[{"dataavailabilitystatement":"Biological materials (seeds, plasmids) are available upon request from ST (sttan@ustc.edu.cn). The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.","keyword":["auxin","SAC1","GRV2","PIN3","vacuole","gravitropism","Arabidopsis"],"abstract":[{"lang":"eng","text":"Gravitropism is a fundamental adaptive response in plants that enables directional growth to optimize resource acquisition. In this study, we employed forward genetic screening to identify Arabidopsis mutants with defective hypocotyl gravitropism and isolated the short and agravitropic hypocotyl in dark1 (sad1) mutant, which carries a point mutation (G110E) in the SAC1 gene encoding a phosphoinositide phosphatase. Deficiency of SAC1 disrupted gravity-induced polar localization of PIN3 in endodermal cells, impairing auxin redistribution and leading to hypocotyl gravitropism defects. Subcellular localization analysis revealed that SAC1 is partially localized to the PVC/tonoplast and participates in late endosomal trafficking. The sac1 mutation leads to abnormal vacuolar morphology, which is associated with defects in amyloplast sedimentation during the gravitropic response in Arabidopsis shoots. We further revealed that SAC1 interacts with GRV2, a key regulator of the late endocytic pathway, and that both proteins cooperatively regulate shoot gravitropism. In summary, this study identified SAC1 as a regulator of shoot gravitropism, revealing its important role in modulating vacuolar homeostasis, amyloplast sedimentation, PIN3 trafficking, and auxin distribution. These findings provide insights into the molecular mechanisms linking membrane transport to environmental adaptation in plants."}],"title":"Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis","publisher":"Wiley","scopus_import":"1","year":"2026","pmid":1,"external_id":{"pmid":["42438075"]},"OA_type":"closed access","article_processing_charge":"No","_id":"22366","quality_controlled":"1","doi":"10.1111/tpj.71042","language":[{"iso":"eng"}],"volume":127,"issue":"1","citation":{"short":"L. Sun, W. Jia, Y. Mao, X. Li, M. Kong, J. She, J. Friml, S. Tan, The Plant Journal 127 (2026).","chicago":"Sun, Lianghanxiao, Wenxin Jia, Yanbo Mao, Xin Li, Mengjuan Kong, Ji She, Jiří Friml, and Shutang Tan. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” <i>The Plant Journal</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/tpj.71042\">https://doi.org/10.1111/tpj.71042</a>.","ista":"Sun L, Jia W, Mao Y, Li X, Kong M, She J, Friml J, Tan S. 2026. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 127(1), e71042.","mla":"Sun, Lianghanxiao, et al. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” <i>The Plant Journal</i>, vol. 127, no. 1, e71042, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/tpj.71042\">10.1111/tpj.71042</a>.","ieee":"L. Sun <i>et al.</i>, “Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis,” <i>The Plant Journal</i>, vol. 127, no. 1. Wiley, 2026.","ama":"Sun L, Jia W, Mao Y, et al. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. <i>The Plant Journal</i>. 2026;127(1). doi:<a href=\"https://doi.org/10.1111/tpj.71042\">10.1111/tpj.71042</a>","apa":"Sun, L., Jia, W., Mao, Y., Li, X., Kong, M., She, J., … Tan, S. (2026). Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.71042\">https://doi.org/10.1111/tpj.71042</a>"},"month":"07","date_created":"2026-07-19T22:01:47Z","status":"public","das_tickbox":"1","oa_version":"None","article_number":"e71042","supplementarymaterial":"yes","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","researchdata_availability":"upon request","publication_identifier":{"eissn":["1365-313X"],"issn":["0960-7412"]},"author":[{"first_name":"Lianghanxiao","full_name":"Sun, Lianghanxiao","last_name":"Sun"},{"full_name":"Jia, Wenxin","first_name":"Wenxin","last_name":"Jia"},{"first_name":"Yanbo","full_name":"Mao, Yanbo","last_name":"Mao"},{"first_name":"Xin","full_name":"Li, Xin","last_name":"Li"},{"first_name":"Mengjuan","full_name":"Kong, Mengjuan","last_name":"Kong"},{"first_name":"Ji","full_name":"She, Ji","last_name":"She"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","orcid":"0000-0002-0471-8285","full_name":"Tan, Shutang","first_name":"Shutang"}],"date_updated":"2026-07-20T13:53:44Z","acknowledgement":"We acknowledge Prof. Dolf Weijers (Wageningen University), Prof.Karin Schumacher (Heidelberg University), Prof. Yohann Boutt ´e(Universit ´e de Bordeaux), and Prof. Jinbo Shen (Zhejiang A&FUniversity) for providing published plasmids and Arabidopsislines. We thank Dr. Gergely Moln ´ar (ISTA) for help with NGS dataanalysis, and Prof. Jianru Zuo (IGDB, CAS), Prof. Chengbin Xiang(USTC), and Prof. Zhong Zhao (USTC) for critical comments onthe manuscript. We thank the staff members of the Mass Spec-trometry System at the National Facility for Protein Science