---
_id: '7005'
abstract:
- lang: eng
  text: Activity-dependent bulk endocytosis generates synaptic vesicles (SVs) during
    intense neuronal activity via a two-step process. First, bulk endosomes are formed
    direct from the plasma membrane from which SVs are then generated. SV generation
    from bulk endosomes requires the efflux of previously accumulated calcium and
    activation of the protein phosphatase calcineurin. However, it is still unknown
    how calcineurin mediates SV generation. We addressed this question using a series
    of acute interventions that decoupled the generation of SVs from bulk endosomes
    in rat primary neuronal culture. This was achieved by either disruption of protein–protein
    interactions via delivery of competitive peptides, or inhibition of enzyme activity
    by known inhibitors. SV generation was monitored using either a morphological
    horseradish peroxidase assay or an optical assay that monitors the replenishment
    of the reserve SV pool. We found that SV generation was inhibited by, (i) peptides
    that disrupt calcineurin interactions, (ii) an inhibitor of dynamin I GTPase activity
    and (iii) peptides that disrupt the phosphorylation-dependent dynamin I–syndapin
    I interaction. Peptides that disrupted syndapin I interactions with eps15 homology
    domain-containing proteins had no effect. This revealed that (i) calcineurin must
    be localized at bulk endosomes to mediate its effect, (ii) dynamin I GTPase activity
    is essential for SV fission and (iii) the calcineurin-dependent interaction between
    dynamin I and syndapin I is essential for SV generation. We therefore propose
    that a calcineurin-dependent dephosphorylation cascade that requires both dynamin
    I GTPase and syndapin I lipid-deforming activity is essential for SV generation
    from bulk endosomes.
article_processing_charge: No
article_type: original
author:
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Michael A.
  full_name: Cousin, Michael A.
  last_name: Cousin
citation:
  ama: Cheung GT, Cousin MA. Synaptic vesicle generation from activity‐dependent bulk
    endosomes requires a dephosphorylation‐dependent dynamin–syndapin interaction.
    <i>Journal of Neurochemistry</i>. 2019;151(5):570-583. doi:<a href="https://doi.org/10.1111/jnc.14862">10.1111/jnc.14862</a>
  apa: Cheung, G. T., &#38; Cousin, M. A. (2019). Synaptic vesicle generation from
    activity‐dependent bulk endosomes requires a dephosphorylation‐dependent dynamin–syndapin
    interaction. <i>Journal of Neurochemistry</i>. Wiley. <a href="https://doi.org/10.1111/jnc.14862">https://doi.org/10.1111/jnc.14862</a>
  chicago: Cheung, Giselle T, and Michael A. Cousin. “Synaptic Vesicle Generation
    from Activity‐dependent Bulk Endosomes Requires a Dephosphorylation‐dependent
    Dynamin–Syndapin Interaction.” <i>Journal of Neurochemistry</i>. Wiley, 2019.
    <a href="https://doi.org/10.1111/jnc.14862">https://doi.org/10.1111/jnc.14862</a>.
  ieee: G. T. Cheung and M. A. Cousin, “Synaptic vesicle generation from activity‐dependent
    bulk endosomes requires a dephosphorylation‐dependent dynamin–syndapin interaction,”
    <i>Journal of Neurochemistry</i>, vol. 151, no. 5. Wiley, pp. 570–583, 2019.
  ista: Cheung GT, Cousin MA. 2019. Synaptic vesicle generation from activity‐dependent
    bulk endosomes requires a dephosphorylation‐dependent dynamin–syndapin interaction.
