_author":"1","day":"04","file":[{"file_size":269054,"file_name":"Data1.xlsx","date_updated":"2025-03-04T13:08:52Z","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_id":"19295","creator":"dernst","relation":"main_file","access_level":"open_access","date_created":"2025-03-04T13:08:52Z","checksum":"11a5bab307a4e1e1598a1577d8a2fbb5","success":1},{"access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"3b057894322639f0c1e11fb2e84173e6","date_created":"2025-03-04T13:08:52Z","file_name":"Data2.xlsx","date_updated":"2025-03-04T13:08:52Z","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_size":87143,"file_id":"19296"},{"date_updated":"2025-03-04T13:08:52Z","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_name":"Data3.xlsx","file_size":129101,"file_id":"19297","access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"a551e1b79a138bb97ab96979aa475b3c","date_created":"2025-03-04T13:08:52Z"},{"creator":"dernst","relation":"main_file","access_level":"open_access","date_created":"2025-03-04T13:08:52Z","checksum":"d6909c9bf111f859058082b1a2f970c4","success":1,"file_size":86243,"content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","date_updated":"2025-03-04T13:08:52Z","file_name":"Data4.xlsx","file_id":"19298"},{"content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","date_updated":"2025-03-04T13:08:52Z","file_name":"Data5.xlsx","file_size":26049,"file_id":"19299","access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"e5725a3a118a3f06846104906c8792c7","date_created":"2025-03-04T13:08:52Z"},{"file_size":7327253,"file_name":"RawData_2_3.xlsx","date_updated":"2025-03-04T13:08:52Z","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_id":"19300","relation":"main_file","creator":"dernst","access_level":"open_access","checksum":"16763c127049f14bd587dc885677dce1","date_created":"2025-03-04T13:08:52Z","success":1},{"date_updated":"2025-03-05T07:39:38Z","content_type":"text/plain","file_name":"Readme.txt","file_size":606,"file_id":"19301","access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"date_created":"2025-03-05T07:39:38Z","checksum":"2f3e1a368b4e3abc46bf37e02724f0f4"}],"doi":"10.15479/AT:ISTA:19294","OA_place":"repository","OA_type":"gold"},{"year":"2025","quality_controlled":"1","pmid":1,"publication_status":"published","intvolume":"       122","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"fulldoi":"https://doi.org/10.1073/pnas.2413709122","APC_amount":"5949 EUR","acknowledged_ssus":[{"_id":"Bio"}],"file_date_updated":"2025-06-24T07:27:43Z","date_published":"2025-04-15T00:00:00Z","has_accepted_license":"1","article_processing_charge":"Yes (in subscription journal)","external_id":{"isi":["001471235200001"],"pmid":["40193613"]},"month":"04","department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"publisher":"National Academy of Sciences","day":"15","oa":1,"corr_author":"1","volume":122,"file":[{"creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"115a687f40009660eb4b38b4f6559d41","date_created":"2025-06-24T07:27:43Z","success":1,"file_size":2949523,"content_type":"application/pdf","date_updated":"2025-06-24T07:27:43Z","file_name":"2025_PNAS_Jain.pdf","file_id":"19888"}],"doi":"10.1073/pnas.2413709122","article_number":"e2413709122","isi":1,"article_type":"original","type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the noncanonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures.","lang":"eng"}],"_id":"19626","title":"Pulsatile basal gene expression as a fitness determinant in bacteria","author":[{"full_name":"Jain, Kirti","orcid":"0000-0002-3809-0449","id":"330F0278-F248-11E8-B48F-1D18A9856A87","first_name":"Kirti","last_name":"Jain"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"full_name":"Bochkareva, Olga","orcid":"0000-0003-1006-6639","id":"C4558D3C-6102-11E9-A62E-F418E6697425","last_name":"Bochkareva","first_name":"Olga"},{"id":"68E56E44-62B0-11EA-B963-444F3DDC885E","first_name":"Roderich","last_name":"Römhild","full_name":"Römhild, Roderich","orcid":"0000-0001-9480-5261"},{"orcid":"0000-0002-6699-1455","full_name":"Tkačik, Gašper","first_name":"Gašper","last_name":"Tkačik","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C","last_name":"Guet"}],"status":"public","date_created":"2025-04-27T22:02:13Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-05-20T08:33:08Z","related_material":{"record":[{"status":"public","relation":"research_data","id":"19294"}],"link":[{"url":"https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/","relation":"press_release","description":"News on ISTA website"}]},"project":[{"grant_number":"CZI01","name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"grant_number":"E219","name":"Non-canonical antibiotic interactions","_id":"bd6f94d1-d553-11ed-ba76-ae9f07250f74"},{"grant_number":"I05127","name":"Evolutionary analysis of gene regulation","_id":"34e076d6-11ca-11ed-8bc3-aec76c41a181"}],"citation":{"ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Pulsatile basal gene expression as a fitness determinant in bacteria,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15. National Academy of Sciences, 2025.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings of the National Academy of Sciences 122 (2025).","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 122(15), e2413709122.","mla":"Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15, e2413709122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>.","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(15). doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>."},"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"acknowledgement":"K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions on the manuscript, all other members from the Guet laboratory for valuable feedback, R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic Technology India for allowing time off to address the revisions. K.J. acknowledges Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi), O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.","publication":"Proceedings of the National Academy of Sciences","issue":"15","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher"},{"intvolume":"        11","publication_status":"published","quality_controlled":"1","year":"2025","pmid":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"fulldoi":"https://doi.org/10.1126/sciadv.adx4047","article_processing_charge":"Yes","has_accepted_license":"1","file_date_updated":"2025-05-12T07:46:10Z","date_published":"2025-04-25T00:00:00Z","publisher":"AAAS","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"month":"04","external_id":{"pmid":["40279414"],"isi":["001476113400016"]},"oa":1,"day":"25","doi":"10.1126/sciadv.adx4047","file":[{"file_name":"2025_ScienceAdvance_Schmitt.pdf","date_updated":"2025-05-12T07:46:10Z","content_type":"application/pdf","file_size":2707050,"file_id":"19679","access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"e8ba22922fa5b23ccfcce8865f57226c","date_created":"2025-05-12T07:46:10Z"}],"volume":11,"isi":1,"article_number":"eadx4047","abstract":[{"lang":"eng","text":"The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules that grow radially and exert forces on cargos. At the same time, mechanical stresses from the microenvironment and cellular shape changes compress and bend microtubules. Yet, centrosomes are membraneless organelles, raising the question of how centrosomes withstand mechanical forces. Here, we discover that centrosomes can deform and even fracture. We reveal that centrosomes experience deformations during navigational pathfinding within motile cells. Coherence of the centrosome is maintained by Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes cellular function by generating coexisting microtubule organizing centers that compete during path navigation and thereby cause cellular entanglement in the microenvironment. Our findings show that cells actively maintain the integrity of the centrosome to withstand mechanical forces. These results suggest that centrosome stability preservation is fundamental, given that almost all cells in multicellular organisms experience forces."}],"oa_version":"Published Version","type":"journal_article","article_type":"original","author":[{"last_name":"Schmitt","first_name":"Madeleine T.","full_name":"Schmitt, Madeleine T."},{"last_name":"Kroll","first_name":"Janina","full_name":"Kroll, Janina"},{"full_name":"Ruiz-Fernandez, Mauricio J.A.","first_name":"Mauricio J.A.","last_name":"Ruiz-Fernandez"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"last_name":"Ghosh","first_name":"Shaunak","full_name":"Ghosh, Shaunak"},{"last_name":"Kameritsch","first_name":"Petra","full_name":"Kameritsch, Petra"},{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack"},{"last_name":"Schmid","first_name":"Johanna","full_name":"Schmid, Johanna"},{"full_name":"Stefanowski, Kasia","last_name":"Stefanowski","first_name":"Kasia"},{"full_name":"Thomae, Andreas W.","last_name":"Thomae","first_name":"Andreas W."},{"full_name":"Cheng, Jingyuan","last_name":"Cheng","first_name":"Jingyuan"},{"first_name":"Gamze Naz","last_name":"Öztan","full_name":"Öztan, Gamze Naz"},{"full_name":"Konopka, Peter","last_name":"Konopka","first_name":"Peter"},{"full_name":"Ortega, Germán Camargo","first_name":"Germán Camargo","last_name":"Ortega"},{"last_name":"Penz","first_name":"Thomas","full_name":"Penz, Thomas"},{"full_name":"Bach, Luisa","last_name":"Bach","first_name":"Luisa"},{"last_name":"Baumjohann","first_name":"Dirk","full_name":"Baumjohann, Dirk"},{"full_name":"Bock, Christoph","first_name":"Christoph","last_name":"Bock"},{"first_name":"Tobias","last_name":"Straub","full_name":"Straub, Tobias"},{"full_name":"Meissner, Felix","first_name":"Felix","last_name":"Meissner"},{"first_name":"Eva","last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","full_name":"Kiermaier, Eva"},{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","first_name":"Jörg","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369"}],"status":"public","date_created":"2025-05-11T22:02:38Z","title":"Protecting centrosomes from fracturing enables efficient cell navigation","_id":"19663","date_updated":"2025-09-30T12:26:21Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"short":"M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P. Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N. Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub, F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).","ieee":"M. T. Schmitt <i>et al.</i>, “Protecting centrosomes from fracturing enables efficient cell navigation,” <i>Science Advances</i>, vol. 11, no. 17. AAAS, 2025.","ista":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P, Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 11(17), eadx4047.","mla":"Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>, vol. 11, no. 17, eadx4047, AAAS, 2025, doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>.","ama":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. 2025;11(17). doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>","apa":"Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh, S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>","chicago":"Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>. AAAS, 2025. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>."