---
OA_type: closed access
_id: '3750'
abstract:
- lang: eng
  text: We applied a single-cell assay to characterize how transcription dynamics
    affects protein expression levels of a tetracycline-inducible gene expression
    system. Transcriptional activity of the tetracycline promoter in response to a
    steady level of inducer is steady in ΔacrAB efflux mutant but pulsating in wildtype
    Escherichia coli cells. We found that the expression level of the green fluorescent
    protein is several folds higher in ΔacrAB efflux mutant than in wildtype cells.
article_processing_charge: No
article_type: original
author:
- first_name: Thuc
  full_name: Le, Thuc
  last_name: Le
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Philippe
  full_name: Cluzel, Philippe
  last_name: Cluzel
citation:
  ama: Le T, Guet CC, Cluzel P. Protein expression enhancement in efflux-deleted mutant
    bacteria. <i>Protein Expression and Purification</i>. 2006;48(1):28-31.
  apa: Le, T., Guet, C. C., &#38; Cluzel, P. (2006). Protein expression enhancement
    in efflux-deleted mutant bacteria. <i>Protein Expression and Purification</i>.
    Elsevier.
  chicago: Le, Thuc, Calin C Guet, and Philippe Cluzel. “Protein Expression Enhancement
    in Efflux-Deleted Mutant Bacteria.” <i>Protein Expression and Purification</i>.
    Elsevier, 2006.
  ieee: T. Le, C. C. Guet, and P. Cluzel, “Protein expression enhancement in efflux-deleted
    mutant bacteria,” <i>Protein Expression and Purification</i>, vol. 48, no. 1.
    Elsevier, pp. 28–31, 2006.
  ista: Le T, Guet CC, Cluzel P. 2006. Protein expression enhancement in efflux-deleted
    mutant bacteria. Protein Expression and Purification. 48(1), 28–31.
  mla: Le, Thuc, et al. “Protein Expression Enhancement in Efflux-Deleted Mutant Bacteria.”
    <i>Protein Expression and Purification</i>, vol. 48, no. 1, Elsevier, 2006, pp.
    28–31.
  short: T. Le, C.C. Guet, P. Cluzel, Protein Expression and Purification 48 (2006)
    28–31.
date_created: 2018-12-11T12:04:58Z
date_published: 2006-07-01T00:00:00Z
date_updated: 2026-08-27T10:14:38Z
day: '01'
extern: '1'
external_id:
  pmid:
  - '16403646'
intvolume: '        48'
issue: '1'
language:
- iso: eng
month: '07'
oa_version: None
page: 28 - 31
pmid: 1
publication: Protein Expression and Purification
publication_identifier:
  eissn:
  - 1096-0279
  issn:
  - 1046-5928
publication_status: published
publisher: Elsevier
publist_id: '2478'
status: public
title: Protein expression enhancement in efflux-deleted mutant bacteria
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 48
year: '2006'
...