--- _id: '13376' abstract: - lang: eng text: Efficient molecular switching in confined spaces is critical for the successful development of artificial molecular machines. However, molecular switching events often entail large structural changes and therefore require conformational freedom, which is typically limited under confinement conditions. Here, we investigated the behavior of azobenzene—the key building block of light-controlled molecular machines—in a confined environment that is flexible and can adapt its shape to that of the bound guest. To this end, we encapsulated several structurally diverse azobenzenes within the cavity of a flexible, water-soluble coordination cage, and investigated their light-responsive behavior. Using UV/Vis absorption spectroscopy and a combination of NMR methods, we showed that each of the encapsulated azobenzenes exhibited distinct switching properties. An azobenzene forming a 1:1 host–guest inclusion complex could be efficiently photoisomerized in a reversible fashion. In contrast, successful switching in inclusion complexes incorporating two azobenzene guests was dependent on the availability of free cages in the system, and it involved reversible trafficking of azobenzene between the cages. In the absence of extra cages, photoswitching was either suppressed or it involved expulsion of azobenzene from the cage and consequently its precipitation from the solution. This finding was utilized to develop an information storage medium in which messages could be written and erased in a reversible fashion using light. article_processing_charge: No article_type: original author: - first_name: Dipak full_name: Samanta, Dipak last_name: Samanta - first_name: Julius full_name: Gemen, Julius last_name: Gemen - first_name: Zonglin full_name: Chu, Zonglin last_name: Chu - first_name: Yael full_name: Diskin-Posner, Yael last_name: Diskin-Posner - first_name: Linda J. W. full_name: Shimon, Linda J. W. last_name: Shimon - first_name: Rafal full_name: Klajn, Rafal id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b last_name: Klajn citation: ama: Samanta D, Gemen J, Chu Z, Diskin-Posner Y, Shimon LJW, Klajn R. Reversible photoswitching of encapsulated azobenzenes in water. Proceedings of the National Academy of Sciences. 2018;115(38):9379-9384. doi:10.1073/pnas.1712787115 apa: Samanta, D., Gemen, J., Chu, Z., Diskin-Posner, Y., Shimon, L. J. W., & Klajn, R. (2018). Reversible photoswitching of encapsulated azobenzenes in water. Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1712787115 chicago: Samanta, Dipak, Julius Gemen, Zonglin Chu, Yael Diskin-Posner, Linda J. W. Shimon, and Rafal Klajn. “Reversible Photoswitching of Encapsulated Azobenzenes in Water.” Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1712787115. ieee: D. Samanta, J. Gemen, Z. Chu, Y. Diskin-Posner, L. J. W. Shimon, and R. Klajn, “Reversible photoswitching of encapsulated azobenzenes in water,” Proceedings of the National Academy of Sciences, vol. 115, no. 38. Proceedings of the National Academy of Sciences, pp. 9379–9384, 2018. ista: Samanta D, Gemen J, Chu Z, Diskin-Posner Y, Shimon LJW, Klajn R. 2018. Reversible photoswitching of encapsulated azobenzenes in water. Proceedings of the National Academy of Sciences. 115(38), 9379–9384. mla: Samanta, Dipak, et al. “Reversible Photoswitching of Encapsulated Azobenzenes in Water.” Proceedings of the National Academy of Sciences, vol. 115, no. 38, Proceedings of the National Academy of Sciences, 2018, pp. 9379–84, doi:10.1073/pnas.1712787115. short: D. Samanta, J. Gemen, Z. Chu, Y. Diskin-Posner, L.J.W. Shimon, R. Klajn, Proceedings of the National Academy of Sciences 115 (2018) 9379–9384. date_created: 2023-08-01T09:40:00Z date_published: 2018-05-01T00:00:00Z date_updated: 2024-10-14T12:14:53Z day: '01' doi: 10.1073/pnas.1712787115 extern: '1' external_id: pmid: - '29717041' intvolume: ' 115' issue: '38' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1712787115 month: '05' oa: 1 oa_version: Published Version page: 9379-9384 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Reversible photoswitching of encapsulated azobenzenes in water type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 115 year: '2018' ... --- _id: '6010' abstract: - lang: eng text: The optic tectum (TeO), or superior colliculus, is a multisensory midbrain center that organizes spatially orienting responses to relevant stimuli. To define the stimulus with the highest priority at each moment, a network of reciprocal connections between the TeO and the isthmi promotes competition between concurrent tectal inputs. In the avian midbrain, the neurons mediating enhancement and suppression of tectal inputs are located in separate isthmic nuclei, facilitating the analysis of the neural processes that mediate competition. A specific subset of radial neurons in the intermediate tectal layers relay retinal inputs to the isthmi, but at present it is unclear whether separate neurons innervate individual nuclei or a single neural type sends a common input to several of them. In this study, we used in vitro neural tracing and cell-filling experiments in chickens to show that single neurons innervate, via axon collaterals, the three nuclei that comprise the isthmotectal network. This demonstrates that the input signals representing the strength of the incoming stimuli are simultaneously relayed to the mechanisms promoting both enhancement and suppression of the input signals. By performing in vivo recordings in anesthetized chicks, we also show that this common input generates synchrony between both antagonistic mechanisms, demonstrating that activity enhancement and suppression are closely coordinated. From a computational point of view, these results suggest that these tectal neurons constitute integrative nodes that combine inputs from different sources to drive in parallel several concurrent neural processes, each performing complementary functions within the network through different firing patterns and connectivity. article_processing_charge: No author: - first_name: Florencia full_name: Garrido-Charad, Florencia last_name: Garrido-Charad - first_name: Tomas A full_name: Vega Zuniga, Tomas A id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87 last_name: Vega Zuniga - first_name: Cristián full_name: Gutiérrez-Ibáñez, Cristián last_name: Gutiérrez-Ibáñez - first_name: Pedro full_name: Fernandez, Pedro last_name: Fernandez - first_name: Luciana full_name: López-Jury, Luciana last_name: López-Jury - first_name: Cristian full_name: González-Cabrera, Cristian last_name: González-Cabrera - first_name: Harvey J. full_name: Karten, Harvey J. last_name: Karten - first_name: Harald full_name: Luksch, Harald last_name: Luksch - first_name: Gonzalo J. full_name: Marín, Gonzalo J. last_name: Marín citation: ama: Garrido-Charad F, Vega Zuniga TA, Gutiérrez-Ibáñez C, et al. “Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network. Proceedings of the National Academy of Sciences. 2018;115(32):E7615-E7623. doi:10.1073/pnas.1804517115 apa: Garrido-Charad, F., Vega Zuniga, T. A., Gutiérrez-Ibáñez, C., Fernandez, P., López-Jury, L., González-Cabrera, C., … Marín, G. J. (2018). “Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1804517115 chicago: Garrido-Charad, Florencia, Tomas A Vega Zuniga, Cristián Gutiérrez-Ibáñez, Pedro Fernandez, Luciana López-Jury, Cristian González-Cabrera, Harvey J. Karten, Harald Luksch, and Gonzalo J. Marín. ““Shepherd’s Crook” Neurons Drive and Synchronize the Enhancing and Suppressive Mechanisms of the Midbrain Stimulus Selection Network.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1804517115. ieee: F. Garrido-Charad et al., ““Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network,” Proceedings of the National Academy of Sciences, vol. 115, no. 32. National Academy of Sciences, pp. E7615–E7623, 2018. ista: Garrido-Charad F, Vega Zuniga TA, Gutiérrez-Ibáñez C, Fernandez P, López-Jury L, González-Cabrera C, Karten HJ, Luksch H, Marín GJ. 2018. “Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network. Proceedings of the National Academy of Sciences. 115(32), E7615–E7623. mla: Garrido-Charad, Florencia, et al. ““Shepherd’s Crook” Neurons Drive and Synchronize the Enhancing and Suppressive Mechanisms of the Midbrain Stimulus Selection Network.” Proceedings of the National Academy of Sciences, vol. 115, no. 32, National Academy of Sciences, 2018, pp. E7615–23, doi:10.1073/pnas.1804517115. short: F. Garrido-Charad, T.A. Vega Zuniga, C. Gutiérrez-Ibáñez, P. Fernandez, L. López-Jury, C. González-Cabrera, H.J. Karten, H. Luksch, G.J. Marín, Proceedings of the National Academy of Sciences 115 (2018) E7615–E7623. date_created: 2019-02-14T14:33:34Z date_published: 2018-08-07T00:00:00Z date_updated: 2023-09-19T14:35:36Z day: '07' department: - _id: MaJö doi: 10.1073/pnas.1804517115 external_id: isi: - '000440982000020' pmid: - '30026198' intvolume: ' 115' isi: 1 issue: '32' language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pubmed/30026198 month: '08' oa: 1 oa_version: Submitted Version page: E7615-E7623 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: “Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network type: journal_article user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1 volume: 115 year: '2018' ... --- OA_place: repository OA_type: green _id: '19819' abstract: - lang: eng text: The ability to modulate the collective properties of correlated electron systems at their interfaces and surfaces underpins the burgeoning field of “designer” quantum materials. Here, we show how an electronic reconstruction driven by surface polarity mediates a Stoner-like magnetic instability to itinerant ferromagnetism at the Pd-terminated surface of the nonmagnetic delafossite oxide metal PdCoO2. Combining angle-resolved photoemission spectroscopy and density-functional theory calculations, we show how this leads to a rich multiband surface electronic structure. We find similar surface state dispersions in PdCrO2, suggesting surface ferromagnetism persists in this sister compound despite its bulk antiferromagnetic order. article_processing_charge: No article_type: original arxiv: 1 author: - first_name: Federico full_name: Mazzola, Federico last_name: Mazzola - first_name: Veronika full_name: Sunko, Veronika id: 23cb1cf6-2c7a-11ef-91a4-f72fc19f20b3 last_name: Sunko orcid: 0000-0003-2724-3523 - first_name: Seunghyun full_name: Khim, Seunghyun last_name: Khim - first_name: Helge full_name: Rosner, Helge last_name: Rosner - first_name: Pallavi full_name: Kushwaha, Pallavi last_name: Kushwaha - first_name: Oliver J. full_name: Clark, Oliver J. last_name: Clark - first_name: Lewis full_name: Bawden, Lewis last_name: Bawden - first_name: Igor full_name: Marković, Igor last_name: Marković - first_name: Timur K. full_name: Kim, Timur K. last_name: Kim - first_name: Moritz full_name: Hoesch, Moritz last_name: Hoesch - first_name: Andrew P. full_name: Mackenzie, Andrew P. last_name: Mackenzie - first_name: Phil D. C. full_name: King, Phil D. C. last_name: King citation: ama: Mazzola F, Sunko V, Khim S, et al. Itinerant ferromagnetism of the Pd-terminated polar surface of PdCoO2. Proceedings of the National Academy of Sciences. 