---
_id: '825'
abstract:
- lang: eng
  text: What data is needed about data? Describing the process to answer this question
    for the institutional data repository IST DataRep.
article_processing_charge: No
author:
- first_name: Barbara
  full_name: Petritsch, Barbara
  id: 406048EC-F248-11E8-B48F-1D18A9856A87
  last_name: Petritsch
  orcid: 0000-0003-2724-4614
citation:
  ama: Petritsch B. Metadata for research data in practice. <i>Mitteilungen der Vereinigung
    Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. 2017;70(2):200-207.
    doi:<a href="https://doi.org/10.31263/voebm.v70i2.1678">10.31263/voebm.v70i2.1678</a>
  apa: Petritsch, B. (2017). Metadata for research data in practice. <i>Mitteilungen
    Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. VÖB.
    <a href="https://doi.org/10.31263/voebm.v70i2.1678">https://doi.org/10.31263/voebm.v70i2.1678</a>
  chicago: Petritsch, Barbara. “Metadata for Research Data in Practice.” <i>Mitteilungen
    Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>. VÖB,
    2017. <a href="https://doi.org/10.31263/voebm.v70i2.1678">https://doi.org/10.31263/voebm.v70i2.1678</a>.
  ieee: B. Petritsch, “Metadata for research data in practice,” <i>Mitteilungen der
    Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>, vol. 70,
    no. 2. VÖB, pp. 200–207, 2017.
  ista: Petritsch B. 2017. Metadata for research data in practice. Mitteilungen der
    Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare. 70(2), 200–207.
  mla: Petritsch, Barbara. “Metadata for Research Data in Practice.” <i>Mitteilungen
    Der Vereinigung Österreichischer Bibliothekarinnen &#38; Bibliothekare</i>, vol.
    70, no. 2, VÖB, 2017, pp. 200–07, doi:<a href="https://doi.org/10.31263/voebm.v70i2.1678">10.31263/voebm.v70i2.1678</a>.
  short: B. Petritsch, Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen
    &#38; Bibliothekare 70 (2017) 200–207.
corr_author: '1'
date_created: 2018-12-11T11:48:42Z
date_published: 2017-08-01T00:00:00Z
date_updated: 2025-07-10T11:55:09Z
day: '01'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v70i2.1678
file:
- access_level: open_access
  checksum: 7c4544d07efa2c2add8612b489abb4e2
  content_type: application/pdf
  creator: dernst
  date_created: 2019-01-18T13:32:17Z
  date_updated: 2020-07-14T12:48:11Z
  file_id: '5850'
  file_name: 2017_VOEB_Petritsch.pdf
  file_size: 7843975
  relation: main_file
file_date_updated: 2020-07-14T12:48:11Z
has_accepted_license: '1'
intvolume: '        70'
issue: '2'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '08'
oa: 1
oa_version: Published Version
page: 200 - 207
publication: Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare
publication_identifier:
  issn:
  - 1022-2588
publication_status: published
publisher: VÖB
publist_id: '6823'
scopus_import: '1'
status: public
title: Metadata for research data in practice
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 70
year: '2017'
...
---
_id: '946'
abstract:
- lang: eng
  text: Roots navigate through soil integrating environmental signals to orient their
    growth. The Arabidopsis root is a widely used model for developmental, physiological
    and cell biological studies. Live imaging greatly aids these efforts, but the
    horizontal sample position and continuous root tip displacement present significant
    difficulties. Here, we develop a confocal microscope setup for vertical sample
    mounting and integrated directional illumination. We present TipTracker – a custom
    software for automatic tracking of diverse moving objects usable on various microscope
    setups. Combined, this enables observation of root tips growing along the natural
    gravity vector over prolonged periods of time, as well as the ability to induce
    rapid gravity or light stimulation. We also track migrating cells in the developing
    zebrafish embryo, demonstrating the utility of this system in the acquisition
    of high-resolution data sets of dynamic samples. We provide detailed descriptions
    of the tools enabling the easy implementation on other microscopes.
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: "Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel
  von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian
  Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013
  no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop
  at IST Austria for their contribution to the microscope setup and to Yvonne Kemper
  for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility"
article_number: e26792
article_processing_charge: Yes
author:
- first_name: Daniel
  full_name: Von Wangenheim, Daniel
  id: 49E91952-F248-11E8-B48F-1D18A9856A87
  last_name: Von Wangenheim
  orcid: 0000-0002-6862-1247
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live
    tracking of moving samples in confocal microscopy for vertically grown roots.
    <i>eLife</i>. 2017;6. doi:<a href="https://doi.org/10.7554/eLife.26792">10.7554/eLife.26792</a>
  apa: von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38;
    Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically
    grown roots. <i>ELife</i>. eLife Sciences Publications. <a href="https://doi.org/10.7554/eLife.26792">https://doi.org/10.7554/eLife.26792</a>
  chicago: Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone,
    Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy
    for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017.
    <a href="https://doi.org/10.7554/eLife.26792">https://doi.org/10.7554/eLife.26792</a>.
  ieee: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J.
    Friml, “Live tracking of moving samples in confocal microscopy for vertically
    grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.
  ista: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017.
    Live tracking of moving samples in confocal microscopy for vertically grown roots.
    eLife. 6, e26792.
  mla: von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal
    Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences
    Publications, 2017, doi:<a href="https://doi.org/10.7554/eLife.26792">10.7554/eLife.26792</a>.
  short: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml,
    ELife 6 (2017).
date_created: 2018-12-11T11:49:21Z
date_published: 2017-06-19T00:00:00Z
date_updated: 2025-04-15T06:37:26Z
day: '19'
ddc:
- '570'
department:
- _id: JiFr
- _id: Bio
- _id: CaHe
- _id: EvBe
doi: 10.7554/eLife.26792
ec_funded: 1
external_id:
  isi:
  - '000404728300001'
file:
- access_level: open_access
  checksum: 9af3398cb0d81f99d79016a616df22e9
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:17:57Z
  date_updated: 2020-07-14T12:48:15Z
  file_id: '5315'
  file_name: IST-2017-847-v1+1_elife-26792-v2.pdf
  file_size: 19581847
  relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 2572ED28-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: M02128
  name: Molecular basis of root growth inhibition by auxin
- _id: 2542D156-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 1774-B16
  name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '6471'
pubrep_id: '847'
quality_controlled: '1'
related_material:
  record:
  - id: '5566'
    relation: popular_science
    status: public
scopus_import: '1'
status: public
title: Live tracking of moving samples in confocal microscopy for vertically grown
  roots
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 6
year: '2017'
...
---
OA_place: publisher
OA_type: gold
_id: '5450'
abstract:
- lang: eng
  text: 'In this report the implementation of the institutional data repository IST
    DataRep at IST Austria will be covered: Starting with the research phase when
    requirements for a repository were established, the procedure of choosing a repository-software
    and its customization based on the results of user-testings will be discussed.
    Followed by reflections on the marketing strategies in regard of impact, and at
    the end sharing some experiences of one year operating IST DataRep.'
article_processing_charge: No
author:
- first_name: Barbara
  full_name: Petritsch, Barbara
  id: 406048EC-F248-11E8-B48F-1D18A9856A87
  last_name: Petritsch
  orcid: 0000-0003-2724-4614
citation:
  ama: Petritsch B. <i>Implementing the Institutional Data Repository IST DataRep</i>.
    Institute of Science and Technology Austria; 2017.
  apa: Petritsch, B. (2017). <i>Implementing the institutional data repository IST
    DataRep</i>. Institute of Science and Technology Austria.
  chicago: Petritsch, Barbara. <i>Implementing the Institutional Data Repository IST
    DataRep</i>. Institute of Science and Technology Austria, 2017.
  ieee: B. Petritsch, <i>Implementing the institutional data repository IST DataRep</i>.
    Institute of Science and Technology Austria, 2017.
  ista: Petritsch B. 2017. Implementing the institutional data repository IST DataRep,
    Institute of Science and Technology Austria,p.
  mla: Petritsch, Barbara. <i>Implementing the Institutional Data Repository IST DataRep</i>.
    Institute of Science and Technology Austria, 2017.
  short: B. Petritsch, Implementing the Institutional Data Repository IST DataRep,
    Institute of Science and Technology Austria, 2017.
corr_author: '1'
date_created: 2018-12-12T11:39:24Z
date_published: 2017-06-26T00:00:00Z
date_updated: 2025-07-10T11:52:50Z
day: '26'
ddc:
- '020'
department:
- _id: E-Lib
file:
- access_level: open_access
  checksum: 6321792dcfa82bf490f17615a9b22355
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T11:53:22Z
  date_updated: 2020-07-14T12:46:59Z
  file_id: '5483'
  file_name: IST-2017-724-v1+1_DataRep_Project_Report_2017.pdf
  file_size: 3460985
  relation: main_file
file_date_updated: 2020-07-14T12:46:59Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication_status: published
publisher: Institute of Science and Technology Austria
pubrep_id: '724'
status: public
title: Implementing the institutional data repository IST DataRep
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: report
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '5560'
abstract:
- lang: eng
  text: "This repository contains the data collected for the manuscript \"Biased partitioning
    of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity\".\r\nThe
    data is compressed into a single archive. Within the archive, different folders
    correspond to figures of the main text and the SI of the related publication.\r\nData
    is saved as plain text, with each folder containing a separate readme file describing
    the format. Typically, the data is from fluorescence microscopy measurements of
    single cells growing in a microfluidic \"mother machine\" device, and consists
    of relevant values (primarily arbitrary unit or normalized fluorescence measurements,
    and division times / growth rates) after raw microscopy images have been processed,
    segmented, and their features extracted, as described in the methods section of
    the related publication."
article_processing_charge: No
author:
- first_name: Tobias
  full_name: Bergmiller, Tobias
  id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
  last_name: Bergmiller
  orcid: 0000-0001-5396-4346
- first_name: Anna M
  full_name: Andersson, Anna M
  id: 2B8A40DA-F248-11E8-B48F-1D18A9856A87
  last_name: Andersson
  orcid: 0000-0003-2912-6769
- first_name: Kathrin
  full_name: Tomasek, Kathrin
  id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
  last_name: Tomasek
  orcid: 0000-0003-3768-877X
- first_name: Enrique
  full_name: Balleza, Enrique
  last_name: Balleza
- first_name: Daniel
  full_name: Kiviet, Daniel
  last_name: Kiviet
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Gasper
  full_name: Tkacik, Gasper
  id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
  last_name: Tkacik
  orcid: 0000-0002-6699-1455
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multi-drug
    efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. 2017. doi:<a
    href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>
  apa: Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild,
    R., … Guet, C. C. (2017). Biased partitioning of the multi-drug efflux pump AcrAB-TolC
    underlies long-lived phenotypic heterogeneity. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/AT:ISTA:53">https://doi.org/10.15479/AT:ISTA:53</a>
  chicago: Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza,
    Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning
    of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity.”
