---
OA_place: publisher
OA_type: hybrid
_id: '14257'
abstract:
- lang: eng
text: Mapping the complex and dense arrangement of cells and their connectivity
in brain tissue demands nanoscale spatial resolution imaging. Super-resolution
optical microscopy excels at visualizing specific molecules and individual cells
but fails to provide tissue context. Here we developed Comprehensive Analysis
of Tissues across Scales (CATS), a technology to densely map brain tissue architecture
from millimeter regional to nanometer synaptic scales in diverse chemically fixed
brain preparations, including rodent and human. CATS uses fixation-compatible
extracellular labeling and optical imaging, including stimulated emission depletion
or expansion microscopy, to comprehensively delineate cellular structures. It
enables three-dimensional reconstruction of single synapses and mapping of synaptic
connectivity by identification and analysis of putative synaptic cleft regions.
Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed
and quantified the synaptic input and output structure of identified neurons.
We furthermore demonstrate applicability to clinically derived human tissue samples,
including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing
the cellular architecture of brain tissue in health and disease.
acknowledged_ssus:
- _id: ScienComp
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
- _id: E-Lib
acknowledgement: 'We thank J. Vorlaufer, N. Agudelo-Dueñas, W. Jahr and A. Wartak
for microscope maintenance and troubleshooting; C. Kreuzinger, A. Freeman and I.
Erber for technical assistance; and M. Tomschik for support with obtaining human
samples. We gratefully acknowledge E. Miguel for setting up webKnossos and M. Šuplata
for computational support and hardware control. We are grateful to R. Shigemoto
and B. Bickel for generous support and M. Sixt and S. Boyd (Stanford University)
for discussions and critical reading of the paper. PSD95-HaloTag mice were kindly
provided by S. Grant (University of Edinburgh). We acknowledge expert support by
Institute of Science and Technology Austria’s scientific computing, imaging and
optics, preclinical and lab support facilities and by the Miba machine shop and
library. We gratefully acknowledge funding by the following sources: Austrian Science
Fund (FWF) grant I3600-B27 (J.G.D.); Austrian Science Fund (FWF) grant DK W1232
(J.G.D. and J.M.M.); Austrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award
(P.J.); Austrian Science Fund (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27
(R.H.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.);
European Union’s Horizon 2020 research and innovation programme, European Research
Council (ERC) grant 715508 – REVERSEAUTISM (G.N.); European Union’s Horizon 2020
research and innovation programme, European Research Council (ERC) grant 692692
– GIANTSYN (P.J.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under
the EU Horizon 2020 program (J.M.M. and J.L.); and Marie Skłodowska-Curie Actions
Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.).'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Hana
full_name: Korinkova, Hana
id: ee3cb6ca-ec98-11ea-ae11-ff703e2254ed
last_name: Korinkova
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Alessandro
full_name: Venturino, Alessandro
id: 41CB84B2-F248-11E8-B48F-1D18A9856A87
last_name: Venturino
orcid: 0000-0003-2356-9403
- first_name: Karl
full_name: Roessler, Karl
last_name: Roessler
- first_name: Thomas
full_name: Czech, Thomas
last_name: Czech
- first_name: Romana
full_name: Höftberger, Romana
last_name: Höftberger
- first_name: Sandra
full_name: Siegert, Sandra
id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
last_name: Siegert
orcid: 0000-0001-8635-0877
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Michalska JM, Lyudchik J, Velicky P, et al. Imaging brain tissue architecture
across millimeter to nanometer scales. Nature Biotechnology. 2024;42:1051-1064.
doi:10.1038/s41587-023-01911-8
apa: Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri,
A., … Danzl, J. G. (2024). Imaging brain tissue architecture across millimeter
to nanometer scales. Nature Biotechnology. Springer Nature. https://doi.org/10.1038/s41587-023-01911-8
chicago: Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake
Watson, Alban Cenameri, Christoph M Sommer, et al. “Imaging Brain Tissue Architecture
across Millimeter to Nanometer Scales.” Nature Biotechnology. Springer
Nature, 2024. https://doi.org/10.1038/s41587-023-01911-8.
ieee: J. M. Michalska et al., “Imaging brain tissue architecture across millimeter
to nanometer scales,” Nature Biotechnology, vol. 42. Springer Nature, pp.
1051–1064, 2024.
ista: Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer
CM, Amberg N, Venturino A, Roessler K, Czech T, Höftberger R, Siegert S, Novarino
G, Jonas PM, Danzl JG. 2024. Imaging brain tissue architecture across millimeter
to nanometer scales. Nature Biotechnology. 42, 1051–1064.
mla: Michalska, Julia M., et al. “Imaging Brain Tissue Architecture across Millimeter
to Nanometer Scales.” Nature Biotechnology, vol. 42, Springer Nature, 2024,
pp. 1051–64, doi:10.1038/s41587-023-01911-8.
short: J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri,
C.M. Sommer, N. Amberg, A. Venturino, K. Roessler, T. Czech, R. Höftberger, S.
Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, Nature Biotechnology 42 (2024) 1051–1064.
corr_author: '1'
date_created: 2023-09-03T22:01:15Z
date_published: 2024-07-01T00:00:00Z
date_updated: 2026-04-14T08:34:35Z
day: '01'
ddc:
- '570'
department:
- _id: SaSi
- _id: GaNo
- _id: PeJo
- _id: JoDa
- _id: Bio
- _id: RySh
doi: 10.1038/s41587-023-01911-8
ec_funded: 1
external_id:
isi:
- '001065254200001'
pmid:
- '37653226'
file:
- access_level: open_access
checksum: 57d5fafb16f02dcb9f7dddb1bd7e2a71
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T07:48:01Z
date_updated: 2025-01-09T07:48:01Z
file_id: '18784'
file_name: 2024_NatureBiotech_Michalska.pdf
file_size: 26065165
relation: main_file
success: 1
file_date_updated: 2025-01-09T07:48:01Z
has_accepted_license: '1'
intvolume: ' 42'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 1051-1064
pmid: 1
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232
name: Molecular Drug Targets
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
- _id: 23889792-32DE-11EA-91FC-C7463DDC885E
grant_number: LS18-022
name: High content imaging to decode human immune cell interactions in health and
allergic disease
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
publication: Nature Biotechnology
publication_identifier:
eissn:
- 1546-1696
issn:
- 1087-0156
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/danzllab/CATS
record:
- id: '18660'
relation: dissertation_contains
status: deleted
- id: '13126'
relation: research_data
status: public
- id: '18674'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Imaging brain tissue architecture across millimeter to nanometer scales
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 42
year: '2024'
...
---
_id: '14795'
abstract:
- lang: eng
text: Metazoan development relies on the formation and remodeling of cell-cell contacts.
Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in
space and time plays a central role in cell-cell contact formation and maturation.
Nevertheless, how this process is mechanistically achieved when new contacts are
formed remains unclear. Here, by building a biomimetic assay composed of progenitor
cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains,
we show that cortical F-actin flows, driven by the depletion of myosin-2 at the
cell contact center, mediate the dynamic reorganization of adhesion receptors
and cell cortex at the contact. E-cadherin-dependent downregulation of the small
GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a
decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2
becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical
tension gradient from the contact rim to its center. This tension gradient, in
turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin
at the contact rim and the progressive redistribution of E-cadherin from the contact
center to the rim. Eventually, this combination of actomyosin downregulation and
flows at the contact determines the characteristic molecular organization, with
E-cadherin and F-actin accumulating at the contact rim, where they are needed
to mechanically link the contractile cortices of the adhering cells.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "We are grateful to Edwin Munro for their feedback and help with
the single particle analysis. We thank members of the Heisenberg and Loose labs
for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH
plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA
for their continuous support, especially Yann Cesbron for assistance with the laser
cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Feyza N
full_name: Arslan, Feyza N
id: 49DA7910-F248-11E8-B48F-1D18A9856A87
last_name: Arslan
orcid: 0000-0001-5809-9566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology.
2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067
apa: Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P.
J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts.
Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067
chicago: Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp
J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell
Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.
ieee: F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg,
“Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current
Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.
ista: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1),
171–182.e8.
mla: Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated
Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8,
doi:10.1016/j.cub.2023.11.067.
short: F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current
Biology 34 (2024) 171–182.e8.
corr_author: '1'
date_created: 2024-01-14T23:00:56Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2025-09-04T11:39:10Z
day: '08'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MaLo
- _id: NanoFab
doi: 10.1016/j.cub.2023.11.067
ec_funded: 1
external_id:
isi:
- '001154500400001'
pmid:
- '38134934'
file:
- access_level: open_access
checksum: 51220b76d72a614208f84bdbfbaf9b72
content_type: application/pdf
creator: dernst
date_created: 2024-01-16T10:53:31Z
date_updated: 2024-01-16T10:53:31Z
file_id: '14813'
file_name: 2024_CurrentBiology_Arslan.pdf
file_size: 5183861
relation: main_file
success: 1
file_date_updated: 2024-01-16T10:53:31Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 171-182.e8
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2024'
...
---
_id: '14846'
abstract:
- lang: eng
text: Contraction and flow of the actin cell cortex have emerged as a common principle
by which cells reorganize their cytoplasm and take shape. However, how these cortical
flows interact with adjacent cytoplasmic components, changing their form and localization,
and how this affects cytoplasmic organization and cell shape remains unclear.
Here we show that in ascidian oocytes, the cooperative activities of cortical
actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive
oocyte cytoplasmic reorganization and shape changes following fertilization. We
show that vegetal-directed cortical actomyosin flows, established upon oocyte
fertilization, lead to both the accumulation of cortical actin at the vegetal
pole of the zygote and compression and local buckling of the adjacent elastic
solid-like myoplasm layer due to friction forces generated at their interface.
Once cortical flows have ceased, the multiple myoplasm buckles resolve into one
larger buckle, which again drives the formation of the contraction pole—a protuberance
of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings
reveal a mechanism where cortical actomyosin network flows determine cytoplasmic
reorganization and cell shape by deforming adjacent cytoplasmic components through
friction forces.
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: NanoFab
acknowledgement: We would like to thank A. McDougall, E. Hannezo and the Heisenberg
lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP
and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific
Service Units of the Institute of Science and Technology Austria through resources
provided by the Electron Microscopy Facility, Imaging and Optics Facility and the
Nanofabrication Facility. This work was supported by a Joint Project Grant from
the FWF (I 3601-B27).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Silvia
full_name: Caballero Mancebo, Silvia
id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
last_name: Caballero Mancebo
orcid: 0000-0002-5223-3346
- first_name: Rushikesh
full_name: Shinde, Rushikesh
last_name: Shinde
- first_name: Madison
full_name: Bolger-Munro, Madison
id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
last_name: Bolger-Munro
orcid: 0000-0002-8176-4824
- first_name: Matilda
full_name: Peruzzo, Matilda
id: 3F920B30-F248-11E8-B48F-1D18A9856A87
last_name: Peruzzo
orcid: 0000-0002-3415-4628
- first_name: Gregory
full_name: Szep, Gregory
id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
last_name: Szep
- first_name: Irene
full_name: Steccari, Irene
id: 2705C766-9FE2-11EA-B224-C6773DDC885E
last_name: Steccari
- first_name: David
full_name: Labrousse Arias, David
id: CD573DF4-9ED3-11E9-9D77-3223E6697425
last_name: Labrousse Arias
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Andrew
full_name: Callan-Jones, Andrew
last_name: Callan-Jones
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine
cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.
Nature Physics. 2024;20:310-321. doi:10.1038/s41567-023-02302-1
apa: Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G.,
Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic
reorganization and shape changes of ascidian oocytes upon fertilization. Nature
Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02302-1
chicago: Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda
Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction
Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes
upon Fertilization.” Nature Physics. Springer Nature, 2024. https://doi.org/10.1038/s41567-023-02302-1.
ieee: S. Caballero Mancebo et al., “Friction forces determine cytoplasmic
reorganization and shape changes of ascidian oocytes upon fertilization,” Nature
Physics, vol. 20. Springer Nature, pp. 310–321, 2024.
ista: Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari
I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg
C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes
of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.
mla: Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization
and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics,
vol. 20, Springer Nature, 2024, pp. 310–21, doi:10.1038/s41567-023-02302-1.
short: S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I.
Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez,
C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.
corr_author: '1'
date_created: 2024-01-21T23:00:57Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T11:48:28Z
day: '01'
ddc:
- '530'
department:
- _id: CaHe
- _id: JoFi
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
doi: 10.1038/s41567-023-02302-1
external_id:
isi:
- '001138880800005'
pmid:
- '38370025'
file:
- access_level: open_access
checksum: 7891ebe7c900ae47469ab127031dd1ec
content_type: application/pdf
creator: dernst
date_created: 2024-07-16T12:12:43Z
date_updated: 2024-07-16T12:12:43Z
file_id: '17267'
file_name: 2024_NaturePhysics_CaballeroMancebo.pdf
file_size: 9897883
relation: main_file
success: 1
file_date_updated: 2024-07-16T12:12:43Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 310-321
pmid: 1
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03601
name: Control of embryonic cleavage pattern
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/
scopus_import: '1'
status: public
title: Friction forces determine cytoplasmic reorganization and shape changes of ascidian
oocytes upon fertilization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...
