---
OA_place: repository
OA_type: green
_id: '21039'
abstract:
- lang: eng
text: Cellular plasticity, the ability of a differentiated cell to adopt another
phenotypic identity, is restricted under basal conditions, but can be elicited
upon damage. However, the molecular mechanism enabling such plasticity remains
largely unexplored. Here, we report damage-induced cellular plasticity of secretory
enteroendocrine cells (EEs) in the adult Drosophila midgut. Ionizing radiation
induces EE fate conversion and activates stress-responsive programs in EE lineages,
accompanied by the induction of the stress-inducible transcription factor Xrp1
and the cytokine gene upd3. Xrp1 and upd3 are both necessary for radiation-induced
EE plasticity. Under basal conditions, EE-specific Xrp1 overexpression triggers
ectopic expression of progenitor-specific genes, which is necessary for Xrp1 to
drive EE plasticity. Our work identifies Xrp1 as a crucial regulator that coordinates
damage-induced signaling and transcriptional reprogramming, enabling the reactivation
of cellular plasticity in differentiated cells.
acknowledgement: We thank Pierre Léopold, Tatsushi Igaki, Erik Storkebaum, Tobias
Reiff, Masayuki Miura, Xiaohang Yang, Mikio Furuse, Bloomington Drosophila Stock
Center and Developmental Studies Hybridoma Bank for providing us with fly stocks
and reagents. We are also grateful to Hiromi Yanagisawa, Satoru Kobayashi, Md Al
Amin Sheikh and Yaxuan Cui for allowing us to use their equipment, and to Allison
Bardin, Pierre Léopold and Tadashi Uemura for helpful discussions.
article_number: dev205225
article_processing_charge: No
article_type: original
author:
- first_name: Qingyin
full_name: Qian, Qingyin
last_name: Qian
- first_name: Hiroki
full_name: Nagai, Hiroki
id: 608df3e6-e2ab-11ed-8890-c9318cec7da4
last_name: Nagai
orcid: 0000-0003-1671-9434
- first_name: Yuya
full_name: Sanaki, Yuya
last_name: Sanaki
- first_name: Makoto
full_name: Hayashi, Makoto
last_name: Hayashi
- first_name: Kenichi
full_name: Kimura, Kenichi
last_name: Kimura
- first_name: Yu Ichiro
full_name: Nakajima, Yu Ichiro
last_name: Nakajima
- first_name: Ryusuke
full_name: Niwa, Ryusuke
last_name: Niwa
citation:
ama: Qian Q, NAGAI H, Sanaki Y, et al. Xrp1 drives damage-induced cellular plasticity
of enteroendocrine cells in the adult Drosophila midgut. Development. 2026;153(2).
doi:10.1242/dev.205225
apa: Qian, Q., NAGAI, H., Sanaki, Y., Hayashi, M., Kimura, K., Nakajima, Y. I.,
& Niwa, R. (2026). Xrp1 drives damage-induced cellular plasticity of enteroendocrine
cells in the adult Drosophila midgut. Development. The Company of Biologists.
https://doi.org/10.1242/dev.205225
chicago: Qian, Qingyin, HIROKI NAGAI, Yuya Sanaki, Makoto Hayashi, Kenichi Kimura,
Yu Ichiro Nakajima, and Ryusuke Niwa. “Xrp1 Drives Damage-Induced Cellular Plasticity
of Enteroendocrine Cells in the Adult Drosophila Midgut.” Development.
The Company of Biologists, 2026. https://doi.org/10.1242/dev.205225.
ieee: Q. Qian et al., “Xrp1 drives damage-induced cellular plasticity of
enteroendocrine cells in the adult Drosophila midgut,” Development, vol.
153, no. 2. The Company of Biologists, 2026.
ista: Qian Q, NAGAI H, Sanaki Y, Hayashi M, Kimura K, Nakajima YI, Niwa R. 2026.
Xrp1 drives damage-induced cellular plasticity of enteroendocrine cells in the
adult Drosophila midgut. Development. 153(2), dev205225.
mla: Qian, Qingyin, et al. “Xrp1 Drives Damage-Induced Cellular Plasticity of Enteroendocrine
Cells in the Adult Drosophila Midgut.” Development, vol. 153, no. 2, dev205225,
The Company of Biologists, 2026, doi:10.1242/dev.205225.
short: Q. Qian, H. NAGAI, Y. Sanaki, M. Hayashi, K. Kimura, Y.I. Nakajima, R. Niwa,
Development 153 (2026).
date_created: 2026-01-25T23:01:39Z
date_published: 2026-01-15T00:00:00Z
date_updated: 2026-02-12T12:41:18Z
day: '15'
department:
- _id: XiFe
doi: 10.1242/dev.205225
external_id:
pmid:
- '41392708'
intvolume: ' 153'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.07.05.662934
month: '01'
oa: 1
oa_version: Preprint
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Xrp1 drives damage-induced cellular plasticity of enteroendocrine cells in
the adult Drosophila midgut
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 153
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20048'
abstract:
- lang: eng
text: 'During embryonic development, cell behaviors need to be tightly regulated
in time and space. Yet how the temporal and spatial regulations of cell behaviors
are interconnected during embryonic development remains elusive. To address this,
we turned to zebrafish gastrulation, the process whereby dynamic cell behaviors
generate the three principal germ layers of the early embryo. Here, we show that
Hoxb cluster genes are expressed in a temporally collinear manner at the blastoderm
margin, where mesodermal and endodermal (mesendoderm) progenitor cells are specified
and ingress to form mesendoderm/hypoblast. Functional analysis shows that these
Hoxb genes regulate the timing of cell ingression: under- or overexpression of
Hoxb genes perturb the timing of mesendoderm cell ingression and, consequently,
the positioning of these cells along the forming anterior-posterior body axis
after gastrulation. Finally, we found that Hoxb genes control the timing of mesendoderm
ingression by regulating cellular bleb formation and cell surface fluctuations
in the ingressing cells. Collectively, our findings suggest that Hoxb genes interconnect
the temporal and spatial pattern of cell behaviors during zebrafish gastrulation
by controlling cell surface fluctuations.'
acknowledgement: We thank all the Heisenberg lab members for discussions and comments
on the manuscript, and the Bioimaging and Life Science facilities of ISTA for support
with microscopy and fish maintenance, respectively. This study was funded by a Japan
Society for the Promotion of Science (JSPS) Overseas Research Fellowship and a Japan
Science and Technology Agency PRESTO grant (JPMJPR214B) to Y.M. Open Access funding
provided by the Japan Science and Technology Agency. Deposited in PMC for immediate
release.
article_number: dev204261
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuuta
full_name: Moriyama, Yuuta
id: addc9b8c-67a0-11f0-b374-a2e094825470
last_name: Moriyama
orcid: 0000-0002-2853-8051
- first_name: Toshiyuki
full_name: Mitsui, Toshiyuki
last_name: Mitsui
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Moriyama Y, Mitsui T, Heisenberg C-PJ. Hoxb genes determine the timing of cell
ingression by regulating cell surface fluctuations during zebrafish gastrulation.
Development. 2025;152(12). doi:10.1242/dev.204261
apa: Moriyama, Y., Mitsui, T., & Heisenberg, C.-P. J. (2025). Hoxb genes determine
the timing of cell ingression by regulating cell surface fluctuations during zebrafish
gastrulation. Development. The Company of Biologists. https://doi.org/10.1242/dev.204261
chicago: Moriyama, Yuuta, Toshiyuki Mitsui, and Carl-Philipp J Heisenberg. “Hoxb
Genes Determine the Timing of Cell Ingression by Regulating Cell Surface Fluctuations
during Zebrafish Gastrulation.” Development. The Company of Biologists,
2025. https://doi.org/10.1242/dev.204261.
ieee: Y. Moriyama, T. Mitsui, and C.-P. J. Heisenberg, “Hoxb genes determine the
timing of cell ingression by regulating cell surface fluctuations during zebrafish
gastrulation,” Development, vol. 152, no. 12. The Company of Biologists,
2025.
ista: Moriyama Y, Mitsui T, Heisenberg C-PJ. 2025. Hoxb genes determine the timing
of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation.