    Institute of Science and Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:53">https://doi.org/10.15479/AT:ISTA:53</a>.
  ieee: T. Bergmiller <i>et al.</i>, “Biased partitioning of the multi-drug efflux
    pump AcrAB-TolC underlies long-lived phenotypic heterogeneity.” Institute of Science
    and Technology Austria, 2017.
  ista: Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik
    G, Guet CC. 2017. Biased partitioning of the multi-drug efflux pump AcrAB-TolC
    underlies long-lived phenotypic heterogeneity, Institute of Science and Technology
    Austria, <a href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>.
  mla: Bergmiller, Tobias, et al. <i>Biased Partitioning of the Multi-Drug Efflux
    Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity</i>. Institute of
    Science and Technology Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:53">10.15479/AT:ISTA:53</a>.
  short: T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild,
    G. Tkačik, C.C. Guet, (2017).
datarep_id: '53'
date_created: 2018-12-12T12:31:32Z
date_published: 2017-03-10T00:00:00Z
date_updated: 2025-09-11T07:05:03Z
day: '10'
ddc:
- '571'
department:
- _id: CaGu
- _id: GaTk
- _id: Bio
doi: 10.15479/AT:ISTA:53
file:
- access_level: open_access
  checksum: d77859af757ac8025c50c7b12b52eaf3
  content_type: application/zip
  creator: system
  date_created: 2018-12-12T13:02:38Z
  date_updated: 2020-07-14T12:47:03Z
  file_id: '5603'
  file_name: IST-2017-53-v1+1_Data_MDE.zip
  file_size: 6773204
  relation: main_file
file_date_updated: 2020-07-14T12:47:03Z
has_accepted_license: '1'
keyword:
- single cell microscopy
- mother machine microfluidic device
- AcrAB-TolC pump
- multi-drug efflux
- Escherichia coli
license: https://creativecommons.org/publicdomain/zero/1.0/
month: '03'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '665'
    relation: research_paper
    status: public
status: public
title: Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived
  phenotypic heterogeneity
tmp:
  image: /images/cc_0.png
  legal_code_url: https://creativecommons.org/publicdomain/zero/1.0/legalcode
  name: Creative Commons Public Domain Dedication (CC0 1.0)
  short: CC0 (1.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '5565'
abstract:
- lang: eng
  text: "One of the key questions in understanding plant development is how single
    cells behave in a larger context of the tissue. Therefore, it requires the observation
    of the whole organ with a high spatial- as well as temporal resolution over prolonged
    periods of time, which may cause photo-toxic effects. This protocol shows a plant
    sample preparation method for light-sheet microscopy, which is characterized by
    mounting the plant vertically on the surface of a gel. The plant is mounted in
    such a way that the roots are submerged in a liquid medium while the leaves remain
    in the air. In order to ensure photosynthetic activity of the plant, a custom-made
    lighting system illuminates the leaves. To keep the roots in darkness the water
    surface is covered with sheets of black plastic foil. This method allows long-term
    imaging of plant organ development in standardized conditions. \r\nThe Video is
    licensed under a CC BY NC ND license. "
acknowledgement: 'fund: FP7-ERC 0101109'
article_processing_charge: No
author:
- first_name: Daniel
  full_name: Von Wangenheim, Daniel
  id: 49E91952-F248-11E8-B48F-1D18A9856A87
  last_name: Von Wangenheim
  orcid: 0000-0002-6862-1247
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel. 2017. doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>
  apa: von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence
    microscopy of plant roots growing on the surface of a gel. Institute of Science
    and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a>
  chicago: Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet
    Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute
    of Science and Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a>.
  ieee: D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel.” Institute of Science and Technology
    Austria, 2017.
  ista: von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel, Institute of Science and Technology
    Austria, <a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>.
  mla: von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant
    Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology
    Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>.
  short: D. von Wangenheim, R. Hauschild, J. Friml, (2017).
datarep_id: '66'
date_created: 2018-12-12T12:31:34Z
date_published: 2017-04-10T00:00:00Z
date_updated: 2025-04-15T07:48:04Z
day: '10'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.15479/AT:ISTA:66
ec_funded: 1
file:
- access_level: open_access
  checksum: b7552fc23540a85dc5a22fd4484eae71
  content_type: video/mp4
  creator: system
  date_created: 2018-12-12T13:02:33Z
  date_updated: 2020-07-14T12:47:03Z
  file_id: '5599'
  file_name: IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4
  file_size: 101497758
  relation: main_file
file_date_updated: 2020-07-14T12:47:03Z
has_accepted_license: '1'
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publisher: Institute of Science and Technology Austria
publist_id: '6302'
related_material:
  record:
  - id: '1078'
    relation: research_paper
    status: public
status: public
title: Light Sheet Fluorescence microscopy of plant roots growing on the surface of
  a gel
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '5566'
abstract:
- lang: eng
  text: Current minimal version of TipTracker
article_processing_charge: No
author:
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
citation:
  ama: Hauschild R. Live tracking of moving samples in confocal microscopy for vertically
    grown roots. 2017. doi:<a href="https://doi.org/10.15479/AT:ISTA:69">10.15479/AT:ISTA:69</a>
  apa: Hauschild, R. (2017). Live tracking of moving samples in confocal microscopy
    for vertically grown roots. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:69">https://doi.org/10.15479/AT:ISTA:69</a>
  chicago: Hauschild, Robert. “Live Tracking of Moving Samples in Confocal Microscopy
    for Vertically Grown Roots.” Institute of Science and Technology Austria, 2017.
    <a href="https://doi.org/10.15479/AT:ISTA:69">https://doi.org/10.15479/AT:ISTA:69</a>.
  ieee: R. Hauschild, “Live tracking of moving samples in confocal microscopy for
    vertically grown roots.” Institute of Science and Technology Austria, 2017.
  ista: Hauschild R. 2017. Live tracking of moving samples in confocal microscopy
    for vertically grown roots, Institute of Science and Technology Austria, <a href="https://doi.org/10.15479/AT:ISTA:69">10.15479/AT:ISTA:69</a>.
  mla: Hauschild, Robert. <i>Live Tracking of Moving Samples in Confocal Microscopy
    for Vertically Grown Roots</i>. Institute of Science and Technology Austria, 2017,
    doi:<a href="https://doi.org/10.15479/AT:ISTA:69">10.15479/AT:ISTA:69</a>.
  short: R. Hauschild, (2017).
datarep_id: '69'
date_created: 2018-12-12T12:31:34Z
date_published: 2017-07-21T00:00:00Z
date_updated: 2025-04-15T07:48:05Z
day: '21'
ddc:
- '570'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:69
file:
- access_level: open_access
  checksum: a976000e6715106724a271cc9422be4a
  content_type: application/zip
  creator: system
  date_created: 2018-12-12T13:04:12Z
  date_updated: 2020-07-14T12:47:04Z
  file_id: '5636'
  file_name: IST-2017-69-v1+2_TipTrackerZeissLSM700.zip
  file_size: 1587986
  relation: main_file
file_date_updated: 2020-07-14T12:47:04Z
has_accepted_license: '1'
keyword:
- tool
- tracking
- confocal microscopy
license: https://creativecommons.org/licenses/by-sa/4.0/
month: '07'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '946'
    relation: research_paper
    status: public
status: public
title: Live tracking of moving samples in confocal microscopy for vertically grown
  roots
tmp:
  image: /images/cc_by_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
  name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
    BY-SA 4.0)
  short: CC BY-SA (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '5570'
abstract:
- lang: eng
  text: Matlab script to calculate the forward migration indexes (<d_y>/<L>) from
    TrackMate spot-statistics files.
article_processing_charge: No
author:
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
citation:
  ama: Hauschild R. Forward migration indexes. 2017. doi:<a href="https://doi.org/10.15479/AT:ISTA:75">10.15479/AT:ISTA:75</a>
  apa: Hauschild, R. (2017). Forward migration indexes. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/AT:ISTA:75">https://doi.org/10.15479/AT:ISTA:75</a>
  chicago: Hauschild, Robert. “Forward Migration Indexes.” Institute of Science and
    Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:75">https://doi.org/10.15479/AT:ISTA:75</a>.
  ieee: R. Hauschild, “Forward migration indexes.” Institute of Science and Technology
    Austria, 2017.