---
_id: '14926'
author:
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
citation:
ama: Hauschild R. Matlab script for analysis of clone dispersal. 2024. doi:10.15479/AT:ISTA:14926
apa: Hauschild, R. (2024). Matlab script for analysis of clone dispersal. Institute
of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14926
chicago: Hauschild, Robert. “Matlab Script for Analysis of Clone Dispersal.” Institute
of Science and Technology Austria, 2024. https://doi.org/10.15479/AT:ISTA:14926.
ieee: R. Hauschild, “Matlab script for analysis of clone dispersal.” Institute of
Science and Technology Austria, 2024.
ista: Hauschild R. 2024. Matlab script for analysis of clone dispersal, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:14926.
mla: Hauschild, Robert. Matlab Script for Analysis of Clone Dispersal. Institute
of Science and Technology Austria, 2024, doi:10.15479/AT:ISTA:14926.
short: R. Hauschild, (2024).
corr_author: '1'
date_created: 2024-02-02T14:42:26Z
date_published: 2024-02-02T00:00:00Z
date_updated: 2025-09-04T12:10:39Z
day: '02'
ddc:
- '570'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:14926
file:
- access_level: open_access
checksum: df7f358ae19a176cf710c0a802ce31b1
content_type: application/octet-stream
creator: rhauschild
date_created: 2024-02-02T14:40:31Z
date_updated: 2024-02-02T14:40:31Z
file_id: '14927'
file_name: README.md
file_size: 736
relation: main_file
success: 1
- access_level: open_access
checksum: 10194cc11619eccd8f4b24472e465b7f
content_type: application/x-zip-compressed
creator: rhauschild
date_created: 2024-02-02T14:40:31Z
date_updated: 2024-02-02T14:40:31Z
file_id: '14928'
file_name: Supplementary_file_1.zip
file_size: 3543
relation: main_file
success: 1
file_date_updated: 2024-02-02T14:40:31Z
has_accepted_license: '1'
license: https://opensource.org/licenses/MIT
month: '02'
oa: 1
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '15048'
relation: used_in_publication
status: public
status: public
title: Matlab script for analysis of clone dispersal
tmp:
legal_code_url: https://opensource.org/licenses/MIT
name: The MIT License
short: MIT
type: software
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
APC_amount: 11700 EUR
OA_place: publisher
OA_type: hybrid
_id: '14979'
abstract:
- lang: eng
text: Poxviruses are among the largest double-stranded DNA viruses, with members
such as variola virus, monkeypox virus and the vaccination strain vaccinia virus
(VACV). Knowledge about the structural proteins that form the viral core has remained
sparse. While major core proteins have been annotated via indirect experimental
evidence, their structures have remained elusive and they could not be assigned
to individual core features. Hence, which proteins constitute which layers of
the core, such as the palisade layer and the inner core wall, has remained enigmatic.
Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach
in combination with AlphaFold molecular modeling, that trimers formed by the cleavage
product of VACV protein A10 are the key component of the palisade layer. This
allows us to place previously obtained descriptions of protein interactions within
the core wall into perspective and to provide a detailed model of poxvirus core
architecture. Importantly, we show that interactions within A10 trimers are likely
generalizable over members of orthopox- and parapoxviruses.
acknowledged_ssus:
- _id: ScienComp
- _id: LifeSc
- _id: EM-Fac
acknowledgement: "We thank A. Bergthaler (Research Center for Molecular Medicine of
the Austrian Academy of Sciences) for providing VACV WR. We thank A. Nicholas and
his team at the ISTA proteomics facility, and S. Elefante at the ISTA Scientific
Computing facility for their support. We also thank F. Fäßler, D. Porley, T. Muthspiel
and other members of the Schur group for support and helpful discussions. We also
thank D. Castaño-Díez for support with Dynamo. We thank D. Farrell for his help
optimizing the Rosetta protocol to refine the atomic model into the cryo-EM map
with symmetry.\r\n\r\nF.K.M.S. acknowledges support from ISTA and EMBO. F.K.M.S.
also received support from the Austrian Science Fund (FWF) grant P31445. This publication
has been made possible in part by CZI grant DAF2021-234754 and grant https://doi.org/10.37921/812628ebpcwg
from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community
Foundation (funder https://doi.org/10.13039/100014989) awarded to F.K.M.S.\r\n\r\nThis
research was also supported by the Scientific Service Units (SSUs) of ISTA through
resources provided by Scientific Computing (SciComp), the Life Science Facility
(LSF), and the Electron Microscopy Facility (EMF). We also acknowledge the use of
COSMIC45 and Colabfold46."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Julia
full_name: Datler, Julia
id: 3B12E2E6-F248-11E8-B48F-1D18A9856A87
last_name: Datler
orcid: 0000-0002-3616-8580
- first_name: Jesse
full_name: Hansen, Jesse
id: 1063c618-6f9b-11ec-9123-f912fccded63
last_name: Hansen
orcid: 0000-0001-7967-2085
- first_name: Andreas
full_name: Thader, Andreas
id: 3A18A7B8-F248-11E8-B48F-1D18A9856A87
last_name: Thader
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Lukas W
full_name: Bauer, Lukas W
id: 0c894dcf-897b-11ed-a09c-8186353224b0
last_name: Bauer
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
orcid: 0000-0003-3904-947X
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Datler J, Hansen J, Thader A, et al. Multi-modal cryo-EM reveals trimers of
protein A10 to form the palisade layer in poxvirus cores. Nature Structural
& Molecular Biology. 2024;31:1114-1123. doi:10.1038/s41594-023-01201-6
apa: Datler, J., Hansen, J., Thader, A., Schlögl, A., Bauer, L. W., Hodirnau, V.-V.,
& Schur, F. K. (2024). Multi-modal cryo-EM reveals trimers of protein A10
to form the palisade layer in poxvirus cores. Nature Structural & Molecular
Biology. Springer Nature. https://doi.org/10.1038/s41594-023-01201-6
chicago: Datler, Julia, Jesse Hansen, Andreas Thader, Alois Schlögl, Lukas W Bauer,
Victor-Valentin Hodirnau, and Florian KM Schur. “Multi-Modal Cryo-EM Reveals Trimers
of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” Nature Structural
& Molecular Biology. Springer Nature, 2024. https://doi.org/10.1038/s41594-023-01201-6.
ieee: J. Datler et al., “Multi-modal cryo-EM reveals trimers of protein A10
to form the palisade layer in poxvirus cores,” Nature Structural & Molecular
Biology, vol. 31. Springer Nature, pp. 1114–1123, 2024.
ista: Datler J, Hansen J, Thader A, Schlögl A, Bauer LW, Hodirnau V-V, Schur FK.
2024. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade
layer in poxvirus cores. Nature Structural & Molecular Biology. 31, 1114–1123.
mla: Datler, Julia, et al. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to
Form the Palisade Layer in Poxvirus Cores.” Nature Structural & Molecular
Biology, vol. 31, Springer Nature, 2024, pp. 1114–23, doi:10.1038/s41594-023-01201-6.
short: J. Datler, J. Hansen, A. Thader, A. Schlögl, L.W. Bauer, V.-V. Hodirnau,
F.K. Schur, Nature Structural & Molecular Biology 31 (2024) 1114–1123.
corr_author: '1'
date_created: 2024-02-12T09:59:45Z
date_published: 2024-07-01T00:00:00Z
date_updated: 2026-04-07T12:59:44Z
day: '01'
ddc:
- '570'
department:
- _id: FlSc
- _id: ScienComp
- _id: EM-Fac
doi: 10.1038/s41594-023-01201-6
external_id:
isi:
- '001158144600002'
pmid:
- '38316877'
file:
- access_level: open_access
checksum: bda7bf65d81455480efaed8ca293b0db
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T11:27:22Z
date_updated: 2024-07-22T11:27:22Z
file_id: '17307'
file_name: 2024_NatureStrucBio_Datler.pdf
file_size: 17485494
relation: main_file
success: 1
file_date_updated: 2024-07-22T11:27:22Z
has_accepted_license: '1'
intvolume: ' 31'
isi: 1
keyword:
- Molecular Biology
- Structural Biology
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 1114-1123
pmid: 1
project:
- _id: 26736D6A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P31445
name: Structural conservation and diversity in retroviral capsid
publication: Nature Structural & Molecular Biology
publication_identifier:
eissn:
- 1545-9985
issn:
- 1545-9993
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/down-to-the-core-of-poxviruses/
record:
- id: '18766'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer
in poxvirus cores
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 31
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '15018'
abstract:
- lang: eng
text: The epitaxial growth of a strained Ge layer, which is a promising candidate
for the channel material of a hole spin qubit, has been demonstrated on 300 mm
Si wafers using commercially available Si0.3Ge0.7 strain relaxed buffer (SRB)
layers. The assessment of the layer and the interface qualities for a buried strained
Ge layer embedded in Si0.3Ge0.7 layers is reported. The XRD reciprocal space mapping
confirmed that the reduction of the growth temperature enables the 2-dimensional
growth of the Ge layer fully strained with respect to the Si0.3Ge0.7. Nevertheless,
dislocations at the top and/or bottom interface of the Ge layer were observed
by means of electron channeling contrast imaging, suggesting the importance of
the careful dislocation assessment. The interface abruptness does not depend on
the selection of the precursor gases, but it is strongly influenced by the growth
temperature which affects the coverage of the surface H-passivation. The mobility
of 2.7 × 105 cm2/Vs is promising, while the low percolation density of 3 × 1010
/cm2 measured with a Hall-bar device at 7 K illustrates the high quality of the
heterostructure thanks to the high Si0.3Ge0.7 SRB quality.
acknowledgement: The Ge project received funding from the European Union's Horizon
Europe programme under the Grant Agreement 101069515 – IGNITE. Siltronic AG is acknowledged
for providing the SRB wafers. This work was supported by Imec's Industrial Affiliation
Program on Quantum Computing.
article_number: '108231'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Yosuke
full_name: Shimura, Yosuke
last_name: Shimura
- first_name: Clement
full_name: Godfrin, Clement
last_name: Godfrin
- first_name: Andriy
full_name: Hikavyy, Andriy
last_name: Hikavyy
- first_name: Roy
full_name: Li, Roy
last_name: Li
- first_name: Juan L
full_name: Aguilera Servin, Juan L
id: 2A67C376-F248-11E8-B48F-1D18A9856A87
last_name: Aguilera Servin
orcid: 0000-0002-2862-8372
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
- first_name: Paola
full_name: Favia, Paola
last_name: Favia
- first_name: Han
full_name: Han, Han
last_name: Han
- first_name: Danny
full_name: Wan, Danny
last_name: Wan
- first_name: Kristiaan
full_name: de Greve, Kristiaan
last_name: de Greve
- first_name: Roger
full_name: Loo, Roger
last_name: Loo
citation:
ama: Shimura Y, Godfrin C, Hikavyy A, et al. Compressively strained epitaxial Ge
layers for quantum computing applications. Materials Science in Semiconductor
Processing. 2024;174(5). doi:10.1016/j.mssp.2024.108231
apa: Shimura, Y., Godfrin, C., Hikavyy, A., Li, R., Aguilera Servin, J. L., Katsaros,
G., … Loo, R. (2024). Compressively strained epitaxial Ge layers for quantum computing
applications. Materials Science in Semiconductor Processing. Elsevier.
https://doi.org/10.1016/j.mssp.2024.108231
chicago: Shimura, Yosuke, Clement Godfrin, Andriy Hikavyy, Roy Li, Juan L Aguilera
Servin, Georgios Katsaros, Paola Favia, et al. “Compressively Strained Epitaxial
Ge Layers for Quantum Computing Applications.” Materials Science in Semiconductor
Processing. Elsevier, 2024. https://doi.org/10.1016/j.mssp.2024.108231.
ieee: Y. Shimura et al., “Compressively strained epitaxial Ge layers for
quantum computing applications,” Materials Science in Semiconductor Processing,
vol. 174, no. 5. Elsevier, 2024.
ista: Shimura Y, Godfrin C, Hikavyy A, Li R, Aguilera Servin JL, Katsaros G, Favia
P, Han H, Wan D, de Greve K, Loo R. 2024. Compressively strained epitaxial Ge
layers for quantum computing applications. Materials Science in Semiconductor
Processing. 174(5), 108231.
mla: Shimura, Yosuke, et al. “Compressively Strained Epitaxial Ge Layers for Quantum
Computing Applications.” Materials Science in Semiconductor Processing,
vol. 174, no. 5, 108231, Elsevier, 2024, doi:10.1016/j.mssp.2024.108231.
short: Y. Shimura, C. Godfrin, A. Hikavyy, R. Li, J.L. Aguilera Servin, G. Katsaros,
P. Favia, H. Han, D. Wan, K. de Greve, R. Loo, Materials Science in Semiconductor
Processing 174 (2024).
date_created: 2024-02-22T14:10:40Z
date_published: 2024-05-20T00:00:00Z
date_updated: 2025-04-14T08:01:27Z
day: '20'
ddc:
- '530'
department:
- _id: GeKa
- _id: NanoFab
doi: 10.1016/j.mssp.2024.108231
external_id:
isi:
- '001188520000001'
file:
- access_level: open_access
checksum: 62e8e9ae960387a3dca32ec7f5e413ab
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T11:56:08Z
date_updated: 2024-07-22T11:56:08Z
file_id: '17312'
file_name: 2024_MaterialsScience_Shimura.pdf
file_size: 4220165
relation: main_file
success: 1
file_date_updated: 2024-07-22T11:56:08Z
has_accepted_license: '1'
intvolume: ' 174'
isi: 1
issue: '5'
keyword:
- Mechanical Engineering
- Mechanics of Materials
- Condensed Matter Physics
- General Materials Science
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 34c0acea-11ca-11ed-8bc3-8775e10fd452
grant_number: '101069515'
name: Integrated Germanium Quantum Technology
publication: Materials Science in Semiconductor Processing
publication_identifier:
issn:
- 1369-8001
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Compressively strained epitaxial Ge layers for quantum computing applications
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 174
year: '2024'
...