},"DOAJ_listed":"1","project":[{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"publication_identifier":{"eissn":["2375-2548"]},"acknowledgement":"We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids; M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids; M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts, Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support; M. Benjamin Braun for critical reading of the manuscript; and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.); German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (J.R.); Medical & Clinician Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048 (D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag 457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.); Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments (E.K.); and CZI grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (R.H.).","scopus_import":"1","issue":"17","publication":"Science Advances","language":[{"iso":"eng"}],"OA_type":"gold","OA_place":"publisher"},{"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NMR"},{"_id":"LifeSc"}],"fulldoi":"https://doi.org/10.29363/nanoge.matsusspring.2025.173","article_processing_charge":"No","conference":{"location":"Sevilla, Spain","name":"MATSUS: Materials for Sustainable Development Conference","start_date":"2025-03-03","end_date":"2025-03-07"},"date_published":"2025-03-15T00:00:00Z","publication_status":"published","quality_controlled":"1","year":"2025","doi":"10.29363/nanoge.matsusspring.2025.173","article_number":"173","publisher":"Fundació de la comunitat valenciana SCITO","month":"03","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"corr_author":"1","day":"15","date_updated":"2026-02-19T09:25:57Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil, and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” In <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Fundació de la comunitat valenciana SCITO, 2025. <a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">https://doi.org/10.29363/nanoge.matsusspring.2025.173</a>.","apa":"Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., &#38; Ibáñez, M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. <a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">https://doi.org/10.29363/nanoge.matsusspring.2025.173</a>","ama":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In: <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Fundació de la comunitat valenciana SCITO; 2025. doi:<a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">10.29363/nanoge.matsusspring.2025.173</a>","mla":"Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” <i>Proceedings of the MATSUS Spring 2025 Conference</i>, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:<a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">10.29363/nanoge.matsusspring.2025.173</a>.","ieee":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case,” in <i>Proceedings of the MATSUS Spring 2025 Conference</i>, Sevilla, Spain, 2025.","short":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:, Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana SCITO, 2025.","ista":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS: Materials for Sustainable Development Conference, 173."},"project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"abstract":[{"text":"Supercrystals represent three-dimensional orderings of colloidal nanocrystals (NCs), showcasing collective properties in photonics, phononics, and electronics applications.1,2 Recent studies have shown that such assemblies are directly produced during nanocrystal reactions.3–6 However, a fundamental understanding of in situ formed supercrystals that withstand typical NC purification processes remains underexplored, which is important for further use. Herein, we report the reaction precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation of these assemblies through small-angle x-ray scattering (SAXS) measurements, we unveil their formation mechanism. Our findings reveal that the supercrystal formation occurs in the presence of an excess of lead oleates in the crude solution. It should be noted that the formed supercrystals can be stabilized under specific conditions determined by the lead oleate cluster concentration, content of trioctylphosphine telluride (TOP-Te), NC size and the need of an annealing step at mild conditions. Furthermore, this approach allows for the continuous growth of a secondary phase within the supercrystal; for example in the case of PbTe supercrystals, a PbS shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS supercrystals. Our work elucidates that reaction precursors play an important role in in situ SC formation and stabilization, implying the possibility of applying this knowledge to other NC reactions.","lang":"eng"}],"type":"conference","oa_version":"None","date_created":"2025-07-21T08:33:20Z","status":"public","author":[{"orcid":"0000-0002-6962-8598","full_name":"Lee, Seungho","last_name":"Lee","first_name":"Seungho","id":"BB243B88-D767-11E9-B658-BC13E6697425"},{"id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","first_name":"Daniel","full_name":"Balazs, Daniel","orcid":"0000-0001-7597-043X"},{"id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","first_name":"Sharona","last_name":"Horta","full_name":"Horta, Sharona"},{"full_name":"Rayaroth Puthiyaveettil, Aiswarya","last_name":"Rayaroth Puthiyaveettil","first_name":"Aiswarya","id":"8aceb01b-8972-11ed-ae7b-d5fe53775add"},{"full_name":"Ibáñez, Maria","orcid":"0000-0001-5013-2843","first_name":"Maria","last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87"}],"title":"Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case","_id":"20055","language":[{"iso":"eng"}],"OA_type":"closed access","acknowledgement":"ISTA and the Werner Siemens Foundation financially supported this work. The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the Lab Support Facility (LSF).","publication":"Proceedings of the MATSUS Spring 2025 Conference"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"fulldoi":"https://doi.org/10.1038/s41590-025-02211-w","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","file_date_updated":"2025-07-31T08:00:33Z","date_published":"2025-08-01T00:00:00Z","intvolume":"        26","publication_status":"published","year":"2025","pmid":1,"quality_controlled":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"doi":"10.1038/s41590-025-02211-w","file":[{"date_created":"2025-07-31T08:00:33Z","checksum":"0c725123dca7797c682609bff2c4c5ac","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access","file_id":"20096","file_size":13514646,"date_updated":"2025-07-31T08:00:33Z","content_type":"application/pdf","file_name":"2025_NatureImmunology_ReisRodrigues.pdf"}],"volume":26,"isi":1,"publisher":"Springer Nature","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"month":"08","external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"corr_author":"1","oa":1,"day":"01","PlanS_conform":"1","date_updated":"2026-04-28T13:26:50Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"1258–1266","citation":{"mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>"},"project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","grant_number":"944-2020"}],"related_material":{"record":[{"id":"20149","relation":"dissertation_contains","status":"public"}],"link":[{"url":"https://ista.ac.at/en/news/bench-pressing-cells/","relation":"press_release","description":"News on ISTA website"}]},"abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","article_type":"letter_note","author":[{"id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues","first_name":"Patricia","orcid":"0000-0003-1681-508X","full_name":"Dos Reis Rodrigues, Patricia"},{"first_name":"Mario","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","orcid":"0000-0001-6406-524X","full_name":"Avellaneda Sarrió, Mario"},{"full_name":"Canigova, Nikola","orcid":"0000-0002-8518-5926","last_name":"Canigova","first_name":"Nikola","id":"3795523E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","first_name":"Florian R"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","first_name":"Kari","last_name":"Vaahtomeri","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518"},{"first_name":"Michael","last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311"},{"last_name":"De Vries","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid"},{"first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Yoshinori","last_name":"Fukui","full_name":"Fukui, Yoshinori"},{"id":"40F05888-F248-11E8-B48F-1D18A9856A87","last_name":"Juanes Garcia","first_name":"Alba","orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"date_created":"2025-07-27T22:01:26Z","status":"public","title":"Migrating immune cells globally coordinate protrusive forces","_id":"20082","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"scopus_import":"1","publication":"Nature Immunology"},{"ddc":["020"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"author":[{"full_name":"Gredler, Paul","last_name":"Gredler","first_name":"Paul"},{"first_name":"Christian","last_name":"Kaier","full_name":"Kaier, Christian"},{"id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","last_name":"Danowski","first_name":"Patrick","orcid":"0000-0002-6026-4409","full_name":"Danowski, Patrick"},{"last_name":"Zoyer","first_name":"Michael","full_name":"Zoyer, Michael"},{"last_name":"Rieck","first_name":"Katharina","full_name":"Rieck, Katharina"},{"last_name":"Ferus","first_name":"Andreas","full_name":"Ferus, Andreas"},{"full_name":"Rosenberger, Elisabeth","first_name":"Elisabeth","last_name":"Rosenberger"},{"full_name":"Löffler, Alexander","first_name":"Alexander","last_name":"Löffler"},{"first_name":"Lisa","last_name":"Hofer","full_name":"Hofer, Lisa"},{"last_name":"Still","first_name":"Laura","full_name":"Still, Laura"}],"status":"public","date_created":"2025-08-07T11:10:14Z","title":"Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures","_id":"20146","abstract":[{"lang":"eng","text":"This criteria catalogue and the accompanying assessment questions were developed by a working group of KEMÖ (Kooperation E-Medien Österreich, the Austrian Academic Library Consortium). They are intended to support research institutions and organisations in the evaluation of Open Science Infrastructures. The 20 criteria outlined in the catalogue provide a structured basis for making informed decisions regarding the financial support of these infrastructures.\r\n\r\nThe assessment questions are intended to be completed by Open Science Infrastructures and can be shared with them accordingly."}],"oa_version":"Published Version","type":"working_paper","publication_status":"published","year":"2025","citation":{"chicago":"Gredler, Paul, Christian Kaier, Patrick Danowski, Michael Zoyer, Katharina Rieck, Andreas Ferus, Elisabeth Rosenberger, Alexander Löffler, Lisa Hofer, and Laura Still. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo, 2025. <a href=\"https://doi.org/10.5281/zenodo.15269364\">https://doi.org/10.5281/zenodo.15269364</a>.","apa":"Gredler, P., Kaier, C., Danowski, P., Zoyer, M., Rieck, K., Ferus, A., … Still, L. (2025). <i>Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures</i>. Zenodo. <a href=\"https://doi.org/10.5281/zenodo.15269364\">https://doi.org/10.5281/zenodo.15269364</a>","ama":"Gredler P, Kaier C, Danowski P, et al. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo; 2025. doi:<a href=\"https://doi.org/10.5281/zenodo.15269364\">10.5281/zenodo.15269364</a>","mla":"Gredler, Paul, et al. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo, 2025, doi:<a href=\"https://doi.org/10.5281/zenodo.15269364\">10.5281/zenodo.15269364</a>.","ieee":"P. Gredler <i>et al.