[{"file_date_updated":"2025-07-31T07:03:43Z","ec_funded":1,"title":"Super-resolution expansion microscopy in plant roots","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"18689"},{"id":"18837","relation":"research_data","status":"public"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"citation":{"ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>The Plant Cell</i>, vol. 37, no. 4. Oxford University Press, 2025.","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>."},"date_created":"2025-02-05T06:52:06Z","year":"2025","quality_controlled":"1","corr_author":"1","article_type":"original","type":"journal_article","volume":37,"day":"01","publication":"The Plant Cell","issue":"4","publisher":"Oxford University Press","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","_id":"19003","intvolume":"        37","abstract":[{"text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ","lang":"eng"}],"pmid":1,"status":"public","publication_status":"published","file":[{"file_name":"2025_PlantCell_Gallei.pdf","date_created":"2025-07-31T07:03:43Z","content_type":"application/pdf","relation":"main_file","date_updated":"2025-07-31T07:03:43Z","success":1,"creator":"dernst","file_size":53904111,"checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","access_level":"open_access","file_id":"20092"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"article_number":"koaf006","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","doi":"10.1093/plcell/koaf006","month":"04","oa_version":"Published Version","scopus_import":"1","has_accepted_license":"1","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"PlanS_conform":"1","external_id":{"isi":["001462763100001"],"pmid":["39792900"]},"OA_place":"publisher","date_updated":"2025-10-08T08:43:56Z","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"grant_number":"665385","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program"},{"grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships"},{"_id":"269B5B22-B435-11E9-9278-68D0E5697425","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","grant_number":"ALTF 679-2018"},{"_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","grant_number":"26137"},{"name":"Molecular Drug Targets","call_identifier":"FWF","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24"}],"date_published":"2025-04-01T00:00:00Z","ddc":["580"],"author":[{"first_name":"Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sven M","last_name":"Truckenbrodt","full_name":"Truckenbrodt, Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0009-0002-5890-120X","first_name":"Syamala","last_name":"Inumella","id":"F8660870-D756-11E9-98C5-34DFE5697425","full_name":"Inumella, Syamala"},{"full_name":"Vistunou, Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali"},{"first_name":"Christoph M","orcid":"0000-0003-1216-9105","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"last_name":"Tavakoli","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba","orcid":"0000-0002-7667-6854"},{"full_name":"Agudelo Duenas, Nathalie","last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","first_name":"Nathalie"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","full_name":"Vorlaufer, Jakob","last_name":"Vorlaufer","orcid":"0009-0000-7590-3501","first_name":"Jakob"},{"last_name":"Jahr","full_name":"Jahr, Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","first_name":"Wiebke"},{"first_name":"Marek","last_name":"Randuch","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","full_name":"Johnson, Alexander J"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Johann G","orcid":"0000-0001-8559-3973","last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G"}]},{"citation":{"short":"L.-Z. Zhou, L. Wang, X. Chen, Z. Ge, J. Mergner, X. Li, B. Küster, G. Längst, L.-J. Qu, T. Dresselhaus, The Plant Cell 36 (2024).","ieee":"L.-Z. Zhou <i>et al.</i>, “The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize,” <i>The Plant Cell</i>, vol. 36, no. 5. Oxford University Press, 2024.","mla":"Zhou, Liang-Zi, et al. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>, vol. 36, no. 5, koad324, Oxford University Press, 2024, doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>.","apa":"Zhou, L.-Z., Wang, L., Chen, X., Ge, Z., Mergner, J., Li, X., … Dresselhaus, T. (2024). The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>","chicago":"Zhou, Liang-Zi, Lele Wang, Xia Chen, Zengxiang Ge, Julia Mergner, Xingli Li, Bernhard Küster, Gernot Längst, Li-Jia Qu, and Thomas Dresselhaus. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>.","ama":"Zhou L-Z, Wang L, Chen X, et al. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. 2024;36(5). doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>","ista":"Zhou L-Z, Wang L, Chen X, Ge Z, Mergner J, Li X, Küster B, Längst G, Qu L-J, Dresselhaus T. 2024. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. The Plant Cell. 36(5), koad324."},"oa":1,"article_type":"original","quality_controlled":"1","year":"2024","date_created":"2024-01-02T11:19:37Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize","main_file_link":[{"url":"https://doi.org/10.1093/plcell/koad324","open_access":"1"}],"status":"public","_id":"14726","pmid":1,"abstract":[{"text":"Autocrine signaling pathways regulated by RAPID ALKALINIZATION FACTORs (RALFs) control cell wall integrity during pollen tube germination and growth in Arabidopsis (Arabidopsis thaliana). To investigate the role of pollen-specific RALFs in another plant species, we combined gene expression data with phylogenetic and biochemical studies to identify candidate orthologs in maize (Zea mays). We show that Clade IB ZmRALF2/3 mutations, but not Clade III ZmRALF1/5 mutations, cause cell wall instability in the sub-apical region of the growing pollen tube. ZmRALF2/3 are mainly located in the cell wall and are partially able to complement the pollen germination defect of their Arabidopsis orthologs AtRALF4/19. Mutations in ZmRALF2/3 compromise pectin distribution patterns leading to altered cell wall organization and thickness culminating in pollen tube burst. Clade IB, but not Clade III ZmRALFs, strongly interact as ligands with the pollen-specific Catharanthus roseus RLK1-like (CrRLK1L) receptor kinases Zea mays FERONIA-like (ZmFERL) 4/7/9, LORELEI-like glycosylphosphatidylinositol-anchor (LLG) proteins Zea mays LLG 1 and 2 (ZmLLG1/2) and Zea mays pollen extension-like (PEX) cell wall proteins ZmPEX2/4. Notably, ZmFERL4 outcompetes ZmLLG2 and ZmPEX2 outcompetes ZmFERL4 for ZmRALF2 binding. Based on these data, we suggest that Clade IB RALFs act in a dual role as cell wall components and extracellular sensors to regulate cell wall integrity and thickness during pollen tube growth in maize and probably other plants.","lang":"eng"}],"intvolume":"        36","publication_status":"published","day":"01","keyword":["Cell Biology","Plant Science"],"volume":36,"type":"journal_article","article_processing_charge":"No","publisher":"Oxford University Press","publication":"The Plant Cell","issue":"5","extern":"1","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"doi":"10.1093/plcell/koad324","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"article_number":"koad324","date_updated":"2024-07-16T11:18:46Z","author":[{"first_name":"Liang-Zi","last_name":"Zhou","full_name":"Zhou, Liang-Zi"},{"last_name":"Wang","full_name":"Wang, Lele","first_name":"Lele"},{"full_name":"Chen, Xia","last_name":"Chen","first_name":"Xia"},{"orcid":"0000-0001-9381-3577","first_name":"Zengxiang","full_name":"Ge, Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge"},{"first_name":"Julia","last_name":"Mergner","full_name":"Mergner, Julia"},{"first_name":"Xingli","last_name":"Li","full_name":"Li, Xingli"},{"first_name":"Bernhard","last_name":"Küster","full_name":"Küster, Bernhard"},{"full_name":"Längst, Gernot","last_name":"Längst","first_name":"Gernot"},{"last_name":"Qu","full_name":"Qu, Li-Jia","first_name":"Li-Jia"},{"first_name":"Thomas","full_name":"Dresselhaus, Thomas","last_name":"Dresselhaus"}],"ddc":["580"],"date_published":"2024-05-01T00:00:00Z","has_accepted_license":"1","scopus_import":"1","oa_version":"Published Version","month":"05","external_id":{"pmid":["38142229"]},"language":[{"iso":"eng"}]},{"doi":"10.1093/plcell/koad319","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"department":[{"_id":"JiFr"}],"ddc":["580"],"date_published":"2024-04-01T00:00:00Z","author":[{"last_name":"Huebbers","full_name":"Huebbers, Jan W.","first_name":"Jan W."},{"last_name":"Caldarescu","full_name":"Caldarescu, George A.","first_name":"George A."},{"full_name":"Kubátová, Zdeňka","last_name":"Kubátová","first_name":"Zdeňka"},{"last_name":"Sabol","full_name":"Sabol, Peter","first_name":"Peter"},{"last_name":"Levecque","full_name":"Levecque, Sophie C.J.","first_name":"Sophie C.J."