Development. 152(12), dev204261.
mla: Moriyama, Yuuta, et al. “Hoxb Genes Determine the Timing of Cell Ingression
by Regulating Cell Surface Fluctuations during Zebrafish Gastrulation.” Development,
vol. 152, no. 12, dev204261, The Company of Biologists, 2025, doi:10.1242/dev.204261.
short: Y. Moriyama, T. Mitsui, C.-P.J. Heisenberg, Development 152 (2025).
corr_author: '1'
date_created: 2025-07-21T08:10:32Z
date_published: 2025-06-27T00:00:00Z
date_updated: 2025-09-30T14:07:51Z
day: '27'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1242/dev.204261
external_id:
isi:
- '001525252300001'
pmid:
- '40576478'
file:
- access_level: open_access
checksum: 808d8aa28df79d23fb661838d1fdc1be
content_type: application/pdf
creator: dernst
date_created: 2025-07-23T08:43:01Z
date_updated: 2025-07-23T08:43:01Z
file_id: '20070'
file_name: 2025_Development_Moriyama.pdf
file_size: 25935563
relation: main_file
success: 1
file_date_updated: 2025-07-23T08:43:01Z
has_accepted_license: '1'
intvolume: ' 152'
isi: 1
issue: '12'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Hoxb genes determine the timing of cell ingression by regulating cell surface
fluctuations during zebrafish gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 152
year: '2025'
...
---
_id: '15048'
abstract:
- lang: eng
text: Embryogenesis results from the coordinated activities of different signaling
pathways controlling cell fate specification and morphogenesis. In vertebrate
gastrulation, both Nodal and BMP signaling play key roles in germ layer specification
and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis
is still insufficiently understood. Here, we took a reductionist approach using
zebrafish embryonic explants to study the coordination of Nodal and BMP signaling
for embryo patterning and morphogenesis. We show that Nodal signaling triggers
explant elongation by inducing mesendodermal progenitors but also suppressing
BMP signaling activity at the site of mesendoderm induction. Consistent with this,
ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm
intercalations, key processes during explant elongation. Translating these ex
vivo observations to the intact embryo showed that, similar to explants, Nodal
signaling suppresses the effect of BMP signaling on cell intercalations in the
dorsal domain, thus allowing robust embryonic axis elongation. These findings
suggest a dual function of Nodal signaling in embryonic axis elongation by both
inducing mesendoderm and suppressing BMP effects in the dorsal portion of the
mesendoderm.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Patrick Müller for sharing the chordintt250 mutant zebrafish
line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b
plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated
the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro
and Katherine Rogers and members of the Heisenberg lab for discussions, technical
advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo
for discussions. We thank the Imaging and Optics Facility as well as the Life Science
facility at IST Austria for support with microscopy and fish maintenance.\r\nThis
work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573
to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy
of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience
and Technology Austria. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Kornelija
full_name: Pranjic-Ferscha, Kornelija
id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
last_name: Pranjic-Ferscha
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation
by Nodal-dependent restriction of BMP signaling. Development. 2024;151(4):1-18.
doi:10.1242/dev.202316
apa: Schauer, A., Pranjic-Ferscha, K., Hauschild, R., & Heisenberg, C.-P. J.
(2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling.
Development. The Company of Biologists. https://doi.org/10.1242/dev.202316
chicago: Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp
J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.”
Development. The Company of Biologists, 2024. https://doi.org/10.1242/dev.202316.
ieee: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust
axis elongation by Nodal-dependent restriction of BMP signaling,” Development,
vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.
ista: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis
elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4),
1–18.
mla: Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction
of BMP Signaling.” Development, vol. 151, no. 4, The Company of Biologists,
2024, pp. 1–18, doi:10.1242/dev.202316.
short: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development
151 (2024) 1–18.
corr_author: '1'
date_created: 2024-03-03T23:00:50Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T12:10:40Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1242/dev.202316
ec_funded: 1
external_id:
isi:
- '001170580200001'
pmid:
- '38372390'
file:
- access_level: open_access
checksum: 6961ea10012bf0d266681f9628bb8f13
content_type: application/pdf
creator: dernst
date_created: 2024-03-04T07:24:43Z
date_updated: 2024-03-04T07:24:43Z
file_id: '15050'
file_name: 2024_Development_Schauer.pdf
file_size: 14839986
relation: main_file
success: 1
file_date_updated: 2024-03-04T07:24:43Z
has_accepted_license: '1'
intvolume: ' 151'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1-18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14926'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Robust axis elongation by Nodal-dependent restriction of BMP signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 151
year: '2024'
...
---
_id: '17458'
abstract:
- lang: eng
text: Changes in gene dosage can have tremendous evolutionary potential (e.g. whole-genome
duplications), but without compensatory mechanisms, they can also lead to gene
dysregulation and pathologies. Sex chromosomes are a paradigmatic example of naturally
occurring gene dosage differences and their compensation. In species with chromosome-based
sex determination, individuals within the same population necessarily show ‘natural’
differences in gene dosage for the sex chromosomes. In this Review, we focus on
the mammalian X chromosome and discuss recent new insights into the dosage-compensation
mechanisms that evolved along with the emergence of sex chromosomes, namely X-inactivation
and X-upregulation. We also discuss the evolution of the genetic loci and molecular
players involved, as well as the regulatory diversity and potentially different
requirements for dosage compensation across mammalian species.
acknowledgement: We thank Estelle Nicolas for critical feedback on the manuscript
and Ikuhiro Okamoto for critical feedback on the figures. We apologise to authors
whose work we overlooked or did not discuss or cite due to limits in the number
of references. We thank the anonymous reviewers for pointing us to additional literature
and for their constructive feedback. Figures were prepared with BioRender.com. D.C.
is supported by a fellowship from Ligue Contre le Cancer (LNCC_TAJT25850) and R.G.
holds a tenured research position from the Centre National de la Recherche Scientifique
(France). Research in the Galupa lab is supported by a grant from the Fondation
pour la Recherche Médicale (AJE202305017142). Open Access funding provided by Fondation
pour la Recherche Médicale. Deposited in PMC for immediate release.
article_number: dev202891
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Daniela
full_name: Cecalev, Daniela
last_name: Cecalev
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
- first_name: Rafael
full_name: Galupa, Rafael
last_name: Galupa
citation:
ama: Cecalev D, Vicoso B, Galupa R. Compensation of gene dosage on the mammalian
X. Development. 2024;151(15). doi:10.1242/dev.202891
apa: Cecalev, D., Vicoso, B., & Galupa, R. (2024). Compensation of gene dosage
on the mammalian X. Development. The Company of Biologists. https://doi.org/10.1242/dev.202891
chicago: Cecalev, Daniela, Beatriz Vicoso, and Rafael Galupa. “Compensation of Gene
Dosage on the Mammalian X.” Development. The Company of Biologists, 2024.
https://doi.org/10.1242/dev.202891.
ieee: D. Cecalev, B. Vicoso, and R. Galupa, “Compensation of gene dosage on the
mammalian X,” Development, vol. 151, no. 15. The Company of Biologists,
2024.
ista: Cecalev D, Vicoso B, Galupa R. 2024. Compensation of gene dosage on the mammalian
X. Development. 151(15), dev202891.
mla: Cecalev, Daniela, et al. “Compensation of Gene Dosage on the Mammalian X.”
