---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '18892'
abstract:
- lang: eng
text: Sick individuals often conceal their disease status to group members, thereby
preventing social exclusion or aggression. Here we show by behavioural, chemical,
immunological and infection load analyses that sick ant pupae instead actively
emit a chemical signal that in itself is sufficient to trigger their own destruction
by colony members. In our experiments, this altruistic disease-signalling was
performed only by worker but not queen pupae. The lack of signalling by queen
pupae did not constitute cheating behaviour, but reflected their superior immune
capabilities. Worker pupae suffered from extensive pathogen replication whereas
queen pupae were able to restrain their infection. Our data suggest the evolution
of a finely-tuned signalling system in which it is not the induction of an individual’s
immune response, but rather its failure to overcome the infection, that triggers
pupal signalling for sacrifice. This demonstrates a balanced interplay between
individual and social immunity that efficiently achieves whole-colony health.
acknowledged_ssus:
- _id: LifeSc
- _id: MassSpec
acknowledgement: We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal
strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant
collection. We also thank the Social Immunity team, in particular David Moreno Martínez,
Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular
support, as well as Friedrich Fochler for technical support with the chemical analysis,
and the ISTA Lab Support Facility, including the mass spectrometry unit, for general
and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C
calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull
and Michael Sixt for project discussion, and the Social Immunity team for comments
on the manuscript. The study was funded by the European Research Council (ERC) under
the European Union’s Horizon 2020 research and innovation Programme (No. 771402;
EPIDEMICSonCHIP) to SC.
article_number: '10511'
article_processing_charge: Yes
article_type: original
author:
- first_name: Erika
full_name: Dawson, Erika
id: 31B4E2D0-F248-11E8-B48F-1D18A9856A87
last_name: Dawson
- first_name: Michaela
full_name: Hönigsberger, Michaela
id: 953894f3-25bd-11ec-8556-f70a9d38ef60
last_name: Hönigsberger
- first_name: Niklas
full_name: Kampleitner, Niklas
id: 2AC57FAC-F248-11E8-B48F-1D18A9856A87
last_name: Kampleitner
- first_name: Anna V
full_name: Grasse, Anna V
id: 406F989C-F248-11E8-B48F-1D18A9856A87
last_name: Grasse
- first_name: Lukas
full_name: Lindorfer, Lukas
id: 85f0e6d3-06b3-11ec-8982-8c5049fa4455
last_name: Lindorfer
- first_name: Jennifer
full_name: Robb, Jennifer
id: 7bc2734a-e2c6-11ea-9824-a2ed5f0662a8
last_name: Robb
- first_name: Farnaz
full_name: Beikzadeh Abbasi, Farnaz
id: 0344bfb9-3feb-11ee-87e9-c27edc800bcd
last_name: Beikzadeh Abbasi
- first_name: Florian
full_name: Strahodinsky, Florian
id: 979E35EE-C996-11E9-8C7C-CF13E6697425
last_name: Strahodinsky
- first_name: Hanna
full_name: Leitner, Hanna
id: 8fc5c6f6-5903-11ec-abad-c83f046253e7
last_name: Leitner
- first_name: Harikrishnan
full_name: Rajendran, Harikrishnan
id: 876b6b34-8ff4-11ec-97c9-8d95a7aae416
last_name: Rajendran
- first_name: Thomas
full_name: Schmitt, Thomas
last_name: Schmitt
- first_name: Sylvia
full_name: Cremer, Sylvia
id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
last_name: Cremer
orcid: 0000-0002-2193-3868
citation:
ama: Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling
in ant colonies. Nature Communications. 2025;16. doi:10.1038/s41467-025-66175-z
apa: Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L.,
Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies.
Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-025-66175-z
chicago: Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse,
Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling
in Ant Colonies.” Nature Communications. Springer Nature, 2025. https://doi.org/10.1038/s41467-025-66175-z.
ieee: E. Dawson et al., “Altruistic disease signalling in ant colonies,”
Nature Communications, vol. 16. Springer Nature, 2025.
ista: Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh
F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic
disease signalling in ant colonies. Nature Communications. 16, 10511.
mla: Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” Nature
Communications, vol. 16, 10511, Springer Nature, 2025, doi:10.1038/s41467-025-66175-z.
short: E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J.
Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S.
Cremer, Nature Communications 16 (2025).
corr_author: '1'
date_created: 2025-01-27T11:28:05Z
date_published: 2025-12-01T00:00:00Z
date_updated: 2026-04-28T12:57:04Z
day: '01'
ddc:
- '570'
department:
- _id: SyCr
- _id: LifeSc
doi: 10.1038/s41467-025-66175-z
ec_funded: 1
external_id:
pmid:
- '41330896'
file:
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checksum: 06244623bb7611c636652ecbc4787889
content_type: application/pdf
creator: dernst
date_created: 2025-12-15T13:30:33Z
date_updated: 2025-12-15T13:30:33Z
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success: 1
file_date_updated: 2025-12-15T13:30:33Z
has_accepted_license: '1'
intvolume: ' 16'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.02.27.582277
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2649B4DE-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771402'
name: Epidemics in ant societies on a chip
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/ants-signal-deadly-infection/
record:
- id: '20471'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Altruistic disease signalling in ant colonies
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 16
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '17468'
abstract:
- lang: eng
text: Oxygen redox chemistry is central to life1 and many human-made technologies,
such as in energy storage2,3,4. The large energy gain from oxygen redox reactions
is often connected with the occurrence of harmful reactive oxygen species3,5,6.
Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which
may evolve from superoxide. However, the factors determining the formation of
singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown.
Here we report that the release of triplet or singlet oxygen is governed by individual
Marcus normal and inverted region behaviour. We found that as the driving force
for the reaction increases, the initially dominant evolution of triplet oxygen
slows down, and singlet oxygen evolution becomes predominant with higher maximum
kinetics. This behaviour also applies to the widely observed superoxide disproportionation,
in which one superoxide is oxidized by another, in both non-aqueous and aqueous
systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet
oxygen yields governed by these conditions are relevant, for example, in batteries
or cellular organelles in which superoxide forms. Our findings suggest ways to
understand and control spin states and kinetics in oxygen redox chemistry, with
implications for fields, including life sciences, pure chemistry and energy storage.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: M-Shop
- _id: ScienComp
acknowledgement: S.A.F. thanks the Institute of Science and Technology Austria (ISTA)
for the support. The Scientific Service Units of ISTA supported this research through
resources provided by the Imaging and Optics Facility, the Lab Support Facility,
the Miba Machine Shop and Scientific Computing. This research was partly funded
by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open
access purposes, the author has applied for a CC BY public copyright licence to
any author-accepted manuscript version arising from this submission. R.H. acknowledges
funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg
Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989).
H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters
programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life
and R. Jethwa for proofreading. Open access funding was provided by ISTA.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Soumyadip
full_name: Mondal, Soumyadip
id: d25d21ef-dc8d-11ea-abe3-ec4576307f48
last_name: Mondal
- first_name: Huyen T.K.
full_name: Nguyen, Huyen T.K.
last_name: Nguyen
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Stefan Alexander
full_name: Freunberger, Stefan Alexander
id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425
last_name: Freunberger
orcid: 0000-0003-2902-5319
citation:
ama: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control
singlet and triplet oxygen evolving from superoxide. Nature. 2025;646(8085):601–605.
doi:10.1038/s41586-025-09587-7
apa: Mondal, S., Nguyen, H. T. K., Hauschild, R., & Freunberger, S. A. (2025).
Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature.
Springer Nature. https://doi.org/10.1038/s41586-025-09587-7
chicago: Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander
Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from
Superoxide.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-09587-7.
ieee: S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics
control singlet and triplet oxygen evolving from superoxide,” Nature, vol.
646, no. 8085. Springer Nature, pp. 601–605, 2025.
ista: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control
singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605.
mla: Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen
Evolving from Superoxide.” Nature, vol. 646, no. 8085, Springer Nature,
2025, pp. 601–605, doi:10.1038/s41586-025-09587-7.
short: S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025)
601–605.
corr_author: '1'
date_created: 2024-08-29T10:40:23Z
date_published: 2025-10-16T00:00:00Z
date_updated: 2026-04-28T13:18:33Z
day: '16'
ddc:
- '540'
department:
- _id: StFr
- _id: Bio
doi: 10.1038/s41586-025-09587-7
external_id:
isi:
- '001586378900001'
pmid:
- '41044415'
file:
- access_level: open_access
checksum: b507ddd23df0388aa65d04dc9b00fe3d
content_type: application/pdf
creator: dernst
date_created: 2025-10-20T10:26:13Z
date_updated: 2025-10-20T10:26:13Z
file_id: '20500'
file_name: 2025_Nature_Mondal.pdf
file_size: 3809247
relation: main_file
success: 1
file_date_updated: 2025-10-20T10:26:13Z
has_accepted_license: '1'
intvolume: ' 646'
isi: 1
issue: '8085'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 601–605
pmid: 1
project:
- _id: 8df062be-16d5-11f0-9cad-f559b6612c7e
grant_number: P37169
name: Singlet oxygen in non-aqueous oxygen redox chemistry
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/taming-the-bad-oxygen/
scopus_import: '1'
status: public
title: Marcus kinetics control singlet and triplet oxygen evolving from superoxide
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 646
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20082'
abstract:
- lang: eng
text: Efficient immune responses rely on the capacity of leukocytes to traverse
diverse and complex tissues. To meet such changing environmental conditions, leukocytes
usually adopt an ameboid configuration, using their forward-positioned nucleus
as a probe to identify and follow the path of least resistance among pre-existing
pores. We show that, in dense environments where even the largest pores preclude
free passage, leukocytes position their nucleus behind the centrosome and organelles.
The local compression imposed on the cell body by its surroundings triggers assembly
of a central F-actin pool, located between cell front and nucleus. Central actin
pushes outward to transiently dilate a path for organelles and nucleus. Pools
of central and front actin are tightly coupled and experimental depletion of the
central pool enhances actin accumulation and protrusion formation at the cell
front. Although this shifted balance speeds up cells in permissive environments,
migration in restrictive environments is impaired, as the unleashed leading edge
dissociates from the trapped cell body. Our findings establish an actin regulatory
loop that balances path dilation with advancement of the leading edge to maintain
cellular coherence.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This research was supported by the Scientific Service Units of ISTA
through resources provided by the Imaging and Optics, Preclinical and Lab Support
Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus
Service Team, who helped generating the lentiviral particles used in this study.
