--- OA_type: closed access _id: '22366' abstract: - lang: eng text: Gravitropism is a fundamental adaptive response in plants that enables directional growth to optimize resource acquisition. In this study, we employed forward genetic screening to identify Arabidopsis mutants with defective hypocotyl gravitropism and isolated the short and agravitropic hypocotyl in dark1 (sad1) mutant, which carries a point mutation (G110E) in the SAC1 gene encoding a phosphoinositide phosphatase. Deficiency of SAC1 disrupted gravity-induced polar localization of PIN3 in endodermal cells, impairing auxin redistribution and leading to hypocotyl gravitropism defects. Subcellular localization analysis revealed that SAC1 is partially localized to the PVC/tonoplast and participates in late endosomal trafficking. The sac1 mutation leads to abnormal vacuolar morphology, which is associated with defects in amyloplast sedimentation during the gravitropic response in Arabidopsis shoots. We further revealed that SAC1 interacts with GRV2, a key regulator of the late endocytic pathway, and that both proteins cooperatively regulate shoot gravitropism. In summary, this study identified SAC1 as a regulator of shoot gravitropism, revealing its important role in modulating vacuolar homeostasis, amyloplast sedimentation, PIN3 trafficking, and auxin distribution. These findings provide insights into the molecular mechanisms linking membrane transport to environmental adaptation in plants. acknowledgement: We acknowledge Prof. Dolf Weijers (Wageningen University), Prof.Karin Schumacher (Heidelberg University), Prof. Yohann Boutt ´e(Universit ´e de Bordeaux), and Prof. Jinbo Shen (Zhejiang A&FUniversity) for providing published plasmids and Arabidopsislines. We thank Dr. Gergely Moln ´ar (ISTA) for help with NGS dataanalysis, and Prof. Jianru Zuo (IGDB, CAS), Prof. Chengbin Xiang(USTC), and Prof. Zhong Zhao (USTC) for critical comments onthe manuscript. We thank the staff members of the Mass Spec-trometry System at the National Facility for Protein Science inShanghai (NFPS), Zhangjiang Lab, China for providing technicalsupport and assistance in data collection and analysis. This workwas supported by grants from the National Natural Science Foun-dation of China (32570366, and 32321001 to ST), the Natural Sci-ence Foundation of Anhui Province (2508085QC070 to MK), theFundamental Research Funds for the Central Universities(WK9100250095 to MK, and WK9100000021 to ST), the ForestryBureau of Anhui Province (AHLYJBGS-2024-01 to ST), the Centerfor Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine, University of Science andTechnology of China (QYPY20220012 to ST), the USTC ResearchFunds of the Double First-Class Initiative (YD9100002016 to ST),and start-up funding from the University of Science and Technol-ogy of China and the Chinese Academy of Sciences(GG9100007007, KY9100000026, KY9100000051, XKTS-202591014,XKTS-2026910122, and KJ2070000079 to ST). article_number: e71042 article_processing_charge: No article_type: original author: - first_name: Lianghanxiao full_name: Sun, Lianghanxiao last_name: Sun - first_name: Wenxin full_name: Jia, Wenxin last_name: Jia - first_name: Yanbo full_name: Mao, Yanbo last_name: Mao - first_name: Xin full_name: Li, Xin last_name: Li - first_name: Mengjuan full_name: Kong, Mengjuan last_name: Kong - first_name: Ji full_name: She, Ji last_name: She - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Shutang full_name: Tan, Shutang id: 2DE75584-F248-11E8-B48F-1D18A9856A87 last_name: Tan orcid: 0000-0002-0471-8285 citation: ama: Sun L, Jia W, Mao Y, et al. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 2026;127(1). doi:10.1111/tpj.71042 apa: Sun, L., Jia, W., Mao, Y., Li, X., Kong, M., She, J., … Tan, S. (2026). Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. Wiley. https://doi.org/10.1111/tpj.71042 chicago: Sun, Lianghanxiao, Wenxin Jia, Yanbo Mao, Xin Li, Mengjuan Kong, Ji She, Jiří Friml, and Shutang Tan. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal. Wiley, 2026. https://doi.org/10.1111/tpj.71042. ieee: L. Sun et al., “Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis,” The Plant Journal, vol. 127, no. 1. Wiley, 2026. ista: Sun L, Jia W, Mao Y, Li X, Kong M, She J, Friml J, Tan S. 2026. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 127(1), e71042. mla: Sun, Lianghanxiao, et al. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” The Plant Journal, vol. 127, no. 1, e71042, Wiley, 2026, doi:10.1111/tpj.71042. short: L. Sun, W. Jia, Y. Mao, X. Li, M. Kong, J. She, J. Friml, S. Tan, The Plant Journal 127 (2026). das_tickbox: '1' dataavailabilitystatement: Biological materials (seeds, plasmids) are available upon request from ST (sttan@ustc.edu.cn). The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. date_created: 2026-07-19T22:01:47Z date_published: 2026-07-01T00:00:00Z date_updated: 2026-07-20T13:53:44Z day: '01' department: - _id: JiFr doi: 10.1111/tpj.71042 external_id: pmid: - '42438075' intvolume: ' 127' issue: '1' keyword: - auxin - SAC1 - GRV2 - PIN3 - vacuole - gravitropism - Arabidopsis language: - iso: eng month: '07' oa_version: None pmid: 1 publication: The Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 127 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20187' abstract: - lang: eng text: Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development. acknowledged_ssus: - _id: Bio - _id: EM-Fac - _id: LifeSc acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007)." article_number: e70396 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: David full_name: Babic, David id: db566d23-f6e0-11ea-865d-e6f270e968e7 last_name: Babic - first_name: Rashed full_name: Abualia, Rashed id: 4827E134-F248-11E8-B48F-1D18A9856A87 last_name: Abualia orcid: 0000-0002-9357-9415 - first_name: Lukas full_name: Fiedler, Lukas id: 7c417475-8972-11ed-ae7b-8b674ca26986 last_name: Fiedler - first_name: Linlin full_name: Qi, Linlin id: 44B04502-A9ED-11E9-B6FC-583AE6697425 last_name: Qi orcid: 0000-0001-5187-8401 - first_name: Frédérique full_name: Tellier, Frédérique last_name: Tellier - first_name: Adrijana full_name: Smoljan, Adrijana id: cced8a85-223e-11ed-af04-b0596c55053b last_name: Smoljan - first_name: Hana full_name: Rakusova, Hana id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA last_name: Rakusova - first_name: Petr full_name: Valošek, Petr id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87 last_name: Valošek - first_name: Huibin full_name: Han, Huibin id: 31435098-F248-11E8-B48F-1D18A9856A87 last_name: Han - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Jean Denis full_name: Faure, Jean Denis last_name: Faure - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396 apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. Wiley. https://doi.org/10.1111/tpj.70396 chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396. ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant Journal, vol. 123, no. 3. Wiley, 2025. ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396. mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396. short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025). corr_author: '1' date_created: 2025-08-17T22:01:36Z date_published: 2025-08-01T00:00:00Z date_updated: 2026-04-07T11:52:02Z day: '01' ddc: - '580' department: - _id: EvBe - _id: JiFr - _id: GradSch doi: 10.1111/tpj.70396 external_id: isi: - '001547884300001' pmid: - '40782342' file: - access_level: open_access checksum: 1cdc3341d2d23101abca72521f1f23cb content_type: application/pdf creator: dernst date_created: 2025-09-01T14:09:31Z date_updated: 2025-09-01T14:09:31Z file_id: '20264' file_name: 2025_PlantJournal_Babic.pdf file_size: 5791111 relation: main_file success: 1 file_date_updated: 2025-09-01T14:09:31Z has_accepted_license: '1' intvolume: ' 123' isi: 1 issue: '3' language: - iso: eng month: '08' oa: 1 oa_version: Published Version pmid: 1 project: - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis publication: Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley quality_controlled: '1' related_material: record: - id: '20362' relation: dissertation_contains status: public scopus_import: '1' status: public title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 123 year: '2025' ... --- OA_place: publisher OA_type: free access _id: '12878' abstract: - lang: eng text: Salicylic acid (SA) plays important roles in different aspects of plant development, including root growth, where auxin is also a major player by means of its asymmetric distribution. However, the mechanism underlying the effect of SA on the development of rice roots remains poorly understood. Here, we show that SA inhibits rice root growth by interfering with auxin transport associated with the OsPIN3t- and