Development, vol. 151, no. 15, dev202891, The Company of Biologists, 2024,
doi:10.1242/dev.202891.
short: D. Cecalev, B. Vicoso, R. Galupa, Development 151 (2024).
date_created: 2024-08-25T22:01:07Z
date_published: 2024-08-14T00:00:00Z
date_updated: 2025-09-08T08:58:58Z
day: '14'
ddc:
- '599'
department:
- _id: BeVi
doi: 10.1242/dev.202891
external_id:
isi:
- '001292608800003'
pmid:
- '39140247'
file:
- access_level: open_access
checksum: 5e428bda0440d3f957c694b315a8f2a9
content_type: application/pdf
creator: cchlebak
date_created: 2024-08-28T10:32:16Z
date_updated: 2024-08-28T10:32:16Z
file_id: '17464'
file_name: 2024_Development_Cecalev.pdf
file_size: 2085135
relation: main_file
success: 1
file_date_updated: 2024-08-28T10:32:16Z
has_accepted_license: '1'
intvolume: ' 151'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Compensation of gene dosage on the mammalian X
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 151
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18621'
abstract:
- lang: eng
text: During neural development, cellular adhesion is crucial for interactions among
and between neurons and surrounding tissues. This function is mediated by conserved
cell adhesion molecules, which are tightly regulated to allow for coordinated
neuronal outgrowth. Here, we show that the proprotein convertase KPC-1 (homolog
of mammalian furin) regulates the Menorin adhesion complex during development
of PVD dendritic arbors in Caenorhabditis elegans. We found a finely regulated
antagonistic balance between PVD-expressed KPC-1 and the epidermally expressed
putative cell adhesion molecule MNR-1 (Menorin). Genetically, partial loss of
mnr-1 suppressed partial loss of kpc-1, and both loss of kpc-1 and transgenic
overexpression of mnr-1 resulted in indistinguishable phenotypes in PVD dendrites.
This balance regulated cell-surface localization of the DMA-1 leucine-rich transmembrane
receptor in PVD neurons. Lastly, kpc-1 mutants showed increased amounts of MNR-1
and decreased amounts of muscle-derived LECT-2 (Chondromodulin II), which is also
part of the Menorin adhesion complex. These observations suggest that KPC-1 in
PVD neurons directly or indirectly controls the abundance of proteins of the Menorin
adhesion complex from adjacent tissues, thereby providing negative feedback from
the dendrite to the instructive cues of surrounding tissues.
acknowledgement: "We thank members of the Bülow laboratory for comments on the manuscript
and discussions throughout the course of this work; and Ryan Peer and William Corman
for their initial help with the modifier genetic screen. We acknowledge the Genomics
Core facility and the Advanced Imaging Facility at Albert Einstein College of Medicine
for help during these studies. We are grateful to Kang Shen, David Miller and the
Caenorhabditis Genetics Center (which is funded by National Institutes of Health
Office of Research Infrastructure Programs P40OD0104400) for some of the strains
used in this study, and Lhisia Chen for the anti-SAX-7 antibody.\r\nThis work was
supported by grants from the National Institutes of Health (F31NS100370 to M.R.;
T32GM007288 and F31NS111939 to M.T.; R01NS096672, R21NS081505 and R01NS129992 to
H.E.B.; and P30HD071593 to Albert Einstein College of Medicine). N.J.R.-S. was the
recipient of a Colciencias-Fulbright Fellowship [funded by Departamento Administrativo
de Ciencia, Tecnología e Innovación (COLCIENCIAS) and Fulbright Colombia], L.T.H.T.
of a Croucher Foundation Fellowship, and H.E.B. of an Irma T. Hirschl Trust/Monique
Weill-Caulier Trust research fellowship. Open Access funding provided by Albert
Einstein College of Medicine, Yeshiva University. Deposited in PMC for immediate
release."
article_processing_charge: No
article_type: original
author:
- first_name: Nelson
full_name: Ramirez, Nelson
id: 39831956-E4FE-11E9-85DE-0DC7E5697425
last_name: Ramirez
- first_name: Helen M.
full_name: Belalcazar, Helen M.
last_name: Belalcazar
- first_name: Maisha
full_name: Rahman, Maisha
last_name: Rahman
- first_name: Meera
full_name: Trivedi, Meera
last_name: Trivedi
- first_name: Leo T. H.
full_name: Tang, Leo T. H.
last_name: Tang
- first_name: Hannes E.
full_name: Bülow, Hannes E.
last_name: Bülow
citation:
ama: Ramirez N, Belalcazar HM, Rahman M, Trivedi M, Tang LTH, Bülow HE. Convertase-dependent
regulation of membrane-tethered and secreted ligands tunes dendrite adhesion.
Development. 2023;150(18). doi:10.1242/dev.201208
apa: Ramirez, N., Belalcazar, H. M., Rahman, M., Trivedi, M., Tang, L. T. H., &
Bülow, H. E. (2023). Convertase-dependent regulation of membrane-tethered and
secreted ligands tunes dendrite adhesion. Development. The Company of Biologists.
https://doi.org/10.1242/dev.201208
chicago: Ramirez, Nelson, Helen M. Belalcazar, Maisha Rahman, Meera Trivedi, Leo
T. H. Tang, and Hannes E. Bülow. “Convertase-Dependent Regulation of Membrane-Tethered
and Secreted Ligands Tunes Dendrite Adhesion.” Development. The Company
of Biologists, 2023. https://doi.org/10.1242/dev.201208.
ieee: N. Ramirez, H. M. Belalcazar, M. Rahman, M. Trivedi, L. T. H. Tang, and H.
E. Bülow, “Convertase-dependent regulation of membrane-tethered and secreted ligands
tunes dendrite adhesion,” Development, vol. 150, no. 18. The Company of
Biologists, 2023.
ista: Ramirez N, Belalcazar HM, Rahman M, Trivedi M, Tang LTH, Bülow HE. 2023. Convertase-dependent
regulation of membrane-tethered and secreted ligands tunes dendrite adhesion.
Development. 150(18).
mla: Ramirez, Nelson, et al. “Convertase-Dependent Regulation of Membrane-Tethered
and Secreted Ligands Tunes Dendrite Adhesion.” Development, vol. 150, no.
18, The Company of Biologists, 2023, doi:10.1242/dev.201208.
short: N. Ramirez, H.M. Belalcazar, M. Rahman, M. Trivedi, L.T.H. Tang, H.E. Bülow,
Development 150 (2023).
date_created: 2024-12-04T22:02:52Z
date_published: 2023-09-18T00:00:00Z
date_updated: 2024-12-09T11:43:40Z
day: '18'
ddc:
- '570'
doi: 10.1242/dev.201208
extern: '1'
external_id:
pmid:
- '37721334'
file:
- access_level: open_access
checksum: d2158dc56db50457e6404c4afec4401c
content_type: application/pdf
creator: nramirez
date_created: 2024-12-04T22:12:04Z
date_updated: 2024-12-04T22:12:04Z
file_id: '18624'
file_name: dev201208.pdf
file_size: 9559527
relation: main_file
success: 1
file_date_updated: 2024-12-04T22:12:04Z
has_accepted_license: '1'
intvolume: ' 150'
issue: '18'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Convertase-dependent regulation of membrane-tethered and secreted ligands tunes
dendrite adhesion
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 150
year: '2023'
...