inShanghai (NFPS), Zhangjiang Lab, China for providing technicalsupport and assistance in data collection and analysis. This workwas supported by grants from the National Natural Science Foun-dation of China (32570366, and 32321001 to ST), the Natural Sci-ence Foundation of Anhui Province (2508085QC070 to MK), theFundamental Research Funds for the Central Universities(WK9100250095 to MK, and WK9100000021 to ST), the ForestryBureau of Anhui Province (AHLYJBGS-2024-01 to ST), the Centerfor Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine, University of Science andTechnology of China (QYPY20220012 to ST), the USTC ResearchFunds of the Double First-Class Initiative (YD9100002016 to ST),and start-up funding from the University of Science and Technol-ogy of China and the Chinese Academy of Sciences(GG9100007007, KY9100000026, KY9100000051, XKTS-202591014,XKTS-2026910122, and KJ2070000079 to ST).","publication":"The Plant Journal","type":"journal_article","department":[{"_id":"JiFr"}],"day":"01","date_published":"2026-07-01T00:00:00Z","intvolume":"       127","publication_status":"published"},{"status":"public","date_created":"2025-08-17T22:01:36Z","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"month":"08","citation":{"short":"D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).","ista":"Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396.","chicago":"Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>.","ama":"Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. 2025;123(3). doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>","mla":"Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>, vol. 123, no. 3, e70396, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>.","ieee":"D. Babic <i>et al.</i>, “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” <i>Plant Journal</i>, vol. 123, no. 3. Wiley, 2025.","apa":"Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>"},"issue":"3","volume":123,"language":[{"iso":"eng"}],"OA_place":"publisher","doi":"10.1111/tpj.70396","ddc":["580"],"quality_controlled":"1","_id":"20187","article_processing_charge":"Yes (via OA deal)","oa":1,"external_id":{"isi":["001547884300001"],"pmid":["40782342"]},"OA_type":"hybrid","pmid":1,"year":"2025","has_accepted_license":"1","scopus_import":"1","publisher":"Wiley","title":"Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development."}],"publication_status":"published","file":[{"success":1,"date_created":"2025-09-01T14:09:31Z","date_updated":"2025-09-01T14:09:31Z","relation":"main_file","file_name":"2025_PlantJournal_Babic.pdf","file_size":5791111,"access_level":"open_access","content_type":"application/pdf","file_id":"20264","creator":"dernst","checksum":"1cdc3341d2d23101abca72521f1f23cb"}],"intvolume":"       123","date_published":"2025-08-01T00:00:00Z","file_date_updated":"2025-09-01T14:09:31Z","day":"01","PlanS_conform":"1","department":[{"_id":"EvBe"},{"_id":"JiFr"},{"_id":"GradSch"}],"isi":1,"type":"journal_article","related_material":{"record":[{"status":"public","id":"20362","relation":"dissertation_contains"}]},"publication":"Plant Journal","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"corr_author":"1","acknowledgement":"We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","date_updated":"2026-04-07T11:52:02Z","author":[{"full_name":"Babic, David","first_name":"David","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","last_name":"Babic"},{"first_name":"Rashed","full_name":"Abualia, Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9357-9415","last_name":"Abualia"},{"first_name":"Lukas","full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler"},{"id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi","orcid":"0000-0001-5187-8401","full_name":"Qi, Linlin","first_name":"Linlin"},{"last_name":"Tellier","full_name":"Tellier, Frédérique","first_name":"Frédérique"},{"first_name":"Adrijana","full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan"},{"last_name":"Rakusova","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","first_name":"Hana","full_name":"Rakusova, Hana"},{"first_name":"Petr","full_name":"Valošek, Petr","last_name":"Valošek","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Han, Huibin","first_name":"Huibin","last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková"},{"full_name":"Faure, Jean Denis","first_name":"Jean Denis","last_name":"Faure"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří"}],"publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"e70396","article_type":"original","oa_version":"Published Version"},{"scopus_import":"1","year":"2023","pmid":1,"abstract":[{"text":"Salicylic acid (SA) plays important roles in different aspects of plant development, including root growth, where auxin is also a major player by means of its asymmetric distribution. However, the mechanism