---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21860'
abstract:
- lang: eng
text: Glutamate excitotoxicity is a cell death mechanism triggered by accumulation
of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex
(αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point
controlling glutamate formation and its consumption as a fuel. Hence, modulation
of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic
effects. To address this hypothesis, hippocampal neurons in primary co-culture
with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through
N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures
with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion
of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover,
NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity
and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA
receptors involved mGluR1 activation and was accompanied by enhanced neuronal
discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses
evoked by glutamate application. These results suggest that mGluR1-mediated potentiation
of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might
exacerbate glutamate excitotoxicity.
acknowledgement: The technical assistance by Tanja Wagner and Elena Lilliu is gratefully
acknowledged. This research was funded in whole or in part by the Austrian Science
Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access
funding provided by Medical University of Vienna and the Austrian Science Fund (FWF).
Deposited in PMC for immediate release.
article_number: jcs264420
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Vanessa
full_name: Goeschl, Vanessa
last_name: Goeschl
- first_name: Matej
full_name: Hotka, Matej
last_name: Hotka
- first_name: Bernhard
full_name: Hochreiter, Bernhard
id: e6cab3de-17f6-11ed-9210-c1e42e045e9d
last_name: Hochreiter
- first_name: Karlheinz
full_name: Hilber, Karlheinz
last_name: Hilber
- first_name: Stefan
full_name: Boehm, Stefan
last_name: Boehm
- first_name: Andrey V.
full_name: Kozlov, Andrey V.
last_name: Kozlov
- first_name: Helmut
full_name: Kubista, Helmut
last_name: Kubista
citation:
ama: Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex
activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA
receptors in primary cultures. Journal of Cell Science. 2026;139(8). doi:10.1242/jcs.264420
apa: Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V.,
& Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates
glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary
cultures. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.264420
chicago: Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan
Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex
Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA
Receptors in Primary Cultures.” Journal of Cell Science. The Company of
Biologists, 2026. https://doi.org/10.1242/jcs.264420.
ieee: V. Goeschl et al., “α-ketoglutarate dehydrogenase complex activity
modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors
in primary cultures,” Journal of Cell Science, vol. 139, no. 8. The Company
of Biologists, 2026.
ista: Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H.
2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures. Journal of
Cell Science. 139(8), jcs264420.
mla: Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates
Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary
Cultures.” Journal of Cell Science, vol. 139, no. 8, jcs264420, The Company
of Biologists, 2026, doi:10.1242/jcs.264420.
short: V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H.
Kubista, Journal of Cell Science 139 (2026).
date_created: 2026-05-11T10:52:27Z
date_published: 2026-04-27T00:00:00Z
date_updated: 2026-05-12T06:40:18Z
day: '27'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1242/jcs.264420
external_id:
pmid:
- '41834724'
file:
- access_level: open_access
checksum: 8db35c97588c2f6ef88c7e8d5924cf8c
content_type: application/pdf
creator: dernst
date_created: 2026-05-12T06:27:54Z
date_updated: 2026-05-12T06:27:54Z
file_id: '21861'
file_name: 2026_JourCellScience_Goeschl.pdf
file_size: 1957057
relation: main_file
success: 1
file_date_updated: 2026-05-12T06:27:54Z
has_accepted_license: '1'
intvolume: ' 139'
issue: '8'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity
via metabotropic regulation of NMDA receptors in primary cultures
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 139
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20188'
abstract:
- lang: eng
text: Collective cell migration is coordinated by the front-to-rear intercellular
propagation of EGFR-Ras-ERK pathway activation. However, the molecular mechanisms
integrating front-to-rear information into this intercellular signaling cascade,
particularly the determinants of cellular front-side specification, remain elusive.
We visualized the activity of EGFR, Ras, Rac1 and Rab5A (hereafter Rab5) by using
FRET biosensors and chemogenetic tools. Whereas EGFR activation was uniformly
observed within cells, Ras activation was biased to the front side within cells.
The polarized Ras activation depended on Merlin and Rac1, which also showed front-biased
activation. Furthermore, Rab5, a crucial regulator of cell migration, demonstrated
similar front-biased activation and was found to function downstream of Ras while
being necessary for Rac1 activation. Thus, the positive feedback loop consisting
of Ras, Rab5 and Rac1 is activated primarily at the front of collectively migrating
cells. These findings offer new spatio-temporal insight into processing front–rear
information during collective cell migration.
acknowledgement: We are grateful to the members of the Matsuda Laboratory for their
helpful input, to K. Hirano, T. Uesugi and K. Takakura, who provided technical assistance,
and to the Medical Research Support Center of Kyoto University for DNA sequence
analysis. This work was supported by the Kyoto University Live Imaging Center. Financial
support was provided by Japan Society for the Promotion of Science (JSPS) KAKENHI
grants (21H05226 to K.T., 19H00993 and 20H05898 to M.M.), a Japan Science and Technology
Agency (JST) CREST grant (JPMJCR1654 to M.M.), and a JST Moonshot Research and Development
Program grant (JPMJPS2022 to M.M.). Open Access funding provided by Tokushima University.
Deposited in PMC for immediate release.
article_number: '263779'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuya
full_name: Jikko, Yuya
last_name: Jikko
- first_name: Eriko
full_name: Deguchi, Eriko
last_name: Deguchi
- first_name: Kimiya
full_name: Matsuda, Kimiya
last_name: Matsuda
- first_name: Naoya
full_name: Hino, Naoya
id: 5299a9ce-7679-11eb-a7bc-d1e62b936307
last_name: Hino
- first_name: Shinya
full_name: Tsukiji, Shinya
last_name: Tsukiji
- first_name: Michiyuki
full_name: Matsuda, Michiyuki
last_name: Matsuda
- first_name: Kenta
full_name: Terai, Kenta
last_name: Terai
citation:
ama: Jikko Y, Deguchi E, Matsuda K, et al. Front-biased activation of the Ras-Rab5-Rac1
loop coordinates collective cell migration. Journal of Cell Science. 2025;138(15).
doi:10.1242/jcs.263779
apa: Jikko, Y., Deguchi, E., Matsuda, K., Hino, N., Tsukiji, S., Matsuda, M., &
Terai, K. (2025). Front-biased activation of the Ras-Rab5-Rac1 loop coordinates
collective cell migration. Journal of Cell Science. The Company of Biologists.
https://doi.org/10.1242/jcs.263779
chicago: Jikko, Yuya, Eriko Deguchi, Kimiya Matsuda, Naoya Hino, Shinya Tsukiji,
Michiyuki Matsuda, and Kenta Terai. “Front-Biased Activation of the Ras-Rab5-Rac1
Loop Coordinates Collective Cell Migration.” Journal of Cell Science. The
Company of Biologists, 2025. https://doi.org/10.1242/jcs.263779.
ieee: Y. Jikko et al., “Front-biased activation of the Ras-Rab5-Rac1 loop
coordinates collective cell migration,” Journal of Cell Science, vol. 138,
no. 15. The Company of Biologists, 2025.
ista: Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K. 2025.
Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell
migration. Journal of Cell Science. 138(15), 263779.
mla: Jikko, Yuya, et al. “Front-Biased Activation of the Ras-Rab5-Rac1 Loop Coordinates
Collective Cell Migration.” Journal of Cell Science, vol. 138, no. 15,
263779, The Company of Biologists, 2025, doi:10.1242/jcs.263779.
short: Y. Jikko, E. Deguchi, K. Matsuda, N. Hino, S. Tsukiji, M. Matsuda, K. Terai,
Journal of Cell Science 138 (2025).
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2025-11-27T14:12:24Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1242/jcs.263779
external_id:
isi:
- '001567723900009'
pmid:
- '40667649'
file:
- access_level: open_access
checksum: 29f42619dab5ce251a20c769ed4581c0
content_type: application/pdf
creator: dernst
date_created: 2025-09-01T10:02:24Z
date_updated: 2025-09-01T10:02:24Z
file_id: '20262'
file_name: 2025_JourCellScience_Jikko.pdf
file_size: 12393297
relation: main_file
success: 1
file_date_updated: 2025-09-01T10:02:24Z
has_accepted_license: '1'
intvolume: ' 138'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- ' 0021-9533'
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell
migration
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 138
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '15330'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth
and development by controlling plasma membrane protein composition and cargo uptake.
CME relies on the precise recruitment of regulators for vesicle maturation and
release. Homologues of components of mammalian vesicle scission are strong candidates
to be part of the scission machinery in plants, but the precise roles of these
proteins in this process are not fully understood. Here, we characterised the
roles of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein
2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin,
in the CME by combining high-resolution imaging of endocytic events in vivo and
characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive
similarly late during CME and physically interact, genetic analysis of the sh3p123
triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants
suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis.
These observations imply that despite the presence of many well-conserved endocytic
components, plants have acquired a distinct mechanism for CME.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
acknowledgement: "Nataliia Gnyliukh was partially funded by the European Union’s Horizon
2020 research and\r\ninnovation program (2018-2020) under the Marie Sklodowska-Curie
Grant (agreement no.\r\n665385). Taif University Researchers Supporting Project:
TURSP-HC2022/02. and Austrian\r\nScience Fund (FWF): I 6123-B.We thank Prof. Eileen
Lafer and Liping Wang for their suggestions regarding the optimisation of protein
expression and purification. We thank Prof. Sebastian Y. Bednarek for the useful
comments and constructive criticism of the project. We thank Maciek Adamowski for
providing genetic material. This research was supported by the Scientific Service
Units (SSU) of IST-Austria through resources provided by the Electron microscopy
(EMF), Lab Support Facility (LSF) (particularly Dorota Jaworska) and the Bioimaging
Facility (BIF)."
article_number: jcs.261720
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: MK
full_name: Nagel, MK
last_name: Nagel
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: David
full_name: Babic, David
id: db566d23-f6e0-11ea-865d-e6f270e968e7
last_name: Babic
- first_name: Annamaria
full_name: Hlavata, Annamaria
id: 36062FEC-F248-11E8-B48F-1D18A9856A87
last_name: Hlavata
- first_name: SS
full_name: Alotaibi, SS
last_name: Alotaibi
- first_name: E
full_name: Isono, E
last_name: Isono
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Gnyliukh N, Johnson AJ, Nagel M, et al. Role of dynamin-related proteins 2
and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. Journal
of Cell Science. 2024;137(8). doi:10.1242/jcs.261720
apa: Gnyliukh, N., Johnson, A. J., Nagel, M., Monzer, A., Babic, D., Hlavata, A.,
… Friml, J. (2024). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated
endocytosis in Arabidopsis thaliana. Journal of Cell Science. The Company
of Biologists. https://doi.org/10.1242/jcs.261720
chicago: Gnyliukh, Nataliia, Alexander J Johnson, MK Nagel, Aline Monzer, David
Babic, Annamaria Hlavata, SS Alotaibi, E Isono, Martin Loose, and Jiří Friml.
“Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis
in Arabidopsis Thaliana.” Journal of Cell Science. The Company of Biologists,
2024. https://doi.org/10.1242/jcs.261720.
ieee: N. Gnyliukh et al., “Role of dynamin-related proteins 2 and SH3P2 in
clathrin-mediated endocytosis in Arabidopsis thaliana,” Journal of Cell Science,
vol. 137, no. 8. The Company of Biologists, 2024.
ista: Gnyliukh N, Johnson AJ, Nagel M, Monzer A, Babic D, Hlavata A, Alotaibi S,
Isono E, Loose M, Friml J. 2024. Role of dynamin-related proteins 2 and SH3P2
in clathrin-mediated endocytosis in Arabidopsis thaliana. Journal of Cell Science.
137(8), jcs. 261720.
mla: Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in
Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” Journal of Cell Science,
vol. 137, no. 8, jcs. 261720, The Company of Biologists, 2024, doi:10.1242/jcs.261720.
short: N. Gnyliukh, A.J. Johnson, M. Nagel, A. Monzer, D. Babic, A. Hlavata, S.
Alotaibi, E. Isono, M. Loose, J. Friml, Journal of Cell Science 137 (2024).
corr_author: '1'
date_created: 2024-04-19T09:54:59Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2025-09-04T13:49:45Z
day: '01'
ddc:
- '570'
department:
- _id: MaLo
- _id: JiFr
- _id: CaBe
doi: 10.1242/jcs.261720
ec_funded: 1
external_id:
isi:
- '001266917100005'
pmid:
- '38506228'
file:
- access_level: open_access
checksum: 6dc023f0cc7052ad3cf0a42589d2e30f
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T08:41:16Z
date_updated: 2025-01-09T08:41:16Z
file_id: '18792'
file_name: 2024_JourCellScience_Gnyliukh.pdf
file_size: 25845948
relation: main_file
success: 1
file_date_updated: 2025-01-09T08:41:16Z
has_accepted_license: '1'
intvolume: ' 137'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14591'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis
in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 137
year: '2024'
...
---
OA_place: publisher
OA_type: free access
_id: '14082'
abstract:
- lang: eng
text: Epithelial barrier function is commonly analyzed using transepithelial electrical
resistance, which measures ion flux across a monolayer, or by adding traceable
macromolecules and monitoring their passage across the monolayer. Although these
methods measure changes in global barrier function, they lack the sensitivity
needed to detect local or transient barrier breaches, and they do not reveal the
location of barrier leaks. Therefore, we previously developed a method that we
named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which
overcomes these limitations, allowing for detection of local tight junction leaks
with high spatiotemporal resolution. Here, we present expanded applications for
ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier
restoration and actin accumulation following laser injury. ZnUMBA can also be
effectively utilized in developing zebrafish embryos as well as cultured monolayers
of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful
and flexible method that, with minimal optimization, can be applied to multiple
systems to measure dynamic changes in barrier function with spatiotemporal precision.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "The authors thank their respective lab members for feedback and
helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria
for their support.\r\nThis work was supported by the National Institutes of Health
[R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan
Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for
Biomedical Engineering Research from the Nakatani Foundation for Advancement of
Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the
European Research Council [advanced grant 742573 to C.-P.H.]. "
article_number: jcs260668
article_processing_charge: No
article_type: original
author:
- first_name: Tomohito
full_name: Higashi, Tomohito
last_name: Higashi
- first_name: Rachel E.
full_name: Stephenson, Rachel E.
last_name: Stephenson
- first_name: Cornelia
full_name: Schwayer, Cornelia
id: 3436488C-F248-11E8-B48F-1D18A9856A87
last_name: Schwayer
orcid: 0000-0001-5130-2226
- first_name: Karla
full_name: Huljev, Karla
id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
last_name: Huljev
- first_name: Atsuko Y.
full_name: Higashi, Atsuko Y.
last_name: Higashi
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Hideki
full_name: Chiba, Hideki
last_name: Chiba
- first_name: Ann L.
full_name: Miller, Ann L.
last_name: Miller
citation:
ama: Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method
to detect local barrier breaches. Journal of Cell Science. 2023;136(15).
doi:10.1242/jcs.260668
apa: Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg,
C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local
barrier breaches. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.260668
chicago: Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev,
Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller.
“ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” Journal
of Cell Science. The Company of Biologists, 2023. https://doi.org/10.1242/jcs.260668.
ieee: T. Higashi et al., “ZnUMBA - a live imaging method to detect local
barrier breaches,” Journal of Cell Science, vol. 136, no. 15. The Company
of Biologists, 2023.
ista: Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ,
Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier
breaches. Journal of Cell Science. 136(15), jcs260668.
mla: Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier
Breaches.” Journal of Cell Science, vol. 136, no. 15, jcs260668, The Company
of Biologists, 2023, doi:10.1242/jcs.260668.
short: T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J.
Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).
date_created: 2023-08-20T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2025-06-25T06:28:45Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: EvBe
doi: 10.1242/jcs.260668
ec_funded: 1
external_id:
isi:
- '001070149000001'
pmid:
- '37461809'
has_accepted_license: '1'
intvolume: ' 136'
isi: 1
issue: '15'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/jcs.260668
month: '08'
oa: 1
oa_version: None
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: ZnUMBA - a live imaging method to detect local barrier breaches
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 136
year: '2023'
...
---
_id: '14316'
abstract:
- lang: eng
text: Clathrin-mediated vesicle trafficking plays central roles in post-Golgi transport.
In yeast (Saccharomyces cerevisiae), the AP-1 complex and GGA adaptors are predicted
to generate distinct transport vesicles at the trans-Golgi network (TGN), and
the epsin-related proteins Ent3p and Ent5p (collectively Ent3p/5p) act as accessories
for these adaptors. Recently, we showed that vesicle transport from the TGN is
crucial for yeast Rab5 (Vps21p)-mediated endosome formation, and that Ent3p/5p
are crucial for this process, whereas AP-1 and GGA adaptors are dispensable. However,
these observations were incompatible with previous studies showing that these
adaptors are required for Ent3p/5p recruitment to the TGN, and thus the overall
mechanism responsible for regulation of Vps21p activity remains ambiguous. Here,
we investigated the functional relationships between clathrin adaptors in post-Golgi-mediated
Vps21p activation. We show that AP-1 disruption in the ent3Δ5Δ mutant impaired
transport of the Vps21p guanine nucleotide exchange factor Vps9p transport to
the Vps21p compartment and severely reduced Vps21p activity. Additionally, GGA
adaptors, the phosphatidylinositol-4-kinase Pik1p and Rab11 GTPases Ypt31p and
Ypt32p were found to have partially overlapping functions for recruitment of AP-1
and Ent3p/5p to the TGN. These findings suggest a distinct role of clathrin adaptors
for Vps21p activation in the TGN–endosome trafficking pathway.
article_number: jcs261448
article_processing_charge: No
article_type: original
author:
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Kaito
full_name: Aoshima, Kaito
last_name: Aoshima
- first_name: Hiroki
full_name: Shimamura, Hiroki
last_name: Shimamura
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Junko Y.
full_name: Toshima, Junko Y.
last_name: Toshima
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Nagano M, Aoshima K, Shimamura H, Siekhaus DE, Toshima JY, Toshima J. Distinct
role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome
trafficking pathway. Journal of Cell Science. 2023;136(17). doi:10.1242/jcs.261448
apa: Nagano, M., Aoshima, K., Shimamura, H., Siekhaus, D. E., Toshima, J. Y., &
Toshima, J. (2023). Distinct role of TGN-resident clathrin adaptors for Vps21p
activation in the TGN-endosome trafficking pathway. Journal of Cell Science.
The Company of Biologists. https://doi.org/10.1242/jcs.261448
chicago: Nagano, Makoto, Kaito Aoshima, Hiroki Shimamura, Daria E Siekhaus, Junko
Y. Toshima, and Jiro Toshima. “Distinct Role of TGN-Resident Clathrin Adaptors
for Vps21p Activation in the TGN-Endosome Trafficking Pathway.” Journal of
Cell Science. The Company of Biologists, 2023. https://doi.org/10.1242/jcs.261448.
ieee: M. Nagano, K. Aoshima, H. Shimamura, D. E. Siekhaus, J. Y. Toshima, and J.
Toshima, “Distinct role of TGN-resident clathrin adaptors for Vps21p activation
in the TGN-endosome trafficking pathway,” Journal of Cell Science, vol.
136, no. 17. The Company of Biologists, 2023.
ista: Nagano M, Aoshima K, Shimamura H, Siekhaus DE, Toshima JY, Toshima J. 2023.
Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome
trafficking pathway. Journal of Cell Science. 136(17), jcs261448.
mla: Nagano, Makoto, et al. “Distinct Role of TGN-Resident Clathrin Adaptors for
Vps21p Activation in the TGN-Endosome Trafficking Pathway.” Journal of Cell
Science, vol. 136, no. 17, jcs261448, The Company of Biologists, 2023, doi:10.1242/jcs.261448.
short: M. Nagano, K. Aoshima, H. Shimamura, D.E. Siekhaus, J.Y. Toshima, J. Toshima,
Journal of Cell Science 136 (2023).
date_created: 2023-09-10T22:01:12Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2025-09-09T12:56:46Z
day: '01'
department:
- _id: DaSi
doi: 10.1242/jcs.261448
external_id:
isi:
- '001112832300005'
pmid:
- '37539494'
intvolume: ' 136'
isi: 1
issue: '17'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2023.03.27.534325
month: '09'
oa: 1
oa_version: Preprint
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the
TGN-endosome trafficking pathway
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 136
year: '2023'
...
---
_id: '14827'
abstract:
- lang: eng
text: Understanding complex living systems, which are fundamentally constrained
by physical phenomena, requires combining experimental data with theoretical physical
and mathematical models. To develop such models, collaborations between experimental
cell biologists and theoreticians are increasingly important but these two groups
often face challenges achieving mutual understanding. To help navigate these challenges,
this Perspective discusses different modelling approaches, including bottom-up
hypothesis-driven and top-down data-driven models, and highlights their strengths
and applications. Using cell mechanics as an example, we explore the integration
of specific physical models with experimental data from the molecular, cellular
and tissue level up to multiscale input. We also emphasize the importance of constraining
model complexity and outline strategies for crosstalk between experimental design
and model development. Furthermore, we highlight how physical models can provide
conceptual insights and produce unifying and generalizable frameworks for biological
phenomena. Overall, this Perspective aims to promote fruitful collaborations that
advance our understanding of complex biological systems.
acknowledgement: "We thank Prisca Liberali and Edouard Hannezo for many inspiring
discussions; Mehmet Can Uçar, Nicoletta I Petridou and Qiutan Yang for a critical
reading of the manuscript, and Claudia Flandoli for the artwork in Figs 2 and 3.