2018;115(51):12956-12960. doi:10.1073/pnas.1811873115 apa: Mazzola, F., Sunko, V., Khim, S., Rosner, H., Kushwaha, P., Clark, O. J., … King, P. D. C. (2018). Itinerant ferromagnetism of the Pd-terminated polar surface of PdCoO2. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1811873115 chicago: Mazzola, Federico, Veronika Sunko, Seunghyun Khim, Helge Rosner, Pallavi Kushwaha, Oliver J. Clark, Lewis Bawden, et al. “Itinerant Ferromagnetism of the Pd-Terminated Polar Surface of PdCoO2.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1811873115. ieee: F. Mazzola et al., “Itinerant ferromagnetism of the Pd-terminated polar surface of PdCoO2,” Proceedings of the National Academy of Sciences, vol. 115, no. 51. National Academy of Sciences, pp. 12956–12960, 2018. ista: Mazzola F, Sunko V, Khim S, Rosner H, Kushwaha P, Clark OJ, Bawden L, Marković I, Kim TK, Hoesch M, Mackenzie AP, King PDC. 2018. Itinerant ferromagnetism of the Pd-terminated polar surface of PdCoO2. Proceedings of the National Academy of Sciences. 115(51), 12956–12960. mla: Mazzola, Federico, et al. “Itinerant Ferromagnetism of the Pd-Terminated Polar Surface of PdCoO2.” Proceedings of the National Academy of Sciences, vol. 115, no. 51, National Academy of Sciences, 2018, pp. 12956–60, doi:10.1073/pnas.1811873115. short: F. Mazzola, V. Sunko, S. Khim, H. Rosner, P. Kushwaha, O.J. Clark, L. Bawden, I. Marković, T.K. Kim, M. Hoesch, A.P. Mackenzie, P.D.C. King, Proceedings of the National Academy of Sciences 115 (2018) 12956–12960. date_created: 2025-06-10T09:19:14Z date_published: 2018-12-04T00:00:00Z date_updated: 2025-06-10T12:40:00Z day: '04' doi: 10.1073/pnas.1811873115 extern: '1' external_id: arxiv: - '1710.05392' pmid: - '30514820' intvolume: ' 115' issue: '51' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.48550/arXiv.1710.05392 month: '12' oa: 1 oa_version: Preprint page: 12956-12960 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Itinerant ferromagnetism of the Pd-terminated polar surface of PdCoO2 type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 115 year: '2018' ... --- _id: '43' abstract: - lang: eng text: 'The initial amount of pathogens required to start an infection within a susceptible host is called the infective dose and is known to vary to a large extent between different pathogen species. We investigate the hypothesis that the differences in infective doses are explained by the mode of action in the underlying mechanism of pathogenesis: Pathogens with locally acting mechanisms tend to have smaller infective doses than pathogens with distantly acting mechanisms. While empirical evidence tends to support the hypothesis, a formal theoretical explanation has been lacking. We give simple analytical models to gain insight into this phenomenon and also investigate a stochastic, spatially explicit, mechanistic within-host model for toxin-dependent bacterial infections. The model shows that pathogens secreting locally acting toxins have smaller infective doses than pathogens secreting diffusive toxins, as hypothesized. While local pathogenetic mechanisms require smaller infective doses, pathogens with distantly acting toxins tend to spread faster and may cause more damage to the host. The proposed model can serve as a basis for the spatially explicit analysis of various virulence factors also in the context of other problems in infection dynamics.' acknowledgement: J.R. and J.V.A. were also supported by the Academy of Finland Grants 1273253 and 267541. article_processing_charge: No author: - first_name: Joel full_name: Rybicki, Joel id: 334EFD2E-F248-11E8-B48F-1D18A9856A87 last_name: Rybicki orcid: 0000-0002-6432-6646 - first_name: Eva full_name: Kisdi, Eva last_name: Kisdi - first_name: Jani full_name: Anttila, Jani last_name: Anttila citation: ama: Rybicki J, Kisdi E, Anttila J. Model of bacterial toxin-dependent pathogenesis explains infective dose. Proceedings of the National Academy of Sciences of the United States of America. 2018;115(42):10690-10695. doi:10.1073/pnas.1721061115 apa: Rybicki, J., Kisdi, E., & Anttila, J. (2018). Model of bacterial toxin-dependent pathogenesis explains infective dose. Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.1721061115 chicago: Rybicki, Joel, Eva Kisdi, and Jani Anttila. “Model of Bacterial Toxin-Dependent Pathogenesis Explains Infective Dose.” Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1721061115. ieee: J. Rybicki, E. Kisdi, and J. Anttila, “Model of bacterial toxin-dependent pathogenesis explains infective dose,” Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 42. National Academy of Sciences, pp. 10690–10695, 2018. ista: Rybicki J, Kisdi E, Anttila J. 2018. Model of bacterial toxin-dependent pathogenesis explains infective dose. Proceedings of the National Academy of Sciences of the United States of America. 115(42), 10690–10695. mla: Rybicki, Joel, et al. “Model of Bacterial Toxin-Dependent Pathogenesis Explains Infective Dose.” Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 42, National Academy of Sciences, 2018, pp. 10690–95, doi:10.1073/pnas.1721061115. short: J. Rybicki, E. Kisdi, J. Anttila, Proceedings of the National Academy of Sciences of the United States of America 115 (2018) 10690–10695. date_created: 2018-12-11T11:44:19Z date_published: 2018-10-02T00:00:00Z date_updated: 2025-06-03T11:16:28Z day: '02' ddc: - '570' - '577' department: - _id: DaAl doi: 10.1073/pnas.1721061115 ec_funded: 1 external_id: isi: - '000447491300057' file: - access_level: open_access checksum: df7ac544a587c06b75692653b9fabd18 content_type: application/pdf creator: dernst date_created: 2019-04-09T08:02:50Z date_updated: 2020-07-14T12:46:26Z file_id: '6258' file_name: 2018_PNAS_Rybicki.pdf file_size: 4070777 relation: main_file file_date_updated: 2020-07-14T12:46:26Z has_accepted_license: '1' intvolume: ' 115' isi: 1 issue: '42' language: - iso: eng month: '10' oa: 1 oa_version: Submitted Version page: 10690 - 10695 project: - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships publication: Proceedings of the National Academy of Sciences of the United States of America publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences publist_id: '8011' pubrep_id: '1063' quality_controlled: '1' scopus_import: '1' status: public title: Model of bacterial toxin-dependent pathogenesis explains infective dose type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 115 year: '2018' ... --- _id: '9471' abstract: - lang: eng text: The DEMETER (DME) DNA glycosylase catalyzes genome-wide DNA demethylation and is required for endosperm genomic imprinting and embryo viability. Targets of DME-mediated DNA demethylation reside in small, euchromatic, AT-rich transposons and at the boundaries of large transposons, but how DME interacts with these diverse chromatin states is unknown. The STRUCTURE SPECIFIC RECOGNITION PROTEIN 1 (SSRP1) subunit of the chromatin remodeler FACT (facilitates chromatin transactions), was previously shown to be involved in the DME-dependent regulation of genomic imprinting in Arabidopsis endosperm. Therefore, to investigate the interaction between DME and chromatin, we focused on the activity of the two FACT subunits, SSRP1 and SUPPRESSOR of TY16 (SPT16), during reproduction in Arabidopsis. We found that FACT colocalizes with nuclear DME in vivo, and that DME has two classes of target sites, the first being euchromatic and accessible to DME, but the second, representing over half of DME targets, requiring the action of FACT for DME-mediated DNA demethylation genome-wide. Our results show that the FACT-dependent DME targets are GC-rich heterochromatin domains with high nucleosome occupancy enriched with H3K9me2 and H3K27me1. Further, we demonstrate that heterochromatin-associated linker histone H1 specifically mediates the requirement for FACT at a subset of DME-target loci. Overall, our results demonstrate that FACT is required for DME targeting by facilitating its access to heterochromatin. article_processing_charge: No article_type: original author: - first_name: Jennifer M. full_name: Frost, Jennifer M. last_name: Frost - first_name: M. Yvonne full_name: Kim, M. Yvonne last_name: Kim - first_name: Guen Tae full_name: Park, Guen Tae last_name: Park - first_name: Ping-Hung full_name: Hsieh, Ping-Hung last_name: Hsieh - first_name: Miyuki full_name: Nakamura, Miyuki last_name: Nakamura - first_name: Samuel J. H. full_name: Lin, Samuel J. H. last_name: Lin - first_name: Hyunjin full_name: Yoo, Hyunjin last_name: Yoo - first_name: Jaemyung full_name: Choi, Jaemyung last_name: Choi - first_name: Yoko full_name: Ikeda, Yoko last_name: Ikeda - first_name: Tetsu full_name: Kinoshita, Tetsu last_name: Kinoshita - first_name: Yeonhee full_name: Choi, Yeonhee last_name: Choi - first_name: Daniel full_name: Zilberman, Daniel id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1 last_name: Zilberman orcid: 0000-0002-0123-8649 - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer citation: ama: Frost JM, Kim MY, Park GT, et al. FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis. Proceedings of the National Academy of Sciences. 2018;115(20):E4720-E4729. doi:10.1073/pnas.1713333115 apa: Frost, J. M., Kim, M. Y., Park, G. T., Hsieh, P.-H., Nakamura, M., Lin, S. J. H., … Fischer, R. L. (2018). FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1713333115 chicago: Frost, Jennifer M., M. Yvonne Kim, Guen Tae Park, Ping-Hung Hsieh, Miyuki Nakamura, Samuel J. H. Lin, Hyunjin Yoo, et al. “FACT Complex Is Required for DNA Demethylation at Heterochromatin during Reproduction in Arabidopsis.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1713333115. ieee: J. M. Frost et al., “FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis,” Proceedings of the National Academy of Sciences, vol. 115, no. 20. National Academy of Sciences, pp. E4720–E4729, 2018. ista: Frost JM, Kim MY, Park GT, Hsieh P-H, Nakamura M, Lin SJH, Yoo H, Choi J, Ikeda Y, Kinoshita T, Choi Y, Zilberman D, Fischer RL. 2018. FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis. Proceedings of the National Academy of Sciences. 115(20), E4720–E4729. mla: Frost, Jennifer M., et al. “FACT Complex Is Required for DNA Demethylation at Heterochromatin during Reproduction in Arabidopsis.” Proceedings of the National Academy of Sciences, vol. 115, no. 20, National Academy of Sciences, 2018, pp. E4720–29, doi:10.1073/pnas.1713333115. short: J.M. Frost, M.Y. Kim, G.T. Park, P.