---
_id: '14774'
abstract:
- lang: eng
text: Morphogen gradients impart positional information to cells in a homogenous
tissue field. Fgf8a, a highly conserved growth factor, has been proposed to act
as a morphogen during zebrafish gastrulation. However, technical limitations have
so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation
of its morphogenic activity. Here, we monitor Fgf8a propagation in the developing
neural plate using a CRISPR/Cas9-mediated EGFP knock-in at the endogenous fgf8a
locus. By combining sensitive imaging with single-molecule fluorescence correlation
spectroscopy, we demonstrate that Fgf8a, which is produced at the embryonic margin,
propagates by diffusion through the extracellular space and forms a graded distribution
towards the animal pole. Overlaying the Fgf8a gradient curve with expression profiles
of its downstream targets determines the precise input-output relationship of
Fgf8a-mediated patterning. Manipulation of the extracellular Fgf8a levels alters
the signaling outcome, thus establishing Fgf8a as a bona fide morphogen during
zebrafish gastrulation. Furthermore, by hindering Fgf8a diffusion, we demonstrate
that extracellular diffusion of the protein from the source is crucial for it
to achieve its morphogenic potential.
acknowledgement: "We thank members of the Brand lab, as well as Justina Stark (Ivo
Sbalzarini group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden,
Germany) for project-related discussions; Darren Gilmour (University of Zurich),
Karuna Sampath (University of Warwick) and Gokul Kesavan (Vowels Lifesciences Private
Limited, Bangalore) for comments on the manuscript; personnel of the CMCB technology
platform, TU Dresden for imaging and image analysis-related support; and Maurizio
Abbate (Technical support, Arivis) for help with image analysis. We are also grateful
to Stapornwongkul and Briscoe for commenting on a preprint version of our work (Stapornwongkul
and Briscoe, 2022).\r\nThis work was supported by the Deutsche Forschungsgemeinschaft
(BR 1746/6-2, BR 1746/11-1 and BR 1746/3 to M.B.), by a Cluster of Excellence ‘Physics
of Life’ seed grant and by institutional funds from Technische Universitat Dresden
(to M.B.). Open Access funding provided by Technische Universitat Dresden. Deposited
in PMC for immediate release."
article_number: dev201559
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Rohit K
full_name: Harish, Rohit K
id: 1bae78aa-ee0e-11ec-9b76-bc42990f409d
last_name: Harish
- first_name: Mansi
full_name: Gupta, Mansi
last_name: Gupta
- first_name: Daniela
full_name: Zöller, Daniela
last_name: Zöller
- first_name: Hella
full_name: Hartmann, Hella
last_name: Hartmann
- first_name: Ali
full_name: Gheisari, Ali
last_name: Gheisari
- first_name: Anja
full_name: Machate, Anja
last_name: Machate
- first_name: Stefan
full_name: Hans, Stefan
last_name: Hans
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
citation:
ama: Harish RK, Gupta M, Zöller D, et al. Real-time monitoring of an endogenous
Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation.
Development. 2023;150(19). doi:10.1242/dev.201559
apa: Harish, R. K., Gupta, M., Zöller, D., Hartmann, H., Gheisari, A., Machate,
A., … Brand, M. (2023). Real-time monitoring of an endogenous Fgf8a gradient attests
to its role as a morphogen during zebrafish gastrulation. Development.
The Company of Biologists. https://doi.org/10.1242/dev.201559
chicago: Harish, Rohit K, Mansi Gupta, Daniela Zöller, Hella Hartmann, Ali Gheisari,
Anja Machate, Stefan Hans, and Michael Brand. “Real-Time Monitoring of an Endogenous
Fgf8a Gradient Attests to Its Role as a Morphogen during Zebrafish Gastrulation.”
Development. The Company of Biologists, 2023. https://doi.org/10.1242/dev.201559.
ieee: R. K. Harish et al., “Real-time monitoring of an endogenous Fgf8a gradient
attests to its role as a morphogen during zebrafish gastrulation,” Development,
vol. 150, no. 19. The Company of Biologists, 2023.
ista: Harish RK, Gupta M, Zöller D, Hartmann H, Gheisari A, Machate A, Hans S, Brand
M. 2023. Real-time monitoring of an endogenous Fgf8a gradient attests to its role
as a morphogen during zebrafish gastrulation. Development. 150(19), dev201559.
mla: Harish, Rohit K., et al. “Real-Time Monitoring of an Endogenous Fgf8a Gradient
Attests to Its Role as a Morphogen during Zebrafish Gastrulation.” Development,
vol. 150, no. 19, dev201559, The Company of Biologists, 2023, doi:10.1242/dev.201559.
short: R.K. Harish, M. Gupta, D. Zöller, H. Hartmann, A. Gheisari, A. Machate, S.
Hans, M. Brand, Development 150 (2023).
date_created: 2024-01-10T09:18:54Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2024-01-10T12:45:25Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.201559
external_id:
isi:
- '001097449100002'
pmid:
- '37665167'
file:
- access_level: open_access
checksum: 2d6f52dc33260a9b2352b8f28374ba5f
content_type: application/pdf
creator: dernst
date_created: 2024-01-10T12:41:13Z
date_updated: 2024-01-10T12:41:13Z
file_id: '14790'
file_name: 2023_Development_Harish.pdf
file_size: 12836306
relation: main_file
success: 1
file_date_updated: 2024-01-10T12:41:13Z
has_accepted_license: '1'
intvolume: ' 150'
isi: 1
issue: '19'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
status: public
title: Real-time monitoring of an endogenous Fgf8a gradient attests to its role as
a morphogen during zebrafish gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 150
year: '2023'
...
---
_id: '12231'
abstract:
- lang: eng
text: Ventral tail bending, which is transient but pronounced, is found in many
chordate embryos and constitutes an interesting model of how tissue interactions
control embryo shape. Here, we identify one key upstream regulator of ventral
tail bending in embryos of the ascidian Ciona. We show that during the early tailbud
stages, ventral epidermal cells exhibit a boat-shaped morphology (boat cell) with
a narrow apical surface where phosphorylated myosin light chain (pMLC) accumulates.
We further show that interfering with the function of the BMP ligand Admp led
to pMLC localizing to the basal instead of the apical side of ventral epidermal
cells and a reduced number of boat cells. Finally, we show that cutting ventral
epidermal midline cells at their apex using an ultraviolet laser relaxed ventral
tail bending. Based on these results, we propose a previously unreported function
for Admp in localizing pMLC to the apical side of ventral epidermal cells, which
causes the tail to bend ventrally by resisting antero-posterior notochord extension
at the ventral side of the tail.
acknowledgement: "iona intestinalis adults were provided by Dr Yutaka Satou (Kyoto
University) and Dr Manabu Yoshida (the University of Tokyo) with support from the
National Bio-Resource Project of AMED, Japan. We thank Dr Hidehiko Hashimoto and
Dr Yuji Mizotani for technical information about 1P-myosin antibody staining. We
thank Dr Kaoru Imai and Dr Yutaka Satou for valuable discussion about Admp and for
the DNA construct of Bmp2/4 under the Dlx.b upstream sequence. We thank Ms Maki
Kogure for constructing the FUSION360 of the intercalating epidermal cell.\r\nThis
work was supported by funding from the Japan Society for the Promotion of Science
(JP16H01451, JP21H00440). Open Access funding provided by Keio University: Keio
Gijuku Daigaku."
article_number: dev200215
article_processing_charge: No
article_type: original
author:
- first_name: Yuki S.
full_name: Kogure, Yuki S.
last_name: Kogure
- first_name: Hiromochi
full_name: Muraoka, Hiromochi
last_name: Muraoka
- first_name: Wataru C.
full_name: Koizumi, Wataru C.
last_name: Koizumi
- first_name: Raphaël
full_name: Gelin-alessi, Raphaël
last_name: Gelin-alessi
- first_name: Benoit G
full_name: Godard, Benoit G
id: 33280250-F248-11E8-B48F-1D18A9856A87
last_name: Godard
- first_name: Kotaro
full_name: Oka, Kotaro
last_name: Oka
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Kohji
full_name: Hotta, Kohji
last_name: Hotta
citation:
ama: Kogure YS, Muraoka H, Koizumi WC, et al. Admp regulates tail bending by controlling
ventral epidermal cell polarity via phosphorylated myosin localization in Ciona.