We thank all the members of the Sixt group for valuable discussions and feedback,
in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing
the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback
during the writing of this manuscript. This work was supported by a European Research
Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral
Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian
Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical
Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB,
Kyushu University. Open access funding provided by Institute of Science and Technology
(IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Patricia
full_name: Dos Reis Rodrigues, Patricia
id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F
last_name: Dos Reis Rodrigues
orcid: 0000-0003-1681-508X
- first_name: Mario
full_name: Avellaneda Sarrió, Mario
id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E
last_name: Avellaneda Sarrió
orcid: 0000-0001-6406-524X
- first_name: Nikola
full_name: Canigova, Nikola
id: 3795523E-F248-11E8-B48F-1D18A9856A87
last_name: Canigova
orcid: 0000-0002-8518-5926
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
- first_name: Kari
full_name: Vaahtomeri, Kari
id: 368EE576-F248-11E8-B48F-1D18A9856A87
last_name: Vaahtomeri
orcid: 0000-0001-7829-3518
- first_name: Michael
full_name: Riedl, Michael
id: 3BE60946-F248-11E8-B48F-1D18A9856A87
last_name: Riedl
orcid: 0000-0003-4844-6311
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Yoshinori
full_name: Fukui, Yoshinori
last_name: Fukui
- first_name: Alba
full_name: Juanes Garcia, Alba
id: 40F05888-F248-11E8-B48F-1D18A9856A87
last_name: Juanes Garcia
orcid: 0000-0002-1009-9652
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune
cells globally coordinate protrusive forces. Nature Immunology. 2025;26:1258–1266.
doi:10.1038/s41590-025-02211-w
apa: Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R.,
Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally
coordinate protrusive forces. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-025-02211-w
chicago: Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova,
Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating
Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology.
Springer Nature, 2025. https://doi.org/10.1038/s41590-025-02211-w.
ieee: P. Dos Reis Rodrigues et al., “Migrating immune cells globally coordinate
protrusive forces,” Nature Immunology, vol. 26. Springer Nature, pp. 1258–1266,
2025.
ista: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri
K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt
MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature
Immunology. 26, 1258–1266.
mla: Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate
Protrusive Forces.” Nature Immunology, vol. 26, Springer Nature, 2025,
pp. 1258–1266, doi:10.1038/s41590-025-02211-w.
short: P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K.
Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes
Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.
corr_author: '1'
date_created: 2025-07-27T22:01:26Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-04-28T13:26:50Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/s41590-025-02211-w
external_id:
isi:
- '001529134300001'
pmid:
- '40664976'
file:
- access_level: open_access
checksum: 0c725123dca7797c682609bff2c4c5ac
content_type: application/pdf
creator: dernst
date_created: 2025-07-31T08:00:33Z
date_updated: 2025-07-31T08:00:33Z
file_id: '20096'
file_name: 2025_NatureImmunology_ReisRodrigues.pdf
file_size: 13514646
relation: main_file
success: 1
file_date_updated: 2025-07-31T08:00:33Z
has_accepted_license: '1'
intvolume: ' 26'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 1258–1266
pmid: 1
project:
- _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd
grant_number: '101071793'
name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal
pushing forces'
- _id: c092d618-5a5b-11eb-8a69-f92e1e843fc8
grant_number: 944-2020
name: 'Bioelectric patrolling: the role of the local membrane potential in immune
cell migration'
publication: Nature Immunology
publication_identifier:
eissn:
- 1529-2916
issn:
- 1529-2908
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/bench-pressing-cells/
record:
- id: '20149'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Migrating immune cells globally coordinate protrusive forces
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 26
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19704'
abstract:
- lang: eng
text: The information-processing capability of the brain’s cellular network depends
on the physical wiring pattern between neurons and their molecular and functional
characteristics. Mapping neurons and resolving their individual synaptic connections
can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular
labelling. Light microscopy is uniquely positioned to visualize specific molecules,
but dense, synapse-level circuit reconstruction by light microscopy has been out
of reach, owing to limitations in resolution, contrast and volumetric imaging
capability. Here we describe light-microscopy-based connectomics (LICONN). We
integrated specifically engineered hydrogel embedding and expansion with comprehensive
deep-learning-based segmentation and analysis of connectivity, thereby directly
incorporating molecular information into synapse-level reconstructions of brain
tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological
experiments in a readily adoptable manner.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
- _id: PreCl
- _id: M-Shop
- _id: E-Lib
acknowledgement: 'We thank S. Dorkenwald and P. Li for critical reading of the manuscript,
S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge
support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific
Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge
funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D.
and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft
für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s
Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions
Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation
programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open
access funding provided by Institute of Science and Technology (IST Austria).'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mojtaba
full_name: Tavakoli, Mojtaba
id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87
last_name: Tavakoli
orcid: 0000-0002-7667-6854
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Michał
full_name: Januszewski, Michał
last_name: Januszewski
- first_name: Vitali
full_name: Vistunou, Vitali
id: 7e146587-8972-11ed-ae7b-d7a32ea86a81
last_name: Vistunou
- first_name: Nathalie
full_name: Agudelo Duenas, Nathalie
id: 40E7F008-F248-11E8-B48F-1D18A9856A87
last_name: Agudelo Duenas
- first_name: Jakob
full_name: Vorlaufer, Jakob
id: 937696FA-C996-11E9-8C7C-CF13E6697425
last_name: Vorlaufer
orcid: 0009-0000-7590-3501
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Bárbara
full_name: Oliveira, Bárbara
id: 3B03AA1A-F248-11E8-B48F-1D18A9856A87
last_name: Oliveira
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Viren
full_name: Jain, Viren
last_name: Jain
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic
reconstruction of mammalian brain tissue. Nature. 2025;642:398-410. doi:10.1038/s41586-025-08985-1
apa: Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas,
N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction
of mammalian brain tissue. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08985-1
chicago: Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou,
Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based
Connectomic Reconstruction of Mammalian Brain Tissue.” Nature. Springer
Nature, 2025. https://doi.org/10.1038/s41586-025-08985-1.
ieee: M. Tavakoli et al., “Light-microscopy-based connectomic reconstruction
of mammalian brain tissue,” Nature, vol. 642. Springer Nature, pp. 398–410,
2025.
ista: Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer
J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl
JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain
tissue. Nature. 642, 398–410.
mla: Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction
of Mammalian Brain Tissue.” Nature, vol. 642, Springer Nature, 2025, pp.
398–410, doi:10.1038/s41586-025-08985-1.
short: M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas,
J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino,
V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.
corr_author: '1'
date_created: 2025-05-18T22:02:51Z
date_published: 2025-06-12T00:00:00Z
date_updated: 2026-04-28T13:33:34Z
day: '12'
ddc:
- '570'
department:
- _id: JoDa
- _id: GradSch
- _id: Bio
- _id: GaNo
doi: 10.1038/s41586-025-08985-1
ec_funded: 1
external_id:
isi:
- '001483477000001'
pmid:
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file:
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creator: dernst
date_created: 2025-07-03T06:55:20Z
date_updated: 2025-07-03T06:55:20Z
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file_name: 2025_Nature_Tavakoli.pdf
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language:
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month: '06'
oa: 1
oa_version: Published Version
page: 398-410
pmid: 1
project:
- _id: 6285a163-2b32-11ec-9570-8e204ca2dba5
grant_number: '26137'
name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion
Microscopy
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6
grant_number: '101044865'
name: Toward an understanding of the brain interstitial system and the extracellular
proteome in health and autism spectrum disorders
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
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publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
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relation: press_release
url: https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/
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scopus_import: '1'
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title: Light-microscopy-based connectomic reconstruction of mammalian brain tissue
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
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year: '2025'
...
---
OA_place: publisher
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abstract:
- lang: eng
text: Active regulation of gene expression, orchestrated by complex interactions
of activators and repressors at promoters, controls the fate of organisms. In
contrast, basal expression at uninduced promoters is considered to be a dynamically
inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional
regulation. Here, we investigate the basal expression mode of the mar operon,
the main regulator of intrinsic multiple antibiotic resistance in Escherichia
coli, and link its dynamic properties to the noncanonical, yet highly conserved
start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements
across tens of generations reveal that basal expression consists of rare stochastic
gene expression pulses, which maximize variability in wildtype and, surprisingly,
transiently accelerate cellular elongation rates. Competition experiments show
that basal expression confers fitness advantages to wildtype across several transitions
between exponential and stationary growth by shortening lag times. The dynamically
rich basal expression of the mar operon has likely been evolutionarily maintained
for its role in growth homeostasis of Enterobacteria within the gut environment,
thereby allowing other ancillary gene regulatory roles to evolve, e.g., control
of costly-to-induce multidrug efflux pumps. Understanding the complex selection
forces governing genetic systems involved in intrinsic multidrug resistance is
crucial for effective public health measures.
acknowledged_ssus:
- _id: Bio
acknowledgement: K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions
on the manuscript, all other members from the Guet laboratory for valuable feedback,
R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria
for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic
Technology India for allowing time off to address the revisions. K.J. acknowledges
Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported
in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi),
O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant
ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.
article_number: e2413709122
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Kirti
full_name: Jain, Kirti
id: 330F0278-F248-11E8-B48F-1D18A9856A87
last_name: Jain
orcid: 0000-0002-3809-0449
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Roderich
full_name: Römhild, Roderich
id: 68E56E44-62B0-11EA-B963-444F3DDC885E
last_name: Römhild
orcid: 0000-0001-9480-5261
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile
basal gene expression as a fitness determinant in bacteria. Proceedings of
the National Academy of Sciences. 2025;122(15). doi:10.1073/pnas.2413709122
apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet,
C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria.
Proceedings of the National Academy of Sciences. National Academy of Sciences.
https://doi.org/10.1073/pnas.2413709122
chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper
Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant
in Bacteria.” Proceedings of the National Academy of Sciences. National
Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2413709122.
ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet,
“Pulsatile basal gene expression as a fitness determinant in bacteria,” Proceedings
of the National Academy of Sciences, vol. 122, no. 15. National Academy of
Sciences, 2025.
ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile
basal gene expression as a fitness determinant in bacteria. Proceedings of the
National Academy of Sciences. 122(15), e2413709122.
mla: Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant
in Bacteria.” Proceedings of the National Academy of Sciences, vol. 122,
no. 15, e2413709122, National Academy of Sciences, 2025, doi:10.1073/pnas.2413709122.
short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings
of the National Academy of Sciences 122 (2025).
corr_author: '1'
date_created: 2025-04-27T22:02:13Z
date_published: 2025-04-15T00:00:00Z
date_updated: 2026-04-28T13:35:46Z
day: '15'
ddc:
- '570'
department:
- _id: CaGu
- _id: Bio
- _id: FyKo
- _id: GaTk
doi: 10.1073/pnas.2413709122
external_id:
isi:
- '001471235200001'
pmid:
- '40193613'
file:
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pmid: 1
project:
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publication: Proceedings of the National Academy of Sciences
publication_identifier:
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issn:
- 0027-8424
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publisher: National Academy of Sciences
quality_controlled: '1'
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title: Pulsatile basal gene expression as a fitness determinant in bacteria
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
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short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 122
year: '2025'
...
