---
OA_type: closed access
_id: '4352'
abstract:
- lang: eng
  text: 'Anopheles darlingi is the primary malaria vector in Latin America, and is
    especially important in Amazonian Brazil. Historically, control efforts have been
    focused on indoor house spraying using a variety of insecticides, but since the
    mid-1990s there has been a shift to patient treatment and focal insecticide fogging.
    Anopheles darlingi was believed to have been significantly reduced in a gold-mining
    community, Peixoto de Azevedo (in Mato Grosso State), in the early 1990s by insecticide
    use during a severe malaria epidemic. In contrast, although An. darlingi was eradicated
    from some districts of the city of Belem (the capital of Para State) in 1968 to
    reduce malaria, populations around the water protection area in the eastern district
    were treated only briefly. To investigate the population structure of An. darlingi
    including evidence for a population bottleneck in Peixoto, we analyzed eight microsatellite
    loci of 256 individuals from seven locations in Brazil: three in Amapa State,
    three in Para State, and one in Mato Grosso State. Allelic diversity and mean
    expected heterozygosity were high for all populations (mean number alleles/locus
    and H(E) were 13.5 and 0.834, respectively) and did not differ significantly between
    locations. Significant heterozygote deficits were associated with linkage disequilibrium,
    most likely due to either the Wahlund effect or selection. We found no evidence
    for a population bottleneck in Peixoto, possibly because the reduction was not
    extreme enough to be detected. Overall estimates of long-term N(e) varied from
    92.4 individuals under the linkage disequilibrium model to infinity under the
    heterozygote excess model. Fixation indices and analysis of molecular variance
    demonstrated significant differentiation between locations north and south of
    the Amazon River, suggesting a degree of genetic isolation between them, attributed
    to isolation by distance.'
article_processing_charge: No
article_type: original
author:
- first_name: Jan
  full_name: Conn, Jan
  last_name: Conn
- first_name: Joseph
  full_name: Vineis, Joseph
  last_name: Vineis
- first_name: Jonathan P
  full_name: Bollback, Jonathan P
  id: 2C6FA9CC-F248-11E8-B48F-1D18A9856A87
  last_name: Bollback
  orcid: 0000-0002-4624-4612
- first_name: David
  full_name: Onyabe, David
  last_name: Onyabe
- first_name: Richard
  full_name: Wilkerson, Richard
  last_name: Wilkerson
- first_name: Marinete
  full_name: Povoa, Marinete
  last_name: Povoa
citation:
  ama: Conn J, Vineis J, Bollback JP, Onyabe D, Wilkerson R, Povoa M. Population structure
    of the malaria vector Anopheles darlingi in a malaria-endemic region of eastern
    Amazonian Brazil. <i>The American Journal of Tropical Medicine and Hygiene</i>.
    2006;74(5):798-806.
  apa: Conn, J., Vineis, J., Bollback, J. P., Onyabe, D., Wilkerson, R., &#38; Povoa,
    M. (2006). Population structure of the malaria vector Anopheles darlingi in a
    malaria-endemic region of eastern Amazonian Brazil. <i>The American Journal of
    Tropical Medicine and Hygiene</i>. American Society of Tropical Medicine and Hygiene.
  chicago: Conn, Jan, Joseph Vineis, Jonathan P Bollback, David Onyabe, Richard Wilkerson,
    and Marinete Povoa. “Population Structure of the Malaria Vector Anopheles Darlingi
    in a Malaria-Endemic Region of Eastern Amazonian Brazil.” <i>The American Journal
    of Tropical Medicine and Hygiene</i>. American Society of Tropical Medicine and
    Hygiene, 2006.
  ieee: J. Conn, J. Vineis, J. P. Bollback, D. Onyabe, R. Wilkerson, and M. Povoa,
    “Population structure of the malaria vector Anopheles darlingi in a malaria-endemic
    region of eastern Amazonian Brazil,” <i>The American Journal of Tropical Medicine
    and Hygiene</i>, vol. 74, no. 5. American Society of Tropical Medicine and Hygiene,
    pp. 798–806, 2006.
  ista: Conn J, Vineis J, Bollback JP, Onyabe D, Wilkerson R, Povoa M. 2006. Population
    structure of the malaria vector Anopheles darlingi in a malaria-endemic region
    of eastern Amazonian Brazil. The American Journal of Tropical Medicine and Hygiene.
    74(5), 798–806.
  mla: Conn, Jan, et al. “Population Structure of the Malaria Vector Anopheles Darlingi
    in a Malaria-Endemic Region of Eastern Amazonian Brazil.” <i>The American Journal
    of Tropical Medicine and Hygiene</i>, vol. 74, no. 5, American Society of Tropical
    Medicine and Hygiene, 2006, pp. 798–806.
  short: J. Conn, J. Vineis, J.P. Bollback, D. Onyabe, R. Wilkerson, M. Povoa, The
    American Journal of Tropical Medicine and Hygiene 74 (2006) 798–806.
date_created: 2018-12-11T12:08:25Z
date_published: 2006-05-01T00:00:00Z
date_updated: 2026-08-21T11:53:02Z
day: '01'
extern: '1'
external_id:
  pmid:
  - '16687683'
intvolume: '        74'
issue: '5'
language:
- iso: eng
main_file_link:
- url: http://www.ajtmh.org/content/74/5/798.full
month: '05'
oa_version: None
page: 798 - 806
pmid: 1
publication: The American Journal of Tropical Medicine and Hygiene
publication_identifier:
  eissn:
  - 1476-1645
  issn:
  - 0002-9637
publication_status: published
publisher: American Society of Tropical Medicine and Hygiene
publist_id: '1108'
status: public
title: Population structure of the malaria vector Anopheles darlingi in a malaria-endemic
  region of eastern Amazonian Brazil
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 74
year: '2006'
...