---
_id: '15048'
abstract:
- lang: eng
text: Embryogenesis results from the coordinated activities of different signaling
pathways controlling cell fate specification and morphogenesis. In vertebrate
gastrulation, both Nodal and BMP signaling play key roles in germ layer specification
and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis
is still insufficiently understood. Here, we took a reductionist approach using
zebrafish embryonic explants to study the coordination of Nodal and BMP signaling
for embryo patterning and morphogenesis. We show that Nodal signaling triggers
explant elongation by inducing mesendodermal progenitors but also suppressing
BMP signaling activity at the site of mesendoderm induction. Consistent with this,
ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm
intercalations, key processes during explant elongation. Translating these ex
vivo observations to the intact embryo showed that, similar to explants, Nodal
signaling suppresses the effect of BMP signaling on cell intercalations in the
dorsal domain, thus allowing robust embryonic axis elongation. These findings
suggest a dual function of Nodal signaling in embryonic axis elongation by both
inducing mesendoderm and suppressing BMP effects in the dorsal portion of the
mesendoderm.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Patrick Müller for sharing the chordintt250 mutant zebrafish
line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b
plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated
the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro
and Katherine Rogers and members of the Heisenberg lab for discussions, technical
advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo
for discussions. We thank the Imaging and Optics Facility as well as the Life Science
facility at IST Austria for support with microscopy and fish maintenance.\r\nThis
work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573
to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy
of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience
and Technology Austria. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Kornelija
full_name: Pranjic-Ferscha, Kornelija
id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
last_name: Pranjic-Ferscha
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation
by Nodal-dependent restriction of BMP signaling. Development. 2024;151(4):1-18.
doi:10.1242/dev.202316
apa: Schauer, A., Pranjic-Ferscha, K., Hauschild, R., & Heisenberg, C.-P. J.
(2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling.
Development. The Company of Biologists. https://doi.org/10.1242/dev.202316
chicago: Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp
J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.”
Development. The Company of Biologists, 2024. https://doi.org/10.1242/dev.202316.
ieee: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust
axis elongation by Nodal-dependent restriction of BMP signaling,” Development,
vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.
ista: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis
elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4),
1–18.
mla: Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction
of BMP Signaling.” Development, vol. 151, no. 4, The Company of Biologists,
2024, pp. 1–18, doi:10.1242/dev.202316.
short: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development
151 (2024) 1–18.
corr_author: '1'
date_created: 2024-03-03T23:00:50Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T12:10:40Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1242/dev.202316
ec_funded: 1
external_id:
isi:
- '001170580200001'
pmid:
- '38372390'
file:
- access_level: open_access
checksum: 6961ea10012bf0d266681f9628bb8f13
content_type: application/pdf
creator: dernst
date_created: 2024-03-04T07:24:43Z
date_updated: 2024-03-04T07:24:43Z
file_id: '15050'
file_name: 2024_Development_Schauer.pdf
file_size: 14839986
relation: main_file
success: 1
file_date_updated: 2024-03-04T07:24:43Z
has_accepted_license: '1'
intvolume: ' 151'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1-18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14926'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Robust axis elongation by Nodal-dependent restriction of BMP signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 151
year: '2024'
...
---
_id: '15146'
abstract:
- lang: eng
text: The extracellular matrix (ECM) serves as a scaffold for cells and plays an
essential role in regulating numerous cellular processes, including cell migration
and proliferation. Due to limitations in specimen preparation for conventional
room-temperature electron microscopy, we lack structural knowledge on how ECM
components are secreted, remodeled, and interact with surrounding cells. We have
developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion
beam milling, the lift-out extraction procedure, and cryo-electron tomography.
Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting
in a versatile tool closely mimicking ECM environments. This allows us to visualize
ECM for the first time in its hydrated, native context. Our data reveal an intricate
network of extracellular fibers, their positioning relative to matrix-secreting
cells, and previously unresolved structural entities. Our workflow and results
add to the structural atlas of the ECM, providing novel insights into its secretion
and assembly.
acknowledged_ssus:
- _id: LifeSc
- _id: ScienComp
- _id: EM-Fac
- _id: M-Shop
acknowledgement: "Open Access funding provided by IST Austria. We thank Armel Nicolas
and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante,
and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and
Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the
Miba Machine shop for their support. We also thank Wanda Kukulski (University of
Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group
for helpful discussions. Matt Swulius and Jessica Heebner provided great support
in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure
illustration.\r\n\r\nThis research was supported by the Scientific Service Units
of ISTA through resources provided by Scientific Computing, the Lab Support Facility,
and the Electron Microscopy Facility. We acknowledge funding support from the following
sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation
of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds
(to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the
European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt),
and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been
made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg
from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community
Foundation (to F.K.M. Schur)."
article_number: e202309125
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Bettina
full_name: Zens, Bettina
id: 45FD126C-F248-11E8-B48F-1D18A9856A87
last_name: Zens
orcid: 0000-0002-9561-1239
- first_name: Florian
full_name: Fäßler, Florian
id: 404F5528-F248-11E8-B48F-1D18A9856A87
last_name: Fäßler
orcid: 0000-0001-7149-769X
- first_name: Jesse
full_name: Hansen, Jesse
id: 1063c618-6f9b-11ec-9123-f912fccded63
last_name: Hansen
orcid: 0000-0001-7967-2085
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Julia
full_name: Datler, Julia
id: 3B12E2E6-F248-11E8-B48F-1D18A9856A87
last_name: Datler
orcid: 0000-0002-3616-8580
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
orcid: 0000-0003-3904-947X
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Jonna H
full_name: Alanko, Jonna H
id: 2CC12E8C-F248-11E8-B48F-1D18A9856A87
last_name: Alanko
orcid: 0000-0002-7698-3061
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal
the ultrastructural landscape of extracellular matrix. Journal of Cell Biology.
2024;223(6). doi:10.1083/jcb.202309125
apa: Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V.,
… Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural
landscape of extracellular matrix. Journal of Cell Biology. Rockefeller
University Press. https://doi.org/10.1083/jcb.202309125
chicago: Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler,
Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and
Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural
Landscape of Extracellular Matrix.” Journal of Cell Biology. Rockefeller
University Press, 2024. https://doi.org/10.1083/jcb.202309125.
ieee: B. Zens et al., “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural
landscape of extracellular matrix,” Journal of Cell Biology, vol. 223,
no. 6. Rockefeller University Press, 2024.
ista: Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V,
Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the
ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6),
e202309125.
mla: Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural
Landscape of Extracellular Matrix.” Journal of Cell Biology, vol. 223,
no. 6, e202309125, Rockefeller University Press, 2024, doi:10.1083/jcb.202309125.
short: B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V.
Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).
corr_author: '1'
date_created: 2024-03-21T06:45:51Z
date_published: 2024-03-20T00:00:00Z
date_updated: 2025-09-04T13:17:16Z
day: '20'
ddc:
- '570'
department:
- _id: FlSc
- _id: MiSi
- _id: Bio
- _id: EM-Fac
doi: 10.1083/jcb.202309125
ec_funded: 1
external_id:
isi:
- '001264190100001'
pmid:
- '38506714'
file:
- access_level: open_access
checksum: 90d1984a93660735e506c2a304bc3f73
content_type: application/pdf
creator: dernst
date_created: 2024-03-25T12:52:04Z
date_updated: 2024-03-25T12:52:04Z
file_id: '15188'
file_name: 2024_JCB_Zens.pdf
file_size: 11907016
relation: main_file
success: 1
file_date_updated: 2024-03-25T12:52:04Z
has_accepted_license: '1'
intvolume: ' 223'
isi: 1
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 9B954C5C-BA93-11EA-9121-9846C619BF3A
grant_number: P33367
name: Structure and isoform diversity of the Arp2/3 complex
- _id: 7bd318a1-9f16-11ee-852c-cc9217763180
grant_number: E435
name: In Situ Actin Structures via Hybrid Cryo-electron Microscopy
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular Navigation Along Spatial Gradients
- _id: 059B463C-7A3F-11EA-A408-12923DDC885E
name: "NÃ\x96-Fonds Preis für die Jungforscherin des Jahres am IST Austria"
- _id: 2615199A-B435-11E9-9278-68D0E5697425
grant_number: '21317'
name: Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis
- _id: 62909c6f-2b32-11ec-9570-e1476aab5308
grant_number: CZI01
name: CryoMinflux-guided in-situ visual proteomics and structure determination
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular
matrix
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 223
year: '2024'
...
---
_id: '15164'
abstract:
- lang: eng
text: Primary implant stability, which refers to the stability of the implant during
the initial healing period is a crucial factor in determining the long-term success
of the implant and lays the foundation for secondary implant stability achieved
through osseointegration. Factors affecting primary stability include implant
design, surgical technique, and patient-specific factors like bone quality and
morphology. In vivo, the cyclic nature of anatomical loading puts osteosynthesis
locking screws under dynamic loads, which can lead to the formation of micro cracks
and defects that slowly degrade the mechanical connection between the bone and
screw, thus compromising the initial stability and secondary stability of the
implant. Monotonic quasi-static loading used for testing the holding capacity
of implanted screws is not well suited to capture this behavior since it cannot
capture the progressive deterioration of peri‑implant bone at small displacements.
In order to address this issue, this study aims to determine a critical point
of loss of primary implant stability in osteosynthesis locking screws under cyclic
overloading by investigating the evolution of damage, dissipated energy, and permanent
deformation. A custom-made test setup was used to test implanted 2.5 mm locking
screws under cyclic overloading test. For each loading cycle, maximum forces and
displacement were recorded as well as initial and final cycle displacements and
used to calculate damage and energy dissipation evolution. The results of this
study demonstrate that for axial, shear, and mixed loading significant damage
and energy dissipation can be observed at approximately 20 % of the failure force.
Additionally, at this load level, permanent deformations on the screw-bone interface
were found to be in the range of 50 to 150 mm which promotes osseointegration
and secondary implant stability. This research can assist surgeons in making informed
preoperative decisions by providing a better understanding of the critical point
of loss of primary implant stability, thus improving the long-term success of
the implant and overall patient satisfaction.
acknowledgement: The authors declare no conflict of interest related to this study.
This project was funded by the Gesellschaft fuer Forschungsfoerderung Niederoesterreich
m.b.H. Life Science Call 2017 Grant No. LS17004 and Science call 2019 Dissertationen
Grant No. SC19014. No ethical approval was required for this study.
article_number: '104143'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Juan D.
full_name: Silva-Henao, Juan D.
last_name: Silva-Henao
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
- first_name: Dieter H.
full_name: Pahr, Dieter H.
last_name: Pahr
- first_name: Andreas G.
full_name: Reisinger, Andreas G.
last_name: Reisinger
citation:
ama: Silva-Henao JD, Schober S, Pahr DH, Reisinger AG. Critical loss of primary
implant stability in osteosynthesis locking screws under cyclic overloading. Medical
Engineering and Physics. 2024;126. doi:10.1016/j.medengphy.2024.104143
apa: Silva-Henao, J. D., Schober, S., Pahr, D. H., & Reisinger, A. G. (2024).
Critical loss of primary implant stability in osteosynthesis locking screws under
cyclic overloading. Medical Engineering and Physics. Elsevier. https://doi.org/10.1016/j.medengphy.2024.104143
chicago: Silva-Henao, Juan D., Sophie Schober, Dieter H. Pahr, and Andreas G. Reisinger.
