---
OA_place: repository
OA_type: green
_id: '21848'
abstract:
- lang: eng
  text: 'Despite the success of mRNA therapeutics, challenges remain in optimizing
    immune responses and minimizing side effects. Cell-specific antigen delivery may
    help reduce required doses and improve vaccine efficacy. In this study, we report
    on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting
    cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific
    glycomimetic ligand, we achieve selective mRNA delivery to both murine and human
    primary Langerhans cells with minimal off-target uptake, at the same time resulting
    in significantly increased mRNA translation. This targeted mRNA delivery not only
    enhances antigen presentation and T-cell responses but also enables dose-sparing
    and superior antitumor immunity compared with conventional immunization in a B16-OVA
    tumor model. Importantly, our platform’s high compatibility with various lipid
    nanoparticle formulations offers a flexible and precise tool for skin-directed
    mRNA delivery.'
acknowledged_ssus:
- _id: PreCl
acknowledgement: We thank Mareike Rentzsch for her intellectual contributions during
  the course of our discussions. We thank Michael Schunn from the Preclinical Facility
  of the Institute of Science and Technology Austria for his continuous technical
  support. Guarantor of the work is FS. This project was supported by “Seedfinancing”
  (P2282679) of the Austrian Federal Ministry of Digital and Economic Affairs and
  the Ministry of Climate Action and Energy, handled by the Austrian Wirtschaftsservice,
  as well as by...
article_processing_charge: No
article_type: original
author:
- first_name: Klara
  full_name: Klein, Klara
  last_name: Klein
- first_name: Litty
  full_name: Johnson, Litty
  last_name: Johnson
- first_name: Ramona
  full_name: Rîca, Ramona
  last_name: Rîca
- first_name: Mirza
  full_name: Sarcevic, Mirza
  last_name: Sarcevic
- first_name: Gabriele
  full_name: Carta, Gabriele
  last_name: Carta
- first_name: Saskia
  full_name: Seiser, Saskia
  last_name: Seiser
- first_name: Adelheid
  full_name: Elbe-Bürger, Adelheid
  last_name: Elbe-Bürger
- first_name: Freyja
  full_name: Langer, Freyja
  id: 3C1BE782-F248-11E8-B48F-1D18A9856A87
  last_name: Langer
- first_name: Nowras
  full_name: Rahhal, Nowras
  last_name: Rahhal
- first_name: Christoph
  full_name: Rademacher, Christoph
  last_name: Rademacher
- first_name: Robert
  full_name: Wawrzinek, Robert
  last_name: Wawrzinek
- first_name: Federica
  full_name: Quattrone, Federica
  last_name: Quattrone
- first_name: Florian
  full_name: Sparber, Florian
  last_name: Sparber
citation:
  ama: 'Klein K, Johnson L, Rîca R, et al. Langerhans cell–targeted mRNA delivery:
    A strategy for dose-sparing and enhanced antitumor immunity. <i>Journal of Investigative
    Dermatology</i>. doi:<a href="https://doi.org/10.1016/j.jid.2026.03.026">10.1016/j.jid.2026.03.026</a>'
  apa: 'Klein, K., Johnson, L., Rîca, R., Sarcevic, M., Carta, G., Seiser, S., … Sparber,
    F. (n.d.). Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing
    and enhanced antitumor immunity. <i>Journal of Investigative Dermatology</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.jid.2026.03.026">https://doi.org/10.1016/j.jid.2026.03.026</a>'
  chicago: 'Klein, Klara, Litty Johnson, Ramona Rîca, Mirza Sarcevic, Gabriele Carta,
    Saskia Seiser, Adelheid Elbe-Bürger, et al. “Langerhans Cell–Targeted MRNA Delivery:
    A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative
    Dermatology</i>. Elsevier, n.d. <a href="https://doi.org/10.1016/j.jid.2026.03.026">https://doi.org/10.1016/j.jid.2026.03.026</a>.'
  ieee: 'K. Klein <i>et al.</i>, “Langerhans cell–targeted mRNA delivery: A strategy
    for dose-sparing and enhanced antitumor immunity,” <i>Journal of Investigative
    Dermatology</i>. Elsevier.'
  ista: 'Klein K, Johnson L, Rîca R, Sarcevic M, Carta G, Seiser S, Elbe-Bürger A,
    Langer F, Rahhal N, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans
    cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor
    immunity. Journal of Investigative Dermatology.'
  mla: 'Klein, Klara, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for
    Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative Dermatology</i>,
    Elsevier, doi:<a href="https://doi.org/10.1016/j.jid.2026.03.026">10.1016/j.jid.2026.03.026</a>.'
  short: K. Klein, L. Johnson, R. Rîca, M. Sarcevic, G. Carta, S. Seiser, A. Elbe-Bürger,
    F. Langer, N. Rahhal, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Journal
    of Investigative Dermatology (n.d.).
date_created: 2026-05-10T22:02:16Z
date_published: 2026-04-07T00:00:00Z
date_updated: 2026-05-11T06:07:32Z
day: '07'
department:
- _id: PreCl
doi: 10.1016/j.jid.2026.03.026
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2025.06.25.661517
month: '04'
oa: 1
oa_version: Preprint
publication: Journal of Investigative Dermatology
publication_identifier:
  eissn:
  - 1523-1747
  issn:
  - 0022-202X
publication_status: inpress
publisher: Elsevier
scopus_import: '1'
status: public
title: 'Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced
  antitumor immunity'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19507'
abstract:
- lang: eng
  text: The epidermis provides a protective barrier against hostile environments.
