[{"doi":"10.1093/jxb/eraf084","scopus_import":"1","publication":"Journal of Experimental Botany","date_published":"2025-07-02T00:00:00Z","language":[{"iso":"eng"}],"type":"journal_article","acknowledgement":"This research was supported by FONDECYT grants 1170950 and 1211311 and by ANID PhD fellowship 2020-21201663 to PhD student CO-N. The microscopes used in this work were funded by grants FONDEQUIP #EQM 140019 and #EQM12-0003 at the Advanced Microscopy Unit of the Biology Department, Faculty of Science, University of Chile.\r\nWe thank Jiri Friml for donating the XVE»AUXILIN-LIKE2 (AX2) line to support our research. We wish to acknowledge the active and helpful discussion of all the members of the LNM team and the Plant Molecular Biology Centre at Universidad de Chile.","issue":"10","year":"2025","intvolume":"        76","title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"pmid":1,"status":"public","article_processing_charge":"No","abstract":[{"text":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which has not been well described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference with clathrin-mediated endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-trans-Golgi network (TGN)/early endosome (EE) trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during vacuole configuration, most probably carrying the material that allows the establishment of the vacuole in elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional players must be defined.","lang":"eng"}],"month":"07","volume":76,"isi":1,"department":[{"_id":"JiFr"}],"date_created":"2025-07-20T22:02:01Z","article_type":"original","oa_version":"None","citation":{"ista":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. 2025. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 76(10), 2700–2714.","ieee":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, and L. Norambuena, “The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana,” <i>Journal of Experimental Botany</i>, vol. 76, no. 10. Oxford University Press, pp. 2700–2714, 2025.","chicago":"Osorio-Navarro, Claudio, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, and Lorena Norambuena. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/jxb/eraf084\">https://doi.org/10.1093/jxb/eraf084</a>.","mla":"Osorio-Navarro, Claudio, et al. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” <i>Journal of Experimental Botany</i>, vol. 76, no. 10, Oxford University Press, 2025, pp. 2700–14, doi:<a href=\"https://doi.org/10.1093/jxb/eraf084\">10.1093/jxb/eraf084</a>.","apa":"Osorio-Navarro, C., Neira-Valenzuela, G., Sierra, P., Adamowski, M., Toledo, J., &#38; Norambuena, L. (2025). The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraf084\">https://doi.org/10.1093/jxb/eraf084</a>","short":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, L. Norambuena, Journal of Experimental Botany 76 (2025) 2700–2714.","ama":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. <i>Journal of Experimental Botany</i>. 2025;76(10):2700-2714. doi:<a href=\"https://doi.org/10.1093/jxb/eraf084\">10.1093/jxb/eraf084</a>"},"date_updated":"2025-09-30T14:04:16Z","page":"2700-2714","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"isi":["001482869200001"],"pmid":["40056424"]},"OA_type":"closed access","day":"02","publication_status":"published","quality_controlled":"1","_id":"20031","author":[{"first_name":"Claudio","full_name":"Osorio-Navarro, Claudio","last_name":"Osorio-Navarro"},{"last_name":"Neira-Valenzuela","full_name":"Neira-Valenzuela, Gabriel","first_name":"Gabriel"},{"full_name":"Sierra, Paula","last_name":"Sierra","first_name":"Paula"},{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","first_name":"Maciek","last_name":"Adamowski","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek"},{"last_name":"Toledo","full_name":"Toledo, Jorge","first_name":"Jorge"},{"first_name":"Lorena","last_name":"Norambuena","full_name":"Norambuena, Lorena"}],"publisher":"Oxford University Press"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","date_created":"2023-12-24T23:00:53Z","citation":{"ama":"Del Bianco M, Friml J, Strader L, Kepinski S. Auxin research: Creating tools for a greener future. <i>Journal of Experimental Botany</i>. 2023;74(22):6889-6892. doi:<a href=\"https://doi.org/10.1093/jxb/erad420\">10.1093/jxb/erad420</a>","short":"M. Del Bianco, J. Friml, L. Strader, S. Kepinski, Journal of Experimental Botany 74 (2023) 6889–6892.","apa":"Del Bianco, M., Friml, J., Strader, L., &#38; Kepinski, S. (2023). Auxin research: Creating tools for a greener future. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/erad420\">https://doi.org/10.1093/jxb/erad420</a>","mla":"Del Bianco, Marta, et al. “Auxin Research: Creating Tools for a Greener Future.” <i>Journal of Experimental Botany</i>, vol. 74, no. 22, Oxford University Press, 2023, pp. 6889–92, doi:<a href=\"https://doi.org/10.1093/jxb/erad420\">10.1093/jxb/erad420</a>.","chicago":"Del Bianco, Marta, Jiří Friml, Lucia Strader, and Stefan Kepinski. “Auxin Research: Creating Tools for a Greener Future.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/jxb/erad420\">https://doi.org/10.1093/jxb/erad420</a>.","ista":"Del Bianco M, Friml J, Strader L, Kepinski S. 2023. Auxin research: Creating tools for a greener future. Journal of Experimental Botany. 74(22), 6889–6892.","ieee":"M. Del Bianco, J. Friml, L. Strader, and S. Kepinski, “Auxin research: Creating tools for a greener future,” <i>Journal of Experimental Botany</i>, vol. 74, no. 22. Oxford University Press, pp. 6889–6892, 2023."},"oa_version":"Published Version","file":[{"date_updated":"2024-01-02T09:23:57Z","file_size":425194,"file_name":"2023_JourExperimentalBotany_DelBianco.pdf","content_type":"application/pdf","success":1,"checksum":"f66fb960fd791dea53fd0e087f2fbbe8","relation":"main_file","creator":"dernst","date_created":"2024-01-02T09:23:57Z","file_id":"14724","access_level":"open_access"}],"status":"public","department":[{"_id":"JiFr"}],"article_processing_charge":"Yes (in subscription journal)","month":"12","abstract":[{"text":"Amid the delays due to the global pandemic, in early October 2022, the auxin community gathered in the idyllic peninsula of Cavtat, Croatia. More than 170 scientists from across the world converged to discuss the latest advancements in fundamental and applied research in the field. The topics, from signalling and transport to plant architecture and response to the environment, show how auxin research must bridge from the molecular realm to macroscopic developmental responses. This is mirrored in this collection of reviews, contributed by participants of the Auxin 2022 meeting.","lang":"eng"}],"volume":74,"isi":1,"year":"2023","intvolume":"        74","issue":"22","publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"pmid":1,"title":"Auxin research: Creating tools for a greener future","doi":"10.1093/jxb/erad420","type":"journal_article","scopus_import":"1","language":[{"iso":"eng"}],"date_published":"2023-12-01T00:00:00Z","publication":"Journal of Experimental Botany","oa":1,"file_date_updated":"2024-01-02T09:23:57Z","publisher":"Oxford University Press","_id":"14709","author":[{"first_name":"Marta","full_name":"Del Bianco, Marta","last_name":"Del Bianco"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml"},{"first_name":"Lucia","last_name":"Strader","full_name":"Strader, Lucia"},{"first_name":"Stefan","last_name":"Kepinski","full_name":"Kepinski, Stefan"}],"ddc":["580"],"has_accepted_license":"1","publication_status":"published","quality_controlled":"1","day":"01","external_id":{"pmid":["38038239"],"isi":["001145889700001"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"6889-6892","date_updated":"2025-09-09T14:06:39Z"},{"date_published":"2022-04-18T00:00:00Z","publication":"Journal of Experimental Botany","language":[{"iso":"eng"}],"scopus_import":"1","type":"journal_article","doi":"10.1093/jxb/erac019","title":"Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots","publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"pmid":1,"article_number":"erac019","issue":"8","acknowledgement":"We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A. University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC for providing seeds. We thank Veronique Storme for help with the statistical analyses. Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility established by the University of Milan.\r\nThis work was supported by grants of the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek (FWO) to TB and (G002220N) SV; the special research fund of Ghent University to EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985 to JF, and by a PhD fellowship from the University of Milan to MG.","intvolume":"        73","year":"2022","month":"04","isi":1,"volume":73,"abstract":[{"lang":"eng","text":"Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven to be instrumental to reveal the various faces of auxin, they display in some cases distinct bioactivities compared to IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive endosomal aggregations (BFA bodies), and the correlation with the ability to elicit Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots."}],"article_processing_charge":"No","department":[{"_id":"JiFr"}],"status":"public","oa_version":"Submitted Version","citation":{"ama":"Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. 2022;73(8). doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>","short":"R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher, M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman, J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).","ieee":"R. Wang <i>et al.</i>, “Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots,” <i>Journal of Experimental Botany</i>, vol. 73, no. 8. Oxford University Press, 2022.","ista":"Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany. 73(8), erac019.","apa":"Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste, S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>","mla":"Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>, vol. 73, no. 8, erac019, Oxford University Press, 2022, doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>.","chicago":"Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher, et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>."},"ec_funded":1,"date_created":"2022-02-03T09:19:01Z","article_type":"original","main_file_link":[{"url":"https://biblio.ugent.be/publication/8738721","open_access":"1"}],"date_updated":"2025-05-14T11:06:37Z","project":[{"call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"isi":["000764220900001"],"pmid":["35085386"]},"day":"18","quality_controlled":"1","publication_status":"published","author":[{"full_name":"Wang, R","last_name":"Wang","first_name":"R"},{"last_name":"Himschoot","full_name":"Himschoot, E","first_name":"E"},{"first_name":"M","full_name":"Grenzi, M","last_name":"Grenzi"},{"first_name":"J","full_name":"Chen, J","last_name":"Chen"},{"first_name":"A","full_name":"Safi, A","last_name":"Safi"},{"last_name":"Krebs","full_name":"Krebs, M","first_name":"M"},{"first_name":"K","full_name":"Schumacher, K","last_name":"Schumacher"},{"first_name":"MK","full_name":"Nowack, MK","last_name":"Nowack"},{"full_name":"Moeder, W","last_name":"Moeder","first_name":"W"},{"first_name":"K","full_name":"Yoshioka, K","last_name":"Yoshioka"},{"last_name":"Van Damme","full_name":"Van Damme, D","first_name":"D"},{"full_name":"De Smet, I","last_name":"De Smet","first_name":"I"},{"last_name":"Geelen","full_name":"Geelen, D","first_name":"D"},{"full_name":"Beeckman, T","last_name":"Beeckman","first_name":"T"},{"full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"},{"last_name":"Costa","full_name":"Costa, A","first_name":"A"},{"first_name":"S","full_name":"Vanneste, S","last_name":"Vanneste"}],"_id":"10717","publisher":"Oxford University Press","oa":1},{"day":"06","external_id":{"pmid":["32179893"],"isi":["000553125400007"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","page":"3986–3998","date_updated":"2024-10-21T06:02:26Z","oa":1,"file_date_updated":"2020-10-06T07:41:35Z","publisher":"Oxford University Press","has_accepted_license":"1","ddc":["580"],"author":[{"full_name":"Lee, E","last_name":"Lee","first_name":"E"},{"full_name":"Vila Nova Santana, B","last_name":"Vila Nova Santana","first_name":"B"},{"full_name":"Samuels, E","last_name":"Samuels","first_name":"E"},{"last_name":"Benitez-Fuente","full_name":"Benitez-Fuente, F","first_name":"F"},{"first_name":"E","last_name":"Corsi","full_name":"Corsi, E"},{"first_name":"MA","full_name":"Botella, MA","last_name":"Botella"},{"full_name":"Perez-Sancho, J","last_name":"Perez-Sancho","first_name":"J"},{"last_name":"Vanneste","full_name":"Vanneste, S","first_name":"S"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"A","full_name":"Macho, A","last_name":"Macho"},{"full_name":"Alves Azevedo, A","last_name":"Alves Azevedo","first_name":"A"},{"first_name":"A","last_name":"Rosado","full_name":"Rosado, A"}],"_id":"7646","publication_status":"published","quality_controlled":"1","intvolume":"        71","year":"2020","issue":"14","pmid":1,"publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"title":"Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis","doi":"10.1093/jxb/eraa138","type":"journal_article","language":[{"iso":"eng"}],"publication":"Journal of Experimental Botany","date_published":"2020-07-06T00:00:00Z","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"date_created":"2020-04-06T10:57:08Z","article_type":"original","citation":{"ama":"Lee E, Vila Nova Santana B, Samuels E, et al. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. 2020;71(14):3986–3998. doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>","short":"E. Lee, B. Vila Nova Santana, E. Samuels, F. Benitez-Fuente, E. Corsi, M. Botella, J. Perez-Sancho, S. Vanneste, J. Friml, A. Macho, A. Alves Azevedo, A. Rosado, Journal of Experimental Botany 71 (2020) 3986–3998.","ieee":"E. Lee <i>et al.</i>, “Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 71, no. 14. Oxford University Press, pp. 3986–3998, 2020.","ista":"Lee E, Vila Nova Santana B, Samuels E, Benitez-Fuente F, Corsi E, Botella M, Perez-Sancho J, Vanneste S, Friml J, Macho A, Alves Azevedo A, Rosado A. 2020. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. Journal of Experimental Botany. 71(14), 3986–3998.","mla":"Lee, E., et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 71, no. 14, Oxford University Press, 2020, pp. 3986–3998, doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>.","apa":"Lee, E., Vila Nova Santana, B., Samuels, E., Benitez-Fuente, F., Corsi, E., Botella, M., … Rosado, A. (2020). Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>","chicago":"Lee, E, B Vila Nova Santana, E Samuels, F Benitez-Fuente, E Corsi, MA Botella, J Perez-Sancho, et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>."},"file":[{"success":1,"checksum":"b06aaaa93dc41896da805fe4b75cf3a1","content_type":"application/pdf","file_size":1916031,"date_updated":"2020-10-06T07:41:35Z","file_name":"2020_JourExperimBotany_Lee.pdf","access_level":"open_access","creator":"dernst","date_created":"2020-10-06T07:41:35Z","relation":"main_file","file_id":"8613"}],"oa_version":"Published Version","status":"public","department":[{"_id":"JiFr"}],"volume":71,"abstract":[{"text":"In plant cells, environmental stressors promote changes in connectivity between the cortical ER and the PM. Although this process is tightly regulated in space and time, the molecular signals and structural components mediating these changes in inter-organelle communication are only starting to be characterized. In this report, we confirm the presence of a putative tethering complex containing the synaptotagmins 1 and 5 (SYT1 and SYT5) and the Ca2+ and lipid binding protein 1 (CLB1/SYT7). This complex is enriched at ER-PM contact sites (EPCS), have slow responses to changes in extracellular Ca2+, and display severe cytoskeleton-dependent rearrangements in response to the trivalent lanthanum (La3+) and gadolinium (Gd3+) rare earth elements (REEs). Although REEs are generally used as non-selective cation channel blockers at the PM, here we show that the slow internalization of REEs into the cytosol underlies the activation of the Ca2+/Calmodulin intracellular signaling, the accumulation of phosphatidylinositol-4-phosphate (PI4P) at the PM, and the cytoskeleton-dependent rearrangement of the SYT1/SYT5 EPCS complexes. We propose that the observed EPCS rearrangements act as a slow adaptive response to sustained stress conditions, and that this process involves the accumulation of stress-specific phosphoinositides species at the PM.","lang":"eng"}],"isi":1,"month":"07","article_processing_charge":"No"},{"issue":"15","year":"2020","intvolume":"        71","title":"The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate","pmid":1,"publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"doi":"10.1093/jxb/eraa242","scopus_import":"1","language":[{"iso":"eng"}],"date_published":"2020-07-25T00:00:00Z","publication":"Journal of Experimental Botany","type":"journal_article","article_type":"original","date_created":"2020-06-08T10:10:28Z","oa_version":"Submitted Version","citation":{"mla":"Maghiaoui, A., et al. “The Arabidopsis NRT1.1 Transceptor Coordinately Controls Auxin Biosynthesis and Transport to Regulate Root Branching in Response to Nitrate.” <i>Journal of Experimental Botany</i>, vol. 71, no. 15, Oxford University Press, 2020, pp. 4480–94, doi:<a href=\"https://doi.org/10.1093/jxb/eraa242\">10.1093/jxb/eraa242</a>.","apa":"Maghiaoui, A., Bouguyon, E., Cuesta, C., Perrine-Walker, F., Alcon, C., Krouk, G., … Bach, L. (2020). The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa242\">https://doi.org/10.1093/jxb/eraa242</a>","chicago":"Maghiaoui, A, E Bouguyon, Candela Cuesta, F Perrine-Walker, C Alcon, G Krouk, Eva Benková, P Nacry, A Gojon, and L Bach. “The Arabidopsis NRT1.1 Transceptor Coordinately Controls Auxin Biosynthesis and Transport to Regulate Root Branching in Response to Nitrate.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa242\">https://doi.org/10.1093/jxb/eraa242</a>.","ieee":"A. Maghiaoui <i>et al.