“Critical Loss of Primary Implant Stability in Osteosynthesis Locking Screws under
Cyclic Overloading.” Medical Engineering and Physics. Elsevier, 2024. https://doi.org/10.1016/j.medengphy.2024.104143.
ieee: J. D. Silva-Henao, S. Schober, D. H. Pahr, and A. G. Reisinger, “Critical
loss of primary implant stability in osteosynthesis locking screws under cyclic
overloading,” Medical Engineering and Physics, vol. 126. Elsevier, 2024.
ista: Silva-Henao JD, Schober S, Pahr DH, Reisinger AG. 2024. Critical loss of primary
implant stability in osteosynthesis locking screws under cyclic overloading. Medical
Engineering and Physics. 126, 104143.
mla: Silva-Henao, Juan D., et al. “Critical Loss of Primary Implant Stability in
Osteosynthesis Locking Screws under Cyclic Overloading.” Medical Engineering
and Physics, vol. 126, 104143, Elsevier, 2024, doi:10.1016/j.medengphy.2024.104143.
short: J.D. Silva-Henao, S. Schober, D.H. Pahr, A.G. Reisinger, Medical Engineering
and Physics 126 (2024).
date_created: 2024-03-24T23:00:58Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2025-09-04T13:11:03Z
day: '01'
ddc:
- '610'
department:
- _id: PreCl
doi: 10.1016/j.medengphy.2024.104143
external_id:
isi:
- '001219145400001'
pmid:
- '38621845'
file:
- access_level: open_access
checksum: 974acbf2731e7382dcf5920ac762e551
content_type: application/pdf
creator: dernst
date_created: 2024-03-25T08:29:52Z
date_updated: 2024-03-25T08:29:52Z
file_id: '15177'
file_name: 2024_MedEngineeringPhysics_SilvaHenao.pdf
file_size: 10039402
relation: main_file
success: 1
file_date_updated: 2024-03-25T08:29:52Z
has_accepted_license: '1'
intvolume: ' 126'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Medical Engineering and Physics
publication_identifier:
eissn:
- 1873-4030
issn:
- 1350-4533
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Critical loss of primary implant stability in osteosynthesis locking screws
under cyclic overloading
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 126
year: '2024'
...
---
_id: '15182'
abstract:
- lang: eng
text: Thermoelectric materials convert heat into electricity, with a broad range
of applications near room temperature (RT). However, the library of RT high-performance
materials is limited. Traditional high-temperature synthetic methods constrain
the range of materials achievable, hindering the ability to surpass crystal structure
limitations and engineer defects. Here, a solution-based synthetic approach is
introduced, enabling RT synthesis of powders and exploration of densification
at lower temperatures to influence the material's microstructure. The approach
is exemplified by Ag2Se, an n-type alternative to bismuth telluride. It is demonstrated
that the concentration of Ag interstitials, grain boundaries, and dislocations
are directly correlated to the sintering temperature, and achieve a figure of
merit of 1.1 from RT to 100 °C after optimization. Moreover, insights into and
resolve Ag2Se's challenges are provided, including stoichiometry issues leading
to irreproducible performances. This work highlights the potential of RT solution
synthesis in expanding the repertoire of high-performance thermoelectric materials
for practical applications.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: NanoFab
acknowledgement: This work was supported by the Scientific Service Units (SSU) of
ISTA through resources provided by the Electron Microscopy Facility (EMF), the Lab
Support Facility (LSF), and the Nanofabrication Facility (NNF). This work was financially
supported by ISTA and the Werner Siemens Foundation. The USTEM Service Unit of the
Technical University of Vienna is acknowledged for EBSD sample preparation and analysis.
R.L.B. acknowledges the National Science Foundation for funding the mass spectrometry
analysis under award DMR 1904719. J.L. is a Serra Húnter Fellow and is grateful
to the ICREA Academia program and projects MICINN/FEDER PID2021-124572OB-C31 and
GC 2021 SGR 01061.
article_number: '2400408'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Tobias
full_name: Kleinhanns, Tobias
id: 8BD9DE16-AB3C-11E9-9C8C-2A03E6697425
last_name: Kleinhanns
orcid: 0000-0003-1537-7436
- first_name: Francesco
full_name: Milillo, Francesco
id: 38b830db-ea88-11ee-bf9b-929beaf79054
last_name: Milillo
- first_name: Mariano
full_name: Calcabrini, Mariano
id: 45D7531A-F248-11E8-B48F-1D18A9856A87
last_name: Calcabrini
orcid: 0000-0003-4566-5877
- first_name: Christine
full_name: Fiedler, Christine
id: bd3fceba-dc74-11ea-a0a7-c17f71817366
last_name: Fiedler
- first_name: Sharona
full_name: Horta, Sharona
id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc
last_name: Horta
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Marissa J.
full_name: Strumolo, Marissa J.
last_name: Strumolo
- first_name: Roger
full_name: Hasler, Roger
last_name: Hasler
- first_name: Jordi
full_name: Llorca, Jordi
last_name: Llorca
- first_name: Michael
full_name: Tkadletz, Michael
last_name: Tkadletz
- first_name: Richard L.
full_name: Brutchey, Richard L.
last_name: Brutchey
- first_name: Maria
full_name: Ibáñez, Maria
id: 43C61214-F248-11E8-B48F-1D18A9856A87
last_name: Ibáñez
orcid: 0000-0001-5013-2843
citation:
ama: 'Kleinhanns T, Milillo F, Calcabrini M, et al. A route to high thermoelectric
performance: Solution‐based control of microstructure and composition in Ag2Se.
Advanced Energy Materials. 2024;14(22). doi:10.1002/aenm.202400408'
apa: 'Kleinhanns, T., Milillo, F., Calcabrini, M., Fiedler, C., Horta, S., Balazs,
D., … Ibáñez, M. (2024). A route to high thermoelectric performance: Solution‐based
control of microstructure and composition in Ag2Se. Advanced Energy Materials.
Wiley. https://doi.org/10.1002/aenm.202400408'
chicago: 'Kleinhanns, Tobias, Francesco Milillo, Mariano Calcabrini, Christine Fiedler,
Sharona Horta, Daniel Balazs, Marissa J. Strumolo, et al. “A Route to High Thermoelectric
Performance: Solution‐based Control of Microstructure and Composition in Ag2Se.”
Advanced Energy Materials. Wiley, 2024. https://doi.org/10.1002/aenm.202400408.'
ieee: 'T. Kleinhanns et al., “A route to high thermoelectric performance:
Solution‐based control of microstructure and composition in Ag2Se,” Advanced
Energy Materials, vol. 14, no. 22. Wiley, 2024.'
ista: 'Kleinhanns T, Milillo F, Calcabrini M, Fiedler C, Horta S, Balazs D, Strumolo
MJ, Hasler R, Llorca J, Tkadletz M, Brutchey RL, Ibáñez M. 2024. A route to high
thermoelectric performance: Solution‐based control of microstructure and composition
in Ag2Se. Advanced Energy Materials. 14(22), 2400408.'
mla: 'Kleinhanns, Tobias, et al. “A Route to High Thermoelectric Performance: Solution‐based
Control of Microstructure and Composition in Ag2Se.” Advanced Energy Materials,
vol. 14, no. 22, 2400408, Wiley, 2024, doi:10.1002/aenm.202400408.'
short: T. Kleinhanns, F. Milillo, M. Calcabrini, C. Fiedler, S. Horta, D. Balazs,
M.J. Strumolo, R. Hasler, J. Llorca, M. Tkadletz, R.L. Brutchey, M. Ibáñez, Advanced
Energy Materials 14 (2024).
corr_author: '1'
date_created: 2024-03-25T08:57:40Z
date_published: 2024-06-12T00:00:00Z
date_updated: 2026-06-22T06:14:35Z
day: '12'
ddc:
- '530'
department:
- _id: MaIb
- _id: LifeSc
doi: 10.1002/aenm.202400408
external_id:
isi:
- '001184300200001'
file:
- access_level: open_access
checksum: 86b26430e00d5f43ea19e9b610692ab7
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T12:07:56Z
date_updated: 2024-07-22T12:07:56Z
file_id: '17314'
file_name: 2024_AdvancedEnergyMaterials_Kleinhanns.pdf
file_size: 8824301
relation: main_file
success: 1
file_date_updated: 2024-07-22T12:07:56Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '22'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A
name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of
Semiconductors for Waste Heat Recovery'
publication: Advanced Energy Materials
publication_identifier:
eissn:
- 1614-6840
issn:
- 1614-6832
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '22017'
relation: dissertation_contains
status: for_moderation
scopus_import: '1'
status: public
title: 'A route to high thermoelectric performance: Solution‐based control of microstructure
and composition in Ag2Se'
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 14
year: '2024'
...
---
_id: '15385'
abstract:
- lang: eng
text: "Relevant information about the data can be found in the 'Readme_Data.txt'
file. \r\nA previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4\r\nand
published in Plos Biology (2024)"
acknowledged_ssus:
- _id: PreCl
- _id: M-Shop
- _id: LifeSc
- _id: Bio
acknowledgement: 'We thank Armel Nicolas, Bella Bruszel and Ewelina Dutkiewicz from
the ISTA Mass Spectrometry Service (Lab Services Facilities) for all Proteomics
work, including samples preparation, LC/MS data acquisition, searches and data evaluation.
We thank Prof. Peter Jonas for his suggestion on the involvement of potassium channels
and members of the Neuroethology group for their comments on the manuscript. Katalin
Szigeti and Julie Murmann for experimental help. This research was supported by
the Scientific Service Units of ISTA through resources provided by the Lab Support
Facility, the Imaging and Optics Facility, the Machine Shop Unit and the Preclinical
Facility, especially Freyja Langer and Michael Schunn. '
article_processing_charge: No
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Tomas
full_name: Masson, Tomas
id: 93ac43e8-8599-11eb-9b86-f6efb0a4c207
last_name: Masson
orcid: 0000-0002-2634-6283
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Burnett L, Koppensteiner P, Symonova O, et al. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. 2024.
doi:10.15479/AT:ISTA:15385
apa: Burnett, L., Koppensteiner, P., Symonova, O., Masson, T., Vega Zuniga, T. A.,
Contreras, X., … Jösch, M. A. (2024). Shared behavioural impairments in visual
perception and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:15385
chicago: Burnett, Laura, Peter Koppensteiner, Olga Symonova, Tomas Masson, Tomas
A Vega Zuniga, Ximena Contreras, Thomas Rülicke, Ryuichi Shigemoto, Gaia Novarino,
and Maximilian A Jösch. “Shared Behavioural Impairments in Visual Perception and
Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey
Hypoexcitability in Setd5 Haploinsufficient Mice.” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/AT:ISTA:15385.
ieee: L. Burnett et al., “Shared behavioural impairments in visual perception
and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice.” Institute of Science and
Technology Austria, 2024.
ista: Burnett L, Koppensteiner P, Symonova O, Masson T, Vega Zuniga TA, Contreras
X, Rülicke T, Shigemoto R, Novarino G, Jösch MA. 2024. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:15385.
mla: Burnett, Laura, et al. Shared Behavioural Impairments in Visual Perception
and Place Avoidance across Different Autism Models Are Driven by Periaqueductal
Grey Hypoexcitability in Setd5 Haploinsufficient Mice. Institute of Science
and Technology Austria, 2024, doi:10.15479/AT:ISTA:15385.
short: L. Burnett, P. Koppensteiner, O. Symonova, T. Masson, T.A. Vega Zuniga, X.
Contreras, T. Rülicke, R. Shigemoto, G. Novarino, M.A. Jösch, (2024).
corr_author: '1'
date_created: 2024-05-13T15:04:04Z
date_published: 2024-05-15T00:00:00Z
date_updated: 2025-09-08T07:57:11Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PreCl
- _id: SiHi
- _id: RySh
- _id: GaNo
doi: 10.15479/AT:ISTA:15385
file:
- access_level: open_access
checksum: 9205eb0876f0f08552dbad80d6884b4b
content_type: application/zip
creator: mjoesch
date_created: 2024-05-15T06:09:17Z
date_updated: 2024-05-15T06:09:17Z
file_id: '15396'
file_name: PatchClamp.zip
file_size: '1149617663'
relation: main_file
success: 1
- access_level: open_access
content_type: application/zip
creator: mjoesch
date_created: 2024-05-15T06:09:12Z
date_updated: 2024-05-15T06:09:12Z
file_id: '15397'
file_name: SiliconProbe.zip
file_size: '564903112'
relation: main_file
success: 1
- access_level: open_access
checksum: 49a807bbab06b5fada38f532e2176e2e
content_type: application/zip
creator: mjoesch
date_created: 2024-05-15T06:09:14Z
date_updated: 2024-05-15T06:09:14Z
file_id: '15398'
file_name: WesternBlot.zip
file_size: '11685703'
relation: main_file
success: 1
- access_level: open_access
checksum: beeeeaa43770090f3b291209ed6b0623
content_type: application/zip
creator: mjoesch
date_created: 2024-05-15T06:09:38Z
date_updated: 2024-05-15T06:09:38Z
file_id: '15399'
file_name: Behaviour.zip
file_size: '1335626779'
relation: main_file
success: 1
- access_level: open_access
checksum: 8862ad7719388304d1d19f8e7db8bb00
content_type: text/plain
creator: mjoesch
date_created: 2024-05-16T09:08:20Z
date_updated: 2024-05-16T09:08:20Z
file_id: '15400'
file_name: Readme_Data.txt
file_size: 18841
relation: main_file
success: 1
file_date_updated: 2024-05-16T09:08:20Z
has_accepted_license: '1'
keyword:
- ASD
- periaqueductal gray
- perception
- behavior
- potassium channels
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '05'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17142'
relation: used_in_publication
status: public
status: public
title: Shared behavioural impairments in visual perception and place avoidance across
different autism models are driven by periaqueductal grey hypoexcitability in Setd5
haploinsufficient mice
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2024'
...