---
OA_place: repository
OA_type: gold
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abstract:
- lang: eng
text: Active regulation of gene expression, orchestrated by complex interactions
of activators and repressors at promoters, controls the fate of organisms. In
contrast, basal expression at uninduced promoters is considered to be a dynamically
inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional
regulation. Here, we investigate the basal expression mode of the mar operon,
the main regulator of intrinsic multiple antibiotic resistance in Escherichia
coli, and link its dynamic properties to the non-canonical, yet highly conserved
start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements
across tens of generations reveal that basal expression consists of rare stochastic
gene expression pulses, which maximize variability in wildtype and, surprisingly,
transiently accelerate cellular elongation rates. Competition experiments show
that basal expression confers fitness advantages to wildtype across several transitions
between exponential and stationary growth by shortening lag times. The dynamically
rich basal expression of the mar operon has likely been evolutionarily maintained
for its role in growth homeostasis of Enterobacteria within the gut environment,
thereby allowing other ancillary gene regulatory roles to evolve, e.g. control
of costly-to-induce multi-drug efflux pumps. Understanding the complex selection
forces governing genetic systems involved in intrinsic multi-drug resistance is
crucial for effective public health measures.
article_processing_charge: No
author:
- first_name: Kirti
full_name: Jain, Kirti
id: 330F0278-F248-11E8-B48F-1D18A9856A87
last_name: Jain
orcid: 0000-0002-3809-0449
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Roderich
full_name: Römhild, Roderich
id: 68E56E44-62B0-11EA-B963-444F3DDC885E
last_name: Römhild
orcid: 0000-0001-9480-5261
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile
basal gene expression as a fitness determinant in bacteria.” 2025. doi:10.15479/AT:ISTA:19294
apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet,
C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant
in bacteria.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:19294
chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper
Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025.
https://doi.org/10.15479/AT:ISTA:19294.
ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet,
“Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’”
Institute of Science and Technology Austria, 2025.
ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data
for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:19294.
mla: Jain, Kirti, et al. Data for “Pulsatile Basal Gene Expression as a Fitness
Determinant in Bacteria.” Institute of Science and Technology Austria, 2025,
doi:10.15479/AT:ISTA:19294.
short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).
corr_author: '1'
date_created: 2025-03-04T13:27:21Z
date_published: 2025-03-04T00:00:00Z
date_updated: 2026-04-28T13:35:45Z
day: '04'
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- _id: Bio
- _id: FyKo
- _id: GaTk
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title: Data for "Pulsatile basal gene expression as a fitness determinant in bacteria"
tmp:
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legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
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...
---
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abstract:
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text: 'When two insulating, neutral materials are contacted and separated, they
exchange electrical charge1. Experiments have long suggested that this ‘contact
electrification’ is transitive, with different materials ordering into ‘triboelectric
series’ based on the sign of charge acquired2. At the same time, the effect is
plagued by unpredictability, preventing consensus on the mechanism and casting
doubt on the rhyme and reason that series imply3. Here we expose an unanticipated
connection between the unpredictability and order in contact electrification:
nominally identical materials initially exchange charge randomly and intransitively,
but—over repeated experiments—order into triboelectric series. We find that this
evolution is driven by the act of contact itself—samples with more contacts in
their history charge negatively to ones with fewer contacts. Capturing this ‘contact
bias’ in a minimal model, we recreate both the initial randomness and ultimate
order in numerical simulations and use it experimentally to force the appearance
of a triboelectric series of our choosing. With a set of surface-sensitive techniques
to search for the underlying alterations contact creates, we only find evidence
of nanoscale morphological changes, pointing to a mechanism strongly coupled with
mechanics. Our results highlight the centrality of contact history in contact
electrification and suggest that focusing on the unpredictability that has long
plagued the effect may hold the key to understanding it.'
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
- _id: EM-Fac
- _id: LifeSc
acknowledgement: This project has received financing from the European Research Council
grant agreement no. 949120 under the European Union’s Horizon 2020 research and
innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges
support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge
the state of Lower Austria and the European Regional Development Fund under grant
no. WST3-F-542638/004-2021. This research was supported by the Scientific Service
Units of the Institute of Science and Technology Austria through resources provided
by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility,
Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez
and G. Anciaux for the suggestion to look into the roughness power spectral density.
We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements.
Open access funding provided by Institute of Science and Technology (IST Austria).
article_number: 664-669
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Juan Carlos A
full_name: Sobarzo Ponce, Juan Carlos A
id: 4B807D68-AE37-11E9-AC72-31CAE5697425
last_name: Sobarzo Ponce
- first_name: Felix
full_name: Pertl, Felix
id: 6313aec0-15b2-11ec-abd3-ed67d16139af
last_name: Pertl
orcid: 0000-0003-0463-5794
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Tommaso
full_name: Costanzo, Tommaso
id: D93824F4-D9BA-11E9-BB12-F207E6697425
last_name: Costanzo
orcid: 0000-0001-9732-3815
- first_name: Markus
full_name: Sauer, Markus
last_name: Sauer
- first_name: Annette
full_name: Foelske, Annette
last_name: Foelske
- first_name: Markus
full_name: Ostermann, Markus
last_name: Ostermann
- first_name: Christian M.
full_name: Pichler, Christian M.
last_name: Pichler
- first_name: Yongkang
full_name: Wang, Yongkang
last_name: Wang
- first_name: Yuki
full_name: Nagata, Yuki
last_name: Nagata
- first_name: Mischa
full_name: Bonn, Mischa
last_name: Bonn
- first_name: Scott R
full_name: Waitukaitis, Scott R
id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
last_name: Waitukaitis
orcid: 0000-0002-2299-3176
citation:
ama: Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical
materials into a triboelectric series. Nature. 2025;638(8051). doi:10.1038/s41586-024-08530-6
apa: Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske,
A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into
a triboelectric series. Nature. Springer Nature. https://doi.org/10.1038/s41586-024-08530-6
chicago: Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo,
Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering
of Identical Materials into a Triboelectric Series.” Nature. Springer Nature,
2025. https://doi.org/10.1038/s41586-024-08530-6.
ieee: J. C. A. Sobarzo Ponce et al., “Spontaneous ordering of identical materials
into a triboelectric series,” Nature, vol. 638, no. 8051. Springer Nature,
2025.
ista: Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann
M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering
of identical materials into a triboelectric series. Nature. 638(8051), 664–669.
mla: Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials
into a Triboelectric Series.” Nature, vol. 638, no. 8051, 664–669, Springer
Nature, 2025, doi:10.1038/s41586-024-08530-6.
short: J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske,
M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature
638 (2025).
corr_author: '1'
date_created: 2025-03-02T23:01:52Z
date_published: 2025-02-20T00:00:00Z
date_updated: 2026-04-28T13:44:56Z
day: '20'
ddc:
- '530'
department:
- _id: ScWa
- _id: LifeSc
- _id: EM-Fac
doi: 10.1038/s41586-024-08530-6
ec_funded: 1
external_id:
isi:
- '001428076100015'
pmid:
- '39972227'
file:
- access_level: open_access
checksum: fecf302274dd3218d3e7dd22f39a6c0c
content_type: application/pdf
creator: dernst
date_created: 2025-03-04T10:05:18Z
date_updated: 2025-03-04T10:05:18Z
file_id: '19289'
file_name: 2025_Nature_Sobarzo.pdf
file_size: 3807415
relation: main_file
success: 1
file_date_updated: 2025-03-04T10:05:18Z
has_accepted_license: '1'
intvolume: ' 638'
isi: 1
issue: '8051'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa
call_identifier: H2020
grant_number: '949120'
name: 'Tribocharge: a multi-scale approach to an enduring problem in physics'
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/
record:
- id: '20203'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Spontaneous ordering of identical materials into a triboelectric series
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 638
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18807'
abstract:
- lang: eng
text: Developing tissues interpret dynamic changes in morphogen activity to generate
cell type diversity. To quantitatively study bone morphogenetic protein (BMP)
signaling dynamics in the mouse neural tube, we developed an embryonic stem cell
differentiation system tailored for growing tissues. Differentiating cells form
striking self-organized patterns of dorsal neural tube cell types driven by sequential
phases of BMP signaling that are observed both in vitro and in vivo. Data-driven
biophysical modeling showed that these dynamics result from coupling fast negative
feedback with slow positive regulation of signaling by the specification of an
endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling
in space, we identify a BMP signaling relay that operates in time. This mechanism
allows for a rapid initial concentration-sensitive response that is robustly terminated,
thereby regulating balanced sequential cell type generation. Our study provides
an experimental and theoretical framework to understand how signaling dynamics
are exploited in developing tissues.
acknowledgement: We thank A. Miller and N. Papalopulu for reagents and J. Briscoe
for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European
Research Council under Horizon Europe, grant 101044579; and the Austrian Science
Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft
für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was
supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral
Fellowship (ALTF 343-2022).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Stefanie
full_name: Rus, Stefanie
id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
last_name: Rus
orcid: 0000-0001-8703-1093
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Thomas
full_name: Minchington, Thomas
id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
last_name: Minchington
- first_name: Martina
full_name: Greunz, Martina
id: 48A59534-F248-11E8-B48F-1D18A9856A87
last_name: Greunz
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Anna
full_name: Kicheva, Anna
id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
last_name: Kicheva
orcid: 0000-0003-4509-4998
citation:
ama: Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in
the developing mouse neural tube by a temporal relay of BMP signaling. Developmental
Cell. 2025;60(4):567-580. doi:10.1016/j.devcel.2024.10.024
apa: Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E.
B., & Kicheva, A. (2025). Self-organized pattern formation in the developing
mouse neural tube by a temporal relay of BMP signaling. Developmental Cell.