We would also like to thank The Company of Biologists for the opportunity to attend
the 2023 workshop on Collective Cell Migration, and all workshop participants for
discussions.\r\nC.S. was supported by a European Molecular Biology Organization
(EMBO) Postdoctoral Fellowship (ALTF 660-2020) and Human Frontier Science Program
(HFSP) Postdoctoral fellowship (LT000746/2021-L). D.B.B. was supported by the NOMIS
Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022)."
article_number: jcs.261515
article_processing_charge: No
article_type: original
author:
- first_name: Cornelia
full_name: Schwayer, Cornelia
id: 3436488C-F248-11E8-B48F-1D18A9856A87
last_name: Schwayer
orcid: 0000-0001-5130-2226
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
citation:
ama: Schwayer C, Brückner D. Connecting theory and experiment in cell and tissue
mechanics. Journal of Cell Science. 2023;136(24). doi:10.1242/jcs.261515
apa: Schwayer, C., & Brückner, D. (2023). Connecting theory and experiment in
cell and tissue mechanics. Journal of Cell Science. The Company of Biologists.
https://doi.org/10.1242/jcs.261515
chicago: Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment
in Cell and Tissue Mechanics.” Journal of Cell Science. The Company of
Biologists, 2023. https://doi.org/10.1242/jcs.261515.
ieee: C. Schwayer and D. Brückner, “Connecting theory and experiment in cell and
tissue mechanics,” Journal of Cell Science, vol. 136, no. 24. The Company
of Biologists, 2023.
ista: Schwayer C, Brückner D. 2023. Connecting theory and experiment in cell and
tissue mechanics. Journal of Cell Science. 136(24), jcs. 261515.
mla: Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment in
Cell and Tissue Mechanics.” Journal of Cell Science, vol. 136, no. 24,
jcs. 261515, The Company of Biologists, 2023, doi:10.1242/jcs.261515.
short: C. Schwayer, D. Brückner, Journal of Cell Science 136 (2023).
corr_author: '1'
date_created: 2024-01-17T12:46:55Z
date_published: 2023-12-27T00:00:00Z
date_updated: 2025-09-09T14:22:02Z
day: '27'
department:
- _id: EdHa
- _id: CaHe
doi: 10.1242/jcs.261515
external_id:
isi:
- '001165394900011'
pmid:
- '38149871'
intvolume: ' 136'
isi: 1
issue: '24'
keyword:
- Cell Biology
language:
- iso: eng
month: '12'
oa_version: None
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Connecting theory and experiment in cell and tissue mechanics
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 136
year: '2023'
...
---
_id: '12282'
abstract:
- lang: eng
text: From a simple thought to a multicellular movement
acknowledgement: The authors want to thank Professors Carrie Bernecky, Tom Henzinger,
Martin Loose and Gaia Novarino for accepting to be interviewed, thus giving significant
contribution to the discussion that lead to this article.
article_number: '260017'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: Irene
full_name: Vercellino, Irene
id: 3ED6AF16-F248-11E8-B48F-1D18A9856A87
last_name: Vercellino
orcid: 0000-0001-5618-3449
citation:
ama: Amberg N, Stouffer MA, Vercellino I. Operation STEM fatale – how an equity,
diversity and inclusion initiative has brought us to reflect on the current challenges
in cell biology and science as a whole. Journal of Cell Science. 2022;135(8).
doi:10.1242/jcs.260017
apa: Amberg, N., Stouffer, M. A., & Vercellino, I. (2022). Operation STEM fatale
– how an equity, diversity and inclusion initiative has brought us to reflect
on the current challenges in cell biology and science as a whole. Journal of
Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.260017
chicago: Amberg, Nicole, Melissa A Stouffer, and Irene Vercellino. “Operation STEM
Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect
on the Current Challenges in Cell Biology and Science as a Whole.” Journal
of Cell Science. The Company of Biologists, 2022. https://doi.org/10.1242/jcs.260017.
ieee: N. Amberg, M. A. Stouffer, and I. Vercellino, “Operation STEM fatale – how
an equity, diversity and inclusion initiative has brought us to reflect on the
current challenges in cell biology and science as a whole,” Journal of Cell
Science, vol. 135, no. 8. The Company of Biologists, 2022.
ista: Amberg N, Stouffer MA, Vercellino I. 2022. Operation STEM fatale – how an
equity, diversity and inclusion initiative has brought us to reflect on the current
challenges in cell biology and science as a whole. Journal of Cell Science. 135(8),
260017.
mla: Amberg, Nicole, et al. “Operation STEM Fatale – How an Equity, Diversity and
Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell
Biology and Science as a Whole.” Journal of Cell Science, vol. 135, no.
8, 260017, The Company of Biologists, 2022, doi:10.1242/jcs.260017.
short: N. Amberg, M.A. Stouffer, I. Vercellino, Journal of Cell Science 135 (2022).
corr_author: '1'
date_created: 2023-01-16T10:03:14Z
date_published: 2022-04-19T00:00:00Z
date_updated: 2024-10-09T21:03:55Z
day: '19'
department:
- _id: SiHi
- _id: LeSa
doi: 10.1242/jcs.260017
external_id:
isi:
- '000798123600015'
pmid:
- '35438168'
intvolume: ' 135'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa_version: None
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Operation STEM fatale – how an equity, diversity and inclusion initiative has
brought us to reflect on the current challenges in cell biology and science as a
whole
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 135
year: '2022'
...
---
_id: '12283'
abstract:
- lang: eng
text: Neurons extend axons to form the complex circuitry of the mature brain. This
depends on the coordinated response and continuous remodelling of the microtubule
and F-actin networks in the axonal growth cone. Growth cone architecture remains
poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron
growth cones using cryo-electron tomography to directly visualise their three-dimensional
subcellular architecture with molecular detail. Our data showed that the hexagonal
arrays of actin bundles that form filopodia penetrate and terminate deep within
the growth cone interior. We directly observed the modulation of these and other
growth cone actin bundles by alteration of individual F-actin helical structures.
Microtubules with blunt, slightly flared or gently curved ends predominated in
the growth cone, frequently contained lumenal particles and exhibited lattice
defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental
cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies
in overall growth cone organisation or in F-actin subpopulations. However, our
data suggested that microtubules sustained more structural defects, highlighting
the importance of microtubule integrity during growth cone migration.
acknowledgement: "J.A. was supported by a grant from the Medical Research Council
(MRC), UK (MR/R000352/1) to C.A.M. Cryo-EM data were collected on equipment funded
by the Wellcome Trust, UK (079605/Z/06/Z) and the Biotechnology and Biological Sciences
Research Council (BBSRC) UK (BB/L014211/1). F.F.’s salary and institute were supported
by Inserm (Institut National de la Santé et de la Recherche Médicale), CNRS (Centre
National de la Recherche Scientifique) and Sorbonne Université. F.F.’s group was
particularly supported by Agence Nationale de la\r\nRecherche (ANR-16-CE16-0011-03)
and Seventh Framework Programme (EUHEALTH-\r\n2013, DESIRE, N° 60253; also funding
M.S.’s salary) and the European Cooperation in Science and Technology (COST Action
CA16118). Open Access funding provided by Birkbeck College: Birkbeck University
of London. Deposited in PMC for immediate release."
article_number: '259234'
article_processing_charge: No
article_type: original
author:
- first_name: Joseph
full_name: Atherton, Joseph
last_name: Atherton
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: Fiona
full_name: Francis, Fiona
last_name: Francis
- first_name: Carolyn A.
full_name: Moores, Carolyn A.
last_name: Moores
citation:
ama: Atherton J, Stouffer MA, Francis F, Moores CA. Visualising the cytoskeletal
machinery in neuronal growth cones using cryo-electron tomography. Journal
of Cell Science. 2022;135(7). doi:10.1242/jcs.259234
apa: Atherton, J., Stouffer, M. A., Francis, F., & Moores, C. A. (2022). Visualising
the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography.
Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.259234
chicago: Atherton, Joseph, Melissa A Stouffer, Fiona Francis, and Carolyn A. Moores.
“Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron
Tomography.” Journal of Cell Science. The Company of Biologists, 2022.
https://doi.org/10.1242/jcs.259234.
ieee: J. Atherton, M. A. Stouffer, F. Francis, and C. A. Moores, “Visualising the
cytoskeletal machinery in neuronal growth cones using cryo-electron tomography,”
Journal of Cell Science, vol. 135, no. 7. The Company of Biologists, 2022.
ista: Atherton J, Stouffer MA, Francis F, Moores CA. 2022. Visualising the cytoskeletal
machinery in neuronal growth cones using cryo-electron tomography. Journal of
Cell Science. 135(7), 259234.
mla: Atherton, Joseph, et al. “Visualising the Cytoskeletal Machinery in Neuronal
Growth Cones Using Cryo-Electron Tomography.” Journal of Cell Science,
vol. 135, no. 7, 259234, The Company of Biologists, 2022, doi:10.1242/jcs.259234.
short: J. Atherton, M.A. Stouffer, F. Francis, C.A. Moores, Journal of Cell Science
135 (2022).
date_created: 2023-01-16T10:03:24Z
date_published: 2022-04-01T00:00:00Z
date_updated: 2023-08-04T10:28:34Z
day: '01'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1242/jcs.259234
external_id:
isi:
- '000783840400010'
pmid:
- '35383828'
file:
- access_level: open_access
checksum: 4346ed32cb7c89a8ca051c7da68a9a1c
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:41:01Z
date_updated: 2023-01-30T11:41:01Z
file_id: '12461'
file_name: 2022_JourCellBiology_Atherton.pdf
file_size: 13868733
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:41:01Z
has_accepted_license: '1'
intvolume: ' 135'
isi: 1
issue: '7'
keyword:
- Cell Biology
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron
tomography
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 135
year: '2022'
...
---
_id: '17057'
abstract:
- lang: eng
text: Martin Loose studied chemistry at the University of Heidelberg, Germany. He
then joined Petra Schwille's group at the Max Planck Institute of Molecular Cell
Biology and Genetics in Dresden, where he obtained his PhD degree in 2010 for
work on self-organization and pattern formation in the bacterial Min protein system.
He then moved to Tim Mitchison's lab at Harvard Medical School, Boston, USA for
his postdoc, funded by Human Frontier Science Program (HSFP) and European Molecular
Biology Organization (EMBO) long-term fellowships; there, he discovered that the
bacterial cell division proteins FtsA and FtsZ self-organize into dynamic cytoskeletal
patterns. Martin established his independent research group at the Institute of
Science and Technology (IST) Austria in 2015, supported by an European Research
Council (ERC) starting grant and HFSP Young Investigator Grant. His lab studies
the self-organization of bacterial cell division and small GTPase networks.
article_number: jcs259715
article_processing_charge: No
author:
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
citation:
ama: Loose M. Cell Scientist to Watch – Martin Loose. Vol 135. The Company
of Biologists; 2022. doi:10.1242/jcs.259715
apa: Loose, M. (2022). Cell scientist to watch – Martin Loose. Journal
of Cell Science (Vol. 135). The Company of Biologists. https://doi.org/10.1242/jcs.259715
chicago: Loose, Martin. Cell Scientist to Watch – Martin Loose. Journal
of Cell Science. Vol. 135. The Company of Biologists, 2022. https://doi.org/10.1242/jcs.259715.
ieee: M. Loose, Cell scientist to watch – Martin Loose, vol. 135, no. 2.
The Company of Biologists, 2022.
ista: Loose M. 2022. Cell scientist to watch – Martin Loose, The Company of Biologists,p.
mla: Loose, Martin. “Cell Scientist to Watch – Martin Loose.” Journal of Cell
Science, vol. 135, no. 2, jcs259715, The Company of Biologists, 2022, doi:10.1242/jcs.259715.
short: M. Loose, Cell Scientist to Watch – Martin Loose, The Company of Biologists,
2022.
date_created: 2024-05-28T13:28:30Z
date_published: 2022-01-19T00:00:00Z
date_updated: 2026-06-18T17:51:26Z
day: '19'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1242/jcs.259715
external_id:
isi:
- '000762665200015'
intvolume: ' 135'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/jcs.259715
month: '01'
oa: 1
oa_version: Published Version
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
status: public
title: Cell scientist to watch – Martin Loose
type: other_academic_publication
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 135
year: '2022'
...
---
_id: '9952'
abstract:
- lang: eng
text: Proper control of division orientation and symmetry, largely determined by
spindle positioning, is essential to development and homeostasis. Spindle positioning
has been extensively studied in cells dividing in two-dimensional (2D) environments
and in epithelial tissues, where proteins such as NuMA (also known as NUMA1) orient
division along the interphase long axis of the cell. However, little is known
about how cells control spindle positioning in three-dimensional (3D) environments,
such as early mammalian embryos and a variety of adult tissues. Here, we use mouse
embryonic stem cells (ESCs), which grow in 3D colonies, as a model to investigate
division in 3D. We observe that, at the periphery of 3D colonies, ESCs display
high spindle mobility and divide asymmetrically. Our data suggest that enhanced
spindle movements are due to unequal distribution of the cell–cell junction protein
E-cadherin between future daughter cells. Interestingly, when cells progress towards
differentiation, division becomes more symmetric, with more elongated shapes in
metaphase and enhanced cortical NuMA recruitment in anaphase. Altogether, this
study suggests that in 3D contexts, the geometry of the cell and its contacts
with neighbors control division orientation and symmetry.
acknowledgement: We would like to thank the entire Paluch and Baum laboratories at
the MRC-LMCB and the Chalut lab at the Cambridge SCI for discussions and feedback
throughout the project, and the MRC-LMCB microscopy platform, in particular Andrew
Vaughan, for technical support.
article_number: jcs255018
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Agathe
full_name: Chaigne, Agathe
last_name: Chaigne
- first_name: Matthew B.
full_name: Smith, Matthew B.
last_name: Smith
- first_name: R. L.
full_name: Cavestany, R. L.
last_name: Cavestany
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Kevin J.
full_name: Chalut, Kevin J.
last_name: Chalut
- first_name: Ewa K.
full_name: Paluch, Ewa K.
last_name: Paluch
citation:
ama: Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. Three-dimensional
geometry controls division symmetry in stem cell colonies. Journal of Cell
Science. 2021;134(14). doi:10.1242/jcs.255018
apa: Chaigne, A., Smith, M. B., Cavestany, R. L., Hannezo, E. B., Chalut, K. J.,
& Paluch, E. K. (2021). Three-dimensional geometry controls division symmetry
in stem cell colonies. Journal of Cell Science. The Company of Biologists.
https://doi.org/10.1242/jcs.255018
chicago: Chaigne, Agathe, Matthew B. Smith, R. L. Cavestany, Edouard B Hannezo,
Kevin J. Chalut, and Ewa K. Paluch. “Three-Dimensional Geometry Controls Division
Symmetry in Stem Cell Colonies.” Journal of Cell Science. The Company of
Biologists, 2021. https://doi.org/10.1242/jcs.255018.
ieee: A. Chaigne, M. B. Smith, R. L. Cavestany, E. B. Hannezo, K. J. Chalut, and
E. K. Paluch, “Three-dimensional geometry controls division symmetry in stem cell
colonies,” Journal of Cell Science, vol. 134, no. 14. The Company of Biologists,
2021.
ista: Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. 2021.