Development. 2022;149(21). doi:10.1242/dev.200215
apa: Kogure, Y. S., Muraoka, H., Koizumi, W. C., Gelin-alessi, R., Godard, B. G.,
Oka, K., … Hotta, K. (2022). Admp regulates tail bending by controlling ventral
epidermal cell polarity via phosphorylated myosin localization in Ciona. Development.
The Company of Biologists. https://doi.org/10.1242/dev.200215
chicago: Kogure, Yuki S., Hiromochi Muraoka, Wataru C. Koizumi, Raphaël Gelin-alessi,
Benoit G Godard, Kotaro Oka, Carl-Philipp J Heisenberg, and Kohji Hotta. “Admp
Regulates Tail Bending by Controlling Ventral Epidermal Cell Polarity via Phosphorylated
Myosin Localization in Ciona.” Development. The Company of Biologists,
2022. https://doi.org/10.1242/dev.200215.
ieee: Y. S. Kogure et al., “Admp regulates tail bending by controlling ventral
epidermal cell polarity via phosphorylated myosin localization in Ciona,” Development,
vol. 149, no. 21. The Company of Biologists, 2022.
ista: Kogure YS, Muraoka H, Koizumi WC, Gelin-alessi R, Godard BG, Oka K, Heisenberg
C-PJ, Hotta K. 2022. Admp regulates tail bending by controlling ventral epidermal
cell polarity via phosphorylated myosin localization in Ciona. Development. 149(21),
dev200215.
mla: Kogure, Yuki S., et al. “Admp Regulates Tail Bending by Controlling Ventral
Epidermal Cell Polarity via Phosphorylated Myosin Localization in Ciona.” Development,
vol. 149, no. 21, dev200215, The Company of Biologists, 2022, doi:10.1242/dev.200215.
short: Y.S. Kogure, H. Muraoka, W.C. Koizumi, R. Gelin-alessi, B.G. Godard, K. Oka,
C.-P.J. Heisenberg, K. Hotta, Development 149 (2022).
corr_author: '1'
date_created: 2023-01-16T09:50:12Z
date_published: 2022-11-01T00:00:00Z
date_updated: 2024-10-09T21:03:48Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1242/dev.200215
external_id:
isi:
- '000903991700002'
pmid:
- '36227591'
file:
- access_level: open_access
checksum: 871b9c58eb79b9e60752de25a46938d6
content_type: application/pdf
creator: dernst
date_created: 2023-01-27T10:36:50Z
date_updated: 2023-01-27T10:36:50Z
file_id: '12423'
file_name: 2022_Development_Kogure.pdf
file_size: 9160451
relation: main_file
success: 1
file_date_updated: 2023-01-27T10:36:50Z
has_accepted_license: '1'
intvolume: ' 149'
isi: 1
issue: '21'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Admp regulates tail bending by controlling ventral epidermal cell polarity
via phosphorylated myosin localization in Ciona
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 149
year: '2022'
...
---
_id: '12245'
abstract:
- lang: eng
text: MicroRNAs (miRs) have an important role in tuning dynamic gene expression.
However, the mechanism by which they are quantitatively controlled is unknown.
We show that the amount of mature miR-9, a key regulator of neuronal development,
increases during zebrafish neurogenesis in a sharp stepwise manner. We characterize
the spatiotemporal profile of seven distinct microRNA primary transcripts (pri-mir)-9s
that produce the same mature miR-9 and show that they are sequentially expressed
during hindbrain neurogenesis. Expression of late-onset pri-mir-9-1 is added on
to, rather than replacing, the expression of early onset pri-mir-9-4 and -9-5
in single cells. CRISPR/Cas9 mutation of the late-onset pri-mir-9-1 prevents the
developmental increase of mature miR-9, reduces late neuronal differentiation
and fails to downregulate Her6 at late stages. Mathematical modelling shows that
an adaptive network containing Her6 is insensitive to linear increases in miR-9
but responds to stepwise increases of miR-9. We suggest that a sharp stepwise
increase of mature miR-9 is created by sequential and additive temporal activation
of distinct loci. This may be a strategy to overcome adaptation and facilitate
a transition of Her6 to a new dynamic regime or steady state.
acknowledgement: "We are grateful to Dr Tom Pettini for the advice on smiFISH technique
and Dr Laure Bally-Cuif for sharing plasmids. The authors also thank the Biological
Services Facility, Bioimaging and Systems Microscopy Facilities of the University
of Manchester for technical support.\r\nThis work was supported by a Wellcome Trust
Senior Research Fellowship (090868/Z/09/Z) and a Wellcome Trust Investigator Award
(224394/Z/21/Z) to N.P. and a Medical Research Council Career Development Award
to C.S.M. (MR/V032534/1). J.B. was supported by a Wellcome Trust Four-Year PhD Studentship
in Basic Science (219992/Z/19/Z). Open Access funding provided by The University
of Manchester. Deposited in PMC for immediate release."
article_number: dev200474
article_processing_charge: No
article_type: original
author:
- first_name: Ximena
full_name: Soto, Ximena
last_name: Soto
- first_name: Joshua
full_name: Burton, Joshua
last_name: Burton
- first_name: Cerys S.
full_name: Manning, Cerys S.
last_name: Manning
- first_name: Thomas
full_name: Minchington, Thomas
id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
last_name: Minchington
- first_name: Robert
full_name: Lea, Robert
last_name: Lea
- first_name: Jessica
full_name: Lee, Jessica
last_name: Lee
- first_name: Jochen
full_name: Kursawe, Jochen
last_name: Kursawe
- first_name: Magnus
full_name: Rattray, Magnus
last_name: Rattray
- first_name: Nancy
full_name: Papalopulu, Nancy
last_name: Papalopulu
citation:
ama: Soto X, Burton J, Manning CS, et al. Sequential and additive expression of
miR-9 precursors control timing of neurogenesis. Development. 2022;149(19).
doi:10.1242/dev.200474
apa: Soto, X., Burton, J., Manning, C. S., Minchington, T., Lea, R., Lee, J., …
Papalopulu, N. (2022). Sequential and additive expression of miR-9 precursors
control timing of neurogenesis. Development. The Company of Biologists.
https://doi.org/10.1242/dev.200474
chicago: Soto, Ximena, Joshua Burton, Cerys S. Manning, Thomas Minchington, Robert
Lea, Jessica Lee, Jochen Kursawe, Magnus Rattray, and Nancy Papalopulu. “Sequential
and Additive Expression of MiR-9 Precursors Control Timing of Neurogenesis.” Development.