  ista: Hauschild R. 2017. Forward migration indexes, Institute of Science and Technology
    Austria, <a href="https://doi.org/10.15479/AT:ISTA:75">10.15479/AT:ISTA:75</a>.
  mla: Hauschild, Robert. <i>Forward Migration Indexes</i>. Institute of Science and
    Technology Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:75">10.15479/AT:ISTA:75</a>.
  short: R. Hauschild, (2017).
datarep_id: '75'
date_created: 2018-12-12T12:31:35Z
date_published: 2017-10-04T00:00:00Z
date_updated: 2024-02-21T13:47:14Z
day: '04'
ddc:
- '570'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:75
file:
- access_level: open_access
  checksum: cb7a2fa622460eca6231d659ce590e32
  content_type: application/octet-stream
  creator: system
  date_created: 2018-12-12T13:02:29Z
  date_updated: 2020-07-14T12:47:04Z
  file_id: '5596'
  file_name: IST-2017-75-v1+1_FMI.m
  file_size: 799
  relation: main_file
file_date_updated: 2020-07-14T12:47:04Z
has_accepted_license: '1'
keyword:
- Cell migration
- tracking
- forward migration index
- FMI
month: '10'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
status: public
title: Forward migration indexes
tmp:
  image: /images/cc_0.png
  legal_code_url: https://creativecommons.org/publicdomain/zero/1.0/legalcode
  name: Creative Commons Public Domain Dedication (CC0 1.0)
  short: CC0 (1.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '630'
abstract:
- lang: eng
  text: 'Background: Standards have become available to share semantically encoded
    vital parameters from medical devices, as required for example by personal healthcare
    records. Standardised sharing of biosignal data largely remains open. Objectives:
    The goal of this work is to explore available biosignal file format and data exchange
    standards and profiles, and to conceptualise end-To-end solutions. Methods: The
    authors reviewed and discussed available biosignal file format standards with
    other members of international standards development organisations (SDOs). Results:
    A raw concept for standards based acquisition, storage, archiving and sharing
    of biosignals was developed. The GDF format may serve for storing biosignals.
    Signals can then be shared using FHIR resources and may be stored on FHIR servers
    or in DICOM archives, with DICOM waveforms as one possible format. Conclusion:
    Currently a group of international SDOs (e.g. HL7, IHE, DICOM, IEEE) is engaged
    in intensive discussions. This discussion extends existing work that already was
    adopted by large implementer communities. The concept presented here only reports
    the current status of the discussion in Austria. The discussion will continue
    internationally, with results to be expected over the coming years.'
alternative_title:
- Studies in Health Technology and Informatics
article_processing_charge: No
author:
- first_name: Stefan
  full_name: Sauermann, Stefan
  last_name: Sauermann
- first_name: Veronika
  full_name: David, Veronika
  last_name: David
- first_name: Alois
  full_name: Schlögl, Alois
  id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
  last_name: Schlögl
  orcid: 0000-0002-5621-8100
- first_name: Reinhard
  full_name: Egelkraut, Reinhard
  last_name: Egelkraut
- first_name: Matthias
  full_name: Frohner, Matthias
  last_name: Frohner
- first_name: Birgit
  full_name: Pohn, Birgit
  last_name: Pohn
- first_name: Philipp
  full_name: Urbauer, Philipp
  last_name: Urbauer
- first_name: Alexander
  full_name: Mense, Alexander
  last_name: Mense
citation:
  ama: 'Sauermann S, David V, Schlögl A, et al. Biosignals standards and FHIR: The
    way to go. In: Vol 236. IOS Press; 2017:356-362. doi:<a href="https://doi.org/10.3233/978-1-61499-759-7-356">10.3233/978-1-61499-759-7-356</a>'
  apa: 'Sauermann, S., David, V., Schlögl, A., Egelkraut, R., Frohner, M., Pohn, B.,
    … Mense, A. (2017). Biosignals standards and FHIR: The way to go (Vol. 236, pp.
    356–362). Presented at the eHealth: Health Informatics Meets eHealth, Vienna,
    Austria: IOS Press. <a href="https://doi.org/10.3233/978-1-61499-759-7-356">https://doi.org/10.3233/978-1-61499-759-7-356</a>'
  chicago: 'Sauermann, Stefan, Veronika David, Alois Schlögl, Reinhard Egelkraut,
    Matthias Frohner, Birgit Pohn, Philipp Urbauer, and Alexander Mense. “Biosignals
    Standards and FHIR: The Way to Go,” 236:356–62. IOS Press, 2017. <a href="https://doi.org/10.3233/978-1-61499-759-7-356">https://doi.org/10.3233/978-1-61499-759-7-356</a>.'
  ieee: 'S. Sauermann <i>et al.</i>, “Biosignals standards and FHIR: The way to go,”
    presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria, 2017,
    vol. 236, pp. 356–362.'
  ista: 'Sauermann S, David V, Schlögl A, Egelkraut R, Frohner M, Pohn B, Urbauer
    P, Mense A. 2017. Biosignals standards and FHIR: The way to go. eHealth: Health
    Informatics Meets eHealth, Studies in Health Technology and Informatics, vol.
    236, 356–362.'
  mla: 'Sauermann, Stefan, et al. <i>Biosignals Standards and FHIR: The Way to Go</i>.
    Vol. 236, IOS Press, 2017, pp. 356–62, doi:<a href="https://doi.org/10.3233/978-1-61499-759-7-356">10.3233/978-1-61499-759-7-356</a>.'
  short: S. Sauermann, V. David, A. Schlögl, R. Egelkraut, M. Frohner, B. Pohn, P.
    Urbauer, A. Mense, in:, IOS Press, 2017, pp. 356–362.
conference:
  end_date: 2017-05-24
  location: Vienna, Austria
  name: 'eHealth: Health Informatics Meets eHealth'
  start_date: 2017-05-23
date_created: 2018-12-11T11:47:36Z
date_published: 2017-01-01T00:00:00Z
date_updated: 2025-09-11T07:27:53Z
day: '01'
ddc:
- '005'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.3233/978-1-61499-759-7-356
external_id:
  isi:
  - '000426828000047'
file:
- access_level: open_access
  checksum: 1254dcc5b04a996d97fad9a726b42727
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:11:56Z
  date_updated: 2020-07-14T12:47:27Z
  file_id: '4913'
  file_name: IST-2017-906-v1+1_SHTI236-0356.pdf
  file_size: 443635
  relation: main_file
file_date_updated: 2020-07-14T12:47:27Z
has_accepted_license: '1'
intvolume: '       236'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 356 - 362
publication_identifier:
  isbn:
  - 978-161499758-0
publication_status: published
publisher: IOS Press
publist_id: '7164'
pubrep_id: '906'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Biosignals standards and FHIR: The way to go'
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: conference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 236
year: '2017'
...
---
_id: '665'
abstract:
- lang: eng
  text: The molecular mechanisms underlying phenotypic variation in isogenic bacterial
    populations remain poorly understood.We report that AcrAB-TolC, the main multidrug
    efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell
    poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex
    formation. Mother cells inheriting old poles are phenotypically distinct and display
    increased drug efflux activity relative to daughters. Consequently, we find systematic
    and long-lived growth differences between mother and daughter cells in the presence
    of subinhibitory drug concentrations. A simple model for biased partitioning predicts
    a population structure of long-lived and highly heterogeneous phenotypes. This
    straightforward mechanism of generating sustained growth rate differences at subinhibitory
    antibiotic concentrations has implications for understanding the emergence of
    multidrug resistance in bacteria.
article_processing_charge: No
article_type: original
author:
- first_name: Tobias
  full_name: Bergmiller, Tobias
  id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
  last_name: Bergmiller
  orcid: 0000-0001-5396-4346
- first_name: Anna M
  full_name: Andersson, Anna M
  id: 2B8A40DA-F248-11E8-B48F-1D18A9856A87
  last_name: Andersson
  orcid: 0000-0003-2912-6769
- first_name: Kathrin
  full_name: Tomasek, Kathrin
  id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
  last_name: Tomasek
  orcid: 0000-0003-3768-877X
- first_name: Enrique
  full_name: Balleza, Enrique
  last_name: Balleza
- first_name: Daniel
  full_name: Kiviet, Daniel
  last_name: Kiviet
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Gasper
  full_name: Tkacik, Gasper
  id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
  last_name: Tkacik
  orcid: 0000-0002-6699-1455
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug
    efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. <i>Science</i>.
    2017;356(6335):311-315. doi:<a href="https://doi.org/10.1126/science.aaf4762">10.1126/science.aaf4762</a>
  apa: Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild,
    R., … Guet, C. C. (2017). Biased partitioning of the multidrug efflux pump AcrAB
    TolC underlies long lived phenotypic heterogeneity. <i>Science</i>. American Association
    for the Advancement of Science. <a href="https://doi.org/10.1126/science.aaf4762">https://doi.org/10.1126/science.aaf4762</a>
  chicago: Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza,
    Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning
    of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.”
    <i>Science</i>. American Association for the Advancement of Science, 2017. <a
    href="https://doi.org/10.1126/science.aaf4762">https://doi.org/10.1126/science.aaf4762</a>.
  ieee: T. Bergmiller <i>et al.</i>, “Biased partitioning of the multidrug efflux
    pump AcrAB TolC underlies long lived phenotypic heterogeneity,” <i>Science</i>,
    vol. 356, no. 6335. American Association for the Advancement of Science, pp. 311–315,
    2017.
  ista: Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik
    G, Guet CC. 2017. Biased partitioning of the multidrug efflux pump AcrAB TolC
    underlies long lived phenotypic heterogeneity. Science. 356(6335), 311–315.
  mla: Bergmiller, Tobias, et al. “Biased Partitioning of the Multidrug Efflux Pump
    AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” <i>Science</i>, vol.