Three-dimensional geometry controls division symmetry in stem cell colonies. Journal
of Cell Science. 134(14), jcs255018.
mla: Chaigne, Agathe, et al. “Three-Dimensional Geometry Controls Division Symmetry
in Stem Cell Colonies.” Journal of Cell Science, vol. 134, no. 14, jcs255018,
The Company of Biologists, 2021, doi:10.1242/jcs.255018.
short: A. Chaigne, M.B. Smith, R.L. Cavestany, E.B. Hannezo, K.J. Chalut, E.K. Paluch,
Journal of Cell Science 134 (2021).
date_created: 2021-08-22T22:01:20Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2025-07-10T12:02:07Z
day: '01'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1242/jcs.255018
external_id:
isi:
- '000681395800008'
file:
- access_level: open_access
checksum: f086f9d7cb63b2474c01921cb060c513
content_type: application/pdf
creator: asandaue
date_created: 2021-08-23T07:32:20Z
date_updated: 2021-08-23T07:32:20Z
file_id: '9954'
file_name: 2021_JournalOfCellScience_Chaigne.pdf
file_size: 8651724
relation: main_file
success: 1
file_date_updated: 2021-08-23T07:32:20Z
has_accepted_license: '1'
intvolume: ' 134'
isi: 1
issue: '14'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Three-dimensional geometry controls division symmetry in stem cell colonies
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 134
year: '2021'
...
---
_id: '8434'
abstract:
- lang: eng
text: 'Efficient migration on adhesive surfaces involves the protrusion of lamellipodial
actin networks and their subsequent stabilization by nascent adhesions. The actin-binding
protein lamellipodin (Lpd) is thought to play a critical role in lamellipodium
protrusion, by delivering Ena/VASP proteins onto the growing plus ends of actin
filaments and by interacting with the WAVE regulatory complex, an activator of
the Arp2/3 complex, at the leading edge. Using B16-F1 melanoma cell lines, we
demonstrate that genetic ablation of Lpd compromises protrusion efficiency and
coincident cell migration without altering essential parameters of lamellipodia,
including their maximal rate of forward advancement and actin polymerization.
We also confirmed lamellipodia and migration phenotypes with CRISPR/Cas9-mediated
Lpd knockout Rat2 fibroblasts, excluding cell type-specific effects. Moreover,
computer-aided analysis of cell-edge morphodynamics on B16-F1 cell lamellipodia
revealed that loss of Lpd correlates with reduced temporal protrusion maintenance
as a prerequisite of nascent adhesion formation. We conclude that Lpd optimizes
protrusion and nascent adhesion formation by counteracting frequent, chaotic retraction
and membrane ruffling.This article has an associated First Person interview with
the first author of the paper. '
acknowledgement: This work was supported in part by Deutsche Forschungsgemeinschaft
(DFG)[GRK2223/1, RO2414/5-1 (to K.R.), FA350/11-1 (to M.F.) and FA330/11-1 (to J.F.)],as
well as by intramural funding from the Helmholtz Association (to T.E.B.S. andK.R.).
G.D. was additionally funded by the Austrian Science Fund (FWF) LiseMeitner Program
[M-2495]. A.C.H. and M.W. are supported by the Francis CrickInstitute, which receives
its core funding from Cancer Research UK [FC001209], theMedical Research Council
[FC001209] and the Wellcome Trust [FC001209]. M.K. issupported by the Biotechnology
and Biological Sciences Research Council [BB/F011431/1, BB/J000590/1, BB/N000226/1].
Deposited in PMC for release after 6months.
article_number: jcs239020
article_processing_charge: No
article_type: original
author:
- first_name: Georgi A
full_name: Dimchev, Georgi A
id: 38C393BE-F248-11E8-B48F-1D18A9856A87
last_name: Dimchev
orcid: 0000-0001-8370-6161
- first_name: Behnam
full_name: Amiri, Behnam
last_name: Amiri
- first_name: Ashley C.
full_name: Humphries, Ashley C.
last_name: Humphries
- first_name: Matthias
full_name: Schaks, Matthias
last_name: Schaks
- first_name: Vanessa
full_name: Dimchev, Vanessa
last_name: Dimchev
- first_name: Theresia E. B.
full_name: Stradal, Theresia E. B.
last_name: Stradal
- first_name: Jan
full_name: Faix, Jan
last_name: Faix
- first_name: Matthias
full_name: Krause, Matthias
last_name: Krause
- first_name: Michael
full_name: Way, Michael
last_name: Way
- first_name: Martin
full_name: Falcke, Martin
last_name: Falcke
- first_name: Klemens
full_name: Rottner, Klemens
last_name: Rottner
citation:
ama: Dimchev GA, Amiri B, Humphries AC, et al. Lamellipodin tunes cell migration
by stabilizing protrusions and promoting adhesion formation. Journal of Cell
Science. 2020;133(7). doi:10.1242/jcs.239020
apa: Dimchev, G. A., Amiri, B., Humphries, A. C., Schaks, M., Dimchev, V., Stradal,
T. E. B., … Rottner, K. (2020). Lamellipodin tunes cell migration by stabilizing
protrusions and promoting adhesion formation. Journal of Cell Science.
The Company of Biologists. https://doi.org/10.1242/jcs.239020
chicago: Dimchev, Georgi A, Behnam Amiri, Ashley C. Humphries, Matthias Schaks,
Vanessa Dimchev, Theresia E. B. Stradal, Jan Faix, et al. “Lamellipodin Tunes
Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation.” Journal
of Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.239020.
ieee: G. A. Dimchev et al., “Lamellipodin tunes cell migration by stabilizing
protrusions and promoting adhesion formation,” Journal of Cell Science,
vol. 133, no. 7. The Company of Biologists, 2020.
ista: Dimchev GA, Amiri B, Humphries AC, Schaks M, Dimchev V, Stradal TEB, Faix
J, Krause M, Way M, Falcke M, Rottner K. 2020. Lamellipodin tunes cell migration
by stabilizing protrusions and promoting adhesion formation. Journal of Cell Science.