},{"full_name":"Kuhn, Hannah","last_name":"Kuhn","first_name":"Hannah"},{"full_name":"Kulich, Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","first_name":"Ivan"},{"full_name":"Reinstädler, Anja","last_name":"Reinstädler","first_name":"Anja"},{"full_name":"Büttgen, Kim","last_name":"Büttgen","first_name":"Kim"},{"last_name":"Manga-Robles","full_name":"Manga-Robles, Alba","first_name":"Alba"},{"first_name":"Hugo","full_name":"Mélida, Hugo","last_name":"Mélida"},{"full_name":"Pauly, Markus","last_name":"Pauly","first_name":"Markus"},{"first_name":"Ralph","full_name":"Panstruga, Ralph","last_name":"Panstruga"},{"full_name":"Žárský, Viktor","last_name":"Žárský","first_name":"Viktor"}],"date_updated":"2024-04-17T07:01:21Z","language":[{"iso":"eng"}],"external_id":{"pmid":["38124479"]},"month":"04","has_accepted_license":"1","oa_version":"Published Version","scopus_import":"1","year":"2024","date_created":"2024-04-14T22:01:02Z","article_type":"original","quality_controlled":"1","oa":1,"citation":{"ieee":"J. W. Huebbers <i>et al.</i>, “Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew,” <i>Plant Cell</i>, vol. 36, no. 4. Oxford University Press, pp. 1007–1035, 2024.","mla":"Huebbers, Jan W., et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>, vol. 36, no. 4, Oxford University Press, 2024, pp. 1007–35, doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>.","short":"J.W. Huebbers, G.A. Caldarescu, Z. Kubátová, P. Sabol, S.C.J. Levecque, H. Kuhn, I. Kulich, A. Reinstädler, K. Büttgen, A. Manga-Robles, H. Mélida, M. Pauly, R. Panstruga, V. Žárský, Plant Cell 36 (2024) 1007–1035.","apa":"Huebbers, J. W., Caldarescu, G. A., Kubátová, Z., Sabol, P., Levecque, S. C. J., Kuhn, H., … Žárský, V. (2024). Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>","chicago":"Huebbers, Jan W., George A. Caldarescu, Zdeňka Kubátová, Peter Sabol, Sophie C.J. Levecque, Hannah Kuhn, Ivan Kulich, et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>.","ama":"Huebbers JW, Caldarescu GA, Kubátová Z, et al. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. 2024;36(4):1007-1035. doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>","ista":"Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský V. 2024. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. 36(4), 1007–1035."},"title":"Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew","file_date_updated":"2024-04-17T06:57:54Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"content_type":"application/pdf","relation":"main_file","date_created":"2024-04-17T06:57:54Z","file_name":"2024_PlantCell_Huebbers.pdf","creator":"dernst","success":1,"date_updated":"2024-04-17T06:57:54Z","checksum":"f9994d2e4748feec24c992b39871aba1","file_size":2866098,"file_id":"15326","access_level":"open_access"}],"publication_status":"published","status":"public","abstract":[{"lang":"eng","text":"Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and Fourier transform infrared spectroscopic analyses corroborated deficiencies in the composition of trichome cell walls in these mutants. Transgenic lines expressing fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant. Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo triple mutant plants. In vivo protein–protein interaction assays in plant and yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed synergistically enhanced resistance to powdery mildew attack. Taken together, our data point to an isoform-specific interplay of EXO70 and MLO proteins in the modulation of trichome cell wall biogenesis and powdery mildew susceptibility."}],"_id":"15319","pmid":1,"intvolume":"        36","publisher":"Oxford University Press","publication":"Plant Cell","issue":"4","article_processing_charge":"Yes (in subscription journal)","type":"journal_article","page":"1007-1035","day":"01","volume":36},{"date_updated":"2025-09-08T07:21:17Z","OA_place":"publisher","ddc":["580"],"date_published":"2024-05-01T00:00:00Z","author":[{"last_name":"He","full_name":"He, Shengbo","first_name":"Shengbo"},{"first_name":"Yiming","id":"318e643b-8b61-11ed-b69e-aafa103ec8dd","full_name":"Yu, Yiming","last_name":"Yu"},{"first_name":"Liang","last_name":"Wang","full_name":"Wang, Liang"},{"first_name":"Jingyi","full_name":"Zhang, Jingyi","last_name":"Zhang"},{"last_name":"Bai","full_name":"Bai, Zhengyong","first_name":"Zhengyong"},{"first_name":"Guohong","last_name":"Li","full_name":"Li, Guohong"},{"full_name":"Li, Pilong","last_name":"Li","first_name":"Pilong"},{"full_name":"Feng, Xiaoqi","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","last_name":"Feng","first_name":"Xiaoqi","orcid":"0000-0002-4008-1234"}],"month":"05","has_accepted_license":"1","oa_version":"Published Version","scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"}],"language":[{"iso":"eng"}],"external_id":{"pmid":["38309957"],"isi":["001180817000001"]},"publication_identifier":{"eissn":["1532-298X"]},"acknowledgement":"This work was funded by ISTA core support (Y.Y. and X.F.) and grants from the National Natural Science Foundation of China (31871443 to L.W. and P.L.; 32100417 to L.W.).