    356, no. 6335, American Association for the Advancement of Science, 2017, pp.
    311–15, doi:<a href="https://doi.org/10.1126/science.aaf4762">10.1126/science.aaf4762</a>.
  short: T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild,
    G. Tkačik, C.C. Guet, Science 356 (2017) 311–315.
corr_author: '1'
date_created: 2018-12-11T11:47:48Z
date_published: 2017-04-21T00:00:00Z
date_updated: 2025-09-11T07:05:04Z
day: '21'
department:
- _id: CaGu
- _id: GaTk
- _id: Bio
doi: 10.1126/science.aaf4762
external_id:
  isi:
  - '000399540100060'
intvolume: '       356'
isi: 1
issue: '6335'
language:
- iso: eng
month: '04'
oa_version: None
page: 311 - 315
project:
- _id: 254E9036-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P28844-B27
  name: Biophysics of information processing in gene regulation
publication: Science
publication_identifier:
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '7064'
quality_controlled: '1'
related_material:
  record:
  - id: '5560'
    relation: popular_science
    status: public
scopus_import: '1'
status: public
title: Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long
  lived phenotypic heterogeneity
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 356
year: '2017'
...
---
_id: '672'
abstract:
- lang: eng
  text: Trafficking cells frequently transmigrate through epithelial and endothelial
    monolayers. How monolayers cooperate with the penetrating cells to support their
    transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic
    capillaries as a model system for transendothelial migration. We find that the
    chemokine CCL21, which is the decisive guidance cue for intravasation, mainly
    localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial
    cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes
    extracellularly enriched at the sites of endothelial cell-cell junctions. When
    we reconstitute the transmigration process in vitro, we find that secretion of
    CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and
    selective calcium chelation in lymphatic endothelium attenuates transmigration.
    Altogether, our data demonstrate a chemokine-mediated feedback between DCs and
    lymphatic endothelium, which facilitates transendothelial migration.
article_processing_charge: Yes
author:
- first_name: Kari
  full_name: Vaahtomeri, Kari
  id: 368EE576-F248-11E8-B48F-1D18A9856A87
  last_name: Vaahtomeri
  orcid: 0000-0001-7829-3518
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Matthias
  full_name: Mehling, Matthias
  id: 3C23B994-F248-11E8-B48F-1D18A9856A87
  last_name: Mehling
  orcid: 0000-0001-8599-1226
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Vaahtomeri K, Brown M, Hauschild R, et al. Locally triggered release of the
    chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia.
    <i>Cell Reports</i>. 2017;19(5):902-909. doi:<a href="https://doi.org/10.1016/j.celrep.2017.04.027">10.1016/j.celrep.2017.04.027</a>
  apa: Vaahtomeri, K., Brown, M., Hauschild, R., de Vries, I., Leithner, A. F., Mehling,
    M., … Sixt, M. K. (2017). Locally triggered release of the chemokine CCL21 promotes
    dendritic cell transmigration across lymphatic endothelia. <i>Cell Reports</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.celrep.2017.04.027">https://doi.org/10.1016/j.celrep.2017.04.027</a>
  chicago: Vaahtomeri, Kari, Markus Brown, Robert Hauschild, Ingrid de Vries, Alexander
    F Leithner, Matthias Mehling, Walter Kaufmann, and Michael K Sixt. “Locally Triggered
    Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic
    Endothelia.” <i>Cell Reports</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.celrep.2017.04.027">https://doi.org/10.1016/j.celrep.2017.04.027</a>.
  ieee: K. Vaahtomeri <i>et al.</i>, “Locally triggered release of the chemokine CCL21
    promotes dendritic cell transmigration across lymphatic endothelia,” <i>Cell Reports</i>,
    vol. 19, no. 5. Cell Press, pp. 902–909, 2017.
  ista: Vaahtomeri K, Brown M, Hauschild R, de Vries I, Leithner AF, Mehling M, Kaufmann
    W, Sixt MK. 2017. Locally triggered release of the chemokine CCL21 promotes dendritic
    cell transmigration across lymphatic endothelia. Cell Reports. 19(5), 902–909.
  mla: Vaahtomeri, Kari, et al. “Locally Triggered Release of the Chemokine CCL21
    Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” <i>Cell Reports</i>,
    vol. 19, no. 5, Cell Press, 2017, pp. 902–09, doi:<a href="https://doi.org/10.1016/j.celrep.2017.04.027">10.1016/j.celrep.2017.04.027</a>.
  short: K. Vaahtomeri, M. Brown, R. Hauschild, I. de Vries, A.F. Leithner, M. Mehling,
    W. Kaufmann, M.K. Sixt, Cell Reports 19 (2017) 902–909.
corr_author: '1'
date_created: 2018-12-11T11:47:50Z
date_published: 2017-05-02T00:00:00Z
date_updated: 2025-09-10T14:27:34Z
day: '02'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
- _id: EM-Fac
doi: 10.1016/j.celrep.2017.04.027
ec_funded: 1
external_id:
  isi:
  - '000402124100002'
file:
- access_level: open_access
  checksum: 8fdddaab1f1d76a6ec9ca94dcb6b07a2
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:14:54Z
  date_updated: 2020-07-14T12:47:38Z
  file_id: '5109'
  file_name: IST-2017-900-v1+1_1-s2.0-S2211124717305211-main.pdf
  file_size: 2248814
  relation: main_file
file_date_updated: 2020-07-14T12:47:38Z
has_accepted_license: '1'
intvolume: '        19'
isi: 1
issue: '5'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '05'
oa: 1
oa_version: Published Version
page: 902 - 909
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Cell Reports
publication_identifier:
  issn:
  - 2211-1247
publication_status: published
publisher: Cell Press
publist_id: '7052'
pubrep_id: '900'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration
  across lymphatic endothelia
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 19
year: '2017'
...
---
_id: '674'
abstract:
- lang: eng
  text: Navigation of cells along gradients of guidance cues is a determining step
    in many developmental and immunological processes. Gradients can either be soluble
    or immobilized to tissues as demonstrated for the haptotactic migration of dendritic
    cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate
    how gradient characteristics govern cellular response patterns, we here introduce
    an in vitro system allowing to track migratory responses of DCs to precisely controlled
    immobilized gradients of CCL21. We find that haptotactic sensing depends on the
    absolute CCL21 concentration and local steepness of the gradient, consistent with
    a scenario where DC directionality is governed by the signal-to-noise ratio of
    CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC
    guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore,
    we find that CCR7 signal termination by the G-protein-coupled receptor kinase
    6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient
    sensing in vitro and confirm those observations in vivo. These findings suggest
    that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal
    guidance in vivo.
article_processing_charge: No
author:
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Veronika
  full_name: Bierbaum, Veronika
  id: 3FD04378-F248-11E8-B48F-1D18A9856A87
  last_name: Bierbaum
- first_name: Kari
  full_name: Vaahtomeri, Kari
  id: 368EE576-F248-11E8-B48F-1D18A9856A87
  last_name: Vaahtomeri
  orcid: 0000-0001-7829-3518
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Anne
  full_name: Reversat, Anne
  id: 35B76592-F248-11E8-B48F-1D18A9856A87
  last_name: Reversat
  orcid: 0000-0003-0666-8928
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Teresa
  full_name: Tarrant, Teresa
  last_name: Tarrant
- first_name: Tobias
  full_name: Bollenbach, Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic
    chemokine gradients in a manner governed by signal to noise ratio and dependent
    on GRK6. <i>Current Biology</i>. 2017;27(9):1314-1325. doi:<a href="https://doi.org/10.1016/j.cub.2017.04.004">10.1016/j.cub.2017.04.004</a>
  apa: Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries,
    I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients
    in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current
    Biology</i>. Cell Press. <a href="https://doi.org/10.1016/j.cub.2017.04.004">https://doi.org/10.1016/j.cub.2017.04.004</a>
  chicago: Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus
    Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret
    Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio
    and Dependent on GRK6.” <i>Current Biology</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cub.2017.04.004">https://doi.org/10.1016/j.cub.2017.04.004</a>.
  ieee: J. Schwarz <i>et al.</i>, “Dendritic cells interpret haptotactic chemokine
    gradients in a manner governed by signal to noise ratio and dependent on GRK6,”
    <i>Current Biology</i>, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017.
  ista: Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner
    AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells
    interpret haptotactic chemokine gradients in a manner governed by signal to noise
    ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.
  mla: Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients
    in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current
    Biology</i>, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:<a href="https://doi.org/10.1016/j.cub.2017.04.004">10.1016/j.cub.2017.04.004</a>.
  short: J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries,
    A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt,
    Current Biology 27 (2017) 1314–1325.
corr_author: '1'
date_created: 2018-12-11T11:47:51Z
date_published: 2017-05-09T00:00:00Z
date_updated: 2025-09-10T14:26:47Z
day: '09'
department:
- _id: MiSi
- _id: Bio
- _id: NanoFab
doi: 10.1016/j.cub.2017.04.004
ec_funded: 1
external_id:
  isi:
  - '000400741700021'
intvolume: '        27'
isi: 1
issue: '9'
language:
- iso: eng
month: '05'
oa_version: None
page: 1314 - 1325
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Current Biology
publication_identifier:
  issn:
  - '09609822'
publication_status: published
publisher: Cell Press
publist_id: '7050'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dendritic cells interpret haptotactic chemokine gradients in a manner governed
  by signal to noise ratio and dependent on GRK6
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 27
year: '2017'
...