-H. Hsieh, M. Nakamura, S.J.H. Lin, H. Yoo, J. Choi, Y. Ikeda, T. Kinoshita, Y. Choi, D. Zilberman, R.L. Fischer, Proceedings of the National Academy of Sciences 115 (2018) E4720–E4729. date_created: 2021-06-07T06:11:28Z date_published: 2018-05-15T00:00:00Z date_updated: 2021-12-14T07:53:40Z day: '15' ddc: - '580' department: - _id: DaZi doi: 10.1073/pnas.1713333115 extern: '1' external_id: pmid: - '29712855' file: - access_level: open_access checksum: 810260dc0e3cc3033e15c19ad0dc123e content_type: application/pdf creator: asandaue date_created: 2021-06-07T06:16:38Z date_updated: 2021-06-07T06:16:38Z file_id: '9472' file_name: 2018_PNAS_Frost.pdf file_size: 3045260 relation: main_file success: 1 file_date_updated: 2021-06-07T06:16:38Z has_accepted_license: '1' intvolume: ' 115' issue: '20' keyword: - Multidisciplinary language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '05' oa: 1 oa_version: Published Version page: E4720-E4729 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' related_material: link: - relation: earlier_version url: 'https://doi.org/10.1101/187674 ' scopus_import: '1' status: public title: FACT complex is required for DNA demethylation at heterochromatin during reproduction in Arabidopsis tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 115 year: '2018' ... --- _id: '15157' abstract: - lang: eng text: The basic helix–loop–helix PAS domain (bHLH-PAS) transcription factor CLOCK:BMAL1 (brain and muscle Arnt-like protein 1) sits at the core of the mammalian circadian transcription/translation feedback loop. Precise control of CLOCK:BMAL1 activity by coactivators and repressors establishes the ∼24-h periodicity of gene expression. Formation of a repressive complex, defined by the core clock proteins cryptochrome 1 (CRY1):CLOCK:BMAL1, plays an important role controlling the switch from repression to activation each day. Here we show that CRY1 binds directly to the PAS domain core of CLOCK:BMAL1, driven primarily by interaction with the CLOCK PAS-B domain. Integrative modeling and solution X-ray scattering studies unambiguously position a key loop of the CLOCK PAS-B domain in the secondary pocket of CRY1, analogous to the antenna chromophore-binding pocket of photolyase. CRY1 docks onto the transcription factor alongside the PAS domains, extending above the DNA-binding bHLH domain. Single point mutations at the interface on either CRY1 or CLOCK disrupt formation of the ternary complex, highlighting the importance of this interface for direct regulation of CLOCK:BMAL1 activity by CRY1. article_processing_charge: No article_type: original author: - first_name: Alicia Kathleen full_name: Michael, Alicia Kathleen id: 6437c950-2a03-11ee-914d-d6476dd7b75c last_name: Michael - first_name: Jennifer L. full_name: Fribourgh, Jennifer L. last_name: Fribourgh - first_name: Yogarany full_name: Chelliah, Yogarany last_name: Chelliah - first_name: Colby R. full_name: Sandate, Colby R. last_name: Sandate - first_name: Greg L. full_name: Hura, Greg L. last_name: Hura - first_name: Dina full_name: Schneidman-Duhovny, Dina last_name: Schneidman-Duhovny - first_name: Sarvind M. full_name: Tripathi, Sarvind M. last_name: Tripathi - first_name: Joseph S. full_name: Takahashi, Joseph S. last_name: Takahashi - first_name: Carrie L. full_name: Partch, Carrie L. last_name: Partch citation: ama: Michael AK, Fribourgh JL, Chelliah Y, et al. Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1. Proceedings of the National Academy of Sciences. 2017;114(7):1560-1565. doi:10.1073/pnas.1615310114 apa: Michael, A. K., Fribourgh, J. L., Chelliah, Y., Sandate, C. R., Hura, G. L., Schneidman-Duhovny, D., … Partch, C. L. (2017). Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1. Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1615310114 chicago: Michael, Alicia K., Jennifer L. Fribourgh, Yogarany Chelliah, Colby R. Sandate, Greg L. Hura, Dina Schneidman-Duhovny, Sarvind M. Tripathi, Joseph S. Takahashi, and Carrie L. Partch. “Formation of a Repressive Complex in the Mammalian Circadian Clock Is Mediated by the Secondary Pocket of CRY1.” Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1615310114. ieee: A. K. Michael et al., “Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1,” Proceedings of the National Academy of Sciences, vol. 114, no. 7. Proceedings of the National Academy of Sciences, pp. 1560–1565, 2017. ista: Michael AK, Fribourgh JL, Chelliah Y, Sandate CR, Hura GL, Schneidman-Duhovny D, Tripathi SM, Takahashi JS, Partch CL. 2017. Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1. Proceedings of the National Academy of Sciences. 114(7), 1560–1565. mla: Michael, Alicia K., et al. “Formation of a Repressive Complex in the Mammalian Circadian Clock Is Mediated by the Secondary Pocket of CRY1.” Proceedings of the National Academy of Sciences, vol. 114, no. 7, Proceedings of the National Academy of Sciences, 2017, pp. 1560–65, doi:10.1073/pnas.1615310114. short: A.K. Michael, J.L. Fribourgh, Y. Chelliah, C.R. Sandate, G.L. Hura, D. Schneidman-Duhovny, S.M. Tripathi, J.S. Takahashi, C.L. Partch, Proceedings of the National Academy of Sciences 114 (2017) 1560–1565. date_created: 2024-03-21T07:56:50Z date_published: 2017-01-31T00:00:00Z date_updated: 2024-03-25T12:12:23Z day: '31' doi: 10.1073/pnas.1615310114 extern: '1' external_id: pmid: - '28143926' intvolume: ' 114' issue: '7' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1615310114 month: '01' oa: 1 oa_version: Published Version page: 1560-1565 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1 type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 114 year: '2017' ... --- _id: '10373' abstract: - lang: eng text: 'Electric charges are conserved. The same would be expected to hold for magnetic charges, yet magnetic monopoles have never been observed. It is therefore surprising that the laws of nonequilibrium thermodynamics, combined with Maxwell’s equations, suggest that colloidal particles heated or cooled in certain polar or paramagnetic solvents may behave as if they carry an electric/magnetic charge. Here, we present numerical simulations that show that the field distribution around a pair of such heated/cooled colloidal particles agrees quantitatively with the theoretical predictions for a pair of oppositely charged electric or magnetic monopoles. However, in other respects, the nonequilibrium colloidal particles do not behave as monopoles: They cannot be moved by a homogeneous applied field. The numerical evidence for the monopole-like fields around heated/cooled colloidal particles is crucial because the experimental and numerical determination of forces between such colloidal particles would be complicated by the presence of other effects, such as thermophoresis.' acknowledgement: P.W. acknowledges many invaluable discussions with Martin Neumann, Chao Zhang, Michiel Sprik, Aleks Reinhardt, Carl Pölking, and Tine Curk. We acknowledge financial support from the Austrian Academy of Sciences through a doctoral (DOC) fellowship (to P.W.), the Austrian Science Fund (FWF) within the Spezialforschungsbereich Vienna Computational Materials Laboratory (Project F41) (C.D.), and the European Union Early Training Network NANOTRANS (Grant 674979 to D. Frenkel). The results presented here have been achieved in part using the Vienna Scientific Cluster. article_processing_charge: No article_type: original arxiv: 1 author: - first_name: Peter full_name: Wirnsberger, Peter last_name: Wirnsberger - first_name: Domagoj full_name: Fijan, Domagoj last_name: Fijan - first_name: Roger A. full_name: Lightwood, Roger A. last_name: Lightwood - first_name: Anđela full_name: Šarić, Anđela id: bf63d406-f056-11eb-b41d-f263a6566d8b last_name: Šarić orcid: 0000-0002-7854-2139 - first_name: Christoph full_name: Dellago, Christoph last_name: Dellago - first_name: Daan full_name: Frenkel, Daan last_name: Frenkel citation: ama: Wirnsberger P, Fijan D, Lightwood RA, Šarić A, Dellago C, Frenkel D. Numerical evidence for thermally induced monopoles. Proceedings of the National Academy of Sciences. 2017;114(19):4911-4914. doi:10.1073/pnas.1621494114 apa: Wirnsberger, P., Fijan, D., Lightwood, R. A., Šarić, A., Dellago, C., & Frenkel, D. (2017). Numerical evidence for thermally induced monopoles. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1621494114 chicago: Wirnsberger, Peter, Domagoj Fijan, Roger A. Lightwood, Anđela Šarić, Christoph Dellago, and Daan Frenkel. “Numerical Evidence for Thermally Induced Monopoles.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1621494114. ieee: P. Wirnsberger, D. Fijan, R. A. Lightwood, A. Šarić, C. Dellago, and D. Frenkel, “Numerical evidence for thermally induced monopoles,” Proceedings of the National Academy of Sciences, vol. 114, no. 19. National Academy of Sciences, pp. 4911–4914, 2017. ista: Wirnsberger P, Fijan D, Lightwood RA, Šarić A, Dellago C, Frenkel D. 2017. Numerical evidence for thermally induced monopoles. Proceedings of the National Academy of Sciences. 114(19), 4911–4914. mla: Wirnsberger, Peter, et al. “Numerical Evidence for Thermally Induced Monopoles.” Proceedings of the National Academy of Sciences, vol. 114, no. 19, National Academy of Sciences, 2017, pp. 4911–14, doi:10.1073/pnas.1621494114. short: P. Wirnsberger, D. Fijan, R.A. Lightwood, A. Šarić, C. Dellago, D. Frenkel, Proceedings of the National Academy of Sciences 114 (2017) 4911–4914. date_created: 2021-11-29T09:28:24Z date_published: 2017-04-24T00:00:00Z date_updated: 2021-11-29T09:59:12Z day: '24' doi: 10.1073/pnas.1621494114 extern: '1' external_id: arxiv: - '1610.06840' pmid: - '28439003' intvolume: ' 114' issue: '19' keyword: - multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://www.pnas.org/content/114/19/4911 month: '04' oa: 1 oa_version: Published Version page: 4911-4914 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Numerical evidence for thermally induced monopoles type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 114 year: '2017' ... --- _id: '8018' abstract: - lang: eng text: 'Nervous systems use excitatory cell assemblies to encode and represent sensory percepts. Similarly, synaptically connected cell assemblies or "engrams" are thought to represent memories of past experience. Multiple lines of recent evidence indicate that brain systems create and use inhibitory replicas of excitatory representations for important cognitive functions. Such matched "inhibitory engrams" can form through homeostatic potentiation of inhibition onto postsynaptic cells that show increased levels of excitation. Inhibitory engrams can reduce behavioral responses to familiar stimuli, thereby resulting in behavioral habituation. In addition, by preventing inappropriate activation of excitatory memory engrams, inhibitory engrams can make memories quiescent, stored in a latent form that is available for context-relevant activation. In neural networks with balanced excitatory and inhibitory engrams, the release of innate responses and recall of associative memories can occur through focused disinhibition. Understanding mechanisms that regulate the formation and expression of inhibitory engrams in vivo may help not only to explain key features of cognition but also to provide insight into transdiagnostic traits associated with psychiatric conditions such as autism, schizophrenia, and posttraumatic stress disorder. ' article_processing_charge: No article_type: original author: - first_name: Helen C. full_name: Barron, Helen C. last_name: Barron - first_name: Tim P full_name: Vogels, Tim P id: CB6FF8D2-008F-11EA-8E08-2637E6697425 last_name: Vogels orcid: 0000-0003-3295-6181 - first_name: Timothy E. full_name: Behrens, Timothy E. last_name: Behrens - first_name: Mani full_name: Ramaswami, Mani last_name: Ramaswami citation: ama: Barron HC, Vogels TP, Behrens TE, Ramaswami M. Inhibitory engrams in perception and memory. Proceedings of the National Academy of Sciences. 