---
OA_type: closed access
_id: '20079'
abstract:
- lang: eng
text: "Research question: Is LINC01638 involved in regulation of epithelial-to-mesenchymal
transition (EMT) in endometriosis?\r\nDesign: A prospective patient cohort study
was combined with functional experiments in the 12Z endometriosis epithelial cell
line to investigate the role of LINC01638 in endometriosis. Eutopic endometrial
samples were collected by curettage, and ectopic endometrial lesion samples were
collected by laparoscopic surgery from 24 control patients and 41 patients with
endometriosis. The phenotype of 12Z cells was assessed following LINC01638 knockdown
using siRNA, performing proliferation, adhesion, migration and invasion assays,
as well as assessing apoptosis and cell cycle changes with flow cytometry assays.
In order to assess the relationship between LINC01638 and histone deacetylase
class 1 enzyme (HDAC1), LINC01638 knockdown was combined with HDAC inhibition
with the specific HDAC inhibitor romidepsin.\r\nResults: LINC01638 was up-regulated
in the epithelial layer of endometriotic lesions, and LINC01638 knockdown in 12Z
cells led to reduced proliferation, adhesion, migration and invasion. The reduction
in proliferation was associated with increased p21 and p27 expression, and G1
phase arrest. Further analysis of LINC01638 control and knockdown cells revealed
that a number of transcription factors associated with EMT are down-regulated
in knockdown cells, along with the cytoskeleton regulatory gene RHOB, while HDAC1
was up-regulated. Chromatin immunoprecipitation analysis and HDAC1 inhibitory
treatment combined with LINC01638 knockdown indicated that LINC01638 regulates
RHOB expression via HDAC1-mediated promoter deacetylation. RHOB is up-regulated
in the epithelial layer of endometriotic lesions compared with eutopic endometrium,
supporting a role in the disease.\r\nConclusions: LINC01638 is an epigenetic regulator
of the pathogenesis of endometriosis, promoting proliferation and EMT of endometriotic
lesions."
acknowledgement: "The authors wish to thank all the participants and health professionals
involved in this study. In addition, the authors wish to thank technical assistants
Barbara Widmar, Matthias Witzmann-Stern and Isabella Haslinger for their work assisting
with this study; and Simon Hippenmeyer for access to bioinformatic infrastructure
and resources.\r\nOpen access funding was provided by the Medical University of
Vienna."
article_number: '104942'
article_processing_charge: No
article_type: original
author:
- first_name: Iveta
full_name: Yotova, Iveta
last_name: Yotova
- first_name: Katharina
full_name: Proestling, Katharina
last_name: Proestling
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Lisa
full_name: Rainer, Lisa
last_name: Rainer
- first_name: Leonie
full_name: Kaup, Leonie
last_name: Kaup
- first_name: Jana
full_name: Heine, Jana
last_name: Heine
- first_name: Lejla
full_name: Sandrieser, Lejla
last_name: Sandrieser
- first_name: René
full_name: Wenzl, René
last_name: Wenzl
- first_name: Quanah J.
full_name: Hudson, Quanah J.
last_name: Hudson
citation:
ama: Yotova I, Proestling K, Pauler F, et al. LINC01638 promotes epithelial-to-mesenchymal
transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. 2025;51(3). doi:10.1016/j.rbmo.2025.104942
apa: Yotova, I., Proestling, K., Pauler, F., Rainer, L., Kaup, L., Heine, J., …
Hudson, Q. J. (2025). LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. Elsevier. https://doi.org/10.1016/j.rbmo.2025.104942
chicago: Yotova, Iveta, Katharina Proestling, Florian Pauler, Lisa Rainer, Leonie
Kaup, Jana Heine, Lejla Sandrieser, René Wenzl, and Quanah J. Hudson. “LINC01638
Promotes Epithelial-to-Mesenchymal Transition in Endometriosis Epithelial Cells
by up-Regulating RHOB via HDAC1 Suppression.” Reproductive Biomedicine Online.
Elsevier, 2025. https://doi.org/10.1016/j.rbmo.2025.104942.
ieee: I. Yotova et al., “LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression,”
Reproductive Biomedicine Online, vol. 51, no. 3. Elsevier, 2025.
ista: Yotova I, Proestling K, Pauler F, Rainer L, Kaup L, Heine J, Sandrieser L,
Wenzl R, Hudson QJ. 2025. LINC01638 promotes epithelial-to-mesenchymal transition
in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression.
Reproductive Biomedicine Online. 51(3), 104942.
mla: Yotova, Iveta, et al. “LINC01638 Promotes Epithelial-to-Mesenchymal Transition
in Endometriosis Epithelial Cells by up-Regulating RHOB via HDAC1 Suppression.”
Reproductive Biomedicine Online, vol. 51, no. 3, 104942, Elsevier, 2025,
doi:10.1016/j.rbmo.2025.104942.
short: I. Yotova, K. Proestling, F. Pauler, L. Rainer, L. Kaup, J. Heine, L. Sandrieser,
R. Wenzl, Q.J. Hudson, Reproductive Biomedicine Online 51 (2025).
date_created: 2025-07-27T22:01:25Z
date_published: 2025-07-17T00:00:00Z
date_updated: 2025-09-30T14:10:46Z
day: '17'
department:
- _id: SiHi
doi: 10.1016/j.rbmo.2025.104942
external_id:
isi:
- '001549819000002'
pmid:
- '40680553'
intvolume: ' 51'
isi: 1
issue: '3'
language:
- iso: eng
month: '07'
oa_version: None
pmid: 1
publication: Reproductive Biomedicine Online
publication_identifier:
eissn:
- 1472-6491
issn:
- 1472-6483
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial
cells by up-regulating RHOB via HDAC1 suppression
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 51
year: '2025'
...