---
_id: '675'
abstract:
- lang: eng
  text: 'We report the enhancement of infrared absorption of chemisorbed carbon monoxide
    on platinum in the gap of plasmonic nanoantennas. Our method is based on the self-assembled
    formation of platinum nanoislands on nanoscopic dipole antenna arrays manufactured
    via electron beam lithography. We employ systematic variations of the plasmonic
    antenna resonance to precisely couple to the molecular stretch vibration of carbon
    monoxide adsorbed on the platinum nanoislands. Ultimately, we reach more than
    1500-fold infrared absorption enhancements, allowing for an ultrasensitive detection
    of a monolayer of chemisorbed carbon monoxide. The developed procedure can be
    adapted to other metal adsorbents and molecular species and could be utilized
    for coverage sensing in surface catalytic reactions. '
article_processing_charge: No
article_type: original
author:
- first_name: Johannes
  full_name: Haase, Johannes
  last_name: Haase
- first_name: Salvatore
  full_name: Bagiante, Salvatore
  id: 38ED402E-F248-11E8-B48F-1D18A9856A87
  last_name: Bagiante
  orcid: 0000-0002-0122-9603
- first_name: Hans
  full_name: Sigg, Hans
  last_name: Sigg
- first_name: Jeroen
  full_name: Van Bokhoven, Jeroen
  last_name: Van Bokhoven
citation:
  ama: Haase J, Bagiante S, Sigg H, Van Bokhoven J. Surface enhanced infrared absorption
    of chemisorbed carbon monoxide using plasmonic nanoantennas. <i>Optics Letters</i>.
    2017;42(10):1931-1934. doi:<a href="https://doi.org/10.1364/OL.42.001931">10.1364/OL.42.001931</a>
  apa: Haase, J., Bagiante, S., Sigg, H., &#38; Van Bokhoven, J. (2017). Surface enhanced
    infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas.
    <i>Optics Letters</i>. Optica Publishing Group. <a href="https://doi.org/10.1364/OL.42.001931">https://doi.org/10.1364/OL.42.001931</a>
  chicago: Haase, Johannes, Salvatore Bagiante, Hans Sigg, and Jeroen Van Bokhoven.
    “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic
    Nanoantennas.” <i>Optics Letters</i>. Optica Publishing Group, 2017. <a href="https://doi.org/10.1364/OL.42.001931">https://doi.org/10.1364/OL.42.001931</a>.
  ieee: J. Haase, S. Bagiante, H. Sigg, and J. Van Bokhoven, “Surface enhanced infrared
    absorption of chemisorbed carbon monoxide using plasmonic nanoantennas,” <i>Optics
    Letters</i>, vol. 42, no. 10. Optica Publishing Group, pp. 1931–1934, 2017.
  ista: Haase J, Bagiante S, Sigg H, Van Bokhoven J. 2017. Surface enhanced infrared
    absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics
    Letters. 42(10), 1931–1934.
  mla: Haase, Johannes, et al. “Surface Enhanced Infrared Absorption of Chemisorbed
    Carbon Monoxide Using Plasmonic Nanoantennas.” <i>Optics Letters</i>, vol. 42,
    no. 10, Optica Publishing Group, 2017, pp. 1931–34, doi:<a href="https://doi.org/10.1364/OL.42.001931">10.1364/OL.42.001931</a>.
  short: J. Haase, S. Bagiante, H. Sigg, J. Van Bokhoven, Optics Letters 42 (2017)
    1931–1934.
date_created: 2018-12-11T11:47:51Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2025-09-10T14:25:19Z
day: '15'
ddc:
- '530'
department:
- _id: NanoFab
doi: 10.1364/OL.42.001931
external_id:
  isi:
  - '000401424900016'
intvolume: '        42'
isi: 1
issue: '10'
language:
- iso: eng
month: '05'
oa_version: None
page: 1931 - 1934
publication: Optics Letters
publication_status: published
publisher: Optica Publishing Group
publist_id: '7048'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic
  nanoantennas
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 42
year: '2017'
...
---
_id: '693'
abstract:
- lang: eng
  text: 'Many central synapses contain a single presynaptic active zone and a single
    postsynaptic density. Vesicular release statistics at such “simple synapses” indicate
    that they contain a small complement of docking sites where vesicles repetitively
    dock and fuse. In this work, we investigate functional and morphological aspects
    of docking sites at simple synapses made between cerebellar parallel fibers and
    molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture
    replicas, we find that Cav2.1 channels form several clusters per active zone with
    about nine channels per cluster. The mean value and range of intersynaptic variation
    are similar for Cav2.1 cluster numbers and for functional estimates of docking-site
    numbers obtained from the maximum numbers of released vesicles per action potential.
    Both numbers grow in relation with synaptic size and decrease by a similar extent
    with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers
    were 3.15 at 2 wk (range: 1–10) and 2.03 at 4 wk (range: 1–4), whereas the mean
    numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1–8) and 2.37 at 4 wk (range:
    1–5). These changes were accompanied by decreases of miniature current amplitude
    (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2),
    and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic
    transmission with development. Altogether, these results suggest a close correspondence
    between the number of functionally defined vesicular docking sites and that of
    clusters of voltage-gated calcium channels. '
article_processing_charge: Yes (in subscription journal)
author:
- first_name: Takafumi
  full_name: Miki, Takafumi
  last_name: Miki
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Gerardo
  full_name: Malagon, Gerardo
  last_name: Malagon
- first_name: Laura
  full_name: Gomez, Laura
  last_name: Gomez
- first_name: Katsuhiko
  full_name: Tabuchi, Katsuhiko
  last_name: Tabuchi
- first_name: Masahiko
  full_name: Watanabe, Masahiko
  last_name: Watanabe
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Alain
  full_name: Marty, Alain
  last_name: Marty
citation:
  ama: Miki T, Kaufmann W, Malagon G, et al. Numbers of presynaptic Ca2+ channel clusters
    match those of functionally defined vesicular docking sites in single central
    synapses. <i>PNAS</i>. 2017;114(26):E5246-E5255. doi:<a href="https://doi.org/10.1073/pnas.1704470114">10.1073/pnas.1704470114</a>
  apa: Miki, T., Kaufmann, W., Malagon, G., Gomez, L., Tabuchi, K., Watanabe, M.,
    … Marty, A. (2017). Numbers of presynaptic Ca2+ channel clusters match those of
    functionally defined vesicular docking sites in single central synapses. <i>PNAS</i>.
    National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.1704470114">https://doi.org/10.1073/pnas.1704470114</a>
  chicago: Miki, Takafumi, Walter Kaufmann, Gerardo Malagon, Laura Gomez, Katsuhiko
    Tabuchi, Masahiko Watanabe, Ryuichi Shigemoto, and Alain Marty. “Numbers of Presynaptic
    Ca2+ Channel Clusters Match Those of Functionally Defined Vesicular Docking Sites
    in Single Central Synapses.” <i>PNAS</i>. National Academy of Sciences, 2017.
    <a href="https://doi.org/10.1073/pnas.1704470114">https://doi.org/10.1073/pnas.1704470114</a>.
  ieee: T. Miki <i>et al.</i>, “Numbers of presynaptic Ca2+ channel clusters match
    those of functionally defined vesicular docking sites in single central synapses,”
    <i>PNAS</i>, vol. 114, no. 26. National Academy of Sciences, pp. E5246–E5255,
    2017.
  ista: Miki T, Kaufmann W, Malagon G, Gomez L, Tabuchi K, Watanabe M, Shigemoto R,
    Marty A. 2017. Numbers of presynaptic Ca2+ channel clusters match those of functionally
    defined vesicular docking sites in single central synapses. PNAS. 114(26), E5246–E5255.
  mla: Miki, Takafumi, et al. “Numbers of Presynaptic Ca2+ Channel Clusters Match
    Those of Functionally Defined Vesicular Docking Sites in Single Central Synapses.”
    <i>PNAS</i>, vol. 114, no. 26, National Academy of Sciences, 2017, pp. E5246–55,
    doi:<a href="https://doi.org/10.1073/pnas.1704470114">10.1073/pnas.1704470114</a>.
  short: T. Miki, W. Kaufmann, G. Malagon, L. Gomez, K. Tabuchi, M. Watanabe, R. Shigemoto,
    A. Marty, PNAS 114 (2017) E5246–E5255.
corr_author: '1'
date_created: 2018-12-11T11:47:57Z
date_published: 2017-06-27T00:00:00Z
date_updated: 2025-09-10T14:00:03Z
day: '27'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: RySh
doi: 10.1073/pnas.1704470114
external_id:
  isi:
  - '000404108400028'
  pmid:
  - '28607047'
file:
- access_level: open_access
  checksum: 2ab75d554f3df4a34d20fa8040589b7e
  content_type: application/pdf
  creator: kschuh
  date_created: 2020-01-03T13:27:29Z
  date_updated: 2020-07-14T12:47:44Z
  file_id: '7223'
  file_name: 2017_PNAS_Miki.pdf
  file_size: 2721544
  relation: main_file
file_date_updated: 2020-07-14T12:47:44Z
has_accepted_license: '1'
intvolume: '       114'
isi: 1
issue: '26'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: E5246 - E5255
pmid: 1
publication: PNAS
publication_identifier:
  issn:
  - 0027-8424
publication_status: published
publisher: National Academy of Sciences
publist_id: '7013'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Numbers of presynaptic Ca2+ channel clusters match those of functionally defined
  vesicular docking sites in single central synapses
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 114
year: '2017'
...