2017;114(26):6666-6674. doi:10.1073/pnas.1701812114 apa: Barron, H. C., Vogels, T. P., Behrens, T. E., & Ramaswami, M. (2017). Inhibitory engrams in perception and memory. Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1701812114 chicago: Barron, Helen C., Tim P Vogels, Timothy E. Behrens, and Mani Ramaswami. “Inhibitory Engrams in Perception and Memory.” Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1701812114. ieee: H. C. Barron, T. P. Vogels, T. E. Behrens, and M. Ramaswami, “Inhibitory engrams in perception and memory,” Proceedings of the National Academy of Sciences, vol. 114, no. 26. Proceedings of the National Academy of Sciences, pp. 6666–6674, 2017. ista: Barron HC, Vogels TP, Behrens TE, Ramaswami M. 2017. Inhibitory engrams in perception and memory. Proceedings of the National Academy of Sciences. 114(26), 6666–6674. mla: Barron, Helen C., et al. “Inhibitory Engrams in Perception and Memory.” Proceedings of the National Academy of Sciences, vol. 114, no. 26, Proceedings of the National Academy of Sciences, 2017, pp. 6666–74, doi:10.1073/pnas.1701812114. short: H.C. Barron, T.P. Vogels, T.E. Behrens, M. Ramaswami, Proceedings of the National Academy of Sciences 114 (2017) 6666–6674. date_created: 2020-06-25T12:56:58Z date_published: 2017-06-27T00:00:00Z date_updated: 2021-01-12T08:16:33Z day: '27' doi: 10.1073/pnas.1701812114 extern: '1' external_id: pmid: - '28611219' intvolume: ' 114' issue: '26' language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495250/ month: '06' oa: 1 oa_version: Published Version page: 6666-6674 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' status: public title: Inhibitory engrams in perception and memory type: journal_article user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425 volume: 114 year: '2017' ... --- _id: '12618' abstract: - lang: eng text: Mountain ranges are the world’s natural water towers and provide water resources for millions of people. However, their hydrological balance and possible future changes in river flow remain poorly understood because of high meteorological variability, physical inaccessibility, and the complex interplay between climate, cryosphere, and hydrological processes. Here, we use a state-of-the art glacio-hydrological model informed by data from high-altitude observations and the latest climate change scenarios to quantify the climate change impact on water resources of two contrasting catchments vulnerable to changes in the cryosphere. The two study catchments are located in the Central Andes of Chile and in the Nepalese Himalaya in close vicinity of densely populated areas. Although both sites reveal a strong decrease in glacier area, they show a remarkably different hydrological response to projected climate change. In the Juncal catchment in Chile, runoff is likely to sharply decrease in the future and the runoff seasonality is sensitive to projected climatic changes. In the Langtang catchment in Nepal, future water availability is on the rise for decades to come with limited shifts between seasons. Owing to the high spatiotemporal resolution of the simulations and process complexity included in the modeling, the response times and the mechanisms underlying the variations in glacier area and river flow can be well constrained. The projections indicate that climate change adaptation in Central Chile should focus on dealing with a reduction in water availability, whereas in Nepal preparedness for flood extremes should be the policy priority. article_processing_charge: No article_type: original author: - first_name: Silvan full_name: Ragettli, Silvan last_name: Ragettli - first_name: Walter W. full_name: Immerzeel, Walter W. last_name: Immerzeel - first_name: Francesca full_name: Pellicciotti, Francesca id: b28f055a-81ea-11ed-b70c-a9fe7f7b0e70 last_name: Pellicciotti citation: ama: Ragettli S, Immerzeel WW, Pellicciotti F. Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains. PNAS. 2016;113(33):9222-9227. doi:10.1073/pnas.1606526113 apa: Ragettli, S., Immerzeel, W. W., & Pellicciotti, F. (2016). Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains. PNAS. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1606526113 chicago: Ragettli, Silvan, Walter W. Immerzeel, and Francesca Pellicciotti. “Contrasting Climate Change Impact on River Flows from High-Altitude Catchments in the Himalayan and Andes Mountains.” PNAS. Proceedings of the National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1606526113. ieee: S. Ragettli, W. W. Immerzeel, and F. Pellicciotti, “Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains,” PNAS, vol. 113, no. 33. Proceedings of the National Academy of Sciences, pp. 9222–9227, 2016. ista: Ragettli S, Immerzeel WW, Pellicciotti F. 2016. Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains. PNAS. 113(33), 9222–9227. mla: Ragettli, Silvan, et al. “Contrasting Climate Change Impact on River Flows from High-Altitude Catchments in the Himalayan and Andes Mountains.” PNAS, vol. 113, no. 33, Proceedings of the National Academy of Sciences, 2016, pp. 9222–27, doi:10.1073/pnas.1606526113. short: S. Ragettli, W.W. Immerzeel, F. Pellicciotti, PNAS 113 (2016) 9222–9227. date_created: 2023-02-20T08:14:58Z date_published: 2016-08-01T00:00:00Z date_updated: 2023-02-24T10:48:43Z day: '01' doi: 10.1073/pnas.1606526113 extern: '1' external_id: pmid: - '27482082' intvolume: ' 113' issue: '33' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1606526113 month: '08' oa: 1 oa_version: Published Version page: 9222-9227 pmid: 1 publication: PNAS publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 113 year: '2016' ... --- _id: '14304' abstract: - lang: eng text: Despite the recent rapid progress in cryo-electron microscopy (cryo-EM), there still exist ample opportunities for improvement in sample preparation. Macromolecular complexes may disassociate or adopt nonrandom orientations against the extended air–water interface that exists for a short time before the sample is frozen. We designed a hollow support structure using 3D DNA origami to protect complexes from the detrimental effects of cryo-EM sample preparation. For a first proof-of-principle, we concentrated on the transcription factor p53, which binds to specific DNA sequences on double-stranded DNA. The support structures spontaneously form monolayers of preoriented particles in a thin film of water, and offer advantages in particle picking and sorting. By controlling the position of the binding sequence on a single helix that spans the hollow support structure, we also sought to control the orientation of individual p53 complexes. Although the latter did not yet yield the desired results, the support structures did provide partial information about the relative orientations of individual p53 complexes. We used this information to calculate a tomographic 3D reconstruction, and refined this structure to a final resolution of ∼15 Å. This structure settles an ongoing debate about the symmetry of the p53 tetramer bound to DNA. article_processing_charge: No article_type: original author: - first_name: Thomas G. full_name: Martin, Thomas G. last_name: Martin - first_name: Tanmay A. M. full_name: Bharat, Tanmay A. M. last_name: Bharat - first_name: Andreas C. full_name: Joerger, Andreas C. last_name: Joerger - first_name: Xiao-chen full_name: Bai, Xiao-chen last_name: Bai - first_name: Florian M full_name: Praetorius, Florian M id: dfec9381-4341-11ee-8fd8-faa02bba7d62 last_name: Praetorius - first_name: Alan R. full_name: Fersht, Alan R. last_name: Fersht - first_name: Hendrik full_name: Dietz, Hendrik last_name: Dietz - first_name: Sjors H. W. full_name: Scheres, Sjors H. W. last_name: Scheres citation: ama: Martin TG, Bharat TAM, Joerger AC, et al. Design of a molecular support for cryo-EM structure determination. PNAS. 2016;113(47):E7456-E7463. doi:10.1073/pnas.1612720113 apa: Martin, T. G., Bharat, T. A. M., Joerger, A. C., Bai, X., Praetorius, F. M., Fersht, A. R., … Scheres, S. H. W. (2016). Design of a molecular support for cryo-EM structure determination. PNAS. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1612720113 chicago: Martin, Thomas G., Tanmay A. M. Bharat, Andreas C. Joerger, Xiao-chen Bai, Florian M Praetorius, Alan R. Fersht, Hendrik Dietz, and Sjors H. W. Scheres. “Design of a Molecular Support for Cryo-EM Structure Determination.” PNAS. Proceedings of the National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1612720113. ieee: T. G. Martin et al., “Design of a molecular support for cryo-EM structure determination,” PNAS, vol. 113, no. 47. Proceedings of the National Academy of Sciences, pp. E7456–E7463, 2016. ista: Martin TG, Bharat TAM, Joerger AC, Bai X, Praetorius FM, Fersht AR, Dietz H, Scheres SHW. 2016. Design of a molecular support for cryo-EM structure determination. PNAS. 113(47), E7456–E7463. mla: Martin, Thomas G., et al. “Design of a Molecular Support for Cryo-EM Structure Determination.” PNAS, vol. 113, no. 47, Proceedings of the National Academy of Sciences, 2016, pp. E7456–63, doi:10.1073/pnas.1612720113. short: T.G. Martin, T.A.M. Bharat, A.C. Joerger, X. Bai, F.M. Praetorius, A.R. Fersht, H. Dietz, S.H.W. Scheres, PNAS 113 (2016) E7456–E7463. date_created: 2023-09-06T12:53:48Z date_published: 2016-10-13T00:00:00Z date_updated: 2023-11-07T11:53:06Z day: '13' doi: 10.1073/pnas.1612720113 extern: '1' external_id: pmid: - '27821763' intvolume: ' 113' issue: '47' language: - iso: eng month: '10' oa_version: Published Version page: E7456-E7463 pmid: 1 publication: PNAS publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Design of a molecular support for cryo-EM structure determination type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 113 year: '2016' ... --- _id: '9473' abstract: - lang: eng text: Cytosine DNA methylation regulates the expression of eukaryotic genes and transposons. Methylation is copied by methyltransferases after DNA replication, which results in faithful transmission of methylation patterns during cell division and, at least in flowering plants, across generations. Transgenerational inheritance is mediated by a small group of cells that includes gametes and their progenitors. However, methylation is usually analyzed in somatic tissues that do not contribute to the next generation, and the mechanisms of transgenerational inheritance are inferred from such studies. To gain a better understanding of how DNA methylation is inherited, we analyzed purified Arabidopsis thaliana sperm and vegetative cells-the cell types that comprise pollen-with mutations in the DRM, CMT2, and CMT3 methyltransferases. We find that DNA methylation dependency on these enzymes is similar in sperm, vegetative cells, and somatic tissues, although DRM activity extends into heterochromatin in vegetative cells, likely reflecting transcription of heterochromatic transposons in this cell type. We also show that lack of histone H1, which elevates heterochromatic DNA methylation in somatic tissues, does not have this effect in pollen. Instead, levels of CG methylation in wild-type sperm and vegetative cells, as well as in wild-type microspores from which both pollen cell types originate, are substantially higher than in wild-type somatic tissues and similar to those of H1-depleted roots. Our results demonstrate that the mechanisms of methylation maintenance are similar between pollen and somatic cells, but the efficiency of CG methylation is higher in pollen, allowing methylation patterns to be accurately inherited across generations. article_processing_charge: No article_type: original author: - first_name: Ping-Hung full_name: Hsieh, Ping-Hung last_name: Hsieh - first_name: Shengbo full_name: He, Shengbo last_name: He - first_name: Toby full_name: Buttress, Toby last_name: Buttress - first_name: Hongbo full_name: Gao, Hongbo last_name: Gao - first_name: Matthew full_name: Couchman, Matthew last_name: Couchman - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer - first_name: Daniel full_name: Zilberman, Daniel id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1 last_name: Zilberman orcid: 0000-0002-0123-8649 - first_name: Xiaoqi full_name: Feng, Xiaoqi id: e0164712-22ee-11ed-b12a-d80fcdf35958 last_name: Feng orcid: 0000-0002-4008-1234 citation: ama: Hsieh P-H, He S, Buttress T, et al. Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation than somatic tissues. Proceedings of the National Academy of Sciences. 2016;113(52):15132-15137. doi:10.1073/pnas.1619074114 apa: Hsieh, P.-H., He, S., Buttress, T., Gao, H., Couchman, M., Fischer, R. L., … Feng, X. (2016). Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation than somatic tissues. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1619074114 chicago: Hsieh, Ping-Hung, Shengbo He, Toby Buttress, Hongbo Gao, Matthew Couchman, Robert L. Fischer, Daniel Zilberman, and Xiaoqi Feng. “Arabidopsis Male Sexual Lineage Exhibits More Robust Maintenance of CG Methylation than Somatic Tissues.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1619074114. ieee: P.-H. Hsieh et al., “Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation than somatic tissues,” Proceedings of the National Academy of Sciences, vol. 113, no. 52. National Academy of Sciences, pp. 15132–15137, 2016. ista: Hsieh P-H, He S, Buttress T, Gao H, Couchman M, Fischer RL, Zilberman D, Feng X. 2016. Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation than somatic tissues. Proceedings of the National Academy of Sciences. 113(52), 15132–15137. mla: Hsieh, Ping-Hung, et al. “Arabidopsis Male Sexual Lineage Exhibits More Robust Maintenance of CG Methylation than Somatic Tissues.” Proceedings of the National Academy of Sciences, vol. 113, no. 52, National Academy of Sciences, 2016, pp. 15132–37, doi:10.1073/pnas.1619074114. short: P.-H. Hsieh, S. He, T. Buttress, H. Gao, M. Couchman, R.L. Fischer, D. Zilberman, X. Feng, Proceedings of the National Academy of Sciences 113 (2016) 15132–15137. date_created: 2021-06-07T06:21:39Z date_published: 2016-12-27T00:00:00Z date_updated: 2023-05-08T11:00:40Z day: '27' department: - _id: DaZi - _id: XiFe doi: 10.1073/pnas.1619074114 extern: '1' external_id: pmid: - '27956643' intvolume: ' 113' issue: '52' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1619074114 month: '12' oa: 1 oa_version: Published Version page: 15132-15137 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Arabidopsis male sexual lineage exhibits more robust maintenance of CG methylation than somatic tissues type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 113 year: '2016' ... --- _id: '9477' abstract: - lang: eng text: Cytosine methylation is a DNA modification with important regulatory functions in eukaryotes. In flowering plants, sexual reproduction is accompanied by extensive DNA demethylation, which is required for proper gene expression in the endosperm, a nutritive extraembryonic seed tissue. Endosperm arises from a fusion of a sperm cell carried in the pollen and a female central cell. Endosperm DNA demethylation is observed specifically on the chromosomes inherited from the central cell in Arabidopsis thaliana, rice, and maize, and requires the DEMETER DNA demethylase in Arabidopsis. DEMETER is expressed in the central cell before fertilization, suggesting that endosperm demethylation patterns are inherited from the central cell. Down-regulation of the MET1 DNA methyltransferase has also been proposed to contribute to central cell demethylation. However, with the exception of three maize genes, central cell DNA methylation has not been directly measured, leaving the origin and mechanism of endosperm demethylation uncertain. Here, we report genome-wide analysis of DNA methylation in the central cells of Arabidopsis and rice—species that diverged 150 million years ago—as well as in rice egg cells. We find that DNA demethylation in both species is initiated in central cells, which requires DEMETER in Arabidopsis. However, we do not observe a global reduction of CG methylation that would be indicative of lowered MET1 activity; on the contrary, CG methylation efficiency is elevated in female gametes compared with nonsexual tissues. Our results demonstrate that locus-specific, active DNA demethylation in the central cell is the origin of maternal chromosome hypomethylation in the endosperm. article_processing_charge: No article_type: original author: - first_name: Kyunghyuk full_name: Park, Kyunghyuk last_name: Park - first_name: M. Yvonne full_name: Kim, M. Yvonne last_name: Kim - first_name: Martin full_name: Vickers, Martin last_name: Vickers - first_name: Jin-Sup full_name: Park, Jin-Sup last_name: Park - first_name: Youbong full_name: Hyun, Youbong last_name: Hyun - first_name: Takashi full_name: Okamoto, Takashi last_name: Okamoto - first_name: Daniel full_name: Zilberman, Daniel id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1 last_name: Zilberman orcid: 0000-0002-0123-8649 - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer - first_name: Xiaoqi full_name: Feng, Xiaoqi id: e0164712-22ee-11ed-b12a-d80fcdf35958 last_name: Feng orcid: 0000-0002-4008-1234 - first_name: Yeonhee full_name: Choi, Yeonhee last_name: Choi - first_name: Stefan full_name: Scholten, Stefan last_name: Scholten citation: ama: Park K, Kim MY, Vickers M, et al. DNA demethylation is initiated in the central cells of Arabidopsis and rice. Proceedings of the National Academy of Sciences. 2016;113(52):15138-15143. doi:10.1073/pnas.1619047114 apa: Park, K., Kim, M. Y., Vickers, M., Park, J.-S., Hyun, Y., Okamoto, T., … Scholten, S. (2016). DNA demethylation is initiated in the central cells of Arabidopsis and rice. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1619047114 chicago: Park, Kyunghyuk, M. Yvonne Kim, Martin Vickers, Jin-Sup Park, Youbong Hyun, Takashi Okamoto, Daniel Zilberman, et al. “DNA Demethylation Is Initiated in the Central Cells of Arabidopsis and Rice.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1619047114. ieee: K. Park et al., “DNA demethylation is initiated in the central cells of Arabidopsis and rice,” Proceedings of the National Academy of Sciences, vol. 113, no. 52. National Academy of Sciences, pp. 15138–15143, 2016. ista: Park K, Kim MY, Vickers M, Park J-S, Hyun Y, Okamoto T, Zilberman D, Fischer RL, Feng X, Choi Y, Scholten S. 2016. DNA demethylation is initiated in the central cells of Arabidopsis and rice. Proceedings of the National Academy of Sciences. 113(52), 15138–15143. mla: Park, Kyunghyuk, et al. “DNA Demethylation Is Initiated in the Central Cells of Arabidopsis and Rice.” Proceedings of the National Academy of Sciences, vol. 113, no. 52, National Academy of Sciences, 2016, pp. 15138–43, doi:10.1073/pnas.1619047114. short: K. Park, M.Y. Kim, M. Vickers, J.-S. Park, Y. Hyun, T. Okamoto, D. Zilberman, R.L. Fischer, X. Feng, Y. Choi, S. Scholten, Proceedings of the National Academy of Sciences 113 (2016) 15138–15143. date_created: 2021-06-07T07:10:59Z date_published: 2016-12-27T00:00:00Z date_updated: 2023-05-08T11:00:07Z day: '27' department: - _id: DaZi - _id: XiFe doi: 10.1073/pnas.1619047114 extern: '1' external_id: pmid: - '27956642' intvolume: ' 113' issue: '52' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1619047114 month: '12' oa: 1 oa_version: Published Version page: 15138-15143 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: DNA demethylation is initiated in the central cells of Arabidopsis and rice type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 113 year: '2016' ... --- _id: '18371' abstract: - lang: eng text: We consider the problem of exact and inexact matching of weighted undirected graphs, in which a bijective correspondence is sought to minimize a quadratic weight disagreement. This computationally challenging problem is often relaxed as a convex quadratic program, in which the space of permutations is replaced by the space of doubly stochastic matrices. However, the applicability of such a relaxation is poorly understood. We define a broad class of friendly graphs characterized by an easily verifiable spectral property. We prove that for friendly graphs, the convex relaxation is guaranteed to find the exact isomorphism or certify its inexistence. This result is further extended to approximately isomorphic graphs, for which we develop an explicit bound on the amount of weight disagreement under which the relaxation is guaranteed to find the globally optimal approximate isomorphism. We also show that in many cases, the graph matching problem can be further harmlessly relaxed to a convex quadratic program with only n separable linear equality constraints, which is substantially more efficient than the standard relaxation involving 2n equality and n2 inequality constraints. Finally, we show that our results are still valid for unfriendly graphs if additional information in the form of seeds or attributes is allowed, with the latter satisfying an easy to verify spectral characteristic. article_processing_charge: No article_type: original author: - first_name: Yonathan full_name: Aflalo, Yonathan last_name: Aflalo - first_name: Alexander full_name: Bronstein, Alexander id: 58f3726e-7cba-11ef-ad8b-e6e8cb3904e6 last_name: Bronstein orcid: 0000-0001-9699-8730 - first_name: Ron full_name: Kimmel, Ron last_name: Kimmel citation: ama: Aflalo Y, Bronstein AM, Kimmel R. On convex relaxation of graph isomorphism. Proceedings of the National Academy of Sciences. 2015;112(10):2942-2947. doi:10.1073/pnas.1401651112 apa: Aflalo, Y., Bronstein, A. M., & Kimmel, R. (2015). On convex relaxation of graph isomorphism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1401651112 chicago: Aflalo, Yonathan, Alex M. Bronstein, and Ron Kimmel. “On Convex Relaxation of Graph Isomorphism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1401651112. ieee: Y. Aflalo, A. M. Bronstein, and R. Kimmel, “On convex relaxation of graph isomorphism,” Proceedings of the National Academy of Sciences, vol. 112, no. 10. National Academy of Sciences, pp. 2942–2947, 2015. ista: Aflalo Y, Bronstein AM, Kimmel R. 2015. On convex relaxation of graph isomorphism. Proceedings of the National Academy of Sciences. 