Elsevier. https://doi.org/10.1016/j.devcel.2024.10.024
chicago: Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack
Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation
in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” Developmental
Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.024.
ieee: S. Rus et al., “Self-organized pattern formation in the developing
mouse neural tube by a temporal relay of BMP signaling,” Developmental Cell,
vol. 60, no. 4. Elsevier, pp. 567–580, 2025.
ista: Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva
A. 2025. Self-organized pattern formation in the developing mouse neural tube
by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.
mla: Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse
Neural Tube by a Temporal Relay of BMP Signaling.” Developmental Cell,
vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:10.1016/j.devcel.2024.10.024.
short: S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo,
A. Kicheva, Developmental Cell 60 (2025) 567–580.
corr_author: '1'
date_created: 2025-01-09T11:25:47Z
date_published: 2025-02-24T00:00:00Z
date_updated: 2026-04-28T22:30:58Z
day: '24'
ddc:
- '570'
department:
- _id: AnKi
- _id: EdHa
- _id: NanoFab
doi: 10.1016/j.devcel.2024.10.024
external_id:
isi:
- '001434279000001'
pmid:
- '39603235'
file:
- access_level: open_access
checksum: bb58db4a908a1f4aabe4004706154541
content_type: application/pdf
creator: dernst
date_created: 2025-04-16T10:54:07Z
date_updated: 2025-04-16T10:54:07Z
file_id: '19584'
file_name: 2025_DevelopmentalCell_Lehr.pdf
file_size: 6994499
relation: main_file
success: 1
file_date_updated: 2025-04-16T10:54:07Z
has_accepted_license: '1'
intvolume: ' 60'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 567-580
pmid: 1
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
grant_number: '101044579'
name: Mechanisms of tissue size regulation in spinal cord development
- _id: 059DF620-7A3F-11EA-A408-12923DDC885E
grant_number: F7802
name: Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen
control of growth and pattern in the spinal cord
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
grant_number: SC19-011
name: The regulatory logic of pattern formation in the vertebrate dorsal neural
tube
publication: Developmental Cell
publication_identifier:
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '19763'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Self-organized pattern formation in the developing mouse neural tube by a temporal
relay of BMP signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 60
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17885'
abstract:
- lang: eng
text: 'The formation of new ribosomes is tightly coordinated with cell growth and
proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and
RNAs follows an intricate scheme of maturation and rearrangement steps across
three cellular compartments: the nucleolus, nucleoplasm, and cytoplasm. We demonstrate
that usnic acid, a lichen secondary metabolite, inhibits the maturation of the
large ribosomal subunit in yeast. We combine biochemical characterization of pre-ribosomal
particles with a quantitative single-particle cryo-EM approach to monitor changes
in nucleolar particle populations upon drug treatment. Usnic acid rapidly blocks
the transition from nucleolar state B to C of Nsa1-associated pre-ribosomes, depleting
key maturation factors such as Dbp10 and hindering pre-rRNA processing. This primary
nucleolar block rapidly rebounds on earlier stages of the pathway which highlights
the regulatory linkages between different steps. In summary, we provide an in-depth
characterization of the effect of usnic acid on ribosome biogenesis, which may
have implications for its reported anti-cancer activities.'
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: We thank Michael A. McAlear, Micheline Fromont-Racin, Philipp Milkereit,
Arlen W. Johnson, Sabine Rospert, Ed Hurt, C. Yam, Günter Daum, Wolfgang Zachariae,
Katrin Karbstein, Juan P. G. Ballesta, Mercedes Dosil, Miguel Remacha und Jesus
de la Cruz for sharing strains or providing antibodies. We thank the members of
the Bergler lab and the Haselbach lab for their helpful discussion. We thank Ellen
Zhong for helpful discussions about the quantitative cryoDRGN analysis. This research
was supported by the Scientific Service Units of IST Austria through resources provided
by the Electron Microscopy Facility. This research was funded in whole, or in part,
by the Austrian Science Foundation grants [https://doi.org/10.55776/P32977], [https://doi.org/10.55776/P29451]
and [https://doi.org/10.55776/P32536] (to H.B.). Research at the IMP is generously
supported by Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter
grant FFG-852936). For the purpose of open access, the author has applied a CC BY
public copyright licence to any Author Accepted Manuscript version arising from
this submission.
article_number: '7511'
article_processing_charge: Yes
article_type: original
author:
- first_name: Lisa
full_name: Kofler, Lisa
last_name: Kofler
- first_name: Lorenz
full_name: Grundmann, Lorenz
last_name: Grundmann
- first_name: Magdalena
full_name: Gerhalter, Magdalena
last_name: Gerhalter
- first_name: Michael
full_name: Prattes, Michael
last_name: Prattes
- first_name: Juliane
full_name: Merl-Pham, Juliane
last_name: Merl-Pham
- first_name: Gertrude
full_name: Zisser, Gertrude
last_name: Zisser
- first_name: Irina
full_name: Grishkovskaya, Irina
last_name: Grishkovskaya
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
orcid: 0000-0003-3904-947X
- first_name: Martin
full_name: Vareka, Martin
last_name: Vareka
- first_name: Rolf
full_name: Breinbauer, Rolf
last_name: Breinbauer
- first_name: Stefanie M.
full_name: Hauck, Stefanie M.
last_name: Hauck
- first_name: David
full_name: Haselbach, David
last_name: Haselbach
- first_name: Helmut
full_name: Bergler, Helmut
last_name: Bergler
citation:
ama: Kofler L, Grundmann L, Gerhalter M, et al. The novel ribosome biogenesis inhibitor
usnic acid blocks nucleolar pre-60S maturation. Nature Communications.
2024;15. doi:10.1038/s41467-024-51754-3
apa: Kofler, L., Grundmann, L., Gerhalter, M., Prattes, M., Merl-Pham, J., Zisser,
G., … Bergler, H. (2024). The novel ribosome biogenesis inhibitor usnic acid blocks
nucleolar pre-60S maturation. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-51754-3
chicago: Kofler, Lisa, Lorenz Grundmann, Magdalena Gerhalter, Michael Prattes, Juliane
Merl-Pham, Gertrude Zisser, Irina Grishkovskaya, et al. “The Novel Ribosome Biogenesis
Inhibitor Usnic Acid Blocks Nucleolar Pre-60S Maturation.” Nature Communications.
Springer Nature, 2024. https://doi.org/10.1038/s41467-024-51754-3.
ieee: L. Kofler et al., “The novel ribosome biogenesis inhibitor usnic acid
blocks nucleolar pre-60S maturation,” Nature Communications, vol. 15. Springer
Nature, 2024.
ista: Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya
I, Hodirnau V-V, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. 2024.
The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.
Nature Communications. 15, 7511.
mla: Kofler, Lisa, et al. “The Novel Ribosome Biogenesis Inhibitor Usnic Acid Blocks
Nucleolar Pre-60S Maturation.” Nature Communications, vol. 15, 7511, Springer
Nature, 2024, doi:10.1038/s41467-024-51754-3.
short: L. Kofler, L. Grundmann, M. Gerhalter, M. Prattes, J. Merl-Pham, G. Zisser,
I. Grishkovskaya, V.-V. Hodirnau, M. Vareka, R. Breinbauer, S.M. Hauck, D. Haselbach,
H. Bergler, Nature Communications 15 (2024).
date_created: 2024-09-08T22:01:10Z
date_published: 2024-08-29T00:00:00Z
date_updated: 2025-09-08T09:13:01Z
day: '29'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.1038/s41467-024-51754-3
external_id:
isi:
- '001457895200001'
pmid:
- '39209816'
file:
- access_level: open_access
checksum: 7c044538a47182c826d1b526c52958a2
content_type: application/pdf
creator: dernst
date_created: 2024-09-09T08:56:12Z
date_updated: 2024-09-09T08:56:12Z
file_id: '17946'
file_name: 2024_NatureComm_Kofler.pdf
file_size: 3735024
relation: main_file
success: 1
file_date_updated: 2024-09-09T08:56:12Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S
maturation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
_id: '18052'
abstract:
- lang: eng
text: Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL)
is an electron microscope (EM) sample preparation technique which allows for high-resolution
visualization of membrane proteins with high sensitivity. However, image acquisition
of specific replica profiles such as synapses in a large field of EM view needs
a valid experience and a long time for manual searching. Here, we describe how
to utilize deep learning for automatizing image acquisition of specific profiles
of interest in replica samples. This protocol facilitates the labor-intensive
collection of EM images, in particular for rare profiles. We provide instructions
for using SerialEM image acquisition software in conjunction with object detection
by our newly developed deep learning software DarEM, to automatically acquire
tilt series of all synapses in a selected region. We then show how to perform
a mostly automated analysis of gold particle labeling in the acquired images by
utilizing Darea software.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: This research was supported by the European Research Council Advanced
Grant 694539 to RS and by the Scientific Service Units of IST Austria through resources
provided by the Electron Microscopy Facility.
alternative_title:
- Neuromethods
article_processing_charge: No
author:
- first_name: David
full_name: Kleindienst, David
id: 42E121A4-F248-11E8-B48F-1D18A9856A87
last_name: Kleindienst
- first_name: Tommaso
full_name: Costanzo, Tommaso
id: D93824F4-D9BA-11E9-BB12-F207E6697425
last_name: Costanzo
orcid: 0000-0001-9732-3815
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: 'Kleindienst D, Costanzo T, Shigemoto R. Automated Imaging and Analysis of
Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: Lübke JHR,
Rollenhagen A, eds. New Aspects in Analyzing the Synaptic Organization of the
Brain. 1st ed. New York: Springer Nature; 2024:123-137. doi:10.1007/978-1-0716-4019-7_8'
apa: 'Kleindienst, D., Costanzo, T., & Shigemoto, R. (2024). Automated Imaging
and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.
In J. H. R. Lübke & A. Rollenhagen (Eds.), New Aspects in Analyzing the
Synaptic Organization of the Brain (1st ed., pp. 123–137). New York: Springer
Nature. https://doi.org/10.1007/978-1-0716-4019-7_8'
chicago: 'Kleindienst, David, Tommaso Costanzo, and Ryuichi Shigemoto. “Automated
Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep
Learning.” In New Aspects in Analyzing the Synaptic Organization of the Brain,
edited by Joachim H.R. Lübke and Astrid Rollenhagen, 1st ed., 123–37. New York:
Springer Nature, 2024. https://doi.org/10.1007/978-1-0716-4019-7_8.'
ieee: 'D. Kleindienst, T. Costanzo, and R. Shigemoto, “Automated Imaging and Analysis
of Synapses in Freeze-Fracture Replica Samples with Deep Learning,” in New
Aspects in Analyzing the Synaptic Organization of the Brain, 1st ed., J. H.