</i>, <i>Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures</i>. Zenodo, 2025.","short":"P. Gredler, C. Kaier, P. Danowski, M. Zoyer, K. Rieck, A. Ferus, E. Rosenberger, A. Löffler, L. Hofer, L. Still, Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures, Zenodo, 2025.","ista":"Gredler P, Kaier C, Danowski P, Zoyer M, Rieck K, Ferus A, Rosenberger E, Löffler A, Hofer L, Still L. 2025. Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures, Zenodo,p."},"article_processing_charge":"No","has_accepted_license":"1","date_published":"2025-08-07T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.5281/zenodo.15269364"}],"date_updated":"2025-08-11T07:20:03Z","fulldoi":"https://doi.org/10.5281/zenodo.15269364","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"day":"07","publisher":"Zenodo","department":[{"_id":"E-Lib"}],"month":"08","OA_type":"gold","OA_place":"publisher","doi":"10.5281/zenodo.15269364","language":[{"iso":"eng"}]},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-12-30T09:31:25Z","PlanS_conform":"1","project":[{"grant_number":"949120","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","call_identifier":"H2020","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"}],"citation":{"ama":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge and work function in scanning Kelvin probe microscopy. <i>Advanced Materials Interfaces</i>. 2025;12(19). doi:<a href=\"https://doi.org/10.1002/admi.202500521\">10.1002/admi.202500521</a>","mla":"Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” <i>Advanced Materials Interfaces</i>, vol. 12, no. 19, e00521, Wiley, 2025, doi:<a href=\"https://doi.org/10.1002/admi.202500521\">10.1002/admi.202500521</a>.","short":"I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials Interfaces 12 (2025).","ieee":"I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between surface charge and work function in scanning Kelvin probe microscopy,” <i>Advanced Materials Interfaces</i>, vol. 12, no. 19. Wiley, 2025.","ista":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 12(19), e00521.","chicago":"Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” <i>Advanced Materials Interfaces</i>. Wiley, 2025. <a href=\"https://doi.org/10.1002/admi.202500521\">https://doi.org/10.1002/admi.202500521</a>.","apa":"Lenton, I. C., Pertl, F., Shafeek, L. B., &#38; Waitukaitis, S. R. (2025). A duality between surface charge and work function in scanning Kelvin probe microscopy. <i>Advanced Materials Interfaces</i>. Wiley. <a href=\"https://doi.org/10.1002/admi.202500521\">https://doi.org/10.1002/admi.202500521</a>"},"DOAJ_listed":"1","article_type":"original","type":"journal_article","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic imaging of the electrostatic potential above a surface. Though most often used to image work-function variations of conductive surfaces, it can also be used to probe the surface charge on insulating surfaces. In both cases, relating the measured potential to the underlying signal is non-trivial. Here, general relationships are derived between the measured SKPM voltage and the underlying source, revealing either can be cast as a convolution with an appropriately scaled point spread function (PSF). For charge that exists on a thin insulating layer above a conductor, the PSF has the same shape as what would occur from a work-function variation alone, differing by a simple scaling factor. This relationship is confirmed by: (1) backing it out from finite-element simulations of work-function and charge signals, and (2) experimentally comparing the measured PSF from a small work-function target to that from a small charge spot. This scaling factor is further validated by comparing SKPM charge measurements with Faraday cup measurements for highly charged samples from contact-charging experiments. These results highlight a heretofore unappreciated connection between SKPM voltage and charge signals, offering a rigorous recipe to extract either from experimental data."}],"_id":"20295","title":"A duality between surface charge and work function in scanning Kelvin probe microscopy","date_created":"2025-09-07T22:01:33Z","author":[{"orcid":"0000-0002-5010-6984","full_name":"Lenton, Isaac C","first_name":"Isaac C","last_name":"Lenton","id":"a550210f-223c-11ec-8182-e2d45e817efb"},{"id":"6313aec0-15b2-11ec-abd3-ed67d16139af","first_name":"Felix","last_name":"Pertl","orcid":"0000-0003-0463-5794","full_name":"Pertl, Felix"},{"id":"3CD37A82-F248-11E8-B48F-1D18A9856A87","first_name":"Lubuna B","last_name":"Shafeek","full_name":"Shafeek, Lubuna B","orcid":"0000-0001-7180-6050"},{"last_name":"Waitukaitis","first_name":"Scott R","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176"}],"status":"public","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","publication_identifier":{"eissn":["2196-7350"]},"acknowledgement":"This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant agreement No. 949120). This research was supported by the Scientific Service Units of The Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions of the whole Waitukaitis group for useful discussions and feedback.","ec_funded":1,"publication":"Advanced Materials Interfaces","issue":"19","scopus_import":"1","fulldoi":"https://doi.org/10.1002/admi.202500521","arxiv":1,"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"date_published":"2025-10-01T00:00:00Z","file_date_updated":"2025-12-30T09:31:11Z","has_accepted_license":"1","article_processing_charge":"Yes","quality_controlled":"1","year":"2025","publication_status":"published","intvolume":"        12","ddc":["530"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"volume":12,"file":[{"file_id":"20908","file_size":1830117,"file_name":"2025_AdvMaterialsInterfaces_Lenton.pdf","content_type":"application/pdf","date_updated":"2025-12-30T09:31:11Z","date_created":"2025-12-30T09:31:11Z","checksum":"906fcc7733be8ce8a83600427b82cd5a","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access"}],"doi":"10.1002/admi.202500521","article_number":"e00521","isi":1,"external_id":{"isi":["001560163400001"],"arxiv":["2506.07187"]},"department":[{"_id":"ScWa"},{"_id":"NanoFab"}],"month":"10","publisher":"Wiley","day":"01","oa":1,"corr_author":"1"},{"intvolume":"       147","year":"2025","quality_controlled":"1","pmid":1,"publication_status":"published","ddc":["540"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"fulldoi":"https://doi.org/10.1021/jacs.5c09057","article_processing_charge":"Yes (via OA deal)","file_date_updated":"2025-09-10T07:53:10Z","date_published":"2025-08-01T00:00:00Z","has_accepted_license":"1","department":[{"_id":"PaSc"},{"_id":"NMR"}],"month":"08","publisher":"American Chemical Society","external_id":{"isi":["001542746200001"],"pmid":["40748291"]},"oa":1,"corr_author":"1","day":"01","file":[{"checksum":"b350d56ddddefea96cebd62c277c0ff5","date_created":"2025-09-10T07:53:10Z","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access","file_id":"20337","file_size":5235353,"date_updated":"2025-09-10T07:53:10Z","content_type":"application/pdf","file_name":"2025_JACS_Tatman.pdf"}],"doi":"10.1021/jacs.5c09057","volume":147,"isi":1,"abstract":[{"text":"Microsecond-to-millisecond motions are instrumental for many biomolecular functions, including enzymatic activity and ligand binding. Bloch-McConnell Relaxation Dispersion (BMRD) Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique for studying these dynamic processes. While BMRD experiments are routinely used to probe protein motions in solution, the experiment is more demanding in the solid state, where dipolar couplings complicate the spin dynamics. It is believed that high deuteration levels are required and sufficient to obtain accurate and quantitative data. Here we show that even under fast magic-angle spinning and high levels of deuteration artifactual “bumps” in 15N R1ρ BMRD profiles are common. The origin of these artifacts is identified as a second-order three-spin Mixed Rotational and Rotary Resonance (MIRROR) recoupling condition. These artifacts are found to be a significant confounding factor for the accurate quantification of microsecond protein dynamics using BMRD in the solid state. We show that the application of low-power continuous wave (CW) decoupling simultaneously with the 15N spin-lock leads to the suppression of these conditions and enables quantitative measurements of microsecond exchange in the solid state. Remarkably, the application of decoupling allows the measurement of accurate BMRD even in fully protonated proteins at 100 kHz MAS, thus extending the scope of μs dynamics measurements in MAS NMR.","lang":"eng"}],"article_type":"original","type":"journal_article","oa_version":"Published Version","author":[{"full_name":"Tatman, Benjamin","last_name":"Tatman","first_name":"Benjamin","id":"71cda2f3-e604-11ee-a1df-da10587eda3f"},{"full_name":"Sridharan, Vidhyalakshmi","first_name":"Vidhyalakshmi","last_name":"Sridharan"},{"first_name":"Motilal","last_name":"Uttarkabat","full_name":"Uttarkabat, Motilal"},{"last_name":"Jaroniec","first_name":"Christopher P.","full_name":"Jaroniec, Christopher P."},{"full_name":"Ernst, Matthias","last_name":"Ernst","first_name":"Matthias"},{"full_name":"Rovo, Petra","orcid":"0000-0001-8729-7326","id":"c316e53f-b965-11eb-b128-bb26acc59c00","first_name":"Petra","last_name":"Rovo"},{"orcid":"0000-0002-9350-7606","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda","first_name":"Paul"}],"status":"public","date_created":"2025-09-10T05:37:19Z","_id":"20321","title":"Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR","PlanS_conform":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-06-10T08:33:41Z","page":"29315-29326","citation":{"chicago":"Tatman, Benjamin, Vidhyalakshmi Sridharan, Motilal Uttarkabat, Christopher P. Jaroniec, Matthias Ernst, Petra Rovo, and Paul Schanda. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2025. <a href=\"https://doi.org/10.1021/jacs.5c09057\">https://doi.org/10.1021/jacs.5c09057</a>.","apa":"Tatman, B., Sridharan, V., Uttarkabat, M., Jaroniec, C. P., Ernst, M., Rovo, P., &#38; Schanda, P. (2025). Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.5c09057\">https://doi.org/10.1021/jacs.5c09057</a>","mla":"Tatman, Benjamin, et al. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” <i>Journal of the American Chemical Society</i>, vol. 147, no. 32, American Chemical Society, 2025, pp. 29315–26, doi:<a href=\"https://doi.org/10.1021/jacs.5c09057\">10.1021/jacs.5c09057</a>.","ama":"Tatman B, Sridharan V, Uttarkabat M, et al. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. <i>Journal of the American Chemical Society</i>. 2025;147(32):29315-29326. doi:<a href=\"https://doi.org/10.1021/jacs.5c09057\">10.1021/jacs.5c09057</a>","ieee":"B. Tatman <i>et al.</i>, “Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR,” <i>Journal of the American Chemical Society</i>, vol. 147, no. 32. American Chemical Society, pp. 29315–29326, 2025.","ista":"Tatman B, Sridharan V, Uttarkabat M, Jaroniec CP, Ernst M, Rovo P, Schanda P. 2025. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. Journal of the American Chemical Society. 147(32), 29315–29326.","short":"B. Tatman, V. Sridharan, M. Uttarkabat, C.P. Jaroniec, M. Ernst, P. Rovo, P. Schanda, Journal of the American Chemical Society 147 (2025) 29315–29326."},"related_material":{"record":[{"status":"public","relation":"used_in_publication","id":"19696"}]},"publication_identifier":{"issn":["0002-7863"],"eissn":["1520-5126"]},"acknowledgement":"The authors thank Alexey Krushelnitsky for useful discussions. C.P.J. thanks NSF (MCB-2303862) and NIH (R35GM156238 and S10OD012303) for funding. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities.","issue":"32","scopus_import":"1","publication":"Journal of the American Chemical Society","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"hybrid"},{"fulldoi":"https://doi.org/10.1021/jacs.5c11435","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"NanoFab"},{"_id":"MassSpec"}],"has_accepted_license":"1","date_published":"2025-08-22T00:00:00Z","file_date_updated":"2025-09-10T06:55:17Z","article_processing_charge":"Yes (via OA deal)","publication_status":"published","quality_controlled":"1","year":"2025","intvolume":"       147","ddc":["540"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"volume":147,"doi":"10.1021/jacs.5c11435","file":[{"relation":"main_file","creator":"dernst","access_level":"open_access","date_created":"2025-09-10T06:55:17Z","checksum":"52892fa91adadd39a1c42da9e01139a5","success":1,"file_size":9997327,"date_updated":"2025-09-10T06:55:17Z","content_type":"application/pdf","file_name":"2025_JACS_Liu.pdf","file_id":"20334"}],"isi":1,"external_id":{"isi":["001558320100001"]},"publisher":"American Chemical Society","month":"08","department":[{"_id":"MaIb"},{"_id":"MassSpec"}],"day":"22","corr_author":"1","oa":1,"date_updated":"2026-07-28T09:55:14Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","related_material":{"record":[{"relation":"dissertation_contains","id":"22017","status":"public"}]},"page":"32199-32208","citation":{"short":"Y. Liu, T. Kleinhanns, S. Horta, E. Dutkiewicz, S. Lu, M.C. Spadaro, A. Genç, L. Chen, K.H. Lim, M. Hong, J. Arbiol, M. Ibáñez, Journal of the American Chemical Society 147 (2025) 32199–32208.","ista":"Liu Y, Kleinhanns T, Horta S, Dutkiewicz E, Lu S, Spadaro MC, Genç A, Chen L, Lim KH, Hong M, Arbiol J, Ibáñez M. 2025. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical Society. 147(35), 32199–32208.","ieee":"Y. Liu <i>et al.</i>, “Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se,” <i>Journal of the American Chemical Society</i>, vol. 147, no. 35. American Chemical Society, pp. 32199–32208, 2025.","mla":"Liu, Yu, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” <i>Journal of the American Chemical Society</i>, vol. 147, no. 35, American Chemical Society, 2025, pp. 32199–208, doi:<a href=\"https://doi.org/10.1021/jacs.5c11435\">10.1021/jacs.5c11435</a>.","ama":"Liu Y, Kleinhanns T, Horta S, et al. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. <i>Journal of the American Chemical Society</i>. 2025;147(35):32199-32208. doi:<a href=\"https://doi.org/10.1021/jacs.5c11435\">10.1021/jacs.5c11435</a>","apa":"Liu, Y., Kleinhanns, T., Horta, S., Dutkiewicz, E., Lu, S., Spadaro, M. C., … Ibáñez, M. (2025). Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.5c11435\">https://doi.org/10.1021/jacs.5c11435</a>","chicago":"Liu, Yu, Tobias Kleinhanns, Sharona Horta, Ewelina Dutkiewicz, Shaoqing Lu, Maria Chiara Spadaro, Aziz Genç, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2025. <a href=\"https://doi.org/10.1021/jacs.5c11435\">https://doi.org/10.1021/jacs.5c11435</a>."},"project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"oa_version":"Published Version","type":"journal_article","article_type":"original","abstract":[{"lang":"eng","text":"Ag2Se is a promising n-type thermoelectric material, but its performance is limited by excessive carrier concentration, compositional inhomogeneity, and phase instability, challenges rooted in a narrow homogeneity range and uncontrolled Ag+ diffusion in the superionic phase. Here, we address these issues by exploiting liquid–solid interface reactions using CdSe complexes that remove surface excess Ag to yield stoichiometric Ag2Se and generate CdSe nanodomains that inhibit Ag+ diffusion and constrain grain growth. The resulting Ag2Se-CdSe nanocomposites exhibit a reproducible, stable figure of merit (zT) of 1.04 between 300 and 390 K. Beyond demonstrating high performance, we elucidate the interfacial chemical reactions that give rise to the observed microstructure and transport properties, providing a foundation for rationally engineering interfacial chemistry to tailor transport properties across diverse thermoelectric material systems."}],"title":"Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se","_id":"20326","author":[{"last_name":"Liu","first_name":"Yu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7313-6740","full_name":"Liu, Yu"},{"orcid":"0000-0003-1537-7436","full_name":"Kleinhanns, Tobias","id":"8BD9DE16-AB3C-11E9-9C8C-2A03E6697425","first_name":"Tobias","last_name":"Kleinhanns"},{"id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","last_name":"Horta","first_name":"Sharona","full_name":"Horta, Sharona"},{"full_name":"Dutkiewicz, Ewelina","first_name":"Ewelina","last_name":"Dutkiewicz","id":"0601cc46-c082-11ec-9b07-bb29641d1de9"},{"full_name":"Lu, Shaoqing","last_name":"Lu","first_name":"Shaoqing"},{"full_name":"Spadaro, Maria Chiara","first_name":"Maria Chiara","last_name":"Spadaro"},{"full_name":"Genç, Aziz","last_name":"Genç","first_name":"Aziz"},{"last_name":"Chen","first_name":"Lei","full_name":"Chen, Lei"},{"first_name":"Khak Ho","last_name":"Lim","full_name":"Lim, Khak Ho"},{"full_name":"Hong, Min","first_name":"Min","last_name":"Hong"},{"last_name":"Arbiol","first_name":"Jordi","full_name":"Arbiol, Jordi"},{"orcid":"0000-0001-5013-2843","full_name":"Ibáñez, Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","last_name":"Ibáñez"}],"date_created":"2025-09-10T05:44:03Z","status":"public","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","acknowledgement":"M.I. acknowledges financial support from ISTA and the Werner Siemens Foundation. The Scientific Service Units (SSU) of ISTA supported this work through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF) and the Nanofabrication Facility (NNF) and the LSF Mass Spectrometry Service. The members of the Ibáñez research group are acknowledged, especially Christine Fiedler for scientific illustration and Ihor Cherniukh for valuable discussions. Y.L. acknowledges funding from the National Natural Science Foundation of China (NSFC) (Grants No. 22209034), the Innovation and Entrepreneurship Project of Overseas Returnees in Anhui Province (Grant No. 2022LCX002) and the Fundamental Research Funds for the Central Universities (JZ2024HGTB0239). K.H.L. acknowledges financial support from the National Natural Science Foundation of China (NSFC) (Grant No. 22208293). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. Authors acknowledge the Advanced Materials programme by the Spanish Government with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat de Catalunya (Project In-CAEM). The authors thank support from the project AMaDE (PID2023-149158OB-C43), funded by MCIN/AEI/10.13039/501100011033/and by “ERDF Away of making Europe”, by the “European Union”. ICN2 is supported by the Severo Ochoa program from Spanish MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat de Catalunya. ICN2 is founding member of e-DREAM. (68) M.H. acknowledges the funding from the Australian Research Council (FT230100316 and IH200100035). M.H. acknowledges the computational support from the National Computational Infrastructure (NCI) and Pawsey Supercomputing Centre, Australia.","publication_identifier":{"eissn":["1520-5126"],"issn":["0002-7863"]},"publication":"Journal of the American Chemical Society","scopus_import":"1","issue":"35"},{"author":[{"id":"4B807D68-AE37-11E9-AC72-31CAE5697425","first_name":"Juan Carlos A","last_name":"Sobarzo Ponce","full_name":"Sobarzo Ponce, Juan Carlos A"},{"id":"6313aec0-15b2-11ec-abd3-ed67d16139af","first_name":"Felix","last_name":"Pertl","full_name":"Pertl, Felix","orcid":"0000-0003-0463-5794"},{"orcid":"0000-0001-7597-043X","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","first_name":"Daniel"},{"full_name":"Costanzo, Tommaso","orcid":"0000-0001-9732-3815","first_name":"Tommaso","last_name":"Costanzo","id":"D93824F4-D9BA-11E9-BB12-F207E6697425"},{"last_name":"Sauer","first_name":"Markus","full_name":"Sauer, Markus"},{"full_name":"Foelske, Annette","last_name":"Foelske","first_name":"Annette"},{"last_name":"Ostermann","first_name":"Markus","full_name":"Ostermann, Markus"},{"last_name":"Pichler","first_name":"Christian M.","full_name":"Pichler, Christian M."},{"full_name":"Wang, Yongkang","first_name":"Yongkang","last_name":"Wang"},{"full_name":"Nagata, Yuki","first_name":"Yuki","last_name":"Nagata"},{"full_name":"Bonn, Mischa","last_name":"Bonn","first_name":"Mischa"},{"last_name":"Waitukaitis","first_name":"Scott R","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176"}],"status":"public","date_created":"2025-03-02T23:01:52Z","_id":"19278","title":"Spontaneous ordering of identical materials into a triboelectric series","abstract":[{"lang":"eng","text":"When two insulating, neutral materials are contacted and separated, they exchange electrical charge1. Experiments have long suggested that this ‘contact electrification’ is transitive, with different materials ordering into ‘triboelectric series’ based on the sign of charge acquired2. At the same time, the effect is plagued by unpredictability, preventing consensus on the mechanism and casting doubt on the rhyme and reason that series imply3. Here we expose an unanticipated connection between the unpredictability and order in contact electrification: nominally identical materials initially exchange charge randomly and intransitively, but—over repeated experiments—order into triboelectric series. We find that this evolution is driven by the act of contact itself—samples with more contacts in their history charge negatively to ones with fewer contacts. Capturing this ‘contact bias’ in a minimal model, we recreate both the initial randomness and ultimate order in numerical simulations and use it experimentally to force the appearance of a triboelectric series of our choosing. With a set of surface-sensitive techniques to search for the underlying alterations contact creates, we only find evidence of nanoscale morphological changes, pointing to a mechanism strongly coupled with mechanics. Our results highlight the centrality of contact history in contact electrification and suggest that focusing on the unpredictability that has long plagued the effect may hold the key to understanding it."}],"article_type":"original","oa_version":"Published Version","type":"journal_article","project":[{"grant_number":"949120","name":"Tribocharge: a multi-scale approach to an enduring problem in physics","call_identifier":"H2020","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa"}],"citation":{"mla":"Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>, vol. 638, no. 8051, 664–669, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>.","ama":"Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. 2025;638(8051). doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>","ista":"Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering of identical materials into a triboelectric series. Nature. 638(8051), 664–669.","ieee":"J. C. A. Sobarzo Ponce <i>et al.