133(7), jcs239020.
mla: Dimchev, Georgi A., et al. “Lamellipodin Tunes Cell Migration by Stabilizing
Protrusions and Promoting Adhesion Formation.” Journal of Cell Science,
vol. 133, no. 7, jcs239020, The Company of Biologists, 2020, doi:10.1242/jcs.239020.
short: G.A. Dimchev, B. Amiri, A.C. Humphries, M. Schaks, V. Dimchev, T.E.B. Stradal,
J. Faix, M. Krause, M. Way, M. Falcke, K. Rottner, Journal of Cell Science 133
(2020).
date_created: 2020-09-17T14:00:33Z
date_published: 2020-04-09T00:00:00Z
date_updated: 2025-04-15T07:52:13Z
day: '09'
ddc:
- '570'
department:
- _id: FlSc
doi: 10.1242/jcs.239020
external_id:
isi:
- '000534387800005'
pmid:
- ' 32094266'
file:
- access_level: open_access
checksum: ba917e551acc4ece2884b751434df9ae
content_type: application/pdf
creator: dernst
date_created: 2020-09-17T14:07:51Z
date_updated: 2020-10-11T22:30:02Z
embargo: 2020-10-10
file_id: '8435'
file_name: 2020_JournalCellScience_Dimchev.pdf
file_size: 13493302
relation: main_file
file_date_updated: 2020-10-11T22:30:02Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '7'
keyword:
- Cell Biology
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2674F658-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02495
name: Protein structure and function in filopodia across scales
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Lamellipodin tunes cell migration by stabilizing protrusions and promoting
adhesion formation
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '8139'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated
in many aspects of plant growth, development, intra- and inter-cellular signaling,
nutrient uptake and pathogen defense. Despite these significant roles, little
is known about the precise molecular details of how it functions in planta. In
order to facilitate the direct quantitative study of plant CME, here we review
current routinely used methods and present refined, standardized quantitative
imaging protocols which allow the detailed characterization of CME at multiple
scales in plant tissues. These include: (i) an efficient electron microscopy protocol
for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for
the detailed characterization of the ultra-structure of clathrin-coated vesicles;
(ii) a detailed protocol and analysis for quantitative live-cell fluorescence
microscopy to precisely examine the temporal interplay of endocytosis components
during single CME events; (iii) a semi-automated analysis to allow the quantitative
characterization of global internalization of cargos in whole plant tissues; and
(iv) an overview and validation of useful genetic and pharmacological tools to
interrogate the molecular mechanisms and function of CME in intact plant samples.'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
acknowledgement: "This paper is dedicated to the memory of Christien Merrifield. He
pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship
inspired the development of all\r\nthe analysis methods presented here. His joy
in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant
inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP
plants used in this manuscript. We further thank the\r\nScientific Service Units
at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical
assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility
for access to equipment. "
article_number: jcs248062
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative
evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal
of Cell Science. 2020;133(15). doi:10.1242/jcs.248062
apa: Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek,
S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of
clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell
Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062
chicago: Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan,
G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062.
ieee: A. J. Johnson et al., “Experimental toolbox for quantitative evaluation
of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of
Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.
ista: Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml
J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated
endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15),
jcs248062.
mla: Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020,
doi:10.1242/jcs.248062.
short: A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek,
J. Friml, Journal of Cell Science 133 (2020).
date_created: 2020-07-21T08:58:19Z
date_published: 2020-08-06T00:00:00Z
date_updated: 2026-06-19T22:30:32Z
day: '06'
ddc:
- '575'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1242/jcs.248062
ec_funded: 1
external_id:
isi:
- '000561047900021'
pmid:
- '32616560'
file:
- access_level: open_access
checksum: 2d11f79a0b4e0a380fb002b933da331a
content_type: application/pdf
creator: ajohnson
date_created: 2020-11-26T17:12:51Z
date_updated: 2021-08-08T22:30:03Z
embargo: 2021-08-07
file_id: '8815'
file_name: 2020 - Johnson - JSC - plant CME toolbox.pdf
file_size: 15150403
relation: main_file
file_date_updated: 2021-08-08T22:30:03Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis
in the plant model Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '7420'
abstract:
- lang: eng
text: β1-integrins mediate cell–matrix interactions and their trafficking is important
in the dynamic regulation of cell adhesion, migration and malignant processes,
including cancer cell invasion. Here, we employ an RNAi screen to characterize
regulators of integrin traffic and identify the association of Golgi-localized
gamma ear-containing Arf-binding protein 2 (GGA2) with β1-integrin, and its role
in recycling of active but not inactive β1-integrin receptors. Silencing of GGA2
limits active β1-integrin levels in focal adhesions and decreases cancer cell
migration and invasion, which is in agreement with its ability to regulate the
dynamics of active integrins. By using the proximity-dependent biotin identification
(BioID) method, we identified two RAB family small GTPases, i.e. RAB13 and RAB10,
as novel interactors of GGA2. Functionally, RAB13 silencing triggers the intracellular
accumulation of active β1-integrin, and reduces integrin activity in focal adhesions
and cell migration similarly to GGA2 depletion, indicating that both facilitate
active β1-integrin recycling to the plasma membrane. Thus, GGA2 and RAB13 are
important specificity determinants for integrin activity-dependent traffic.
article_number: jcs233387
article_processing_charge: No
article_type: original
author:
- first_name: Pranshu
full_name: Sahgal, Pranshu
last_name: Sahgal
- first_name: Jonna H
full_name: Alanko, Jonna H
id: 2CC12E8C-F248-11E8-B48F-1D18A9856A87
last_name: Alanko
orcid: 0000-0002-7698-3061
- first_name: Jaroslav
full_name: Icha, Jaroslav
last_name: Icha
- first_name: Ilkka
full_name: Paatero, Ilkka
last_name: Paatero
- first_name: Hellyeh
full_name: Hamidi, Hellyeh
last_name: Hamidi
- first_name: Antti
full_name: Arjonen, Antti
last_name: Arjonen
- first_name: Mika
full_name: Pietilä, Mika
last_name: Pietilä
- first_name: Anne
full_name: Rokka, Anne
last_name: Rokka
- first_name: Johanna
full_name: Ivaska, Johanna
last_name: Ivaska
citation:
ama: Sahgal P, Alanko JH, Icha J, et al. GGA2 and RAB13 promote activity-dependent
β1-integrin recycling. Journal of Cell Science. 2019;132(11). doi:10.1242/jcs.233387
apa: Sahgal, P., Alanko, J. H., Icha, J., Paatero, I., Hamidi, H., Arjonen, A.,
… Ivaska, J. (2019). GGA2 and RAB13 promote activity-dependent β1-integrin recycling.
Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.233387
chicago: Sahgal, Pranshu, Jonna H Alanko, Jaroslav Icha, Ilkka Paatero, Hellyeh
Hamidi, Antti Arjonen, Mika Pietilä, Anne Rokka, and Johanna Ivaska. “GGA2 and
RAB13 Promote Activity-Dependent Β1-Integrin Recycling.” Journal of Cell Science.
The Company of Biologists, 2019. https://doi.org/10.1242/jcs.233387.
ieee: P. Sahgal et al., “GGA2 and RAB13 promote activity-dependent β1-integrin
recycling,” Journal of Cell Science, vol. 132, no. 11. The Company of Biologists,
2019.
ista: Sahgal P, Alanko JH, Icha J, Paatero I, Hamidi H, Arjonen A, Pietilä M, Rokka
A, Ivaska J. 2019. GGA2 and RAB13 promote activity-dependent β1-integrin recycling.