underlying the effect of SA on the development of rice roots remains poorly understood. Here, we show that SA inhibits rice root growth by interfering with auxin transport associated with the OsPIN3t- and clathrin-mediated gene regulatory network (GRN). SA inhibits root growth as well as Brefeldin A-sensitive trafficking through a non-canonical SA signaling mechanism. Transcriptome analysis of rice seedlings treated with SA revealed that the OsPIN3t auxin transporter is at the center of a GRN involving the coat protein clathrin. The root growth and endocytic trafficking in both the pin3t and clathrin heavy chain mutants were SA insensitivity. SA inhibitory effect on the endocytosis of OsPIN3t was dependent on clathrin; however, the root growth and endocytic trafficking mediated by tyrphostin A23 (TyrA23) were independent of the pin3t mutant under SA treatment. These data reveal that SA affects rice root growth through the convergence of transcriptional and non-SA signaling mechanisms involving OsPIN3t-mediated auxin transport and clathrin-mediated trafficking as key components.","lang":"eng"}],"title":"Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth","publisher":"Wiley","OA_place":"publisher","doi":"10.1111/tpj.16218","language":[{"iso":"eng"}],"volume":115,"citation":{"short":"L. Jiang, B. Yao, X. Zhang, L. Wu, Q. Fu, Y. Zhao, Y. Cao, R. Zhu, X. Lu, W. Huang, J. Zhao, K. Li, S. Zhao, L. Han, X. Zhou, C. Luo, H. Zhu, J. Yang, H. Huang, Z. Zhu, X. He, J. Friml, Z. Zhang, C. Liu, Y. Du, Plant Journal 115 (2023) 155–174.","ista":"Jiang L, Yao B, Zhang X, Wu L, Fu Q, Zhao Y, Cao Y, Zhu R, Lu X, Huang W, Zhao J, Li K, Zhao S, Han L, Zhou X, Luo C, Zhu H, Yang J, Huang H, Zhu Z, He X, Friml J, Zhang Z, Liu C, Du Y. 2023. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. 115(1), 155–174.","chicago":"Jiang, Lihui, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao, Yuxin Cao, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>.","mla":"Jiang, Lihui, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>, vol. 115, no. 1, Wiley, 2023, pp. 155–74, doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>.","ieee":"L. Jiang <i>et al.</i>, “Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth,” <i>Plant Journal</i>, vol. 115, no. 1. Wiley, pp. 155–174, 2023.","ama":"Jiang L, Yao B, Zhang X, et al. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. 2023;115(1):155-174. doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>","apa":"Jiang, L., Yao, B., Zhang, X., Wu, L., Fu, Q., Zhao, Y., … Du, Y. (2023). Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>"},"issue":"1","month":"07","date_created":"2023-04-30T22:01:06Z","status":"public","OA_type":"free access","external_id":{"pmid":["37025008 "],"isi":["000971861400001"]},"oa":1,"quality_controlled":"1","_id":"12878","article_processing_charge":"No","ddc":["580"],"date_updated":"2026-06-18T17:29:30Z","acknowledgement":"The authors thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese Academy of Sciences) for support with phytohormone measurement. Thanks also go to Professor Pieter. B. F. Ouwerkerk (Leiden University) and Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision of the rice lines NB-7B-70 and NB-7B-76 and wild-type NB-61-WT, Professor Zuhua He (Chinese Academy of Sciences) for provision of the rice OsNPR1-RNAi mutant, and Professor Yinong Yang (The Pennsylvania State University) for provision of the rice line NahG. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 32260085, 31460453, 31660501, 31860064, 31970609, 31801792 and 31960554), the Key Projects of the Applied Basic Research Plan of Yunnan Province (202301AS070082), the Major Special Program for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005), the Start-up fund from Xishuangbanna Tropical Botanical Garden, and ‘Top Talents Program in Science and Technology’ from Yunnan Province, the SRF for ROCS, SEM (Grant No. [2013] 1792), and the Major Science and Technology Project in Yunnan Province (202102AE090042 and 202202AE090036); and the young and middle-aged academic and technical leaders reserve talent program in Yunnan Province (202205AC160076).","publication":"Plant Journal","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/tpj.16218"}],"oa_version":"Published