R. Lübke and A. Rollenhagen, Eds. New York: Springer Nature, 2024, pp. 123–137.'
ista: 'Kleindienst D, Costanzo T, Shigemoto R. 2024.Automated Imaging and Analysis
of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: New Aspects
in Analyzing the Synaptic Organization of the Brain. Neuromethods, , 123–137.'
mla: Kleindienst, David, et al. “Automated Imaging and Analysis of Synapses in Freeze-Fracture
Replica Samples with Deep Learning.” New Aspects in Analyzing the Synaptic
Organization of the Brain, edited by Joachim H.R. Lübke and Astrid Rollenhagen,
1st ed., Springer Nature, 2024, pp. 123–37, doi:10.1007/978-1-0716-4019-7_8.
short: D. Kleindienst, T. Costanzo, R. Shigemoto, in:, J.H.R. Lübke, A. Rollenhagen
(Eds.), New Aspects in Analyzing the Synaptic Organization of the Brain, 1st ed.,
Springer Nature, New York, 2024, pp. 123–137.
corr_author: '1'
date_created: 2024-09-10T12:32:38Z
date_published: 2024-08-27T00:00:00Z
date_updated: 2025-04-14T07:27:15Z
day: '27'
department:
- _id: EM-Fac
- _id: RySh
doi: 10.1007/978-1-0716-4019-7_8
ec_funded: 1
edition: '1'
editor:
- first_name: 'Joachim H.R. '
full_name: 'Lübke, Joachim H.R. '
last_name: Lübke
- first_name: Astrid
full_name: Rollenhagen, Astrid
last_name: Rollenhagen
language:
- iso: eng
month: '08'
oa_version: None
page: 123-137
place: New York
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
publication: New Aspects in Analyzing the Synaptic Organization of the Brain
publication_identifier:
eisbn:
- '9781071640197'
eissn:
- 1940-6045
isbn:
- '9781071640180'
issn:
- 0893-2336
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples
with Deep Learning
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '18168'
abstract:
- lang: eng
text: 'Despite the considerable interest in the recombinant production of synthetic
spider silk fibers that possess mechanical properties similar to those of native
spider silks, such as the cost-effectiveness, tunability, and scalability realization,
is still lacking. To address this long-standing challenge, we have constructed
an artificial spider silk gene using Golden Gate assembly for the recombinant
bacterial production of dragline-mimicking silk, incorporating all the essential
components: the N-terminal domain, a 33-residue-long major-ampullate-spidroin-inspired
segment repeated 16 times, and the C-terminal domain (N16C). This designed silk-like
protein was successfully expressed in Escherichia coli, purified, and cast into
films from formic acid. We produced uniformly 13C–15N-labeled N16C films and employed
solid-state magic-angle spinning nuclear magnetic resonance (NMR) for characterization.
Thus, we could demonstrate that our bioengineered silk-like protein self-assembles
into a film where, when hydrated, the solvent-exposed layer of the rigid, β-nanocrystalline
polyalanine core undergoes a transition to an α-helical structure, gaining mobility
to the extent that it fully dissolves in water and transforms into a highly dynamic
random coil. This hydration-induced behavior induces chain dynamics in the glycine-rich
amorphous soft segments on the microsecond time scale, contributing to the elasticity
of the solid material. Our findings not only reveal the presence of structurally
and dynamically distinct segments within the film’s superstructure but also highlight
the complexity of the self-organization responsible for the exceptional mechanical
properties observed in proteins that mimic dragline silk.'
acknowledgement: We thank Dr. Pavel Kielkowski for performing the MS/MS measurement
and providing feedback on the manuscript. We are grateful to Rodrigo Ledesma Amaro
for introducing the Golden Gate Assembly technique in our lab. We acknowledge the
support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)─SFB
1309-325871075, the Center for NanoScience (CeNS), the Fonds der Chemischen Industrie,
and Universitätsgesellschaft München.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Dongqing
full_name: Wu, Dongqing
last_name: Wu
- first_name: Anamaria
full_name: Koscic, Anamaria
last_name: Koscic
- first_name: Sonja
full_name: Schneider, Sonja
last_name: Schneider
- first_name: Romeo C. A.
full_name: Dubini, Romeo C. A.
last_name: Dubini
- first_name: Diana C.
full_name: Rodriguez Camargo, Diana C.
last_name: Rodriguez Camargo
- first_name: Sabine
full_name: Schneider, Sabine
last_name: Schneider
- first_name: Petra
full_name: Rovo, Petra
id: c316e53f-b965-11eb-b128-bb26acc59c00
last_name: Rovo
orcid: 0000-0001-8729-7326
citation:
ama: Wu D, Koscic A, Schneider S, et al. Unveiling the dynamic self-assembly of
a recombinant dragline-silk-mimicking protein. Biomacromolecules. 2024;25(3):1759-1774.
doi:10.1021/acs.biomac.3c01239
apa: Wu, D., Koscic, A., Schneider, S., Dubini, R. C. A., Rodriguez Camargo, D.
C., Schneider, S., & Rovo, P. (2024). Unveiling the dynamic self-assembly
of a recombinant dragline-silk-mimicking protein. Biomacromolecules. American
Chemical Society. https://doi.org/10.1021/acs.biomac.3c01239
chicago: Wu, Dongqing, Anamaria Koscic, Sonja Schneider, Romeo C. A. Dubini, Diana
C. Rodriguez Camargo, Sabine Schneider, and Petra Rovo. “Unveiling the Dynamic
Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.” Biomacromolecules.
American Chemical Society, 2024. https://doi.org/10.1021/acs.biomac.3c01239.
ieee: D. Wu et al., “Unveiling the dynamic self-assembly of a recombinant
dragline-silk-mimicking protein,” Biomacromolecules, vol. 25, no. 3. American
Chemical Society, pp. 1759–1774, 2024.
ista: Wu D, Koscic A, Schneider S, Dubini RCA, Rodriguez Camargo DC, Schneider S,
Rovo P. 2024. Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking
protein. Biomacromolecules. 25(3), 1759–1774.
mla: Wu, Dongqing, et al. “Unveiling the Dynamic Self-Assembly of a Recombinant
Dragline-Silk-Mimicking Protein.” Biomacromolecules, vol. 25, no. 3, American
Chemical Society, 2024, pp. 1759–74, doi:10.1021/acs.biomac.3c01239.
short: D. Wu, A. Koscic, S. Schneider, R.C.A. Dubini, D.C. Rodriguez Camargo, S.
Schneider, P. Rovo, Biomacromolecules 25 (2024) 1759–1774.
corr_author: '1'
date_created: 2024-10-02T10:09:53Z
date_published: 2024-03-11T00:00:00Z
date_updated: 2025-09-08T09:52:18Z
day: '11'
ddc:
- '540'
department:
- _id: NMR
doi: 10.1021/acs.biomac.3c01239
external_id:
isi:
- '001166501000001'
pmid:
- '38343096'
file:
- access_level: open_access
checksum: 9552b6d52f1e8a350764849a535fc13e
content_type: application/pdf
creator: dernst
date_created: 2024-10-07T08:33:35Z
date_updated: 2024-10-07T08:33:35Z
file_id: '18180'
file_name: 2024_BioMacromolecules_Wu.pdf
file_size: 6597227
relation: main_file
success: 1
file_date_updated: 2024-10-07T08:33:35Z
has_accepted_license: '1'
intvolume: ' 25'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 1759-1774
pmid: 1
publication: Biomacromolecules
publication_identifier:
eissn:
- 1526-4602
issn:
- 1525-7797
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking
protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 25
year: '2024'
...
---
OA_type: closed access
_id: '18310'
article_processing_charge: No
article_type: original
author:
- first_name: Maria
full_name: Kitsara, Maria
last_name: Kitsara
- first_name: Merima
full_name: Smajlhodžić-Deljo, Merima
last_name: Smajlhodžić-Deljo
- first_name: Lejla
full_name: Gurbeta Pokvic, Lejla
last_name: Gurbeta Pokvic
- first_name: Bettina
full_name: Bert, Bettina
last_name: Bert
- first_name: Nataliia
full_name: Bubalo, Nataliia
last_name: Bubalo
- first_name: Sevilay
full_name: Erden, Sevilay
last_name: Erden
- first_name: Nuno Henrique
full_name: Franco, Nuno Henrique
last_name: Franco
- first_name: Giuseppe
full_name: Chirico, Giuseppe
last_name: Chirico
- first_name: Jonathan
full_name: Gómez Raja, Jonathan
last_name: Gómez Raja
- first_name: Fernando
full_name: Gonzalez-Uarquin, Fernando
last_name: Gonzalez-Uarquin
- first_name: Annemarie
full_name: Lang, Annemarie
last_name: Lang
- first_name: Nicole
full_name: Linklater, Nicole
last_name: Linklater
- first_name: Sandra
full_name: Mojsova, Sandra
last_name: Mojsova
- first_name: I. Anna S.
full_name: Olsson, I. Anna S.
last_name: Olsson
- first_name: Ioanna
full_name: Sandvig, Ioanna
last_name: Sandvig
- first_name: Alexandra
full_name: Schaffert, Alexandra
last_name: Schaffert
- first_name: Marthe
full_name: Schmit, Marthe
last_name: Schmit
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
- first_name: Bogdan
full_name: Sevastre, Bogdan
last_name: Sevastre
- first_name: Doris
full_name: Wilflingseder, Doris
last_name: Wilflingseder
- first_name: Arti
full_name: Ahluwalia, Arti
last_name: Ahluwalia
- first_name: Winfried
full_name: Neuhaus, Winfried
last_name: Neuhaus
citation:
ama: Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, et al. Introducing the COST
action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).