---
_id: '727'
abstract:
- lang: eng
  text: 'Actin filaments polymerizing against membranes power endocytosis, vesicular
    traffic, and cell motility. In vitro reconstitution studies suggest that the structure
    and the dynamics of actin networks respond to mechanical forces. We demonstrate
    that lamellipodial actin of migrating cells responds to mechanical load when membrane
    tension is modulated. In a steady state, migrating cell filaments assume the canonical
    dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension
    triggers a dense network with a broadened range of angles, whereas decreased tension
    causes a shift to a sparse configuration dominated by filaments growing perpendicularly
    to the plasma membrane. We show that these responses emerge from the geometry
    of branched actin: when load per filament decreases, elongation speed increases
    and perpendicular filaments gradually outcompete others because they polymerize
    the shortest distance to the membrane, where they are protected from capping.
    This network-intrinsic geometrical adaptation mechanism tunes protrusive force
    in response to mechanical load.'
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: Jan
  full_name: Mueller, Jan
  last_name: Mueller
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Ingrid
  full_name: De Vries, Ingrid
  id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
  last_name: De Vries
- first_name: Arnon
  full_name: Lieber, Arnon
  last_name: Lieber
- first_name: Christoph
  full_name: Winkler, Christoph
  last_name: Winkler
- first_name: Karsten
  full_name: Kruse, Karsten
  last_name: Kruse
- first_name: John
  full_name: Small, John
  last_name: Small
- first_name: Christian
  full_name: Schmeiser, Christian
  last_name: Schmeiser
- first_name: Kinneret
  full_name: Keren, Kinneret
  last_name: Keren
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin
    networks. <i>Cell</i>. 2017;171(1):188-200. doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>
  apa: Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C.,
    … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. <i>Cell</i>.
    Cell Press. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>
  chicago: Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber,
    Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin
    Networks.” <i>Cell</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cell.2017.07.051">https://doi.org/10.1016/j.cell.2017.07.051</a>.
  ieee: J. Mueller <i>et al.</i>, “Load adaptation of lamellipodial actin networks,”
    <i>Cell</i>, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.
  ista: Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K,
    Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of
    lamellipodial actin networks. Cell. 171(1), 188–200.
  mla: Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” <i>Cell</i>,
    vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:<a href="https://doi.org/10.1016/j.cell.2017.07.051">10.1016/j.cell.2017.07.051</a>.
  short: J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K.
    Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017)
    188–200.
corr_author: '1'
date_created: 2018-12-11T11:48:10Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2025-07-10T11:54:27Z
day: '21'
department:
- _id: MiSi
- _id: Bio
doi: 10.1016/j.cell.2017.07.051
ec_funded: 1
external_id:
  isi:
  - '000411331800020'
intvolume: '       171'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
page: 188 - 200
project:
- _id: 25AD6156-B435-11E9-9278-68D0E5697425
  grant_number: LS13-029
  name: Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Cell
publication_identifier:
  issn:
  - 0092-8674
publication_status: published
publisher: Cell Press
publist_id: '6951'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Load adaptation of lamellipodial actin networks
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 171
year: '2017'
...
---
_id: '988'
abstract:
- lang: eng
  text: The current-phase relation (CPR) of a Josephson junction (JJ) determines how
    the supercurrent evolves with the superconducting phase difference across the
    junction. Knowledge of the CPR is essential in order to understand the response
    of a JJ to various external parameters. Despite the rising interest in ultraclean
    encapsulated graphene JJs, the CPR of such junctions remains unknown. Here, we
    use a fully gate-tunable graphene superconducting quantum intereference device
    (SQUID) to determine the CPR of ballistic graphene JJs. Each of the two JJs in
    the SQUID is made with graphene encapsulated in hexagonal boron nitride. By independently
    controlling the critical current of the JJs, we can operate the SQUID either in
    a symmetric or asymmetric configuration. The highly asymmetric SQUID allows us
    to phase-bias one of the JJs and thereby directly obtain its CPR. The CPR is found
    to be skewed, deviating significantly from a sinusoidal form. The skewness can
    be tuned with the gate voltage and oscillates in antiphase with Fabry-Pérot resistance
    oscillations of the ballistic graphene cavity. We compare our experiments with
    tight-binding calculations that include realistic graphene-superconductor interfaces
    and find a good qualitative agreement.
article_processing_charge: No
author:
- first_name: Gaurav
  full_name: Nanda, Gaurav
  last_name: Nanda
- first_name: Juan L
  full_name: Aguilera Servin, Juan L
  id: 2A67C376-F248-11E8-B48F-1D18A9856A87
  last_name: Aguilera Servin
  orcid: 0000-0002-2862-8372
- first_name: Péter
  full_name: Rakyta, Péter
  last_name: Rakyta
- first_name: Andor
  full_name: Kormányos, Andor
  last_name: Kormányos
- first_name: Reinhold
  full_name: Kleiner, Reinhold
  last_name: Kleiner
- first_name: Dieter
  full_name: Koelle, Dieter
  last_name: Koelle
- first_name: Kazuo
  full_name: Watanabe, Kazuo
  last_name: Watanabe
- first_name: Takashi
  full_name: Taniguchi, Takashi
  last_name: Taniguchi
- first_name: Lieven
  full_name: Vandersypen, Lieven
  last_name: Vandersypen
- first_name: Srijit
  full_name: Goswami, Srijit
  last_name: Goswami
citation:
  ama: Nanda G, Aguilera Servin JL, Rakyta P, et al. Current-phase relation of ballistic
    graphene Josephson junctions. <i>Nano Letters</i>. 2017;17(6):3396-3401. doi:<a
    href="https://doi.org/10.1021/acs.nanolett.7b00097">10.1021/acs.nanolett.7b00097</a>
  apa: Nanda, G., Aguilera Servin, J. L., Rakyta, P., Kormányos, A., Kleiner, R.,
    Koelle, D., … Goswami, S. (2017). Current-phase relation of ballistic graphene
    Josephson junctions. <i>Nano Letters</i>. American Chemical Society. <a href="https://doi.org/10.1021/acs.nanolett.7b00097">https://doi.org/10.1021/acs.nanolett.7b00097</a>
  chicago: Nanda, Gaurav, Juan L Aguilera Servin, Péter Rakyta, Andor Kormányos, Reinhold
    Kleiner, Dieter Koelle, Kazuo Watanabe, Takashi Taniguchi, Lieven Vandersypen,
    and Srijit Goswami. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.”
    <i>Nano Letters</i>. American Chemical Society, 2017. <a href="https://doi.org/10.1021/acs.nanolett.7b00097">https://doi.org/10.1021/acs.nanolett.7b00097</a>.
  ieee: G. Nanda <i>et al.</i>, “Current-phase relation of ballistic graphene Josephson
    junctions,” <i>Nano Letters</i>, vol. 17, no. 6. American Chemical Society, pp.
    3396–3401, 2017.
  ista: Nanda G, Aguilera Servin JL, Rakyta P, Kormányos A, Kleiner R, Koelle D, Watanabe
    K, Taniguchi T, Vandersypen L, Goswami S. 2017. Current-phase relation of ballistic
    graphene Josephson junctions. Nano Letters. 17(6), 3396–3401.
  mla: Nanda, Gaurav, et al. “Current-Phase Relation of Ballistic Graphene Josephson
    Junctions.” <i>Nano Letters</i>, vol. 17, no. 6, American Chemical Society, 2017,
    pp. 3396–401, doi:<a href="https://doi.org/10.1021/acs.nanolett.7b00097">10.1021/acs.nanolett.7b00097</a>.
  short: G. Nanda, J.L. Aguilera Servin, P. Rakyta, A. Kormányos, R. Kleiner, D. Koelle,
    K. Watanabe, T. Taniguchi, L. Vandersypen, S. Goswami, Nano Letters 17 (2017)
    3396–3401.
date_created: 2018-12-11T11:49:33Z
date_published: 2017-05-05T00:00:00Z
date_updated: 2025-07-10T12:02:04Z
day: '05'
ddc:
- '621'
department:
- _id: NanoFab
doi: 10.1021/acs.nanolett.7b00097
external_id:
  isi:
  - '000403631600011'
file:
- access_level: open_access
  checksum: 22021daa90cf13b01becd776838acb7b
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:50Z
  date_updated: 2020-07-14T12:48:18Z
  file_id: '5037'
  file_name: IST-2017-826-v1+1_2017_Aguilera-Servin_Current.pdf
  file_size: 508638
  relation: main_file
file_date_updated: 2020-07-14T12:48:18Z
has_accepted_license: '1'
intvolume: '        17'
isi: 1
issue: '6'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 3396 - 3401
publication: Nano Letters
publication_identifier:
  issn:
  - 1530-6984
publication_status: published
publisher: American Chemical Society
publist_id: '6412'
pubrep_id: '826'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Current-phase relation of ballistic graphene Josephson junctions
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2017'
...
---
_id: '676'
abstract:
- lang: eng
  text: The segregation of different cell types into distinct tissues is a fundamental
    process in metazoan development. Differences in cell adhesion and cortex tension
    are commonly thought to drive cell sorting by regulating tissue surface tension
    (TST). However, the role that differential TST plays in cell segregation within
    the developing embryo is as yet unclear. Here, we have analyzed the role of differential
    TST for germ layer progenitor cell segregation during zebrafish gastrulation.