Journal of Cell Science. 132(11), jcs233387.
mla: Sahgal, Pranshu, et al. “GGA2 and RAB13 Promote Activity-Dependent Β1-Integrin
Recycling.” Journal of Cell Science, vol. 132, no. 11, jcs233387, The Company
of Biologists, 2019, doi:10.1242/jcs.233387.
short: P. Sahgal, J.H. Alanko, J. Icha, I. Paatero, H. Hamidi, A. Arjonen, M. Pietilä,
A. Rokka, J. Ivaska, Journal of Cell Science 132 (2019).
date_created: 2020-01-30T10:31:42Z
date_published: 2019-06-07T00:00:00Z
date_updated: 2026-06-18T19:21:00Z
day: '07'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1242/jcs.233387
external_id:
isi:
- '000473327900017'
pmid:
- '31076515'
intvolume: ' 132'
isi: 1
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/jcs.233387
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
status: public
title: GGA2 and RAB13 promote activity-dependent β1-integrin recycling
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 132
year: '2019'
...
---
_id: '11113'
abstract:
- lang: eng
text: The nuclear envelope (NE), a double membrane enclosing the nucleus of eukaryotic
cells, controls the flow of information between the nucleoplasm and the cytoplasm
and provides a scaffold for the organization of chromatin and the cytoskeleton.
In dividing metazoan cells, the NE breaks down at the onset of mitosis and then
reforms around segregated chromosomes to generate the daughter nuclei. Recent
data from intact cells and cell-free nuclear assembly systems suggest that the
endoplasmic reticulum (ER) is the source of membrane for NE assembly. At the end
of mitosis, ER membrane tubules are targeted to chromatin via tubule ends and
reorganized into flat nuclear membrane sheets by specific DNA-binding membrane
proteins. In contrast to previous models, which proposed vesicle fusion to be
the principal mechanism of NE formation, these new studies suggest that the nuclear
membrane forms by the chromatin-mediated reshaping of the ER.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Daniel J.
full_name: Anderson, Daniel J.
last_name: Anderson
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
citation:
ama: Anderson DJ, Hetzer M. Shaping the endoplasmic reticulum into the nuclear envelope.
Journal of Cell Science. 2008;121(2):137-142. doi:10.1242/jcs.005777
apa: Anderson, D. J., & Hetzer, M. (2008). Shaping the endoplasmic reticulum
into the nuclear envelope. Journal of Cell Science. The Company of Biologists.
https://doi.org/10.1242/jcs.005777
chicago: Anderson, Daniel J., and Martin Hetzer. “Shaping the Endoplasmic Reticulum
into the Nuclear Envelope.” Journal of Cell Science. The Company of Biologists,
2008. https://doi.org/10.1242/jcs.005777.
ieee: D. J. Anderson and M. Hetzer, “Shaping the endoplasmic reticulum into the
nuclear envelope,” Journal of Cell Science, vol. 121, no. 2. The Company
of Biologists, pp. 137–142, 2008.
ista: Anderson DJ, Hetzer M. 2008. Shaping the endoplasmic reticulum into the nuclear
envelope. Journal of Cell Science. 121(2), 137–142.
mla: Anderson, Daniel J., and Martin Hetzer. “Shaping the Endoplasmic Reticulum
into the Nuclear Envelope.” Journal of Cell Science, vol. 121, no. 2, The
Company of Biologists, 2008, pp. 137–42, doi:10.1242/jcs.005777.
short: D.J. Anderson, M. Hetzer, Journal of Cell Science 121 (2008) 137–142.
date_created: 2022-04-07T07:55:46Z
date_published: 2008-01-15T00:00:00Z
date_updated: 2024-10-14T11:29:47Z
day: '15'
doi: 10.1242/jcs.005777
extern: '1'
external_id:
pmid:
- '18187447'
intvolume: ' 121'
issue: '2'
keyword:
- Cell Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/jcs.005777
month: '01'
oa: 1
oa_version: Published Version
page: 137-142
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Shaping the endoplasmic reticulum into the nuclear envelope
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 121
year: '2008'
...
---
OA_place: repository
OA_type: green
_id: '4193'
abstract:
- lang: eng
text: The controlled adhesion of cells to each other and to the extracellular matrix
is crucial for tissue development and maintenance. Numerous assays have been developed
to quantify cell adhesion. Among these, the use of atomic force microscopy (AFM)
for single-cell force spectroscopy (SCFS) has recently been established. This
assay permits the adhesion of living cells to be studied in near-physiological
conditions. This implementation of AFM allows unrivaled spatial and temporal control
of cells, as well as highly quantitative force actuation and force measurement
that is sufficiently sensitive to characterize the interaction of single molecules.
Therefore, not only overall cell adhesion but also the properties of single adhesion-receptor-ligand
interactions can be studied. Here we describe current implementations and applications
of SCFS, as well as potential pitfalls, and outline how developments will provide
insight into the forces, energetics and kinetics of cell-adhesion processes.
article_processing_charge: No
article_type: comment
author:
- first_name: Jonne
full_name: Helenius, Jonne
last_name: Helenius
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Hermann
full_name: Gaub, Hermann
last_name: Gaub
- first_name: Daniel
full_name: Mueller, Daniel
last_name: Mueller
citation:
ama: Helenius J, Heisenberg C-PJ, Gaub H, Mueller D. Single-cell force spectroscopy.
Journal of Cell Science. 2008;121(11):1785-1791. doi:10.1242/jcs.030999
apa: Helenius, J., Heisenberg, C.-P. J., Gaub, H., & Mueller, D. (2008). Single-cell
force spectroscopy. Journal of Cell Science. Company of Biologists. https://doi.org/10.1242/jcs.030999
chicago: Helenius, Jonne, Carl-Philipp J Heisenberg, Hermann Gaub, and Daniel Mueller.
“Single-Cell Force Spectroscopy.” Journal of Cell Science. Company of Biologists,
2008. https://doi.org/10.1242/jcs.030999.
ieee: J. Helenius, C.-P. J. Heisenberg, H. Gaub, and D. Mueller, “Single-cell force
spectroscopy,” Journal of Cell Science, vol. 121, no. 11. Company of Biologists,
pp. 1785–1791, 2008.
ista: Helenius J, Heisenberg C-PJ, Gaub H, Mueller D. 2008. Single-cell force spectroscopy.
Journal of Cell Science. 121(11), 1785–1791.
mla: Helenius, Jonne, et al. “Single-Cell Force Spectroscopy.” Journal of Cell
Science, vol. 121, no. 11, Company of Biologists, 2008, pp. 1785–91, doi:10.1242/jcs.030999.
short: J. Helenius, C.-P.J. Heisenberg, H. Gaub, D. Mueller, Journal of Cell Science
121 (2008) 1785–1791.
date_created: 2018-12-11T12:07:30Z
date_published: 2008-06-01T00:00:00Z
date_updated: 2026-05-28T14:11:54Z
day: '01'
doi: 10.1242/jcs.030999
extern: '1'
external_id:
pmid:
- '18492792'
intvolume: ' 121'
issue: '11'
language:
- iso: eng
month: '06'
oa_version: None
page: 1785 - 1791
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: Company of Biologists
publist_id: '1924'
status: public
title: Single-cell force spectroscopy
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 121
year: '2008'
...