clathrin-mediated gene regulatory network (GRN). SA inhibits root growth as well as Brefeldin A-sensitive trafficking through a non-canonical SA signaling mechanism. Transcriptome analysis of rice seedlings treated with SA revealed that the OsPIN3t auxin transporter is at the center of a GRN involving the coat protein clathrin. The root growth and endocytic trafficking in both the pin3t and clathrin heavy chain mutants were SA insensitivity. SA inhibitory effect on the endocytosis of OsPIN3t was dependent on clathrin; however, the root growth and endocytic trafficking mediated by tyrphostin A23 (TyrA23) were independent of the pin3t mutant under SA treatment. These data reveal that SA affects rice root growth through the convergence of transcriptional and non-SA signaling mechanisms involving OsPIN3t-mediated auxin transport and clathrin-mediated trafficking as key components. acknowledgement: The authors thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese Academy of Sciences) for support with phytohormone measurement. Thanks also go to Professor Pieter. B. F. Ouwerkerk (Leiden University) and Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision of the rice lines NB-7B-70 and NB-7B-76 and wild-type NB-61-WT, Professor Zuhua He (Chinese Academy of Sciences) for provision of the rice OsNPR1-RNAi mutant, and Professor Yinong Yang (The Pennsylvania State University) for provision of the rice line NahG. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 32260085, 31460453, 31660501, 31860064, 31970609, 31801792 and 31960554), the Key Projects of the Applied Basic Research Plan of Yunnan Province (202301AS070082), the Major Special Program for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005), the Start-up fund from Xishuangbanna Tropical Botanical Garden, and ‘Top Talents Program in Science and Technology’ from Yunnan Province, the SRF for ROCS, SEM (Grant No. [2013] 1792), and the Major Science and Technology Project in Yunnan Province (202102AE090042 and 202202AE090036); and the young and middle-aged academic and technical leaders reserve talent program in Yunnan Province (202205AC160076). article_processing_charge: No article_type: original author: - first_name: Lihui full_name: Jiang, Lihui last_name: Jiang - first_name: Baolin full_name: Yao, Baolin last_name: Yao - first_name: Xiaoyan full_name: Zhang, Xiaoyan last_name: Zhang - first_name: Lixia full_name: Wu, Lixia last_name: Wu - first_name: Qijing full_name: Fu, Qijing last_name: Fu - first_name: Yiting full_name: Zhao, Yiting last_name: Zhao - first_name: Yuxin full_name: Cao, Yuxin last_name: Cao - first_name: Ruomeng full_name: Zhu, Ruomeng last_name: Zhu - first_name: Xinqi full_name: Lu, Xinqi last_name: Lu - first_name: Wuying full_name: Huang, Wuying last_name: Huang - first_name: Jianping full_name: Zhao, Jianping last_name: Zhao - first_name: Kuixiu full_name: Li, Kuixiu last_name: Li - first_name: Shuanglu full_name: Zhao, Shuanglu last_name: Zhao - first_name: Li full_name: Han, Li last_name: Han - first_name: Xuan full_name: Zhou, Xuan last_name: Zhou - first_name: Chongyu full_name: Luo, Chongyu last_name: Luo - first_name: Haiyan full_name: Zhu, Haiyan last_name: Zhu - first_name: Jing full_name: Yang, Jing last_name: Yang - first_name: Huichuan full_name: Huang, Huichuan last_name: Huang - first_name: Zhengge full_name: Zhu, Zhengge last_name: Zhu - first_name: Xiahong full_name: He, Xiahong last_name: He - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Zhongkai full_name: Zhang, Zhongkai last_name: Zhang - first_name: Changning full_name: Liu, Changning last_name: Liu - first_name: Yunlong full_name: Du, Yunlong last_name: Du citation: ama: Jiang L, Yao B, Zhang X, et al. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. 2023;115(1):155-174. doi:10.1111/tpj.16218 apa: Jiang, L., Yao, B., Zhang, X., Wu, L., Fu, Q., Zhao, Y., … Du, Y. (2023). Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. Wiley. https://doi.org/10.1111/tpj.16218 chicago: Jiang, Lihui, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao, Yuxin Cao, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” Plant Journal. Wiley, 2023. https://doi.org/10.1111/tpj.16218. ieee: L. Jiang et al., “Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth,” Plant Journal, vol. 115, no. 1. Wiley, pp. 155–174, 2023. ista: Jiang L, Yao B, Zhang X, Wu L, Fu Q, Zhao Y, Cao Y, Zhu R, Lu X, Huang W, Zhao J, Li K, Zhao S, Han L, Zhou X, Luo C, Zhu H, Yang J, Huang H, Zhu Z, He X, Friml J, Zhang Z, Liu C, Du Y. 2023. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. 115(1), 155–174. mla: Jiang, Lihui, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” Plant Journal, vol. 115, no. 1, Wiley, 2023, pp. 155–74, doi:10.1111/tpj.16218. short: L. Jiang, B. Yao, X. Zhang, L. Wu, Q. Fu, Y. Zhao, Y. Cao, R. Zhu, X. Lu, W. Huang, J. Zhao, K. Li, S. Zhao, L. Han, X. Zhou, C. Luo, H. Zhu, J. Yang, H. Huang, Z. Zhu, X. He, J. Friml, Z. Zhang, C. Liu, Y. Du, Plant Journal 115 (2023) 155–174. date_created: 2023-04-30T22:01:06Z date_published: 2023-07-01T00:00:00Z date_updated: 2026-06-18T17:29:30Z day: '01' ddc: - '580' department: - _id: JiFr doi: 10.1111/tpj.16218 external_id: isi: - '000971861400001' pmid: - '37025008 ' intvolume: ' 115' isi: 1 issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1111/tpj.16218 month: '07' oa: 1 oa_version: Published Version page: 155-174 pmid: 1 publication: Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley quality_controlled: '1' scopus_import: '1' status: public title: Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 115 year: '2023' ... --- OA_type: closed access _id: '2993' abstract: - lang: eng text: Plant biology is currently experiencing a growing demand for easy and reliable mRNA and protein localisation techniques. Here, we present novel whole mount in situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs and proteins in Arabidopsis seedlings. We demonstrate that these methods can be used in different organs of Arabidopsis seedlings as well as in other plant species. In order to achieve better reproducibility and higher throughput, we modified these protocols for automation to be performed by a liquid handling robot. In addition, we show that other procedures such as reporter enzyme assays and tissue clearing can be similarly automated. We present examples of application of our protocols including mRNA localisation and proteins and epitope tag (co)localisations which demonstrate that these methods provide reliable and versatile tools for expression, localisation and anatomical studies in plants. article_processing_charge: No article_type: original author: - first_name: Jirí full_name: Friml, Jirí id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Ulrike full_name: Mayer, Ulrike last_name: Mayer - first_name: Klaus full_name: Palme, Klaus last_name: Palme - first_name: Gerhard full_name: Muster, Gerhard last_name: Muster citation: ama: Friml J, Benková E, Mayer U, Palme K, Muster G. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 2003;34(1):115-124. doi:10.1046/j.1365-313X.2003.01705.x apa: Friml, J., Benková, E., Mayer, U., Palme, K., & Muster, G. (2003). Automated whole mount localisation techniques for plant seedlings. Plant Journal. Wiley. https://doi.org/10.1046/j.1365-313X.2003.01705.x chicago: Friml, Jiří, Eva Benková, Ulrike Mayer, Klaus Palme, and Gerhard Muster. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” Plant Journal. Wiley, 2003. https://doi.org/10.1046/j.1365-313X.2003.01705.x. ieee: J. Friml, E. Benková, U. Mayer, K. Palme, and G. Muster, “Automated whole mount localisation techniques for plant seedlings,” Plant Journal, vol. 34, no. 1. Wiley, pp. 115–124, 2003. ista: Friml J, Benková E, Mayer U, Palme K, Muster G. 2003. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 34(1), 115–124. mla: Friml, Jiří, et al. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” Plant Journal, vol. 34, no. 1, Wiley, 2003, pp. 115–24, doi:10.1046/j.1365-313X.2003.01705.x. short: J. Friml, E. Benková, U. Mayer, K. Palme, G. Muster, Plant Journal 34 (2003) 115–124. date_created: 2018-12-11T12:00:44Z date_published: 2003-04-01T00:00:00Z date_updated: 2026-05-22T09:01:28Z day: '01' doi: 10.1046/j.1365-313X.2003.01705.x extern: '1' external_id: pmid: - '12662314' intvolume: ' 34' issue: '1' language: - iso: eng month: '04' oa_version: None page: 115 - 124 pmid: 1 publication: Plant Journal publication_identifier: eissn: - 1365-313X issn: - 0960-7412 publication_status: published publisher: Wiley publist_id: '3709' quality_controlled: '1' scopus_import: '1' status: public title: Automated whole mount localisation techniques for plant seedlings type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 34 year: '2003' ...