The Company of Biologists, 2022. https://doi.org/10.1242/dev.200474.
ieee: X. Soto et al., “Sequential and additive expression of miR-9 precursors
control timing of neurogenesis,” Development, vol. 149, no. 19. The Company
of Biologists, 2022.
ista: Soto X, Burton J, Manning CS, Minchington T, Lea R, Lee J, Kursawe J, Rattray
M, Papalopulu N. 2022. Sequential and additive expression of miR-9 precursors
control timing of neurogenesis. Development. 149(19), dev200474.
mla: Soto, Ximena, et al. “Sequential and Additive Expression of MiR-9 Precursors
Control Timing of Neurogenesis.” Development, vol. 149, no. 19, dev200474,
The Company of Biologists, 2022, doi:10.1242/dev.200474.
short: X. Soto, J. Burton, C.S. Manning, T. Minchington, R. Lea, J. Lee, J. Kursawe,
M. Rattray, N. Papalopulu, Development 149 (2022).
date_created: 2023-01-16T09:53:17Z
date_published: 2022-10-01T00:00:00Z
date_updated: 2023-08-04T09:41:08Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.200474
external_id:
isi:
- '000918161000003'
pmid:
- '36189829'
file:
- access_level: open_access
checksum: d7c29b74e9e4032308228cc704a30e88
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T08:35:44Z
date_updated: 2023-01-30T08:35:44Z
file_id: '12438'
file_name: 2022_Development_Soto.pdf
file_size: 9348839
relation: main_file
success: 1
file_date_updated: 2023-01-30T08:35:44Z
has_accepted_license: '1'
intvolume: ' 149'
isi: 1
issue: '19'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
link:
- relation: software
url: ' https://github.com/burtonjosh/StepwiseMir9'
scopus_import: '1'
status: public
title: Sequential and additive expression of miR-9 precursors control timing of neurogenesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 149
year: '2022'
...
---
_id: '15262'
abstract:
- lang: eng
text: The Hunchback (Hb) transcription factor is crucial for anterior-posterior
patterning of the Drosophila embryo. The maternal hb mRNA acts as a paradigm for
translational regulation due to its repression in the posterior of the embryo.
However, little is known about the translatability of zygotically transcribed
hb mRNAs. Here, we adapt the SunTag system, developed for imaging translation
at single-mRNA resolution in tissue culture cells, to the Drosophila embryo to
study the translation dynamics of zygotic hb mRNAs. Using single-molecule imaging
in fixed and live embryos, we provide evidence for translational repression of
zygotic SunTag-hb mRNAs. Whereas the proportion of SunTag-hb mRNAs translated
is initially uniform, translation declines from the anterior over time until it
becomes restricted to a posterior band in the expression domain. We discuss how
regulated hb mRNA translation may help establish the sharp Hb expression boundary,
which is a model for precision and noise during developmental patterning. Overall,
our data show how use of the SunTag method on fixed and live embryos is a powerful
combination for elucidating spatiotemporal regulation of mRNA translation in Drosophila.
article_number: dev196121.
article_processing_charge: No
article_type: original
author:
- first_name: Daisy J.
full_name: Vinter, Daisy J.
last_name: Vinter
- first_name: Caroline
full_name: Hoppe, Caroline
last_name: Hoppe
- first_name: Thomas
full_name: Minchington, Thomas
id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
last_name: Minchington
- first_name: Catherine
full_name: Sutcliffe, Catherine
last_name: Sutcliffe
- first_name: Hilary L.
full_name: Ashe, Hilary L.
last_name: Ashe
citation:
ama: Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. Dynamics of hunchback
translation in real-time and at single-mRNA resolution in the Drosophila embryo.
Development. 2021;148(18). doi:10.1242/dev.196121
apa: Vinter, D. J., Hoppe, C., Minchington, T., Sutcliffe, C., & Ashe, H. L.
(2021). Dynamics of hunchback translation in real-time and at single-mRNA resolution
in the Drosophila embryo. Development. The Company of Biologists. https://doi.org/10.1242/dev.196121
chicago: Vinter, Daisy J., Caroline Hoppe, Thomas Minchington, Catherine Sutcliffe,
and Hilary L. Ashe. “Dynamics of Hunchback Translation in Real-Time and at Single-MRNA
Resolution in the Drosophila Embryo.” Development. The Company of Biologists,
2021. https://doi.org/10.1242/dev.196121.
ieee: D. J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, and H. L. Ashe, “Dynamics
of hunchback translation in real-time and at single-mRNA resolution in the Drosophila
embryo,” Development, vol. 148, no. 18. The Company of Biologists, 2021.
ista: Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. 2021. Dynamics of
hunchback translation in real-time and at single-mRNA resolution in the Drosophila
embryo. Development. 148(18), dev196121.
mla: Vinter, Daisy J., et al. “Dynamics of Hunchback Translation in Real-Time and
at Single-MRNA Resolution in the Drosophila Embryo.” Development, vol.
148, no. 18, dev196121., The Company of Biologists, 2021, doi:10.1242/dev.196121.
short: D.J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, H.L. Ashe, Development
148 (2021).
date_created: 2024-04-03T07:26:41Z
date_published: 2021-09-01T00:00:00Z
date_updated: 2024-04-03T14:00:33Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.196121
external_id:
pmid:
- '33722899 '
file:
- access_level: open_access
checksum: 6d0533fe9c712448b3f9feb15e05ec4b
content_type: application/pdf
creator: dernst
date_created: 2024-04-03T13:58:51Z
date_updated: 2024-04-03T13:58:51Z
file_id: '15290'
file_name: 2021_CompanyBiologists_Vinter.pdf
file_size: 16258500
relation: main_file
success: 1
file_date_updated: 2024-04-03T13:58:51Z
has_accepted_license: '1'
intvolume: ' 148'
issue: '18'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dynamics of hunchback translation in real-time and at single-mRNA resolution
in the Drosophila embryo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 148
year: '2021'
...
---
_id: '9226'
abstract:
- lang: eng
text: 'Half a century after Lewis Wolpert''s seminal conceptual advance on how cellular
fates distribute in space, we provide a brief historical perspective on how the
concept of positional information emerged and influenced the field of developmental
biology and beyond. We focus on a modern interpretation of this concept in terms
of information theory, largely centered on its application to cell specification
in the early Drosophila embryo. We argue that a true physical variable (position)
is encoded in local concentrations of patterning molecules, that this mapping
is stochastic, and that the processes by which positions and corresponding cell
fates are determined based on these concentrations need to take such stochasticity
into account. With this approach, we shift the focus from biological mechanisms,
molecules, genes and pathways to quantitative systems-level questions: where does
positional information reside, how it is transformed and accessed during development,
and what fundamental limits it is subject to?'
acknowledgement: This work was supported in part by the National Science Foundation,
through the Center for the Physics of Biological Function (PHY-1734030), by the
National Institutes of Health (R01GM097275) and by the Fonds zur Förderung der wissenschaftlichen
Forschung (FWF P28844). Deposited in PMC for release after 12 months.
article_number: dev176065
article_processing_charge: No
article_type: original
author:
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Thomas
full_name: Gregor, Thomas
last_name: Gregor
citation:
ama: Tkačik G, Gregor T. The many bits of positional information. Development.
2021;148(2). doi:10.1242/dev.176065
apa: Tkačik, G., & Gregor, T. (2021). The many bits of positional information.
Development. The Company of Biologists. https://doi.org/10.1242/dev.176065
chicago: Tkačik, Gašper, and Thomas Gregor. “The Many Bits of Positional Information.”
Development. The Company of Biologists, 2021. https://doi.org/10.1242/dev.176065.
ieee: G. Tkačik and T. Gregor, “The many bits of positional information,” Development,
vol. 148, no. 2. The Company of Biologists, 2021.
ista: Tkačik G, Gregor T. 2021. The many bits of positional information. Development.
148(2), dev176065.
mla: Tkačik, Gašper, and Thomas Gregor. “The Many Bits of Positional Information.”
