---
_id: '13388'
abstract:
- lang: eng
text: The Inside Cover picture illustrates the fluorescent properties of a gold
nanocluster functionalized with several copies of a red-emitting merocyanine (image
by Ella Marushchenko). The red fluorescence can be turned on and off reversibly
by using an external stimulus.
article_processing_charge: No
author:
- first_name: T.
full_name: Udayabhaskararao, T.
last_name: Udayabhaskararao
- first_name: Pintu K.
full_name: Kundu, Pintu K.
last_name: Kundu
- first_name: Johannes
full_name: Ahrens, Johannes
last_name: Ahrens
- first_name: Rafal
full_name: Klajn, Rafal
id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b
last_name: Klajn
citation:
ama: 'Udayabhaskararao T, Kundu PK, Ahrens J, Klajn R. Inside Cover: Reversible
Photoisomerization of Spiropyran on the Surfaces of Au25 Nanoclusters (ChemPhysChem
12/2016). Vol 17. Wiley; 2016:1711-1711. doi:10.1002/cphc.201600480'
apa: 'Udayabhaskararao, T., Kundu, P. K., Ahrens, J., & Klajn, R. (2016). Inside
cover: Reversible photoisomerization of spiropyran on the surfaces of Au25 nanoclusters
(ChemPhysChem 12/2016). ChemPhysChem (Vol. 17, pp. 1711–1711). Wiley.
https://doi.org/10.1002/cphc.201600480'
chicago: 'Udayabhaskararao, T., Pintu K. Kundu, Johannes Ahrens, and Rafal Klajn.
Inside Cover: Reversible Photoisomerization of Spiropyran on the Surfaces of
Au25 Nanoclusters (ChemPhysChem 12/2016). ChemPhysChem. Vol. 17. Wiley,
2016. https://doi.org/10.1002/cphc.201600480.'
ieee: 'T. Udayabhaskararao, P. K. Kundu, J. Ahrens, and R. Klajn, Inside cover:
Reversible photoisomerization of spiropyran on the surfaces of Au25 nanoclusters
(ChemPhysChem 12/2016), vol. 17, no. 12. Wiley, 2016, pp. 1711–1711.'
ista: 'Udayabhaskararao T, Kundu PK, Ahrens J, Klajn R. 2016. Inside cover: Reversible
photoisomerization of spiropyran on the surfaces of Au25 nanoclusters (ChemPhysChem
12/2016), Wiley,p.'
mla: 'Udayabhaskararao, T., et al. “Inside Cover: Reversible Photoisomerization
of Spiropyran on the Surfaces of Au25 Nanoclusters (ChemPhysChem 12/2016).” ChemPhysChem,
vol. 17, no. 12, Wiley, 2016, pp. 1711–1711, doi:10.1002/cphc.201600480.'
short: 'T. Udayabhaskararao, P.K. Kundu, J. Ahrens, R. Klajn, Inside Cover: Reversible
Photoisomerization of Spiropyran on the Surfaces of Au25 Nanoclusters (ChemPhysChem
12/2016), Wiley, 2016.'
date_created: 2023-08-01T09:43:07Z
date_published: 2016-06-17T00:00:00Z
date_updated: 2024-10-14T12:16:44Z
day: '17'
doi: 10.1002/cphc.201600480
extern: '1'
fulldoi: https://doi.org/10.1002/cphc.201600480
intvolume: ' 17'
issue: '12'
keyword:
- Physical and Theoretical Chemistry
- Atomic and Molecular Physics
- and Optics
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1002/cphc.201600480
month: '06'
oa: 1
oa_version: Published Version
page: 1711-1711
publication: ChemPhysChem
publication_identifier:
eissn:
- 1439-7641
issn:
- 1439-4235
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: 'Inside cover: Reversible photoisomerization of spiropyran on the surfaces
of Au25 nanoclusters (ChemPhysChem 12/2016)'
type: other_academic_publication
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2016'
...
---
_id: '13389'
abstract:
- lang: eng
text: Au25 nanoclusters functionalized with a spiropyran molecular switch are synthesized
via a ligand-exchange reaction at low temperature. The resulting nanoclusters
are characterized by optical and NMR spectroscopies as well as by mass spectrometry.