    Contrary to previous observations that differential TST drives germ layer progenitor
    cell segregation in vitro, we show that germ layers display indistinguishable
    TST within the gastrulating embryo, arguing against differential TST driving germ
    layer progenitor cell segregation in vivo. We further show that the osmolarity
    of the interstitial fluid (IF) is an important factor that influences germ layer
    TST in vivo, and that lower osmolarity of the IF compared with standard cell culture
    medium can explain why germ layers display differential TST in culture but not
    in vivo. Finally, we show that directed migration of mesendoderm progenitors is
    required for germ layer progenitor cell segregation and germ layer formation.
article_processing_charge: No
article_type: original
author:
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Jim
  full_name: Veldhuis, Jim
  last_name: Veldhuis
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Jean-Léon
  full_name: Maître, Jean-Léon
  id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
  last_name: Maître
  orcid: 0000-0002-3688-1474
- first_name: Wayne
  full_name: Brodland, Wayne
  last_name: Brodland
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates
    the action of differential tissue surface tension in progenitor cell segregation
    during gastrulation. <i>Development</i>. 2017;144(10):1798-1806. doi:<a href="https://doi.org/10.1242/dev.144964">10.1242/dev.144964</a>
  apa: Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W.,
    &#38; Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the
    action of differential tissue surface tension in progenitor cell segregation during
    gastrulation. <i>Development</i>. Company of Biologists. <a href="https://doi.org/10.1242/dev.144964">https://doi.org/10.1242/dev.144964</a>
  chicago: Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître,
    Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity
    Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell
    Segregation during Gastrulation.” <i>Development</i>. Company of Biologists, 2017.
    <a href="https://doi.org/10.1242/dev.144964">https://doi.org/10.1242/dev.144964</a>.
  ieee: G. Krens <i>et al.</i>, “Interstitial fluid osmolarity modulates the action
    of differential tissue surface tension in progenitor cell segregation during gastrulation,”
    <i>Development</i>, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.
  ista: Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg
    C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential
    tissue surface tension in progenitor cell segregation during gastrulation. Development.
    144(10), 1798–1806.
  mla: Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action
    of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.”
    <i>Development</i>, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806,
    doi:<a href="https://doi.org/10.1242/dev.144964">10.1242/dev.144964</a>.
  short: G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J.
    Heisenberg, Development 144 (2017) 1798–1806.
corr_author: '1'
date_created: 2018-12-11T11:47:52Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2026-04-28T22:31:01Z
day: '15'
ddc:
- '570'
department:
- _id: Bio
- _id: CaHe
doi: 10.1242/dev.144964
external_id:
  isi:
  - '000402275900007'
  pmid:
  - '28512197'
file:
- access_level: open_access
  checksum: bc25125fb664706cdf180e061429f91d
  content_type: application/pdf
  creator: dernst
  date_created: 2019-09-24T06:56:22Z
  date_updated: 2020-07-14T12:47:39Z
  file_id: '6905'
  file_name: 2017_Development_Krens.pdf
  file_size: 8194516
  relation: main_file
file_date_updated: 2020-07-14T12:47:39Z
has_accepted_license: '1'
intvolume: '       144'
isi: 1
issue: '10'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1798 - 1806
pmid: 1
publication: Development
publication_identifier:
  issn:
  - 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '7047'
quality_controlled: '1'
related_material:
  record:
  - id: '961'
    relation: dissertation_contains
    status: public
  - id: '50'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Interstitial fluid osmolarity modulates the action of differential tissue surface
  tension in progenitor cell segregation during gastrulation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 144
year: '2017'
...
---
_id: '661'
abstract:
- lang: eng
  text: During embryonic development, mechanical forces are essential for cellular
    rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish
    embryo, friction forces are generated at the interface between anterior axial
    mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole
    and neurectoderm progenitors moving in the opposite direction towards the vegetal
    pole of the embryo. These friction forces lead to global rearrangement of cells
    within the neurectoderm and determine the position of the neural anlage. Using
    a combination of experiments and simulations, we show that this process depends
    on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated
    adhesion between those tissues. Our data thus establish the emergence of friction
    forces at the interface between moving tissues as a critical force-generating
    process shaping the embryo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
author:
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Zsuzsa
  full_name: Ákos, Zsuzsa
  last_name: Ákos
- first_name: Silvia
  full_name: Grigolon, Silvia
  last_name: Grigolon
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Verena
  full_name: Ruprecht, Verena
  last_name: Ruprecht
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Masazumi
  full_name: Tada, Masazumi
  last_name: Tada
- first_name: Björn
  full_name: Hof, Björn
  id: 3A374330-F248-11E8-B48F-1D18A9856A87
  last_name: Hof
  orcid: 0000-0003-2057-2754
- first_name: Tamás
  full_name: Vicsek, Tamás
  last_name: Vicsek
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage.
    <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>
  apa: Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D.,
    … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature
    Cell Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>
  chicago: Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena
    Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural
    Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>.
  ieee: M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature
    Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.
  ista: Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M,
    Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction
    forces position the neural anlage. Nature Cell Biology. 19, 306–317.
  mla: Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature
    Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>.
  short: M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M.
    Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg,
    Nature Cell Biology 19 (2017) 306–317.
corr_author: '1'
date_created: 2018-12-11T11:47:46Z
date_published: 2017-03-27T00:00:00Z
date_updated: 2026-04-28T22:31:01Z
day: '27'
department:
- _id: CaHe
- _id: BjHo
- _id: Bio
doi: 10.1038/ncb3492
ec_funded: 1
external_id:
  isi:
  - '000397917000009'
  pmid:
  - '28346437'
intvolume: '        19'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://europepmc.org/articles/pmc5635970
month: '03'
oa: 1
oa_version: Submitted Version
page: 306 - 317
pmid: 1
project:
- _id: 25152F3A-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '306589'
  name: Decoding the complexity of turbulence at its origin
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_identifier:
  issn:
  - 1465-7392
publication_status: published
publisher: Nature Publishing Group
publist_id: '7074'
quality_controlled: '1'
related_material:
  record:
  - id: '8350'
    relation: dissertation_contains
    status: public
  - id: '50'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Friction forces position the neural anlage
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 19
year: '2017'
...
---
_id: '1154'
abstract:
- lang: eng
  text: "Cellular locomotion is a central hallmark of eukaryotic life. It is governed
    by cell-extrinsic molecular factors, which can either emerge in the soluble phase
    or as immobilized, often adhesive ligands. To encode for direction, every cue
    must be present as a spatial or temporal gradient. Here, we developed a microfluidic
    chamber that allows measurement of cell migration in combined response to surface
    immobilized and soluble molecular gradients. As a proof of principle we study
    the response of dendritic cells to their major guidance cues, chemokines. The
    majority of data on chemokine gradient sensing is based on in vitro studies employing
    soluble gradients. Despite evidence suggesting that in vivo chemokines are often
    immobilized to sugar residues, limited information is available how cells respond
    to immobilized chemokines. We tracked migration of dendritic cells towards immobilized
    gradients of the chemokine CCL21 and varying superimposed soluble gradients of
    CCL19. Differential migratory patterns illustrate the potential of our setup to
    quantitatively study the competitive response to both types of gradients. Beyond
    chemokines our approach is broadly applicable to alternative systems of chemo-
    and haptotaxis such as cells migrating along gradients of adhesion receptor ligands
    vs. any soluble cue. \r\n"
acknowledgement: 'This work was supported by the Swiss National Science Foundation
  (Ambizione fellowship; PZ00P3-154733 to M.M.), the Swiss Multiple Sclerosis Society
  (research support to M.M.), a fellowship from the Boehringer Ingelheim Fonds (BIF)
  to J.S., the European Research Council (grant ERC GA 281556) and a START award from
  the Austrian Science Foundation (FWF) to M.S. #BioimagingFacility'
article_number: '36440'
article_processing_charge: No
author:
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Veronika
  full_name: Bierbaum, Veronika
  id: 3FD04378-F248-11E8-B48F-1D18A9856A87
  last_name: Bierbaum
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Tino
  full_name: Frank, Tino
  last_name: Frank
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Mark Tobias
  full_name: Bollenbach, Mark Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
- first_name: Savaş
  full_name: Tay, Savaş
  last_name: Tay
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Matthias
  full_name: Mehling, Matthias
  id: 3C23B994-F248-11E8-B48F-1D18A9856A87
  last_name: Mehling
  orcid: 0000-0001-8599-1226
citation:
  ama: Schwarz J, Bierbaum V, Merrin J, et al. A microfluidic device for measuring
    cell migration towards substrate bound and soluble chemokine gradients. <i>Scientific
    Reports</i>. 2016;6. doi:<a href="https://doi.org/10.1038/srep36440">10.1038/srep36440</a>
  apa: Schwarz, J., Bierbaum, V., Merrin, J., Frank, T., Hauschild, R., Bollenbach,
    M. T., … Mehling, M. (2016). A microfluidic device for measuring cell migration
    towards substrate bound and soluble chemokine gradients. <i>Scientific Reports</i>.
    Nature Publishing Group. <a href="https://doi.org/10.1038/srep36440">https://doi.org/10.1038/srep36440</a>
  chicago: Schwarz, Jan, Veronika Bierbaum, Jack Merrin, Tino Frank, Robert Hauschild,
    Mark Tobias Bollenbach, Savaş Tay, Michael K Sixt, and Matthias Mehling. “A Microfluidic
    Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine
    Gradients.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href="https://doi.org/10.1038/srep36440">https://doi.org/10.1038/srep36440</a>.
  ieee: J. Schwarz <i>et al.</i>, “A microfluidic device for measuring cell migration
    towards substrate bound and soluble chemokine gradients,” <i>Scientific Reports</i>,
    vol. 6. Nature Publishing Group, 2016.
  ista: Schwarz J, Bierbaum V, Merrin J, Frank T, Hauschild R, Bollenbach MT, Tay
    S, Sixt MK, Mehling M. 2016. A microfluidic device for measuring cell migration
    towards substrate bound and soluble chemokine gradients. Scientific Reports. 6,
    36440.
  mla: Schwarz, Jan, et al. “A Microfluidic Device for Measuring Cell Migration towards
    Substrate Bound and Soluble Chemokine Gradients.” <i>Scientific Reports</i>, vol.