---
_id: '17139'
article_processing_charge: No
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Waleed
full_name: Khalid, Waleed
id: 097c0562-3cf0-11ee-8fd3-e7a79c1e2fd1
last_name: Khalid
- first_name: Stefano
full_name: Elefante, Stefano
id: 490F40CE-F248-11E8-B48F-1D18A9856A87
last_name: Elefante
- first_name: Stephan
full_name: Stadlbauer, Stephan
id: 4D0BC184-F248-11E8-B48F-1D18A9856A87
last_name: Stadlbauer
citation:
ama: 'Schlögl A, Khalid W, Elefante S, Stadlbauer S. How much memory per CPU core
is requested? In: ASHPC24 - Austrian-Slovenian HPC Meeting 2024. EuroCC
Austria; 2024:46. doi:10.25365/phaidra.463'
apa: 'Schlögl, A., Khalid, W., Elefante, S., & Stadlbauer, S. (2024). How much
memory per CPU core is requested? In ASHPC24 - Austrian-Slovenian HPC Meeting
2024 (p. 46). Grundlsee, Austria: EuroCC Austria. https://doi.org/10.25365/phaidra.463'
chicago: Schlögl, Alois, Waleed Khalid, Stefano Elefante, and Stephan Stadlbauer.
“How Much Memory per CPU Core Is Requested?” In ASHPC24 - Austrian-Slovenian
HPC Meeting 2024, 46. EuroCC Austria, 2024. https://doi.org/10.25365/phaidra.463.
ieee: A. Schlögl, W. Khalid, S. Elefante, and S. Stadlbauer, “How much memory per
CPU core is requested?,” in ASHPC24 - Austrian-Slovenian HPC Meeting 2024,
Grundlsee, Austria, 2024, p. 46.
ista: 'Schlögl A, Khalid W, Elefante S, Stadlbauer S. 2024. How much memory per
CPU core is requested? ASHPC24 - Austrian-Slovenian HPC Meeting 2024. ASHPC: Austrian-Slovenian
HPC Meeting, 46.'
mla: Schlögl, Alois, et al. “How Much Memory per CPU Core Is Requested?” ASHPC24
- Austrian-Slovenian HPC Meeting 2024, EuroCC Austria, 2024, p. 46, doi:10.25365/phaidra.463.
short: A. Schlögl, W. Khalid, S. Elefante, S. Stadlbauer, in:, ASHPC24 - Austrian-Slovenian
HPC Meeting 2024, EuroCC Austria, 2024, p. 46.
conference:
end_date: 2024-06-13
location: Grundlsee, Austria
name: 'ASHPC: Austrian-Slovenian HPC Meeting'
start_date: 2024-06-10
date_created: 2024-06-14T09:06:36Z
date_published: 2024-06-13T00:00:00Z
date_updated: 2024-06-17T09:40:37Z
day: '13'
ddc:
- '000'
department:
- _id: ScienComp
doi: 10.25365/phaidra.463
file:
- access_level: open_access
checksum: f7d3dded6df2dcdb4818904cf2e1c183
content_type: application/pdf
creator: dernst
date_created: 2024-06-17T09:36:51Z
date_updated: 2024-06-17T09:36:51Z
file_id: '17153'
file_name: 2024_ASHPC_Schloegl.pdf
file_size: 206746
relation: main_file
success: 1
file_date_updated: 2024-06-17T09:36:51Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '46'
publication: ASHPC24 - Austrian-Slovenian HPC Meeting 2024
publication_identifier:
isbn:
- '9783200096455'
publication_status: published
publisher: EuroCC Austria
quality_controlled: '1'
status: public
title: How much memory per CPU core is requested?
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18581'
abstract:
- lang: eng
text: "Background: Human induced pluripotent stem cells represent a scalable source
of youthful tissue progenitors and secretomes for regenerative therapies. The
aim of our study was to investigate the potential of conditioned medium (CM) from
hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation
of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated
whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could
enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13
donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures
and the effects on proliferation and osteogenic differentiation were examined
after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared
with the activity of MSC-derived CM and with the activity of CM prepared from
hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative
proteomic analysis of CM was performed to gain insight into the molecular components
responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived
MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage
differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline
phosphatase activity of MSC from several adult/aged donors after 14 days of continuous
supplementation under osteogenic conditions. However, CM supplementation failed
to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions.
hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than
CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not
osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity.
Quantitative proteomic analysis of d14-CM revealed a distinct profile of components
that formed a highly interconnected associations network with two clusters, one
functionally associated with binding and organization of actin/cytoskeletal components
and the other with structural constituents of the extracellular matrix, collagen,
and growth factor binding. Several hub proteins were identified that were reported
to have functions in cell-extracellular matrix interaction, osteogenic differentiation
and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early
osteogenic differentiation of human bone marrow-derived MSCs and that prolonged
cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis
of the upregulated protein components provides the basis for further optimization
of hiPSC-MP-CM for bone regenerative therapies."
acknowledgement: "We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for
providing the human tissue waste for primary cell isolation and the New York Stem
Cell Foundation Research Institute for providing the human induced pluripotent stem
cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues
at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing
support of the project. InstaText writing tool (https://instatext.io) was used to
edit the English language of the final manuscript.\r\nThis work has received funding
from the European Union’s Horizon 2020 research and innovation program under the
Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming
European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well
as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and
853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951)
and the Production of the Future program (grant agreement No. 877452)."
article_number: '434'
article_processing_charge: Yes
article_type: original
author:
- first_name: Darja
full_name: Marolt Presen, Darja
last_name: Marolt Presen
- first_name: Vanessa
full_name: Goeschl, Vanessa
last_name: Goeschl
- first_name: Dominik
full_name: Hanetseder, Dominik
last_name: Hanetseder
- first_name: Laura
full_name: Ogrin, Laura
last_name: Ogrin
- first_name: Alexandra Larissa
full_name: Stetco, Alexandra Larissa
last_name: Stetco
- first_name: Anja
full_name: Tansek, Anja
last_name: Tansek
- first_name: Laura
full_name: Pozenel, Laura
last_name: Pozenel
- first_name: Bella
full_name: Bruszel, Bella
id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
last_name: Bruszel
- first_name: Goran
full_name: Mitulovic, Goran
last_name: Mitulovic
- first_name: Johannes
full_name: Oesterreicher, Johannes
last_name: Oesterreicher
- first_name: Johannes
full_name: Zipperle, Johannes
last_name: Zipperle
- first_name: Barbara
full_name: Schaedl, Barbara
last_name: Schaedl
- first_name: Wolfgang
full_name: Holnthoner, Wolfgang
last_name: Holnthoner
- first_name: Johannes
full_name: Grillari, Johannes
last_name: Grillari
- first_name: Heinz
full_name: Redl, Heinz
last_name: Redl
citation:
ama: Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances
the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium.
Stem Cell Research and Therapy. 2024;15. doi:10.1186/s13287-024-03960-5
apa: Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek,
A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity
of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research
and Therapy. Springer Nature. https://doi.org/10.1186/s13287-024-03960-5
chicago: Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin,
Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation
Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned
Medium.” Stem Cell Research and Therapy. Springer Nature, 2024. https://doi.org/10.1186/s13287-024-03960-5.
ieee: D. Marolt Presen et al., “Prolonged cultivation enhances the stimulatory
activity of hiPSC mesenchymal progenitor-derived conditioned medium,” Stem
Cell Research and Therapy, vol. 15. Springer Nature, 2024.
ista: Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel
L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner
W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity
of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research
and Therapy. 15, 434.
mla: Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory
Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” Stem
Cell Research and Therapy, vol. 15, 434, Springer Nature, 2024, doi:10.1186/s13287-024-03960-5.
short: D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek,
L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl,
W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).
date_created: 2024-11-24T23:01:47Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2025-09-09T11:41:12Z
day: '01'
ddc:
- '570'
department:
- _id: LifeSc
doi: 10.1186/s13287-024-03960-5
external_id:
isi:
- '001356479400001'
pmid:
- '39551765'
file:
- access_level: open_access
checksum: 91edba8edde30d781dce89fdd5cadc39
content_type: application/pdf
creator: dernst
date_created: 2024-12-10T08:28:17Z
date_updated: 2024-12-10T08:28:17Z
file_id: '18641'
file_name: 2024_StemCellResearch_Presen.pdf
file_size: 6690494
relation: main_file
success: 1
file_date_updated: 2024-12-10T08:28:17Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Stem Cell Research and Therapy
publication_identifier:
eissn:
- 1757-6512
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal
progenitor-derived conditioned medium
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18587'
abstract:
- lang: eng
text: Many scientific breakthroughs have depended on animal research, yet the ethical
concerns surrounding the use of animals in experimentation have long prompted
discussions about humane treatment and responsible scientific practice. First
articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction,
and Refinement have gained widespread recognition as basic guidelines for animal
research. Over time, the 3Rs have transcended the research community, influencing
policy decisions, animal welfare advocacy and public perception of animal experimentation.
Despite their broad acceptance, interpretations of the 3Rs vary substantially,
shaping statutory frameworks at various levels, with both technical and practical
impacts.
acknowledgement: This publication is based upon work from the Ethics Crossover Group
within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical
science”), CA21139, supported by COST (European Cooperation in Science and Technology).
We acknowledge the input and advice from Dr. Susanna Louhimies.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Jan
full_name: Lauwereyns, Jan
last_name: Lauwereyns
- first_name: Jeffrey
full_name: Bajramovic, Jeffrey
last_name: Bajramovic
- first_name: Bettina
full_name: Bert, Bettina
last_name: Bert
- first_name: Samuel
full_name: Camenzind, Samuel
last_name: Camenzind
- first_name: Joery
full_name: De Kock, Joery
last_name: De Kock
- first_name: Alisa
full_name: Elezović, Alisa
last_name: Elezović
- first_name: Sevilay
full_name: Erden, Sevilay
last_name: Erden
- first_name: Fernando
full_name: Gonzalez-Uarquin, Fernando
last_name: Gonzalez-Uarquin
- first_name: Yesim Isil
full_name: Ulman, Yesim Isil
last_name: Ulman
- first_name: Orsolya Ivett
full_name: Hoffmann, Orsolya Ivett
last_name: Hoffmann
- first_name: Maria
full_name: Kitsara, Maria
last_name: Kitsara
- first_name: Nikolaos
full_name: Kostomitsopoulos, Nikolaos
last_name: Kostomitsopoulos
- first_name: Winfried
full_name: Neuhaus, Winfried
last_name: Neuhaus
- first_name: Benoit
full_name: Petit-Demouliere, Benoit
last_name: Petit-Demouliere
- first_name: Simone
full_name: Pollo, Simone
last_name: Pollo
- first_name: Brígida
full_name: Riso, Brígida
last_name: Riso
- first_name: Sophie
full_name: Schober, Sophie
id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8
last_name: Schober
- first_name: Athanassia
full_name: Sotiropoulos, Athanassia
last_name: Sotiropoulos
- first_name: Aurélie
full_name: Thomas, Aurélie
last_name: Thomas
- first_name: Augusto
full_name: Vitale, Augusto
last_name: Vitale
- first_name: Doris
full_name: Wilflingseder, Doris
last_name: Wilflingseder
- first_name: Arti
full_name: Ahluwalia, Arti
last_name: Ahluwalia
citation:
ama: Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of
the 3Rs principles in animal research. Lab Animal. 2024;53:347-350. doi:10.1038/s41684-024-01476-2
apa: Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović,
A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles
in animal research. Lab Animal. Springer Nature. https://doi.org/10.1038/s41684-024-01476-2
chicago: Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery
De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation
of the 3Rs Principles in Animal Research.” Lab Animal. Springer Nature,
2024. https://doi.org/10.1038/s41684-024-01476-2.
ieee: J. Lauwereyns et al., “Toward a common interpretation of the 3Rs principles
in animal research,” Lab Animal, vol. 53. Springer Nature, pp. 347–350,
2024.
ista: Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden
S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus
W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale
A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs
principles in animal research. Lab Animal. 53, 347–350.
mla: Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles
in Animal Research.” Lab Animal, vol. 53, Springer Nature, 2024, pp. 347–50,
doi:10.1038/s41684-024-01476-2.
short: J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović,
S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos,
W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos,
A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.
date_created: 2024-11-24T23:01:49Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2025-09-08T14:50:31Z
day: '01'
ddc:
- '570'
department:
- _id: PreCl
doi: 10.1038/s41684-024-01476-2
external_id:
isi:
- '001355264100001'
pmid:
- '39548348'
file:
- access_level: open_access
checksum: 67fc140f761581a291591f075e49b88d
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T14:07:04Z
date_updated: 2024-12-03T14:07:04Z
file_id: '18614'
file_name: 2024_LabAnimal_Lauwereyns.pdf
file_size: 967252
relation: main_file
success: 1
file_date_updated: 2024-12-03T14:07:04Z
has_accepted_license: '1'
intvolume: ' 53'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 347-350
pmid: 1
publication: Lab Animal
publication_identifier:
eissn:
- 1548-4475
issn:
- 0093-7355
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Toward a common interpretation of the 3Rs principles in animal research
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 53
year: '2024'
...