112(10), 2942–2947. mla: Aflalo, Yonathan, et al. “On Convex Relaxation of Graph Isomorphism.” Proceedings of the National Academy of Sciences, vol. 112, no. 10, National Academy of Sciences, 2015, pp. 2942–47, doi:10.1073/pnas.1401651112. short: Y. Aflalo, A.M. Bronstein, R. Kimmel, Proceedings of the National Academy of Sciences 112 (2015) 2942–2947. date_created: 2024-10-15T11:20:54Z date_published: 2015-03-10T00:00:00Z date_updated: 2024-11-12T08:54:37Z day: '10' doi: 10.1073/pnas.1401651112 extern: '1' external_id: pmid: - '25713342' intvolume: ' 112' issue: '10' language: - iso: eng month: '03' oa_version: None page: 2942-2947 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: On convex relaxation of graph isomorphism type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 112 year: '2015' ... --- OA_place: publisher OA_type: free access _id: '866' abstract: - lang: eng text: Proteases play important roles in many biologic processes and are key mediators of cancer, inflammation, and thrombosis. However, comprehensive and quantitative techniques to define the substrate specificity profile of proteases are lacking. The metalloprotease ADAMTS13 regulates blood coagulation by cleaving von Willebrand factor (VWF), reducing its procoagulant activity. A mutagenized substrate phage display library based on a 73-amino acid fragment of VWF was constructed, and the ADAMTS13-dependent change in library complexity was evaluated over reaction time points, using high-throughput sequencing. Reaction rate constants (kcat/KM) were calculated for nearly every possible single amino acid substitution within this fragment. This massively parallel enzyme kinetics analysis detailed the specificity of ADAMTS13 and demonstrated the critical importance of the P1-P1' substrate residues while defining exosite binding domains. These data provided empirical evidence for the propensity for epistasis within VWF and showed strong correlation to conservation across orthologs, highlighting evolutionary selective pressures for VWF. acknowledgement: "We thank Isabel Wang and Vivian Cheung from the Life Sciences Institute, University of Michigan, for assistance with high- throughput sequencing experiments and valuable discussions. We also thank J. Evan Sadler (Washington University) and Sriram Krishnaswamy (Children’s Hospital of Philadelphia) for helpful discussions. We thank Jeff Weitz (McMaster University), Jim Fredenburgh (McMaster University), and Steve Weiss (University of Michigan) for critical review of the manuscript. C.A.K. was awarded the Judith Graham Pool Fellowship from National Hemophilia Foundation. This work was supported by the National Institutes of Health (R01 HL039693), the National Heart, Lung, and Blood Institute (P01- HL057346), Ministerio de Economía y Competitividad Grants BFU2012- 31329 and Sev-2012-0208, and European Research Council Starting Grant 335980_EinME. D.G. is an investigator of the Howard Hughes Medical In- stitute, and F.A.K. is a Howard Hughes Medical Institute International Early Career Scientist.\r\n" article_processing_charge: No article_type: original author: - first_name: Colin full_name: Kretz, Colin last_name: Kretz - first_name: Manhong full_name: Dai, Manhong last_name: Dai - first_name: Onuralp full_name: Soylemez, Onuralp last_name: Soylemez - first_name: Andrew full_name: Yee, Andrew last_name: Yee - first_name: Karl full_name: Desch, Karl last_name: Desch - first_name: David full_name: Siemieniak, David last_name: Siemieniak - first_name: Kärt full_name: Tomberg, Kärt last_name: Tomberg - first_name: Fyodor full_name: Kondrashov, Fyodor id: 44FDEF62-F248-11E8-B48F-1D18A9856A87 last_name: Kondrashov orcid: 0000-0001-8243-4694 - first_name: Fan full_name: Meng, Fan last_name: Meng - first_name: David full_name: Ginsburg, David last_name: Ginsburg citation: ama: Kretz C, Dai M, Soylemez O, et al. Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. PNAS. 2015;112(30):9328-9333. doi:10.1073/pnas.1511328112 apa: Kretz, C., Dai, M., Soylemez, O., Yee, A., Desch, K., Siemieniak, D., … Ginsburg, D. (2015). Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1511328112 chicago: Kretz, Colin, Manhong Dai, Onuralp Soylemez, Andrew Yee, Karl Desch, David Siemieniak, Kärt Tomberg, Fyodor Kondrashov, Fan Meng, and David Ginsburg. “Massively Parallel Enzyme Kinetics Reveals the Substrate Recognition Landscape of the Metalloprotease ADAMTS13.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1511328112. ieee: C. Kretz et al., “Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13,” PNAS, vol. 112, no. 30. National Academy of Sciences, pp. 9328–9333, 2015. ista: Kretz C, Dai M, Soylemez O, Yee A, Desch K, Siemieniak D, Tomberg K, Kondrashov F, Meng F, Ginsburg D. 2015. Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. PNAS. 112(30), 9328–9333. mla: Kretz, Colin, et al. “Massively Parallel Enzyme Kinetics Reveals the Substrate Recognition Landscape of the Metalloprotease ADAMTS13.” PNAS, vol. 112, no. 30, National Academy of Sciences, 2015, pp. 9328–33, doi:10.1073/pnas.1511328112. short: C. Kretz, M. Dai, O. Soylemez, A. Yee, K. Desch, D. Siemieniak, K. Tomberg, F. Kondrashov, F. Meng, D. Ginsburg, PNAS 112 (2015) 9328–9333. date_created: 2018-12-11T11:48:55Z date_published: 2015-07-28T00:00:00Z date_updated: 2026-05-19T13:15:29Z day: '28' doi: 10.1073/pnas.1511328112 extern: '1' external_id: pmid: - ' 26170332' intvolume: ' 112' issue: '30' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1511328112 month: '07' oa: 1 oa_version: Accepted Version page: 9328 - 9333 pmid: 1 publication: PNAS publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences publist_id: '6783' quality_controlled: '1' scopus_import: '1' status: public title: Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13 type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 112 year: '2015' ... --- OA_place: publisher OA_type: hybrid _id: '933' abstract: - lang: eng text: Although collective cell motion plays an important role, for example during wound healing, embryogenesis, or cancer progression, the fundamental rules governing this motion are still not well understood, in particular at high cell density. We study here the motion of human bronchial epithelial cells within a monolayer, over long times. We observe that, as the monolayer ages, the cells slow down monotonously, while the velocity correlation length first increases as the cells slow down but eventually decreases at the slowest motions. By comparing experiments, analytic model, and detailed particle-based simulations, we shed light on this biological amorphous solidification process, demonstrating that the observed dynamics can be explained as a consequence of the combined maturation and strengthening of cell-cell and cell-substrate adhesions. Surprisingly, the increase of cell surface density due to proliferation is only secondary in this process. This analysis is confirmed with two other cell types. The very general relations between the mean cell velocity and velocity correlation lengths, which apply for aggregates of self-propelled particles, as well as motile cells, can possibly be used to discriminate between various parameter changes in vivo, from noninvasive microscopy data. article_processing_charge: No article_type: original author: - first_name: Simón full_name: García, Simón last_name: García - first_name: Edouard B full_name: Hannezo, Edouard B id: 3A9DB764-F248-11E8-B48F-1D18A9856A87 last_name: Hannezo orcid: 0000-0001-6005-1561 - first_name: Jens full_name: Elgeti, Jens last_name: Elgeti - first_name: Jean full_name: Joanny, Jean last_name: Joanny - first_name: Pascal full_name: Silberzan, Pascal last_name: Silberzan - first_name: Nir full_name: Gov, Nir last_name: Gov citation: ama: García S, Hannezo EB, Elgeti J, Joanny J, Silberzan P, Gov N. Physics of active jamming during collective cellular motion in a monolayer. PNAS. 2015;112(50):15314-15319. doi:10.1073/pnas.1510973112 apa: García, S., Hannezo, E. B., Elgeti, J., Joanny, J., Silberzan, P., & Gov, N. (2015). Physics of active jamming during collective cellular motion in a monolayer. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1510973112 chicago: García, Simón, Edouard B Hannezo, Jens Elgeti, Jean Joanny, Pascal Silberzan, and Nir Gov. “Physics of Active Jamming during Collective Cellular Motion in a Monolayer.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1510973112. ieee: S. García, E. B. Hannezo, J. Elgeti, J. Joanny, P. Silberzan, and N. Gov, “Physics of active jamming during collective cellular motion in a monolayer,” PNAS, vol. 112, no. 50. National Academy of Sciences, pp. 15314–15319, 2015. ista: García S, Hannezo EB, Elgeti J, Joanny J, Silberzan P, Gov N. 2015. Physics of active jamming during collective cellular motion in a monolayer. PNAS. 112(50), 15314–15319. mla: García, Simón, et al. “Physics of Active Jamming during Collective Cellular Motion in a Monolayer.” PNAS, vol. 112, no. 50, National Academy of Sciences, 2015, pp. 15314–19, doi:10.1073/pnas.1510973112. short: S. García, E.B. Hannezo, J. Elgeti, J. Joanny, P. Silberzan, N. Gov, PNAS 112 (2015) 15314–15319. date_created: 2018-12-11T11:49:16Z date_published: 2015-12-15T00:00:00Z date_updated: 2026-05-19T10:19:15Z day: '15' doi: 10.1073/pnas.1510973112 extern: '1' external_id: pmid: - '26627719' intvolume: ' 112' issue: '50' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1510973112 month: '12' oa: 1 oa_version: Published Version page: 15314 - 15319 pmid: 1 publication: PNAS publication_identifier: eissn: - 1091-6490 issn: - ' 0027-8424' publication_status: published publisher: National Academy of Sciences publist_id: '6511' quality_controlled: '1' scopus_import: '1' status: public title: Physics of active jamming during collective cellular motion in a monolayer type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 112 year: '2015' ... --- _id: '10382' abstract: - lang: eng text: 'Protein oligomers have been implicated as toxic agents in a wide range of amyloid-related diseases. However, it has remained unsolved whether the oligomers are a necessary step in the formation of amyloid fibrils or just a dangerous byproduct. Analogously, it has not been resolved if the amyloid nucleation process is a classical one-step nucleation process or a two-step process involving prenucleation clusters. We use coarse-grained computer simulations to study the effect of nonspecific attractions between peptides on the primary nucleation process underlying amyloid fibrillization. We find that, for peptides that do not attract, the classical one-step nucleation mechanism is possible but only at nonphysiologically high peptide concentrations. At low peptide concentrations, which mimic the physiologically relevant regime, attractive interpeptide interactions are essential for fibril formation. Nucleation then inevitably takes place through a two-step mechanism involving prefibrillar oligomers. We show that oligomers not only help peptides meet each other but also, create an environment that facilitates the conversion of monomers into the β-sheet–rich form characteristic of fibrils. Nucleation typically does not proceed through the most prevalent oligomers but through an oligomer size that is only observed in rare fluctuations, which is why such aggregates might be hard to capture experimentally. Finally, we find that the nucleation of amyloid fibrils cannot be described by classical nucleation theory: in the two-step mechanism, the critical nucleus size increases with increases in both concentration and interpeptide interactions, which is in direct contrast with predictions from classical nucleation theory.' acknowledgement: We thank Michele Vendruscolo, Iskra Staneva, and William M. Jacobs, for helpful discussions. A.