</i>, “The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate,” <i>Journal of Experimental Botany</i>, vol. 71, no. 15. Oxford University Press, pp. 4480–4494, 2020.","ista":"Maghiaoui A, Bouguyon E, Cuesta C, Perrine-Walker F, Alcon C, Krouk G, Benková E, Nacry P, Gojon A, Bach L. 2020. The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. Journal of Experimental Botany. 71(15), 4480–4494.","ama":"Maghiaoui A, Bouguyon E, Cuesta C, et al. The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. <i>Journal of Experimental Botany</i>. 2020;71(15):4480-4494. doi:<a href=\"https://doi.org/10.1093/jxb/eraa242\">10.1093/jxb/eraa242</a>","short":"A. Maghiaoui, E. Bouguyon, C. Cuesta, F. Perrine-Walker, C. Alcon, G. Krouk, E. Benková, P. Nacry, A. Gojon, L. Bach, Journal of Experimental Botany 71 (2020) 4480–4494."},"status":"public","article_processing_charge":"No","month":"07","isi":1,"volume":71,"abstract":[{"lang":"eng","text":"In agricultural systems, nitrate is the main source of nitrogen available for plants. Besides its role as a nutrient, nitrate has been shown to act as a signal molecule for plant growth, development and stress responses. In Arabidopsis, the NRT1.1 nitrate transceptor represses lateral root (LR) development at low nitrate availability by promoting auxin basipetal transport out of the LR primordia (LRPs). In addition, our present study shows that NRT1.1 acts as a negative regulator of the TAR2 auxin biosynthetic gene expression in the root stele. This is expected to repress local auxin biosynthesis and thus to reduce acropetal auxin supply to the LRPs. Moreover, NRT1.1 also negatively affects expression of the LAX3 auxin influx carrier, thus preventing cell wall remodeling required for overlying tissues separation during LRP emergence. Both NRT1.1-mediated repression of TAR2 and LAX3 are suppressed at high nitrate availability, resulting in the nitrate induction of TAR2 and LAX3 expression that is required for optimal stimulation of LR development by nitrate. Altogether, our results indicate that the NRT1.1 transceptor coordinately controls several crucial auxin-associated processes required for LRP development, and as a consequence that NRT1.1 plays a much more integrated role than previously anticipated in regulating the nitrate response of root system architecture."}],"department":[{"_id":"EvBe"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","day":"25","external_id":{"isi":["000553127600013"],"pmid":["32428238"]},"date_updated":"2024-10-21T06:02:27Z","page":"4480-4494","main_file_link":[{"url":"https://hal.inrae.fr/hal-02619371","open_access":"1"}],"oa":1,"author":[{"full_name":"Maghiaoui, A","last_name":"Maghiaoui","first_name":"A"},{"first_name":"E","full_name":"Bouguyon, E","last_name":"Bouguyon"},{"full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela"},{"last_name":"Perrine-Walker","full_name":"Perrine-Walker, F","first_name":"F"},{"first_name":"C","last_name":"Alcon","full_name":"Alcon, C"},{"first_name":"G","last_name":"Krouk","full_name":"Krouk, G"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"},{"first_name":"P","full_name":"Nacry, P","last_name":"Nacry"},{"first_name":"A","full_name":"Gojon, A","last_name":"Gojon"},{"last_name":"Bach","full_name":"Bach, L","first_name":"L"}],"_id":"7948","publisher":"Oxford University Press","publication_status":"published","quality_controlled":"1"},{"page":"2367-2378","date_updated":"2025-04-15T07:48:01Z","external_id":{"pmid":["29538714"],"isi":["000430727000016"]},"day":"13","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","project":[{"grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"keyword":["Plant Science","Physiology"],"publication_status":"published","quality_controlled":"1","publisher":"Oxford University Press","author":[{"first_name":"Taraka Ramji","full_name":"Moturu, Taraka Ramji","last_name":"Moturu"},{"first_name":"Sravankumar","last_name":"Thula","full_name":"Thula, Sravankumar"},{"last_name":"Singh","full_name":"Singh, Ravi Kumar","first_name":"Ravi Kumar"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"full_name":"Vařeková, Radka Svobodová","last_name":"Vařeková","first_name":"Radka Svobodová"},{"full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sibu","full_name":"Simon, Sibu","last_name":"Simon"}],"_id":"10881","doi":"10.1093/jxb/ery097","type":"journal_article","scopus_import":"1","publication":"Journal of Experimental Botany","date_published":"2018-04-13T00:00:00Z","language":[{"iso":"eng"}],"year":"2018","intvolume":"        