---
_id: '7179'
abstract:
- lang: eng
  text: Glutamate is the major excitatory neurotransmitter in the CNS binding to a
    variety of glutamate receptors. Metabotropic glutamate receptors (mGluR1 to mGluR8)
    can act excitatory or inhibitory, depending on associated signal cascades. Expression
    and localization of inhibitory acting mGluRs at inner hair cells (IHCs) in the
    cochlea are largely unknown. Here, we analyzed expression of mGluR2, mGluR3, mGluR4,
    mGluR6, mGluR7, and mGluR8 and investigated their localization with respect to
    the presynaptic ribbon of IHC synapses. We detected transcripts for mGluR2, mGluR3,
    and mGluR4 as well as for mGluR7a, mGluR7b, mGluR8a, and mGluR8b splice variants.
    Using receptor-specific antibodies in cochlear wholemounts, we found expression
    of mGluR2, mGluR4, and mGluR8b close to presynaptic ribbons. Super resolution
    and confocal microscopy in combination with 3-dimensional reconstructions indicated
    a postsynaptic localization of mGluR2 that overlaps with postsynaptic density
    protein 95 on dendrites of afferent type I spiral ganglion neurons. In contrast,
    mGluR4 and mGluR8b were expressed at the presynapse close to IHC ribbons. In summary,
    we localized in detail 3 mGluR types at IHC ribbon synapses, providing a fundament
    for new therapeutical strategies that could protect the cochlea against noxious
    stimuli and excitotoxicity.
article_processing_charge: No
article_type: original
author:
- first_name: Lisa
  full_name: Klotz, Lisa
  last_name: Klotz
- first_name: Olaf
  full_name: Wendler, Olaf
  last_name: Wendler
- first_name: Renato
  full_name: Frischknecht, Renato
  last_name: Frischknecht
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Holger
  full_name: Schulze, Holger
  last_name: Schulze
- first_name: Ralf
  full_name: Enz, Ralf
  last_name: Enz
citation:
  ama: Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R. Localization
    of group II and III metabotropic glutamate receptors at pre- and postsynaptic
    sites of inner hair cell ribbon synapses. <i>FASEB Journal</i>. 2019;33(12):13734-13746.
    doi:<a href="https://doi.org/10.1096/fj.201901543R">10.1096/fj.201901543R</a>
  apa: Klotz, L., Wendler, O., Frischknecht, R., Shigemoto, R., Schulze, H., &#38;
    Enz, R. (2019). Localization of group II and III metabotropic glutamate receptors
    at pre- and postsynaptic sites of inner hair cell ribbon synapses. <i>FASEB Journal</i>.
    FASEB. <a href="https://doi.org/10.1096/fj.201901543R">https://doi.org/10.1096/fj.201901543R</a>
  chicago: Klotz, Lisa, Olaf Wendler, Renato Frischknecht, Ryuichi Shigemoto, Holger
    Schulze, and Ralf Enz. “Localization of Group II and III Metabotropic Glutamate
    Receptors at Pre- and Postsynaptic Sites of Inner Hair Cell Ribbon Synapses.”
    <i>FASEB Journal</i>. FASEB, 2019. <a href="https://doi.org/10.1096/fj.201901543R">https://doi.org/10.1096/fj.201901543R</a>.
  ieee: L. Klotz, O. Wendler, R. Frischknecht, R. Shigemoto, H. Schulze, and R. Enz,
    “Localization of group II and III metabotropic glutamate receptors at pre- and
    postsynaptic sites of inner hair cell ribbon synapses,” <i>FASEB Journal</i>,
    vol. 33, no. 12. FASEB, pp. 13734–13746, 2019.
  ista: Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R. 2019. Localization
    of group II and III metabotropic glutamate receptors at pre- and postsynaptic
    sites of inner hair cell ribbon synapses. FASEB Journal. 33(12), 13734–13746.
  mla: Klotz, Lisa, et al. “Localization of Group II and III Metabotropic Glutamate
    Receptors at Pre- and Postsynaptic Sites of Inner Hair Cell Ribbon Synapses.”
    <i>FASEB Journal</i>, vol. 33, no. 12, FASEB, 2019, pp. 13734–46, doi:<a href="https://doi.org/10.1096/fj.201901543R">10.1096/fj.201901543R</a>.
  short: L. Klotz, O. Wendler, R. Frischknecht, R. Shigemoto, H. Schulze, R. Enz,
    FASEB Journal 33 (2019) 13734–13746.
date_created: 2019-12-15T23:00:42Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2026-04-03T09:44:03Z
day: '01'
ddc:
- '571'
- '599'
department:
- _id: RySh
doi: 10.1096/fj.201901543R
external_id:
  isi:
  - '000507466100054'
  pmid:
  - '31585509'
file:
- access_level: open_access
  checksum: 79e3b72481dc32489911121cf3b7d8d0
  content_type: application/pdf
  creator: shigemot
  date_created: 2020-12-06T17:30:09Z
  date_updated: 2020-12-06T17:30:09Z
  file_id: '8922'
  file_name: Klotz et al 2019 EMBO Reports.pdf
  file_size: 4766789
  relation: main_file
  success: 1
file_date_updated: 2020-12-06T17:30:09Z
has_accepted_license: '1'
intvolume: '        33'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
page: 13734-13746
pmid: 1
publication: FASEB Journal
publication_identifier:
  eissn:
  - 1530-6860
publication_status: published
publisher: FASEB
quality_controlled: '1'
scopus_import: '1'
status: public
title: Localization of group II and III metabotropic glutamate receptors at pre- and
  postsynaptic sites of inner hair cell ribbon synapses
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 33
year: '2019'
...