    Journal of Neurochemistry. 151(5), 570–583.
  mla: Cheung, Giselle T., and Michael A. Cousin. “Synaptic Vesicle Generation from
    Activity‐dependent Bulk Endosomes Requires a Dephosphorylation‐dependent Dynamin–Syndapin
    Interaction.” <i>Journal of Neurochemistry</i>, vol. 151, no. 5, Wiley, 2019,
    pp. 570–83, doi:<a href="https://doi.org/10.1111/jnc.14862">10.1111/jnc.14862</a>.
  short: G.T. Cheung, M.A. Cousin, Journal of Neurochemistry 151 (2019) 570–583.
date_created: 2019-11-12T14:37:08Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-08-30T07:21:50Z
day: '01'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1111/jnc.14862
external_id:
  isi:
  - '000490703100001'
  pmid:
  - '31479508'
file:
- access_level: open_access
  checksum: ec1fb2aebb874009bc309adaada6e1d7
  content_type: application/pdf
  creator: dernst
  date_created: 2020-02-05T10:30:02Z
  date_updated: 2020-07-14T12:47:47Z
  file_id: '7452'
  file_name: 2019_JournNeurochemistry_Cheung.pdf
  file_size: 4334962
  relation: main_file
file_date_updated: 2020-07-14T12:47:47Z
has_accepted_license: '1'
intvolume: '       151'
isi: 1
issue: '5'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 570-583
pmid: 1
publication: Journal of Neurochemistry
publication_identifier:
  eissn:
  - 1471-4159
  issn:
  - 0022-3042
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synaptic vesicle generation from activity‐dependent bulk endosomes requires
  a dephosphorylation‐dependent dynamin–syndapin interaction
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 151
year: '2019'
...
---
OA_type: closed access
_id: '2631'
abstract:
- lang: eng
  text: Cyclic ADP-ribose (cADP-ribose) is a putative second messenger or modulator.
    However, the role of cADP-ribose in the downstream signals of the metabotropic
    glutamate receptors (mGluRs) is unclear. Here, we show that glutamate stimulates
    ADP-ribosyl cyclase activity in rat or mouse crude membranes of retina via group
    III mGluRs or in superior cervical ganglion via group I mGluRs. The retina of
    mGluR6-deficient mice showed no increase in the ADP-ribosyl cyclase level in response
    to glutamate. GTP enhanced the initial rate of basal and glutamate-stimulated
    cyclase activity. GTP-γ-S also stimulated basal activity. To determine whether
    the coupling mode of mGluRs to ADP-ribosyl cyclase is a feature common to individual
    cloned mGluRs, we expressed each mGluR subtype in NG108-15 neuroblastoma x glioma
    hybrid cells. The glutamate-induced stimulation of the cyclase occurs preferentially
    in NG108-15 cells over-expressing mGluRs1, 3, 5, and 6. Cells expressing mGluR2
    or mGluRs4 and 7 exhibit inhibition or no coupling, respectively. Glutamate-induced
    activation or inhibition of the cyclase activity was eliminated after pre-treatment
    with cholera or pertussis toxin, respectively. Thus, the subtype-specific coupling
    of mGluRs to ADP-ribosyl cyclase via G proteins suggests that some glutamate-evoked
    neuronal functions are mediated by cADP-ribose.
article_processing_charge: No
article_type: original
author:
- first_name: Haruhiro
  full_name: Higashida, Haruhiro
  last_name: Higashida
- first_name: Jia
  full_name: Zhang, Jia
  last_name: Zhang
- first_name: Sumiko
  full_name: Mochida, Sumiko
  last_name: Mochida
- first_name: Xiao
  full_name: Chen, Xiao
  last_name: Chen
- first_name: Yeonsook
  full_name: Shin, Yeonsook
  last_name: Shin
- first_name: Mami
  full_name: Noda, Mami
  last_name: Noda
- first_name: Kazi
  full_name: Hossain, Kazi
  last_name: Hossain
- first_name: Naoto
  full_name: Hoshi, Naoto
  last_name: Hoshi
- first_name: Minako
  full_name: Hashii, Minako
  last_name: Hashii
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Shigetada
  full_name: Nakanishi, Shigetada
  last_name: Nakanishi
- first_name: Yutaka
  full_name: Fukuda, Yutaka
  last_name: Fukuda
- first_name: Shigeru
  full_name: Yokoyama, Shigeru
  last_name: Yokoyama
citation:
  ama: Higashida H, Zhang J, Mochida S, et al. Subtype-specific coupling with ADP-ribosyl
    cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion
    and NG108-15 cells. <i>Journal of Neurochemistry</i>. 2003;85(5):1148-1158. doi:<a
    href="https://doi.org/10.1046/j.1471-4159.2003.01751.x">10.1046/j.1471-4159.2003.01751.x</a>
  apa: Higashida, H., Zhang, J., Mochida, S., Chen, X., Shin, Y., Noda, M., … Yokoyama,
    S. (2003). Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic
    glutamate receptors in retina, cervical superior ganglion and NG108-15 cells.