\r\nWe thank the ISTA Imaging and Optics Facility for assistance with microscopy and the ISTA Scientific Computing Facility for high-performance computing resources.","doi":"10.1093/plcell/koae034","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"department":[{"_id":"XiFe"}],"isi":1,"status":"public","pmid":1,"_id":"15375","abstract":[{"text":"In the eukaryotic nucleus, heterochromatin forms highly condensed, visible foci known as heterochromatin foci (HF). These HF are enriched with linker histone H1, a key player in heterochromatin condensation and silencing. However, it is unknown how H1 aggregates HF and condenses heterochromatin. In this study, we established that H1 facilitates heterochromatin condensation by enhancing inter- and intrachromosomal interactions between and within heterochromatic regions of the Arabidopsis (Arabidopsis thaliana) genome. We demonstrated that H1 drives HF formation via phase separation, which requires its C-terminal intrinsically disordered region (C-IDR). A truncated H1 lacking the C-IDR fails to form foci or recover HF in the h1 mutant background, whereas C-IDR with a short stretch of the globular domain (18 out of 71 amino acids) is sufficient to rescue both defects. In addition, C-IDR is essential for H1's roles in regulating nucleosome repeat length and DNA methylation in Arabidopsis, indicating that phase separation capability is required for chromatin functions of H1. Our data suggest that bacterial H1-like proteins, which have been shown to condense DNA, are intrinsically disordered and capable of mediating phase separation. Therefore, we propose that phase separation mediated by H1 or H1-like proteins may represent an ancient mechanism for condensing chromatin and DNA.","lang":"eng"}],"intvolume":"        36","file":[{"checksum":"eed76c848fe3d8fe9a53943181aaa53c","file_size":50791962,"access_level":"open_access","file_id":"19611","date_created":"2025-04-23T07:43:12Z","file_name":"2024_PlantCell_He.pdf","content_type":"application/pdf","relation":"main_file","date_updated":"2025-04-23T07:43:12Z","success":1,"creator":"dernst"}],"publication_status":"published","type":"journal_article","day":"01","page":"1829-1843","volume":36,"publisher":"Oxford University Press","issue":"5","publication":"The Plant Cell","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","oa":1,"citation":{"ista":"He S, Yu Y, Wang L, Zhang J, Bai Z, Li G, Li P, Feng X. 2024. Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis. The Plant Cell. 36(5), 1829–1843.","ama":"He S, Yu Y, Wang L, et al. Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis. <i>The Plant Cell</i>. 2024;36(5):1829-1843. doi:<a href=\"https://doi.org/10.1093/plcell/koae034\">10.1093/plcell/koae034</a>","chicago":"He, Shengbo, Yiming Yu, Liang Wang, Jingyi Zhang, Zhengyong Bai, Guohong Li, Pilong Li, and Xiaoqi Feng. “Linker Histone H1 Drives Heterochromatin Condensation via Phase Separation in Arabidopsis.” <i>The Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koae034\">https://doi.org/10.1093/plcell/koae034</a>.","apa":"He, S., Yu, Y., Wang, L., Zhang, J., Bai, Z., Li, G., … Feng, X. (2024). Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koae034\">https://doi.org/10.1093/plcell/koae034</a>","ieee":"S. He <i>et al.</i>, “Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis,” <i>The Plant Cell</i>, vol. 36, no. 5. Oxford University Press, pp. 1829–1843, 2024.","mla":"He, Shengbo, et al. “Linker Histone H1 Drives Heterochromatin Condensation via Phase Separation in Arabidopsis.” <i>The Plant Cell</i>, vol. 36, no. 5, Oxford University Press, 2024, pp. 1829–43, doi:<a href=\"https://doi.org/10.1093/plcell/koae034\">10.1093/plcell/koae034</a>.","short":"S. He, Y. Yu, L. Wang, J. Zhang, Z. Bai, G. Li, P. Li, X. Feng, The Plant Cell 36 (2024) 1829–1843."