[{"_id":"19420","quality_controlled":"1","date_updated":"2025-09-30T11:05:55Z","month":"03","doi":"10.1093/pcp/pcaf008","article_processing_charge":"No","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","date_published":"2025-03-05T00:00:00Z","OA_type":"closed access","publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"}],"publisher":"Oxford University Press","ec_funded":1,"isi":1,"publication_status":"published","publication":"Plant and Cell Physiology","external_id":{"pmid":["39829340"],"isi":["001436802900001"]},"corr_author":"1","day":"05","language":[{"iso":"eng"}],"scopus_import":"1","oa_version":"None","article_number":"pcaf008","year":"2025","abstract":[{"text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species.","lang":"eng"}],"date_created":"2025-03-19T09:44:19Z","department":[{"_id":"JiFr"}],"acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","citation":{"ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. 2025. doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>.","ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>, pcaf008, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>.","apa":"Tang, H., Chen, L., &#38; Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025."},"author":[{"orcid":"0000-0001-6152-6637","first_name":"Han","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","full_name":"Tang, Han","last_name":"Tang"},{"first_name":"L","last_name":"Chen","full_name":"Chen, L"},{"full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"pmid":1,"article_type":"original"},{"citation":{"apa":"Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S., … Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. <i>Plant &#38; Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcab149\">https://doi.org/10.1093/pcp/pcab149</a>","ieee":"S. Struk <i>et al.</i>, “Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density,” <i>Plant &#38; Cell Physiology</i>, vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.","ista":"Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L, Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. Plant &#38; Cell Physiology. 63(1), 104–119.","short":"S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L. Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K. Gevaert, S. Goormachtig, Plant &#38; Cell Physiology 63 (2022) 104–119.","chicago":"Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe, Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell Physiology</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/pcp/pcab149\">https://doi.org/10.1093/pcp/pcab149</a>.","ama":"Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. <i>Plant &#38; Cell Physiology</i>. 2022;63(1):104-119. doi:<a href=\"https://doi.org/10.1093/pcp/pcab149\">10.1093/pcp/pcab149</a>","mla":"Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell Physiology</i>, vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:<a href=\"https://doi.org/10.1093/pcp/pcab149\">10.1093/pcp/pcab149</a>."},"author":[{"full_name":"Struk, Sylwia","last_name":"Struk","first_name":"Sylwia"},{"last_name":"Braem","full_name":"Braem, Lukas","first_name":"Lukas"},{"first_name":"Cedrick","last_name":"Matthys","full_name":"Matthys, Cedrick"},{"first_name":"Alan","last_name":"Walton","full_name":"Walton, Alan"},{"first_name":"Nick","last_name":"Vangheluwe","full_name":"Vangheluwe, Nick"},{"first_name":"Stan","last_name":"Van Praet","full_name":"Van Praet, Stan"},{"first_name":"Lingxiang","last_name":"Jiang","full_name":"Jiang, Lingxiang"},{"first_name":"Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel","last_name":"Baster"},{"last_name":"De Cuyper","full_name":"De Cuyper, Carolien","first_name":"Carolien"},{"last_name":"Boyer","full_name":"Boyer, Francois-Didier","first_name":"Francois-Didier"},{"first_name":"Elisabeth","last_name":"Stes","full_name":"Stes, Elisabeth"},{"first_name":"Tom","full_name":"Beeckman, Tom","last_name":"Beeckman"},{"full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"},{"last_name":"Gevaert","full_name":"Gevaert, Kris","first_name":"Kris"},{"full_name":"Goormachtig, Sofie","last_name":"Goormachtig","first_name":"Sofie"}],"title":"Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density","pmid":1,"article_type":"original","abstract":[{"text":"The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac-GR24 modifies the root architecture at different levels, such as decreasing the lateral root density (LRD), while promoting root hair elongation or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis is transcriptionally activated in the root by rac-GR24 treatment, but, thus far, the molecular players involved in that response have remained unknown. To get an in-depth insight into the changes that occur after the compound is perceived by the roots, we compared the root transcriptomes of the wild type and the more axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways, with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR, reporter line analysis and mutant phenotyping indicated that the flavonol response and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5) and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS 5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding the molecular mechanisms that underlay the rac-GR24 responses in the root.","lang":"eng"}],"date_created":"2021-12-28T11:44:18Z","department":[{"_id":"JiFr"}],"acknowledgement":"The authors thank Ralf Stracke (Bielefeld University, Bielefeld, Germany) for providing the myb mutants and their colleagues Bert De Rybel for the tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique Storme for statistical help on both the RNA-seq and lateral root density, and Martine De Cock for help in preparing the manuscript.","page":"104-119","year":"2022","oa_version":"Published Version","scopus_import":"1","oa":1,"external_id":{"pmid":["34791413"],"isi":["000877899400009"]},"publication":"Plant & Cell Physiology","publication_status":"published","language":[{"iso":"eng"}],"day":"21","volume":63,"publication_identifier":{"eissn":["1471-9053"],"issn":["0032-0781"]},"keyword":["flavonols","MAX2","rac-Gr24","RNA-seq","root development","transcriptional regulation"],"isi":1,"intvolume":"        63","publisher":"Oxford University Press","article_processing_charge":"No","date_published":"2022-01-21T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","main_file_link":[{"url":"https://doi.org/10.1093/pcp/pcab149","open_access":"1"}],"status":"public","type":"journal_article","_id":"10583","date_updated":"2023-08-02T13:40:43Z","quality_controlled":"1","issue":"1","month":"01","doi":"10.1093/pcp/pcab149"},{"issue":"2","month":"02","doi":"10.1093/pcp/pcz001","date_updated":"2023-08-25T08:05:28Z","quality_controlled":"1","_id":"6104","date_published":"2019-02-01T00:00:00Z","type":"journal_article","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","status":"public","article_processing_charge":"No","isi":1,"publisher":"Oxford University Press","intvolume":"        60","publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"volume":60,"language":[{"iso":"eng"}],"day":"01","external_id":{"pmid":["30668780"],"isi":["000459634300002"]},"publication":"Plant and Cell Physiology","publication_status":"published","oa_version":"None","scopus_import":"1","year":"2019","department":[{"_id":"JiFr"}],"page":"255-273","date_created":"2019-03-17T22:59:14Z","abstract":[{"lang":"eng","text":"Abiotic stress poses constant challenges for plant survival and is a serious problem for global agricultural productivity. On a molecular level, stress conditions result in elevation of reactive oxygen species (ROS) production causing oxidative stress associated with oxidation of proteins and nucleic acids as well as impairment of membrane functions. Adaptation of root growth to ROS accumulation is facilitated through modification of auxin and cytokinin hormone homeostasis. Here, we report that in Arabidopsis root meristem, ROS-induced changes of auxin levels correspond to decreased abundance of PIN auxin efflux carriers at the plasma membrane (PM). Specifically, increase in H2O2 levels affects PIN2 endocytic recycling. We show that the PIN2 intracellular trafficking during adaptation to oxidative stress requires the function of the ADP-ribosylation factor (ARF)-guanine-nucleotide exchange factor (GEF) BEN1, an actin-associated regulator of the trafficking from the PM to early endosomes and, presumably, indirectly, trafficking to the vacuoles. We propose that H2O2 levels affect the actin dynamics thus modulating ARF-GEF-dependent trafficking of PIN2. This mechanism provides a way how root growth acclimates to stress and adapts to a changing environment."}],"pmid":1,"author":[{"first_name":"Marta","full_name":"Zwiewka, Marta","last_name":"Zwiewka"},{"full_name":"Bielach, Agnieszka","last_name":"Bielach","first_name":"Agnieszka"},{"full_name":"Tamizhselvan, Prashanth","last_name":"Tamizhselvan","first_name":"Prashanth"},{"last_name":"Madhavan","full_name":"Madhavan, Sharmila","first_name":"Sharmila"},{"first_name":"Eman Elrefaay","full_name":"Ryad, Eman Elrefaay","last_name":"Ryad"},{"full_name":"Tan, Shutang","last_name":"Tan","orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","first_name":"Mónika","last_name":"Hrtyan","full_name":"Hrtyan, Mónika"},{"full_name":"Dobrev, Petre","last_name":"Dobrev","first_name":"Petre"},{"first_name":"Radomira","full_name":"Vanková, Radomira","last_name":"Vanková"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Vanesa B.","full_name":"Tognetti, Vanesa B.","last_name":"Tognetti"}],"citation":{"mla":"Zwiewka, Marta, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” <i>Plant and Cell Physiology</i>, vol. 60, no. 2, Oxford University Press, 2019, pp. 255–73, doi:<a href=\"https://doi.org/10.1093/pcp/pcz001\">10.1093/pcp/pcz001</a>.","ista":"Zwiewka M, Bielach A, Tamizhselvan P, Madhavan S, Ryad EE, Tan S, Hrtyan M, Dobrev P, Vanková R, Friml J, Tognetti VB. 2019. Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. Plant and Cell Physiology. 60(2), 255–273.","short":"M. Zwiewka, A. Bielach, P. Tamizhselvan, S. Madhavan, E.E. Ryad, S. Tan, M. Hrtyan, P. Dobrev, R. Vanková, J. Friml, V.B. Tognetti, Plant and Cell Physiology 60 (2019) 255–273.","ama":"Zwiewka M, Bielach A, Tamizhselvan P, et al. Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. <i>Plant and Cell Physiology</i>. 2019;60(2):255-273. doi:<a href=\"https://doi.org/10.1093/pcp/pcz001\">10.1093/pcp/pcz001</a>","chicago":"Zwiewka, Marta, Agnieszka Bielach, Prashanth Tamizhselvan, Sharmila Madhavan, Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2019. <a href=\"https://doi.org/10.1093/pcp/pcz001\">https://doi.org/10.1093/pcp/pcz001</a>.","ieee":"M. Zwiewka <i>et al.</i>, “Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking,” <i>Plant and Cell Physiology</i>, vol. 60, no. 2. Oxford University Press, pp. 255–273, 2019.","apa":"Zwiewka, M., Bielach, A., Tamizhselvan, P., Madhavan, S., Ryad, E. E., Tan, S., … Tognetti, V. B. (2019). Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcz001\">https://doi.org/10.1093/pcp/pcz001</a>"},"title":"Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking"}]