Spiropyran bound to nanoclusters isomerizes in a reversible fashion when exposed
to UV and visible light, and its properties are similar to those of free spiropyran
molecules in solution. The reversible photoisomerization entails the modulation
of fluorescence as well as the light-controlled self-assembly of nanoclusters.
article_processing_charge: No
article_type: original
author:
- first_name: T.
full_name: Udayabhaskararao, T.
last_name: Udayabhaskararao
- first_name: Pintu K.
full_name: Kundu, Pintu K.
last_name: Kundu
- first_name: Johannes
full_name: Ahrens, Johannes
last_name: Ahrens
- first_name: Rafal
full_name: Klajn, Rafal
id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b
last_name: Klajn
citation:
ama: Udayabhaskararao T, Kundu PK, Ahrens J, Klajn R. Reversible photoisomerization
of spiropyran on the surfaces of Au25 nanoclusters. ChemPhysChem. 2016;17(12):1805-1809.
doi:10.1002/cphc.201500897
apa: Udayabhaskararao, T., Kundu, P. K., Ahrens, J., & Klajn, R. (2016). Reversible
photoisomerization of spiropyran on the surfaces of Au25 nanoclusters. ChemPhysChem.
Wiley. https://doi.org/10.1002/cphc.201500897
chicago: Udayabhaskararao, T., Pintu K. Kundu, Johannes Ahrens, and Rafal Klajn.
“Reversible Photoisomerization of Spiropyran on the Surfaces of Au25 Nanoclusters.”
ChemPhysChem. Wiley, 2016. https://doi.org/10.1002/cphc.201500897.
ieee: T. Udayabhaskararao, P. K. Kundu, J. Ahrens, and R. Klajn, “Reversible photoisomerization
of spiropyran on the surfaces of Au25 nanoclusters,” ChemPhysChem, vol.
17, no. 12. Wiley, pp. 1805–1809, 2016.
ista: Udayabhaskararao T, Kundu PK, Ahrens J, Klajn R. 2016. Reversible photoisomerization
of spiropyran on the surfaces of Au25 nanoclusters. ChemPhysChem. 17(12), 1805–1809.
mla: Udayabhaskararao, T., et al. “Reversible Photoisomerization of Spiropyran on
the Surfaces of Au25 Nanoclusters.” ChemPhysChem, vol. 17, no. 12, Wiley,
2016, pp. 1805–09, doi:10.1002/cphc.201500897.
short: T. Udayabhaskararao, P.K. Kundu, J. Ahrens, R. Klajn, ChemPhysChem 17 (2016)
1805–1809.
date_created: 2023-08-01T09:43:18Z
date_published: 2016-06-17T00:00:00Z
date_updated: 2024-10-14T12:16:54Z
day: '17'
doi: 10.1002/cphc.201500897
extern: '1'
external_id:
pmid:
- '26593975'
fulldoi: https://doi.org/10.1002/cphc.201500897
intvolume: ' 17'
issue: '12'
keyword:
- Physical and Theoretical Chemistry
- Atomic and Molecular Physics
- and Optics
language:
- iso: eng
month: '06'
oa_version: None
page: 1805-1809
pmid: 1
publication: ChemPhysChem
publication_identifier:
eissn:
- 1439-7641
issn:
- 1439-4235
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Reversible photoisomerization of spiropyran on the surfaces of Au25 nanoclusters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2016'
...
---
OA_type: closed access
_id: '1058'
abstract:
- lang: eng
text: Diffraction-unlimited far-field super-resolution fluorescence (nanoscopy)
methods typically rely on transiently transferring fluorophores between two states,
whereby this transfer is usually laid out as a switch. However, depending on whether
this is induced in a spatially controlled manner using a pattern of light (coordinate-targeted)
or stochastically on a single-molecule basis, specific requirements on the fluorophores
are imposed. Therefore, the fluorophores are usually utilized just for one class
of methods only. In this study we demonstrate that the reversibly switchable fluorescent
protein Dreiklang enables live-cell recordings in both spatially controlled and
stochastic modes. We show that the Dreiklang chromophore entails three different
light-induced switching mechanisms, namely a reversible photochemical one, off-switching
by stimulated emission, and a reversible transfer to a long-lived dark state from
the S1 state, all of which can be utilized to overcome the diffraction barrier.
