---
OA_place: publisher
OA_type: hybrid
_id: '15408'
abstract:
- lang: eng
  text: "Background: IgE-mediated degranulation of mast cells (MCs) provides rapid
    protection against environmental hazards, including animal venoms. A fraction
    of tissue-resident MCs intimately associates with blood vessels. These perivascular
    MCs were reported to extend projections into the vessel lumen and to be the first
    MCs to acquire intravenously injected IgE, suggesting that IgE loading of MCs
    depends on their vascular association.\r\nObjective: We sought to elucidate the
    molecular basis of the MC–blood vessel interaction and to determine its relevance
    for IgE-mediated immune responses.\r\nMethods: We selectively inactivated the
    Itgb1 gene, encoding the β1 chain of integrin adhesion molecules (ITGB1), in MCs
    by conditional gene targeting in mice. We analyzed skin MCs for blood vessel association,
    surface IgE density, and capability to bind circulating antibody specific for
    MC surface molecules, as well as in vivo responses to antigen administered via
    different routes.\r\nResults: Lack of ITGB1 expression severely compromised MC–blood
    vessel association. ITGB1-deficient MCs showed normal densities of surface IgE
    but reduced binding of intravenously injected antibodies. While their capacity
    to degranulate in response to IgE ligation in vivo was unimpaired, anaphylactic
    responses to antigen circulating in the vasculature were largely abolished.\r\nConclusions:
    ITGB1-mediated association of MCs with blood vessels is key for MC immune surveillance
    of blood vessel content, but is dispensable for slow steady-state loading of endogenous
    IgE onto tissue-resident MCs."
acknowledgement: "This work was funded by Deutsche Forschungsgemeinschaft, Germany,
  grants RO2133/ 9-1 and RO2133/ 9-2 in the setting of FOR2599 and TR156 project C11
  (Project-ID 246807620–TRR 156) to A. Roers and Springboard-to-Postdoc grant of the
  Dresden International Graduate School for Biomedicine and Bioengineering (DIGS-BB),
  Dresden, Germany, and Fond zur Förderung der Wissenschaftlichen Forschung (FWF),
  Austria, Hertha Firnberg grant (project number T-1219) to A. Polikarpova.\r\nWe
  thank Dr Michael Gerlach, Core Facility Cellular Imaging, Faculty of Medicine Carl
  Gustav Carus, TU Dresden, for expert support of in vivo imaging experiments; Grace
  Wurigamule for help with 2-photon imaging and flow cytometric analysis of mouse
  skin; and Christina Hiller, Livia Schulze, Madelaine Rickauer, and Christa Haase
  for providing expert technical assistance."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Kristina
  full_name: Link, Kristina
  last_name: Link
- first_name: Lina
  full_name: Muhandes, Lina
  last_name: Muhandes
- first_name: Anastasia
  full_name: Polikarpova, Anastasia
  last_name: Polikarpova
- first_name: Tim
  full_name: Lämmermann, Tim
  last_name: Lämmermann
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Reinhard
  full_name: Fässler, Reinhard
  last_name: Fässler
- first_name: Axel
  full_name: Roers, Axel
  last_name: Roers
citation:
  ama: Link K, Muhandes L, Polikarpova A, et al. Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. 2024;154(3):745-753.
    doi:<a href="https://doi.org/10.1016/j.jaci.2024.03.022">10.1016/j.jaci.2024.03.022</a>
  apa: Link, K., Muhandes, L., Polikarpova, A., Lämmermann, T., Sixt, M. K., Fässler,
    R., &#38; Roers, A. (2024). Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.jaci.2024.03.022">https://doi.org/10.1016/j.jaci.2024.03.022</a>
  chicago: Link, Kristina, Lina Muhandes, Anastasia Polikarpova, Tim Lämmermann, Michael
    K Sixt, Reinhard Fässler, and Axel Roers. “Integrin Β1–Mediated Mast Cell Immune-Surveillance
    of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>. Elsevier,
    2024. <a href="https://doi.org/10.1016/j.jaci.2024.03.022">https://doi.org/10.1016/j.jaci.2024.03.022</a>.
  ieee: K. Link <i>et al.</i>, “Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content,” <i>Journal of Allergy and Clinical Immunology</i>, vol.
    154, no. 3. Elsevier, pp. 745–753, 2024.
  ista: Link K, Muhandes L, Polikarpova A, Lämmermann T, Sixt MK, Fässler R, Roers
    A. 2024. Integrin β1–mediated mast cell immune-surveillance of blood vessel content.
    Journal of Allergy and Clinical Immunology. 154(3), 745–753.
  mla: Link, Kristina, et al. “Integrin Β1–Mediated Mast Cell Immune-Surveillance
    of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>, vol.
    154, no. 3, Elsevier, 2024, pp. 745–53, doi:<a href="https://doi.org/10.1016/j.jaci.2024.03.022">10.1016/j.jaci.2024.03.022</a>.
  short: K. Link, L. Muhandes, A. Polikarpova, T. Lämmermann, M.K. Sixt, R. Fässler,
    A. Roers, Journal of Allergy and Clinical Immunology 154 (2024) 745–753.
date_created: 2024-05-19T22:01:13Z
date_published: 2024-09-01T00:00:00Z
date_updated: 2025-09-08T07:28:25Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1016/j.jaci.2024.03.022
external_id:
  isi:
  - '001308886700001'
  pmid:
  - '38636606'
file:
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  creator: dernst
  date_created: 2025-01-13T10:55:28Z
  date_updated: 2025-01-13T10:55:28Z
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has_accepted_license: '1'
intvolume: '       154'
isi: 1
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: 745-753
pmid: 1
publication: Journal of Allergy and Clinical Immunology
publication_identifier:
  eissn:
  - 1097-6825
  issn:
  - 0091-6749
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Integrin β1–mediated mast cell immune-surveillance of blood vessel content
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volume: 154
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