69","acknowledgement":"This project received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Actions and it is co-financed by the South Moravian Region under grant agreement No. 665860 (SS). Access to computing and storage facilities owned by parties and projects contributing to the national grid infrastructure, MetaCentrum, provided under the program ‘Projects of Large Infrastructure for Research, Development, and Innovations’ (LM2010005) was greatly appreciated (RSV). The project was funded by The Ministry of Education, Youth and Sports/MES of the Czech Republic under the project CEITEC 2020 (LQ1601) (TN, TRM). JF was supported by the European Research Council (project ERC-2011-StG 20101109-PSDP) and the Czech Science Foundation GAČR (GA13-40637S). We thank Dr Kamel Chibani for active discussions on the evolutionary analysis and Nandan Mysore Vardarajan for his critical comments on the manuscript. This article reflects\r\nonly the authors’ views, and the EU is not responsible for any use that may be made of the information it contains. ","issue":"9","pmid":1,"publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"title":"Molecular evolution and diversification of the SMXL gene family","status":"public","department":[{"_id":"JiFr"}],"article_processing_charge":"No","isi":1,"month":"04","volume":69,"abstract":[{"lang":"eng","text":"Strigolactones (SLs) are a relatively recent addition to the list of plant hormones that control different aspects of plant development. SL signalling is perceived by an α/β hydrolase, DWARF 14 (D14). A close homolog of D14, KARRIKIN INSENSTIVE2 (KAI2), is involved in perception of an uncharacterized molecule called karrikin (KAR). Recent studies in Arabidopsis identified the SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-LIKE 7 (SMXL7) to be potential SCF–MAX2 complex-mediated proteasome targets of KAI2 and D14, respectively. Genetic studies on SMXL7 and SMAX1 demonstrated distinct developmental roles for each, but very little is known about these repressors in terms of their sequence features. In this study, we performed an extensive comparative analysis of SMXLs and determined their phylogenetic and evolutionary history in the plant lineage. Our results show that SMXL family members can be sub-divided into four distinct phylogenetic clades/classes, with an ancient SMAX1. Further, we identified the clade-specific motifs that have evolved and that might act as determinants of SL-KAR signalling specificity. These specificities resulted from functional diversities among the clades. Our results suggest that a gradual co-evolution of SMXL members with their upstream receptors D14/KAI2 provided an increased specificity to both the SL perception and response in land plants."}],"ec_funded":1,"article_type":"original","date_created":"2022-03-18T12:43:22Z","citation":{"short":"T.R. Moturu, S. Thula, R.K. Singh, T. Nodzyński, R.S. Vařeková, J. Friml, S. Simon, Journal of Experimental Botany 69 (2018) 2367–2378.","ama":"Moturu TR, Thula S, Singh RK, et al. Molecular evolution and diversification of the SMXL gene family. <i>Journal of Experimental Botany</i>. 2018;69(9):2367-2378. doi:<a href=\"https://doi.org/10.1093/jxb/ery097\">10.1093/jxb/ery097</a>","chicago":"Moturu, Taraka Ramji, Sravankumar Thula, Ravi Kumar Singh, Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular Evolution and Diversification of the SMXL Gene Family.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1093/jxb/ery097\">https://doi.org/10.1093/jxb/ery097</a>.","mla":"Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of the SMXL Gene Family.” <i>Journal of Experimental Botany</i>, vol. 69, no. 9, Oxford University Press, 2018, pp. 2367–78, doi:<a href=\"https://doi.org/10.1093/jxb/ery097\">10.1093/jxb/ery097</a>.","apa":"Moturu, T. R., Thula, S., Singh, R. K., Nodzyński, T., Vařeková, R. S., Friml, J., &#38; Simon, S. (2018). Molecular evolution and diversification of the SMXL gene family. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/ery097\">https://doi.org/10.1093/jxb/ery097</a>","ista":"Moturu TR, Thula S, Singh RK, Nodzyński T, Vařeková RS, Friml J, Simon S. 2018. Molecular evolution and diversification of the SMXL gene family. Journal of Experimental Botany. 69(9), 2367–2378.","ieee":"T. R. Moturu <i>et al.</i>, “Molecular evolution and diversification of the SMXL gene family,” <i>Journal of Experimental Botany</i>, vol. 69, no. 9. Oxford University Press, pp. 2367–2378, 2018."},"oa_version":"None"}]