Š. acknowledges support from the Human Frontier Science Program and Emmanuel College. Y.C.C. and D.F. are supported by Engineering and Physical Sciences Research Council Programme Grant EP/I001352/1. T.P.J.K. acknowledges the Frances and Augustus Newman Foundation, the European Research Council, and the Biotechnology and Biological Sciences Research Council. D.F. acknowledges European Research Council Advanced Grant 227758. article_processing_charge: No article_type: original arxiv: 1 author: - first_name: Anđela full_name: Šarić, Anđela id: bf63d406-f056-11eb-b41d-f263a6566d8b last_name: Šarić orcid: 0000-0002-7854-2139 - first_name: Yassmine C. full_name: Chebaro, Yassmine C. last_name: Chebaro - first_name: Tuomas P. J. full_name: Knowles, Tuomas P. J. last_name: Knowles - first_name: Daan full_name: Frenkel, Daan last_name: Frenkel citation: ama: Šarić A, Chebaro YC, Knowles TPJ, Frenkel D. Crucial role of nonspecific interactions in amyloid nucleation. Proceedings of the National Academy of Sciences. 2014;111(50):17869-17874. doi:10.1073/pnas.1410159111 apa: Šarić, A., Chebaro, Y. C., Knowles, T. P. J., & Frenkel, D. (2014). Crucial role of nonspecific interactions in amyloid nucleation. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1410159111 chicago: Šarić, Anđela, Yassmine C. Chebaro, Tuomas P. J. Knowles, and Daan Frenkel. “Crucial Role of Nonspecific Interactions in Amyloid Nucleation.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1410159111. ieee: A. Šarić, Y. C. Chebaro, T. P. J. Knowles, and D. Frenkel, “Crucial role of nonspecific interactions in amyloid nucleation,” Proceedings of the National Academy of Sciences, vol. 111, no. 50. National Academy of Sciences, pp. 17869–17874, 2014. ista: Šarić A, Chebaro YC, Knowles TPJ, Frenkel D. 2014. Crucial role of nonspecific interactions in amyloid nucleation. Proceedings of the National Academy of Sciences. 111(50), 17869–17874. mla: Šarić, Anđela, et al. “Crucial Role of Nonspecific Interactions in Amyloid Nucleation.” Proceedings of the National Academy of Sciences, vol. 111, no. 50, National Academy of Sciences, 2014, pp. 17869–74, doi:10.1073/pnas.1410159111. short: A. Šarić, Y.C. Chebaro, T.P.J. Knowles, D. Frenkel, Proceedings of the National Academy of Sciences 111 (2014) 17869–17874. date_created: 2021-11-29T13:09:53Z date_published: 2014-12-01T00:00:00Z date_updated: 2021-11-29T13:29:05Z day: '01' doi: 10.1073/pnas.1410159111 extern: '1' external_id: arxiv: - '1412.0897' pmid: - '25453085' intvolume: ' 111' issue: '50' keyword: - multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://www.pnas.org/content/111/50/17869 month: '12' oa: 1 oa_version: Published Version page: 17869-17874 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Crucial role of nonspecific interactions in amyloid nucleation type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 111 year: '2014' ... --- OA_type: closed access _id: '17990' abstract: - lang: eng text: Molecular junctions formed using the scanning-tunneling-microscope–based break-junction technique (STM-BJ) have provided unique insight into charge transport at the nanoscale. In most prior work, the same metal, typically Au, Pt, or Ag, is used for both tip and substrate. For such noble metal electrodes, the density of electronic states is approximately constant within a narrow energy window relevant to charge transport. Here, we form molecular junctions using the STM-BJ technique, with an Au metal tip and a microfabricated graphite substrate, and measure the conductance of a series of graphite/amine-terminated oligophenyl/Au molecular junctions. The remarkable mechanical strength of graphite and the single-crystal properties of our substrates allow measurements over few thousand junctions without any change in the surface properties. We show that conductance decays exponentially with molecular backbone length with a decay constant that is essentially the same as that for measurements with two Au electrodes. More importantly, despite the inherent symmetry of the oligophenylamines, we observe rectification in these junctions. State-of-art ab initio conductance calculations are in good agreement with experiment, and explain the rectification. We show that the highly energy-dependent graphite density of states contributes variations in transmission that, when coupled with an asymmetric voltage drop across the junction, leads to the observed rectification. Together, our measurements and calculations show how functionality may emerge from hybrid molecular-scale devices purposefully designed with different electrodes beyond the so-called “wide band limit,” opening up the possibility of assembling molecular junctions with dissimilar electrodes using layered 2D materials. article_processing_charge: No article_type: original author: - first_name: Taekyeong full_name: Kim, Taekyeong last_name: Kim - first_name: Zhen-Fei full_name: Liu, Zhen-Fei last_name: Liu - first_name: Chulho full_name: Lee, Chulho last_name: Lee - first_name: Jeffrey B. full_name: Neaton, Jeffrey B. last_name: Neaton - first_name: Latha full_name: Venkataraman, Latha id: 9ebb78a5-cc0d-11ee-8322-fae086a32caf last_name: Venkataraman orcid: 0000-0002-6957-6089 citation: ama: Kim T, Liu Z-F, Lee C, Neaton JB, Venkataraman L. Charge transport and rectification in molecular junctions formed with carbon-based electrodes. Proceedings of the National Academy of Sciences. 2014;111(30):10928-10932. doi:10.1073/pnas.1406926111 apa: Kim, T., Liu, Z.-F., Lee, C., Neaton, J. B., & Venkataraman, L. (2014). Charge transport and rectification in molecular junctions formed with carbon-based electrodes. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1406926111 chicago: Kim, Taekyeong, Zhen-Fei Liu, Chulho Lee, Jeffrey B. Neaton, and Latha Venkataraman. “Charge Transport and Rectification in Molecular Junctions Formed with Carbon-Based Electrodes.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1406926111. ieee: T. Kim, Z.-F. Liu, C. Lee, J. B. Neaton, and L. Venkataraman, “Charge transport and rectification in molecular junctions formed with carbon-based electrodes,” Proceedings of the National Academy of Sciences, vol. 111, no. 30. National Academy of Sciences, pp. 10928–10932, 2014. ista: Kim T, Liu Z-F, Lee C, Neaton JB, Venkataraman L. 2014. Charge transport and rectification in molecular junctions formed with carbon-based electrodes. Proceedings of the National Academy of Sciences. 111(30), 10928–10932. mla: Kim, Taekyeong, et al. “Charge Transport and Rectification in Molecular Junctions Formed with Carbon-Based Electrodes.” Proceedings of the National Academy of Sciences, vol. 111, no. 30, National Academy of Sciences, 2014, pp. 10928–32, doi:10.1073/pnas.1406926111. short: T. Kim, Z.-F. Liu, C. Lee, J.B. Neaton, L. Venkataraman, Proceedings of the National Academy of Sciences 111 (2014) 10928–10932. date_created: 2024-09-09T11:26:38Z date_published: 2014-07-14T00:00:00Z date_updated: 2025-01-03T07:49:30Z day: '14' doi: 10.1073/pnas.1406926111 extern: '1' external_id: pmid: - '25024198' intvolume: ' 111' issue: '30' language: - iso: eng month: '07' oa_version: None page: 10928-10932 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Charge transport and rectification in molecular junctions formed with carbon-based electrodes type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 111 year: '2014' ... --- _id: '8021' abstract: - lang: eng text: 'Most excitatory inputs in the mammalian brain are made on dendritic spines, rather than on dendritic shafts. Spines compartmentalize calcium, and this biochemical isolation can underlie input-specific synaptic plasticity, providing a raison d''etre for spines. However, recent results indicate that the spine can experience a membrane potential different from that in the parent dendrite, as though the spine neck electrically isolated the spine. Here we use two-photon calcium imaging of mouse neocortical pyramidal neurons to analyze the correlation between the morphologies of spines activated under minimal synaptic stimulation and the excitatory postsynaptic potentials they generate. We find that excitatory postsynaptic potential amplitudes are inversely correlated with spine neck lengths. Furthermore, a spike timing-dependent plasticity protocol, in which two-photon glutamate uncaging over a spine is paired with postsynaptic spikes, produces rapid shrinkage of the spine neck and concomitant increases in the amplitude of the evoked spine potentials. Using numerical simulations, we explore the parameter regimes for the spine neck resistance and synaptic conductance changes necessary to explain our observations. Our data, directly correlating synaptic and morphological plasticity, imply that long-necked spines have small or negligible somatic voltage contributions, but that, upon synaptic stimulation paired with postsynaptic activity, they can shorten their necks and increase synaptic efficacy, thus changing the input/output gain of pyramidal neurons. ' article_processing_charge: No article_type: original author: - first_name: R. full_name: Araya, R. last_name: Araya - first_name: Tim P full_name: Vogels, Tim P id: CB6FF8D2-008F-11EA-8E08-2637E6697425 last_name: Vogels orcid: 0000-0003-3295-6181 - first_name: R. full_name: Yuste, R. last_name: Yuste citation: ama: Araya R, Vogels TP, Yuste R. Activity-dependent dendritic spine neck changes are correlated with synaptic strength. Proceedings of the National Academy of Sciences. 2014;111(28):E2895-E2904. doi:10.1073/pnas.1321869111 apa: Araya, R., Vogels, T. P., & Yuste, R. (2014). Activity-dependent dendritic spine neck changes are correlated with synaptic strength. Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1321869111 chicago: Araya, R., Tim P Vogels, and R. Yuste. “Activity-Dependent Dendritic Spine Neck Changes Are Correlated with Synaptic Strength.” Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1321869111. ieee: R. Araya, T. P. Vogels, and R. Yuste, “Activity-dependent dendritic spine neck changes are correlated with synaptic strength,” Proceedings of the National Academy of Sciences, vol. 111, no. 28. Proceedings of the National Academy of Sciences, pp. E2895–E2904, 2014. ista: Araya R, Vogels TP, Yuste R. 2014. Activity-dependent dendritic spine neck changes are correlated with synaptic strength. Proceedings of the National Academy of Sciences. 