    However, our knowledge of how this barrier forms during development and is subsequently
    maintained remains incomplete. The infundibulum is a cylindrical epidermal tissue
    compartment that serves as an outlet for hair follicles protruding from the skin
    and the excretion of the sebaceous glands that are essential for proper skin function.
    In this study, we applied quantitative fate mapping to address how infundibulum
    are maintained during adulthood. We demonstrate that progenitors build and maintain
    tissues through stochastic cell fate choices. Long-term analysis identified a
    preferential transient contribution from cells initially located at the bottom
    of the structure to the maintenance of the tissue, with bursts of local progenitor
    expansion associated with the phases of hair growth. Beyond providing compartment-wide
    insights into progenitor cell dynamics in infundibulum, these findings demonstrate
    how spatiotemporal regulation controls transient progenitor dominance.
acknowledgement: We thank the members of the Jensen Laboratory for experimental and
  technical advice, the imaging facilities at reNEW, and animal caretakers for expert
  assistance. This work was supported by the Lundbeck Foundation (R105-A9755 to KBJ)
  and the Leo Pharma Foundation (LF-OC-20-000169). The Novo Nordisk Foundation Center
  for Stem Cell Medicine was supported by a Novo Nordisk Foundation grant (NNF21CC0073729).
  B.D.S. was supported by the Wellcome Trust (219478/Z/19/Z) and a Royal Society EP
  Abraham Research Professorship (RP/R1/180165 and RP\R\231004). Figure elements were
  adapted from Bio-Render. KBJ is the lead contact and guarantor of this study.
article_processing_charge: No
article_type: original
author:
- first_name: Marianne S.
  full_name: Andersen, Marianne S.
  last_name: Andersen
- first_name: Svetlana
  full_name: Ulyanchenko, Svetlana
  last_name: Ulyanchenko
- first_name: Pawel J.
  full_name: Schweiger, Pawel J.
  last_name: Schweiger
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Benjamin D.
  full_name: Simons, Benjamin D.
  last_name: Simons
- first_name: Kim B.
  full_name: Jensen, Kim B.
  last_name: Jensen
citation:
  ama: Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB.
    Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth
    and maintenance. <i>Journal of Investigative Dermatology</i>. 2025;145(9):2191-2202.e5.
    doi:<a href="https://doi.org/10.1016/j.jid.2025.01.034">10.1016/j.jid.2025.01.034</a>
  apa: Andersen, M. S., Ulyanchenko, S., Schweiger, P. J., Hannezo, E. B., Simons,
    B. D., &#38; Jensen, K. B. (2025). Spatiotemporal switches in progenitor cell
    fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative
    Dermatology</i>. Elsevier. <a href="https://doi.org/10.1016/j.jid.2025.01.034">https://doi.org/10.1016/j.jid.2025.01.034</a>
  chicago: Andersen, Marianne S., Svetlana Ulyanchenko, Pawel J. Schweiger, Edouard
    B Hannezo, Benjamin D. Simons, and Kim B. Jensen. “Spatiotemporal Switches in
    Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal
    of Investigative Dermatology</i>. Elsevier, 2025. <a href="https://doi.org/10.1016/j.jid.2025.01.034">https://doi.org/10.1016/j.jid.2025.01.034</a>.
  ieee: M. S. Andersen, S. Ulyanchenko, P. J. Schweiger, E. B. Hannezo, B. D. Simons,
    and K. B. Jensen, “Spatiotemporal switches in progenitor cell fate govern upper
    hair follicle growth and maintenance,” <i>Journal of Investigative Dermatology</i>,
    vol. 145, no. 9. Elsevier, p. 2191–2202.e5, 2025.
  ista: Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB.
    2025. Spatiotemporal switches in progenitor cell fate govern upper hair follicle
    growth and maintenance. Journal of Investigative Dermatology. 145(9), 2191–2202.e5.
  mla: Andersen, Marianne S., et al. “Spatiotemporal Switches in Progenitor Cell Fate
    Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative
    Dermatology</i>, vol. 145, no. 9, Elsevier, 2025, p. 2191–2202.e5, doi:<a href="https://doi.org/10.1016/j.jid.2025.01.034">10.1016/j.jid.2025.01.034</a>.
  short: M.S. Andersen, S. Ulyanchenko, P.J. Schweiger, E.B. Hannezo, B.D. Simons,
    K.B. Jensen, Journal of Investigative Dermatology 145 (2025) 2191–2202.e5.
corr_author: '1'
date_created: 2025-04-06T22:01:32Z
date_published: 2025-09-01T00:00:00Z
date_updated: 2025-12-29T14:13:43Z
day: '01'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.jid.2025.01.034
external_id:
  isi:
  - '001604396400001'
  pmid:
  - '40010488'
file:
- access_level: open_access
  checksum: a2b313de3cacb53f20f2b91c42612ad9
  content_type: application/pdf
  creator: dernst
  date_created: 2025-12-29T14:13:01Z
  date_updated: 2025-12-29T14:13:01Z
  file_id: '20874'
  file_name: 2025_JourInvestigativeDerma_Andersen.pdf
  file_size: 7301679
  relation: main_file
  success: 1
file_date_updated: 2025-12-29T14:13:01Z
has_accepted_license: '1'
intvolume: '       145'
isi: 1
issue: '9'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: 2191-2202.e5
pmid: 1
publication: Journal of Investigative Dermatology
publication_identifier:
  eissn:
  - 1523-1747
  issn:
  - 0022-202X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Spatiotemporal switches in progenitor cell fate govern upper hair follicle
  growth and maintenance
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 145
year: '2025'
...