Version","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"author":[{"full_name":"Jiang, Lihui","first_name":"Lihui","last_name":"Jiang"},{"last_name":"Yao","full_name":"Yao, Baolin","first_name":"Baolin"},{"full_name":"Zhang, Xiaoyan","first_name":"Xiaoyan","last_name":"Zhang"},{"full_name":"Wu, Lixia","first_name":"Lixia","last_name":"Wu"},{"first_name":"Qijing","full_name":"Fu, Qijing","last_name":"Fu"},{"last_name":"Zhao","full_name":"Zhao, Yiting","first_name":"Yiting"},{"first_name":"Yuxin","full_name":"Cao, Yuxin","last_name":"Cao"},{"last_name":"Zhu","first_name":"Ruomeng","full_name":"Zhu, Ruomeng"},{"full_name":"Lu, Xinqi","first_name":"Xinqi","last_name":"Lu"},{"full_name":"Huang, Wuying","first_name":"Wuying","last_name":"Huang"},{"last_name":"Zhao","full_name":"Zhao, Jianping","first_name":"Jianping"},{"last_name":"Li","first_name":"Kuixiu","full_name":"Li, Kuixiu"},{"last_name":"Zhao","first_name":"Shuanglu","full_name":"Zhao, Shuanglu"},{"last_name":"Han","full_name":"Han, Li","first_name":"Li"},{"full_name":"Zhou, Xuan","first_name":"Xuan","last_name":"Zhou"},{"last_name":"Luo","first_name":"Chongyu","full_name":"Luo, Chongyu"},{"full_name":"Zhu, Haiyan","first_name":"Haiyan","last_name":"Zhu"},{"last_name":"Yang","first_name":"Jing","full_name":"Yang, Jing"},{"last_name":"Huang","first_name":"Huichuan","full_name":"Huang, Huichuan"},{"full_name":"Zhu, Zhengge","first_name":"Zhengge","last_name":"Zhu"},{"last_name":"He","first_name":"Xiahong","full_name":"He, Xiahong"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"first_name":"Zhongkai","full_name":"Zhang, Zhongkai","last_name":"Zhang"},{"last_name":"Liu","first_name":"Changning","full_name":"Liu, Changning"},{"full_name":"Du, Yunlong","first_name":"Yunlong","last_name":"Du"}],"intvolume":"       115","publication_status":"published","isi":1,"page":"155-174","type":"journal_article","department":[{"_id":"JiFr"}],"day":"01","date_published":"2023-07-01T00:00:00Z"},{"_id":"2993","quality_controlled":"1","article_processing_charge":"No","OA_type":"closed access","external_id":{"pmid":["12662314"]},"issue":"1","citation":{"ama":"Friml J, Benková E, Mayer U, Palme K, Muster G. Automated whole mount localisation techniques for plant seedlings. <i>Plant Journal</i>. 2003;34(1):115-124. doi:<a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">10.1046/j.1365-313X.2003.01705.x</a>","ieee":"J. Friml, E. Benková, U. Mayer, K. Palme, and G. Muster, “Automated whole mount localisation techniques for plant seedlings,” <i>Plant Journal</i>, vol. 34, no. 1. Wiley, pp. 115–124, 2003.","mla":"Friml, Jiří, et al. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” <i>Plant Journal</i>, vol. 34, no. 1, Wiley, 2003, pp. 115–24, doi:<a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">10.1046/j.1365-313X.2003.01705.x</a>.","apa":"Friml, J., Benková, E., Mayer, U., Palme, K., &#38; Muster, G. (2003). Automated whole mount localisation techniques for plant seedlings. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>","short":"J. Friml, E. Benková, U. Mayer, K. Palme, G. Muster, Plant Journal 34 (2003) 115–124.","ista":"Friml J, Benková E, Mayer U, Palme K, Muster G. 2003. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 34(1), 115–124.","chicago":"Friml, Jiří, Eva Benková, Ulrike Mayer, Klaus Palme, and Gerhard Muster. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” <i>Plant Journal</i>. Wiley, 2003. <a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>."},"month":"04","date_created":"2018-12-11T12:00:44Z","status":"public","doi":"10.1046/j.1365-313X.2003.01705.x","language":[{"iso":"eng"}],"volume":34,"title":"Automated whole mount localisation techniques for plant seedlings","publisher":"Wiley","abstract":[{"text":"Plant biology is currently experiencing a growing demand for easy and reliable mRNA and protein localisation techniques. Here, we present novel whole mount in situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs and proteins in Arabidopsis seedlings. We demonstrate that these methods can be used in different organs of Arabidopsis seedlings as well as in other plant species. In order to achieve better reproducibility and higher throughput, we modified these protocols for automation to be performed by a liquid handling robot. In addition, we show that other procedures such as reporter enzyme assays and tissue clearing can be similarly automated. We present examples of application of our protocols including mRNA localisation and proteins and epitope tag (co)localisations which demonstrate that these methods provide reliable and versatile tools for expression, localisation and anatomical studies in plants.","lang":"eng"}],"pmid":1,"scopus_import":"1","year":"2003","extern":"1","date_published":"2003-04-01T00:00:00Z","page":"115 - 124","type":"journal_article","day":"01","intvolume":"        34","publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publist_id":"3709","author":[{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková"},{"last_name":"Mayer","first_name":"Ulrike","full_name":"Mayer, Ulrike"},{"last_name":"Palme","first_name":"Klaus","full_name":"Palme, Klaus"},{"last_name":"Muster","full_name":"Muster, Gerhard","first_name":"Gerhard"}],"publication_identifier":{"eissn":["1365-313X"],"issn":["0960-7412"]},"oa_version":"None","article_type":"original","publication":"Plant Journal","date_updated":"2026-05-22T09:01:28Z"}]