</i>, “Spontaneous ordering of identical materials into a triboelectric series,” <i>Nature</i>, vol. 638, no. 8051. Springer Nature, 2025.","short":"J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske, M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature 638 (2025).","chicago":"Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo, Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>.","apa":"Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske, A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>"},"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20203"},{"status":"public","relation":"dissertation_contains","id":"22684"}],"link":[{"url":"https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/","relation":"press_release","description":"News on ISTA website"}]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-08-27T11:42:43Z","issue":"8051","scopus_import":"1","publication":"Nature","acknowledgement":"This project has received financing from the European Research Council grant agreement no. 949120 under the European Union’s Horizon 2020 research and innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility, Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez and G. Anciaux for the suggestion to look into the roughness power spectral density. We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements. Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"ec_funded":1,"OA_place":"publisher","OA_type":"hybrid","language":[{"iso":"eng"}],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["530"],"intvolume":"       638","quality_controlled":"1","pmid":1,"year":"2025","publication_status":"published","article_processing_charge":"Yes (via OA deal)","file_date_updated":"2025-03-04T10:05:18Z","date_published":"2025-02-20T00:00:00Z","has_accepted_license":"1","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"fulldoi":"https://doi.org/10.1038/s41586-024-08530-6","corr_author":"1","oa":1,"day":"20","department":[{"_id":"ScWa"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"month":"02","publisher":"Springer Nature","external_id":{"isi":["001428076100015"],"pmid":["39972227"]},"article_number":"664-669","isi":1,"file":[{"file_id":"19289","date_updated":"2025-03-04T10:05:18Z","content_type":"application/pdf","file_name":"2025_Nature_Sobarzo.pdf","file_size":3807415,"success":1,"date_created":"2025-03-04T10:05:18Z","checksum":"fecf302274dd3218d3e7dd22f39a6c0c","access_level":"open_access","relation":"main_file","creator":"dernst"}],"doi":"10.1038/s41586-024-08530-6","volume":638},{"OA_type":"hybrid","OA_place":"publisher","language":[{"iso":"eng"}],"scopus_import":"1","issue":"8085","publication":"Nature","acknowledgement":"S.A.F. thanks the Institute of Science and Technology Austria (ISTA) for the support. The Scientific Service Units of ISTA supported this research through resources provided by the Imaging and Optics Facility, the Lab Support Facility, the Miba Machine Shop and Scientific Computing. This research was partly funded by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open access purposes, the author has applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. R.H. acknowledges funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989). H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life and R. Jethwa for proofreading. Open access funding was provided by ISTA.","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"page":"601–605","citation":{"short":"S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025) 601–605.","ieee":"S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics control singlet and triplet oxygen evolving from superoxide,” <i>Nature</i>, vol. 646, no. 8085. Springer Nature, pp. 601–605, 2025.","ista":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605.","mla":"Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>, vol. 646, no. 8085, Springer Nature, 2025, pp. 601–605, doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>.","ama":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. 2025;646(8085):601–605. doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>","apa":"Mondal, S., Nguyen, H. T. K., Hauschild, R., &#38; Freunberger, S. A. (2025). Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>","chicago":"Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>."},"project":[{"grant_number":"P37169","_id":"8df062be-16d5-11f0-9cad-f559b6612c7e","name":"Singlet oxygen in non-aqueous oxygen redox chemistry"},{"grant_number":"CZI01","name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"name":"Materials for Energy Conversion and Storage (Freunberger)","_id":"5eaf4378-b033-11f1-b276-f928018a46c1","grant_number":"COE05"}],"related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/taming-the-bad-oxygen/"}]},"PlanS_conform":"1","date_updated":"2026-09-16T06:53:54Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Mondal","first_name":"Soumyadip","id":"d25d21ef-dc8d-11ea-abe3-ec4576307f48","full_name":"Mondal, Soumyadip"},{"last_name":"Nguyen","first_name":"Huyen T.K.","full_name":"Nguyen, Huyen T.K."},{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Stefan Alexander","last_name":"Freunberger","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","full_name":"Freunberger, Stefan Alexander","orcid":"0000-0003-2902-5319"}],"date_created":"2024-08-29T10:40:23Z","status":"public","title":"Marcus kinetics control singlet and triplet oxygen evolving from superoxide","_id":"17468","abstract":[{"text":"Oxygen redox chemistry is central to life1 and many human-made technologies, such as in energy storage2,3,4. The large energy gain from oxygen redox reactions is often connected with the occurrence of harmful reactive oxygen species3,5,6. Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which may evolve from superoxide. However, the factors determining the formation of singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown. Here we report that the release of triplet or singlet oxygen is governed by individual Marcus normal and inverted region behaviour. We found that as the driving force for the reaction increases, the initially dominant evolution of triplet oxygen slows down, and singlet oxygen evolution becomes predominant with higher maximum kinetics. This behaviour also applies to the widely observed superoxide disproportionation, in which one superoxide is oxidized by another, in both non-aqueous and aqueous systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet oxygen yields governed by these conditions are relevant, for example, in batteries or cellular organelles in which superoxide forms. Our findings suggest ways to understand and control spin states and kinetics in oxygen redox chemistry, with implications for fields, including life sciences, pure chemistry and energy storage.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","article_type":"original","isi":1,"doi":"10.1038/s41586-025-09587-7","file":[{"date_created":"2025-10-20T10:26:13Z","checksum":"b507ddd23df0388aa65d04dc9b00fe3d","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access","file_id":"20500","file_size":3809247,"file_name":"2025_Nature_Mondal.pdf","date_updated":"2025-10-20T10:26:13Z","content_type":"application/pdf"}],"volume":646,"corr_author":"1","oa":1,"day":"16","publisher":"Springer Nature","department":[{"_id":"StFr"},{"_id":"Bio"}],"month":"10","external_id":{"pmid":["41044415"],"isi":["001586378900001"]},"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","date_published":"2025-10-16T00:00:00Z","file_date_updated":"2025-10-20T10:26:13Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"fulldoi":"https://doi.org/10.1038/s41586-025-09587-7","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["540"],"intvolume":"       646","publication_status":"published","quality_controlled":"1","year":"2025","pmid":1},{"abstract":[{"lang":"eng","text":"Research involving human subjects or identifiable human material and data must be assessed by an ethics committee. The Karl Landsteiner University of Health Sciences has established a Commission on Ethics and Scientific Integrity to evaluate medical research conducted by its faculty and students and at its affiliated hospitals.\r\nAll projects submitted to the Commission on Ethics and Scientific Integrity between 2018 and 2023 were analyzed regarding their major characteristics, the duration of the evaluation process, and votes issued.\r\nA total of 520 applications were electronically submitted during the observation period. Most of the studies were retrospective data analyses in the field of oncology, psychology and surgery. Most studies included less than 100 volunteers. Of the applications 50% received a final vote within 5 months, during which several revision rounds took place. Overall, about 77% of votes issued during the observation period were positive and 2% were rejections. In 11% files were closed due to withdrawal. In 11% final votes were pending at the end of the observation period due to requests for revisions.\r\nOur results emphasize the importance of institutional ethics committees using the example of the Commission on Ethics and Scientific Integrity at the Karl Landsteiner University. Such committees fill a gap in evaluating research not covered by Austrian legal regulations. Continuous development of standards, operating procedures, and national and international collaborations are required to assess and minimize risks to trial subjects and to provide a safe and productive environment for research in human medicine and related fields."}],"article_type":"original","type":"journal_article","oa_version":"Published Version","author":[{"full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","last_name":"Schober","first_name":"Sophie"},{"first_name":"Sascha","last_name":"Klee","full_name":"Klee, Sascha"},{"last_name":"Trautinger","first_name":"Franz","full_name":"Trautinger, Franz"}],"date_created":"2024-10-20T22:02:07Z","status":"public","_id":"18449","title":"The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023","PlanS_conform":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","supplementarymaterial":"no","date_updated":"2026-09-17T10:27:37Z","page":"432-437","citation":{"short":"S. Schober, S. Klee, F. Trautinger, Wiener Klinische Wochenschrift 137 (2025) 432–437.","ieee":"S. Schober, S. Klee, and F. Trautinger, “The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023,” <i>Wiener Klinische Wochenschrift</i>, vol. 137. Springer Nature, pp. 432–437, 2025.","ista":"Schober S, Klee S, Trautinger F. 2025. The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische Wochenschrift. 137, 432–437.","ama":"Schober S, Klee S, Trautinger F. The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. <i>Wiener Klinische Wochenschrift</i>. 2025;137:432-437. doi:<a href=\"https://doi.org/10.1007/s00508-024-02462-x\">10.1007/s00508-024-02462-x</a>","mla":"Schober, Sophie, et al. “The Role of Institutional Ethics Committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023.” <i>Wiener Klinische Wochenschrift</i>, vol. 137, Springer Nature, 2025, pp. 432–37, doi:<a href=\"https://doi.org/10.1007/s00508-024-02462-x\">10.1007/s00508-024-02462-x</a>.","apa":"Schober, S., Klee, S., &#38; Trautinger, F. (2025). The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. <i>Wiener Klinische Wochenschrift</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s00508-024-02462-x\">https://doi.org/10.1007/s00508-024-02462-x</a>","chicago":"Schober, Sophie, Sascha Klee, and Franz Trautinger. “The Role of Institutional Ethics Committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023.” <i>Wiener Klinische Wochenschrift</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1007/s00508-024-02462-x\">https://doi.org/10.1007/s00508-024-02462-x</a>."