},"year":"2024","date_created":"2024-05-12T22:01:01Z","article_type":"original","corr_author":"1","quality_controlled":"1","title":"Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis","file_date_updated":"2025-04-23T07:43:12Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"issue":"6","extern":"1","publication":"The Plant Cell","publisher":"Oxford University Press","article_processing_charge":"No","type":"journal_article","volume":35,"day":"01","keyword":["Cell Biology","Plant Science"],"publication_status":"published","abstract":[{"text":"The study of RNAs has become one of the most influential research fields in contemporary biology and biomedicine. In the last few years, new sequencing technologies have produced an explosion of new and exciting discoveries in the field but have also given rise to many open questions. Defining these questions, together with old, long-standing gaps in our knowledge, is the spirit of this article. The breadth of topics within RNA biology research is vast, and every aspect of the biology of these molecules contains countless exciting open questions. Here, we asked 12 groups to discuss their most compelling question among some plant RNA biology topics. The following vignettes cover RNA alternative splicing; RNA dynamics; RNA translation; RNA structures; R-loops; epitranscriptomics; long non-coding RNAs; small RNA production and their functions in crops; small RNAs during gametogenesis and in cross-kingdom RNA interference; and RNA-directed DNA methylation. In each section, we will present the current state-of-the-art in plant RNA biology research before asking the questions that will surely motivate future discoveries in the field. We hope this article will spark a debate about the future perspective on RNA biology and provoke novel reflections in the reader.","lang":"eng"}],"_id":"12669","pmid":1,"intvolume":"        35","status":"public","main_file_link":[{"url":"https://doi.org/10.1093/plcell/koac346","open_access":"1"}],"title":"Beyond transcription: compelling open questions in plant RNA biology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2023-02-23T09:14:59Z","year":"2023","quality_controlled":"1","article_type":"original","oa":1,"citation":{"short":"P.A. Manavella, M.A. Godoy Herz, A.R. Kornblihtt, R. Sorenson, L.E. Sieburth, K. Nakaminami, M. Seki, Y. Ding, Q. Sun, H. Kang, F.D. Ariel, M. Crespi, A.J. Giudicatti, Q. Cai, H. Jin, X. Feng, Y. Qi, C.S. Pikaard, The Plant Cell 35 (2023).","ieee":"P. A. Manavella <i>et al.</i>, “Beyond transcription: compelling open questions in plant RNA biology,” <i>The Plant Cell</i>, vol. 35, no. 6. Oxford University Press, 2023.","mla":"Manavella, Pablo A., et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>, vol. 35, no. 6, koac346, Oxford University Press, 2023, doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>.","ama":"Manavella PA, Godoy Herz MA, Kornblihtt AR, et al. Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. 2023;35(6). doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>","ista":"Manavella PA, Godoy Herz MA, Kornblihtt AR, Sorenson R, Sieburth LE, Nakaminami K, Seki M, Ding Y, Sun Q, Kang H, Ariel FD, Crespi M, Giudicatti AJ, Cai Q, Jin H, Feng X, Qi Y, Pikaard CS. 2023. Beyond transcription: compelling open questions in plant RNA biology. The Plant Cell. 35(6), koac346.","apa":"Manavella, P. A., Godoy Herz, M. A., Kornblihtt, A. R., Sorenson, R., Sieburth, L. E., Nakaminami, K., … Pikaard, C. S. (2023). Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>","chicago":"Manavella, Pablo A, Micaela A Godoy Herz, Alberto R Kornblihtt, Reed Sorenson, Leslie E Sieburth, Kentaro Nakaminami, Motoaki Seki, et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>."},"language":[{"iso":"eng"}],"external_id":{"pmid":["36477566"]},"month":"06","oa_version":"Published Version","scopus_import":"1","date_published":"2023-06-01T00:00:00Z","author":[{"first_name":"Pablo A","full_name":"Manavella, Pablo A","last_name":"Manavella"},{"last_name":"Godoy Herz","full_name":"Godoy Herz, Micaela A","first_name":"Micaela A"},{"full_name":"Kornblihtt, Alberto R","last_name":"Kornblihtt","first_name":"Alberto R"},{"full_name":"Sorenson, Reed","last_name":"Sorenson","first_name":"Reed"},{"full_name":"Sieburth, Leslie E","last_name":"Sieburth","first_name":"Leslie E"},{"full_name":"Nakaminami, Kentaro","last_name":"Nakaminami","first_name":"Kentaro"},{"first_name":"Motoaki","last_name":"Seki","full_name":"Seki, Motoaki"},{"first_name":"Yiliang","last_name":"Ding","full_name":"Ding, Yiliang"},{"last_name":"Sun","full_name":"Sun, Qianwen","first_name":"Qianwen"},{"first_name":"Hunseung","full_name":"Kang, Hunseung","last_name":"Kang"},{"full_name":"Ariel, Federico