---
APC_amount: 6248,82 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18603'
abstract:
- lang: eng
text: It is widely believed that information storage in neuronal circuits involves
nanoscopic structural changes at synapses, resulting in the formation of synaptic
engrams. However, direct evidence for this hypothesis is lacking. To test this
conjecture, we combined chemical potentiation, functional analysis by paired pre-postsynaptic
recordings, and structural analysis by electron microscopy (EM) and freeze-fracture
replica labeling (FRL) at the rodent hippocampal mossy fiber synapse, a key synapse
in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic
transmission revealed that forskolin-induced chemical potentiation increased the
readily releasable vesicle pool size and vesicular release probability by 146%
and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL
demonstrated an increase in the number of vesicles close to the plasma membrane
and the number of clusters of the priming protein Munc13-1, indicating an increase
in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed
a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels,
suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our
results indicate that presynaptic plasticity is associated with structural reorganization
of active zones. We propose that changes in potential nanoscopic organization
at synaptic vesicle release sites may be correlates of learning and memory at
a plastic central synapse.
acknowledged_ssus:
- _id: EM-Fac
- _id: PreCl
acknowledgement: "We thank Carolina Borges-Merjane, Jing-Jing Chen, Katharina Lichter,
and Samuel Young for critically reading the manuscript; the Electron Microscopy
Facility of ISTA, in particular Vanessa Zheden, for extensive support, advice, and
experimental assistance; the Preclinical Facility of ISTA, in particular Victoria
Wimmer and Michael Schunn, for experimental assistance; Florian Marr and Christina
Altmutter for technical support; Alois Schlögl for help with analysis; and Eleftheria
Kralli-Beller for manuscript editing. We also thank Cordelia Imig for providing
Munc13-1cKO-Munc13-2/3(−/−) mutant mice. Part of the work has been published in
O.K.’s thesis in partial fulfillment of the requirements for the degree of Doctor
of Philosophy.\r\nThis project received funding from the European Research Council
and European Union’s Horizon 2020 research and innovation programme (ERC 692692
to P.J.; https://cordis.europa.eu/project/id/692692/de) and from the Fond zur Förderung
der Wissenschaftlichen Forschung (Z312-B27 Wittgenstein award to P.J., https://www.fwf.ac.at/en/funding/portfolio/projects/fwf-wittgenstein-award;
W1205-B09 and P36232-B to P.J., https://www.fwf.ac.at/en/funding; I6166-B to R.S.;
https://www.fwf.ac.at/en/funding). The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the manuscript."
article_number: e3002879
article_processing_charge: Yes
article_type: original
author:
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
orcid: 0000-0003-2344-1039
- first_name: Yuji
full_name: Okamoto, Yuji
id: 3337E116-F248-11E8-B48F-1D18A9856A87
last_name: Okamoto
orcid: 0000-0003-0408-6094
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Nils
full_name: Brose, Nils
last_name: Brose
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. Presynaptic cAMP-PKA-mediated
potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle
coupling at hippocampal mossy fiber boutons. PLoS Biology. 2024;22(11).
doi:10.1371/journal.pbio.3002879
apa: Kim, O., Okamoto, Y., Kaufmann, W., Brose, N., Shigemoto, R., & Jonas,
P. M. (2024). Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration
of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber
boutons. PLoS Biology. Public Library of Science. https://doi.org/10.1371/journal.pbio.3002879
chicago: Kim, Olena, Yuji Okamoto, Walter Kaufmann, Nils Brose, Ryuichi Shigemoto,
and Peter M Jonas. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration
of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber
Boutons.” PLoS Biology. Public Library of Science, 2024. https://doi.org/10.1371/journal.pbio.3002879.
ieee: O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, and P. M. Jonas,
“Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic
vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons,”
PLoS Biology, vol. 22, no. 11. Public Library of Science, 2024.
ista: Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. 2024. Presynaptic
cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools
and channel-vesicle coupling at hippocampal mossy fiber boutons. PLoS Biology.
22(11), e3002879.
mla: Kim, Olena, et al. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration
of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber
Boutons.” PLoS Biology, vol. 22, no. 11, e3002879, Public Library of Science,
2024, doi:10.1371/journal.pbio.3002879.
short: O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, P.M. Jonas, PLoS
Biology 22 (2024).
corr_author: '1'
date_created: 2024-12-01T23:01:54Z
date_published: 2024-11-18T00:00:00Z
date_updated: 2026-04-16T12:20:34Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
- _id: EM-Fac
- _id: RySh
doi: 10.1371/journal.pbio.3002879
ec_funded: 1
external_id:
isi:
- '001358568700003'
pmid:
- '39556620'
file:
- access_level: open_access
checksum: 7de2dcb50deb65dde05c80082bb85a82
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T08:56:53Z
date_updated: 2024-12-03T08:56:53Z
file_id: '18608'
file_name: 2024_PloSBio_Kim.pdf
file_size: 3057631
relation: main_file
success: 1
file_date_updated: 2024-12-03T08:56:53Z
has_accepted_license: '1'
intvolume: ' 22'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
- _id: b1b85715-d554-11ed-a5ad-84a07fc9f18e
grant_number: I06166
name: Structural & functional basis of presynaptic plasticity
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
call_identifier: FWF
name: FWF Open Access Fund
publication: PLoS Biology
publication_identifier:
eissn:
- 1545-7885
issn:
- 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
record:
- id: '18296'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic
vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 22
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18920'
abstract:
- lang: eng
text: The globally distributed marine alga Emiliania huxleyi has cooling effect
on the Earth’s climate. The population density of E. huxleyi is restricted by
Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite
the impact of E. huxleyi viruses on the climate, there is limited information
about their structure and replication. Here, we show that the dsDNA genome inside
the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane.
EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse
the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble
in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments.
Genome packaging initiates synchronously with the capsid assembly and completes
through an aperture in the forming capsid. The genome-filled capsids acquire an
outer membrane by budding into intracellular vesicles. EhV-201 infection induces
a loss of surface protective layers from E. huxleyi cells, which enables the continuous
release of virions by exocytosis.
acknowledgement: We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core
Facility and Proteomics Core Facility of the Central European Institute of Technology
(CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and
Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility
supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR);
and (iii) Plant Sciences Core Facility for support with obtaining scientific data
presented here. We acknowledge support from the project National Institute of Virology
and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European
Union - Next Generation EU. This work received funding from the Czech Science Foundation
grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI”
(no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship
funded by Brno city municipality to M.H.
article_number: 'eadk1954 '
article_processing_charge: Yes
article_type: original
author:
- first_name: Miroslav
full_name: Homola, Miroslav
last_name: Homola
- first_name: Renate Carina
full_name: Büttner, Renate Carina
id: 3b7984c9-17ff-11ed-b6fe-f943c4a5b626
last_name: Büttner
- first_name: Tibor
full_name: Füzik, Tibor
last_name: Füzik
- first_name: Pavel
full_name: Křepelka, Pavel
last_name: Křepelka
- first_name: Radka
full_name: Holbová, Radka
last_name: Holbová
- first_name: Jiří
full_name: Nováček, Jiří
last_name: Nováček
- first_name: Marten L.
full_name: Chaillet, Marten L.
last_name: Chaillet
- first_name: Jakub
full_name: Žák, Jakub
last_name: Žák
- first_name: Danyil
full_name: Grybchuk, Danyil
last_name: Grybchuk
- first_name: Friedrich
full_name: Förster, Friedrich
last_name: Förster
- first_name: William H.
full_name: Wilson, William H.
last_name: Wilson
- first_name: Declan C.
full_name: Schroeder, Declan C.
last_name: Schroeder
- first_name: Pavel
full_name: Plevka, Pavel
last_name: Plevka
citation:
ama: Homola M, Büttner RC, Füzik T, et al. Structure and replication cycle of a
virus infecting climate-modulating alga Emiliania huxleyi. Science Advances.
2024;10(15). doi:10.1126/sciadv.adk1954
apa: Homola, M., Büttner, R. C., Füzik, T., Křepelka, P., Holbová, R., Nováček,
J., … Plevka, P. (2024). Structure and replication cycle of a virus infecting
climate-modulating alga Emiliania huxleyi. Science Advances. American Association
for the Advancement of Science. https://doi.org/10.1126/sciadv.adk1954
chicago: Homola, Miroslav, Renate Carina Büttner, Tibor Füzik, Pavel Křepelka, Radka
Holbová, Jiří Nováček, Marten L. Chaillet, et al. “Structure and Replication Cycle
of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” Science Advances.
American Association for the Advancement of Science, 2024. https://doi.org/10.1126/sciadv.adk1954.
ieee: M. Homola et al., “Structure and replication cycle of a virus infecting
climate-modulating alga Emiliania huxleyi,” Science Advances, vol. 10,
no. 15. American Association for the Advancement of Science, 2024.
ista: Homola M, Büttner RC, Füzik T, Křepelka P, Holbová R, Nováček J, Chaillet
ML, Žák J, Grybchuk D, Förster F, Wilson WH, Schroeder DC, Plevka P. 2024. Structure
and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi.
Science Advances. 10(15), eadk1954.
mla: Homola, Miroslav, et al. “Structure and Replication Cycle of a Virus Infecting
Climate-Modulating Alga Emiliania Huxleyi.” Science Advances, vol. 10,
no. 15, eadk1954, American Association for the Advancement of Science, 2024, doi:10.1126/sciadv.adk1954.
short: M. Homola, R.C. Büttner, T. Füzik, P. Křepelka, R. Holbová, J. Nováček, M.L.
Chaillet, J. Žák, D. Grybchuk, F. Förster, W.H. Wilson, D.C. Schroeder, P. Plevka,
Science Advances 10 (2024).
date_created: 2025-01-27T14:32:34Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2025-05-14T09:29:04Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.1126/sciadv.adk1954
external_id:
pmid:
- '38598627'
file:
- access_level: open_access
checksum: 291dd7ceccbe6bfd8e0a9157584f88e9
content_type: application/pdf
creator: dernst
date_created: 2025-01-27T14:40:08Z
date_updated: 2025-01-27T14:40:08Z
file_id: '18921'
file_name: 2024_ScienceAdv_Homola.pdf
file_size: 40623405
relation: main_file
success: 1
file_date_updated: 2025-01-27T14:40:08Z
has_accepted_license: '1'
intvolume: ' 10'
issue: '15'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
link:
- relation: software
url: ' https://github.com/fuzikt/tomostarpy.'
scopus_import: '1'
status: public
title: Structure and replication cycle of a virus infecting climate-modulating alga
Emiliania huxleyi
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '19520'
abstract:
- lang: eng
text: Vertebrates exhibit a wide range of motor behaviors, ranging from swimming
to complex limb-based movements. Here we take advantage of frog metamorphosis,
which captures a swim-to-limb-based movement transformation during the development
of a single organism, to explore changes in the underlying spinal circuits. We
find that the tadpole spinal cord contains small and largely homogeneous populations
of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages.
These neuronal populations only modestly increase in number and subtype heterogeneity
with the emergence of free swimming. In contrast, during frog metamorphosis and
the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron
number and transcriptional heterogeneity, culminating in cohorts of neurons that
exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated
gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively,
results in severe but selective deficits in tail and limb function. Our work thus
demonstrates that neural diversity scales exponentially with increasing behavioral
complexity and illustrates striking evolutionary conservation in the molecular
organization and function of motor circuits across species.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We would like to thank the members of the Sweeney Lab (especially
Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project
and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion,
and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their
scientific insight and mentorship during the conception of this project. This\r\nproject
would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena
Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA).