},"acknowledgement":"Open access funding provided by Karl Landsteiner University.","publication_identifier":{"issn":["0043-5325"],"eissn":["1613-7671"]},"scopus_import":"1","publication":"Wiener Klinische Wochenschrift","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","intvolume":"       137","quality_controlled":"1","year":"2025","publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"fulldoi":"https://doi.org/10.1007/s00508-024-02462-x","das_tickbox":"0","article_processing_charge":"Yes (via OA deal)","file_date_updated":"2025-12-30T06:54:03Z","date_published":"2025-07-01T00:00:00Z","has_accepted_license":"1","department":[{"_id":"PreCl"}],"month":"07","publisher":"Springer Nature","external_id":{"isi":["001329812000001"]},"oa":1,"corr_author":"1","day":"01","file":[{"date_updated":"2025-12-30T06:54:03Z","content_type":"application/pdf","file_name":"2025_WrKlinischeWochenschrift_Schober.pdf","file_size":580791,"file_id":"20880","access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"date_created":"2025-12-30T06:54:03Z","checksum":"321be8a584117feaea9f3feaa28caabd"}],"researchdata_availability":"no","doi":"10.1007/s00508-024-02462-x","volume":137,"isi":1},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"PreCl"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"fulldoi":"https://doi.org/10.1038/s41586-025-08985-1","article_processing_charge":"Yes (via OA deal)","file_date_updated":"2025-07-03T06:55:20Z","date_published":"2025-06-12T00:00:00Z","has_accepted_license":"1","intvolume":"       642","pmid":1,"year":"2025","quality_controlled":"1","publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"file":[{"access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"checksum":"ebc99d7108e728f46db0a009292675ef","date_created":"2025-07-03T06:55:20Z","date_updated":"2025-07-03T06:55:20Z","content_type":"application/pdf","file_name":"2025_Nature_Tavakoli.pdf","file_size":133201290,"file_id":"19959"}],"doi":"10.1038/s41586-025-08985-1","volume":642,"isi":1,"month":"06","department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"GaNo"}],"publisher":"Springer Nature","external_id":{"isi":["001483477000001"],"pmid":["40335689"]},"corr_author":"1","oa":1,"day":"12","PlanS_conform":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-10-02T09:59:44Z","project":[{"grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy"},{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385"},{"_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders","grant_number":"101044865"},{"grant_number":"W1232-B24","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","call_identifier":"FWF"}],"page":"398-410","citation":{"mla":"Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>, vol. 642, Springer Nature, 2025, pp. 398–410, doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>.","ama":"Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. 2025;642:398-410. doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>","short":"M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas, J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino, V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.","ieee":"M. Tavakoli <i>et al.</i>, “Light-microscopy-based connectomic reconstruction of mammalian brain tissue,” <i>Nature</i>, vol. 642. Springer Nature, pp. 398–410, 2025.","ista":"Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 642, 398–410.","chicago":"Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>.","apa":"Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas, N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>"},"related_material":{"record":[{"relation":"research_data","id":"18697","status":"public"},{"status":"public","relation":"earlier_version","id":"18677"}],"link":[{"url":"https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/","description":"News on ISTA website","relation":"press_release"}]},"abstract":[{"lang":"eng","text":"The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction by light microscopy has been out of reach, owing to limitations in resolution, contrast and volumetric imaging capability. Here we describe light-microscopy-based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning-based segmentation and analysis of connectivity, thereby directly incorporating molecular information into synapse-level reconstructions of brain tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological experiments in a readily adoptable manner."}],"article_type":"original","type":"journal_article","oa_version":"Published Version","date_created":"2025-05-18T22:02:51Z","author":[{"first_name":"Mojtaba","last_name":"Tavakoli","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","last_name":"Lyudchik","first_name":"Julia","full_name":"Lyudchik, Julia"},{"full_name":"Januszewski, Michał","last_name":"Januszewski","first_name":"Michał"},{"full_name":"Vistunou, Vitali","first_name":"Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81"},{"full_name":"Agudelo Duenas, Nathalie","last_name":"Agudelo Duenas","first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","first_name":"Jakob","last_name":"Vorlaufer","full_name":"Vorlaufer, Jakob","orcid":"0009-0000-7590-3501"},{"full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","last_name":"Sommer"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","last_name":"Kreuzinger","first_name":"Caroline","full_name":"Kreuzinger, Caroline"},{"full_name":"Oliveira, Bárbara","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","last_name":"Oliveira","first_name":"Bárbara"},{"last_name":"Cenameri","first_name":"Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886","full_name":"Cenameri, Alban"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","first_name":"Gaia","last_name":"Novarino","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia"},{"full_name":"Jain, Viren","first_name":"Viren","last_name":"Jain"},{"first_name":"Johann G","last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973"}],"status":"public","_id":"19704","title":"Light-microscopy-based connectomic reconstruction of mammalian brain tissue","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","acknowledgement":"We thank S. Dorkenwald and P. Li for critical reading of the manuscript, S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open access funding provided by Institute of Science and Technology (IST Austria).","ec_funded":1,"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"scopus_import":"1","publication":"Nature"},{"project":[{"grant_number":"101069515","name":"Integrated Germanium Quantum Technology","_id":"34c0acea-11ca-11ed-8bc3-8775e10fd452"},{"grant_number":"P32235","_id":"237B3DA4-32DE-11EA-91FC-C7463DDC885E","call_identifier":"FWF","name":"Towards scalable hut wire quantum devices"},{"name":"High impedance circuit quantum electrodynamics with hole spins","_id":"c0977eea-5a5b-11eb-8a69-a862db0cf4d1","grant_number":"I05060"},{"grant_number":"P36507","name":"Merging spin and superconducting qubits in planar Ge","_id":"bd8bd29e-d553-11ed-ba76-f0070d4b237a"},{"call_identifier":"H2020","name":"IST-BRIDGE: International postdoctoral program","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413"},{"_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1","call_identifier":"FWF","name":"FWF Open Access Fund"}],"citation":{"short":"M. Janik, K.E.R. Roux, C.N. Borja Espinosa, O. Sagi, A. Baghdadi, T. Adletzberger, S. Calcaterra, M. Botifoll, A. Garzón Manjón, J. Arbiol, D. Chrastina, G. Isella, I.M. Pop, G. Katsaros, Nature Communications 16 (2025).","ieee":"M. Janik <i>et al.</i>, “Strong charge-photon coupling in planar germanium enabled by granular aluminium superinductors,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","ista":"Janik M, Roux KER, Borja Espinosa CN, Sagi O, Baghdadi A, Adletzberger T, Calcaterra S, Botifoll M, Garzón Manjón A, Arbiol J, Chrastina D, Isella G, Pop IM, Katsaros G. 2025. Strong charge-photon coupling in planar germanium enabled by granular aluminium superinductors. Nature Communications. 16, 2103.","ama":"Janik M, Roux KER, Borja Espinosa CN, et al. Strong charge-photon coupling in planar germanium enabled by granular aluminium superinductors. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-57252-4\">10.1038/s41467-025-57252-4</a>","mla":"Janik, Marian, et al. “Strong Charge-Photon Coupling in Planar Germanium Enabled by Granular Aluminium Superinductors.” <i>Nature Communications</i>, vol. 16, 2103, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-57252-4\">10.1038/s41467-025-57252-4</a>.","apa":"Janik, M., Roux, K. E. R., Borja Espinosa, C. N., Sagi, O., Baghdadi, A., Adletzberger, T., … Katsaros, G. (2025). Strong charge-photon coupling in planar germanium enabled by granular aluminium superinductors. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-57252-4\">https://doi.org/10.1038/s41467-025-57252-4</a>","chicago":"Janik, Marian, Kevin Etienne Robert Roux, Carla N Borja Espinosa, Oliver Sagi, Abdulhamid Baghdadi, Thomas Adletzberger, Stefano Calcaterra, et al. “Strong Charge-Photon Coupling in Planar Germanium Enabled by Granular Aluminium Superinductors.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-57252-4\">https://doi.org/10.1038/s41467-025-57252-4</a>."},"DOAJ_listed":"1","related_material":{"record":[{"status":"public","id":"18886","relation":"research_data"},{"id":"18144","relation":"earlier_version","status":"public"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-02T11:05:09Z","status":"public","date_created":"2025-03-16T23:01:23Z","author":[{"full_name":"Janik, Marian","orcid":"0009-0003-9037-8831","id":"396A1950-F248-11E8-B48F-1D18A9856A87","last_name":"Janik","first_name":"Marian"},{"id":"53f93ea2-803f-11ed-ab7e-b283135794ef","first_name":"Kevin Etienne Robert","last_name":"Roux","full_name":"Roux, Kevin Etienne Robert"},{"full_name":"Borja Espinosa, Carla N","first_name":"Carla N","last_name":"Borja Espinosa","id":"18777c01-896a-11ed-bdf8-e4851dc07d16"},{"full_name":"Sagi, Oliver","id":"71616374-A8E9-11E9-A7CA-09ECE5697425","first_name":"Oliver","last_name":"Sagi"},{"full_name":"Baghdadi, Abdulhamid","id":"160D87FA-96B5-11E9-BF77-7626E6697425","last_name":"Baghdadi","first_name":"Abdulhamid"},{"id":"38756BB2-F248-11E8-B48F-1D18A9856A87","first_name":"Thomas","last_name":"Adletzberger","full_name":"Adletzberger, Thomas"},{"full_name":"Calcaterra, Stefano","last_name":"Calcaterra","first_name":"Stefano"},{"full_name":"Botifoll, Marc","last_name":"Botifoll","first_name":"Marc"},{"full_name":"Garzón Manjón, Alba","last_name":"Garzón Manjón","first_name":"Alba"},{"full_name":"Arbiol, Jordi","first_name":"Jordi","last_name":"Arbiol"},{"full_name":"Chrastina, Daniel","last_name":"Chrastina","first_name":"Daniel"},{"full_name":"Isella, Giovanni","first_name":"Giovanni","last_name":"Isella"},{"full_name":"Pop, Ioan M.","last_name":"Pop","first_name":"Ioan M."