Development, vol. 148, no. 2, dev176065, The Company of Biologists, 2021,
doi:10.1242/dev.176065.
short: G. Tkačik, T. Gregor, Development 148 (2021).
date_created: 2021-03-07T23:01:25Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2025-04-14T09:28:43Z
day: '01'
department:
- _id: GaTk
doi: 10.1242/dev.176065
external_id:
isi:
- '000613906000007'
pmid:
- '33526425'
intvolume: ' 148'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.176065
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 254E9036-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P28844-B27
name: Biophysics of information processing in gene regulation
publication: Development
publication_identifier:
eissn:
- 1477-9129
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: The many bits of positional information
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 148
year: '2021'
...
---
_id: '7165'
abstract:
- lang: eng
text: Cell division, movement and differentiation contribute to pattern formation
in developing tissues. This is the case in the vertebrate neural tube, in which
neurons differentiate in a characteristic pattern from a highly dynamic proliferating
pseudostratified epithelium. To investigate how progenitor proliferation and differentiation
affect cell arrangement and growth of the neural tube, we used experimental measurements
to develop a mechanical model of the apical surface of the neuroepithelium that
incorporates the effect of interkinetic nuclear movement and spatially varying
rates of neuronal differentiation. Simulations predict that tissue growth and
the shape of lineage-related clones of cells differ with the rate of differentiation.
Growth is isotropic in regions of high differentiation, but dorsoventrally biased
in regions of low differentiation. This is consistent with experimental observations.
The absence of directional signalling in the simulations indicates that global
mechanical constraints are sufficient to explain the observed differences in anisotropy.
This provides insight into how the tissue growth rate affects cell dynamics and
growth anisotropy and opens up possibilities to study the coupling between mechanics,
pattern formation and growth in the neural tube.
article_number: dev176297
article_processing_charge: No
article_type: original
author:
- first_name: Pilar
full_name: Guerrero, Pilar
last_name: Guerrero
- first_name: Ruben
full_name: Perez-Carrasco, Ruben
last_name: Perez-Carrasco
- first_name: Marcin P
full_name: Zagórski, Marcin P
id: 343DA0DC-F248-11E8-B48F-1D18A9856A87
last_name: Zagórski
orcid: 0000-0001-7896-7762
- first_name: David
full_name: Page, David
last_name: Page
- first_name: Anna
full_name: Kicheva, Anna
id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
last_name: Kicheva
orcid: 0000-0003-4509-4998
- first_name: James
full_name: Briscoe, James
last_name: Briscoe
- first_name: Karen M.
full_name: Page, Karen M.
last_name: Page
citation:
ama: Guerrero P, Perez-Carrasco R, Zagórski MP, et al. Neuronal differentiation
influences progenitor arrangement in the vertebrate neuroepithelium. Development.
2019;146(23). doi:10.1242/dev.176297
apa: Guerrero, P., Perez-Carrasco, R., Zagórski, M. P., Page, D., Kicheva, A., Briscoe,
J., & Page, K. M. (2019). Neuronal differentiation influences progenitor arrangement
in the vertebrate neuroepithelium. Development. The Company of Biologists.
https://doi.org/10.1242/dev.176297
chicago: Guerrero, Pilar, Ruben Perez-Carrasco, Marcin P Zagórski, David Page, Anna
Kicheva, James Briscoe, and Karen M. Page. “Neuronal Differentiation Influences
Progenitor Arrangement in the Vertebrate Neuroepithelium.” Development.
The Company of Biologists, 2019. https://doi.org/10.1242/dev.176297.
ieee: P. Guerrero et al., “Neuronal differentiation influences progenitor
arrangement in the vertebrate neuroepithelium,” Development, vol. 146,
no. 23. The Company of Biologists, 2019.
ista: Guerrero P, Perez-Carrasco R, Zagórski MP, Page D, Kicheva A, Briscoe J, Page
KM. 2019. Neuronal differentiation influences progenitor arrangement in the vertebrate
neuroepithelium. Development. 146(23), dev176297.
mla: Guerrero, Pilar, et al. “Neuronal Differentiation Influences Progenitor Arrangement
in the Vertebrate Neuroepithelium.” Development, vol. 146, no. 23, dev176297,
The Company of Biologists, 2019, doi:10.1242/dev.176297.
short: P. Guerrero, R. Perez-Carrasco, M.P. Zagórski, D. Page, A. Kicheva, J. Briscoe,
K.M. Page, Development 146 (2019).
corr_author: '1'
date_created: 2019-12-10T14:39:50Z
date_published: 2019-12-04T00:00:00Z
date_updated: 2025-04-14T07:27:30Z
day: '04'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.176297
ec_funded: 1
external_id:
isi:
- '000507575700004'
pmid:
- '31784457'
file:
- access_level: open_access
checksum: b6533c37dc8fbd803ffeca216e0a8b8a
content_type: application/pdf
creator: dernst
date_created: 2019-12-13T07:34:06Z
date_updated: 2020-07-14T12:47:50Z
file_id: '7177'
file_name: 2019_Development_Guerrero.pdf
file_size: 7797881
relation: main_file
file_date_updated: 2020-07-14T12:47:50Z
has_accepted_license: '1'
intvolume: ' 146'
isi: 1
issue: '23'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: B6FC0238-B512-11E9-945C-1524E6697425
call_identifier: H2020
grant_number: '680037'
name: Coordination of Patterning And Growth In the Spinal Cord
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Neuronal differentiation influences progenitor arrangement in the vertebrate
neuroepithelium
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 146
year: '2019'
...
---
_id: '7404'
abstract:
- lang: eng
text: The formation of neuronal dendrite branches is fundamental for the wiring
and function of the nervous system. Indeed, dendrite branching enhances the coverage
of the neuron's receptive field and modulates the initial processing of incoming
stimuli. Complex dendrite patterns are achieved in vivo through a dynamic process
of de novo branch formation, branch extension and retraction. The first step towards
branch formation is the generation of a dynamic filopodium-like branchlet. The
mechanisms underlying the initiation of dendrite branchlets are therefore crucial
to the shaping of dendrites. Through in vivo time-lapse imaging of the subcellular
localization of actin during the process of branching of Drosophila larva sensory
neurons, combined with genetic analysis and electron tomography, we have identified
the Actin-related protein (Arp) 2/3 complex as the major actin nucleator involved
in the initiation of dendrite branchlet formation, under the control of the activator
WAVE and of the small GTPase Rac1. Transient recruitment of an Arp2/3 component
marks the site of branchlet initiation in vivo. These data position the activation
of Arp2/3 as an early hub for the initiation of branchlet formation.
article_number: dev171397
article_processing_charge: No
article_type: original
author:
- first_name: Tomke
full_name: Stürner, Tomke
last_name: Stürner
- first_name: Anastasia
full_name: Tatarnikova, Anastasia
last_name: Tatarnikova
- first_name: Jan
full_name: Müller, Jan
id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
last_name: Müller
- first_name: Barbara
full_name: Schaffran, Barbara
last_name: Schaffran
- first_name: Hermann
full_name: Cuntz, Hermann
last_name: Cuntz
- first_name: Yun
full_name: Zhang, Yun
last_name: Zhang
- first_name: Maria
full_name: Nemethova, Maria
id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
last_name: Nemethova
- first_name: Sven
full_name: Bogdan, Sven
last_name: Bogdan
- first_name: Vic
full_name: Small, Vic
last_name: Small
- first_name: Gaia
full_name: Tavosanis, Gaia
last_name: Tavosanis
citation:
ama: Stürner T, Tatarnikova A, Müller J, et al. Transient localization of the Arp2/3
complex initiates neuronal dendrite branching in vivo. Development. 2019;146(7).
doi:10.1242/dev.171397
apa: Stürner, T., Tatarnikova, A., Müller, J., Schaffran, B., Cuntz, H., Zhang,
Y., … Tavosanis, G. (2019). Transient localization of the Arp2/3 complex initiates
neuronal dendrite branching in vivo. Development. The Company of Biologists.
https://doi.org/10.1242/dev.171397
chicago: Stürner, Tomke, Anastasia Tatarnikova, Jan Müller, Barbara Schaffran, Hermann
Cuntz, Yun Zhang, Maria Nemethova, Sven Bogdan, Vic Small, and Gaia Tavosanis.