We also find that for the single-molecule- based stochastic GSDIM approach (ground-state
depletion followed by individual molecule return), Dreiklang provides a larger
number of on-off localization events as compared to its progenitor Citrine. Altogether,
Dreiklang is a versatile probe for essentially all popular forms of live-cell
fluorescence nanoscopy.
article_processing_charge: No
article_type: original
author:
- first_name: Nickels
full_name: Jensen, Nickels
last_name: Jensen
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
- first_name: Katrin
full_name: Willig, Katrin
last_name: Willig
- first_name: Flavie
full_name: Lavoie Cardinal, Flavie
last_name: Lavoie Cardinal
- first_name: Tanja
full_name: Brakemann, Tanja
last_name: Brakemann
- first_name: Stefan
full_name: Hell, Stefan
last_name: Hell
- first_name: Stefan
full_name: Jakobs, Stefan
last_name: Jakobs
citation:
ama: Jensen N, Danzl JG, Willig K, et al. Coordinate-targeted and coordinate-stochastic
super-resolution microscopy with the reversibly switchable fluorescent protein
dreiklang. ChemPhysChem. 2014;15(4):756-762. doi:10.1002/cphc.201301034
apa: Jensen, N., Danzl, J. G., Willig, K., Lavoie Cardinal, F., Brakemann, T., Hell,
S., & Jakobs, S. (2014). Coordinate-targeted and coordinate-stochastic super-resolution
microscopy with the reversibly switchable fluorescent protein dreiklang. ChemPhysChem.
Wiley-Blackwell. https://doi.org/10.1002/cphc.201301034
chicago: Jensen, Nickels, Johann G Danzl, Katrin Willig, Flavie Lavoie Cardinal,
Tanja Brakemann, Stefan Hell, and Stefan Jakobs. “Coordinate-Targeted and Coordinate-Stochastic
Super-Resolution Microscopy with the Reversibly Switchable Fluorescent Protein
Dreiklang.” ChemPhysChem. Wiley-Blackwell, 2014. https://doi.org/10.1002/cphc.201301034.
ieee: N. Jensen et al., “Coordinate-targeted and coordinate-stochastic super-resolution
microscopy with the reversibly switchable fluorescent protein dreiklang,” ChemPhysChem,
vol. 15, no. 4. Wiley-Blackwell, pp. 756–762, 2014.
ista: Jensen N, Danzl JG, Willig K, Lavoie Cardinal F, Brakemann T, Hell S, Jakobs
S. 2014. Coordinate-targeted and coordinate-stochastic super-resolution microscopy
with the reversibly switchable fluorescent protein dreiklang. ChemPhysChem. 15(4),
756–762.
mla: Jensen, Nickels, et al. “Coordinate-Targeted and Coordinate-Stochastic Super-Resolution
Microscopy with the Reversibly Switchable Fluorescent Protein Dreiklang.” ChemPhysChem,
vol. 15, no. 4, Wiley-Blackwell, 2014, pp. 756–62, doi:10.1002/cphc.201301034.
short: N. Jensen, J.G. Danzl, K. Willig, F. Lavoie Cardinal, T. Brakemann, S. Hell,
S. Jakobs, ChemPhysChem 15 (2014) 756–762.
date_created: 2018-12-11T11:49:55Z
date_published: 2014-03-17T00:00:00Z
date_updated: 2026-09-04T12:18:04Z
day: '17'
doi: 10.1002/cphc.201301034
extern: '1'
external_id:
pmid:
- '24497300'
fulldoi: https://doi.org/10.1002/cphc.201301034
intvolume: ' 15'
issue: '4'
language:
- iso: eng
month: '03'
oa_version: None
page: 756 - 762
pmid: 1
publication: ChemPhysChem
publication_identifier:
eissn:
- 1439-7641
issn:
- 1439-4235
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6332'
status: public
title: Coordinate-targeted and coordinate-stochastic super-resolution microscopy with
the reversibly switchable fluorescent protein dreiklang
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2014'
...