    6, 36440, Nature Publishing Group, 2016, doi:<a href="https://doi.org/10.1038/srep36440">10.1038/srep36440</a>.
  short: J. Schwarz, V. Bierbaum, J. Merrin, T. Frank, R. Hauschild, M.T. Bollenbach,
    S. Tay, M.K. Sixt, M. Mehling, Scientific Reports 6 (2016).
date_created: 2018-12-11T11:50:27Z
date_published: 2016-11-07T00:00:00Z
date_updated: 2025-09-22T09:56:13Z
day: '07'
ddc:
- '579'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
- _id: ToBo
doi: 10.1038/srep36440
ec_funded: 1
external_id:
  isi:
  - '000387118300001'
file:
- access_level: open_access
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:09:32Z
  date_updated: 2018-12-12T10:09:32Z
  file_id: '4756'
  file_name: IST-2017-744-v1+1_srep36440.pdf
  file_size: 2353456
  relation: main_file
file_date_updated: 2018-12-12T10:09:32Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '6204'
pubrep_id: '744'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A microfluidic device for measuring cell migration towards substrate bound
  and soluble chemokine gradients
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 6
year: '2016'
...
---
_id: '1350'
abstract:
- lang: eng
  text: "The hippocampal CA3 region plays a key role in learning and memory. Recurrent
    CA3–CA3\r\nsynapses are thought to be the subcellular substrate of pattern completion.
    However, the\r\nsynaptic mechanisms of this network computation remain enigmatic.
    To investigate these mechanisms, we combined functional connectivity analysis
    with network modeling.\r\nSimultaneous recording fromup to eight CA3 pyramidal
    neurons revealed that connectivity was sparse, spatially uniform, and highly enriched
    in disynaptic motifs (reciprocal, convergence,divergence, and chain motifs). Unitary
    connections were composed of one or two synaptic contacts, suggesting efficient
    use of postsynaptic space. Real-size modeling indicated that CA3 networks with
    sparse connectivity, disynaptic motifs, and single-contact connections robustly
    generated pattern completion.Thus, macro- and microconnectivity contribute to
    efficient\r\nmemory storage and retrieval in hippocampal networks."
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: José
  full_name: Guzmán, José
  id: 30CC5506-F248-11E8-B48F-1D18A9856A87
  last_name: Guzmán
  orcid: 0000-0003-2209-5242
- first_name: Alois
  full_name: Schlögl, Alois
  id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
  last_name: Schlögl
  orcid: 0000-0002-5621-8100
- first_name: Michael
  full_name: Frotscher, Michael
  last_name: Frotscher
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Guzmán J, Schlögl A, Frotscher M, Jonas PM. Synaptic mechanisms of pattern
    completion in the hippocampal CA3 network. <i>Science</i>. 2016;353(6304):1117-1123.
    doi:<a href="https://doi.org/10.1126/science.aaf1836">10.1126/science.aaf1836</a>
  apa: Guzmán, J., Schlögl, A., Frotscher, M., &#38; Jonas, P. M. (2016). Synaptic
    mechanisms of pattern completion in the hippocampal CA3 network. <i>Science</i>.
    American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.aaf1836">https://doi.org/10.1126/science.aaf1836</a>
  chicago: Guzmán, José, Alois Schlögl, Michael Frotscher, and Peter M Jonas. “Synaptic
    Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” <i>Science</i>.
    American Association for the Advancement of Science, 2016. <a href="https://doi.org/10.1126/science.aaf1836">https://doi.org/10.1126/science.aaf1836</a>.
  ieee: J. Guzmán, A. Schlögl, M. Frotscher, and P. M. Jonas, “Synaptic mechanisms
    of pattern completion in the hippocampal CA3 network,” <i>Science</i>, vol. 353,
    no. 6304. American Association for the Advancement of Science, pp. 1117–1123,
    2016.
  ista: Guzmán J, Schlögl A, Frotscher M, Jonas PM. 2016. Synaptic mechanisms of pattern
    completion in the hippocampal CA3 network. Science. 353(6304), 1117–1123.
  mla: Guzmán, José, et al. “Synaptic Mechanisms of Pattern Completion in the Hippocampal
    CA3 Network.” <i>Science</i>, vol. 353, no. 6304, American Association for the
    Advancement of Science, 2016, pp. 1117–23, doi:<a href="https://doi.org/10.1126/science.aaf1836">10.1126/science.aaf1836</a>.
  short: J. Guzmán, A. Schlögl, M. Frotscher, P.M. Jonas, Science 353 (2016) 1117–1123.
corr_author: '1'
date_created: 2018-12-11T11:51:31Z
date_published: 2016-09-09T00:00:00Z
date_updated: 2025-09-22T08:12:10Z
day: '09'
ddc:
- '570'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.1126/science.aaf1836
ec_funded: 1
external_id:
  isi:
  - '000382626800045'
file:
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  checksum: 89caefa4e181424cbf0aecc835fcc5ec
  content_type: application/pdf
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  date_created: 2018-12-12T10:12:27Z
  date_updated: 2020-07-14T12:44:46Z
  file_id: '4945'
  file_name: IST-2017-823-v1+1_aaf1836_CombinedPDF_v2-1.pdf
  file_size: 19408143
  relation: main_file
file_date_updated: 2020-07-14T12:44:46Z
has_accepted_license: '1'
intvolume: '       353'
isi: 1
issue: '6304'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Preprint
page: 1117 - 1123
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '268548'
  name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P24909-B24
  name: Mechanisms of transmitter release at GABAergic synapses
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5899'
pubrep_id: '823'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synaptic mechanisms of pattern completion in the hippocampal CA3 network
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 353
year: '2016'
...
---
_id: '12903'
article_processing_charge: No
author:
- first_name: Alois
  full_name: Schlögl, Alois
  id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
  last_name: Schlögl
  orcid: 0000-0002-5621-8100
- first_name: Stephan
  full_name: Stadlbauer, Stephan
  id: 4D0BC184-F248-11E8-B48F-1D18A9856A87
  last_name: Stadlbauer
citation:
  ama: 'Schlögl A, Stadlbauer S. High performance computing at IST Austria: Modelling
    the human hippocampus. In: <i>AHPC16 - Austrian HPC Meeting 2016</i>. VSC - Vienna
    Scientific Cluster; 2016:37.'
  apa: 'Schlögl, A., &#38; Stadlbauer, S. (2016). High performance computing at IST
    Austria: Modelling the human hippocampus. In <i>AHPC16 - Austrian HPC Meeting
    2016</i> (p. 37). Grundlsee, Austria: VSC - Vienna Scientific Cluster.'
  chicago: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at
    IST Austria: Modelling the Human Hippocampus.” In <i>AHPC16 - Austrian HPC Meeting
    2016</i>, 37. VSC - Vienna Scientific Cluster, 2016.'
  ieee: 'A. Schlögl and S. Stadlbauer, “High performance computing at IST Austria:
    Modelling the human hippocampus,” in <i>AHPC16 - Austrian HPC Meeting 2016</i>,
    Grundlsee, Austria, 2016, p. 37.'
  ista: 'Schlögl A, Stadlbauer S. 2016. High performance computing at IST Austria:
    Modelling the human hippocampus. AHPC16 - Austrian HPC Meeting 2016. AHPC: Austrian
    HPC Meeting, 37.'
  mla: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST
    Austria: Modelling the Human Hippocampus.” <i>AHPC16 - Austrian HPC Meeting 2016</i>,
    VSC - Vienna Scientific Cluster, 2016, p. 37.'
  short: A. Schlögl, S. Stadlbauer, in:, AHPC16 - Austrian HPC Meeting 2016, VSC -
    Vienna Scientific Cluster, 2016, p. 37.
conference:
  end_date: 2016-02-24
  location: Grundlsee, Austria
  name: 'AHPC: Austrian HPC Meeting'
  start_date: 2016-02-22
corr_author: '1'
date_created: 2023-05-05T12:54:47Z
date_published: 2016-02-24T00:00:00Z
date_updated: 2024-10-09T21:05:23Z
day: '24'
ddc:
- '000'
department:
- _id: ScienComp
- _id: PeJo
file:
- access_level: open_access
  checksum: 4a7b00362e81358d568f5e216fa03c3e
  content_type: application/pdf
  creator: dernst
  date_created: 2023-05-16T07:03:56Z
  date_updated: 2023-05-16T07:03:56Z
  file_id: '12968'
  file_name: 2016_AHPC_Schloegl.pdf
  file_size: 1073523
  relation: main_file
  success: 1
file_date_updated: 2023-05-16T07:03:56Z
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc16/BOOKLET_AHPC16.pdf
month: '02'
oa: 1
oa_version: Published Version
page: '37'
publication: AHPC16 - Austrian HPC Meeting 2016
publication_status: published
publisher: VSC - Vienna Scientific Cluster
quality_controlled: '1'
status: public
title: 'High performance computing at IST Austria: Modelling the human hippocampus'
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2016'
...