Alternatives to Laboratory Animals. 2024;52(6):326-333. doi:10.1177/02611929241286024
apa: Kitsara, M., Smajlhodžić-Deljo, M., Gurbeta Pokvic, L., Bert, B., Bubalo, N.,
Erden, S., … Neuhaus, W. (2024). Introducing the COST action ‘Improving the Quality
of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to Laboratory
Animals. SAGE Publications. https://doi.org/10.1177/02611929241286024
chicago: Kitsara, Maria, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina
Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, et al. “Introducing
the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’
(IMPROVE).” Alternatives to Laboratory Animals. SAGE Publications, 2024.
https://doi.org/10.1177/02611929241286024.
ieee: M. Kitsara et al., “Introducing the COST action ‘Improving the Quality
of Biomedical Science with 3Rs Concepts’ (IMPROVE),” Alternatives to Laboratory
Animals, vol. 52, no. 6. SAGE Publications, pp. 326–333, 2024.
ista: Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, Bert B, Bubalo N, Erden
S, Franco NH, Chirico G, Gómez Raja J, Gonzalez-Uarquin F, Lang A, Linklater N,
Mojsova S, Olsson IAS, Sandvig I, Schaffert A, Schmit M, Schober S, Sevastre B,
Wilflingseder D, Ahluwalia A, Neuhaus W. 2024. Introducing the COST action ‘Improving
the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to
Laboratory Animals. 52(6), 326–333.
mla: Kitsara, Maria, et al. “Introducing the COST Action ‘Improving the Quality
of Biomedical Science with 3Rs Concepts’ (IMPROVE).” Alternatives to Laboratory
Animals, vol. 52, no. 6, SAGE Publications, 2024, pp. 326–33, doi:10.1177/02611929241286024.
short: M. Kitsara, M. Smajlhodžić-Deljo, L. Gurbeta Pokvic, B. Bert, N. Bubalo,
S. Erden, N.H. Franco, G. Chirico, J. Gómez Raja, F. Gonzalez-Uarquin, A. Lang,
N. Linklater, S. Mojsova, I.A.S. Olsson, I. Sandvig, A. Schaffert, M. Schmit,
S. Schober, B. Sevastre, D. Wilflingseder, A. Ahluwalia, W. Neuhaus, Alternatives
to Laboratory Animals 52 (2024) 326–333.
date_created: 2024-10-13T22:01:51Z
date_published: 2024-11-01T00:00:00Z
date_updated: 2025-09-08T09:56:39Z
day: '01'
department:
- _id: PreCl
doi: 10.1177/02611929241286024
external_id:
isi:
- '001348633700007'
pmid:
- '39333027'
intvolume: ' 52'
isi: 1
issue: '6'
language:
- iso: eng
month: '11'
oa_version: None
page: 326-333
pmid: 1
publication: Alternatives to Laboratory Animals
publication_identifier:
eissn:
- 2632-3559
issn:
- 0261-1929
publication_status: published
publisher: SAGE Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Introducing the COST action ‘Improving the Quality of Biomedical Science with
3Rs Concepts’ (IMPROVE)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 52
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18581'
abstract:
- lang: eng
text: "Background: Human induced pluripotent stem cells represent a scalable source
of youthful tissue progenitors and secretomes for regenerative therapies. The
aim of our study was to investigate the potential of conditioned medium (CM) from
hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation
of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated
whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could
enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13
donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures
and the effects on proliferation and osteogenic differentiation were examined
after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared
with the activity of MSC-derived CM and with the activity of CM prepared from
hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative
proteomic analysis of CM was performed to gain insight into the molecular components
responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived
MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage
differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline
phosphatase activity of MSC from several adult/aged donors after 14 days of continuous
supplementation under osteogenic conditions. However, CM supplementation failed
to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions.
hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than
CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not
osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity.
Quantitative proteomic analysis of d14-CM revealed a distinct profile of components
that formed a highly interconnected associations network with two clusters, one
functionally associated with binding and organization of actin/cytoskeletal components
and the other with structural constituents of the extracellular matrix, collagen,
and growth factor binding. Several hub proteins were identified that were reported
to have functions in cell-extracellular matrix interaction, osteogenic differentiation
and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early
osteogenic differentiation of human bone marrow-derived MSCs and that prolonged
cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis
of the upregulated protein components provides the basis for further optimization
of hiPSC-MP-CM for bone regenerative therapies."
acknowledgement: "We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for
providing the human tissue waste for primary cell isolation and the New York Stem
Cell Foundation Research Institute for providing the human induced pluripotent stem
cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues
at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing
support of the project. InstaText writing tool (https://instatext.io) was used to
edit the English language of the final manuscript.\r\nThis work has received funding
from the European Union’s Horizon 2020 research and innovation program under the
Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming
European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well
as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and
853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951)
and the Production of the Future program (grant agreement No. 877452)."
article_number: '434'
article_processing_charge: Yes
article_type: original
author:
- first_name: Darja
full_name: Marolt Presen, Darja
last_name: Marolt Presen
- first_name: Vanessa
full_name: Goeschl, Vanessa
last_name: Goeschl
- first_name: Dominik
full_name: Hanetseder, Dominik
last_name: Hanetseder
- first_name: Laura
full_name: Ogrin, Laura
last_name: Ogrin
- first_name: Alexandra Larissa
full_name: Stetco, Alexandra Larissa
last_name: Stetco
- first_name: Anja
full_name: Tansek, Anja
last_name: Tansek
- first_name: Laura
full_name: Pozenel, Laura
last_name: Pozenel
- first_name: Bella
full_name: Bruszel, Bella
id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
last_name: Bruszel
- first_name: Goran
full_name: Mitulovic, Goran
last_name: Mitulovic
- first_name: Johannes
full_name: Oesterreicher, Johannes
last_name: Oesterreicher
- first_name: Johannes
full_name: Zipperle, Johannes
last_name: Zipperle
- first_name: Barbara
full_name: Schaedl, Barbara
last_name: Schaedl
- first_name: Wolfgang
full_name: Holnthoner, Wolfgang
last_name: Holnthoner
- first_name: Johannes
full_name: Grillari, Johannes
last_name: Grillari
- first_name: Heinz
full_name: Redl, Heinz
last_name: Redl
citation:
ama: Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances
the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium.
Stem Cell Research and Therapy. 2024;15. doi:10.1186/s13287-024-03960-5
apa: Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek,
A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity
of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research
and Therapy. Springer Nature. https://doi.org/10.1186/s13287-024-03960-5
chicago: Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin,
Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation
Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned
Medium.” Stem Cell Research and Therapy. Springer Nature, 2024. https://doi.org/10.1186/s13287-024-03960-5.
ieee: D. Marolt Presen et al., “Prolonged cultivation enhances the stimulatory
activity of hiPSC mesenchymal progenitor-derived conditioned medium,” Stem
Cell Research and Therapy, vol. 15. Springer Nature, 2024.
ista: Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel
L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner
W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity
of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research
and Therapy. 15, 434.
mla: Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory
Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” Stem
Cell Research and Therapy, vol. 15, 434, Springer Nature, 2024, doi:10.1186/s13287-024-03960-5.
short: D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek,
L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl,
W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).
date_created: 2024-11-24T23:01:47Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2025-09-09T11:41:12Z
day: '01'
ddc:
- '570'
department:
- _id: LifeSc
doi: 10.1186/s13287-024-03960-5
external_id:
isi:
- '001356479400001'
pmid:
- '39551765'
file:
- access_level: open_access
checksum: 91edba8edde30d781dce89fdd5cadc39
content_type: application/pdf
creator: dernst
date_created: 2024-12-10T08:28:17Z
date_updated: 2024-12-10T08:28:17Z
file_id: '18641'
file_name: 2024_StemCellResearch_Presen.pdf
file_size: 6690494
relation: main_file
success: 1
file_date_updated: 2024-12-10T08:28:17Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Stem Cell Research and Therapy
publication_identifier:
eissn:
- 1757-6512
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal
progenitor-derived conditioned medium
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18587'
abstract:
- lang: eng
text: Many scientific breakthroughs have depended on animal research, yet the ethical
concerns surrounding the use of animals in experimentation have long prompted
discussions about humane treatment and responsible scientific practice. First
articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction,
and Refinement have gained widespread recognition as basic guidelines for animal
research. Over time, the 3Rs have transcended the research community, influencing
policy decisions, animal welfare advocacy and public perception of animal experimentation.
Despite their broad acceptance, interpretations of the 3Rs vary substantially,
shaping statutory frameworks at various levels, with both technical and practical
impacts.
acknowledgement: This publication is based upon work from the Ethics Crossover Group
within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical
science”), CA21139, supported by COST (European Cooperation in Science and Technology).
We acknowledge the input and advice from Dr. Susanna Louhimies.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Jan
full_name: Lauwereyns, Jan
last_name: Lauwereyns
- first_name: Jeffrey
full_name: Bajramovic, Jeffrey
last_name: Bajramovic
- first_name: Bettina
full_name: Bert, Bettina
last_name: Bert
- first_name: Samuel
full_name: Camenzind, Samuel
last_name: Camenzind
- first_name: Joery
full_name: De Kock, Joery
last_name: De Kock
- first_name: Alisa
full_name: Elezović, Alisa
last_name: Elezović
- first_name: Sevilay
full_name: Erden, Sevilay
last_name: Erden
- first_name: Fernando
full_name: Gonzalez-Uarquin, Fernando
last_name: Gonzalez-Uarquin
- first_name: Yesim Isil
full_name: Ulman, Yesim Isil
last_name: Ulman
- first_name: Orsolya Ivett
full_name: Hoffmann, Orsolya Ivett
last_name: Hoffmann
- first_name: Maria
full_name: Kitsara, Maria
last_name: Kitsara
- first_name: Nikolaos
full_name: Kostomitsopoulos, Nikolaos
last_name: Kostomitsopoulos
- first_name: Winfried
full_name: Neuhaus, Winfried
last_name: Neuhaus
- first_name: Benoit
full_name: Petit-Demouliere, Benoit
last_name: Petit-Demouliere
- first_name: Simone
full_name: Pollo, Simone
last_name: Pollo
- first_name: Brígida
full_name: Riso, Brígida
last_name: Riso
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
- first_name: Athanassia
full_name: Sotiropoulos, Athanassia
last_name: Sotiropoulos
- first_name: Aurélie
full_name: Thomas, Aurélie
last_name: Thomas
- first_name: Augusto
full_name: Vitale, Augusto
last_name: Vitale
- first_name: Doris
full_name: Wilflingseder, Doris
last_name: Wilflingseder
- first_name: Arti
full_name: Ahluwalia, Arti
last_name: Ahluwalia
citation:
ama: Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of
the 3Rs principles in animal research. Lab Animal. 2024;53:347-350. doi:10.1038/s41684-024-01476-2
apa: Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović,
A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles
in animal research. Lab Animal. Springer Nature. https://doi.org/10.1038/s41684-024-01476-2
chicago: Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery
De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation
of the 3Rs Principles in Animal Research.” Lab Animal. Springer Nature,
2024. https://doi.org/10.1038/s41684-024-01476-2.
ieee: J. Lauwereyns et al., “Toward a common interpretation of the 3Rs principles
in animal research,” Lab Animal, vol. 53. Springer Nature, pp. 347–350,
2024.
ista: Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden
S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus
W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale
A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs
principles in animal research. Lab Animal. 53, 347–350.
mla: Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles
in Animal Research.” Lab Animal, vol. 53, Springer Nature, 2024, pp. 347–50,
doi:10.1038/s41684-024-01476-2.
short: J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović,
S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos,
W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos,
A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.
date_created: 2024-11-24T23:01:49Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2025-09-08T14:50:31Z
day: '01'
ddc:
- '570'
department:
- _id: PreCl
doi: 10.1038/s41684-024-01476-2
external_id:
isi:
- '001355264100001'
pmid:
- '39548348'
file:
- access_level: open_access
checksum: 67fc140f761581a291591f075e49b88d
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T14:07:04Z
date_updated: 2024-12-03T14:07:04Z
file_id: '18614'
file_name: 2024_LabAnimal_Lauwereyns.pdf
file_size: 967252
relation: main_file
success: 1
file_date_updated: 2024-12-03T14:07:04Z
has_accepted_license: '1'
intvolume: ' 53'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 347-350
pmid: 1
publication: Lab Animal
publication_identifier:
eissn:
- 1548-4475
issn:
- 0093-7355
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Toward a common interpretation of the 3Rs principles in animal research
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 53
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18920'
abstract:
- lang: eng
text: The globally distributed marine alga Emiliania huxleyi has cooling effect
on the Earth’s climate. The population density of E. huxleyi is restricted by
Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite
the impact of E. huxleyi viruses on the climate, there is limited information
about their structure and replication. Here, we show that the dsDNA genome inside
the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane.
EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse
the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble
in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments.
Genome packaging initiates synchronously with the capsid assembly and completes
through an aperture in the forming capsid. The genome-filled capsids acquire an
outer membrane by budding into intracellular vesicles. EhV-201 infection induces
a loss of surface protective layers from E. huxleyi cells, which enables the continuous
release of virions by exocytosis.
acknowledgement: We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core
Facility and Proteomics Core Facility of the Central European Institute of Technology
(CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and
Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility
supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR);
and (iii) Plant Sciences Core Facility for support with obtaining scientific data
presented here. We acknowledge support from the project National Institute of Virology
and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European
Union - Next Generation EU. This work received funding from the Czech Science Foundation
grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI”
(no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship
funded by Brno city municipality to M.H.
article_number: 'eadk1954 '
article_processing_charge: Yes
article_type: original
author:
- first_name: Miroslav
full_name: Homola, Miroslav
last_name: Homola
- first_name: Renate Carina
full_name: Büttner, Renate Carina
id: 3b7984c9-17ff-11ed-b6fe-f943c4a5b626
last_name: Büttner
- first_name: Tibor
full_name: Füzik, Tibor
last_name: Füzik
- first_name: Pavel
full_name: Křepelka, Pavel
last_name: Křepelka
- first_name: Radka
full_name: Holbová, Radka
last_name: Holbová
- first_name: Jiří
full_name: Nováček, Jiří
last_name: Nováček
- first_name: Marten L.
full_name: Chaillet, Marten L.
last_name: Chaillet
- first_name: Jakub
full_name: Žák, Jakub
last_name: Žák
- first_name: Danyil
full_name: Grybchuk, Danyil
last_name: Grybchuk
- first_name: Friedrich
full_name: Förster, Friedrich
last_name: Förster
- first_name: William H.
full_name: Wilson, William H.
last_name: Wilson
- first_name: Declan C.
full_name: Schroeder, Declan C.
last_name: Schroeder
- first_name: Pavel
full_name: Plevka, Pavel
last_name: Plevka
citation:
ama: Homola M, Büttner RC, Füzik T, et al. Structure and replication cycle of a
virus infecting climate-modulating alga Emiliania huxleyi. Science Advances.
2024;10(15). doi:10.1126/sciadv.adk1954
apa: Homola, M., Büttner, R. C., Füzik, T., Křepelka, P., Holbová, R., Nováček,
J., … Plevka, P. (2024). Structure and replication cycle of a virus infecting
climate-modulating alga Emiliania huxleyi. Science Advances. American Association
for the Advancement of Science. https://doi.org/10.1126/sciadv.adk1954
chicago: Homola, Miroslav, Renate Carina Büttner, Tibor Füzik, Pavel Křepelka, Radka
Holbová, Jiří Nováček, Marten L. Chaillet, et al. “Structure and Replication Cycle
of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” Science Advances.
American Association for the Advancement of Science, 2024. https://doi.org/10.1126/sciadv.adk1954.
ieee: M. Homola et al., “Structure and replication cycle of a virus infecting
climate-modulating alga Emiliania huxleyi,” Science Advances, vol. 10,
no. 15. American Association for the Advancement of Science, 2024.
ista: Homola M, Büttner RC, Füzik T, Křepelka P, Holbová R, Nováček J, Chaillet
ML, Žák J, Grybchuk D, Förster F, Wilson WH, Schroeder DC, Plevka P. 2024. Structure
and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi.
Science Advances. 10(15), eadk1954.
mla: Homola, Miroslav, et al. “Structure and Replication Cycle of a Virus Infecting
Climate-Modulating Alga Emiliania Huxleyi.” Science Advances, vol. 10,
no. 15, eadk1954, American Association for the Advancement of Science, 2024, doi:10.1126/sciadv.adk1954.
short: M. Homola, R.C. Büttner, T. Füzik, P. Křepelka, R. Holbová, J. Nováček, M.L.
Chaillet, J. Žák, D. Grybchuk, F. Förster, W.H. Wilson, D.C. Schroeder, P. Plevka,
Science Advances 10 (2024).
date_created: 2025-01-27T14:32:34Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2025-05-14T09:29:04Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.1126/sciadv.adk1954
external_id:
pmid:
- '38598627'
file:
- access_level: open_access
checksum: 291dd7ceccbe6bfd8e0a9157584f88e9
content_type: application/pdf
creator: dernst
date_created: 2025-01-27T14:40:08Z
date_updated: 2025-01-27T14:40:08Z
file_id: '18921'
file_name: 2024_ScienceAdv_Homola.pdf
file_size: 40623405
relation: main_file
success: 1
file_date_updated: 2025-01-27T14:40:08Z
has_accepted_license: '1'
intvolume: ' 10'
issue: '15'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
link:
- relation: software
url: ' https://github.com/fuzikt/tomostarpy.'
scopus_import: '1'
status: public
title: Structure and replication cycle of a virus infecting climate-modulating alga
Emiliania huxleyi
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '19520'
abstract:
- lang: eng
text: Vertebrates exhibit a wide range of motor behaviors, ranging from swimming
to complex limb-based movements. Here we take advantage of frog metamorphosis,
which captures a swim-to-limb-based movement transformation during the development
of a single organism, to explore changes in the underlying spinal circuits. We
find that the tadpole spinal cord contains small and largely homogeneous populations
of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages.
These neuronal populations only modestly increase in number and subtype heterogeneity
with the emergence of free swimming. In contrast, during frog metamorphosis and
the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron
number and transcriptional heterogeneity, culminating in cohorts of neurons that
exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated
gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively,
results in severe but selective deficits in tail and limb function. Our work thus
demonstrates that neural diversity scales exponentially with increasing behavioral
complexity and illustrates striking evolutionary conservation in the molecular
organization and function of motor circuits across species.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We would like to thank the members of the Sweeney Lab (especially
Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project
and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion,
and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their
scientific insight and mentorship during the conception of this project. This\r\nproject
would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena
Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA).
In addition, we thank our funding sources for providing the resources to do these\r\nexperiments:
FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon
Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch
Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS
5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.);
NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities
(ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472
(Z.H.); and Project A.L.S. (S.B-M.). "
article_processing_charge: No
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales
exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv.
doi:10.1101/2024.09.20.614050
apa: Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R.,
Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis. bioRxiv. https://doi.org/10.1101/2024.09.20.614050
chicago: Vijatovic, David, Florina Alexandra Toma, Zoe P Harrington, Christoph
M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal
Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during
Frog Metamorphosis.” BioRxiv, n.d. https://doi.org/10.1101/2024.09.20.614050.
ieee: D. Vijatovic et al., “Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis,” bioRxiv. .
ista: Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ,
Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney
LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis. bioRxiv, 10.1101/2024.09.20.614050.
mla: Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with
Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, doi:10.1101/2024.09.20.614050.
short: D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J.
Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen,
J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2025-04-07T08:48:28Z
date_published: 2024-09-27T00:00:00Z
date_updated: 2025-05-14T11:40:13Z
day: '27'
department:
- _id: LoSw
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1101/2024.09.20.614050
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.09.20.614050
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: bioRxiv
publication_status: submitted
status: public
title: Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '14795'
abstract:
- lang: eng
text: Metazoan development relies on the formation and remodeling of cell-cell contacts.
Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in
space and time plays a central role in cell-cell contact formation and maturation.
Nevertheless, how this process is mechanistically achieved when new contacts are
formed remains unclear. Here, by building a biomimetic assay composed of progenitor
cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains,
we show that cortical F-actin flows, driven by the depletion of myosin-2 at the
cell contact center, mediate the dynamic reorganization of adhesion receptors
and cell cortex at the contact. E-cadherin-dependent downregulation of the small
GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a
decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2
becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical
tension gradient from the contact rim to its center. This tension gradient, in
turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin
at the contact rim and the progressive redistribution of E-cadherin from the contact
center to the rim. Eventually, this combination of actomyosin downregulation and
flows at the contact determines the characteristic molecular organization, with
E-cadherin and F-actin accumulating at the contact rim, where they are needed
to mechanically link the contractile cortices of the adhering cells.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "We are grateful to Edwin Munro for their feedback and help with
the single particle analysis. We thank members of the Heisenberg and Loose labs
for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH
plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA
for their continuous support, especially Yann Cesbron for assistance with the laser
cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Feyza N
full_name: Arslan, Feyza N
id: 49DA7910-F248-11E8-B48F-1D18A9856A87
last_name: Arslan
orcid: 0000-0001-5809-9566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology.
2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067
apa: Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P.
J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts.
Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067
chicago: Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp
J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell
Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.
ieee: F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg,
“Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current
Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.
ista: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1),
171–182.e8.
mla: Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated
Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8,
doi:10.1016/j.cub.2023.11.067.
short: F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current
Biology 34 (2024) 171–182.e8.
corr_author: '1'
date_created: 2024-01-14T23:00:56Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2025-09-04T11:39:10Z
day: '08'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MaLo
- _id: NanoFab
doi: 10.1016/j.cub.2023.11.067
ec_funded: 1
external_id:
isi:
- '001154500400001'
pmid:
- '38134934'
file:
- access_level: open_access
checksum: 51220b76d72a614208f84bdbfbaf9b72
content_type: application/pdf
creator: dernst
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project:
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call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2024'
...
---
_id: '14846'
abstract:
- lang: eng
text: Contraction and flow of the actin cell cortex have emerged as a common principle
by which cells reorganize their cytoplasm and take shape. However, how these cortical
flows interact with adjacent cytoplasmic components, changing their form and localization,
and how this affects cytoplasmic organization and cell shape remains unclear.