},{"last_name":"Katsaros","first_name":"Georgios","id":"38DB5788-F248-11E8-B48F-1D18A9856A87","full_name":"Katsaros, Georgios","orcid":"0000-0001-8342-202X"}],"_id":"19401","title":"Strong charge-photon coupling in planar germanium enabled by granular aluminium superinductors","abstract":[{"lang":"eng","text":"High kinetic inductance superconductors are gaining increasing interest for the realisation of qubits, amplifiers and detectors. Moreover, thanks to their high impedance, quantum buses made of such materials enable large zero-point fluctuations of the voltage, boosting the coupling rates to spin and charge qubits. However, fully exploiting the potential of disordered or granular superconductors is challenging, as their inductance and, therefore, impedance at high values are difficult to control. Here, we report a reproducible fabrication of granular aluminium resonators by developing a wireless ohmmeter, which allows in situ measurements during film deposition and, therefore, control of the kinetic inductance of granular aluminium films. Reproducible fabrication of circuits with impedances (inductances) exceeding 13 kΩ (1 nH per square) is now possible. By integrating a 7.9 kΩ resonator with a germanium double quantum dot, we demonstrate strong charge-photon coupling with a rate of gc/2π = 566 ± 2 MHz. This broadly applicable method opens the path for novel qubits and high-fidelity, long-distance two-qubit gates."}],"article_type":"original","type":"journal_article","oa_version":"Published Version","OA_type":"gold","OA_place":"publisher","language":[{"iso":"eng"}],"scopus_import":"1","publication":"Nature Communications","ec_funded":1,"publication_identifier":{"eissn":["2041-1723"]},"acknowledgement":"We acknowledge Franco De Palma, Mahya Khorramshahi, Fabian Oppliger, Thomas Reisinger, Pasquale Scarlino and Xiao Xue for helpful discussions. We thank Simon Robson for proofreading the manuscript. This research was supported by the Scientific Service Units of ISTA through resources provided by the MIBA Machine Shop and the Nanofabrication facility. This research and related results were made possible with the support of the NOMIS Foundation and the HORIZON-RIA 101069515 project. This research was funded in whole or in part by the Austrian Science Fund (FWF) https://doi.org/10.55776/P32235, https://doi.org/10.55776/I5060 and https://doi.org/10.55776/P36507. For Open Access purposes, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission. M.J. acknowledges funding from FellowQUTE 2024-01. K.R. acknowledges funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 101034413. I.M.P. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG - German Research Foundation) under project number 450396347 (GeHoldeQED). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. We acknowledge support from CSIC Interdisciplinary Thematic Platform (PTI+) on Quantum Technologies (PTI-QTEP+). This research work has been funded by the European Commission - NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Quantum Technologies Platform (QTEP). ICN2 is supported by the Severo Ochoa programme from Spanish MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat de Catalunya. Part of the present work has been performed in the framework of Universitat Autònoma de Barcelona Materials Science PhD programme. AGM has received funding from Grant RYC2021-033479-I funded by MCIN/AEI/10.13039/501100011033 and by European Union NextGenerationEU/PRTR. M.B. acknowledges support from SUR Generalitat de Catalunya and the EU Social Fund; project ref. 2020 FI 00103. The authors acknowledge the use of instrumentation and the technical advice provided by the Joint Electron Microscopy Centre at ALBA (JEMCA). ICN2 acknowledges funding from Grant IU16-014206 (METCAM-FIB) funded by the European Union through the European Regional Development Fund (ERDF), with the support of the Ministry of Research and Universities, Generalitat de Catalunya. ICN2 is a founding member of e-DREAM60.","article_processing_charge":"Yes","file_date_updated":"2025-03-17T10:53:32Z","date_published":"2025-03-01T00:00:00Z","has_accepted_license":"1","arxiv":1,"APC_amount":"7068 EUR","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"}],"fulldoi":"https://doi.org/10.1038/s41467-025-57252-4","ddc":["530"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"intvolume":"        16","year":"2025","quality_controlled":"1","pmid":1,"publication_status":"published","article_number":"2103","isi":1,"file":[{"success":1,"date_created":"2025-03-17T10:53:32Z","checksum":"a9383dd978ca2c50b7dded6c0bb2cd49","access_level":"open_access","creator":"dernst","relation":"main_file","file_id":"19415","content_type":"application/pdf","date_updated":"2025-03-17T10:53:32Z","file_name":"2025_NatureComm_Janik.pdf","file_size":6364878}],"doi":"10.1038/s41467-025-57252-4","volume":16,"corr_author":"1","oa":1,"day":"01","month":"03","department":[{"_id":"GeKa"},{"_id":"JoFi"},{"_id":"M-Shop"}],"publisher":"Springer Nature","external_id":{"isi":["001434774800001"],"pmid":["40025007"],"arxiv":["2407.03079"]}},{"_id":"19404","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","author":[{"last_name":"Tavano","first_name":"Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9970-7804","full_name":"Tavano, Ste"},{"full_name":"Brückner, David","orcid":"0000-0001-7205-2975","first_name":"David","last_name":"Brückner","id":"e1e86031-6537-11eb-953a-f7ab92be508d"},{"first_name":"Saren","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren"},{"full_name":"Tong, Xin","last_name":"Tong","first_name":"Xin","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425"},{"full_name":"Kardos, Roland","last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-7659-9142","full_name":"Schauer, Alexandra","first_name":"Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"status":"public","date_created":"2025-03-16T23:01:24Z","article_type":"original","oa_version":"Published Version","type":"journal_article","abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"project":[{"name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022"},{"grant_number":"ALTF 1159-2018","_id":"269CD5C4-B435-11E9-9278-68D0E5697425","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration"}],"DOAJ_listed":"1","citation":{"ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Cell Press, 2025.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:20:45Z","publication":"Cell Reports","issue":"3","scopus_import":"1","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","OA_place":"publisher","OA_type":"gold","language":[{"iso":"eng"}],"ddc":["570"],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"year":"2025","pmid":1,"quality_controlled":"1","publication_status":"published","intvolume":"        44","date_published":"2025-03-25T00:00:00Z","file_date_updated":"2025-03-17T10:26:54Z","has_accepted_license":"1","article_processing_charge":"Yes","fulldoi":"https://doi.org/10.1016/j.celrep.2025.115387","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"day":"25","corr_author":"1","oa":1,"external_id":{"isi":["001443652700001"],"pmid":["40057955"]},"month":"03","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"publisher":"Cell Press","article_number":"115387","isi":1,"volume":44,"file":[{"success":1,"checksum":"57e05dd1598c807af0afdb32cec039d3","date_created":"2025-03-17T10:26:54Z","access_level":"open_access","creator":"dernst","relation":"main_file","file_id":"19413","content_type":"application/pdf","date_updated":"2025-03-17T10:26:54Z","file_name":"2025_CellReports_Tavano.pdf","file_size":9067797}],"doi":"10.1016/j.celrep.2025.115387"},{"file_date_updated":"2025-06-10T07:24:46Z","date_published":"2025-06-11T00:00:00Z","has_accepted_license":"1","article_processing_charge":"Yes","fulldoi":"https://doi.org/10.1016/j.bpr.2025.100211","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"quality_controlled":"1","year":"2025","publication_status":"published","intvolume":"         5","article_number":"100211","volume":5,"file":[{"relation":"main_file","creator":"dernst","access_level":"open_access","date_created":"2025-06-10T07:24:46Z","checksum":"4018c833f25a3ad3b57e3577fed70334","success":1,"file_size":7238179,"file_name":"2025_BiophysicalReports_Vorlaufer.pdf","date_updated":"2025-06-10T07:24:46Z","content_type":"application/pdf","file_id":"19802"}],"doi":"10.1016/j.bpr.2025.100211","day":"11","corr_author":"1","oa":1,"department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"FlSc"},{"_id":"EM-Fac"}],"month":"06","publisher":"Cell Press","related_material":{"record":[{"status":"public","id":"20206","relation":"dissertation_contains"},{"id":"22744","relation":"dissertation_contains","status":"public"}]},"project":[{"grant_number":"CZI01","_id":"62909c6f-2b32-11ec-9570-e1476aab5308","name":"CryoMinflux-guided in-situ molecular census and structure determination"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","grant_number":"26137"},{"grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020"},{"call_identifier":"FWF","name":"Molecular Drug Targets","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24"},{"name":"High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration","_id":"2668BFA0-B435-11E9-9278-68D0E5697425","grant_number":"LT00057"}],"DOAJ_listed":"1","citation":{"apa":"Vorlaufer, J., Semenov, N., Kreuzinger, C., Javoor, M., Zens, B., Agudelo Duenas, N., … Danzl, J. G. (2025). Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. <i>Biophysical Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">https://doi.org/10.1016/j.bpr.2025.100211</a>","chicago":"Vorlaufer, Jakob, Nikolai Semenov, Caroline Kreuzinger, Manjunath Javoor, Bettina Zens, Nathalie Agudelo Duenas, Mojtaba Tavakoli, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” <i>Biophysical Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">https://doi.org/10.1016/j.bpr.2025.100211</a>.","short":"J. Vorlaufer, N. Semenov, C. Kreuzinger, M. Javoor, B. Zens, N. Agudelo Duenas, M. Tavakoli, M. Suplata, W. Jahr, J. Lyudchik, A. Wartak, F.K. Schur, J.G. Danzl, Biophysical Reports 5 (2025).","ista":"Vorlaufer J, Semenov N, Kreuzinger C, Javoor M, Zens B, Agudelo Duenas N, Tavakoli M, Suplata M, Jahr W, Lyudchik J, Wartak A, Schur FK, Danzl JG. 2025. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. Biophysical Reports. 5(2), 100211.","ieee":"J. Vorlaufer <i>et al.</i>, “Image-based 3D active sample stabilization on the nanometer scale for optical microscopy,” <i>Biophysical Reports</i>, vol. 5, no. 2. Cell Press, 2025.","mla":"Vorlaufer, Jakob, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” <i>Biophysical Reports</i>, vol. 5, no. 2, 100211, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">10.1016/j.bpr.2025.100211</a>.","ama":"Vorlaufer J, Semenov N, Kreuzinger C, et al. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. <i>Biophysical Reports</i>. 