In addition, we thank our funding sources for providing the resources to do these\r\nexperiments:
FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon
Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch
Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS
5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.);
NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities
(ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472
(Z.H.); and Project A.L.S. (S.B-M.). "
article_processing_charge: No
author:
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: 'Florina Alexandra '
full_name: 'Toma, Florina Alexandra '
id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
last_name: Toma
- first_name: Zoe P
full_name: Harrington, Zoe P
id: a8144562-32c9-11ee-b5ce-d9800628bda2
last_name: Harrington
orcid: 0009-0008-0158-4032
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Alexandra J.
full_name: Trevisan, Alexandra J.
last_name: Trevisan
- first_name: Phillip
full_name: Chapman, Phillip
last_name: Chapman
- first_name: Mara
full_name: Julseth, Mara
id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
last_name: Julseth
- first_name: Susan
full_name: Brenner-Morton, Susan
last_name: Brenner-Morton
- first_name: Mariano I.
full_name: Gabitto, Mariano I.
last_name: Gabitto
- first_name: Jeremy S.
full_name: Dasen, Jeremy S.
last_name: Dasen
- first_name: Jay B.
full_name: Bikoff, Jay B.
last_name: Bikoff
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales
exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv.
doi:10.1101/2024.09.20.614050
apa: Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R.,
Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis. bioRxiv. https://doi.org/10.1101/2024.09.20.614050
chicago: Vijatovic, David, Florina Alexandra Toma, Zoe P Harrington, Christoph
M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal
Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during
Frog Metamorphosis.” BioRxiv, n.d. https://doi.org/10.1101/2024.09.20.614050.
ieee: D. Vijatovic et al., “Spinal neuron diversity scales exponentially
with swim-to-limb transformation during frog metamorphosis,” bioRxiv. .
ista: Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ,
Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney
LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis. bioRxiv, 10.1101/2024.09.20.614050.
mla: Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with
Swim-to-Limb Transformation during Frog Metamorphosis.” BioRxiv, doi:10.1101/2024.09.20.614050.
short: D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J.
Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen,
J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2025-04-07T08:48:28Z
date_published: 2024-09-27T00:00:00Z
date_updated: 2025-05-14T11:40:13Z
day: '27'
department:
- _id: LoSw
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1101/2024.09.20.614050
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.09.20.614050
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Development of V1 interneuron diversity during swim-to-walk transition of
Xenopus metamorphosis
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
grant_number: CZI01
name: Tools for automation and feedback microscopy
publication: bioRxiv
publication_status: submitted
status: public
title: Spinal neuron diversity scales exponentially with swim-to-limb transformation
during frog metamorphosis
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
APC_amount: 804 EUR
OA_place: publisher
OA_type: gold
_id: '17232'
abstract:
- lang: eng
text: "The lineage relationship of clonally-related cells offers important insights
into the ontogeny and cytoarchitecture of the brain in health and disease. Here,
we provide a protocol to concurrently assess cell lineage relationship and cell-type
identity among clonally-related cells in situ. We first describe the preparation
and screening of acute brain slices containing clonally-related cells labeled
using mosaic analysis with double markers (MADM). We then outline steps to collect
RNA from individual cells for downstream applications and cell-type identification
using RNA sequencing.\r\nFor complete details on the use and execution of this
protocol, please refer to Cheung et al.\r\n1"
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
- _id: PreCl
acknowledgement: We thank R. Beattie and T. Asenov for designing and producing components
of the multi-well slice recover chamber. We thank R. Shigemoto for providing equipment
access. We thank C. Streicher and A. Heger for mouse breeding support. This work
was supported by the Scientific Service Units of IST Austria through resources provided
by the Imaging & Optics, Miba Machine Shop, and Preclinical facilities. G.C. received
funding from the European Commission (IST plus postdoctoral fellowship) and S.H.
was funded by ISTA institutional funds and the Austrian Science Fund Special Research
Programmes (FWF SFB-F78 Neuro Stem Modulation).
article_number: '103168'
article_processing_charge: Yes
article_type: original
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Cheung GT, Pauler F, Koppensteiner P, Hippenmeyer S. Protocol for mapping cell
lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.
STAR Protocols. 2024;5(3). doi:10.1016/j.xpro.2024.103168
apa: Cheung, G. T., Pauler, F., Koppensteiner, P., & Hippenmeyer, S. (2024).
Protocol for mapping cell lineage and cell-type identity of clonally-related cells
in situ using MADM-CloneSeq. STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2024.103168
chicago: Cheung, Giselle T, Florian Pauler, Peter Koppensteiner, and Simon Hippenmeyer.
“Protocol for Mapping Cell Lineage and Cell-Type Identity of Clonally-Related
Cells in Situ Using MADM-CloneSeq.” STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2024.103168.
ieee: G. T. Cheung, F. Pauler, P. Koppensteiner, and S. Hippenmeyer, “Protocol for
mapping cell lineage and cell-type identity of clonally-related cells in situ
using MADM-CloneSeq,” STAR Protocols, vol. 5, no. 3. Elsevier, 2024.
ista: Cheung GT, Pauler F, Koppensteiner P, Hippenmeyer S. 2024. Protocol for mapping
cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.
STAR Protocols. 5(3), 103168.
mla: Cheung, Giselle T., et al. “Protocol for Mapping Cell Lineage and Cell-Type
Identity of Clonally-Related Cells in Situ Using MADM-CloneSeq.” STAR Protocols,
vol. 5, no. 3, 103168, Elsevier, 2024, doi:10.1016/j.xpro.2024.103168.
short: G.T. Cheung, F. Pauler, P. Koppensteiner, S. Hippenmeyer, STAR Protocols
5 (2024).
corr_author: '1'
date_created: 2024-07-14T22:01:10Z
date_published: 2024-09-20T00:00:00Z
date_updated: 2025-12-30T10:54:12Z
day: '20'
ddc:
- '570'
department:
- _id: SiHi
- _id: PreCl
doi: 10.1016/j.xpro.2024.103168
external_id:
pmid:
- '38968076'
file:
- access_level: open_access
checksum: 464f52ecc6ec92f509552823bb82bf79
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T12:16:53Z
date_updated: 2025-01-09T12:16:53Z
file_id: '18810'
file_name: 2024_STARProtoc_Cheung2.pdf
file_size: 6445556
relation: main_file
success: 1
file_date_updated: 2025-01-09T12:16:53Z
has_accepted_license: '1'
intvolume: ' 5'
issue: '3'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: STAR Protocols
publication_identifier:
eissn:
- 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for mapping cell lineage and cell-type identity of clonally-related
cells in situ using MADM-CloneSeq
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17280'
abstract:
- lang: eng
text: Adherens junction–associated protein 1 (AJAP1) has been implicated in brain
diseases; however, a pathogenic mechanism has not been identified. AJAP1 is widely
expressed in neurons and binds to γ-aminobutyric acid type B receptors (GBRs),
which inhibit neurotransmitter release at most synapses in the brain. Here, we
show that AJAP1 is selectively expressed in dendrites and trans-synaptically recruits
GBRs to presynaptic sites of neurons expressing AJAP1. We have identified several
monoallelic AJAP1 variants in individuals with epilepsy and/or neurodevelopmental
disorders. Specifically, we show that the variant p.(W183C) lacks binding to GBRs,
resulting in the inability to recruit them. Ultrastructural analysis revealed
significantly decreased presynaptic GBR levels in Ajap1−/− and Ajap1W183C/+ mice.
Consequently, these mice exhibited reduced GBR-mediated presynaptic inhibition
at excitatory and inhibitory synapses, along with impaired synaptic plasticity.
Our study reveals that AJAP1 enables the postsynaptic neuron to regulate the level
of presynaptic GBR-mediated inhibition, supporting the clinical relevance of loss-of-function
AJAP1 variants.
acknowledgement: "Ajap1HA/HA and Ajap1W183C/+ mice were generated in collaboration
with Pawel Pelczar at the center for transgenic models at the University of Basel,
Switzerland. We thank the imaging core facility (IMCF, University of Basel) and
in particular A. Ferrand for the technical assistance provided on the OMX 3D-SIM
microscope.\r\nThis work was supported by a grant from the Swiss National Science
Foundation (SNF) to B.B. (31003A-152970, 310030B-201291), an NIH grant to E.A. and
E.H.S. (R01NS058721), DFG grants to B.F. (TRR 152 project ID 239283807, FA 332/15-1,
16-1), and grants to P.S. from AIMS-2-TRIALS, which are supported by the Innovative
Medicines Initiatives from the European Commission joint undertaking under grant
agreement No 777394."
article_number: adk5462
article_processing_charge: Yes
article_type: original
author:
- first_name: Simon
full_name: Früh, Simon
last_name: Früh
- first_name: Sami
full_name: Boudkkazi, Sami
last_name: Boudkkazi
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Vita
full_name: Sereikaite, Vita
last_name: Sereikaite
- first_name: Li Yuan
full_name: Chen, Li Yuan
last_name: Chen
- first_name: Diego
full_name: Fernandez-Fernandez, Diego
last_name: Fernandez-Fernandez
- first_name: Pascal D.
full_name: Rem, Pascal D.
last_name: Rem
- first_name: Daniel
full_name: Ulrich, Daniel
last_name: Ulrich
- first_name: Jochen
full_name: Schwenk, Jochen
last_name: Schwenk
- first_name: Ziyang
full_name: Chen, Ziyang
last_name: Chen
- first_name: Elodie Le
full_name: Monnier, Elodie Le
last_name: Monnier
- first_name: Thorsten
full_name: Fritzius, Thorsten
last_name: Fritzius
- first_name: Sabrina M.
full_name: Innocenti, Sabrina M.
last_name: Innocenti
- first_name: Valérie
full_name: Besseyrias, Valérie
last_name: Besseyrias
- first_name: Luca
full_name: Trovò, Luca
last_name: Trovò
- first_name: Michal
full_name: Stawarski, Michal
last_name: Stawarski
- first_name: Emanuela
full_name: Argilli, Emanuela
last_name: Argilli
- first_name: Elliott H.
full_name: Sherr, Elliott H.
last_name: Sherr
- first_name: Bregje
full_name: Van Bon, Bregje
last_name: Van Bon
- first_name: Erik Jan
full_name: Kamsteeg, Erik Jan
last_name: Kamsteeg
- first_name: Maria
full_name: Iascone, Maria
last_name: Iascone
- first_name: Alba
full_name: Pilotta, Alba
last_name: Pilotta
- first_name: Maria R.
full_name: Cutrì, Maria R.
last_name: Cutrì
- first_name: Mahshid S.
full_name: Azamian, Mahshid S.
last_name: Azamian
- first_name: Andrés
full_name: Hernández-García, Andrés
last_name: Hernández-García
- first_name: Seema R.
full_name: Lalani, Seema R.
last_name: Lalani
- first_name: Jill A.
full_name: Rosenfeld, Jill A.
last_name: Rosenfeld
- first_name: Xiaonan
full_name: Zhao, Xiaonan
last_name: Zhao
- first_name: Tiphanie P.
full_name: Vogel, Tiphanie P.
last_name: Vogel
- first_name: Herda
full_name: Ona, Herda
last_name: Ona
- first_name: Daryl A.
full_name: Scott, Daryl A.
last_name: Scott
- first_name: Peter
full_name: Scheiffele, Peter
last_name: Scheiffele
- first_name: Kristian
full_name: Strømgaard, Kristian
last_name: Strømgaard
- first_name: Mehdi
full_name: Tafti, Mehdi
last_name: Tafti
- first_name: Martin
full_name: Gassmann, Martin
last_name: Gassmann
- first_name: Bernd
full_name: Fakler, Bernd
last_name: Fakler
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Bernhard
full_name: Bettler, Bernhard
last_name: Bettler
citation:
ama: Früh S, Boudkkazi S, Koppensteiner P, et al. Monoallelic de novo AJAP1 loss-of-
function variants disrupt trans-synaptic control of neurotransmitter release.
Science Advances. 2024;10(28). doi:10.1126/sciadv.adk5462
apa: Früh, S., Boudkkazi, S., Koppensteiner, P., Sereikaite, V., Chen, L. Y., Fernandez-Fernandez,
D., … Bettler, B. (2024). Monoallelic de novo AJAP1 loss-of- function variants
disrupt trans-synaptic control of neurotransmitter release. Science Advances.
American Association for the Advancement of Science. https://doi.org/10.1126/sciadv.adk5462
chicago: Früh, Simon, Sami Boudkkazi, Peter Koppensteiner, Vita Sereikaite, Li Yuan
Chen, Diego Fernandez-Fernandez, Pascal D. Rem, et al. “Monoallelic de Novo AJAP1
Loss-of- Function Variants Disrupt Trans-Synaptic Control of Neurotransmitter
Release.” Science Advances. American Association for the Advancement of
Science, 2024. https://doi.org/10.1126/sciadv.adk5462.
ieee: S. Früh et al., “Monoallelic de novo AJAP1 loss-of- function variants
disrupt trans-synaptic control of neurotransmitter release,” Science Advances,
vol. 10, no. 28. American Association for the Advancement of Science, 2024.
ista: Früh S, Boudkkazi S, Koppensteiner P, Sereikaite V, Chen LY, Fernandez-Fernandez
D, Rem PD, Ulrich D, Schwenk J, Chen Z, Monnier EL, Fritzius T, Innocenti SM,
Besseyrias V, Trovò L, Stawarski M, Argilli E, Sherr EH, Van Bon B, Kamsteeg EJ,
Iascone M, Pilotta A, Cutrì MR, Azamian MS, Hernández-García A, Lalani SR, Rosenfeld
JA, Zhao X, Vogel TP, Ona H, Scott DA, Scheiffele P, Strømgaard K, Tafti M, Gassmann
M, Fakler B, Shigemoto R, Bettler B. 2024. Monoallelic de novo AJAP1 loss-of-
function variants disrupt trans-synaptic control of neurotransmitter release.