Here we show that in ascidian oocytes, the cooperative activities of cortical
actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive
oocyte cytoplasmic reorganization and shape changes following fertilization. We
show that vegetal-directed cortical actomyosin flows, established upon oocyte
fertilization, lead to both the accumulation of cortical actin at the vegetal
pole of the zygote and compression and local buckling of the adjacent elastic
solid-like myoplasm layer due to friction forces generated at their interface.
Once cortical flows have ceased, the multiple myoplasm buckles resolve into one
larger buckle, which again drives the formation of the contraction pole—a protuberance
of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings
reveal a mechanism where cortical actomyosin network flows determine cytoplasmic
reorganization and cell shape by deforming adjacent cytoplasmic components through
friction forces.
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: NanoFab
acknowledgement: We would like to thank A. McDougall, E. Hannezo and the Heisenberg
lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP
and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific
Service Units of the Institute of Science and Technology Austria through resources
provided by the Electron Microscopy Facility, Imaging and Optics Facility and the
Nanofabrication Facility. This work was supported by a Joint Project Grant from
the FWF (I 3601-B27).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Silvia
full_name: Caballero Mancebo, Silvia
id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
last_name: Caballero Mancebo
orcid: 0000-0002-5223-3346
- first_name: Rushikesh
full_name: Shinde, Rushikesh
last_name: Shinde
- first_name: Madison
full_name: Bolger-Munro, Madison
id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
last_name: Bolger-Munro
orcid: 0000-0002-8176-4824
- first_name: Matilda
full_name: Peruzzo, Matilda
id: 3F920B30-F248-11E8-B48F-1D18A9856A87
last_name: Peruzzo
orcid: 0000-0002-3415-4628
- first_name: Gregory
full_name: Szep, Gregory
id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
last_name: Szep
- first_name: Irene
full_name: Steccari, Irene
id: 2705C766-9FE2-11EA-B224-C6773DDC885E
last_name: Steccari
- first_name: David
full_name: Labrousse Arias, David
id: CD573DF4-9ED3-11E9-9D77-3223E6697425
last_name: Labrousse Arias
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Andrew
full_name: Callan-Jones, Andrew
last_name: Callan-Jones
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine
cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.
Nature Physics. 2024;20:310-321. doi:10.1038/s41567-023-02302-1
apa: Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G.,
Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic
reorganization and shape changes of ascidian oocytes upon fertilization. Nature
Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02302-1
chicago: Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda
Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction
Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes
upon Fertilization.” Nature Physics. Springer Nature, 2024. https://doi.org/10.1038/s41567-023-02302-1.
ieee: S. Caballero Mancebo et al., “Friction forces determine cytoplasmic
reorganization and shape changes of ascidian oocytes upon fertilization,” Nature
Physics, vol. 20. Springer Nature, pp. 310–321, 2024.
ista: Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari
I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg
C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes
of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.
mla: Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization
and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics,
vol. 20, Springer Nature, 2024, pp. 310–21, doi:10.1038/s41567-023-02302-1.
short: S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I.
Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez,
C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.
corr_author: '1'
date_created: 2024-01-21T23:00:57Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T11:48:28Z
day: '01'
ddc:
- '530'
department:
- _id: CaHe
- _id: JoFi
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
doi: 10.1038/s41567-023-02302-1
external_id:
isi:
- '001138880800005'
pmid:
- '38370025'
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language:
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month: '02'
oa: 1
oa_version: Published Version
page: 310-321
pmid: 1
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03601
name: Control of embryonic cleavage pattern
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/
scopus_import: '1'
status: public
title: Friction forces determine cytoplasmic reorganization and shape changes of ascidian
oocytes upon fertilization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...
---
_id: '14926'
author:
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
citation:
ama: Hauschild R. Matlab script for analysis of clone dispersal. 2024. doi:10.15479/AT:ISTA:14926
apa: Hauschild, R. (2024). Matlab script for analysis of clone dispersal. Institute
of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14926
chicago: Hauschild, Robert. “Matlab Script for Analysis of Clone Dispersal.” Institute
of Science and Technology Austria, 2024. https://doi.org/10.15479/AT:ISTA:14926.
ieee: R. Hauschild, “Matlab script for analysis of clone dispersal.” Institute of
Science and Technology Austria, 2024.
ista: Hauschild R. 2024. Matlab script for analysis of clone dispersal, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:14926.
mla: Hauschild, Robert. Matlab Script for Analysis of Clone Dispersal. Institute
of Science and Technology Austria, 2024, doi:10.15479/AT:ISTA:14926.
short: R. Hauschild, (2024).
corr_author: '1'
date_created: 2024-02-02T14:42:26Z
date_published: 2024-02-02T00:00:00Z
date_updated: 2025-09-04T12:10:39Z
day: '02'
ddc:
- '570'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:14926
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file_name: Supplementary_file_1.zip
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has_accepted_license: '1'
license: https://opensource.org/licenses/MIT
month: '02'
oa: 1
publisher: Institute of Science and Technology Austria
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status: public
title: Matlab script for analysis of clone dispersal
tmp:
legal_code_url: https://opensource.org/licenses/MIT
name: The MIT License
short: MIT
type: software
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '15018'
abstract:
- lang: eng
text: The epitaxial growth of a strained Ge layer, which is a promising candidate
for the channel material of a hole spin qubit, has been demonstrated on 300 mm
Si wafers using commercially available Si0.3Ge0.7 strain relaxed buffer (SRB)
layers. The assessment of the layer and the interface qualities for a buried strained
Ge layer embedded in Si0.3Ge0.7 layers is reported. The XRD reciprocal space mapping
confirmed that the reduction of the growth temperature enables the 2-dimensional
growth of the Ge layer fully strained with respect to the Si0.3Ge0.7. Nevertheless,
dislocations at the top and/or bottom interface of the Ge layer were observed
by means of electron channeling contrast imaging, suggesting the importance of
the careful dislocation assessment. The interface abruptness does not depend on
the selection of the precursor gases, but it is strongly influenced by the growth
temperature which affects the coverage of the surface H-passivation. The mobility
of 2.7 × 105 cm2/Vs is promising, while the low percolation density of 3 × 1010
/cm2 measured with a Hall-bar device at 7 K illustrates the high quality of the
heterostructure thanks to the high Si0.3Ge0.7 SRB quality.
acknowledgement: The Ge project received funding from the European Union's Horizon
Europe programme under the Grant Agreement 101069515 – IGNITE. Siltronic AG is acknowledged
for providing the SRB wafers. This work was supported by Imec's Industrial Affiliation
Program on Quantum Computing.
article_number: '108231'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Yosuke
full_name: Shimura, Yosuke
last_name: Shimura
- first_name: Clement
full_name: Godfrin, Clement
last_name: Godfrin
- first_name: Andriy
full_name: Hikavyy, Andriy
last_name: Hikavyy
- first_name: Roy
full_name: Li, Roy
last_name: Li
- first_name: Juan L
full_name: Aguilera Servin, Juan L
id: 2A67C376-F248-11E8-B48F-1D18A9856A87
last_name: Aguilera Servin
orcid: 0000-0002-2862-8372
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
- first_name: Paola
full_name: Favia, Paola
last_name: Favia
- first_name: Han
full_name: Han, Han
last_name: Han
- first_name: Danny
full_name: Wan, Danny
last_name: Wan
- first_name: Kristiaan
full_name: de Greve, Kristiaan
last_name: de Greve
- first_name: Roger
full_name: Loo, Roger
last_name: Loo
citation:
ama: Shimura Y, Godfrin C, Hikavyy A, et al. Compressively strained epitaxial Ge
layers for quantum computing applications. Materials Science in Semiconductor
Processing. 2024;174(5). doi:10.1016/j.mssp.2024.108231
apa: Shimura, Y., Godfrin, C., Hikavyy, A., Li, R., Aguilera Servin, J. L., Katsaros,
G., … Loo, R. (2024). Compressively strained epitaxial Ge layers for quantum computing
applications. Materials Science in Semiconductor Processing. Elsevier.
https://doi.org/10.1016/j.mssp.2024.108231
chicago: Shimura, Yosuke, Clement Godfrin, Andriy Hikavyy, Roy Li, Juan L Aguilera
Servin, Georgios Katsaros, Paola Favia, et al. “Compressively Strained Epitaxial
Ge Layers for Quantum Computing Applications.” Materials Science in Semiconductor
Processing. Elsevier, 2024. https://doi.org/10.1016/j.mssp.2024.108231.
ieee: Y. Shimura et al., “Compressively strained epitaxial Ge layers for
quantum computing applications,” Materials Science in Semiconductor Processing,
vol. 174, no. 5. Elsevier, 2024.
ista: Shimura Y, Godfrin C, Hikavyy A, Li R, Aguilera Servin JL, Katsaros G, Favia
P, Han H, Wan D, de Greve K, Loo R. 2024. Compressively strained epitaxial Ge
layers for quantum computing applications. Materials Science in Semiconductor
Processing. 174(5), 108231.
mla: Shimura, Yosuke, et al. “Compressively Strained Epitaxial Ge Layers for Quantum
Computing Applications.” Materials Science in Semiconductor Processing,
vol. 174, no. 5, 108231, Elsevier, 2024, doi:10.1016/j.mssp.2024.108231.
short: Y. Shimura, C. Godfrin, A. Hikavyy, R. Li, J.L. Aguilera Servin, G. Katsaros,
P. Favia, H. Han, D. Wan, K. de Greve, R. Loo, Materials Science in Semiconductor
Processing 174 (2024).
date_created: 2024-02-22T14:10:40Z
date_published: 2024-05-20T00:00:00Z
date_updated: 2025-04-14T08:01:27Z
day: '20'
ddc:
- '530'
department:
- _id: GeKa
- _id: NanoFab
doi: 10.1016/j.mssp.2024.108231
external_id:
isi:
- '001188520000001'
file:
- access_level: open_access
checksum: 62e8e9ae960387a3dca32ec7f5e413ab
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T11:56:08Z
date_updated: 2024-07-22T11:56:08Z
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success: 1
file_date_updated: 2024-07-22T11:56:08Z
has_accepted_license: '1'
intvolume: ' 174'
isi: 1
issue: '5'
keyword:
- Mechanical Engineering
- Mechanics of Materials
- Condensed Matter Physics
- General Materials Science
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 34c0acea-11ca-11ed-8bc3-8775e10fd452
grant_number: '101069515'
name: Integrated Germanium Quantum Technology
publication: Materials Science in Semiconductor Processing
publication_identifier:
issn:
- 1369-8001
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Compressively strained epitaxial Ge layers for quantum computing applications
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 174
year: '2024'
...