    <i>Journal of Neurochemistry</i>. Wiley-Blackwell. <a href="https://doi.org/10.1046/j.1471-4159.2003.01751.x">https://doi.org/10.1046/j.1471-4159.2003.01751.x</a>
  chicago: Higashida, Haruhiro, Jia Zhang, Sumiko Mochida, Xiao Chen, Yeonsook Shin,
    Mami Noda, Kazi Hossain, et al. “Subtype-Specific Coupling with ADP-Ribosyl Cyclase
    of Metabotropic Glutamate Receptors in Retina, Cervical Superior Ganglion and
    NG108-15 Cells.” <i>Journal of Neurochemistry</i>. Wiley-Blackwell, 2003. <a href="https://doi.org/10.1046/j.1471-4159.2003.01751.x">https://doi.org/10.1046/j.1471-4159.2003.01751.x</a>.
  ieee: H. Higashida <i>et al.</i>, “Subtype-specific coupling with ADP-ribosyl cyclase
    of metabotropic glutamate receptors in retina, cervical superior ganglion and
    NG108-15 cells,” <i>Journal of Neurochemistry</i>, vol. 85, no. 5. Wiley-Blackwell,
    pp. 1148–1158, 2003.
  ista: Higashida H, Zhang J, Mochida S, Chen X, Shin Y, Noda M, Hossain K, Hoshi
    N, Hashii M, Shigemoto R, Nakanishi S, Fukuda Y, Yokoyama S. 2003. Subtype-specific
    coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina,
    cervical superior ganglion and NG108-15 cells. Journal of Neurochemistry. 85(5),
    1148–1158.
  mla: Higashida, Haruhiro, et al. “Subtype-Specific Coupling with ADP-Ribosyl Cyclase
    of Metabotropic Glutamate Receptors in Retina, Cervical Superior Ganglion and
    NG108-15 Cells.” <i>Journal of Neurochemistry</i>, vol. 85, no. 5, Wiley-Blackwell,
    2003, pp. 1148–58, doi:<a href="https://doi.org/10.1046/j.1471-4159.2003.01751.x">10.1046/j.1471-4159.2003.01751.x</a>.
  short: H. Higashida, J. Zhang, S. Mochida, X. Chen, Y. Shin, M. Noda, K. Hossain,
    N. Hoshi, M. Hashii, R. Shigemoto, S. Nakanishi, Y. Fukuda, S. Yokoyama, Journal
    of Neurochemistry 85 (2003) 1148–1158.
date_created: 2018-12-11T11:58:46Z
date_published: 2003-06-01T00:00:00Z
date_updated: 2026-05-22T11:05:45Z
day: '01'
doi: 10.1046/j.1471-4159.2003.01751.x
extern: '1'
external_id:
  pmid:
  - '12753074'
intvolume: '        85'
issue: '5'
language:
- iso: eng
month: '06'
oa_version: None
page: 1148 - 1158
pmid: 1
publication: Journal of Neurochemistry
publication_identifier:
  eissn:
  - 1471-4159
  issn:
  - 0022-3042
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4268'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate
  receptors in retina, cervical superior ganglion and NG108-15 cells
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 85
year: '2003'
...