D","last_name":"Ariel","first_name":"Federico D"},{"last_name":"Crespi","full_name":"Crespi, Martin","first_name":"Martin"},{"first_name":"Axel J","last_name":"Giudicatti","full_name":"Giudicatti, Axel J"},{"first_name":"Qiang","last_name":"Cai","full_name":"Cai, Qiang"},{"full_name":"Jin, Hailing","last_name":"Jin","first_name":"Hailing"},{"full_name":"Feng, Xiaoqi","last_name":"Feng","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","first_name":"Xiaoqi","orcid":"0000-0002-4008-1234"},{"full_name":"Qi, Yijun","last_name":"Qi","first_name":"Yijun"},{"last_name":"Pikaard","full_name":"Pikaard, Craig S","first_name":"Craig S"}],"date_updated":"2023-10-04T09:48:43Z","article_number":"koac346","department":[{"_id":"XiFe"}],"doi":"10.1093/plcell/koac346","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]}},{"oa":1,"citation":{"short":"Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao, The Plant Cell 34 (2022) 4816–4839.","mla":"Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” <i>The Plant Cell</i>, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39, doi:<a href=\"https://doi.org/10.1093/plcell/koac270\">10.1093/plcell/koac270</a>.","ieee":"Z. Tian <i>et al.</i>, “Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),” <i>The Plant Cell</i>, vol. 34, no. 12. Oxford University Press, pp. 4816–4839, 2022.","ama":"Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). <i>The Plant Cell</i>. 2022;34(12):4816-4839. doi:<a href=\"https://doi.org/10.1093/plcell/koac270\">10.1093/plcell/koac270</a>","ista":"Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.","apa":"Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022). Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac270\">https://doi.org/10.1093/plcell/koac270</a>","chicago":"Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” <i>The Plant Cell</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/plcell/koac270\">https://doi.org/10.1093/plcell/koac270</a>."},"year":"2022","date_created":"2022-09-07T14:19:39Z","article_type":"original","quality_controlled":"1","title":"Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum)","file_date_updated":"2023-01-20T08:29:12Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","related_material":{"link":[{"url":"https://doi.org/10.1093/plcell/koac342","relation":"erratum"}]},"status":"public","_id":"12053","abstract":[{"text":"Strigolactones (SLs) are a class of phytohormones that regulate plant shoot branching and adventitious root development. However, little is known regarding the role of SLs in controlling the behavior of the smallest unit of the organism, the single cell. Here, taking advantage of a classic single-cell model offered by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs) and cellulose, respectively. Furthermore, we identified two layers of transcription factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk. The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase (CesA) genes by directly activating their transcription. Finally, the KCS and CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose, respectively, to regulate development of high-grade cotton fibers. In addition to providing a theoretical basis for cotton fiber improvement, our results shed light on SL signaling in plant development at the single-cell level.","lang":"eng"}],"intvolume":"        34","pmid":1,"file":[{"content_type":"application/pdf","relation":"main_file","date_created":"2023-01-20T08:29:12Z","file_name":"2022_PlantCell_Tian.pdf","success":1,"creator":"dernst","date_updated":"2023-01-20T08:29:12Z","checksum":"1c606d9545f29dfca15235f69ad27b58","file_size":3282540,"file_id":"12318","access_level":"open_access"}],"publication_status":"published","type":"journal_article","page":"4816-4839","day":"01","volume":34,"publisher":"Oxford University Press","issue":"12","publication":"The Plant Cell","article_processing_charge":"No","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"acknowledgement":"This work was supported by the National Natural Science Foundation of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental Research Funds for the Central Universities (GK202002005 and GK202201017), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12 and CB2021A21) and FWF Stand-alone Project (P29988).","doi":"10.1093/plcell/koac270","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"department":[{"_id":"JiFr"}],"isi":1,"date_updated":"2025-04-15T08:12:07Z","project":[{"name":"RNA-directed DNA methylation in plant development","call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988"}],"ddc":["580"],"date_published":"2022-12-01T00:00:00Z","author":[{"first_name":"Z","full_name":"Tian, Z","last_name":"Tian"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou","orcid":"0000-0003-2627-6956"},{"last_name":"Zhu","full_name":"Zhu, L","first_name":"L"},{"last_name":"Jiang","full_name":"Jiang, B","first_name":"B"},{"last_name":"Wang","full_name":"Wang, H","first_name":"H"},{"full_name":"Gao, R","last_name":"Gao","first_name":"R"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"first_name":"G","full_name":"Xiao, G","last_name":"Xiao"}],"month":"12","has_accepted_license":"1","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"external_id":{"isi":["000852753000001"],"pmid":["36040191"]}},{"scopus_import":"1","oa_version":"Published Version","month":"05","external_id":{"pmid":["32193204"],"isi":["000545741500030"]},"acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"date_updated":"2025-04-14T07:45:03Z","project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"author":[{"id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","last_name":"Zhang","full_name":"Zhang, Xixi","first_name":"Xixi","orcid":"0000-0001-7048-4627"},{"first_name":"Maciek","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski"},{"last_name":"Marhavá","full_name":"Marhavá, Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan"},{"full_name":"Zhang, Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey","first_name":"Lesia","orcid":"0000-0002-7244-7237"},{"first_name":"Marta","last_name":"Zwiewka","full_name":"Zwiewka, Marta"},{"full_name":"Pukyšová, Vendula","last_name":"Pukyšová","first_name":"Vendula"},{"first_name":"Adrià Sans","full_name":"Sánchez, Adrià Sans","last_name":"Sánchez"},{"first_name":"Vivek Kumar","full_name":"Raxwal, Vivek Kumar","last_name":"Raxwal"},{"full_name":"Hardtke, Christian S.","last_name":"Hardtke","first_name":"Christian S."},{"first_name":"Tomasz","full_name":"Nodzynski, Tomasz","last_name":"Nodzynski"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"date_published":"2020-05-01T00:00:00Z","isi":1,"department":[{"_id":"JiFr"}],"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"doi":"10.1105/tpc.19.00869","day":"01","page":"1644-1664","volume":32,"type":"journal_article","article_processing_charge":"No","publisher":"American Society of Plant Biologists","publication":"The Plant Cell","issue":"5","main_file_link":[{"url":"https://doi.org/10.1105/tpc.19.00869","open_access":"1"}],"status":"public","_id":"7619","intvolume":"        32","pmid":1,"abstract":[{"lang":"eng","text":"Cell polarity is a fundamental feature of all multicellular organisms. In plants, prominent cell polarity markers are PIN auxin transporters crucial for plant development. To identify novel components involved in cell polarity establishment and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2 Arabidopsis plants, which ectopically express predominantly basally localized PIN1 in the root epidermal cells leading to agravitropic root growth. From the screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused PIN1-HA polarity switch from basal to apical side of root epidermal cells. Complementation experiments established the repp12 causative mutation as an amino acid substitution in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation. ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple mutants confirmed a crucial role of ALA proteins in regulating plant development and in PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together, our results identified ALA3 flippase as an important interactor and regulator of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development."}],"publication_status":"published","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ec_funded":1,"title":"Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters","citation":{"short":"X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey, M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski, J. Friml, The Plant Cell 32 (2020) 1644–1664.","ieee":"X. Zhang <i>et al.