Science Advances. 10(28), adk5462.
mla: Früh, Simon, et al. “Monoallelic de Novo AJAP1 Loss-of- Function Variants Disrupt
Trans-Synaptic Control of Neurotransmitter Release.” Science Advances,
vol. 10, no. 28, adk5462, American Association for the Advancement of Science,
2024, doi:10.1126/sciadv.adk5462.
short: S. Früh, S. Boudkkazi, P. Koppensteiner, V. Sereikaite, L.Y. Chen, D. Fernandez-Fernandez,
P.D. Rem, D. Ulrich, J. Schwenk, Z. Chen, E.L. Monnier, T. Fritzius, S.M. Innocenti,
V. Besseyrias, L. Trovò, M. Stawarski, E. Argilli, E.H. Sherr, B. Van Bon, E.J.
Kamsteeg, M. Iascone, A. Pilotta, M.R. Cutrì, M.S. Azamian, A. Hernández-García,
S.R. Lalani, J.A. Rosenfeld, X. Zhao, T.P. Vogel, H. Ona, D.A. Scott, P. Scheiffele,
K. Strømgaard, M. Tafti, M. Gassmann, B. Fakler, R. Shigemoto, B. Bettler, Science
Advances 10 (2024).
date_created: 2024-07-21T22:01:01Z
date_published: 2024-07-12T00:00:00Z
date_updated: 2025-09-08T08:15:54Z
day: '12'
ddc:
- '570'
department:
- _id: RySh
- _id: PreCl
doi: 10.1126/sciadv.adk5462
external_id:
isi:
- '001280159000022'
pmid:
- '38985877'
file:
- access_level: open_access
checksum: 9cbc4501fcd4ba1c0811fd244031422b
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T06:29:27Z
date_updated: 2024-07-22T06:29:27Z
file_id: '17287'
file_name: 2024_ScienceAdv_Früh.pdf
file_size: 7241489
relation: main_file
success: 1
file_date_updated: 2024-07-22T06:29:27Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
issue: '28'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Monoallelic de novo AJAP1 loss-of- function variants disrupt trans-synaptic
control of neurotransmitter release
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 10
year: '2024'
...
---
_id: '17284'
abstract:
- lang: eng
text: Platelet homeostasis is essential for vascular integrity and immune defence1,2.
Although the process of platelet formation by fragmenting megakaryocytes (MKs;
thrombopoiesis) has been extensively studied, the cellular and molecular mechanisms
required to constantly replenish the pool of MKs by their progenitor cells (megakaryopoiesis)
remains unclear3,4. Here we use intravital imaging to track the cellular dynamics
of megakaryopoiesis over days. We identify plasmacytoid dendritic cells (pDCs)
as homeostatic sensors that monitor the bone marrow for apoptotic MKs and deliver
IFNα to the MK niche triggering local on-demand proliferation and maturation of
MK progenitors. This pDC-dependent feedback loop is crucial for MK and platelet
homeostasis at steady state and under stress. pDCs are best known for their ability
to function as vigilant detectors of viral infection5. We show that virus-induced
activation of pDCs interferes with their function as homeostatic sensors of megakaryopoiesis.
Consequently, activation of pDCs by SARS-CoV-2 leads to excessive megakaryopoiesis.
Together, we identify a pDC-dependent homeostatic circuit that involves innate
immune sensing and demand-adapted release of inflammatory mediators to maintain
homeostasis of the megakaryocytic lineage.
acknowledgement: 'We thank S. Helmer, N. Blount, E. Raatz and Z. Sisic for technical
assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German
Research Foundation) SFB 1123 (S.M. project B06); SFB 914 (S.M. projects B02 and
Z01, H.I.-A. project Z01, S.S. project A06, K.S. project B02, C. Schulz project
A10, B.W. project A02, C. Scheiermann project B09); SFB 1054 (T.B. project B03);
FOR2033 (F.G., R.A.J.O., S.M.); Individual research grant project ID: 514478744
(F.G.); Heisenberg Programme project ID: 514477451 (F.G.); the DZHK (German Center
for Cardiovascular Research) (MHA 1.4VD (S.M.), Postdoc Start-up Grant, 81×3600213
(F.G.)); and LMUexcellence NFF (F.G.). W.F. received funding from China Scholarship
Council (CSC, no. 201306270012). P.B. is supported by the German Research Foundation
(DFG, project IDs 322900939, 432698239 and 445703531), European Research Council
(ERC Consolidator grant no. 101001791) and the Federal Ministry of Education and
Research (BMBF, STOP-FSGS-01GM2202C and NATON within the framework of the Network
of University Medicine, no. 01KX2121). S.v.S. is supported by the START-Program
of the Faculty of Medicine of the RWTH Aachen University (AZ 125/17). A.D. and S.E.
are supported by the German Research Foundation (SFB TRR 267); S.E. by the BMBF
in the framework of the Cluster4future program (CNATM—Cluster for Nucleic Acid Therapeutics
Munich). This project has received funding from the European Research Council (ERC)
under the European Union’s Horizon 2020 research and innovation programme (grant
agreement no. 833440 to S.M.). F.G. received funding from the European Union’s Horizon
2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement
no. 747687. The project is funded by the European Union (ERC, MEKanics, 101078110).
Views and opinions expressed are those of the author(s) only and do not necessarily
reflect those of the European Union or the European Research Council Executive Agency.
Neither the European Union nor the granting authority can be held responsible for
them.'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
- first_name: Hellen
full_name: Ishikawa-Ankerhold, Hellen
last_name: Ishikawa-Ankerhold
- first_name: Susanne
full_name: Stutte, Susanne
last_name: Stutte
- first_name: Wenwen
full_name: Fu, Wenwen
last_name: Fu
- first_name: Jutta
full_name: Weitz, Jutta
last_name: Weitz
- first_name: Anne
full_name: Dueck, Anne
last_name: Dueck
- first_name: Bhavishya
full_name: Nelakuditi, Bhavishya
last_name: Nelakuditi
- first_name: Valeria
full_name: Fumagalli, Valeria
last_name: Fumagalli
- first_name: Dominic
full_name: Van Den Heuvel, Dominic
last_name: Van Den Heuvel
- first_name: Larissa
full_name: Belz, Larissa
last_name: Belz
- first_name: Gulnoza
full_name: Sobirova, Gulnoza
last_name: Sobirova
- first_name: Zhe
full_name: Zhang, Zhe
last_name: Zhang
- first_name: Anna
full_name: Titova, Anna
last_name: Titova
- first_name: Alejandro Martinez
full_name: Navarro, Alejandro Martinez
last_name: Navarro
- first_name: Kami
full_name: Pekayvaz, Kami
last_name: Pekayvaz
- first_name: Michael
full_name: Lorenz, Michael
last_name: Lorenz
- first_name: Louisa
full_name: Von Baumgarten, Louisa
last_name: Von Baumgarten
- first_name: Jan
full_name: Kranich, Jan
last_name: Kranich
- first_name: Tobias
full_name: Straub, Tobias
last_name: Straub
- first_name: Bastian
full_name: Popper, Bastian
last_name: Popper
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Chenglong
full_name: Guo, Chenglong
last_name: Guo
- first_name: Guido
full_name: Piontek, Guido
last_name: Piontek
- first_name: Saskia
full_name: Von Stillfried, Saskia
last_name: Von Stillfried
- first_name: Peter
full_name: Boor, Peter
last_name: Boor
- first_name: Marco
full_name: Colonna, Marco
last_name: Colonna
- first_name: Sebastian
full_name: Clauß, Sebastian
last_name: Clauß
- first_name: Christian
full_name: Schulz, Christian
last_name: Schulz
- first_name: Thomas
full_name: Brocker, Thomas
last_name: Brocker
- first_name: Barbara
full_name: Walzog, Barbara
last_name: Walzog
- first_name: Christoph
full_name: Scheiermann, Christoph
last_name: Scheiermann
- first_name: William C.
full_name: Aird, William C.
last_name: Aird
- first_name: Claus
full_name: Nerlov, Claus
last_name: Nerlov
- first_name: Konstantin
full_name: Stark, Konstantin
last_name: Stark
- first_name: Tobias
full_name: Petzold, Tobias
last_name: Petzold
- first_name: Stefan
full_name: Engelhardt, Stefan
last_name: Engelhardt
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Martina
full_name: Rudelius, Martina
last_name: Rudelius
- first_name: Robert A.J.
full_name: Oostendorp, Robert A.J.
last_name: Oostendorp
- first_name: Matteo
full_name: Iannacone, Matteo
last_name: Iannacone
- first_name: Matthias
full_name: Heinig, Matthias
last_name: Heinig
- first_name: Steffen
full_name: Massberg, Steffen
last_name: Massberg
citation:
ama: Gärtner FR, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells
control homeostasis of megakaryopoiesis. Nature. 2024;631:645-653. doi:10.1038/s41586-024-07671-y
apa: Gärtner, F. R., Ishikawa-Ankerhold, H., Stutte, S., Fu, W., Weitz, J., Dueck,
A., … Massberg, S. (2024). Plasmacytoid dendritic cells control homeostasis of
megakaryopoiesis. Nature. Springer Nature. https://doi.org/10.1038/s41586-024-07671-y
chicago: Gärtner, Florian R, Hellen Ishikawa-Ankerhold, Susanne Stutte, Wenwen Fu,
Jutta Weitz, Anne Dueck, Bhavishya Nelakuditi, et al. “Plasmacytoid Dendritic
Cells Control Homeostasis of Megakaryopoiesis.” Nature. Springer Nature,
2024. https://doi.org/10.1038/s41586-024-07671-y.
ieee: F. R. Gärtner et al., “Plasmacytoid dendritic cells control homeostasis
of megakaryopoiesis,” Nature, vol. 631. Springer Nature, pp. 645–653, 2024.
ista: Gärtner FR, Ishikawa-Ankerhold H, Stutte S, Fu W, Weitz J, Dueck A, Nelakuditi
B, Fumagalli V, Van Den Heuvel D, Belz L, Sobirova G, Zhang Z, Titova A, Navarro
AM, Pekayvaz K, Lorenz M, Von Baumgarten L, Kranich J, Straub T, Popper B, Zheden
V, Kaufmann W, Guo C, Piontek G, Von Stillfried S, Boor P, Colonna M, Clauß S,
Schulz C, Brocker T, Walzog B, Scheiermann C, Aird WC, Nerlov C, Stark K, Petzold
T, Engelhardt S, Sixt MK, Hauschild R, Rudelius M, Oostendorp RAJ, Iannacone M,
Heinig M, Massberg S. 2024. Plasmacytoid dendritic cells control homeostasis of
megakaryopoiesis. Nature. 631, 645–653.
mla: Gärtner, Florian R., et al. “Plasmacytoid Dendritic Cells Control Homeostasis
of Megakaryopoiesis.” Nature, vol. 631, Springer Nature, 2024, pp. 645–53,
doi:10.1038/s41586-024-07671-y.
short: F.R. Gärtner, H. Ishikawa-Ankerhold, S. Stutte, W. Fu, J. Weitz, A. Dueck,
B. Nelakuditi, V. Fumagalli, D. Van Den Heuvel, L. Belz, G. Sobirova, Z. Zhang,
A. Titova, A.M. Navarro, K. Pekayvaz, M. Lorenz, L. Von Baumgarten, J. Kranich,
T. Straub, B. Popper, V. Zheden, W. Kaufmann, C. Guo, G. Piontek, S. Von Stillfried,
P. Boor, M. Colonna, S. Clauß, C. Schulz, T. Brocker, B. Walzog, C. Scheiermann,
W.C. Aird, C. Nerlov, K. Stark, T. Petzold, S. Engelhardt, M.K. Sixt, R. Hauschild,
M. Rudelius, R.A.J. Oostendorp, M. Iannacone, M. Heinig, S. Massberg, Nature 631
(2024) 645–653.
corr_author: '1'
date_created: 2024-07-21T22:01:02Z
date_published: 2024-07-18T00:00:00Z
date_updated: 2025-09-08T08:14:25Z
day: '18'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: MiSi
- _id: Bio
doi: 10.1038/s41586-024-07671-y
ec_funded: 1
external_id:
isi:
- '001281636500020'
pmid:
- '38987596'
file:
- access_level: open_access
checksum: aa004afc72d2489f0fb0fcbc9919fbbd
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T06:16:11Z
date_updated: 2024-07-22T06:16:11Z
file_id: '17286'
file_name: 2024_Nature_Gaertner.pdf
file_size: 15704819
relation: main_file
success: 1
file_date_updated: 2024-07-22T06:16:11Z
has_accepted_license: '1'
intvolume: ' 631'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 645-653
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '747687'
name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/heiniglab/gaertner_megakaryocytes
scopus_import: '1'
status: public
title: Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 631
year: '2024'
...