2025;5(2). doi:<a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">10.1016/j.bpr.2025.100211</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:55:34Z","_id":"19795","title":"Image-based 3D active sample stabilization on the nanometer scale for optical microscopy","status":"public","date_created":"2025-06-08T22:01:22Z","author":[{"full_name":"Vorlaufer, Jakob","orcid":"0009-0000-7590-3501","first_name":"Jakob","last_name":"Vorlaufer","id":"937696FA-C996-11E9-8C7C-CF13E6697425"},{"full_name":"Semenov, Nikolai","last_name":"Semenov","first_name":"Nikolai","id":"e64d39c7-72ef-11ef-b75a-ee3046860d1b"},{"last_name":"Kreuzinger","first_name":"Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","full_name":"Kreuzinger, Caroline"},{"orcid":"0000-0003-2311-2112","full_name":"Javoor, Manjunath","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","first_name":"Manjunath","last_name":"Javoor"},{"id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina","last_name":"Zens","orcid":"0000-0002-9561-1239","full_name":"Zens, Bettina"},{"full_name":"Agudelo Duenas, Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","last_name":"Agudelo Duenas","first_name":"Nathalie"},{"last_name":"Tavakoli","first_name":"Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba"},{"id":"EE8452B8-C26A-11E9-B157-E80CE6697425","last_name":"Suplata","first_name":"Marek","full_name":"Suplata, Marek"},{"orcid":"0000-0003-0201-2315","full_name":"Jahr, Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","first_name":"Wiebke","last_name":"Jahr"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","last_name":"Lyudchik","full_name":"Lyudchik, Julia"},{"first_name":"Andreas","last_name":"Wartak","id":"60aaa06c-3de5-11eb-9e53-baa88e955dcb","full_name":"Wartak, Andreas"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian Km","last_name":"Schur","full_name":"Schur, Florian Km","orcid":"0000-0003-4790-8078"},{"full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","last_name":"Danzl","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87"}],"article_type":"original","type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Super-resolution microscopy often entails long acquisition times of minutes to hours. Since drifts during the acquisition adversely affect data quality, active sample stabilization is commonly used for some of these techniques to reach their full potential. Although drifts in the lateral plane can often be corrected after acquisition, this is not always possible or may come with drawbacks. Therefore, it is appealing to stabilize sample position in three dimensions (3D) during acquisition. Various schemes for active sample stabilization have been demonstrated previously, with some reaching sub-nanometer stability in 3D. Here, we present a scheme for active drift correction that delivers the nanometer-scale 3D stability demanded by state-of-the-art super-resolution techniques and is straightforward to implement compared to previous schemes capable of reaching this level of stabilization precision. Using a refined algorithm that can handle various types of reference structure, without sparse signal peaks being mandatory, we stabilized sample position to ∼1 nm in 3D using objective lenses both with high and low numerical aperture. Our implementation requires only the addition of a simple widefield imaging path and we provide an open-source control software with graphical user interface to facilitate easy adoption of the module. Finally, we demonstrate how this has the potential to enhance data collection for diffraction-limited and super-resolution imaging techniques using single-molecule localization microscopy and cryo-confocal imaging as showcases.","lang":"eng"}],"OA_place":"publisher","OA_type":"gold","language":[{"iso":"eng"}],"publication":"Biophysical Reports","issue":"2","scopus_import":"1","publication_identifier":{"eissn":["2667-0747"]},"acknowledgement":"We acknowledge expert support by ISTA’s scientific service units, including the Miba Machine Shop, the Electron Microscopy Facility, and the Lab Support Facility. This work has been made possible in part by CZI grant DAF2021-234754 and grant DOI: https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (funder DOI: https://doi.org/10.13039/100014989) (F.K.M.S. and J.G.D.). We further gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant DK W1232 (M.R.T. and J.G.D.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.L.); ISTA postdoctoral fellowship IST fellow (A.W.); and Human Frontier Science Program postdoctoral fellowship LT000557/2018 (W.J.).","ec_funded":1},{"language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","acknowledgement":"We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant collection. We also thank the Social Immunity team, in particular David Moreno Martínez, Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular support, as well as Friedrich Fochler for technical support with the chemical analysis, and the ISTA Lab Support Facility, including the mass spectrometry unit, for general and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull and Michael Sixt for project discussion, and the Social Immunity team for comments on the manuscript. The study was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation Programme (No. 771402; EPIDEMICSonCHIP) to SC.","ec_funded":1,"publication_identifier":{"eissn":["2041-1723"]},"publication":"Nature Communications","scopus_import":"1","date_updated":"2026-10-21T10:53:39Z","supplementarymaterial":"no","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","PlanS_conform":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/ants-signal-deadly-infection/","description":"News on ISTA website","relation":"press_release"}],"record":[{"relation":"research_data","id":"20471","status":"public"}]},"citation":{"short":"E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J. Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S. Cremer, Nature Communications 16 (2025).","ista":"Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic disease signalling in ant colonies. Nature Communications. 16, 10511.","ieee":"E. Dawson <i>et al.</i>, “Altruistic disease signalling in ant colonies,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","ama":"Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>","mla":"Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>, vol. 16, 10511, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>.","apa":"Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L., Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>","chicago":"Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse, Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>."},"DOAJ_listed":"1","project":[{"_id":"2649B4DE-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Epidemics in ant societies on a chip","grant_number":"771402"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"type":"journal_article","oa_version":"Published Version","article_type":"original","abstract":[{"lang":"eng","text":"Sick individuals often conceal their disease status to group members, thereby preventing social exclusion or aggression. Here we show by behavioural, chemical, immunological and infection load analyses that sick ant pupae instead actively emit a chemical signal that in itself is sufficient to trigger their own destruction by colony members. In our experiments, this altruistic disease-signalling was performed only by worker but not queen pupae. The lack of signalling by queen pupae did not constitute cheating behaviour, but reflected their superior immune capabilities. Worker pupae suffered from extensive pathogen replication whereas queen pupae were able to restrain their infection. Our data suggest the evolution of a finely-tuned signalling system in which it is not the induction of an individual’s immune response, but rather its failure to overcome the infection, that triggers pupal signalling for sacrifice. This demonstrates a balanced interplay between individual and social immunity that efficiently achieves whole-colony health."}],"title":"Altruistic disease signalling in ant colonies","_id":"18892","author":[{"full_name":"Dawson, Erika","id":"31B4E2D0-F248-11E8-B48F-1D18A9856A87","first_name":"Erika","last_name":"Dawson"},{"id":"953894f3-25bd-11ec-8556-f70a9d38ef60","first_name":"Michaela","last_name":"Hönigsberger","full_name":"Hönigsberger, Michaela"},{"id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","first_name":"Niklas","last_name":"Kampleitner","full_name":"Kampleitner, Niklas"},{"full_name":"Grasse, Anna V","id":"406F989C-F248-11E8-B48F-1D18A9856A87","last_name":"Grasse","first_name":"Anna V"},{"full_name":"Lindorfer, Lukas","last_name":"Lindorfer","first_name":"Lukas","id":"85f0e6d3-06b3-11ec-8982-8c5049fa4455"},{"full_name":"Robb, Jennifer","last_name":"Robb","first_name":"Jennifer","id":"7bc2734a-e2c6-11ea-9824-a2ed5f0662a8"},{"full_name":"Beikzadeh Abbasi, Farnaz","first_name":"Farnaz","last_name":"Beikzadeh Abbasi","id":"0344bfb9-3feb-11ee-87e9-c27edc800bcd"},{"first_name":"Florian","last_name":"Strahodinsky","id":"979E35EE-C996-11E9-8C7C-CF13E6697425","full_name":"Strahodinsky, Florian"},{"full_name":"Leitner, Hanna","id":"8fc5c6f6-5903-11ec-abad-c83f046253e7","last_name":"Leitner","first_name":"Hanna"},{"id":"876b6b34-8ff4-11ec-97c9-8d95a7aae416","last_name":"Rajendran","first_name":"Harikrishnan","full_name":"Rajendran, Harikrishnan"},{"full_name":"Schmitt, Thomas","last_name":"Schmitt","first_name":"Thomas"},{"id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","first_name":"Sylvia","last_name":"Cremer","orcid":"0000-0002-2193-3868","full_name":"Cremer, Sylvia"}],"status":"public","date_created":"2025-01-27T11:28:05Z","volume":16,"doi":"10.1038/s41467-025-66175-z","researchdata_availability":"no","file":[{"file_size":805323,"file_name":"2025_NatureComm_Dawson.pdf","content_type":"application/pdf","date_updated":"2025-12-15T13:30:33Z","file_id":"20826","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"06244623bb7611c636652ecbc4787889","date_created":"2025-12-15T13:30:33Z","success":1}],"article_number":"10511","external_id":{"pmid":["41330896"]},"publisher":"Springer Nature","month":"12","department":[{"_id":"SyCr"},{"_id":"LifeSc"}],"day":"01","oa":1,"corr_author":"1","das_tickbox":"0","fulldoi":"https://doi.org/10.1038/s41467-025-66175-z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.27.582277"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"}],"APC_amount":"7068 EUR","has_accepted_license":"1","file_date_updated":"2025-12-15T13:30:33Z","date_published":"2025-12-01T00:00:00Z","article_processing_charge":"Yes","publication_status":"published","quality_controlled":"1","pmid":1,"year":"2025","intvolume":"        16","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"]},{"abstract":[{"lang":"eng","text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo."}],"oa_version":"Preprint","type":"preprint","author":[{"full_name":"Dunajova, Zuzana","last_name":"Dunajova","first_name":"Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","last_name":"Tasciyan"},{"id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","first_name":"Juraj","last_name":"Majek","full_name":"Majek, Juraj"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin"},{"full_name":"Sahai, Erik","last_name":"Sahai","first_name":"Erik"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo"}],"status":"public","date_created":"2026-03-11T08:40:06Z","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","_id":"21427","date_updated":"2026-10-22T22:31:26Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>bioRxiv</i>. .","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv, <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, BioRxiv (n.d.)."},"project":[{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793"},{"grant_number":"26360","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","name":"Motile active matter models of migrating cells and chiral filaments"}],"related_material":{"record":[{"status":"public","relation":"research_data","id":"21439"},{"status":"public","relation":"later_version","id":"22963"},{"status":"public","relation":"dissertation_contains","id":"21423"}]},"acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","publication":"bioRxiv","language":[{"iso":"eng"}],"OA_place":"repository","publication_status":"draft","year":"2025","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"ddc":["539","570"],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.20.655037"}],"das_tickbox":"1","fulldoi":"https://doi.org/10.1101/2025.05.20.655037","article_processing_charge":"No","has_accepted_license":"1","date_published":"2025-09-25T00:00:00Z","month":"09","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"corr_author":"1","oa":1,"day":"25","doi":"10.1101/2025.05.20.655037"}]