“Transient Localization of the Arp2/3 Complex Initiates Neuronal Dendrite Branching
in Vivo.” Development. The Company of Biologists, 2019. https://doi.org/10.1242/dev.171397.
ieee: T. Stürner et al., “Transient localization of the Arp2/3 complex initiates
neuronal dendrite branching in vivo,” Development, vol. 146, no. 7. The
Company of Biologists, 2019.
ista: Stürner T, Tatarnikova A, Müller J, Schaffran B, Cuntz H, Zhang Y, Nemethova
M, Bogdan S, Small V, Tavosanis G. 2019. Transient localization of the Arp2/3
complex initiates neuronal dendrite branching in vivo. Development. 146(7), dev171397.
mla: Stürner, Tomke, et al. “Transient Localization of the Arp2/3 Complex Initiates
Neuronal Dendrite Branching in Vivo.” Development, vol. 146, no. 7, dev171397,
The Company of Biologists, 2019, doi:10.1242/dev.171397.
short: T. Stürner, A. Tatarnikova, J. Müller, B. Schaffran, H. Cuntz, Y. Zhang,
M. Nemethova, S. Bogdan, V. Small, G. Tavosanis, Development 146 (2019).
date_created: 2020-01-29T16:27:10Z
date_published: 2019-04-04T00:00:00Z
date_updated: 2023-09-07T14:47:00Z
day: '04'
department:
- _id: MiSi
doi: 10.1242/dev.171397
external_id:
isi:
- '000464583200006'
pmid:
- '30910826'
intvolume: ' 146'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.171397
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transient localization of the Arp2/3 complex initiates neuronal dendrite branching
in vivo
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 146
year: '2019'
...
---
_id: '6897'
abstract:
- lang: eng
text: The apical hook is a transiently formed structure that plays a protective
role when the germinating seedling penetrates through the soil towards the surface.
Crucial for proper bending is the local auxin maxima, which defines the concave
(inner) side of the hook curvature. As no sign of asymmetric auxin distribution
has been reported in embryonic hypocotyls prior to hook formation, the question
of how auxin asymmetry is established in the early phases of seedling germination
remains largely unanswered. Here, we analyzed the auxin distribution and expression
of PIN auxin efflux carriers from early phases of germination, and show that bending
of the root in response to gravity is the crucial initial cue that governs the
hypocotyl bending required for apical hook formation. Importantly, polar auxin
transport machinery is established gradually after germination starts as a result
of tight root-hypocotyl interaction and a proper balance between abscisic acid
and gibberellins.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Jiri Friml and Phillip Brewer for inspiring discussion
and for help in preparing the manuscript. This research was supported by the Scientific
Service Units (SSU) of IST-Austria through resources provided by the Bioimaging
Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by
grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development
Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738)."
article_number: dev175919
article_processing_charge: No
article_type: original
author:
- first_name: Qiang
full_name: Zhu, Qiang
id: 40A4B9E6-F248-11E8-B48F-1D18A9856A87
last_name: Zhu
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Petra
full_name: Žádníková, Petra
last_name: Žádníková
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity
response module guides differential growth determining both root bending and apical
hook formation in Arabidopsis. Development. 2019;146(17). doi:10.1242/dev.175919
apa: Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., & Benková,
E. (2019). Root gravity response module guides differential growth determining
both root bending and apical hook formation in Arabidopsis. Development.
The Company of Biologists. https://doi.org/10.1242/dev.175919
chicago: Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad,
and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining
Both Root Bending and Apical Hook Formation in Arabidopsis.” Development.
The Company of Biologists, 2019. https://doi.org/10.1242/dev.175919.
ieee: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková,
“Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis,” Development, vol. 146,
no. 17. The Company of Biologists, 2019.
ista: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root
gravity response module guides differential growth determining both root bending
and apical hook formation in Arabidopsis. Development. 146(17), dev175919.
mla: Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth
Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” Development,
vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:10.1242/dev.175919.
short: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development
146 (2019).
date_created: 2019-09-22T22:00:36Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2026-04-03T09:45:02Z
day: '12'
department:
- _id: EvBe
doi: 10.1242/dev.175919
ec_funded: 1
external_id:
isi:
- '000486297400011'
pmid:
- '31391194'
intvolume: ' 146'
isi: 1
issue: '17'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.175919
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Development
publication_identifier:
eissn:
- 1477-9129
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 146
year: '2019'
...
---
_id: '9524'
abstract:
- lang: eng
text: Cytosine methylation is the most common covalent modification of DNA in eukaryotes.
DNA methylation has an important role in many aspects of biology, including development
and disease. Methylation can be detected using bisulfite conversion, methylation-sensitive
restriction enzymes, methyl-binding proteins and anti-methylcytosine antibodies.
Combining these techniques with DNA microarrays and high-throughput sequencing
has made the mapping of DNA methylation feasible on a genome-wide scale. Here
we discuss recent developments and future directions for identifying and mapping
methylation, in an effort to help colleagues to identify the approaches that best
serve their research interests.
article_processing_charge: No
article_type: review
author:
- first_name: Daniel
full_name: Zilberman, Daniel
id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
last_name: Zilberman
orcid: 0000-0002-0123-8649
- first_name: Steven
full_name: Henikoff, Steven
last_name: Henikoff
citation:
ama: Zilberman D, Henikoff S. Genome-wide analysis of DNA methylation patterns.
Development. 2007;134(22):3959-3965. doi:10.1242/dev.001131
apa: Zilberman, D., & Henikoff, S. (2007). Genome-wide analysis of DNA methylation
patterns. Development. The Company of Biologists. https://doi.org/10.1242/dev.001131
chicago: Zilberman, Daniel, and Steven Henikoff. “Genome-Wide Analysis of DNA Methylation
Patterns.” Development. The Company of Biologists, 2007. https://doi.org/10.1242/dev.001131.
ieee: D. Zilberman and S. Henikoff, “Genome-wide analysis of DNA methylation patterns,”
Development, vol. 134, no. 22. The Company of Biologists, pp. 3959–3965,
2007.
ista: Zilberman D, Henikoff S. 2007. Genome-wide analysis of DNA methylation patterns.
Development. 134(22), 3959–3965.
mla: Zilberman, Daniel, and Steven Henikoff. “Genome-Wide Analysis of DNA Methylation
Patterns.” Development, vol. 134, no. 22, The Company of Biologists, 2007,
pp. 3959–65, doi:10.1242/dev.001131.
short: D. Zilberman, S. Henikoff, Development 134 (2007) 3959–3965.
date_created: 2021-06-08T06:29:50Z
date_published: 2007-11-15T00:00:00Z
date_updated: 2021-12-14T08:57:58Z
day: '15'
department:
- _id: DaZi
doi: 10.1242/dev.001131
extern: '1'
external_id:
pmid:
- '17928417'
intvolume: ' 134'
issue: '22'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.001131
month: '11'
oa: 1
oa_version: Published Version
page: 3959-3965
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genome-wide analysis of DNA methylation patterns
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 134
year: '2007'
...