111(28), E2895–E2904. mla: Araya, R., et al. “Activity-Dependent Dendritic Spine Neck Changes Are Correlated with Synaptic Strength.” Proceedings of the National Academy of Sciences, vol. 111, no. 28, Proceedings of the National Academy of Sciences, 2014, pp. E2895–904, doi:10.1073/pnas.1321869111. short: R. Araya, T.P. Vogels, R. Yuste, Proceedings of the National Academy of Sciences 111 (2014) E2895–E2904. date_created: 2020-06-25T13:06:24Z date_published: 2014-07-15T00:00:00Z date_updated: 2021-01-12T08:16:34Z day: '15' doi: 10.1073/pnas.1321869111 extern: '1' external_id: pmid: - '24982196' intvolume: ' 111' issue: '28' language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104910/ month: '07' oa: 1 oa_version: Published Version page: E2895-E2904 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: Proceedings of the National Academy of Sciences quality_controlled: '1' status: public title: Activity-dependent dendritic spine neck changes are correlated with synaptic strength type: journal_article user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425 volume: 111 year: '2014' ... --- _id: '9479' abstract: - lang: eng text: Centromeres mediate chromosome segregation and are defined by the centromere-specific histone H3 variant (CenH3)/centromere protein A (CENP-A). Removal of CenH3 from centromeres is a general property of terminally differentiated cells, and the persistence of CenH3 increases the risk of diseases such as cancer. However, active mechanisms of centromere disassembly are unknown. Nondividing Arabidopsis pollen vegetative cells, which transport engulfed sperm by extended tip growth, undergo loss of CenH3; centromeric heterochromatin decondensation; and bulk activation of silent rRNA genes, accompanied by their translocation into the nucleolus. Here, we show that these processes are blocked by mutations in the evolutionarily conserved AAA-ATPase molecular chaperone, CDC48A, homologous to yeast Cdc48 and human p97 proteins, both of which are implicated in ubiquitin/small ubiquitin-like modifier (SUMO)-targeted protein degradation. We demonstrate that CDC48A physically associates with its heterodimeric cofactor UFD1-NPL4, known to bind ubiquitin and SUMO, as well as with SUMO1-modified CenH3 and mutations in NPL4 phenocopy cdc48a mutations. In WT vegetative cell nuclei, genetically unlinked ribosomal DNA (rDNA) loci are uniquely clustered together within the nucleolus and all major rRNA gene variants, including those rDNA variants silenced in leaves, are transcribed. In cdc48a mutant vegetative cell nuclei, however, these rDNA loci frequently colocalized with condensed centromeric heterochromatin at the external periphery of the nucleolus. Our results indicate that the CDC48ANPL4 complex actively removes sumoylated CenH3 from centromeres and disrupts centromeric heterochromatin to release bulk rRNA genes into the nucleolus for ribosome production, which fuels single nucleus-driven pollen tube growth and is essential for plant reproduction. article_processing_charge: No article_type: original author: - first_name: Zsuzsanna full_name: Mérai, Zsuzsanna last_name: Mérai - first_name: Nina full_name: Chumak, Nina last_name: Chumak - first_name: Marcelina full_name: García-Aguilar, Marcelina last_name: García-Aguilar - first_name: Tzung-Fu full_name: Hsieh, Tzung-Fu last_name: Hsieh - first_name: Toshiro full_name: Nishimura, Toshiro last_name: Nishimura - first_name: Vera K. full_name: Schoft, Vera K. last_name: Schoft - first_name: János full_name: Bindics, János last_name: Bindics - first_name: Lucyna full_name: Ślusarz, Lucyna last_name: Ślusarz - first_name: Stéphanie full_name: Arnoux, Stéphanie last_name: Arnoux - first_name: Susanne full_name: Opravil, Susanne last_name: Opravil - first_name: Karl full_name: Mechtler, Karl last_name: Mechtler - first_name: Daniel full_name: Zilberman, Daniel id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1 last_name: Zilberman orcid: 0000-0002-0123-8649 - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer - first_name: Hisashi full_name: Tamaru, Hisashi last_name: Tamaru citation: ama: Mérai Z, Chumak N, García-Aguilar M, et al. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proceedings of the National Academy of Sciences. 2014;111(45):16166-16171. doi:10.1073/pnas.1418564111 apa: Mérai, Z., Chumak, N., García-Aguilar, M., Hsieh, T.-F., Nishimura, T., Schoft, V. K., … Tamaru, H. (2014). The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1418564111 chicago: Mérai, Zsuzsanna, Nina Chumak, Marcelina García-Aguilar, Tzung-Fu Hsieh, Toshiro Nishimura, Vera K. Schoft, János Bindics, et al. “The AAA-ATPase Molecular Chaperone Cdc48/P97 Disassembles Sumoylated Centromeres, Decondenses Heterochromatin, and Activates Ribosomal RNA Genes.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1418564111. ieee: Z. Mérai et al., “The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes,” Proceedings of the National Academy of Sciences, vol. 111, no. 45. National Academy of Sciences, pp. 16166–16171, 2014. ista: Mérai Z, Chumak N, García-Aguilar M, Hsieh T-F, Nishimura T, Schoft VK, Bindics J, Ślusarz L, Arnoux S, Opravil S, Mechtler K, Zilberman D, Fischer RL, Tamaru H. 2014. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proceedings of the National Academy of Sciences. 111(45), 16166–16171. mla: Mérai, Zsuzsanna, et al. “The AAA-ATPase Molecular Chaperone Cdc48/P97 Disassembles Sumoylated Centromeres, Decondenses Heterochromatin, and Activates Ribosomal RNA Genes.” Proceedings of the National Academy of Sciences, vol. 111, no. 45, National Academy of Sciences, 2014, pp. 16166–71, doi:10.1073/pnas.1418564111. short: Z. Mérai, N. Chumak, M. García-Aguilar, T.-F. Hsieh, T. Nishimura, V.K. Schoft, J. Bindics, L. Ślusarz, S. Arnoux, S. Opravil, K. Mechtler, D. Zilberman, R.L. Fischer, H. Tamaru, Proceedings of the National Academy of Sciences 111 (2014) 16166–16171. date_created: 2021-06-07T07:23:43Z date_published: 2014-11-11T00:00:00Z date_updated: 2021-12-14T08:23:26Z day: '11' department: - _id: DaZi doi: 10.1073/pnas.1418564111 extern: '1' external_id: pmid: - '25344531' intvolume: ' 111' issue: '45' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1418564111 month: '11' oa: 1 oa_version: Published Version page: 16166-16171 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 111 year: '2014' ... --- _id: '9481' abstract: - lang: eng text: Arabidopsis thaliana endosperm, a transient tissue that nourishes the embryo, exhibits extensive localized DNA demethylation on maternally inherited chromosomes. Demethylation mediates parent-of-origin–specific (imprinted) gene expression but is apparently unnecessary for the extensive accumulation of maternally biased small RNA (sRNA) molecules detected in seeds. Endosperm DNA in the distantly related monocots rice and maize is likewise locally hypomethylated, but whether this hypomethylation is generally parent-of-origin specific is unknown. Imprinted expression of sRNA also remains uninvestigated in monocot seeds. Here, we report high-coverage sequencing of the Kitaake rice cultivar that enabled us to show that localized hypomethylation in rice endosperm occurs solely on the maternal genome, preferring regions of high DNA accessibility. Maternally expressed imprinted genes are enriched for hypomethylation at putative promoter regions and transcriptional termini and paternally expressed genes at promoters and gene bodies, mirroring our recent results in A. thaliana. However, unlike in A. thaliana, rice endosperm sRNA populations are dominated by specific strong sRNA-producing loci, and imprinted 24-nt sRNAs are expressed from both parental genomes and correlate with hypomethylation. Overlaps between imprinted sRNA loci and imprinted genes expressed from opposite alleles suggest that sRNAs may regulate genomic imprinting. Whereas sRNAs in seedling tissues primarily originate from small class II (cut-and-paste) transposable elements, those in endosperm are more uniformly derived, including sequences from other transposon classes, as well as genic and intergenic regions. Our data indicate that the endosperm exhibits a unique pattern of sRNA expression and suggest that localized hypomethylation of maternal endosperm DNA is conserved in flowering plants. article_processing_charge: No article_type: original author: - first_name: Jessica A. full_name: Rodrigues, Jessica A. last_name: Rodrigues - first_name: Randy full_name: Ruan, Randy last_name: Ruan - first_name: Toshiro full_name: Nishimura, Toshiro last_name: Nishimura - first_name: Manoj K. full_name: Sharma, Manoj K. last_name: Sharma - first_name: Rita full_name: Sharma, Rita last_name: Sharma - first_name: Pamela C full_name: Ronald, Pamela C last_name: Ronald - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer - first_name: Daniel full_name: Zilberman, Daniel id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1 last_name: Zilberman orcid: 0000-0002-0123-8649 citation: ama: Rodrigues JA, Ruan R, Nishimura T, et al. Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm. Proceedings of the National Academy of Sciences. 2013;110(19):7934-7939. doi:10.1073/pnas.1306164110 apa: Rodrigues, J. A., Ruan, R., Nishimura, T., Sharma, M. K., Sharma, R., Ronald, P. C., … Zilberman, D. (2013). Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.1306164110 chicago: Rodrigues, Jessica A., Randy Ruan, Toshiro Nishimura, Manoj K. Sharma, Rita Sharma, Pamela C Ronald, Robert L. Fischer, and Daniel Zilberman. “Imprinted Expression of Genes and Small RNA Is Associated with Localized Hypomethylation of the Maternal Genome in Rice Endosperm.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1306164110. ieee: J. A. Rodrigues et al., “Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm,” Proceedings of the National Academy of Sciences, vol. 110, no. 19. National Academy of Sciences, pp. 7934–7939, 2013. ista: Rodrigues JA, Ruan R, Nishimura T, Sharma MK, Sharma R, Ronald PC, Fischer RL, Zilberman D. 2013. Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm. Proceedings of the National Academy of Sciences. 110(19), 7934–7939. mla: Rodrigues, Jessica A., et al. “Imprinted Expression of Genes and Small RNA Is Associated with Localized Hypomethylation of the Maternal Genome in Rice Endosperm.” Proceedings of the National Academy of Sciences, vol. 110, no. 19, National Academy of Sciences, 2013, pp. 7934–39, doi:10.1073/pnas.1306164110. short: J.A. Rodrigues, R. Ruan, T. Nishimura, M.K. Sharma, R. Sharma, P.C. Ronald, R.L. Fischer, D. Zilberman, Proceedings of the National Academy of Sciences 110 (2013) 7934–7939. date_created: 2021-06-07T07:31:02Z date_published: 2013-05-07T00:00:00Z date_updated: 2021-12-14T08:26:44Z day: '07' department: - _id: DaZi doi: 10.1073/pnas.1306164110 extern: '1' external_id: pmid: - '23613580' intvolume: ' 110' issue: '19' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1073/pnas.1306164110 month: '05' oa: 1 oa_version: Published Version page: 7934-7939 pmid: 1 publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' scopus_import: '1' status: public title: Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 110 year: '2013' ...