</i>, “Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters,” <i>The Plant Cell</i>, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664, 2020.","mla":"Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp. 1644–64, doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>.","ama":"Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. 2020;32(5):1644-1664. doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>","ista":"Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.","apa":"Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey, L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>","chicago":"Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang, Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>."},"oa":1,"article_type":"original","quality_controlled":"1","corr_author":"1","year":"2020","date_created":"2020-03-28T07:39:22Z"},{"type":"journal_article","page":"700 - 716","day":"09","volume":30,"publisher":"American Society of Plant Biologists","issue":"3","publication":"The Plant Cell","article_processing_charge":"No","status":"public","pmid":1,"_id":"412","intvolume":"        30","abstract":[{"text":"Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterised compared to that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologues of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2) loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the on-going characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in A. thaliana.","lang":"eng"}],"file":[{"file_id":"11406","access_level":"open_access","file_size":4407538,"checksum":"4e165e653b67d3f0684697f21aace5a1","creator":"dernst","success":1,"date_updated":"2022-05-23T09:12:38Z","content_type":"application/pdf","relation":"main_file","file_name":"2018_PlantCell_Adamowski.pdf","date_created":"2022-05-23T09:12:38Z"}],"publication_status":"published","ec_funded":1,"title":"A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis","file_date_updated":"2022-05-23T09:12:38Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"record":[{"status":"public","id":"6269","relation":"dissertation_contains"}]},"publist_id":"7417","oa":1,"citation":{"ista":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. The Plant Cell. 30(3), 700–716.","ama":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>","chicago":"Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc, Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2018. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>.","apa":"Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml, J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>","short":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml, The Plant Cell 30 (2018) 700–716.","mla":"Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no. 3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>.","ieee":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml, “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant Biologists, pp. 700–716, 2018."},"year":"2018","date_created":"2018-12-11T11:46:20Z","article_type":"original","corr_author":"1","quality_controlled":"1","month":"04","has_accepted_license":"1","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"external_id":{"isi":["000429441400018"],"pmid":["29511054"]},"project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"date_updated":"2026-04-26T22:30:48Z","ddc":["580"],"date_published":"2018-04-09T00:00:00Z","author":[{"full_name":"Adamowski, Maciek","last_name":"Adamowski","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257","first_name":"Maciek"},{"orcid":"0000-0002-8600-0671","first_name":"Madhumitha","full_name":"Narasimhan, Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","last_name":"Narasimhan"},{"first_name":"Urszula","full_name":"Kania, Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","last_name":"Kania"},{"full_name":"Glanc, Matous","last_name":"Glanc","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783","first_name":"Matous"},{"first_name":"Geert","last_name":"De Jaeger","full_name":"De Jaeger, Geert"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"department":[{"_id":"JiFr"}],"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"acknowledgement":"We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9 construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek, Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych, Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for help with correcting the manuscript. This work was supported by the European Research Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project NPUI-LO1417.","doi":"10.1105/tpc.17.00785"}]